keyword
https://read.qxmd.com/read/37361547/nitrogen-regulated-antisense-transcription-in-the-adaptation-to-nitrogen-deficiency-in-nostoc-sp-pcc-7120
#21
JOURNAL ARTICLE
Manuel Brenes-Álvarez, Agustín Vioque, Alicia M Muro-Pastor
Transcriptomic analyses using high-throughput methods have revealed abundant antisense transcription in bacteria. Antisense transcription is often due to the overlap of mRNAs with long 5' or 3' regions that extend beyond the coding sequence. In addition, antisense RNAs that do not contain any coding sequence are also observed. Nostoc sp. PCC 7120 is a filamentous cyanobacterium that, under nitrogen limitation, behaves as a multicellular organism with division of labor among two different cell types that depend on each other, the vegetative CO2 -fixing cells and the nitrogen-fixing heterocysts...
June 2023: PNAS Nexus
https://read.qxmd.com/read/37322092/global-translational-control-by-the-transcriptional-repressor-trcr-in-the-filamentous-cyanobacterium-anabaena-sp-pcc-7120
#22
JOURNAL ARTICLE
Zi-Qian Wang, Yiling Yang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Transcriptional and translational regulations are important mechanisms for cell adaptation to environmental conditions. In addition to house-keeping tRNAs, the genome of the filamentous cyanobacterium Anabaena sp. strain PCC 7120 (Anabaena) has a long tRNA operon (trn operon) consisting of 26 genes present on a megaplasmid. The trn operon is repressed under standard culture conditions, but is activated under translational stress in the presence of antibiotics targeting translation. Using the toxic amino acid analog β-N-methylamino-L-alanine (BMAA) as a tool, we isolated and characterized several BMAA-resistance mutants from Anabaena, and identified one gene of unknown function, all0854, named as trcR, encoding a transcription factor belonging to the ribbon-helix-helix (RHH) family...
June 15, 2023: Communications Biology
https://read.qxmd.com/read/37321385/tight-association-of-cpcl-with-photosystem-i-in-anabaena-sp-pcc-7120-grown-under-iron-deficient-conditions
#23
JOURNAL ARTICLE
Shota Shimizu, Haruya Ogawa, Naoki Tsuboshita, Takehiro Suzuki, Koji Kato, Yoshiki Nakajima, Naoshi Dohmae, Jian-Ren Shen, Ryo Nagao
Phycobilisomes (PBSs), which are huge pigment-protein complexes displaying distinctive color variations, bind to photosystem cores for excitation-energy transfer. It is known that the isolation of supercomplexes consisting of PBSs and photosystem I (PSI) or PBSs and photosystem II (PSII) is challenging due to weak interactions between PBSs and the photosystem cores. In this study, we succeeded in purifying PSI-monomer-PBS and PSI-dimer-PBS supercomplexes from the cyanobacterium Anabaena sp. PCC 7120 grown under iron-deficient conditions by anion-exchange chromatography, followed by trehalose density gradient centrifugation...
June 13, 2023: Biochimica et Biophysica Acta. Bioenergetics
https://read.qxmd.com/read/37277048/effects-of-dietary-supplementation-of-anabaena-sp-pcc7120-expressing-vp28-protein-on-survival-and-histopathology-after-wssv-infection-in-macrobrachium-nipponense
#24
JOURNAL ARTICLE
Yupei Tong, Jia Yang, Li Wang, Xiaoping Chi, Chan Zhu, Rong Yin, Le Zhang, Yaru Li, Chunyan Zhao, Rui Jia
Shrimp are especially susceptible to the White Spot Syndrome Virus (WSSV). Oral administration of the WSSV envelop protein VP28 is a promising approach to protect shrimp against WSSV. In this study, Macrobrachium nipponense (M. nipponense) were fed for 7 days with food supplemented with Anabaena sp. PCC 7120 (Ana7120) expressing VP28 and then challenged with WSSV. The survival rates of M. nipponense in three groups, including control, WSSV-challenged, and VP28-vaccinated, were subsequently determined. We also determined the WSSV content of different tissues and the tissue morphology in the absence of and after viral challenge...
June 3, 2023: Fish & Shellfish Immunology
https://read.qxmd.com/read/37152674/acylated-plastoquinone-is-a-novel-neutral-lipid-accumulated-in-cyanobacteria
#25
JOURNAL ARTICLE
Toshiki Ishikawa, Shunya Takano, Riko Tanikawa, Takashi Fujihara, Kimie Atsuzawa, Yasuko Kaneko, Yukako Hihara
Although cyanobacteria do not possess bacterial triacylglycerol (TAG)-synthesizing enzymes, the accumulation of TAGs and/or lipid droplets has been repeatedly reported in a wide range of species. In most cases, the identification of TAG has been based on the detection of the spot showing the mobility similar to the TAG standard in thin-layer chromatography (TLC) of neutral lipids. In this study, we identified monoacyl plastoquinol (acyl PQH) as the predominant molecular species in the TAG-like spot from the unicellular Synechocystis sp...
