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https://www.readbyqxmd.com/read/28222449/characterization-of-cutaneous-plasmacytosis-at-different-disease-stages
#1
Pa-Fan Hsiao, Yu-Hung Wu
BACKGROUND/AIMS: Cutaneous plasmacytosis is rare and still not well understood. A retrospective study was made of 9 Chinese patients with 1- to 15-year histories of biopsy-proven cutaneous plasmacytosis diagnosed between 2003 and 2015. METHODS: Patient records and archival photographs helped establish the pattern and duration of skin lesions, and skin biopsy specimens provided additional data. RESULTS: The mean age at diagnosis was 46.4 years...
February 22, 2017: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/28220870/long-term-use-of-ceftriaxone-sodium-induced-changes-in-gut-microbiota-and-immune-system
#2
Yanjie Guo, Xuefei Yang, Yane Qi, Shu Wen, Yinhui Liu, Shaoying Tang, Rongsheng Huang, Li Tang
Antibiotic administration, while facilitating clearance of targeted infections, also perturbs commensal microbial communities. Previous studies have all focused on the effects of short term use of antibiotics. Here, we focus on the effects of long term use of antibiotic on gut microbiota and immunity. BALB/c mice received saline or different doses of ceftriaxone sodium (100, 200 and 400 mg/mL) via daily gavage for 150 days. Alterations of fecal microbiota, small intestine histopathology, body weight, spleen index, serum IgG, mucus SIgA, IFN-γ/IL-4 ratio, CD4/CD8 ratio and CD4(+)CD25(+) cells were evaluated...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219528/mycobacterium-tuberculosis-rv1987-induces-th2-immune-responses-and-enhances-mycobacterium-smegmatis-survival-in-mice
#3
Shanshan Sha, Xiaoxia Shi, Guoying Deng, Lina Chen, Yi Xin, Yufang Ma
Mycobacterium tuberculosis can interfere with host immune response and escape clearance through its specific antigens. M. tuberculosis Rv1987 encoded by region of difference (RD)-2 gene is a secretory protein with immunogenic potency. Here, we investigated the impact of Rv1987 on host cytokine responses and T cell polarization in mouse aerosol model. A recombinant M. smegmatis mc(2)155 strain that overexpressed Rv1987 protein (named MS1987) was constructed and used to infect C57BL/6 mice. The mc(2)155 harbored the empty vector (named MSVec) was as a control...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28219072/human-allergen-specific-igg-subclass-antibodies-measured-using-immunocap-technology
#4
Robert Movérare, Karin Blume, Peter Lind, René Crevel, Åsa Marknell DeWitt, Stella Cochrane
BACKGROUND: Knowledge of human IgG subclass antibody responses to various allergens has been hampered by a lack of reliable standardized assays. The aim here was to develop quantitative immunoassays for human IgG1, IgG2, and IgG3 antibodies using ImmunoCAP® technology and to evaluate their application. METHODS: Enzyme conjugates with isotype-specific monoclonal antibodies and calibrators composed of purified myeloma paraproteins were developed for each assay and used together with other standardized assay reagents for the Phadia® 100 instrument...
February 21, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28218991/follow-up-of-surgically-treated-patients-with-cystic-echinococcosis-can-novel-recombinant-antigens-compete-with-imaging-analysis-of-a-patient-cohort
#5
Marija Stojkovic, Hans-Micha Adt, Kerstin Rosenberger, Ghalia Boubaker, Ana Hernandez-Gonzalez, Thomas Junghanss, Marcel Zwahlen, Mar Siles Lucas
OBJECTIVE: To compare the performance of two novel recombinant antigens (EgP29, 2B2t) with imaging in a well-defined cohort of surgically treated cystic echinococcosis (CE) patients to determine whether serology reflects surgical cure as defined by imaging. METHODS: From a cohort of 223 CE-confirmed patients of a national clinical center for echinococcosis, 36 surgically treated patients were eligible for analysis. Sera were tested by enzyme-linked immunosorbent assay (ELISA) for specific IgG and IgG4 antibodies against the EgP29 and 2B2t antigens...
