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https://www.readbyqxmd.com/read/28813497/high-hydrostatic-pressure-induces-pro-osteoarthritic-changes-in-cartilage-precursor-cells-a-transcriptome-analysis
#1
Kevin Montagne, Yasuko Onuma, Yuzuru Ito, Yasuhiko Aiki, Katsuko S Furukawa, Takashi Ushida
Due to the high water content of cartilage, hydrostatic pressure is likely one of the main physical stimuli sensed by chondrocytes. Whereas, in the physiological range (0 to around 10 MPa), hydrostatic pressure exerts mostly pro-chondrogenic effects in chondrocyte models, excessive pressures have been reported to induce detrimental effects on cartilage, such as increased apoptosis and inflammation, and decreased cartilage marker expression. Though some genes modulated by high pressure have been identified, the effects of high pressure on the global gene expression pattern have still not been investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28811164/corrigendum-to-functional-quantum-dot-sirna-nanoplexes-to-regulate-chondrogenic-differentiation-of-mesenchymal-stem-cells-acta-biomater-46-2016-165-176
#2
Yang Wu, Bo Zhou, Fuben Xu, Xiaoyong Wang, Gang Liu, Li Zheng, Jinmin Zhao, Xingdong Zhang
No abstract text is available yet for this article.
August 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28810613/mir-127-5p-promotes-chondrogenic-differentiation-in-rat-bone-marrow-mesenchymal-stem-cells
#3
Zhaolong Xue, Yanli Meng, Jianhua Ge
The effect and related mechanisms of miR-127-5p on the cartilage differentiation of rat bone marrow mesenchymal stem cells (BMSCs) was investigated. Rat BMSCs were generated and transfected with miR-127-5p, RT-PCR and Safranin O staining were used to detect the effect of miR-127-5p on the cartilage differentiation of rat BMSCs. Western blot analysis was used to detect the related mechanisms of miR-127-5p on the cartilage differentiation of rat BMSCs. Genes related to cartilage differentiation such as Sox9, collagen II and aggrecan were significantly increased in the group which were transfected with miR-127-5p, while collagen X, which was related to cartilage hypertrophy, was decreased in the miR-127-5p transfected group...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810001/deferoxamine-synergizes-with-transforming-growth-factor-%C3%AE-signaling-in-chondrogenesis
#4
Zheng Huang, Guangxu He, Yanke Huang
Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, is an epidemic disease that affects millions of people worldwide. Despite extensive recent work on the cellular biology of osteoarthritis, the precise mechanisms involved are still poorly understood and there is no effective treatment for this disease. The role of transforming growth factor-beta (TGF-β) in promoting chondrogenesis and inducing the expression of cartilage-specific extracellular matrix molecules to form cartilage is well-established...
August 14, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28807903/transgelin-expressing-myofibroblasts-orchestrate-ventral-midline-closure-through-tgf-%C3%AE-signalling
#5
Bashar Aldeiri, Urmas Roostalu, Alessandra Albertini, Jason Wong, Antonino Morabito, Giulio Cossu
Ventral body wall (VBW) defects are among the most common congenital malformations, yet their embryonic origin and underlying molecular mechanisms have remained poorly characterized. Although transforming growth factor beta (TGF-β) signalling is essential for VBW closure the responding cells are not known. Here we identify a population of migratory myofibroblasts at the leading edge of the closing VBW that express the actin-binding protein Transgelin (TAGLN) and TGF-β receptor (TGFβR). These cells respond to a temporally regulated TGF-β2 gradient originating from the epithelium of the primary body wall...
