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https://www.readbyqxmd.com/read/28905700/roseomonas-aerofrigidensis-sp-nov-isolated-from-an-air-conditioner
#1
Jong Woo Hyeon, Che Ok Jeon
A Gram-stain-negative, strictly aerobic bacterium, designated HC1T, was isolated from an air conditioner in South Korea. Cells were orange, non-motile cocci with oxidase- and catalase-positive activities and did not contain bacteriochlorophyll a. Growth of strain HC1T was observed at 10-45 °C (optimum, 30 °C), pH 4.5-9.5 (optimum, pH 7.0) and 0-3 % (w/v) NaCl (optimum, 0 %). Strain HC1T contained summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), C16 : 0 and cyclo-C19 : 0ω8c as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone...
September 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28853686/serratia-oryzae-sp-nov-isolated-from-rice-stems
#2
Cai-Wen Zhang, Jun Zhang, Juan-Juan Zhao, Xia Zhao, Dong-Fang Zhao, Hua-Qun Yin, Xiao-Xia Zhang
A novel endophytic bacterium, strain J11-6T, was isolated from rice stems. Its taxonomic position was investigated using a polyphasic approach. The novel strain was Gram-staining-negative, facultatively anaerobic, motile and rod-shaped. Although the results of phylogenetic analysis based on 16S rRNA gene sequences indicated that J11-6T represented a member of the genus Rahnella, multilocus sequence analysis (MLSA) on the basis of concatenated partial atpD, gyrB, rpoB and infB gene sequences showed a clear distinction of J11-6T from the type strains of species of the genus Rahnella but indicated that it lay within the clade of the genus Serratia...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820105/roseomonas-hibiscisoli-sp-nov-isolated-from-the-rhizosphere-of-mugunghwa-hibiscus-syriacus
#3
Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Qi-Jun Wang, Tae-Hoo Yi
A Gram-stain-negative, aerobic, non-motile and coccoid to short-rod-shaped bacterial strain (THG-N2.22T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 20-40 °C (optimum 28 °C), at pH 5-9 (optimum 7) and with 0-4 % (w/v) NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-N2.22T were identified as Roseomonas rhizosphaerae YW11T (98.5 % similarity), Roseomonas rubra S5T (98.5 %), Roseomonas cervicalis ATCC 49957T (98...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28818885/draft-genome-sequences-of-two-gammaproteobacterial-methanotrophs-isolated-from-rice-ecosystems
#4
Katharina Frindte, Marina G Kalyuzhnaya, Françoise Bringel, Peter F Dunfield, Mike S M Jetten, Valentina N Khmelenina, Martin G Klotz, J Colin Murrell, Huub J M Op den Camp, Yasuyoshi Sakai, Jeremy D Semrau, Nicole Shapiro, Alan A DiSpirito, Lisa Y Stein, Mette M Svenning, Yuri A Trotsenko, Stéphane Vuilleumier, Tanja Woyke, Claudia Knief
The genomes of the aerobic methanotrophs "Methyloterricola oryzae" strain 73a(T) and Methylomagnum ishizawai strain 175 were sequenced. Both strains were isolated from rice plants. Methyloterricola oryzae strain 73a(T) represents the first isolate of rice paddy cluster I, and strain 175 is the second representative of the recently described genus Methylomagnum.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28755328/alternaria-toxins-in-south-african-sunflower-seeds-cooperative-study
#5
Sebastian Hickert, Lena Hermes, Lucas Maciel Mauriz Marques, Christine Focke, Benedikt Cramer, Norberto Peporine Lopes, Bradley Flett, Hans-Ulrich Humpf
Sunflower seed samples (N = 80) from different sunflower cultivars originating from different localities in South Africa were analyzed for 15 toxins produced by fungi of the genus Alternaria by means of a simple one-step extraction dilute-and-shoot HPLC-MS/MS approach. References for valine-tenuazonic acid (Val-TeA), altenusin (ALTS), and altenuisol (ALTSOH) were isolated from fungal culture extracts and spectroscopically characterized. Additionally, valine-tenuazonic acid was tested regarding its cytotoxicity in comparison with tenuazonic acid (TeA) and showed less activity on HT-29 cells...
July 28, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28736890/insect-frass-in-stored-cereal-products-as-a-potential-source-of-lactobacillus-sanfranciscensis-for-sourdough-ecosystem
#6
Federica Boiocchi, Davide Porcellato, Lidia Limonta, Claudia Picozzi, Ileana Vigentini, Daria Patrizia Locatelli, Roberto Foschino
AIM: Insect frass samples were collected from Drosophila melanogaster, Plodia interpunctella, Rhyzopertha dominica, Sitophilus granarius, S. oryzae, S. zeamais, Tribolium confusum and T. castaneum to elucidate if they can be the origin of Type I sourdough microorganisms (Lactobacillus sanfranciscensis and Candida milleri). METHODS AND RESULTS: Selective enrichments were carried out to isolate Lactic Acid Bacteria and yeast. A metagenetic analysis, targeted on bacterial 16S rRNA gene and fungal ITS region, was performed by using Illumina MiSeq protocol...
