keyword
https://read.qxmd.com/read/38464155/modular-vascularized-hypertrophic-cartilage-constructs-for-bone-tissue-engineering-applications
#21
Nicholas G Schott, Gurcharan Kaur, Rhima Coleman, Jan P Stegemann
Insufficient vascularization is a main barrier to creating engineered bone grafts for treating large and ischemic defects. Modular tissue engineering approaches have promise in this application because of the ability to combine tissue types and to localize microenvironmental cues to drive desired cell function. In direct bone formation approaches, it is challenging to maintain sustained osteogenic activity, since vasculogenic cues can inhibit tissue mineralization. This study harnessed the physiological process of endochondral ossification to create multiphase tissues that allowed concomitant mineralization and vessel formation...
March 1, 2024: bioRxiv
https://read.qxmd.com/read/38463824/cdkn1a-regulation-on-chondrogenic-differentiation-of-human-chondrocytes-in-osteoarthritis-through-single-cell-and-bulk-sequencing-analysis
#22
JOURNAL ARTICLE
Chao Fang, Shanbang Zhu, Rui Zhong, Gang Yu, Shuai Lu, Zhilin Liu, Jingyu Gao, Chengyuan Yan, Yingming Wang, Xinzhe Feng
OBJECTIVE: Chondrocyte death is the hallmark of cartilage degeneration during osteoarthritis (OA). However, the specific pathogenesis of cell death in OA chondrocytes has not been elucidated. This study aims to validate the role of CDKN1A, a key programmed cell death (PCD)-related gene, in chondrogenic differentiation using a combination of single-cell and bulk sequencing approaches. DESIGN: OA-related RNA-seq data (GSE114007, GSE55235, GSE152805) were downloaded from Gene Expression Omnibus database...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38451090/nanomedicines-promote-cartilage-regeneration-in-osteoarthritis-by-synergistically-enhancing-chondrogenesis-of-mesenchymal-stem-cells-and-regulating-inflammatory-environment
#23
JOURNAL ARTICLE
Shi-He Cui, Yi Yan, An Lu, Yun Dou, Zhen-Wen Li, Ze-Hang Zhu, Ming-Ze Du, Yue-Feng Zhu, Xin Chen, Xiangyu Wang, Lin-Xia Jiang, Yujie Shi, Xiaoyan Liu, Yuan-Jun Zhu, Dong Jiang, Jian-Cheng Wang
Osteoarthritis (OA) is a degenerative joint disease characterized by progressive erosion of the articular cartilage and inflammation. Mesenchymal stem cells' (MSCs) transplantation in OA treatment is emerging, but its clinical application is still limited by the low efficiency in oriented differentiation. In our study, to improve the therapeutic efficiencies of MSCs in OA treatment by carbonic anhydrase IX (CA9) siRNA (siCA9)-based inflammation regulation and Kartogenin (KGN)-based chondrogenic differentiation, the combination strategy of MSCs and the nanomedicine codelivering KGN and siCA9 (AHK-CaP/siCA9 NPs) was used...
March 7, 2024: ACS Nano
https://read.qxmd.com/read/38444516/effects-of-self-assembled-type-ii-collagen-fibrils-on-the-morphology-and-growth-of-pre-chondrogenic-atdc5-cells
#24
JOURNAL ARTICLE
Linyan Shi, Kazuhiro Ura, Yasuaki Takagi
OBJECTIVE: Although type II collagen could have marked potential for developing cartilage tissue engineering (CTE) scaffolds, its erratic supply and viscous nature have limited these studies, and there are no studies on the use of marine-derived type II collagen fibrils for CTE scaffold materials. In this study, we aimed to generate a fibril-based, thin-layered scaffold from marine-derived type II collagen and investigate its chondrogenic potential. METHODS: Time-lapse observations revealed the cell adhesion process...
June 2024: Osteoarthritis and cartilage open
https://read.qxmd.com/read/38440852/increased-wnt5a-ror2-signaling-is-associated-with-chondrogenesis-in-meniscal-degeneration
#25
JOURNAL ARTICLE
Yusuke Inoue, Ken Kumagai, Kimi Ishikawa, Ikuma Kato, Youhei Kusaba, Takuma Naka, Kiyotaka Nagashima, Hyonmin Choe, Hiroyuki Ike, Naomi Kobayashi, Yutaka Inaba
The aim of the present study was to investigate the association between chondrogenic differentiation and Wnt signal expression in the degenerative process of the human meniscus. Menisci were obtained from patients with and without knee osteoarthritis (OA), and degeneration was histologically assessed using a grading system. Immunohistochemistry, real-time polymerase chain reaction (PCR), and Western blot analysis were performed to examine the expressions of chondrogenic markers and of the components of Wnt signaling...
