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https://www.readbyqxmd.com/read/28441755/role-of-rheb-in-regulating-differentiation-fate-of-mesenchymal-stem-cells-for-cartilage-and-bone-regeneration
#1
Sajjad Ashraf, In-Bo Han, Hansoo Park, Soo-Hong Lee
Advances in mesenchymal stem cells (MSCs) and cell replacement therapies are promising approaches to treat cartilage and bone defects since substantial differentiation capacities of MSCs match the demands of tissue regeneration. Our understanding of the dynamic process requiring indispensable differentiation of MSCs remains limited. Herein, we describe the role of RHEB (Ras homolog enriched in brain) regulating gene signature for differentiation of human adipose derived mesenchymal stem cells (ASCs) into chondrogenic, osteogenic, and adipogenic lineages...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28438716/yap1-negatively-regulates-chondrocyte-differentiation-partly-by-activating-the-%C3%AE-catenin-signaling-pathway
#2
Beining Yang, Hualing Sun, Fangfang Song, Miao Yu, Yanru Wu, Jiawei Wang
YAP1 (Yes-associated protein 1) transcriptional coactivator is a downstream gene of the Hippo signaling pathway, which controls cell proliferation and differentiation. YAP1 plays a significant role in the regulation of cartilage and bone development. However, the molecular mechanism by which YAP1 regulates chondrocyte differentiation remains to be elucidated. Immunofluorescent staining was used to visualize the localization of YAP1 expression in the mouse chondroprogenitor ATDC5 cell line. ATDC5 cells with lentivirus-vector-mediated YAP1 overexpression and knockdown were established...
April 21, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28436462/mir-146b-is-down-regulated-during-the-chondrogenic-differentiation-of-human-bone-marrow-derived-skeletal-stem-cells-and-up-regulated-in-osteoarthritis
#3
Emma Budd, María C de Andrés, Tilman Sanchez-Elsner, Richard O C Oreffo
Articular cartilage injury can result in chondrocyte loss and diminishment of specialised extracellular matrix, which can progress to an osteoarthritic (OA) phenotype. Stem cells have emerged as a favourable approach for articular cartilage regeneration. Identification of miRNAs which influence stem cell fate offers new approaches for application of miRNAs to regenerate articular cartilage. Skeletal stem cells (SSCs) isolated from human bone marrow were cultured as high density micromass' using TGF-β3 to induce chondrogenesis...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28435520/genetically-transforming-human-osteoblasts-to-sarcoma-development-of-an-osteosarcoma-model
#4
Yi Yang, Rui Yang, Michael Roth, Sajida Piperdi, Wendong Zhang, Howard Dorfman, Pulivarthi Rao, Amy Park, Sandeep Tripathi, Carrie Freeman, Yunjia Zhang, Rebecca Sowers, Jeremy Rosenblum, David Geller, Bang Hoang, Jonathan Gill, Richard Gorlick
Osteosarcoma is the most common primary malignant bone tumor in children and young adults. Although histologically defined by the presence of malignant osteoid, the tumor possesses lineage multipotency suggesting it could be derived from a cell anywhere on the differentiation pathway between a mesenchymal stem cell (MSC) and a mature osteoblast. To determine if preosteoblasts (pOB) could be the cell of origin differentiated MSCs were transformed with defined genetic elements. MSCs and pOB differentiated from the same MSCs were serially transformed with the oncogenes hTERT, SV40 large T antigen and H-Ras...
January 2017: Genes & Cancer
https://www.readbyqxmd.com/read/28434038/emerging-potential-of-gene-silencing-approaches-targeting-anti-chondrogenic-factors-for-cell-based-cartilage-repair
#5
REVIEW
Andrea Lolli, Letizia Penolazzi, Roberto Narcisi, Gerjo J V M van Osch, Roberta Piva
The field of cartilage repair has exponentially been growing over the past decade. Here, we discuss the possibility to achieve satisfactory regeneration of articular cartilage by means of human mesenchymal stem cells (hMSCs) depleted of anti-chondrogenic factors and implanted in the site of injury. Different types of molecules including transcription factors, transcriptional co-regulators, secreted proteins, and microRNAs have recently been identified as negative modulators of chondroprogenitor differentiation and chondrocyte function...
