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Anabaena sp.PCC 7120

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https://www.readbyqxmd.com/read/29203647/molecular-basis-for-the-integration-of-environmental-signals-by-furb-from-anabaena-sp-pcc-7120
#1
Violeta C Sein-Echaluce, María Carmen Pallarés, Anabael Lostao, Inmaculada Yruela, Adrián Velázquez-Campoy, M Luisa Peleato, María F Fillat
FUR proteins are among the most important families of transcriptional regulators in prokaryotes, often behaving as global regulators. In the cyanobacterium Anabaena PCC 7120 FurB (Zur) controls zinc and redox homeostasis through the repression of target genes in a zinc-dependent manner. In vitro , nonspecific binding of FurB to DNA elicits protection against oxidative damage and avoids cleavage by DNase I. This study provides for the first time evidence of the influence of redox environment in the interaction of FurB with regulatory zinc and its consequences in FurB-DNA binding affinity...
December 4, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29159995/multiple-abc-glucoside-transporters-mediate-sugar-stimulated-growth-in-the-heterocyst-forming-cyanobacterium-anabaena-sp-strain-pcc-7120
#2
Mercedes Nieves-Morión, Enrique Flores
Cyanobacteria are generally capable of photoautotrophic growth and are widely distributed on Earth. The model filamentous, heterocyst-forming strain Anabaena sp. PCC 7120 has been long considered a strict photoautotroph but is now known to be able to assimilate fructose. We have previously described two components of ABC glucoside uptake transporters from Anabaena that are involved in uptake of the sucrose analog esculin: GlsC (a nucleotide-binding domain subunit [NBD]) and GlsP (a transmembrane component [TMD])...
November 21, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/29143082/spatial-separation-of-photosynthesis-and-ethanol-production-by-cell-type-specific-metabolic-engineering-of-filamentous-cyanobacteria
#3
Shigeki Ehira, Takuto Takeuchi, Akiyoshi Higo
Cyanobacteria, which perform oxygenic photosynthesis, have drawn attention as hosts for the direct production of biofuels and commodity chemicals from CO2 and H2O using light energy. Although cyanobacteria capable of producing diverse chemicals have been generated by metabolic engineering, anaerobic non-photosynthetic culture conditions are often necessary for their production. In this study, we conducted cell type-specific metabolic engineering of the filamentous cyanobacterium Anabaena sp. PCC 7120, which forms a terminally differentiated cell called a heterocyst with a semi-regular spacing of 10-15 cells...
November 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29112727/application-of-crispr-interference-for-metabolic-engineering-of-the-heterocyst-forming-multicellular-cyanobacterium-anabaena-sp-pcc-7120
#4
Akiyoshi Higo, Atsuko Isu, Yuki Fukaya, Shigeki Ehira, Toru Hisabori
Anabaena sp. PCC 7120 (A. 7120) is a heterocyst-forming multicellular cyanobacterium that performs nitrogen fixation. This cyanobacterium has been extensively studied as a model for multicellularity in prokaryotic cells. We have been interested in photosynthetic production of nitrogenous compounds using A. 7120. However, the lack of efficient gene repression tools has limited its usefulness. We originally developed an artificial endogenous gene repression method in this cyanobacterium using small antisense RNA (Higo A...
November 3, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28979840/septal-protein-sepj-from-the-heterocyst-forming-cyanobacterium-anabaena-forms-multimers-and-interacts-with-peptidoglycan
#5
Félix Ramos-León, Vicente Mariscal, Natalia Battchikova, Eva-Mari Aro, Enrique Flores
Heterocyst-forming cyanobacteria grow as filaments that can be hundreds of cells long. Proteinaceous septal junctions provide cell-cell binding and communication functions in the filament. In Anabaena sp. strain PCC 7120, the SepJ protein is important for the formation of septal junctions. SepJ consists of integral membrane and extramembrane sections - the latter including linker and coiled-coil domains. SepJ (predicted MW, 81.3 kDa) solubilized from Anabaena membranes was found in complexes of about 296-334 kDa, suggesting that SepJ forms multimeric complexes...
