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https://www.readbyqxmd.com/read/28636254/pirfenidone-reduces-subchondral-bone-loss-and-fibrosis-after-murine-knee-cartilage-injury
#1
Deva D Chan, Jun Li, Wei Luo, Dan N Predescu, Brian J Cole, Anna Plaas
Pirfenidone is an anti-inflammatory and anti-fibrotic drug that has shown efficacy in lung and kidney fibrosis. Because inflammation and fibrosis have been linked to the progression of osteoarthritis, we investigated the effects of oral Pirfenidone in a mouse model of cartilage injury, which results in chronic inflammation and joint-wide fibrosis in mice that lack hyaluronan synthase 1 (Has1(-/-) ) in comparison to wild-type. Femoral cartilage was surgically injured in wild-type and Has1(-/-) mice, and Pirfenidone was administered in food starting after 3 days...
June 21, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28634835/impact-of-mechanical-stimulation-on-the-chondrogenic-processes-in-human-bone-marrow-aspirates-modified-to-overexpress-sox9-via-raav-vectors
#2
Jagadeesh K Venkatesan, Janina Frisch, Ana Rey-Rico, Gertrud Schmitt, Henning Madry, Magali Cucchiarini
BACKGROUND: Evaluation of gene-based approaches to target human bone marrow aspirates in conditions of mechanical stimulation that aim at reproducing the natural joint environment may allow to develop improved treatments for articular cartilage injuries. In the present study, we investigated the potential of rAAV-mediated sox9 gene transfer to enhance the chondrogenic differentiation processes in human bone marrow aspirates under established hydrodynamic conditions compared with the more commonly employed static culture conditions...
December 2017: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/28629066/glucosamine-modified-polyethylene-glycol-hydrogel-mediated-chondrogenic-differentiation-of-human-mesenchymal-stem-cells
#3
Hang Yao, Jingchen Xue, Qunfang Wang, Renjian Xie, Weichang Li, Sa Liu, Jinglei Cai, Dajiang Qin, Dong-An Wang, Li Ren
Glucosamine (GA) is an important cartilage matrix precursor for the glycosaminoglycan biochemical synthesis, and has positive effects on cartilage regeneration, particularly in osteoarthritis therapy. However, it has not been used as a bioactive group in scaffolds for cartilage repair widely. In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629047/two-phase-electrospinning-to-incorporate-growth-factors-loaded-chitosan-nanoparticles-into-electrospun-fibrous-scaffolds-for-bioactivity-retention-and-cartilage-regeneration
#4
Chongyang Wang, Wenxiu Hou, Xuran Guo, Juehong Li, Tu Hu, Manle Qiu, Shen Liu, Xiumei Mo, Xudong Liu
Growth factor is an essential ingredient to regulate mesenchymal stem cells (MSCs) chondrogenic differentiation in cartilage tissue engineering. However, non-osteochondral specification, short plasma half time and bioactivity loss restrict growth factor's application. Thus, novel chondrogenic growth factors, specifically target osteochondral lineage cells, that can be sustained release and bioactivity protected to exert functions continually and effectively have attracted increasing researchers' interest. To achieve these goals, chitosan nanoparticles and electrospun fiber scaffolds were used as dual release system to sustain release Nel-like molecule-1 (Nell-1) growth factor and protect bioactivity, then the effect and mechanism of Nell-1 on inducing human bone MSCs (hBMSCs) differentiate toward chondrocytes were investigated...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627746/hepatic-perivascular-mesenchymal-stem-cells-with-myogenic-properties
#5
Sudheer Shenoy P, Bipasha Bose
Pericytes are multipotent mesenchymal stem cells (MSCs) located on the walls of blood vessels in various organs and are characterized as CD146(+) cells.. In this study, we have first immunohistochemically detected such pericytes in the perivascular regions of liver from two mouse genotypes namely wild-type (WT) and myostatin null (Mstn(-/-) ). We further isolated such pericytes using sorting as CD146(+) CD34(-) CD56(-) CD45(-) cells. The main finding of this study involve the contrasting -fibrogenic versus myogenic behavior of liver pericytes from WTMstn(-/-) ) mouse respectively...