July 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28732553/diversity-application-and-synthetic-biology-of-industrially-important-aspergillus-fungi
#7
REVIEW
Hee-Soo Park, Sang-Cheol Jun, Kap-Hoon Han, Seung-Beom Hong, Jae-Hyuk Yu
The filamentous fungal genus Aspergillus consists of over 340 officially recognized species. A handful of these Aspergillus fungi are predominantly used for food fermentation and large-scale production of enzymes, organic acids, and bioactive compounds. These industrially important Aspergilli primarily belong to the two major Aspergillus sections, Nigri and Flavi. Aspergillus oryzae (section Flavi) is the most commonly used mold for the fermentation of soybeans, rice, grains, and potatoes. Aspergillus niger (section Nigri) is used in the industrial production of various enzymes and organic acids, including 99% (1...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28714843/roseomonas-suffusca-sp-nov-isolated-from-lagoon-sediments
#8
Y Subhash, Sang-Seob Lee
Three light-brown-coloured, Gram-stain-negative, small rod- to oval-shaped, motile bacteria were isolated from lagoon sediments collected from North Carolina, USA. The strains (S1T, AS3 and AS6) grew aerobically at 13-42 °C (optimum, 30-35 °C) and pH 6.5-9.1 (optimum, pH 7.0-7.5). All three strains were positive for catalase and oxidase activity, and no added NaCl was required for growth. C18 : 1ω6c/C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c, C18 : 1ω7c 11-methyl and C16 : 0 were the predominant fatty acids (>5 %)...
July 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28643580/cyclophilins-less-studied-proteins-with-critical-roles-in-pathogenesis
#9
Khushwant Singh, Mark Winter, Miloslav Zouhar, Pavel Rysanek
Cyclophilins (EC 5.2.1.8) (CYPs) belong to a subgroup of proteins known as immunophilins, which also include FK-506 binding proteins (FKBPs) and parvulins. Members of the immunophilins have two main characteristic properties: I) peptidyl-prolyl cis-trans isomerase activity (PPIases) and II) the ability to bind immunosuppressant molecules of fungal origin. Cyclophilins are some of the most conserved proteins present in eukaryotes and prokaryotes, and they have been implicated in diverse cellular processes and responses to multiple biotic and abiotic stresses...
June 23, 2017: Phytopathology
https://www.readbyqxmd.com/read/28621110/characterization-of-microbial-communities-in-chinese-rice-wine-collected-at-yichang-city-and-suzhou-city-in-china
#10
Yucai Lü, Yanli Gong, Yajie Li, Zejiang Pan, Yi Yao, Ning Li, Jinling Guo, Dachun Gong, Yihong Tian, Caiyun Peng
Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 days. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and 292.50 and 12.31 mg/g, respectively, for rice wine from Suzhou city. Acetic acid and lactic acid were the most abundant organic acids. Abundant fungi and bacteria were detected in both communities by high-throughput sequencing...
August 28, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28619808/first-insights-into-the-genome-sequence-of-clostridium-oryzae-dsm-28571-isolated-from-the-soil-of-a-japanese-rice-field
#11
Anja Poehlein, Anna Lena Gippert, Maaike J Bierenbroodspot, Rolf Daniel
Clostridium oryzae was originally isolated from the soil of a Japanese rice field. C. oryzae represents a novel species within the genus Clostridium and is associated with anaerobic rice straw degradation. Here, we present the draft genome sequence of C. oryzae DSM 28571 (5.076 Mbp), containing 4,590 predicted protein-coding genes.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28475028/chthonobacter-albigriseus-gen-nov-sp-nov-isolated-from-grass-field-soil
#12
Dohak Kim, Keunsoo Kang, Tae-Young Ahn
A novel aerobic, Gram-stain-negative and non-motile bacterial strain, designated ED7T, was isolated from grass-field soil in Cheonan, Korea. Strain ED7T utilized methanol and methylamine, but not formate, as carbon and energy sources. The strain was able to grow at 20-42 °C (optimum 30-35 °C), at pH 7.0-8.5 (optimum pH 7.5-8.0), and in the absence of NaCl. According to the similarities of the 16S rRNA gene sequences, strain ED7T was most closely related to the genera Labrenzia (≤93.3 % 16S rRNA gene sequence similarity), Pleomorphomonas (≤93...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28323300/a-walk-on-the-wild-side-oryza-species-as-source-for-rice-abiotic-stress-tolerance
#13
Paloma Koprovski Menguer, Raul Antonio Sperotto, Felipe Klein Ricachenevsky
Oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. Although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population increase are expected to soon call for new, fast generation of stress tolerant rice germplasm, and current within-species rice diversity might not be enough to overcome such needs. The Oryza genus contains other 23 wild species, with only Oryza glaberrima being also domesticated...