May 2023: PNAS Nexus
https://read.qxmd.com/read/37125074/raman-spectral-analysis-of-microbial-pigment-compositions-in-vegetative-cells-and-heterocysts-of-multicellular-cyanobacterium
#26
JOURNAL ARTICLE
Jun-Ichi Ishihara, Hiroki Takahashi
The one-dimensional multicellular cyanobacterium, Anabaena sp. PCC 7120, exhibits a simple topology consisting of two types of cells under the nitrogen-depleted conditions. Although the differentiated (heterocyst) and undifferentiated cells (vegetative cells) were distinguished by their cellular shapes, we found that their internal states, that is, microbial pigment compositions, were distinguished by using a Raman microscope. Almost of Raman bands of the cellular components were assigned to vibrations of the pigments; chlorophyll a , β-carotene, phycocyanin, and allophycocyanin...
July 2023: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/37032853/characterization-of-a-cyanobacterial-rep-protein-with-broad-host-range-and-its-utilization-for-expression-vectors
#27
JOURNAL ARTICLE
Yutaka Sakamaki, Kaisei Maeda, Kaori Nimura-Matsune, Taku Chibazakura, Satoru Watanabe
Owing to their photosynthetic capabilities, cyanobacteria are regarded as ecologically friendly hosts for production of biomaterials. However, compared to other bacteria, tools for genetic engineering, especially expression vector systems, are limited. In this study, we characterized a Rep protein, exhibiting replication activity in multiple cyanobacteria and established an expression vector using this protein. Our comprehensive screening using a genomic library of Synechocystis sp. PCC 6803 revealed that a certain region encoding a Rep-related protein (here named Cyanobacterial Rep protein A2: CyRepA2) exhibits high autonomous replication activity in a heterologous host cyanobacterium, Synechococcus elongatus PCC 7942...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/36884198/citrate-synthase-from-cyanidioschyzon-merolae-exhibits-high-oxaloacetate-and-acetyl-coa-catalytic-efficiency
#28
JOURNAL ARTICLE
Maki Nishii, Shoki Ito, Takashi Osanai
Citrate synthase (CS) catalyzes the reaction that produces citrate and CoA from oxaloacetate and acetyl-CoA in the tricarboxylic acid (TCA) cycle. All TCA cycle enzymes are localized to the mitochondria in the model organism, the red alga Cyanidioschyzon merolae. The biochemical properties of CS have been studied in some eukaryotes, but the biochemical properties of CS in algae, including C. merolae, have not been studied. We then performed the biochemical analysis of CS from C. merolae mitochondria (CmCS4)...
March 8, 2023: Plant Molecular Biology
https://read.qxmd.com/read/36859522/the-organization-of-the-phycobilisome-photosystem-i-supercomplex-depends-on-the-ratio-between-two-different-phycobilisome-linker-proteins
#29
JOURNAL ARTICLE
Mai Watanabe, Masahiko Ikeuchi, Annegret Wilde
The phycobilisome (PBS) is an antenna protein complex in cyanobacteria, Glaucocystophytes, and red algae. In the standard PBS, the rod-core PBS, the rods are connected to the core by the rod-core linker protein CpcG. The rod-core PBS transfers the light energy mainly to photosystem (PS) II and to a lesser extent to PSI. Cyanobacteria assemble another type of PBS, the CpcL-PBS, which consists of only one rod. This rod-type PBS is connected to the thylakoid membrane by the linker protein CpcL and is a PSI-specific antenna...
March 1, 2023: Photochemical & Photobiological Sciences
https://read.qxmd.com/read/36805598/structure-of-a-monomeric-photosystem-i-core-associated-with-iron-stress-induced-a-proteins-from-anabaena-sp-pcc-7120
#30
JOURNAL ARTICLE
Ryo Nagao, Koji Kato, Tasuku Hamaguchi, Yoshifumi Ueno, Naoki Tsuboshita, Shota Shimizu, Miyu Furutani, Shigeki Ehira, Yoshiki Nakajima, Keisuke Kawakami, Takehiro Suzuki, Naoshi Dohmae, Seiji Akimoto, Koji Yonekura, Jian-Ren Shen
Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions. The cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, their binding property and functional roles in PSI are still missing. We analyzed a cryo-electron microscopy structure of a PSI-IsiA supercomplex isolated from Anabaena grown under an iron-deficient condition. The PSI-IsiA structure contains six IsiA subunits associated with the PsaA side of a PSI core monomer. Three of the six IsiA subunits were identified as IsiA1 and IsiA2...