June 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28627740/biofabrication-of-soft-tissue-templates-for-engineering-the-bone-ligament-interface
#6
Ella Harris, Yurong Liu, Grainne Cuniffe, David Morrissey, Simon Carroll, Kevin Mulhall, Daniel J Kelly
Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition and function of the native enthesis. In the ligament-to-bone interface this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradation in tissue types facilitates the transfer of load between soft and hard structures while minimizing stress concentrations at the interface. Previous attempts to engineer the ligament-bone interface have utilized various scaffold materials with an array of various cell types and/or biological cues...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28625871/mir-27-regulates-chondrogenesis-by-suppressing-focal-adhesion-kinase-during-pharyngeal-arch-development
#7
Nergis Kara, Chunyao Wei, Alexander C Commanday, James G Patton
Cranial neural crest cells are a multipotent cell population that generate all the elements of the pharyngeal cartilage with differentiation into chondrocytes tightly regulated by temporal intracellular and extracellular cues. Here, we demonstrate a novel role for miR-27, a highly enriched microRNA in the pharyngeal arches, as a positive regulator of chondrogenesis. Knock down of miR-27 led to nearly complete loss of pharyngeal cartilage by attenuating proliferation and blocking differentiation of pre-chondrogenic cells...
June 15, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28622746/sol-gel-derived-lithium-releasing-glass-for-cartilage-regeneration
#8
Siwei Li, Anthony Lb Maçon, Manon Jacquemin, Molly M Stevens, Julian R Jones
Wnt-signalling cascade is one of the crucial pathways involved in the development and homeostasis of cartilage. Influencing this pathway can potentially contribute to improved cartilage repair or regeneration. One key molecular regulator of the Wnt pathway is the glycogen synthase kinase-3 enzyme, the inhibition of which allows initiation of the signalling pathway. This study aims to utilise a binary SiO2-Li2O sol-gel derived glass for controlled delivery of lithium, a known glycogen synthase kinase-3 antagonist...
July 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28618870/molecular-validation-of-chondrogenic-differentiation-and-hypoxia-responsiveness-of-platelet-lysate-expanded-adipose-tissue-derived-human-mesenchymal-stromal-cells
#9
Catalina Galeano-Garces, Emily T Camilleri, Scott M Riester, Amel Dudakovic, Dirk R Larson, Wenchun Qu, Jay Smith, Allan B Dietz, Hee-Jeong Im, Aaron J Krych, A Noelle Larson, Marcel Karperien, Andre J van Wijnen
OBJECTIVE: To determine the optimal environmental conditions for chondrogenic differentiation of human adipose tissue-derived mesenchymal stromal/stem cells (AMSCs). In this investigation we specifically investigate the role of oxygen tension and 3-dimensional (3D) culture systems. DESIGN: Both AMSCs and primary human chondrocytes were cultured for 21 days in chondrogenic media under normoxic (21% oxygen) or hypoxic (2% oxygen) conditions using 2 distinct 3D culture methods (high-density pellets and poly-ε-caprolactone [PCL] scaffolds)...
July 2017: Cartilage
https://www.readbyqxmd.com/read/28618041/effect-of-various-ratios-of-co-cultured-atdc5-cells-and-chondrocytes-on-the-expression-of-cartilaginous-phenotype-in-microcavitary-alginate-hydrogel
#10
Yongchang Yao, Yuyang Huang, Dongyang Qian, Shujiang Zhang, Yi Chen, Bo Bai
The present study introduced a direct co-culture of mouse ATDC5 cells and primary porcine chondrocytes into a microcavitary hydrogel, which possessed advantages in promoting the growth of chondrocytes and retaining the phenotype. These two types of cells were encapsulated with gelatin microspheres in alginate hydrogels in either of the three ratios (3:1, 1:1, or 1:3 of ATDC5 cells to chondrocytes) and cultured in chondrogenic medium for 28 days. Simultaneously, the single encapsulation of ATDC5 cells or chondrocytes was set as a control...
June 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28616967/effects-of-functional-groups-of-materials-on-nonspecific-adhesion-and-chondrogenic-induction-of-mesenchymal-stem-cells-on-free-and-micropatterned-surfaces
#11
Bin Cao, Yuanmeng Peng, Xiangnan Liu, Jiandong Ding
Functional groups of materials are known to affect cell behaviors, yet the corresponding effect on stem cell differentiation are always coupled with that of cell spreading, it is thus unclear whether or not the chemical groups influence cell differentiation directly or via cell spreading indirectly. Herein we used a unique surface patterning technique to decouple the corresponding effects. Mesenchymal stem cells (MSCs) derived from bone marrow were seeded on surfaces coated with alkylthiols with one of 4 functional end groups (-CH3, -OH, -COOH and -NH2) and underwent 9-day chondrogenic induction...