2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28303127/functional-characterization-of-endophytic-fungal-community-associated-with-oryza-sativa-l-and-zea-mays-l
#14
Momota Potshangbam, S Indira Devi, Dinabandhu Sahoo, Gary A Strobel
In a natural ecosystem, the plant is in a symbiotic relationship with beneficial endophytes contributing huge impact on its host plant. Therefore, exploring beneficial endophytes and understanding its interaction is a prospective area of research. The present work aims to characterize the fungal endophytic communities associated with healthy maize and rice plants and to study the deterministic factors influencing plant growth and biocontrol properties against phytopathogens, viz, Pythium ultimum, Sclerotium oryzae, Rhizoctonia solani, and Pyricularia oryzae...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28154946/rhizobium-rhizosphaerae-sp-nov-a-novel-species-isolated-from-rice-rhizosphere
#15
Juan-Juan Zhao, Jun Zhang, Rui-Jie Zhang, Cai-Wen Zhang, Hua-Qun Yin, Xiao-Xia Zhang
Two novel, Gram-negative, motile, rod-shaped, aerobic bacterial strains, MH17(T) and RD15, were isolated from the sterilized root and rhizosphere soil of rice, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that the similarity between strains MH17(T) and RD15 was 100%. The isolates exhibit high sequence similarities to Rhizobium oryzae CGMCC 1.7048(T) (98.7%) and Rhizobium petrolearium SL-1(T) (97.0% and 97.1%), which supports that they belong to a novel species in the genus Rhizobium...
May 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#16
Yanfei Huang, Jinglin Wang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Xinxin Lu
PURPOSE: Filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. Although matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS has been employed to identify various fungi, its efficacy in the identification of FRS fungi is less clear. METHODOLOGY: A total of 153 FRS isolates obtained from patients were analysed at the Clinical Laboratory at the Beijing Tongren Hospital affiliated to the Capital Medical University, between January 2014 and December 2015...
March 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27959784/rhizobium-oryziradicis-sp-nov-isolated-from-rice-roots
#17
Juan-Juan Zhao, Jun Zhang, Lei Sun, Rui-Jie Zhang, Cai-Wen Zhang, Hua-Qun Yin, Xiao-Xia Zhang
Two Gram-stain-negative, aerobic, rod-shaped endophytic bacterial strains, N19T and N11-2, were isolated from fresh rice (Oryza sativa) roots during investigation of the rice endophytic bacterial diversity. The 16S rRNA gene sequence results indicated that the similarity between strains N19T and N11-2 was 100 %. Both of them belong to the genus Rhizobium, with close similarity to Rhizobium taibaishanense CCNWSX 0483T (97.7 %), followed by Rhizobium vitis NCPPB 3554T (97.5 %). The sequence similarities of the housekeeping genes recA, gyrB and glnA between the novel isolates and members of the established species of the genus Rhizobium were less than 87 %...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902309/flexivirga-oryzae-sp-nov-isolated-from-soil-of-a-rice-paddy-and-emended-description-of-the-genus-flexivirga-anzai-et-al-2012
#18
Jong Woo Hyeon, Hye Rim Kim, Hyo Jung Lee, Sang Eun Jeong, Sang Ho Choi, Che Ok Jeon
A Gram-staining-positive, strictly aerobic bacterium, designated R1T, was isolated from a rice paddy in South Korea. Cells were non-motile cocci showing oxidase-negative and catalase-positive activities. Growth of strain R1T was observed at 10-37 °C (optimum, 30 °C) and pH 5.0-9.0 (optimum, pH 7.0). Strain R1T contained iso-C16 : 0, summed feature 9 (comprising iso-C17 : 1ω9c/10-methyl C16 : 0), anteiso-C17 : 0, iso-C17 : 0 and anteiso-C17 : 1ω9c as the major fatty acids and MK-8 (H4) and MK-8 (H6) as the isoprenoid quinones...
February 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902265/oharaeibacter-diazotrophicus-gen-nov-sp-nov-a-diazotrophic-and-facultatively-methylotrophic-bacterium-isolated-from-rice-rhizosphere
#19
Haoxin Lv, Sachiko Masuda, Yoshiko Fujitani, Nurettin Sahin, Akio Tani
A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T was Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30T was most closely related to Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30T showed its distinct phylogenetic position with respect to species with validly published names...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27889940/sequencing-of-australian-wild-rice-genomes-reveals-ancestral-relationships-with-domesticated-rice
#20
Marta Brozynska, Dario Copetti, Agnelo Furtado, Rod A Wing, Darren Crayn, Glen Fox, Ryuji Ishikawa, Robert J Henry
The related A genome species of the Oryza genus are the effective gene pool for rice. Here, we report draft genomes for two Australian wild A genome taxa: O. rufipogon-like population, referred to as Taxon A, and O. meridionalis-like population, referred to as Taxon B. These two taxa were sequenced and assembled by integration of short- and long-read next-generation sequencing (NGS) data to create a genomic platform for a wider rice gene pool. Here, we report that, despite the distinct chloroplast genome, the nuclear genome of the Australian Taxon A has a sequence that is much closer to that of domesticated rice (O...
June 2017: Plant Biotechnology Journal
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