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https://www.readbyqxmd.com/read/28453489/changes-of-cerebrospinal-fluid-peptides-due-to-tauopathy
#1
Petra Majerova, Peter Barath, Alena Michalicova, Stanislav Katina, Michal Novak, Andrej Kovac
Alzheimer's disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes...
April 27, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28443326/ionic-liquid-silica-precursors-via-solvent-free-sol-gel-process-and-their-application-in-epoxy-amine-network-a-theoretical-experimental-study
#2
Magdalena Perchacz, Ricardo Keitel Donato, Leandro Seixas, Alexander Zhigunov, Rafal Konefal, Magdalena Serkis-Rodzeń, Hynek Benes
This work describes the solvent-free sol-gel synthesis of epoxy-functionalized silica-based precursors, in the presence of 1-butyl-3-methylimidazolium-based ionic liquids (ILs) containing different anions - chloride (Cl(-)) and methanesulfonate (MeSO3(-)). The IL-driven sol-gel mechanisms were investigated in details using experimental characterizations ((29)Si NMR and ATR FTIR spectroscopy) and a theoretical computational method based on density functional theory (DFT). We observed a complex IL's influence on both hydrolysis and condensation steps, involving especially H-bonding and Coulomb coupling stabilization of the process intermediates...
April 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28441930/ribosomal-subunit-protein-typing-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-the-identification-and-discrimination-of-aspergillus-species
#3
Sayaka Nakamura, Hiroaki Sato, Reiko Tanaka, Yoko Kusuya, Hiroki Takahashi, Takashi Yaguchi
BACKGROUND: Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species...
April 26, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28439630/myriocin-treatment-of-cf-lung-infection-and-inflammation-complex-analyses-for-enigmatic-lipids
#4
Anna Caretti, Michele Vasso, Fabiola Tecla Bonezzi, Andrea Gallina, Marco Trinchera, Alice Rossi, Raffaella Adami, Josefina Casas, Monica Falleni, Delfina Tosi, Alessandra Bragonzi, Riccardo Ghidoni, Cecilia Gelfi, Paola Signorelli
Our aim was to use quantitative and qualitative analyses to gain further insight into the role of ceramide in cystic fibrosis (CF). Sphingolipid ceramide is a known inflammatory mediator, and its accumulation in inflamed lung has been reported in different types of emphysema, chronic obstructive pulmonary disease and CF. CF is caused by a mutation of the chloride channel and associated with hyperinflammation of the respiratory airways and high susceptibility to ongoing infections. We have previously demonstrated that de novo ceramide synthesis is enhanced in lung inflammation and sustains Pseudomonas aeruginosa pulmonary infection in a CF murine model...
April 24, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28438696/altered-temporal-lobe-white-matter-lipid-ion-profiles-in-an-experimental-model-of-sporadic-alzheimer-s-disease
#5
Ming Tong, Raiane Leão, Gina V Vimbela, Emine B Yalcin, Jared Kay, Alexander Krotow, Suzanne M de la Monte
BACKGROUND: White matter is an early and important yet under-evaluated target of Alzheimer's disease (AD). Metabolic impairments due to insulin and insulin-like growth factor resistance contribute to white matter degeneration because corresponding signal transduction pathways maintain oligodendrocyte function and survival. METHODS: This study utilized a model of sporadic AD in which adult Long Evans rats administered intracerebral streptozotocin (i.c. STZ) developed AD-type neurodegeneration...
April 21, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28438498/performance-of-maldi-tof-ms-for-identification-of-oral-prevotella-species
#6
Mervi Gürsoy, Inka Harju, Jaakko Matomäki, Anne Bryk, Eija Könönen
During the past decade, the clinically relevant genus Prevotella has expanded considerably. Prevotella species can be isolated from nearly all types of oral infections but also from various non-oral infections. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introduced in clinical microbiology laboratories as a convenient method for identifying bacterial isolates from clinical specimens. Here we tested the diagnostic accuracy of a total of 123 oral Prevotella isolates, selected based on their biochemical profile, by Bruker MALDI-TOF MS...
April 21, 2017: Anaerobe
https://www.readbyqxmd.com/read/28437719/matrix-assisted-laser-desorption-ionization-mass-spectrometric-imaging-of-olanzapine-in-a-single-hair-using-esculetin-as-a-matrix
#7
Hang Wang, Ying Wang, Ge Wang, Lizhi Hong
Matrix-assisted laser desorption/ionization-mass spectrometric imaging (MALDI-MSI) for the analysis of intact hair is a powerful tool for monitoring changes in drug consumption. The embedding of a low drug concentration in the hydrophobic hair matrix makes it difficult to extract and detect, and requires an improved method to increase detection sensitivity. In this study, an MSI method using MALDI-Fourier transform ion cyclotron resonance was developed for direct identification and imaging of olanzapine in hair samples using the positive ion mode...