February 20, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28218620/endothelial-antigen-assembly-leads-to-thrombotic-complications-in-heparin-induced-thrombocytopenia
#6
Vincent Hayes, Ian Johnston, Gowthami M Arepally, Steven E McKenzie, Douglas B Cines, Lubica Rauova, Mortimer Poncz
Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies against complexes between human platelet factor 4 (hPF4) and heparin. A better understanding of the events that initiate the prothrombotic state may improve approaches to antithrombotic management. Here, we visualized thrombus formation in an in vivo murine model and an endothelialized microfluidic system that simulate the pathogenesis of HIT. hPF4 released from platelets predominantly bound to peri-injury endothelium and formed HIT antigenic complexes that were dissociated by heparin...
February 20, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28217265/rubella-seromarkers-and-determinants-of-infection-among-tanzanian-children-and-adolescents-in-prevaccination-era-are-we-in-the-right-track
#7
Mariam M Mirambo, Said Aboud, Uwe Groß, Mtebe Majigo, Martha F Mushi, Stephen E Mshana
BACKGROUND: The World health organization advocates assessment of the burden of rubella and congenital rubella syndrome (CRS) by seroepidemiological surveys and surveillance programs in all countries without vaccination programs. Due to scarcity of data in developing countries, this study was conducted to assess the seromakers for natural rubella infection in Tanzania during prevaccination era so as to ascertain the gaps for future research and prevention strategies. METHODS: A cross-sectional study was conducted between September and October 2014...
2017: International Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28216071/antiphospholipid-antibodies-disappearance-in-primary-antiphospholipid-syndrome-thrombosis-recurrence
#8
REVIEW
Gabriela Medina, Eduardo Briones-García, María Pilar Cruz-Domínguez, Oscar I Flórez-Durante, Luis J Jara
OBJECTIVE: To evaluate the clinical outcome after aPL (antiphospholipid antibodies) disappearance in primary APS patients. METHODS: From a cohort of 70 patients with primary APS, we selected patients with positive aPL determinations at onset and ≥2 subsequent negative aPL determinations during the last 5years. To corroborate the immunologic profile, we determined IgG/IgM aCL antibodies, IgG/IgM antiβ2GPl, anti-annexin A5 antibodies and lupus anticoagulant (LA)...
February 13, 2017: Autoimmunity Reviews
https://www.readbyqxmd.com/read/28215759/impact-of-ageing-and-a-synbiotic-on-the-immune-response-to-seasonal-influenza-vaccination-a-randomised-controlled-trial
#9
Sumia Enani, Agnieszka Przemska-Kosicka, Caroline E Childs, Catherine Maidens, Honglin Dong, Lorenza Conterno, Kieran Tuohy, Susan Todd, Margot Gosney, Parveen Yaqoob
BACKGROUND & AIMS: Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects ...
January 28, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28214589/enhanced-expression-and-purification-of-camelid-single-domain-vhh-antibodies-from-classical-inclusion-bodies
#10
Maristella Maggi, Claudia Scotti
Single domain antibodies (sdAbs) are small antigen-binding domains derived from naturally occurring, heavy chain-only immunoglobulins isolated from camelid and sharks. They maintain the same binding capability of full-length IgGs but with improved thermal stability and permeability, which justifies their scientific, medical and industrial interest. Several described recombinant forms of sdAbs have been produced in different hosts and with different strategies. Here we present an optimized method for a time-saving, high yield production and extraction of a poly-histidine-tagged sdAb from Escherichia coli classical inclusion bodies...
February 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28214535/multilevel-ensemble-model-for-prediction-of-iga-and-igg-antibodies
#11
Divya Khanna, Prashant Singh Rana
Identification of antigen for inducing specific class of antibody is prime objective in peptide based vaccine designs, immunodiagnosis, and antibody productions. It's urge to introduce a reliable system with high accuracy and efficiency for prediction. In the present study, a novel multilevel ensemble model is developed for prediction of antibodies IgG and IgA. Epitope length is important in training the model and it is efficient to use variable length of epitopes. In this ensemble approach, seven different machine learning models are combined to predict variable length of epitopes (4 to 50)...
