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https://www.readbyqxmd.com/read/27911782/egfr-signaling-is-critical-for-maintaining-the-superficial-layer-of-articular-cartilage-and-preventing-osteoarthritis-initiation
#1
Haoruo Jia, Xiaoyuan Ma, Wei Tong, Basak Doyran, Zeyang Sun, Luqiang Wang, Xianrong Zhang, Yilu Zhou, Farid Badar, Abhishek Chandra, X Lucas Lu, Yang Xia, Lin Han, Motomi Enomoto-Iwamoto, Ling Qin
Osteoarthritis (OA) is the most common joint disease, characterized by progressive destruction of the articular cartilage. The surface of joint cartilage is the first defensive and affected site of OA, but our knowledge of genesis and homeostasis of this superficial zone is scarce. EGFR signaling is important for tissue homeostasis. Immunostaining revealed that its activity is mostly dominant in the superficial layer of healthy cartilage but greatly diminished when OA initiates. To evaluate the role of EGFR signaling in the articular cartilage, we studied a cartilage-specific Egfr-deficient (CKO) mouse model (Col2-Cre EgfrWa5/flox)...
November 28, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27907211/characterization-and-immunomodulatory-effects-of-canine-adipose-tissue-and-bone-marrow-derived-mesenchymal-stromal-cells
#2
Keith A Russell, Natalie H C Chow, David Dukoff, Thomas W G Gibson, Jonathan LaMarre, Dean H Betts, Thomas G Koch
BACKGROUND: Mesenchymal stromal cells (MSC) hold promise for both cell replacement and immune modulation strategies owing to their progenitor and non-progenitor functions, respectively. Characterization of MSC from different sources is an important and necessary step before clinical use of these cells is widely adopted. Little is known about the biology and function of canine MSC compared to their mouse or human counterparts. This knowledge-gap impedes development of canine evidence-based MSC technologies...
2016: PloS One
https://www.readbyqxmd.com/read/27903449/sex-differences-in-the-estrogen-dependent-regulation-of-temporomandibular-joint-remodeling-in-altered-loading
#3
Jennifer L Robinson, Katelyn Cass, Ross Aronson, Thomas Choi, Manshan Xu, Ryan Buttenbaum, Hicham Drissi, Helen H Lu, Jing Chen, Sunil Wadhwa
OBJECTIVE: Temporomandibular joint (TMJ) diseases predominantly afflict women, suggesting a role of estrogen in the disease etiology. Previously, we determined that decreased occlusal loading (DOL) inhibited collagen type II (Col2) expression in the mandibular condylar cartilage (MCC) of female wild-type (WT) mice whereas no change was observed in males. This decrease in chondrogenesis was abolished by estrogen receptor beta (ERβ) deficiency in females. Therefore, the goal of this study was to examine the role of estradiol - ERβ signaling in mediating DOL effects in male mice to further decipher sex differences...
November 26, 2016: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/27900891/chondrogenesis-of-human-adipose-derived-stem-cells-for-future-microtia-repair-using-co-culture-technique
#4
Bee See Goh, Siti Nurhadis Che Omar, Muhammad Azhan Ubaidah, Lokman Saim, Shamsul Sulaiman, Kien Hui Chua
CONCLUSION: In conclusion, these result showed HADSCs could differentiate into chondrocytes-like cells, dependent on signaling induced by TGF-β3 and chondrocytes. This is a promising result and showed that HADSCs is a potential source for future microtia repair. The technique of co-culture is a positive way forward to assist the microtia tissue. OBJECTIVE: Reconstructive surgery for the repair of microtia still remains the greatest challenge among the surgeons...
November 30, 2016: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27900293/combined-use-of-platelet-rich-plasma-micro-fat-in-sport-and-race-horses-with-degenerative-joint-disease-preliminary-clinical-study-in-eight-horses
#5
Fabrizio Bembo, Julia Eraud, Cecile Philandrianos, Baptiste Bertrand, Alain Silvestre, Julie Veran, Florence Sabatier, Guy Magalon, Jeremy Magalon
BACKGROUND: To assess the safety and potential efficacy of a standardized technique consisting of intra-articular injection of 10 cc of a homogeneous mixed product using autologous micro-fat and platelet rich plasma (PRP) (ratio 1:1) in the carpus or the fetlock joint of sport horses presenting degenerative joint disease (DJD). METHODS: Eight sport horses with DJD confirmed by radiography and ultrasonography and causing lameness and the impossibility to compete were treated...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27899317/neurotrophic-factor-mediated-neuronal-differentiation-of-human-cord-blood-mesenchymal-stem-cells-and-their-applicability-to-assess-the-developmental-neurotoxicity
#6
Sadaf Jahan, Dipak Kumar, Ashvini Kumar, Chetan Singh Rajpurohit, Shripriya Singh, Akriti Srivastava, Ankita Pandey, A B Pant
Plasticity and developmental capacity of stem cells have now been established as a promising tool to restore the degenerative disorders. The linearity differentiation of human mesenchymal stem cells (hMSCs) into adipogenic, chondrogenic, osteogenic and even in neuronal subtypes has been demonstrated. The number of xenobiotics such as dexamethasone, insulin, isobutyl 1-methyle xanthine and retinoic acid has been reported for the potential to differentiate hMSCs into neuronal subtypes. But, the applicability of indigenous neurotrophic factor-nerve growth factor (NGF) has not been explored for the purpose...
