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https://www.readbyqxmd.com/read/28935951/overexpression-of-annexin-gene-annsp2-enhances-drought-and-salt-tolerance-through-modulation-of-aba-synthesis-and-scavenging-ros-in-tomato
#1
Raina Ijaz, Javeria Ejaz, Shenghua Gao, Tengfei Liu, Muhammad Imtiaz, Zhibiao Ye, Taotao Wang
Drought and high salinity are two major abiotic stresses that significantly affect agricultural crop productivity worldwide. Annexins are a multigene family that plays an essential role in plant stress responses and various cellular processes. Here, the AnnSp2 gene was cloned from drought-resistant wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato. AnnSp2 protein was localized in the nucleus and had higher expression in leave, flower and fruit. It was induced by several phytohormones and some abiotic stresses...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935260/insights-of-lr28-mediated-wheat-leaf-rust-resistance-transcriptomic-approach
#2
Dharmendra Singh, Dhananjay Kumar, Lopamudra Satapathy, Jyoti Pathak, Saket Chandra, Adnan Riaz, Govindraj Bhaganagre, Raman Dhariwal, Manish Kumar, Kumble Vinod Prabhu, Harindra Singh Balyan, Pushpendra Kumar Gupta, Kunal Mukhopadhyay
Leaf rust is a fungal disease that causes severe yield losses in wheat. Resistant varieties with major and quantitative resistance genes are the most effective method to control the disease. However, the main problem is inadequate information for understanding resistance mechanism and its usefulness. This paper presents Lr28 mediated genome-wide response of known and unknown genes during wheat-Puccinia triticina interaction. In this study, we prepared Serial Analysis of Gene Expression (SAGE) libraries using seedling wheat mRNA for infected and mock conditions...
September 18, 2017: Gene
https://www.readbyqxmd.com/read/28934710/calcium-availability-but-not-its-content-modulates-metal-toxicity-in-scenedesmus-quadricauda
#3
Jozef Kováčik, Sławomir Dresler
Impact of calcium nutrition (pre-culture on solid medium with standard or elevated Ca dose, i. e. 0.17 and 4.40mM marked as low and high Ca) on acute metal toxicity (Cd, Mn and Pb, 24h of exposure to 10µM) in freshwater green alga Scenedesmus quadricauda was studied. Surprisingly, Ca content differed only slightly between low and high Ca samples and applied metals rather suppressed its amount. Na content was higher in metal-exposed high Ca samples, indicating that Ca/Na ratio may affect accumulation of metals...
September 18, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28930225/vochysia-rufa-stem-bark-extract-protects-endothelial-cells-against-high-glucose-damage
#4
Neire Moura de Gouveia, Sonia Ramos, María Ángeles Martín, Foued Salmen Espindola, Luis Goya, Olga M Palomino
Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated. Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum...
February 21, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28928512/determination-of-antioxidant-activity-of-hibiscus-sabdariffa-and-croton-caudatus-in-saccharomyces-cerevisiae-model-system
#5
Pattnaik Subhaswaraj, M Sowmya, V Bhavana, Madhu Dyavaiah, Busi Siddhardha
From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of Hibiscus sabdariffa and Croton caudatus were evaluated for free radical scavenging activity in Saccharomyces cerevisiae model system. H. sabdariffa and C. caudatus showed tremendous DPPH free radical scavenging potential with an IC50 value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28926923/involvement-of-mapk-nf-%C3%AE%C2%BAb-signal-transduction-pathways-camellia-japonica-mitigates-inflammation-and-gastric-ulcer
#6
Md Rashedunnabi Akanda, Byung-Yong Park
Gastric ulcer is an important risk factor for human health globally. Camellia japonica (CJ) is a plant of which the fruits are used as traditional phytomedicine for inflammatory and immunomodulatory diseases; however, the underlying molecular mechanism has not been clarified. The present study aimed to investigate the immunopharmacological activities of Camellia japonica and validate its pharmacological targets. To evaluate the protective roles of Camellia japonica on LPS-induced inflammation in RAW 264.7 cells and HCl/EtOH-induced gastric ulcer in mice; we applied 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), nitric oxide (NO), reactive oxygen species (ROS), histopathology, malondialdehyde (MDA), quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry (IHC), and western blot analyses...