April 18, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28436032/application-of-maldi-tof-ms-for-estimating-the-postmortem-interval-in-rat-muscle-samples
#8
Chengzhi Li, Dong Ma, Kaifei Deng, Yijiu Chen, Ping Huang, Zhenyuan Wang
Estimating the postmortem interval (PMI) is very important in the forensic sciences. Although many approaches have been used for estimating the PMI, accurate PMI calculations are still difficult. In this study, four Sprague Dawley (SD) rats were sacrificed by suffocation, and muscle samples were collected by dissection at various time intervals (0, 48, 96, and 144 h) after death. All samples were probed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to obtain molecular images and data for principal component analysis (PCA)...
April 23, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28434974/proteomic-approach-to-detect-changes-in-hippocampal-protein-levels-in-an-animal-model-of-type-2-diabetes
#9
Sung Min Nam, Dae Young Yoo, Hyun Jung Kwon, Jong Whi Kim, Hyo Young Jung, Dae Won Kim, Ho Jae Han, Moo-Ho Won, Je Kyung Seong, In Koo Hwang, Yeo Sung Yoon
In our previous study, we demonstrated that type 2 diabetes affects blood-brain barrier integrity and ultrastructural morphology in Zucker diabetic fatty (ZDF) rats at 40 weeks of age. In the present study, we investigated the possible candidates for diabetes-related proteins in the hippocampus of ZDF rats and their control littermates (Zucker lean control, ZLC), by using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF)...
April 20, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28431114/molecular-characterization-of-cryptococcus-neoformans-isolated-from-the-environment-in-beijing-china
#10
Hongtao Dou, Huizhu Wang, Shaowei Xie, Xinxin Chen, Zhipeng Xu, Yingchun Xu
The molecular type of environmental Cryptococcus neoformans in Beijing was not clear. Our study aims to reveal the molecular characterization of C. neoformans complex from environment in Beijing, China. A total of 435 samples of pigeon droppings from 11 different homes in Beijing were collected from August to November in 2015. Pigeon droppings were inoculated onto caffeic acid cornmeal agar (CACA) to screen C. neoformans complex. Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed for species identification...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28430150/synthesis-and-self-assembly-of-shape-amphiphiles-based-on-poss-dendron-conjugates
#11
Yu Shao, Minyuan Ding, Yujie Xu, Fangjia Zhao, Hui Dai, Xia-Ran Miao, Shuguang Yang, Hui Li
Shape has been increasingly recognized as an important factor for self-assembly. In this paper, a series of shape amphiphiles have been built by linking polyhedral oligomeric silsesquioxane (POSS) and a dendron via linkers of different lengths. Three conjugates of octahedral silsesquioxanes (T₈-POSS) and dendron are designed and synthesized and are referred to as isobutyl T₈-POSS gallic acid derivatives (BPOSS-GAD-1, BPOSS-GAD-2, BPOSS-GAD-3). These samples have been fully characterized by ¹H-NMR, (13)C-NMR, Fourier transform infrared (FT-IR) spectroscopy and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry to establish their chemical identity and purity...
April 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28430138/nanomaterials-as-assisted-matrix-of-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-the-analysis-of-small-molecules
#12
REVIEW
Minghua Lu, Xueqing Yang, Yixin Yang, Peige Qin, Xiuru Wu, Zongwei Cai
Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade...
April 21, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28429066/atmospheric-pressure-maldi-for-the-noninvasive-characterization-of-carbonaceous-ink-from-renaissance-documents
#13
Giuseppe Grasso, Marzia Calcagno, Alessandro Rapisarda, Roberta D'Agata, Giuseppe Spoto
The analytical methods that are usually applied to determine the compositions of inks from ancient manuscripts usually focus on inorganic components, as in the case of iron gall ink. In this work, we describe the use of atmospheric pressure/matrix-assisted laser desorption ionization-mass spectrometry (AP/MALDI-MS) as a spatially resolved analytical technique for the study of the organic carbonaceous components of inks used in handwritten parts of ancient books for the first time. Large polycyclic aromatic hydrocarbons (L-PAH) were identified in situ in the ink of XVII century handwritten documents...
April 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28426182/usefulness-and-accuracy-of-maldi-tof-mass-spectrometry-as-a-supplementary-tool-to-identify-mosquito-vector-species-and-to-invest-in-development-of-international-database
#14
F N Raharimalala, T M Andrianinarivomanana, A Rakotondrasoa, J M Collard, S Boyer
Arthropod-borne diseases are important causes of morbidity and mortality. The identification of vector species relies mainly on morphological features and/or molecular biology tools. The first method requires specific technical skills and may result in misidentifications, and the second method is time-consuming and expensive. The aim of the present study is to assess the usefulness and accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a supplementary tool with which to identify mosquito vector species and to invest in the creation of an international database...