March 5, 2024: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://read.qxmd.com/read/38439927/cd62e-and-ros-responsive-ets-improves-cartilage-repair-by-inhibiting-endothelial-cell-activation-through-opa1-mediated-mitochondrial-homeostasis
#26
JOURNAL ARTICLE
Pengcheng Tu, Yalan Pan, Lining Wang, Bin Li, Xiaoxian Sun, Zhongqing Liang, Mengmin Liu, Zitong Zhao, Chengjie Wu, Jianwei Wang, Zhifang Wang, Yu Song, Yafeng Zhang, Yong Ma, Yang Guo
Background: In the environment of cartilage injury, the activation of vascular endothelial cell (VEC), marked with excessive CD62E and reactive oxygen species (ROS), can affect the formation of hyaluronic cartilage. Therefore, we developed a CD62E- and ROS-responsive drug delivery system using E-selectin binding peptide, Thioketal, and silk fibroin (ETS) to achieve targeted delivery and controlled release of Clematis triterpenoid saponins (CS) against activated VEC, and thus promote cartilage regeneration. Methods: We prepared and characterized ETS/CS and verified their CD62E- and ROS-responsive properties in vitro...
2024: Biomaterials Research
https://read.qxmd.com/read/38433653/evaluation-of-rabbit-adipose-derived-stem-cells-fate-in-perfused-multilayered-silk-fibroin-composite-scaffold-for-osteochondral-repair
#27
JOURNAL ARTICLE
Sara Zadegan, Bahman Vahidi, Jhamak Nourmohammadi, Asiyeh Shojaee, Nooshin Haghighipour
Development of osteochondral tissue engineering approaches using scaffolds seeded with stem cells in association with mechanical stimulations has been recently considered as a promising technique for the repair of this tissue. In this study, an integrated and biomimetic trilayered silk fibroin (SF) scaffold containing SF nanofibers in each layer was fabricated. The osteogenesis and chondrogenesis of stem cells seeded on the fabricated scaffolds were investigated under a perfusion flow. 3-Dimethylthiazol-2,5-diphenyltetrazolium bromide assay showed that the perfusion flow significantly enhanced cell viability and proliferation...
March 2024: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://read.qxmd.com/read/38433390/regenerative-capacity-of-human-pluripotent-stem-cell-derived-articular-chondrocytes-in-vitro
#28
JOURNAL ARTICLE
Rosanne M Raftery, Steven K Pregizer, Sophia Kocher, April M Craft
The ideal cell source for articular cartilage repair remains elusive. Using developmentally inspired differentiation protocols, we induced human pluripotent stem cells (hPSCs) toward articular chondrocytes capable of joint cartilage repair in rodent models, which were distinct from growth plate chondrocytes, fated to be replaced by bone in vivo. Working toward clinical translation, we demonstrated controlled differentiation into chondrocytes by comprehensive gene expression analysis at each step of the differentiation...
March 3, 2024: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://read.qxmd.com/read/38432161/investigation-of-the-differentiation-potential-of-pericyte-cells-as-an-alternative-source-of-mesenchymal-stem-cells
#29
JOURNAL ARTICLE
Selen Polat, Yusufhan Yazir, Gökhan Duruksu, Kamil Can Kiliç, Serap Mert, Gülçin Gacar, Büşra Öncel Duman, Zehra Seda Halbutoğullari
BACKGROUND: The mesenchymal stem cells (MSCs) with characterized by their multipotency and capacity to differentiate into various tissue cell types, have led to their incorporation in regenerative medicine research. However, the limited numbers of MSCs in the human body and their diverse differentiation capabilities in tissues highlight the need for exploring alternative regenerative cell sources. In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs)...
March 2, 2024: Acta Histochemica
https://read.qxmd.com/read/38420649/characterization-of-acellular-cartilage-matrix-sodium-alginate-scaffolds-in-various-proportions
#30
JOURNAL ARTICLE
Wang Lu, Mengchu Yang, Yanan Zhang, Baoxi Meng, Fulian Ma, Wanjun Wang, Teng Guo
The development of 3D bioprinting technology has provided a new solution to address the shortage of donors, multiple surgeries, and aesthetic concerns in microtia reconstruction surgery. The production of bio-inks is the most critical aspect of 3D bioprinting. Acellular cartilage matrix (ACM) and sodium alginate (SA) are commonly used 3D bioprinting materials, and there have been reports of their combined use. However, there is a lack of comprehensive evaluations on ACM-SA scaffolds with different proportions...