September 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28925545/methane-delays-the-senescence-and-browning-in-daylily-buds-by-re-established-redox-homeostasis
#7
Huali Hu, Dan Liu, Pengxia Li
BACKGROUND: During the postharvest senescence of fruits and vegetables, redox imbalance occurs. Although the release of methane (CH4 ) has been observed for a long time and its antioxidant properties have recently been demonstrated in animals and plants, the corresponding physiological role of CH4 in regulating plant senescence has not yet been elucidated. RESULTS: Here, our results indicate that the postharvest deterioration of daylily buds during storage is greatly ameliorated by the exogenous application of CH4 , particularly in the inhibition of tissue browning...
September 19, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28924722/evaluation-of-heme-oxygenase-1-ho-1-in-cd-and-ni-induced-cytotoxicity-and-crosstalk-with-ros-quenching-enzymes-in-two-to-four-leaf-stage-seedlings-of-vigna-radiata
#8
Lovely Mahawar, Rajesh Kumar, Gyan Singh Shekhawat
Research on heme oxygenase in plants has received consideration in recent years due to its several roles in development, defense, and metabolism during various environmental stresses. In the current investigation, the role of heme oxygenase (HO) 1 was evaluated in reducing heavy metal (Cd and Ni) uptake and alleviating Cd and Ni toxicity effects in the hydroponically grown seedlings of Vigna radiata var. PDM 54. Seedlings were subjected to Cd- and Ni-induced oxidative stress independently at different concentrations ranging from 10 to 100 μM...
September 18, 2017: Protoplasma
https://www.readbyqxmd.com/read/28924387/activation-of-nrf2-attenuates-pulmonary-vascular-remodeling-via-inhibiting-endothelial-to-mesenchymal-transition-an-insight-from-a-plant-polyphenol
#9
Yucai Chen, Tianyi Yuan, Huifang Zhang, Yu Yan, Danshu Wang, Lianhua Fang, Yang Lu, Guanhua Du
The endothelial-to-mesenchymal transition (EndMT) has been demonstrated to be involved in pulmonary vascular remodeling. It is partly attributed to oxidative and inflammatory stresses in endothelial cells. In current study, we conducted a series of experiments to clarify the effect of salvianolic acid A (SAA), a kind of polyphenol compound, in the process of EndMT in human pulmonary arterial endothelial cells and in vivo therapeutic efficacy on vascular remodeling in monocrotaline (MCT)-induced EndMT. EndMT was induced by TGFβ1 in human pulmonary arterial endothelial cells (HPAECs)...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28922768/accerbatin-a-small-molecule-at-the-intersection-of-auxin-and-reactive-oxygen-species-homeostasis-with-herbicidal-properties
#10
Yuming Hu, Thomas Depaepe, Dajo Smet, Klara Hoyerova, Petr Klíma, Ann Cuypers, Sean Cutler, Dieter Buyst, Kris Morreel, Wout Boerjan, José Martins, Jan Petrášek, Filip Vandenbussche, Dominique Van Der Straeten
The volatile two-carbon hormone ethylene acts in concert with an array of signals to affect etiolated seedling development. From a chemical screen, we isolated a quinoline carboxamide designated ACCERBATIN (AEX) that exacerbates the 1-aminocyclopropane-1-carboxylic acid-induced triple response, typical for ethylene-treated seedlings in darkness. Phenotypic analyses revealed distinct AEX effects including inhibition of root hair development and shortening of the root meristem. Mutant analysis and reporter studies further suggested that AEX most probably acts in parallel to ethylene signaling...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28919898/chitosan-increases-tomato-root-colonization-by-pochonia-chlamydosporia-and-their-combination-reduces-root-knot-nematode-damage
#11
Nuria Escudero, Federico Lopez-Moya, Zahra Ghahremani, Ernesto A Zavala-Gonzalez, Aurora Alaguero-Cordovilla, Caridad Ros-Ibañez, Alfredo Lacasa, Francisco J Sorribas, Luis V Lopez-Llorca