November 27, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27899238/goat-umbilical-cord-cells-are-permissive-to-small-ruminant-lentivirus-infection-in-vitro
#7
Gabrielle R Martins, Rebeca C Marinho, Rosivaldo Q Bezerra Junior, Antoniel de O Alves, Lilia M C Câmara, Luiz C Albuquerque-Pinto, Maria F da S Teixeira
Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture...
November 19, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27898233/hyaluronan-stimulates-chondrogenic-gene-expression-in-human-meniscus-cells
#8
Takaaki Tanaka, Takayuki Furumatsu, Shinichi Miyazawa, Masataka Fujii, Hiroto Inoue, Yuya Kodama, Toshifumi Ozaki
: Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. MATERIALS AND METHODS: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco's modified Eagle's medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2-7 days...
November 29, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#9
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27894394/differences-of-cell-surface-marker-expression-between-bone-marrow-and-kidney-derived-murine-mesenchymal-stromal-cells-and-fibroblasts
#10
F Cakiroglu, J W Osbahr, J Kramer, J Rohwedel
Mesenchymal stromal cells (MSC) are undifferentiated, multipotent adult cells with regenerative properties. They are particularly relevant for therapeutic approaches due to the simplicity of their isolation and cultivation. Since MSC show an expression pattern of cell surface marker, which is almost identical to fibroblasts, many attempts have been made to address the similarities and differences between MSC and fibroblasts. In this study we aimed to isolate murine MSC from bone marrow (BM) and kidney to characterize them in comparison to fibroblasts...
October 31, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27892651/fumarate-copolymer-chitosan-cross-linked-scaffold-directed-to-osteochondrogenic-tissue-engineering
#11
María Laura Lastra, María Silvina Molinuevo, Ana María Cortizo, María Susana Cortizo
Natural and synthetic cross-linked polymers allow the improvement of cytocompatibility and mechanical properties of the individual polymers. In osteochondral lesions of big size it will be required the use of scaffolds to repair the lesion. In this work a borax cross-linked scaffold based on fumarate-vinyl acetate copolymer and chitosan directed to osteochondrondral tissue engineering is developed. The cross-linked scaffolds and physical blends of the polymers are analyzed in based on their morphology, glass transition temperature, and mechanical properties...
November 28, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27890611/histone-methyltransferase-setdb1-is-indispensable-for-meckel-s-cartilage-development
#12
Kohei Yahiro, Norihisa Higashihori, Keiji Moriyama
The histone methyltransferase Setdb1 represses gene expression by catalyzing lysine 9 of histone H3 trimethylation. Given that the conventional knockout of Setdb1 is embryo-lethal at the implantation stage, its role in craniofacial development is poorly understood. Here, we investigated the role of Setdb1, using conditional knockout mice-in which Setdb1 was deleted in the Meckel's cartilage (Setdb1 CKO)-and the mouse chondrogenic cell line ATDC5-in which Setdb1 was inhibited by siRNA. Deletion of Setdb1 in Meckel's cartilage, the supportive tissue in the embryonic mandible, led to its enlargement, instead of the degeneration that normally occurs...
November 24, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27890430/tar-dna-binding-protein-43-inhibits-inflammatory-response-and-protects-chondrocyte-function-by-modulating-rack1-expression-in-osteoarthritis
#13
Yongming Huang, Qiming Huang, Haitao Su, Xiujun Mai, Enhui Feng, Zhenwu Cao, Xiuyun Zeng
BACKGROUND: TAR DNA-binding protein-43 (TDP-43, transactive response DNA binding protein 43kDa) accumulates in the cytoplasm of affected neurons and glia, as large inclusions of stress granules (SGs). However, the mechanism of TDP-43 interaction with the target genes and its specific role in osteoarthritis (OA) progression is still unknown. The goal of this study was to identify the role of TDP-43 in OA progression by modulating its target genes. METHODS: MSCs were transfected with TDP-43 gene lentivirus...
November 24, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27887732/expressions-of-pathologic-markers-in-prp-based-chondrogenic-differentiation-of-human-adipose-derived-stem-cells
#14
Arezou Pakfar, Shiva Irani, Hana Hanaee-Ahvaz
BACKGROUND: Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP...
November 12, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27886065/dual-function-of-glucosamine-in-gelatin-hyaluronic-acid-cryogel-to-modulate-scaffold-mechanical-properties-and-to-maintain-chondrogenic-phenotype-for-cartilage-tissue-engineering
#15
Chih-Hao Chen, Chang-Yi Kuo, Yan-Jie Wang, Jyh-Ping Chen
Glucosamine (GlcN) fulfills many of the requirements as an ideal component in scaffolds used in cartilage tissue engineering. The incorporation of GlcN in a gelatin/hyaluronic acid (GH) cryogel scaffold could provide biological cues in maintaining the phenotype of chondrocytes. Nonetheless, substituting gelatin with GlcN may also decrease the crosslinking density and modulate the mechanical properties of the cryogel scaffold, which may be beneficial as physical cues for chondrocytes in the scaffold. Thus, we prepared cryogel scaffolds containing 9% GlcN (GH-GlcN9) and 16% GlcN (GH-GlcN16) by carbodiimide-mediated crosslinking reactions at -16 °C...