October 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28929086/role-of-two-cell-wall-amidases-in-septal-junction-and-nanopore-formation-in-the-multicellular-cyanobacterium-anabaena-sp-pcc-7120
#6
Jan Bornikoel, Alejandro Carrión, Qing Fan, Enrique Flores, Karl Forchhammer, Vicente Mariscal, Conrad W Mullineaux, Rebeca Perez, Nadine Silber, C Peter Wolk, Iris Maldener
Filamentous cyanobacteria have developed a strategy to perform incompatible processes in one filament by differentiating specialized cell types, N2-fixing heterocysts and CO2-fixing, photosynthetic, vegetative cells. These bacteria can be considered true multicellular organisms with cells exchanging metabolites and signaling molecules via septal junctions, involving the SepJ and FraCD proteins. Previously, it was shown that the cell wall lytic N-acetylmuramyl-L-alanine amidase, AmiC2, is essential for cell-cell communication in Nostoc punctiforme...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28859322/the-secretion-signal-peptide-of-the-cyanobacterial-extracellular-protein-hesf-is-located-at-its-c-terminus
#7
Steeve Lima, Paulo Oliveira, Paula Tamagnini
Cyanobacteria are photosynthetic prokaryotes, capable of sustaining their growth by converting sunlight into chemical energy by fixing CO2 into organic matter. The cyanobacterium Anabaena sp. PCC 7120 is also capable of fixing atmospheric nitrogen, a metabolic process that occurs in specialized cells, the heterocysts. During the process of heterocyst differentiation, drastic morphological changes occur to prepare the future differentiated cell to accommodate the nitrogen fixation metabolism, which is a highly O2-sensitive process...
August 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28859320/role-of-pats-and-cell-type-on-the-heterocyst-spacing-pattern-in-a-filamentous-branching-cyanobacterium
#8
Laura A Antonaru, Dennis J Nürnberg
Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This paper focuses on the more complex, branching cyanobacterium Mastigocladus laminosus (Stigonematales). Contrary to what has been previously published, a heterocyst spacing pattern is present in M...
August 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28730560/impairment-of-ntca-gene-revealed-its-role-in-regulating-iron-homeostasis-ros-production-and-cellular-phenotype-under-iron-deficiency-in-cyanobacterium-anabaena-sp-pcc-7120
#9
Manish Singh Kaushik, Meenakshi Srivastava, Anumeha Singh, Arun Kumar Mishra
Iron deficiency ends up into several unavoidable consequences including damaging oxidative stress in cyanobacteria. NtcA is a global nitrogen regulator controls wide range of metabolisms in addition to regulation of nitrogen metabolism. In present communication, NtcA based regulation of iron homeostasis, ROS production and cellular phenotype under iron deficiency in Anabaena 7120 has been investigated. NtcA regulates the concentration dependent iron uptake by controlling the expression of furA gene. NtcA also regulated pigment synthesis and phenotypic alterations in Anabaena 7120...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28165619/modulation-of-fatty-acids-and-hydrocarbons-in-anabaena-7120-and-its-ntca-mutant-under-calcium
#10
Savita Singh, Ekta Verma, Balkrishna Tiwari, Niveshika, Arun Kumar Mishra
Calcium being a signaling molecule and mediator of cell response, we examined the modulation in fatty acid and hydrocarbon profiles of wild type cyanobacterium Anabaena sp. PCC 7120 and its ntcA mutant under the influence of different calcium chloride concentrations (0-10 mM). Dynamic modifications in fatty acid and hydrocarbon profile were evident through GC-FID analysis of extracted lipids. In the wild type, increase in CaCl2 (10 mM) resulted in unsaturation of fatty acids (observed in terms of high MUFA/PUFA ratio) while hydrocarbon production was distinctly high in the mutant strain compared to wild type at all tested concentrations...