April 20, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28424252/comparison-of-saramis-4-12-and-ivd-3-0-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-identification-of-mycobacteria-from-solid-and-liquid-culture-media
#15
Caroline Leyer, Guillaume Gregorowicz, Faiza Mougari, Laurent Raskine, Emmanuelle Cambau, Dominique de Briel
During the last decade, many publications studied the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for mycobacterial identification. Diverse and contradictory results indicated that optimal level for routine testing has not been reached yet. This work aimed to assess Vitek MS through two distinct versions, Saramis v4.12 RUO and the IVD v3.0, under conditions close to routine laboratory practice. Overall, 111 mycobacterial isolates were submitted to protein extraction and same spectra were matched against both databases...
April 19, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28421404/characterization-of-zno-nanoparticles-using-superconducting-tunnel-junction-cryodetection-mass-spectrometry
#16
Logan D Plath, Zongyu Wang, Jiajun Yan, Krzysztof Matyjaszewski, Mark E Bier
Zinc oxide (ZnO) nanoparticles coated with either n-octylamine (OA) or α-amino poly(styrene-co-acrylonitrile) (PSAN) ligands (L) have been analyzed using laser desorption/ionization and matrix assisted laser desorption/ionization (MALDI) time-of-flight (TOF) superconducting tunnel junction (STJ) cryodetection mass spectrometry. STJ cryodetection has the advantage of high m/z detection and allows for the determination of average molecular weights and dispersities for 500-600 kDa ZnO-L nanoparticles. The ability to detect the relative energies deposited into the STJs has allowed for investigation of ZnO-L metastable fragmentation...
April 18, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28418253/species-level-discrimination-of-psychrotrophic-pathogenic-and-spoilage-gram-negative-raw-milk-isolates-using-a-combined-maldi-tof-ms-proteomics-bioinformatics-based-approach
#17
Nuwan R Vithanage, Thomas Richard Yeager, Jeevana Bhongir, Snehal R Jadhav, Chaminda Senaka Ranadheera, Enzo A Palombo, Nivedita Datta
Identification of psychrotrophic pathogenic and spoilage Gram-negative bacteria using rapid and reliable techniques is important in commercial milk processing, as these bacteria can produce heat-resistant proteases and act as post-processing contaminants in pasteurised milk. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is a proven technology for identification of bacteria in food, however may require optimisation for identification of pathogenic and spoilage bacteria in milk and dairy products...
April 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28414107/antimicrobial-susceptibility-of-bacteroides-fragilis-group-organisms-in-hong-kong-by-the-tentative-eucast-disc-diffusion-method
#18
Pak-Leung Ho, Chong-Yee Yau, Lok-Yan Ho, Eileen Ling-Yi Lai, Melissa Chun-Jiao Liu, Cindy Wing-Sze Tse, Kin-Hung Chow
This study used a recently developed EUCAST disc diffusion method to measure the susceptibility of 741 B. fragilis group isolates to six antibiotics. Isolates nonsusceptible to imipenem and metronidazole by the disc method were further investigated by E-test. Species identification was obtained by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), PCR assays and 16S rRNA sequencing. The most common species were B. fragilis (n = 424, including 81 division II and 343 division I isolates), B...
April 14, 2017: Anaerobe
https://www.readbyqxmd.com/read/28412280/evaluation-of-sample-preparation-protocols-for-spider-venom-profiling-by-maldi-tof-ms
#19
Ondřej Bočánek, Ondrej Šedo, Stano Pekár, Zbyněk Zdráhal
Spider venoms are highly complex mixtures containing biologically active substances with potential for use in biotechnology or pharmacology. Fingerprinting of venoms by Matrix-Assisted Laser Desorption-Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) is a thriving technology, enabling the rapid detection of peptide/protein components that can provide comparative information. In this study, we evaluated the effects of sample preparation procedures on MALDI-TOF mass spectral quality to establish a protocol providing the most reliable analytical outputs...
April 12, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28411106/mass-spectrometry-imaging-shows-major-derangements-in-neurogranin-and-in-purine-metabolism-in-the-triple-knockout-3%C3%A3-tg-alzheimer-mouse-model
#20
Clara Esteve, Emrys A Jones, Douglas B Kell, Hervé Boutin, Liam A McDonnell
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) can simultaneously measure hundreds of biomolecules directly from tissue. Using different sample preparation strategies, proteins and metabolites have been profiled to study the molecular changes in a 3×Tg mouse model of Alzheimer's disease. In comparison with wild-type (WT) control mice MALDI-MSI revealed Alzheimer's disease-specific protein profiles, highlighting dramatic reductions of a protein with m/z 7560, which was assigned to neurogranin and validated by immunohistochemistry...
April 11, 2017: Biochimica et Biophysica Acta
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