February 29, 2024: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/38419462/passaged-articular-chondrocytes-from-the-superficial-zone-and-deep-zone-can-regain-zone-specific-properties-after-redifferentiation
#31
JOURNAL ARTICLE
Elizabeth E R Davis, Thomas J Manzoni, Vanessa J Bianchi, Joanna F Weber, Po Han Wu, Suresh C Regmi, Stephen D Waldman, Tannin A Schmidt, Alvin W Su, Rita A Kandel, Justin Parreno
BACKGROUND: Bioengineered cartilage is a developing therapeutic to repair cartilage defects. The matrix must be rich in collagen type II and aggrecan and mechanically competent, withstanding compressive and shearing loads. Biomechanical properties in native articular cartilage depend on the zonal architecture consisting of 3 zones: superficial, middle, and deep. The superficial zone chondrocytes produce lubricating proteoglycan-4, whereas the deep zone chondrocytes produce collagen type X, which allows for integration into the subchondral bone...
February 28, 2024: American Journal of Sports Medicine
https://read.qxmd.com/read/38414413/graphene-oxide-activates-canonical-tgf%C3%AE-signalling-in-a-human-chondrocyte-cell-line-via-increased-plasma-membrane-tension
#32
JOURNAL ARTICLE
Leona Ogene, Steven Woods, Joseph Hetmanski, Neus Lozano, Angeliki Karakasidi, Patrick T Caswell, Kostas Kostarelos, Marco A N Domingos, Sandra Vranic, Susan J Kimber
Graphene Oxide (GO) has been shown to increase the expression of key cartilage genes and matrix components within 3D scaffolds. Understanding the mechanisms behind the chondroinductive ability of GO is critical for developing articular cartilage regeneration therapies but remains poorly understood. The objectives of this work were to elucidate the effects of GO on the key chondrogenic signalling pathway - TGFβ and identify the mechanism through which signal activation is achieved in human chondrocytes...
February 28, 2024: Nanoscale
https://read.qxmd.com/read/38413962/dnmt3b-ablation-affects-fracture-repair-process-by-regulating-apoptosis
#33
JOURNAL ARTICLE
Xu Wang, Qinwen Ge, Qinghe Zeng, Kaiao Zou, Zhengsheng Bao, Jun Ying, Zhen Wu, Hongting Jin, Jiali Chen, Taotao Xu
PURPOSE: Previous studies have shown that DNA methyltransferase 3b (Dnmt3b) is the only Dnmt responsive to fracture repair and Dnmt3b ablation in Prx1-positive stem cells and chondrocyte cells both delayed fracture repair. Our study aims to explore the influence of Dnmt3b ablation in Gli1-positive stem cells in fracture healing mice and the underlying mechanism. METHODS: We generated Gli1-CreERT2; Dnmt3bflox/flox (Dnmt3bGli1ER ) mice to operated tibia fracture. Fracture callus tissues of Dnmt3bGli1ER mice and control mice were collected and analyzed by X-ray, micro-CT, biomechanical testing, histopathology and TUNEL assay...
February 27, 2024: BMC Musculoskeletal Disorders
https://read.qxmd.com/read/38410163/one-step-stromal-vascular-fraction-therapy-in-osteoarthritis-with-tropoelastin-enhanced-autologous-stromal-vascular-fraction-gel
#34
JOURNAL ARTICLE
Junjun Yang, Xin Wang, XueBao Zeng, Rong Wang, Yanming Ma, Zhenlan Fu, Zu Wan, Zhi Wang, Liu Yang, Guangxing Chen, Xiaoyuan Gong
Background: Osteoarthritis (OA) is a debilitating degenerative joint disease, leading to significant pain and disability. Despite advancements, current regenerative therapies, such as mesenchymal stem cells (MSCs), face challenges in clinical efficacy and ethical considerations. This study aimed to evaluate the therapeutic potential of stromal vascular fraction gel (SVF-gel) in comparison to available treatments like hyaluronic acid (HA) and adipose-derived stem cells (ADSCs) and to assess the enhancement of this potential by incorporating tropoelastin (TE)...
2024: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/38409269/gli1-labels-progenitors-during-chondrogenesis-in-postnatal-mice
#35
JOURNAL ARTICLE
Boer Li, Puying Yang, Fangyuan Shen, Chengjia You, Fanzi Wu, Yu Shi, Ling Ye
Skeletal growth promoted by endochondral ossification is tightly coordinated by self-renewal and differentiation of chondrogenic progenitors. Emerging evidence has shown that multiple skeletal stem cells (SSCs) participate in cartilage formation. However, as yet, no study has reported the existence of common long-lasting chondrogenic progenitors in various types of cartilage. Here, we identify Gli1+ chondrogenic progenitors (Gli1+ CPs), which are distinct from PTHrP+ or FoxA2+ SSCs, are responsible for the lifelong generation of chondrocytes in the growth plate, vertebrae, ribs, and other cartilage...