The use of biological control agents could be a non-chemical alternative for management of Meloidogyne spp. [root-knot nematodes (RKN)], the most damaging plant-parasitic nematodes for horticultural crops worldwide. Pochonia chlamydosporia is a fungal parasite of RKN eggs that can colonize endophytically roots of several cultivated plant species, but in field applications the fungus shows a low persistence and efficiency in RKN management. The combined use of P. chlamydosporia with an enhancer could help its ability to develop in soil and colonize roots, thereby increasing its efficiency against nematodes...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28918123/carnosic-acid-induces-apoptosis-of-hepatocellular-carcinoma-cells-via-ros-mediated-mitochondrial-pathway
#12
Xinrui Zhang, Yiling Chen, Guangsheng Cai, Xin Li, Di Wang
Carnosic acid (CA), an important bioactive phenolic diterpene mainly found in labiate plants, exerts various biological functions, including antioxidant, anti-inflammatory, antitumor, and neuroprotective activities. In the present study, we proved the deleterious effects of CA against hepatocellular carcinoma (HCC) in both in vitro and in vivo models. In vitro, CA significantly decreased cell viability, inhibited cell proliferation and migration, enhanced apoptosis, and increased caspase-3, -8, and -9 activities in HepG2 and SMMC-7721 cells...
September 13, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28916593/carnosic-acid-and-carnosol-two-major-antioxidants-of-rosemary-act-through-different-mechanisms
#13
Margot Loussouarn, Anja Krieger-Liszkay, Ljubica Svilar, Antoine Bily, Simona Birtic, Michel Havaux
Carnosic acid, a phenolic diterpene specific of the Lamiaceae family, is highly abundant in rosemary species. Despite numerous industrial and medicinal/pharmaceutical applications of its antioxidative features, this compound in planta and its antioxidant mechanism have received little attention, except a few studies of rosemary plants under natural conditions. In vitro analyses, using HPLC-UV and luminescence imaging, revealed that carnosic acid and its major oxidized derivative, carnosol, protect lipids from oxidation...
September 15, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28915644/phytoagent-deoxyelephantopin-derivative-inhibits-triple-negative-breast-cancer-cell-activity-by-inducing-oxidative-stress-mediated-paraptosis-like-cell-death
#14
Jeng-Yuan Shiau, Kyoko Nakagawa-Goto, Kuo-Hsiung Lee, Lie-Fen Shyur
Triple negative breast cancer (TNBC) is a highly metastatic cancer among the breast cancer subgroups. A thorny issue for clinical therapy of TNBC is lack of an efficient targeted therapeutic strategy. We previously created a novel sesquiterpene lactone analog (named DETD-35) derived from plant deoxyelephantopin (DET) which exhibits potent effects against human TNBC MDA-MB-231 tumor growth in a xenograft mouse model. Here we studied the mechanisms of both DET and DETD-35 against MDA-MB-231 cells. DETD-35 (3-fold decreased in IC50) exhibited better anti-TNBC cell activity than DET as observed through induction of reactive oxygen species production (within 2 h treatment) and damage to the ER structures, resulting in ER-derived cytoplasmic vacuolation and ubiquitinated protein accumulation in the treated cells...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28913895/nramp2-a-trans-golgi-network-localized-manganese-transporter-is-required-for-arabidopsis-root-growth-under-manganese-deficiency
#15
Huiling Gao, Wenxiang Xie, Changhong Yang, Jingyi Xu, Jingjun Li, Hua Wang, Xi Chen, Chao-Feng Huang
To cope with manganese (Mn) deficiency, plants have evolved an efficient transport system to uptake and redistribute Mn. However, the underlying molecular mechanisms remain to be demonstrated. We carried out a forward genetic screen in a root high-affinity Mn transporter nramp1 mutant background in Arabidopsis thaliana and identified an uncharacterized Mn transport NRAMP2. We investigated the effect of nramp2 mutation on root growth and reactive oxygen species (ROS) accumulation and we also examined the NRAMP2 expression pattern, and the subcellular localization and transport activity of NRAMP2...