November 23, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27885573/poly-%C3%AE-glutamic-acid-and-poly-%C3%AE-glutamic-acid-based-nanocomplexes-enhance-type-ii-collagen-production-in-intervertebral-disc
#16
Joana C Antunes, Catarina Leite Pereira, Graciosa Q Teixeira, Ricardo V Silva, Joana Caldeira, Sibylle Grad, Raquel M Gonçalves, Mário A Barbosa
Intervertebral disc (IVD) degeneration often leads to low back pain, which is one of the major causes of disability worldwide, affecting more than 80% of the population. Although available treatments for degenerated IVD decrease symptoms' progression, they fail to address the underlying causes and to restore native IVD properties. Poly(γ-glutamic acid) (γ-PGA) has recently been shown to support the production of chondrogenic matrix by mesenchymal stem/stromal cells. γ-PGA/chitosan (Ch) nanocomplexes (NCs) have been proposed for several biomedical applications, showing advantages compared with either polymer alone...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27884646/microrna-92a-3p-regulates-the-expression-of-cartilage-specific-genes-by-directly-targeting-histone-deacetylase-2-in-chondrogenesis-and-degradation
#17
Guping Mao, Ziji Zhang, Zhiyu Huang, Weishen Chen, Guangxin Huang, Fangang Meng, Zhiqi Zhang, Yan Kang
OBJECTIVE: Increased activity of histone deacetylase 2 (HDAC2) has been found in patients with osteoarthritis (OA) and cartilage matrix degradation and has been shown to mediate the repression of cartilage-specific gene expression in human chondrocytes. We aimed to determine whether microRNA-92a-3p (miR-92a-3p) regulates cartilage-specific gene expression via targeted HDAC2 in chondrogenesis and degradation. METHODS: miR-92a-3p expression was assessed in vitro in a human mesenchymal stem cells (hMSCs) model of chondrogenesis and in normal and OA primary human chondrocytes (PHCs), and in normal and OA human cartilage by in situ hybridization...
November 21, 2016: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/27883051/lipid-coated-microbubbles-and-low-intensity-pulsed-ultrasound-enhance-chondrogenesis-of-human-mesenchymal-stem-cells-in-3d-printed-scaffolds
#18
Mitra Aliabouzar, Lijie Grace Zhang, Kausik Sarkar
Lipid-coated microbubbles are used to enhance ultrasound imaging and drug delivery. Here we apply these microbubbles along with low intensity pulsed ultrasound (LIPUS) for the first time to enhance proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in a 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) hydrogel scaffold. The hMSC proliferation increased up to 40% after 5 days of culture in the presence of 0.5% (v/v) microbubbles and LIPUS in contrast to 18% with LIPUS alone...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881824/two-tissue-resident-progenitor-lineages-drive-distinct-phenotypes-of-heterotopic-ossification
#19
Devaveena Dey, Jana Bagarova, Sarah J Hatsell, Kelli A Armstrong, Lily Huang, Joerg Ermann, Ashley J Vonner, Yue Shen, Agustin H Mohedas, Arthur Lee, Elisabeth M W Eekhoff, Annelies van Schie, Marie B Demay, Charles Keller, Amy J Wagers, Aris N Economides, Paul B Yu
Fibrodysplasia ossificans progressiva (FOP), a congenital heterotopic ossification (HO) syndrome caused by gain-of-function mutations of bone morphogenetic protein (BMP) type I receptor ACVR1, manifests with progressive ossification of skeletal muscles, tendons, ligaments, and joints. In this disease, HO can occur in discrete flares, often triggered by injury or inflammation, or may progress incrementally without identified triggers. Mice harboring an Acvr1(R206H) knock-in allele recapitulate the phenotypic spectrum of FOP, including injury-responsive intramuscular HO and spontaneous articular, tendon, and ligament ossification...
November 23, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27879591/comparison-of-endothelial-differentiation-capacities-of-human-and-rat-adipose-derived-stem-cells
#20
Hakan Orbay, Kamaljit Devi, Priscilla A Williams, Tima Dehghani, Eduardo A Silva, David E Sahar
BACKGROUND: The authors compared the endothelial differentiation capacities of human and rat adipose-derived stem cells to determine whether human adipose-derived stem cells can be a source of endothelial cells clinically. METHODS: Human and rat adipose-derived stem cells were harvested and characterized with flow cytometry and trilineage differentiation. Cells from passages III through V were fed with endothelial cell differentiation medium for up to 3 weeks. Cells were harvested after 1, 2, and 3 weeks, and endothelial differentiation was evaluated with quantitative reverse-transcriptase polymerase chain reaction, flow cytometry, and angiogenic sprouting assays...
December 2016: Plastic and Reconstructive Surgery
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