April 22, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28432956/introduction-of-n-cadherin-binding-motif-to-alginate-hydrogels-for-controlled-stem-cell-differentiation
#6
Jae Won Lee, Hyoseok An, Kuen Yong Lee
Control of stem cell fate and phenotype using biomimetic synthetic extracellular matrices (ECMs) is an important tissue engineering approach. Many studies have focused on improving cell-matrix interactions. However, proper control of cell-cell interactions using synthetic ECMs could be critical for tissue engineering, especially with undifferentiated stem cells. In this study, alginate hydrogels were modified with a peptide derived from the low-density lipoprotein receptor-related protein 5 (LRP5), which is known to bind to N-cadherin, as a cell-cell interaction motif...
April 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28425567/effect-of-ngf-from-venom-of-chinese-cobra-naja-atra-on-chondrocytes-proliferation-and-metabolism-in-vitro
#7
Jiachang Tan, Zhenhui Lu, Zhikang Miao, Danqing Lei, Li Zheng, Jinmin Zhao
Autologous chondrocyte implantation (ACI) is promising strategy for cartilage repair. However, chondrocyte phenotype is easily lost when expanded in vitro which defined as "dedifferentiation". To ensure successful therapy, an effective pro-chondrogenic agent is necessary to overcome the obstacle of limited cell numbers in the restoration process, and dedifferentiation is a prerequisite. In the present study, we investigated the chondro-protective effect of NGF from Chinese cobra venom on human chondrocytes by determination of its specific effect on cell viability, proliferation, morphology, GAG production and cartilage specific gene expression...
April 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28420812/isolation-of-mesenchymal-stromal-stem-cells-from-cryopreserved-umbilical-cord-blood-cells
#8
Sumie Fujii, Yasuo Miura, Masaki Iwasa, Satoshi Yoshioka, Aya Fujishiro, Noriko Sugino, Hitomi Kaneko, Yoko Nakagawa, Hideyo Hirai, Akifumi Takaori-Kondo, Tatsuo Ichinohe, Taira Maekawa
Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×10(8)) that were processed and cryopreserved using CP-1/human serum albumin. This isolation rate was lower than that (57%) from non-cryopreserved UCB cells...
April 18, 2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28420447/ck2-1-a-bone-morphogenetic-protein-receptor-type-ia-mimetic-peptide-repairs-cartilage-in-mice-with-destabilized-medial-meniscus
#9
Hemanth Akkiraju, Padma Pradeepa Srinivasan, Xian Xu, Xinqiao Jia, Catherine B Kirn Safran, Anja Nohe
BACKGROUND: Osteoarthritis (OA) of the knee involves degeneration of articular cartilage of the diarthrodial joints. Current treatment options temporarily relieve the joint pain but do not restore the lost cartilage. We recently designed a novel bone morphogenetic protein receptor type I (BMPRI) mimetic peptide, CK2.1, that activates BMPRIa signaling in the absence of bone morphogenetic protein (BMP). Our previous research demonstrated that CK2.1 induced chondrogenesis in vitro and in vivo; however, it is unknown if CK2...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28419571/characterization-of-mesenchymal-stem-cells-in-bovine-endometrium-during-follicular-phase-of-oestrous-cycle
#10
E Lara, N Rivera, D Rojas, L L Rodríguez-Alvarez, F O Castro
Stem cells have been postulated as responsible for cell regeneration in highly and continuously regenerative tissues such as the endometrium. Few studies in cattle have identified and specified the presence of stem cells in the endometrium during the oestrous cycle. The aim of this study was to investigate the presence of mesenchymal stem cells (MSCs) in the bovine endometrium during the follicular phase (FP) of the oestrous cycle. Uterine tissue was collected in the time-frame comprising day 18 of the cycle and ovulation (day 0)...