August 14, 2017: Development
https://www.readbyqxmd.com/read/28807899/pitx1-directly-modulates-the-core-limb-development-program-to-implement-hindlimb-identity
#6
Stephen Nemec, Maëva Luxey, Deepak Jain, Aurélie Huang Sung, Tomi Pastinen, Jacques Drouin
Forelimbs (FL) and hindlimbs (HL) develop complex musculoskeletal structures that rely on the deployment of a conserved developmental program. Pitx1, a transcription factor gene with expression restricted to HL and absent from FL, plays an important role in generating HL features. The genomic mechanisms by which Pitx1 effects HL identity remain poorly understood, however. Here, we use expression profiling and analysis of direct Pitx1 targets to characterize the HL- and FL-restricted genetic programs and situate the Pitx1-dependent gene network within the context of limb-specific gene regulation...
August 14, 2017: Development
https://www.readbyqxmd.com/read/28782836/histone-deacetylase-3-deletion-in-mesenchymal-progenitor-cells-hinders-long-bone-development
#7
Marina Feigenson, Lomeli Carpio Shull, Earnest L Taylor, Emily T Camilleri, Scott M Reister, Andre J van Wijnen, Elizabeth W Bradley, Jennifer J Westendorf
Long bone formation is a complex process that requires precise transcriptional control of gene expression programs in mesenchymal progenitor cells. Histone deacetylases (Hdacs) coordinate chromatin structure and gene expression by enzymatically removing acetyl groups from histones and other proteins. Hdac inhibitors are used clinically to manage mood disorders, cancers and other conditions, but are teratogenic to the developing skeleton and increase fracture risk in adults. In this study, the functions of Hdac3, one of the enzymes blocked by current Hdac inhibitor therapies, in skeletal mesenchymal progenitor cells were determined...
August 7, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28772498/3d-printing-of-cytocompatible-water-based-light-cured-polyurethane-with-hyaluronic-acid-for-cartilage-tissue-engineering-applications
#8
Ming-You Shie, Wen-Ching Chang, Li-Ju Wei, Yu-Hsin Huang, Chien-Han Chen, Cheng-Ting Shih, Yi-Wen Chen, Yu-Fang Shen
Diseases in articular cartilages have affected millions of people globally. Although the biochemical and cellular composition of articular cartilages is relatively simple, there is a limitation in the self-repair ability of the cartilage. Therefore, developing strategies for cartilage repair is very important. Here, we report on a new liquid resin preparation process of water-based polyurethane based photosensitive materials with hyaluronic acid with application of the materials for 3D printed customized cartilage scaffolds...
February 8, 2017: Materials
https://www.readbyqxmd.com/read/28770354/thrap3-interacts-with-and-inhibits-the-transcriptional-activity-of-sox9-during-chondrogenesis
#9
Takashi Sono, Haruhiko Akiyama, Shigenori Miura, Jian Min Deng, Chisa Shukunami, Yuji Hiraki, Yu Tsushima, Yoshiaki Azuma, Richard R Behringer, Shuichi Matsuda
Sex-determining region Y (Sry)-box (Sox)9 is required for chondrogenesis as a transcriptional activator of genes related to chondrocyte proliferation, differentiation, and cartilage-specific extracellular matrix. Although there have been studies investigating the Sox9-dependent transcriptional complexes, not all their components have been identified. In the present study, we demonstrated that thyroid hormone receptor-associated protein (THRAP)3 is a component of a SOX9 transcriptional complex by liquid chromatography mass spectrometric analysis of FLAG-tagged Sox9-binding proteins purified from FLAG-HA-tagged Sox9 knock-in mice...