September 15, 2017: New Phytologist
https://www.readbyqxmd.com/read/28906105/enantioselective-phytotoxicity-of-dichlorprop-to-arabidopsis-thaliana-effect-of-cytochrome-p450-enzymes-and-role-of-fe
#16
Zunwei Chen, Jia Wang, Hui Chen, Yuezhong Wen, Weiping Liu
The ecotoxicology effects of chiral herbicides have long been recognized and have drawn increasing attention. The toxic mechanisms of herbicides in plants are involved in production of reactive oxygen species (ROS) and cause damage to target enzyme, but the relationship between these two factors in the enantioselectivity of chiral herbicides has rarely been investigated. Furthermore, even though cytochromes P450 enzymes (CYP450s) have been related to the phytotoxicity of herbicides, their roles in the enantioselectivity of chiral herbicides have yet to be explored...
September 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28906064/reactive-oxygen-species-generated-in-chloroplasts-contribute-to-tobacco-leaf-infection-by-the-necrotrophic-fungus-botrytis-cinerea
#17
Franco R Rossi, Adriana R Krapp, Fabiana Bisaro, Santiago J Maiale, Fernando L Pieckenstain, Néstor Carrillo
Reactive oxygen species (ROS) play fundamental roles in plant responses to pathogen infection, including modulation of cell death processes and defense-related gene expression. Cell death triggered as part of the hypersensitive response enhances resistance to biotrophic pathogens, but favors virulence of necrotrophs. Even though the involvement of ROS in the orchestration of defense responses is well established, the relative contribution of specific subcellular ROS sources to plant resistance against microorganisms with different pathogenesis strategies is not completely known...
September 14, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28902851/redox-mediated-quorum-sensing-in-plants
#18
Alexandra W Fuller, Phoebe Young, B Daniel Pierce, Jamie Kitson-Finuff, Purvi Jain, Karl Schneider, Stephen Lazar, Olga Taran, Andrew G Palmer, David G Lynn
The rhizosphere, the narrow zone of soil around plant roots, is a complex network of interactions between plants, bacteria, and a variety of other organisms. The absolute dependence on host-derived signals, or xenognosins, to regulate critical developmental checkpoints for host commitment in the obligate parasitic plants provides a window into the rhizosphere's chemical dynamics. These sessile intruders use H2O2 in a process known as semagenesis to chemically modify the mature root surfaces of proximal host plants and generate p-benzoquinones (BQs)...
2017: PloS One
https://www.readbyqxmd.com/read/28902159/exogenous-melatonin-alleviates-alkaline-stress-in-malus-hupehensis-rehd-by-regulating-the-biosynthesis-of-polyamines
#19
Xiaoqing Gong, Shuting Shi, Fangfang Dou, Yi Song, Fengwang Ma
Since melatonin was identified in plants decades ago, much attention has been devoted to discovering its role in plant science. There is still a great deal to learn about the functional importance of melatonin, as well as its functional mode. In this paper, we examine the role of melatonin treatment in the response of Malus hupehensis Rehd. to alkaline conditions. Stressed seedlings showed chlorosis and suppressed growth. However, this phenotype was ameliorated when 5 µM melatonin was added to the irrigation solution...
September 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28895264/phenolic-compound-diversity-explored-in-the-context-of-photo-oxidative-stress-protection
#20
Kristóf Csepregi, Éva Hideg
INTRODUCTION: Phenolic compounds are a chemically diverse group of plant secondary metabolites with important roles both in plant stress defence and human nutrition. OBJECTIVE: To explore structure-function relations potentiating phenolic compounds to promote leaf acclimation to light stress by excess photosynthetically active radiation (photoinhibition) and by solar ultraviolet (UV) radiation. METHODOLOGY: We report singlet oxygen and hydrogen peroxide antioxidant capacities and UV-absorbing properties of 27 flavonoids and 11 phenolic acids...
September 12, 2017: Phytochemical Analysis: PCA
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