February 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28159891/an-amidase-is-required-for-proper-intercellular-communication-in-the-filamentous-cyanobacterium-anabaena-sp-pcc-7120
#11
Zhenggao Zheng, Amin Omairi-Nasser, Xiying Li, Chunxia Dong, Yan Lin, Robert Haselkorn, Jindong Zhao
Channels that cross cell walls and connect the cytoplasm of neighboring cells in multicellular cyanobacteria are pivotal for intercellular communication. We find that the product of the gene all1140 of the filamentous cyanobacterium Anabaena sp. PCC 7120 is required for proper channel formation. All1140 encodes an amidase that hydrolyses purified peptidoglycans. An All1140-GFP fusion protein is located at the Z-ring in the periplasmic space during most of the cell cycle. An all1140-null mutant (M40) was unable to grow diazotrophically, and no mature heterocysts were observed in the absence of combined nitrogen...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28117365/allosteric-inhibition-of-phosphoenolpyruvate-carboxylases-is-determined-by-a-single-amino-acid-residue-in-cyanobacteria
#12
Masahiro Takeya, Masami Yokota Hirai, Takashi Osanai
Phosphoenolpyruvate carboxylase (PEPC) is an important enzyme for CO2 fixation and primary metabolism in photosynthetic organisms including cyanobacteria. The kinetics and allosteric regulation of PEPCs have been studied in many organisms, but the biochemical properties of PEPC in the unicellular, non-nitrogen-fixing cyanobacterium Synechocystis sp. PCC 6803 have not been clarified. In this study, biochemical analysis revealed that the optimum pH and temperature of Synechocystis 6803 PEPC proteins were 7.3 and 30 °C, respectively...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28096449/specific-glucoside-transporters-influence-septal-structure-and-function-in-the-filamentous-heterocyst-forming-cyanobacterium-anabaena-sp-strain-pcc-7120
#13
Mercedes Nieves-Morión, Sigal Lechno-Yossef, Rocío López-Igual, José E Frías, Vicente Mariscal, Dennis J Nürnberg, Conrad W Mullineaux, C Peter Wolk, Enrique Flores
When deprived of combined nitrogen, some filamentous cyanobacteria contain two cell types: vegetative cells that fix CO2 through oxygenic photosynthesis and heterocysts that are specialized in N2 fixation. In the diazotrophic filament, the vegetative cells provide the heterocysts with reduced carbon (mainly in the form of sucrose) and heterocysts provide the vegetative cells with combined nitrogen. Septal junctions traverse peptidoglycan through structures known as nanopores and appear to mediate intercellular molecular transfer that can be traced with fluorescent markers, including the sucrose analog esculin (a coumarin glucoside) that is incorporated into the cells...
April 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28064366/increased-heterocyst-frequency-by-patn-disruption-in-anabaena-leads-to-enhanced-photobiological-hydrogen-production-at-high-light-intensity-and-high-cell-density
#14
Hajime Masukawa, Hidehiro Sakurai, Robert P Hausinger, Kazuhito Inoue
The effects of increasing the heterocyst-to-vegetative cell ratio on the nitrogenase-based photobiological hydrogen production by the filamentous heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 were studied. Using the uptake hydrogenase-disrupted mutant (ΔHup) as the parent, a deletion-insertion mutant (PN1) was created in patN, known to be involved in heterocyst pattern formation and leading to multiple singular heterocysts (MSH) in Nostoc punctiforme strain ATCC 29133. The PN1 strain showed heterocyst differentiation but failed to grow in medium free of combined-nitrogen; however, a spontaneous mutant (PN22) was obtained on prolonged incubation of PN1 liquid cultures and was able to grow robustly on N2...
March 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28049144/molecular-diffusion-through-cyanobacterial-septal-junctions
#15
Mercedes Nieves-Morión, Conrad W Mullineaux, Enrique Flores
Heterocyst-forming cyanobacteria grow as filaments in which intercellular molecular exchange takes place. During the differentiation of N2-fixing heterocysts, regulators are transferred between cells. In the diazotrophic filament, vegetative cells that fix CO2 through oxygenic photosynthesis provide the heterocysts with reduced carbon and heterocysts provide the vegetative cells with fixed nitrogen. Intercellular molecular transfer has been traced with fluorescent markers, including calcein, 5-carboxyfluorescein, and the sucrose analogue esculin, which are observed to move down their concentration gradient...
January 3, 2017: MBio
https://www.readbyqxmd.com/read/28011872/functional-significance-of-nadph-thioredoxin-reductase-c-in-the-antioxidant-defense-system-of-cyanobacterium-anabaena-sp-pcc-7120
#16
Shoko Mihara, Keisuke Yoshida, Akiyoshi Higo, Toru Hisabori
The redox regulation system is widely accepted as a crucial mechanism for controlling the activities of various metabolic enzymes. In addition to thioredoxin reductase/thioredoxin cascades, NADPH-thioredoxin reductase C (NTRC), a hybrid protein formed by an NADPH-thioredoxin reductase domain and a thioredoxin (Trx) domain, is present in chloroplasts and in most cyanobacteria species. Although several target proteins and physiological functions of NTRC in chloroplasts have been characterized, little is known about NTRC functions in cyanobacteria...