February 26, 2024: EMBO Reports
https://read.qxmd.com/read/38407380/a-synthetic-closed-looped-gene-circuit-for-the-autonomous-regulation-of-runx2-activity-during-chondrogenesis
#36
JOURNAL ARTICLE
Gurcharan Kaur, Biming Wu, Sanjana Murali, Thomas Lanigan, Rhima M Coleman
The transcription factor RUNX2 is a key regulator of chondrocyte phenotype during development, making it an ideal target for prevention of undesirable chondrocyte maturation in cartilage tissue-engineering strategies. Here, we engineered an autoregulatory gene circuit (cisCXp-shRunx2) that negatively controls RUNX2 activity in chondrogenic cells via RNA interference initiated by a tunable synthetic Col10a1-like promoter (cisCXp). The cisCXp-shRunx2 gene circuit is designed based on the observation that induced RUNX2 silencing after early chondrogenesis enhances the accumulation of cartilaginous matrix in ATDC5 cells...
February 29, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38397915/peptide-based-biomaterials-for-bone-and-cartilage-regeneration
#37
REVIEW
Kausik Kapat, Sakshi Kumbhakarn, Rahul Sable, Prashil Gondane, Shruti Takle, Pritiprasanna Maity
The healing of osteochondral defects (OCDs) that result from injury, osteochondritis, or osteoarthritis and bear lesions in the cartilage and bone, pain, and loss of joint function in middle- and old-age individuals presents challenges to clinical practitioners because of non-regenerative cartilage and the limitations of current therapies. Bioactive peptide-based osteochondral (OC) tissue regeneration is becoming more popular because it does not have the immunogenicity, misfolding, or denaturation problems associated with original proteins...
January 29, 2024: Biomedicines
https://read.qxmd.com/read/38396967/identification-of-the-reference-genes-for-relative-qrt-pcr-assay-in-two-experimental-models-of-rabbit-and-horse-subcutaneous-ascs
#38
JOURNAL ARTICLE
Zhenya Ivanova, Valeria Petrova, Natalia Grigorova, Ekaterina Vachkova
Obtaining accurate and reliable gene expression results in real-time RT-PCR (qRT-PCR) data analysis requires appropriate normalization by carefully selected reference genes, either a single or a combination of multiple housekeeping genes (HKGs). The optimal reference gene/s for normalization should demonstrate stable expression across varying conditions to diminish potential influences on the results. Despite the extensive database available, research data are lacking regarding the most appropriate HKGs for qRT-PCR data analysis in rabbit and horse adipose-derived stem cells (ASCs)...
February 14, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38395992/piezo1-expression-in-chondrocytes-controls-endochondral-ossification-and-osteoarthritis-development
#39
JOURNAL ARTICLE
Laura J Brylka, Assil-Ramin Alimy, Miriam E A Tschaffon-Müller, Shan Jiang, Tobias Malte Ballhause, Anke Baranowsky, Simon von Kroge, Julian Delsmann, Eva Pawlus, Kian Eghbalian, Klaus Püschel, Astrid Schoppa, Melanie Haffner-Luntzer, David J Beech, Frank Timo Beil, Michael Amling, Johannes Keller, Anita Ignatius, Timur A Yorgan, Tim Rolvien, Thorsten Schinke
Piezo proteins are mechanically activated ion channels, which are required for mechanosensing functions in a variety of cell types. While we and others have previously demonstrated that the expression of Piezo1 in osteoblast lineage cells is essential for bone-anabolic processes, there was only suggestive evidence indicating a role of Piezo1 and/or Piezo2 in cartilage. Here we addressed the question if and how chondrocyte expression of the mechanosensitive proteins Piezo1 or Piezo2 controls physiological endochondral ossification and pathological osteoarthritis (OA) development...
February 23, 2024: Bone Research
https://read.qxmd.com/read/38395283/fabrication-of-stem-cell-heterospheroids-with-sustained-release-chitosan-and-poly-lactic-co-glycolic-acid-microspheres-to-guide-cell-fate-toward-chondrogenic-differentiation
#40
JOURNAL ARTICLE
Tiep Tien Nguyen, Yun-Seo Kil, Jong-Hyuk Sung, Yu Seok Youn, Ji Hoon Jeong, Jung Heon Lee, Hu-Lin Jiang, Simmyung Yook, Joo-Won Nam, Jee-Heon Jeong
Mesenchymal stem cell (MSC)-based therapies show great potential in treating various diseases. However, control of the fate of injected cells needs to be improved. In this work, we developed an efficient methodology for modulating chondrogenic differentiation of MSCs. We fabricated heterospheroids with two sustained-release depots, a quaternized chitosan microsphere (QCS-MP) and a poly (lactic-co-glycolic acid) microsphere (PLGA-MP). The results show that heterospheroids composed of 1 × 104 to 5 × 104 MSCs formed rapidly during incubation in methylcellulose medium and maintained high cell viability in long-term culture...
February 21, 2024: International Journal of Biological Macromolecules
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