February 15, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28212681/toxoplasma-gondii-survey-in-cats-from-two-environments-of-the-city-of-rio-de-janeiro-brazil-by-modified-agglutination-test-on-sera-and-filter-paper
#12
Paula F Bolais, Philippe Vignoles, Pamela F Pereira, Rafael Keim, Abdelkrim Aroussi, Khadja Ismail, Marie-Laure Dardé, Maria Regina Amendoeira, Aurélien Mercier
BACKGROUND: Toxoplasma gondii is a protozoan with a worldwide distribution, in warm-blood animals, including humans. Local conditions and environmental disturbances may influence transmission dynamics of a zoonotic agent. This study evaluates the epidemiology of T. gondii based on toxoplasmosis prevalence in two populations of cats living in distinct urbanization conditions in Rio de Janeiro, Brazil. METHODS: Among 372 domestic cats sampled, 265 were from a public shelter located downtown Rio and 107 from a relatively preserved wild environment in a residential area...
February 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28212662/marked-central-nervous-system-pathology-in-cd59-knockout-rats-following-passive-transfer-of-neuromyelitis-optica-immunoglobulin-g
#13
Xiaoming Yao, Alan S Verkman
Neuromyelitis optica spectrum disorders (herein called NMO) is an inflammatory demyelinating disease of the central nervous system in which pathogenesis involves complement-dependent cytotoxicity (CDC) produced by immunoglobulin G autoantibodies targeting aquaporin-4 (AQP4-IgG) on astrocytes. We reported evidence previously, using CD59(-/-) mice, that the membrane-associated complement inhibitor CD59 modulates CDC in NMO (Zhang and Verkman, J. Autoimmun. 53:67-77, 2014). Motivated by the observation that rats, unlike mice, have human-like complement activity, here we generated CD59(-/-) rats to investigate the role of CD59 in NMO and to create NMO pathology by passive transfer of AQP4-IgG under conditions in which minimal pathology is produced in normal rats...
February 17, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28211077/severe-bullous-pemphigoid-associated-with-pembrolizumab-therapy-for-metastatic-melanoma-with-complete-regression
#14
O Rofe, G Bar-Sela, Z Keidar, T Sezin, C D Sadik, R Bergman
Bullous pemphigoid (BP) is considered to be a humorally mediated autoimmune disease, but autoreactive T-cells and T-regulatory cells (Tregs) have also been implicated in this disease. Tregs and the programmed death-1 (PD-1) : programmed death ligand (PD-L) pathway are both critical in terminating immune response, and elimination of either can result in breakdown of tolerance and development of autoimmunity. We report a patient with metastatic malignant melanoma (MM), who underwent pembrolizumab (anti-PD-1) therapy following unsuccessful treatment with ipilimumab [anti-cytotoxic T-lymphocyte-associated protein (CTLA)-4]...
February 16, 2017: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/28210565/label-free-proteome-analysis-of-plasma-from-patients-with-breast-cancer-stage-specific-protein-expression
#15
Marina Duarte Pinto Lobo, Frederico Bruno Mendes Batista Moreno, Gustavo Henrique Martins Ferreira Souza, Sara Maria Moreira Lima Verde, Renato de Azevedo Moreira, Ana Cristina de Oliveira Monteiro-Moreira
Breast cancer is one of the most commonly diagnosed types of cancer among women. Breast cancer mortality rates remain high probably because its diagnosis is hampered by inaccurate detection methods. Since changes in protein expression as well as modifications in protein glycosylation have been frequently reported in cancer development, the aim of this work was to study the differential expression as well as modifications of glycosylation of proteins from plasma of women with breast cancer at different stages of disease (n = 30) compared to healthy women (n = 10)...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28203642/epitopes-within-recombinant-%C3%AE-actinin-protein-is-serodiagnostic-target-for-trichomonas-vaginalis-sexually-transmitted-infections
#16
J F Alderete
To date there is no commercially-available serodiagnostic for women and men infected with Trichomonas vaginalis. Thirteen epitopes of the immunogenic T. vaginalis α-actinin (106.2-kDa) are detected by sera of women patients, and 5 epitopes, a subset of the 13, are detected by sera of men. A truncated recombinant protein called ACT-P2 (63.5-kDa) encoding the 5 epitopes is used for screening by ELISA for antibody in sera of women and men. Because ACT-P2 is poorly expressed in E. coli, we wanted alternative recombinant α-actinin proteins as serodiagnostic targets...