April 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28414616/biphasic-finite-element-modeling-reconciles-mechanical-properties-of-tissue-engineered-cartilage-constructs-across-testing-platforms
#11
Gregory R Meloni, Matthew B Fisher, Brendan D Stoeckl, George R Dodge, Robert L Mauck
Cartilage tissue engineering is emerging as a promising treatment for osteoarthritis, and the field has progressed toward utilizing large animal models for proof of concept and preclinical studies. Mechanical testing of the regenerative tissue is an essential outcome for functional evaluation. However, testing modalities and constitutive frameworks used to evaluate in vitro grown samples differ substantially from those used to evaluate in vivo derived samples. To address this, we developed finite element (FE) models (using FEBio) of unconfined compression and indentation testing, modalities commonly used for such samples...
April 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28410428/tuft1-a-novel-candidate-gene-for-metatarsophalangeal-osteoarthritis-plays-a-role-in-chondrogenesis-on-a-calcium-related-pathway
#12
Eeva Sliz, Mari Taipale, Maiju Welling, Sini Skarp, Viivi Alaraudanjoki, Jaakko Ignatius, Lloyd Ruddock, Ritva Nissi, Minna Männikkö
Osteoarthritis (OA) is the most common degenerative joint disorder and genetic factors have been shown to have a significant role in its etiology. The first metatarsophalangeal joint (MTP I) is highly susceptible to development of OA due to repetitive mechanical stress during walking. We used whole exome sequencing to study genetic defect(s) predisposing to familial early-onset bilateral MTP I OA inherited in an autosomal dominant manner. A nonsynonymous single nucleotide variant rs41310883 (c.524C>T, p...
2017: PloS One
https://www.readbyqxmd.com/read/28409292/effects-of-the-donor-age-on-proliferation-senescence-and-osteogenic-capacity-of-human-urine-derived-stem-cells
#13
Peng Gao, Peilin Han, Dapeng Jiang, Shulong Yang, Qingbo Cui, Zhaozhu Li
To study the effects of the donor age on the application potential of human urine-derived stem cells (hUSCs) in bone tissue engineering, by comparing proliferation, senescence and osteogenic differentiation of hUSCs originated from volunteers with different ages. The urine samples were collected from 19 healthy volunteers (6 cases from children group aged from 5 to 14, 5 cases from middle-aged group aged from 30 to 40, and 8 cases from the elder group aged from 65 to 75), and hUSCs were isolated and cultured...
April 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28405836/chondrogenesis-and-hypertrophy-in-response-to-aggregate-behaviors-of-human-mesenchymal-stem-cells-on-a-dendrimer-immobilized-surface
#14
Sopita Wongin, Yuuki Ogawa, Mee-Hae Kim, Kwanchanok Viravaidya-Pasuwat, Masahiro Kino-Oka
OBJECTIVES: To investigate the behaviors of aggregates of human mesenchymal stem cells (hMSCs) on chondrogenesis and chondrocyte hypertrophy using spatiotemporal expression patterns of chondrogenic (type II collagen) and hypertrophic (type X collagen) markers during chondrogenesis. RESULTS: hMSCs were cultured on either a polystyrene surface or polyamidoamine dendrimer surface with a fifth generation (G5) dendron structure in chondrogenic medium and growth medium...
April 12, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28396584/a-high-glucose-diet-affects-achilles-tendon-healing-in-rats
#15
Stefanie Korntner, Nadja Kunkel, Christine Lehner, Renate Gehwolf, Andrea Wagner, Peter Augat, Daniel Stephan, Verena Heu, Hans-Christian Bauer, Andreas Traweger, Herbert Tempfer
Chronic and acute tendinopathies are difficult to treat and tendon healing is generally a very slow and incomplete process and our general understanding of tendon biology and regeneration lags behind that of muscle or bone. Although still largely unexplored, several studies suggest a positive effect of nutritional interventions on tendon health and repair. With this study, we aim to reveal effects of a high-glucose diet on tendon neoformation in a non-diabetic rat model of Achilles tenotomy. After surgery animals received either a high-glucose diet or a control diet for 2 and 4 weeks, respectively...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28389839/p15-peptide-stimulates-chondrogenic-commitment-and-endochondral-ossification
#16
Jun Zhang, Peter Eisenhauer, Ozҫan Kaya, Alexander R Vaccaro, Carol Diallo, Andrzej Fertala, Theresa A Freeman
PURPOSE: The synthetic 15 amino acid biomimetic peptide sequence (P15) derived from a region of the alpha (α)-1 chain of collagen I, has been shown to promote α2 integrin activation and enhance intramembranous ossification. In this study, we ask if the P15 peptide also enhances bone formation through endochondral ossification, and determine if direct binding of α2 integrin with P15 mediates integrin activation. METHODS: Mesenchymal cells (C3H10T1/2) were cultured in chondrogenic media and the expression of chondrogenic markers and integrin activation was determined by Western blot and fluorescent immunohistochemistry...
