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https://www.readbyqxmd.com/read/28738588/maldi-matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-ims-of-skin-aspects-of-sample-preparation
#1
Cristiana Santos de Macedo, David M Anderson, Kevin L Schey
MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) allows molecular analysis of biological materials making possible the identification and localization of molecules in tissues, and has been applied to address many questions on skin pathophysiology, as well as on studies about drug absorption and metabolism. Sample preparation for MALDI IMS is the most important part of the workflow, comprising specimen collection and preservation, tissue embedding, cryosectioning, washing, and matrix application...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738076/the-comparison-of-chca-solvent-compositions-for-improving-lc-maldi-performance-and-its-application-to-study-the-impact-of-aflatoxin-b1-on-the-liver-proteome-of-diabetes-mellitus-type-1-mice
#2
Fuu-Jen Tsai, Shih-Yin Chen, Yu-Ching Liu, Hsin-Yi Liao, Chao-Jung Chen
In nanoflow liquid chromatography-matrix-assisted laser desorption/ionization tandem time-of-flight (nanoLC-MALDI-TOF/TOF) approaches, it is critical to directly apply small amounts of the sample elutes on the sample target using a nanoLC system due to its low flow rate of 200 ~ 300 nl/min. It is recommended to apply a sheath liquid containing a matrix with a several μL/min flow rate at the end of the nanoLC column to ensure a larger co-eluted droplet for more reproducible sample spotting and avoid the laborious task of post-manual matrix spotting...
2017: PloS One
https://www.readbyqxmd.com/read/28737838/improved-bacterial-identification-directly-from-urine-samples-with-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#3
Koichi Kitagawa, Katsumi Shigemura, Ken-Ichiro Onuma, Masako Nishida, Mayu Fujiwara, Saori Kobayashi, Mika Yamasaki, Tatsuya Nakamura, Fukashi Yamamichi, Toshiro Shirakawa, Issei Tokimatsu, Masato Fujisawa
BACKGROUND: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) contributes to rapid identification of pathogens in the clinic but has not yet performed especially well for Gram-positive cocci (GPC) causing complicated urinary tract infection (UTI). The goal of this study was to investigate the possible clinical use of MALDI-TOF MS as a rapid method for bacterial identification directly from urine in complicated UTI. METHODS: MALDI-TOF MS was applied to urine samples gathered from 142 suspected complicated UTI patients in 2015-2017...
July 24, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28731323/analyzing-liposomal-drug-delivery-systems-in-three-dimensional-cell-culture-models-using-maldi-imaging-mass-spectrometry
#4
Jessica K Lukowski, Eric M Weaver, Amanda B Hummon
Cancer chemotherapeutics often fail to reach all diseased cells. To help solve this problem, researchers are investigating novel drug delivery systems. Liposomes are an attractive option due to their low toxicity, high biocompatibility, and potential to carry a large amount of a drug to the tumor site, all while avoiding being eliminated from the body. This study evaluates the penetration of doxorubicin-encased liposomes into three-dimensional cell cultures, or spheroids. Liposomes composed of lipids containing head groups of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cholesterol were created by extrusion...
July 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28730308/tlc-surface-integrity-affects-the-detection-of-alkali-adduct-ions-in-tlc-maldi-analysis
#5
Yonghui Dong, Ruggero Ferrazza, Andrea Anesi, Graziano Guella, Pietro Franceschi
Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730086/peptidome-profiling-of-human-serum-of-uveal-melanoma-patients-based-on-magnetic-bead-fractionation-and-mass-spectrometry
#6
Xiang-Yu Shi, Qing Li, Wen-Bin Wei, Li-Ming Tao
AIM: To find new biomarkers for uveal melanoma (UM) by analyzing the serum peptidome profile. METHODS: Proteomic spectra in patients with UM before and after operation were analyzed and compared with those of healthy controls. Magnetic affinity beads were used to capture serum peptides and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer were used to compile serum peptide profiles. RESULTS: A panel of 49 peptides were differentially expressed between UM patients and controls, of which 33 peptides were of higher intensities in patient group and 16 peptides were of higher intensities in control group...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728930/specific-enrichment-of-glycoproteins-with-polymer-monolith-functionalized-with-glycocluster-grafted-%C3%AE-cyclodextrin
#7
Hai-Jiao Zheng, Jiu-Tong Ma, Wei Feng, Qiong Jia
The low abundance of glycoproteins in complex samples results in the prerequisite role of efficient and selective enrichment of them. In the present work, we designed a new kind of glycosylation poly(hydroxyethyl methacrylate-pentaerythritol triacrylate) monolith functionalized with glycocluster grafted β-cyclodextrin for the enrichment of glycoproteins. The introduced modifiers endowed the monolithic material with enhanced hydrophilicity and surface area, which benefitted to improve the enrichment selectivity and extraction efficiency for glycopeptides...