August 2, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/28769981/human-suprapatellar-fat-pad-derived-mesenchymal-stem-cells-induce-chondrogenesis-and-cartilage-repair-in-a-model-of-severe-osteoarthritis
#10
Ignacio Muñoz-Criado, Jose Meseguer-Ripolles, Maravillas Mellado-López, Ana Alastrue-Agudo, Richard J Griffeth, Jerónimo Forteza-Vila, Ramón Cugat, Montserrat García, Victoria Moreno-Manzano
Cartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown to restore degenerated cartilage; however, regenerative differences of ASC would depend on the source of adipose tissue. The infra- and suprapatellar fat pads surrounding the knee offer a potential autologous source of ASC for patients after complete joint substitution...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28768930/curcumin-inhibits-chondrocyte-hypertrophy-of-mesenchymal-stem-cells-through-ihh-and-notch-signaling-pathways
#11
Zhen Cao, Ce Dou, Shiwu Dong
Using tissue engineering technique to repair cartilage damage caused by osteoarthritis is a promising strategy. However, the regenerated tissue usually is fibrous cartilage, which has poor mechanical characteristics compared to hyaline cartilage. Chondrocyte hypertrophy plays an important role in this process. Thus, it is very important to find out a suitable way to maintain the phenotype of chondrocytes and inhibit chondrocyte hypertrophy. Curcumin deriving from turmeric was reported with anti-inflammatory and anti-tumor pharmacological effects...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28768899/aberrant-perichondrial-bmp-signaling-mediates-multiple-osteochondromagenesis-in-mice
#12
Toshihiro Inubushi, Satoshi Nozawa, Kazu Matsumoto, Fumitoshi Irie, Yu Yamaguchi
Multiple hereditary exostoses (MHE) is characterized by the development of numerous benign bony tumors (osteochondromas). Although it has been well established that MHE is caused by mutations in EXT1 and EXT2, which encode glycosyltransferase essential for heparan sulfate (HS) biosynthesis, the cellular origin and molecular mechanisms of MHE remain elusive. Here, we show that in Ext1 mutant mice, osteochondromas develop from mesenchymal stem cell-like progenitor cells residing in the perichondrium, and we show that enhanced BMP signaling in these cells is the primary signaling defect that leads to osteochondromagenesis...
August 3, 2017: JCI Insight
https://www.readbyqxmd.com/read/28767737/tissue-engineering-potential-of-human-dermis-isolated-adult-stem-cells-from-multiple-anatomical-locations
#13
Heenam Kwon, Anne K Haudenschild, Wendy E Brown, Natalia Vapniarsky, Nikolaos K Paschos, Boaz Arzi, Jerry C Hu, Kyriacos A Athanasiou
Abundance and accessibility render skin-derived stem cells an attractive cell source for tissue engineering applications. Toward assessing their utility, the variability of constructs engineered from human dermis-isolated adult stem (hDIAS) cells was examined with respect to different anatomical locations (foreskin, breast, and abdominal skin), both in vitro and in a subcutaneous, athymic mouse model. All anatomical locations yielded hDIAS cells with multi-lineage differentiation potentials, though adipogenesis was not seen for foreskin-derived hDIAS cells...
2017: PloS One
https://www.readbyqxmd.com/read/28765636/high-resolution-patterned-cellular-constructs-by-droplet-based-3d-printing
#14
Alexander D Graham, Sam N Olof, Madeline J Burke, James P K Armstrong, Ellina A Mikhailova, James G Nicholson, Stuart J Box, Francis G Szele, Adam W Perriman, Hagan Bayley
Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (10(7) cells mL(-1)) and a high droplet resolution of 1 nL...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28764769/anabolic-role-of-lysyl-oxidase-like-2-in-cartilage-of-knee-and-temporomandibular-joints-with-osteoarthritis
#15
Weam Alshenibr, Mustafa M Tashkandi, Saqer F Alsaqer, Yazeed Alkheriji, Amelia Wise, Sadanand Fulzele, Pushkar Mehra, Mary B Goldring, Louis C Gerstenfeld, Manish V Bais
BACKGROUND: Lysyl oxidase like-2 (LOXL2) is a copper-dependent amine oxidase. Our previous studies showed that LOXL2 is elevated during mouse fracture healing. The goal of this study was to evaluate the potential of LOXL2 to act as an anabolic agent in cartilage affected by osteoarthritis (OA). METHODS: LOXL2 was visualized in tissues from human knee and hip joints and temporomandibular joints (TMJ) by immunofluorescence. The activity of LOXL2 in human articular and TMJ chondrocytes was assessed by cell-based assays, microarray analysis, and RT-qPCR, and LOXL2-mediated activation of NF-κB and extracellular signal-related kinase (ERK) signaling pathways was measured by western blotting...