February 17, 2023: Nature Communications
https://read.qxmd.com/read/36801863/cvkr-is-a-merr-type-transcriptional-repressor-of-class-2-type-v-k-crispr-associated-transposase-systems
#31
JOURNAL ARTICLE
Marcus Ziemann, Viktoria Reimann, Yajing Liang, Yue Shi, Honglei Ma, Yuman Xie, Hui Li, Tao Zhu, Xuefeng Lu, Wolfgang R Hess
Certain CRISPR-Cas elements integrate into Tn7-like transposons, forming CRISPR-associated transposon (CAST) systems. How the activity of these systems is controlled in situ has remained largely unknown. Here we characterize the MerR-type transcriptional regulator Alr3614 that is encoded by one of the CAST (AnCAST) system genes in the genome of cyanobacterium Anabaena sp. PCC 7120. We identify a number of Alr3614 homologs across cyanobacteria and suggest naming these regulators CvkR for Cas V-K repressors. Alr3614/CvkR is translated from leaderless mRNA and represses the AnCAST core modules cas12k and tnsB directly, and indirectly the abundance of the tracr-CRISPR RNA...
February 18, 2023: Nature Communications
https://read.qxmd.com/read/36625639/control-of-cell-size-by-c-di-gmp-requires-a-two-component-signaling-system-in-the-cyanobacterium-anabaena-sp-strain-pcc-7120
#32
JOURNAL ARTICLE
Qin-Xue Sun, Min Huang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Each bacterial species possesses a specific cell size and morphology, which constitute important and recognizable physical traits. How bacteria maintain their particular cell size and morphology remains an essential question in microbiology. Cyanobacteria are oxygen-evolving photosynthetic prokaryotes. Although monophyletic, these organisms are highly diverse in their cell morphology and cell size. How these physical traits of cyanobacteria are controlled is poorly understood. Here, we report the identification of a two-component signaling system, composed of a histidine kinase CdgK and a response regulator CdgS, involved in cell size regulation in the filamentous, heterocyst-forming cyanobacterium Anabaena sp...
January 10, 2023: Microbiology Spectrum
https://read.qxmd.com/read/36572159/pretreatment-of-cyanobacterial-biomass-for-the-production-of-biofuel-in-microbial-fuel-cells
#33
REVIEW
Soumya Pandit, Minaxi Sharma, Srijoni Banerjee, Bikram Kumar Nayak, Debabrata Das, Santimoy Khilari, Ram Prasad
The present study delves into phototrophic cyanobacterial biomass production by concomitant CO2 sequestration, selecting an effective pretreatment condition followed by using this as feedstock for green fuel or bioelectricity production by Microbial Fuel Cells (MFC). The performance of the various photobioreactors were put up against Anabaena sp. PCC 7120 biomass production. Maximum algal biomass of 1.15 gL-1 was attained in an airlift bioreactor for 9 days under a light intensity of 100 µEm-2 s-1 . Pretreatment methods like sonication, HCl, and H2 O2 treatment (2% vv-1 ) were applied to digest harvested biomass...
December 23, 2022: Bioresource Technology
https://read.qxmd.com/read/36435857/fixing-n-2-into-cyanophycin-continuous-cultivation-of-nostoc-sp-pcc-7120
#34
JOURNAL ARTICLE
Giulia Trentin, Francesca Piazza, Marta Carletti, Boris Zorin, Inna Khozin-Goldberg, Alberto Bertucco, Eleonora Sforza
Two diazotrophic cyanobacteria (Anabaena cylindrica PCC 7122 and Nostoc sp. PCC 7120) were cultivated to produce cyanophycin, a nitrogen reserve compound, under nitrogen fixing conditions. In preliminary continuous experiments, Nostoc sp. was shown to be more efficient, accumulating a higher amount of cyanophycin and showing a greater capability to fix atmospheric nitrogen in the biomass (67 mgN d-1 of fixed nitrogen per liter of culture). The operating conditions were then optimized to maximize the cyanophycin productivity: the effect of incident light intensity, residence time and nitrogen availability were investigated...
November 26, 2022: Applied Microbiology and Biotechnology
https://read.qxmd.com/read/36143472/the-role-of-mreb-mrec-and-mred-in-the-morphology-of-the-diazotrophic-filament-of-anabaena-sp-pcc-7120
#35
JOURNAL ARTICLE
Cristina Velázquez-Suárez, Ignacio Luque, Antonia Herrero
The cyanobacterium Anabaena sp. PCC 7120 forms filaments of communicating cells. Under conditions of nitrogen scarcity, some cells differentiate into heterocysts, allowing the oxygen-sensitive N2 -reduction system to be expressed and operated in oxic environments. The key to diazotrophic growth is the exchange of molecules with nutritional and signaling functions between the two types of cells of the filament. During heterocyst differentiation, the peptidoglycan sacculus grows to allow cell enlargement, and the intercellular septa are rebuilt to narrow the contact surface with neighboring cells and to hold specific transport systems, including the septal junction complexes for intercellular molecular transfer, which traverse the periplasm between heterocysts and neighboring vegetative cells through peptidoglycan nanopores...