June 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28616703/preparation-of-a-biphase-composite-scaffold-and-its-application-in-tissue-engineering-for-femoral-osteochondral-defects-in-rabbits
#12
Shi-Qiang Ruan, Ling Yan, Jiang Deng, Wen-Liang Huang, Dian-Ming Jiang
PURPOSE: Three-dimensional bioactive scaffolds are useful tools for stem cell implant in tissue-engineering. For chondral and subchondral repair, the chondroinductive and osteoinductive property of a scaffold is a major challenge. The scaffolds that aim to osteogenic differentiation have been well studied. However, cartilage cells can hardly be induced for osteogenesis, and monophase scaffolds cannot ideally repair both cartilage and subchondral defects at the same time. METHODS: We developed a novel biphase composite scaffold and observe its application osteochondral defects...
June 14, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28611369/enhanced-chondrogenesis-of-bone-marrow-derived-stem-cells-by-using-a-combinatory-cell-therapy-strategy-with-bmp-2-tgf-%C3%AE-1-hypoxia-and-col1a1-htra1-sirnas
#13
Florence Legendre, David Ollitrault, Tangni Gomez-Leduc, Mouloud Bouyoucef, Magalie Hervieu, Nicolas Gruchy, Frédéric Mallein-Gerin, Sylvain Leclercq, Magali Demoor, Philippe Galéra
Mesenchymal stem cells (MSCs) hold promise for cartilage engineering. Here, we aimed to determine the best culture conditions to induce chondrogenesis of MSCs isolated from bone marrow (BM) of aged osteoarthritis (OA) patients. We showed that these BM-MSCs proliferate slowly, are not uniformly positive for stem cell markers, and maintain their multilineage potential throughout multiple passages. The chondrogenic lineage of BM-MSCs was induced in collagen scaffolds, under normoxia or hypoxia, by BMP-2 and/or TGF-β1...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28611002/chondrogenesis-of-human-bone-marrow-mesenchymal-stem-cells-in-3-dimensional-photocrosslinked-hydrogel-constructs-effect-of-cell-seeding-density-and-material-stiffness
#14
Aaron X Sun, Hang Lin, Madalyn R Fritch, He Shen, Pete G Alexander, Michael DeHart, Rocky S Tuan
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage. In particular, hydrogel scaffolds that augment chondrogenesis and recapitulate the native physical properties of cartilage, such as compressive strength, can potentially be applied in point-of-care procedures. We report here the synthesis of two new materials, [poly-l-lactic acid/polyethylene glycol/poly-l-lactic acid] (PLLA-PEG 1000) and [poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid] (PDLLA-PEG 1000), that are biodegradable, biocompatible (>80% viability post fabrication), and possess high, physiologically relevant mechanical strength (∼1500 to 1800kPa)...
June 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28610596/complete-human-serum-maintains-viability-and-chondrogenic-potential-of-human-synovial-stem-cells-suitable-conditions-for-transplantation
#15
Mitsuru Mizuno, Hisako Katano, Koji Otabe, Keiichiro Komori, Yuji Kohno, Shizuka Fujii, Nobutake Ozeki, Masafumi Horie, Kunikazu Tsuji, Hideyuki Koga, Takeshi Muneta, Ichiro Sekiya
BACKGROUND: In our clinical practice, we perform transplantations of autologous synovial mesenchymal stem cells (MSCs) for cartilage and meniscus regenerative medicine. One of the most important issues to ensuring clinical efficacy involves the transport of synovial MSCs from the processing facility to the clinic. Complete human serum (100% human serum) is an attractive candidate material in which to suspend synovial MSCs for their preservation during transport. The purpose of this study was to investigate whether complete human serum maintained MSC viability and chondrogenic potential and to examine the optimal temperature conditions for the preservation of human synovial MSCs...