July 11, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28727449/the-use-of-maldi-ms-combined-with-differential-hydrogen-deuterium-exchange-for-semi-automated-protein-global-conformational-screening
#8
Gregory F Pirrone, Heather Wang, Nicole Canfield, Alexander S Chin, Timothy A Rhodes, Alexey A Makarov
Matrix-assisted laser desorption/ionization (MALDI) coupled with a time-of-flight (TOF) mass-spectrometry (MS) detector is acknowledged to be very useful for analysis of biological molecules. At the same time, hydrogen-deuterium exchange (HDX) is a well-known technique for studying protein higher-order structure. However, coupling MALDI with HDX may be challenging due to undesired back-exchange reactions during analysis. In this report, we survey an approach that utilizes MALDI coupled with automated sample preparation to compare global conformational changes of proteins under different solution conditions using differential HDX...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727417/oxygen-dependent-lipid-profiles-of-3d-cultured-human-chondrocytes-revealed-by-maldi-msi
#9
Brenda Bakker, Gert B Eijkel, Ron M A Heeren, Marcel Karperien, Janine N Post, Berta Cillero Pastor
Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supraphysiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (≤ 8%) promotes the chondrogenic phenotype. Exposure of cells to various oxygen levels alters their lipid metabolism, but detailed studies examining how hypoxia affects lipid metabolism in chondrocytes are lacking. To better understand the chondrocyte's behavior in response to oxygen we cultured 3D pellets of human primary chondrocytes in normoxia (20% oxygen) and hypoxia (2...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28726036/proteolytic-effects-of-gingipains-on-trefoil-factor-family-peptides
#10
Ponlatham Chaiyarit, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul, Barbara Potempa, Jan Potempa
OBJECTIVES: The present study was aimed to determine whether trefoil factor family (TFF) peptides which were generally considered to be resistant to proteolysis could be digested by gingipains, a major proteinases produced by Porphyromonas gingivalis. MATERIALS AND METHODS: Recombinant human TFF1, TFF2, and TFF3 peptides were used as substrates. Gingipains including arginine gingipain (RgpB) and lysine gingipain (Kgp) were used as enzymes. Trypsin was used as a control protease...
July 19, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28724284/inter-alpha-inhibitor-h4-as-a-potential-biomarker-predicting-the-treatment-outcomes-in-patients-with-hepatocellular-carcinoma
#11
Eun-Jung Lee, Seung-Hyun Yang, Kyoung-Jin Kim, Hyejung Cha, Seo Jin Lee, Ji-Hye Kim, Junkyu Song, Kyung-Hee Chun, Jinsil Seong
Purpose: Early prediction of treatment outcomes represents an essential step towards increased treatment efficacy and survival in patients with hepatocellular carcinoma (HCC). In this study, we performed two-dimensional electrophoresis (2-DE) followed by protein profiling to identify biomarkers predictive of therapeutic outcomes in patients with HCC who received liver-directed therapy (LDTx) involving local radiotherapy (RT), and studied the underlying mechanisms of the identified proteins...
July 17, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28722283/comparative-analysis-of-storage-conditions-and-homogenization-methods-for-tick-and-flea-species-for-identification-by-maldi-tof-ms
#12
A Nebbak, B El Hamzaoui, J-M Berenger, I Bitam, D Raoult, L Almeras, P Parola
Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months...
July 19, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28721611/bone-targeting-parathyroid-hormone-conjugates-outperform-unmodified-pth-in-the-anabolic-treatment-of-osteoporosis-in-rats
#13
Yang Yang, Ali Aghazadeh-Habashi, Arash Panahifar, Yuchin Wu, Krishna H Bhandari, Michael R Doschak
Synthetic parathyroid hormone (PTH) is clinically indicated for the treatment of osteoporosis, through its anabolic effects on parathyroid hormone receptors (PTHRs), located on osteoblast cells. However, the bioavailability of PTH for bone cells is restricted by the short half-life of PTH and the widespread distribution of PTHRs in non-skeletal tissues. To impart affinity for mineralized bone surfaces, bisphosphonate (BP)-mediated PTH analogues were synthesized, characterized, and evaluated in vitro and in vivo...