April 8, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28385113/hypoxia-for-msc-expansion-and-differentiation-the-best-way-for-enhancing-tgf%C3%A3-induced-chondrogenesis-and-preventing-calcifications-in-alginate-beads
#17
Christel Henrionnet, Gai Liang, Emilie Roeder, Manuel Dossot, Hui Wang, Jacques Magdalou, Pierre Gillet, Astrid Pinzano
We examined the respective influence of a sequential or a continuous hypoxia during expansion and TGF-ß1-driven chondrogenic differentiation of human bone marrow mesenchymal stem cells (MSCs). The differentiation was performed within alginate beads, a classical tool for the implantation of MSCs within the joint. The standard normoxic 2D (expansion) and 3D (differentiation) MSCs cultures served as reference. In order to determine the quality of the chondrogenesis, we analyzed typical markers such as type II and X collagens, SOX9, COMP, versican, and aggrecan mRNAs using PCR and we assessed the production of type II collagen and HIF-1α by histological stainings...
April 7, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28375818/chondrocyte-attachment-proliferation-and-differentiation-on-three-dimensional-polycaprolactone-fumarate-scaffolds
#18
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J van Wijnen, Michael J Yaszemski, Sanjeev Kakar
Current treatment options for cartilage injuries are limited. The goals of this study are to create a biodegradable polymer scaffold with the capabilities of sustaining chondrocyte growth and proliferation, enable cell-to-cell communication and tissue regeneration through large pores, and assess the biological augmentation of the scaffold capabilities using platelet lysate (PL). We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow cell-cell communication through large interconnected pores...
March 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28374574/a-comparative-assessment-of-adipose-derived-stem-cells-from-subcutaneous-and-visceral-fat-as-a-potential-cell-source-for-knee-osteoarthritis-treatment
#19
Yan Tang, Zhang-Yi Pan, Ying Zou, Yi He, Peng-Yuan Yang, Qi-Qun Tang, Feng Yin
The intra-articular injection of adipose-derived stem cells (ASCs) is a novel potential therapy for patients with osteoarthritis (OA). However, the efficacy of ASCs from different regions of the body remains unknown. This study investigated whether ASCs from subcutaneous or visceral adipose tissue provide the same improvement of OA. Mouse and human subcutaneous and visceral adipose tissue were excised for ASC isolation. Morphology, proliferation, surface markers and adipocyte differentiation of subcutaneous ASCs (S-ASCs) and visceral ASCs (V-ASCs) were analysed...
April 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28372298/effect-of-nicotine-on-the-proliferation-and-chondrogenic-differentiation-of-the-human-wharton-s-jelly-mesenchymal-stem-cells
#20
Xu Yang, Yongjian Qi, Léonore Avercenc-Leger, Jean-Baptiste Vincourt, Sébastien Hupont, Céline Huselstein, Hui Wang, Liaobin Chen, Jacques Magdalou
BACKGROUND: Osteoarthritis (OA) is a chronic joint disease characterized by a progressive and irreversible degeneration of articular cartilage. Among the environmental risk factors of OA, tobacco consumption features prominently, although, there is a great controversy regarding the role of tobacco smoking in OA development. Among the numerous chemicals present in cigarette smoke, nicotine is one of the most physiologically active molecules. OBJECTIVE: The aim of the study was (i) to measure the impact of nicotine on the proliferation and chondrogenic differentiation of mesenchymal stem cells from the human Wharton's jelly (hWJ-MSCs) into chondrocytes, (ii) to investigate whether the α7 nicotinic acetylcholine receptors (nAChRs) was expressed in hWJ-MSCs and could play a role in the process...
2017: Bio-medical Materials and Engineering
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