September 15, 2022: Life
https://read.qxmd.com/read/36128660/flv3a-facilitates-o-2-photoreduction-and-affects-h-2-photoproduction-independently-of-flv1a-in-diazotrophic-anabaena-filaments
#36
JOURNAL ARTICLE
Anita Santana-Sánchez, Lauri Nikkanen, Elisa Werner, Gábor Tóth, Maria Ermakova, Sergey Kosourov, Julia Walter, Meilin He, Eva-Mari Aro, Yagut Allahverdiyeva
The model heterocyst-forming filamentous cyanobacterium, Anabaena sp. PCC 7120 (Anabaena) represents multicellular organisms capable of simultaneously performing oxygenic photosynthesis in vegetative cells and O2 -sensitive N2 -fixation inside heterocysts. The flavodiiron proteins (FDPs) have been shown to participate in photoprotection of photosynthesis by driving excess electrons to O2 (Mehler-like reaction). Here, we performed a phenotypic and biophysical characterization of Anabaena mutants impaired in vegetative-specific Flv1A and Flv3A in order to address their physiological relevance on the bioenergetic processes occurring in diazotrophic Anabaena under variable CO2 ...
September 20, 2022: New Phytologist
https://read.qxmd.com/read/36123352/structural-insights-into-cyanobacterial-rubisco-assembly-coordinated-by-two-chaperones-raf1-and-rbcx
#37
JOURNAL ARTICLE
Qiong Li, Yong-Liang Jiang, Ling-Yun Xia, Yuxing Chen, Cong-Zhao Zhou
RuBisCO is the most abundant enzyme in nature, catalyzing the fixation of CO2 in photosynthesis. Its common form consists of eight RbcL and eight RbcS subunits, the assembly of which requires a series of chaperones that include RbcX and RuBisCO accumulation factor 1 (Raf1). To understand how these RuBisCO-specific chaperones function during cyanobacterial RbcL8 RbcS8 (L8 S8 ) holoenzyme formation, we solved a 3.3-Å cryo-electron microscopy structure of a 32-subunit RbcL8 Raf18 RbcX16 (L8 F8 X16 ) assembly intermediate from Anabaena sp...
September 20, 2022: Cell Discovery
https://read.qxmd.com/read/36054741/the-two-component-response-regulator-orra-confers-dehydration-tolerance-by-regulating-avaka-expression-in-the-cyanobacterium-anabaena-sp-strain-pcc-7120
#38
JOURNAL ARTICLE
Satoshi Kimura, Miho Sato, Xingyan Fan, Masayuki Ohmori, Shigeki Ehira
The cyanobacterium Anabaena sp. strain PCC 7120 exhibits dehydration tolerance. The regulation of gene expression in response to dehydration is crucial for the acquisition of dehydration tolerance, but the molecular mechanisms underlying dehydration responses remain unknown. In this study, the functions of the response regulator OrrA in the regulation of salt and dehydration responses were investigated. Disruption of orrA abolished or diminished the induction of hundreds of genes in response to salt stress and dehydration...
August 16, 2022: Environmental Microbiology
https://read.qxmd.com/read/36037363/a-proteolytic-pathway-coordinates-cell-division-and-heterocyst-differentiation-in-the-cyanobacterium-anabaena-sp-pcc-7120
#39
JOURNAL ARTICLE
Wei-Yue Xing, Jing Liu, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
The filamentous, multicellular cyanobacterium Anabaena sp. PCC 7120 ( Anabaena ) is a prokaryotic model for the study of cell differentiation and cell-cell interactions. Upon combined-nitrogen deprivation, Anabaena forms a particular cell type, heterocyst, for aerobic nitrogen fixation. Heterocysts are semiregularly spaced among vegetative cells. Heterocyst differentiation is coupled to cell division, but the underlying mechanism remains unclear. This mechanism could be mediated by the putative protease HetF, which is a divisome component and is necessary for heterocyst differentiation...
September 6, 2022: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/36029350/the-molecular-toolset-and-techniques-required-to-build-cyanobacterial-cell-factories
#40
JOURNAL ARTICLE
Franz Opel, Ilka M Axmann, Stephan Klähn
Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis, a solar-driven process which allows them to obtain electrons from water to reduce and finally assimilate carbon dioxide. Consequently, they are in the spotlight of biotechnology as photoautotrophic cell factories to generate a large variety of chemicals and biofuels in a sustainable way. Recent progress in synthetic biology has enlarged the molecular toolset to genetically engineer the metabolism of cyanobacteria, mainly targeting common model strains, such as Synechocystis sp...
2023: Advances in Biochemical Engineering/biotechnology
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