August 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28718648/multi-imaging-of-cytokinin-and-abscisic-acid-on-the-roots-of-rice-oryza-sativa-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#14
Katsuhiro Shiono, Riho Hashizaki, Toyofumi Nakanishi, Tatsuko Sakai, Takushi Yamamoto, Koretsugu Ogata, Ken-Ichi Harada, Hajime Ohtani, Hajime Katano, Shu Taira
Plant hormones are important mobile endogenous signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Visual information on more than one plant hormone from the same sample would be especially powerful, because it could enable rapid, intuitive interpretation. However, multi-imaging techniques for plant hormones have been unavailable. Here, we report the first multi-imaging of two plant hormones, one of cytokinin (i.e. trans-zeatin) and abscisic acid (ABA) by MALDI-TOF-MS imaging...
July 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28716189/prediction-of-streptococcus-uberis-clinical-mastitis-risk-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-in-dairy-herds
#15
Simon C Archer, Andrew J Bradley, Selin Cooper, Peers L Davies, Martin J Green
The purpose of this study was to evaluate whether the risk of Streptococcus uberis clinical mastitis at cow level could be predicted from the historical presence of specific strains of S. uberis on dairy farms. Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to identify S. uberis isolates potentially capable of contagious transmission. Data were available from 10,652 cows from 52 English and Welsh dairy farms over a 14 month period, and 521 isolates of S. uberis from clinical mastitis cases were available for analysis...
September 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28713782/characterization-and-differentiation-of-mycobacterium-avium-subsp-paratuberculosis-from-other-mycobacteria-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#16
Subbarao V Ravva, Leslie A Harden, Chester Z Sarreal
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in cattle, is responsible for significant economic losses to the US dairy industry. The pathogen has also been associated with chronic human diseases like Crohn's disease, type 1 diabetes and multiple sclerosis. Determining causation requires rapid characterization and source tracking the pathogen. Here, we used matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to characterize and differentiate strains of MAP from 14 other species of Mycobacterium from bovine, human, and environmental sources...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28712153/proteomic%C3%A2-analysis-reveals-the-enhancement-of-human-serum-apolipoprotein-a-1-apo-a-1-in%C3%A2-individuals%C3%A2-infected-with-multiple-dengue-virus-serotypes
#17
Manchala Nageswar Reddy, Ranjeet Dungdung, Rajendra Pilankatta
OBJECTIVES: Human serum protein profiling of the individual infected with multiple dengue virus serotypes for identifying the potential biomarkers and to investigate the cause for the severity of dengue virus infection. METHODS: Dengue virus NS1 positive serum samples were pooled into two groups (S2 and S3) based on the molecular serotyping and number of heterotypic infections. The pooled serum samples were subjected to two dimensional gel electrophoresis (2DGE) to identify the differentially expressed proteins...
July 16, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28711436/a-simple-method-for-rapid-microbial-identification-from-positive-monomicrobial-blood-culture-bottles-through-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#18
Jung-Fu Lin, Mao-Cheng Ge, Tsui-Ping Liu, Shih-Cheng Chang, Jang-Jih Lu
BACKGROUND AND PURPOSE: Rapid identification of microbes in the bloodstream is crucial in managing septicemia because of its high disease severity, and direct identification from positive blood culture bottles through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can shorten the turnaround time. Therefore, we developed a simple method for rapid microbiological identification from positive blood cultures by using MALDI-TOF MS. METHODS: We modified previously developed methods to propose a faster, simpler and more economical method, which includes centrifugation and hemolysis...
June 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28707193/laser-pulse-width-dependence-and-ionization-mechanism-of-matrix-assisted-laser-desorption-ionization
#19
Sheng-Ping Liang, I-Chung Lu, Shang-Ting Tsai, Jien-Lian Chen, Yuan Tseh Lee, Chi-Kung Ni
Ultraviolet laser pulses at 355 nm with variable pulse widths in the region from 170 ps to 1.5 ns were used to investigate the ionization mechanism of matrix-assisted laser desorption/ionization (MALDI) for matrices 2,5-dihydroxybenzoic acid (DHB), α-cyano-4-hydroxycinnamic acid (CHCA), and sinapinic acid (SA). The mass spectra of desorbed ions and the intensity and velocity distribution of desorbed neutrals were measured simultaneously for each laser shot. These quantities were found to be independent of the laser pulse width...
July 13, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28706514/evaluation-of-the-bruker-biotyper-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-identification-of-aspergillus-species-directly-from-growth-on-solid-agar-media
#20
Ying Li, He Wang, Yu-Pei Zhao, Ying-Chun Xu, Po-Ren Hsueh
We evaluated the accuracy of the Bruker Biotyper matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) system at identifying clinical isolates of Aspergillus species that were grown on agar media. A total of 381 non-duplicate Aspergillus isolates representing 21 different Aspergillus species identified by molecular analysis were included in this study. The Bruker Biotyper MALDI-TOF MS system was able to identify 30.2% (115/381) of the isolates to the species level (score values of ≥2...
2017: Frontiers in Microbiology
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