Zhongqiang Qi, Fangyi Ju, Yunxia Guo, Yan Du, Junjie Yu, Rongsheng Zhang, Mina Yu, Huijuan Cao, Tianqiao Song, Xiayan Pan, Tingting Dai, Yongfeng Liu
Rice blast, caused by Pyricularia oryzae, is one of the most destructive rice diseases worldwide. Using resistant rice varieties is the most cost-effective way to control rice blast. Consequently, it is critical to monitor the distribution frequency of avirulence genes in rice planting field to facilitate the breedings of resistant rice varieties. In this study, we established a rapid RPA-LFD detection system for the identification of AvrPik, Avr-Piz-t and Avr-Pi9. The optimized reaction temperature and duration were 37°C and 20 min, indicating that the reaction system could be initiated by body temperature without relying on any precision instruments...
April 8, 2024: Plant Disease