August 2, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28763577/bioreactors-with-hydrostatic-pressures-imitating-physiological-environments-in-intervertebral-discs
#16
REVIEW
Jovana Zvicer, Bojana Obradovic
Intervertebral discs are normally exposed to variety of loads and stresses but hydrostatic pressure (HP) could be the main biosignal for chondrogenic cell differentiation and maintenance of this tissue. Although there are simple approaches to intermittently expose cell cultures to HP in separate material testing devices, utilization of biomimetic bioreactors aiming to provide in vitro conditions mimicking those found in vivo, attracts special attention. However, design of such bioreactors is complex due to the requirement of high HP magnitudes (up to 3 MPa) applied in different regimes mimicking pressures arising in IVD during normal daily activities...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28763118/mechanical-stimulation-of-mesenchymal-stem-cells-implications-for-cartilage-tissue-engineering
#17
REVIEW
Niamh Fahy, Mauro Alini, Martin J Stoddart
Articular cartilage is a load-bearing tissue playing a crucial mechanical role in diarthrodial joints, facilitating joint articulation, and minimizing wear. The significance of biomechanical stimuli in the development of cartilage and maintenance of chondrocyte phenotype in adult tissues has been well documented. Furthermore, dysregulated loading is associated with cartilage pathology highlighting the importance of mechanical cues in cartilage homeostasis. The repair of damaged articular cartilage resulting from trauma or degenerative joint disease poses a major challenge due to a low intrinsic capacity of cartilage for self-renewal, attributable to its avascular nature...
August 1, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28756737/mimicking-the-biochemical-and-mechanical-extracellular-environment-of-the-endochondral-ossification-process-to-enhance-the-in-vitro-mineralization-potential-of-human-mscs
#18
Fiona E Freeman, Jessica Schiavi, Meadhbh A Brennan, Peter Owens, Pierre Layrolle, Laoise McNamara
Chondrogenesis and mechanical stimulation of the cartilage template, are essential for bone formation via the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis...
July 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28749737/improved-chondrogenic-potential-and-proteomic-phenotype-of-porcine-chondrocytes-grown-in-optimized-culture-conditions
#19
Sehwon Koh, Molly Purser, Richard Wysk, Jorge A Piedrahita
For successful cartilage tissue engineering, the ability to generate a high number of chondrocytes in vitro while avoiding terminal differentiation or de-differentiation is critical. The ability to accomplish this by using the abundant and easily sampled costal cartilage could provide a practical alternative to the use of articular cartilage and mesenchymal stem cells. Chondrocytes isolated from pig costal cartilage were expanded in either serum-free medium with FGF2 (SFM) or fetal bovine serum-containing medium (SCM), under either high (21%) or low (5%) oxygen conditions...
August 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28747655/strategies-to-mitigate-variability-in-engineering-human-nasal-cartilage
#20
Stephen H J Andrews, Melanie Kunze, Aillette Mulet-Sierra, Lynn Williams, Khalid Ansari, Martin Osswald, Adetola B Adesida
Skin cancer and its associated treitments can have devastating consequences for survivors; this is particularly true when cancer occurs on the nose. Recent work has applied cell-based tissue engineering (TE) strategies to develop nasal cartilage constructs for reconstruction of the nose. In this study, we have generated human nasal cartilage on a clinically approved collagen scaffold to investigate the donor-to-donor variability of TE cartilage and evaluated strategies to mitigate it. We also evaluated the gene expression of the family of fibroblast growth factor receptors (FGFR1-4) and their association with tissue quality...
July 26, 2017: Scientific Reports
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