June 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28610480/induced-re-differentiation-of-human-chondrocytes-from-articular-cartilage-lesion-in-alginate-bead-culture-after-monolayer-de-differentiation-an-alternative-cell-source-for-cell-based-therapies
#16
Matthias Aurich, Gunther O Hofmann, Norman Best, Bernd Rolauffs
Human chondrocytes isolated from articular cartilage lesions as an alternative cell source to the standard non-weight-bearing notch biopsy site may hold clinical potential for cell-based therapies. The aim was to characterize human AC lesion site chondrocytes, compare them to notch chondrocytes, and to evaluate their re-differentiation potential after monolayer expansion and subsequent 3D alginate bead culture. Lesion chondrocytes from knee joints of 20 patients with ICRS grade 3 and 4 cartilage defects were analyzed ex vivo or cultured in primary alginate bead culture, monolayer expansion, or were re-differentiated in alginate culture following monolayer expansion...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28609018/pcl-phbv-blended-three-dimensional-scaffolds-fabricated-by-fused-deposition-modeling-and-responses-of-chondrocytes-to-the-scaffolds
#17
Wasana Kosorn, Morakot Sakulsumbat, Paweena Uppanan, Pakkanun Kaewkong, Surapol Chantaweroad, Jaturong Jitsaard, Kriskrai Sitthiseripratip, Wanida Janvikul
In this study, poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV) blended porous scaffolds were fabricated by fused deposition modeling (FDM). PCL/PHBV filaments, initially prepared at different weight ratios, that is, 100/0, 75/25, 50/50, and 25/75, were fabricated by the lay-down pattern of 0/90/45/135° to obtain scaffolds with dimension of 6.0 × 6.0 × 2.5 mm(3) and average filament diameters and channel sizes in the ranges of 370-390 µm and 190-210 µm, respectively. To enhance the surface hydrophilicity of the materials, the scaffolds were subsequently subjected to a low pressure oxygen plasma treatment...
July 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28607560/characterization-of-mesenchymal-stem-cell-like-cells-derived-from-human-ipscs-via-neural-crest-development-and-their-application-for-osteochondral-repair
#18
Ryota Chijimatsu, Makoto Ikeya, Yukihiko Yasui, Yasutoshi Ikeda, Kosuke Ebina, Yu Moriguchi, Kazunori Shimomura, David A Hart, Yoshikawa Hideki, Nakamura Norimasa
Mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) are a promising cell source for the repair of skeletal disorders. Recently, neural crest cells (NCCs) were reported to be effective for inducing mesenchymal progenitors, which have potential to differentiate into osteochondral lineages. Our aim was to investigate the feasibility of MSC-like cells originated from iPSCs via NCCs for osteochondral repair. Initially, MSC-like cells derived from iPSC-NCCs (iNCCs) were generated and characterized in vitro...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28607469/pth-1-34-induced-differentiation-and-mineralization-of-mandibular-condylar-cartilage
#19
Mara Heather O' Brien, Eliane Hermes Dutra, Alexandro Lima, Ravindra Nanda, Sumit Yadav
Intermittent Parathyroid Hormone (I-PTH) is the only FDA approved anabolic drug therapy available for the treatment of osteoporosis in males and postmenopausal females. The effects of I-PTH on the chondrogenic lineage of the mandibular condylar cartilage (MCC) are not well understood. To investigate the role of I-PTH on the MCC and subchondral bone, we carried out our studies using 4 to 5 week old triple transgenic mice (Col1a1XCol2a1XCol10a1). The experimental group was injected with PTH (80 μg/kg) daily for 2 weeks, while control group was injected with saline...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28606838/the-role-of-low-intensity-pulse-ultrasound-lipus-on-cartilage-healing-in-knee-osteoarthritis-a-review
#20
REVIEW
Joshua Rothenberg, Prathap Jayaram, Usker Naqvi, Joslyn Gober, Gerard A Malanga
Ultrasound (US) is a therapeutic modality that has been used in the treatment of musculoskeletal conditions for decades. In recent years there has been technological advancements using Low-Intensity Pulsed Ultrasound (LIPUS) as a clinical modality. The purpose of this review was to critically examine the medical literature to determine the effects of low-intensity pulse ultrasound (LIPUS) on the chondrogenic properties of knee osteoarthritis. A literature search of three major databases (Pubmed, Scopus, EMBASE) was performed...
June 9, 2017: PM & R: the Journal of Injury, Function, and Rehabilitation
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