January 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28000119/overexpression-of-phytochelatin-synthase-pcs-enhances-abiotic-stress-tolerance-by-altering-the-proteome-of-transformed-anabaena-sp-pcc-7120
#17
Neha Chaurasia, Yogesh Mishra, Antra Chatterjee, Ruchi Rai, Shivam Yadav, L C Rai
The present study provides data on the insertion of an extra copy of phytochelatin synthase (alr0975) in Anabaena sp. PCC 7120. The recombinant strain (AnFPN-pcs) compared to wild type showed approximately 22.3% increase in growth rate under UV-B, NaCl, heat, CuCl2, carbofuran, and CdCl2. It also registered 2.25-fold enhanced nitrogenase activity and 5-fold higher phytochelatin production. A comparison of the protein profile of wild type with the recombinant strain revealed that recombinant strain accumulated proteins belonging to the following categories: (i) detoxification (nutrient stress induced DNA binding protein, Mn-SOD, Alr0946 (CalA)), (ii) protein folding and modification (molecular chaperone DnaK, FKBP-type peptidyl-prolyl cis-trans isomerase), (iii) nucleotide and amino acid biosynthesis (dihydroorotase and Ketol-acid reductoisomerase), (iv) photosynthesis and respiration (coproporphyrinogen III oxidase, phycocyanin alpha chain, ferredoxin-NADP(+) reductase), and (v) transport (sugar transport ATP-binding protein)...
July 2017: Protoplasma
https://www.readbyqxmd.com/read/27940385/proteomic-analysis-reveals-contrasting-stress-response-to-uranium-in-two-nitrogen-fixing-anabaena-strains-differentially-tolerant-to-uranium
#18
COMPARATIVE STUDY
Bandita Panda, Bhakti Basu, Celin Acharya, Hema Rajaram, Shree Kumar Apte
Two strains of the nitrogen-fixing cyanobacterium Anabaena, native to Indian paddy fields, displayed differential sensitivity to exposure to uranyl carbonate at neutral pH. Anabaena sp. strain PCC 7120 and Anabaena sp. strain L-31 displayed 50% reduction in survival (LD50 dose), following 3h exposure to 75μM and 200μM uranyl carbonate, respectively. Uranium responsive proteome alterations were visualized by 2D gel electrophoresis, followed by protein identification by MALDI-ToF mass spectrometry. The two strains displayed significant differences in levels of proteins associated with photosynthesis, carbon metabolism, and oxidative stress alleviation, commensurate with their uranium tolerance...
January 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/27924128/exopolysaccharide-production-in-anabaena-sp-pcc-7120-under-different-cacl2-regimes
#19
Savita Singh, Ekta Verma, Niveshika, Balkrishna Tiwari, Arun Kumar Mishra
Influence of various levels of CaCl2 (0, 1, 10 and 100 mM) on exopolysaccharide production has been investigated in the cyanobacterium Anabaena 7120. At the concentration of 1 mM CaCl2, growth was found to be stimulatory while 100 mM was sub lethal for the cyanobacterial cells. Estimation of EPS content revealed that EPS production depends on the concentration of calcium ions in the immediate environment with maximum being at10 mM CaCl2. A possible involvement of alr2882 gene in the process of EPS production was also revealed through qRT-PCR...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27902432/self-replicating-shuttle-vectors-based-on-pans-a-small-endogenous-plasmid-of-the-unicellular-cyanobacterium-synechococcus-elongatus-pcc-7942
#20
You Chen, Arnaud Taton, Michaela Go, Ross E London, Lindsey M Pieper, Susan S Golden, James W Golden
To facilitate development of synthetic biology tools for genetic engineering of cyanobacterial strains, we constructed pANS-derived self-replicating shuttle vectors that are based on the minimal replication element of the Synechococcus elongatus strain PCC 7942 plasmid pANS. To remove the possibility of homologous recombination events between the shuttle plasmids and the native pANS plasmid, the endogenous pANS was cured through plasmid incompatibility-mediated spontaneous loss. A heterologous toxin-antitoxin cassette was incorporated into the shuttle vectors for stable plasmid maintenance in the absence of antibiotic selection...
December 2016: Microbiology
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