January 2017: Heliyon
https://www.readbyqxmd.com/read/28202789/use-of-multiplex-real-time-pcr-to-diagnose-scrub-typhus
#17
Wiwit Tantibhedhyangkul, Ekkarat Wongsawat, Saowaluk Silpasakorn, Duangdao Waywa, Nuttawut Saenyasiri, Jintapa Suesuay, Wilawan Thipmontree, Yupin Suputtamongkol
Scrub typhus, caused by Orientia tsutsugamushi, is a common cause of acute undifferentiated febrile illness in the Asia--Pacific region. However, its non-specific clinical manifestation often prevents early diagnosis. We propose the use of PCR and serologic tests as diagnostic tools. Here, we developed a multiplex real-time PCR assay using hydrolysis (Taqman) probes targeting O. tsutsugamushi47-kDa, groEL, and human IFNB genes to improve early diagnosis of scrub typhus. The amplification efficiency was higher than 94%, and the lower detection limit was 10 copies per reaction...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28198768/long-term-follow-up-of-humoral-immune-status-in-adult-lung-transplant-recipients
#18
Diana A van Kessel, Thijs W Hoffman, Johanna M Kwakkel-van Erp, Erik-Jan D Oudijk, Pieter Zanen, Ger T Rijkers, Jan C Grutters
BACKGROUND: Lung transplant recipients have an increased risk for infections in the posttransplant period due to immunosuppressive therapy. Protection against infections can be achieved through vaccination, but the optimal vaccination schedule in lung transplant recipients is unknown. Data on long-term immunological follow up and vaccination responses after lung transplantation are scarce. METHODS: Here we present long-term immunological follow up of a cohort of 55 lung transplant recipients...
February 14, 2017: Transplantation
https://www.readbyqxmd.com/read/28198544/dengue-virus-and-malaria-concurrent-infection-among-febrile-subjects-within-ilorin-metropolis-nigeria
#19
Olatunji Matthew Kolawole, Adebimpe Adetola Seriki, Ahmad Adebayo Irekeola, Kizito Eneye Bello, Oluwapelumi Olufemi Adeyemi
Dengue is a mosquito-borne disease of public health importance. It is caused by four serotypes of Dengue virus (i.e. DENV-1, -2, -3 and -4). As a result of practices that are conducive for mosquito breading, its vector is widespread in Nigeria and this could result to possible DENV outbreaks in Nigeria and beyond. This study aimed to assess the recency of DENV infection as well as occurrence of DENV and Malaria co-infections within Ilorin, Nigeria. Blood samples were obtained from 176 febrile subjects and analyzed using Enzyme Linked Immunosorbent Assay (ELISA) for the presence of DENV antibodies...
February 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28194450/assessment-of-the-adjuvant-activity-of-mesoporous-silica-nanoparticles-in-recombinant-mycoplasma-hyopneumoniae-antigen-vaccines
#20
Veridiana Gomes Virginio, Natalia Costantin Bandeira, Fernanda Munhoz Dos Anjos Leal, Marcelo Lancellotti, Arnaldo Zaha, Henrique Bunselmeyer Ferreira
The adjuvant potential of two mesoporous silica nanoparticles (MSNs), SBa-15 and SBa-16, was assessed in combination with a recombinant HSP70 surface polypeptide domain from Mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia (PEP). The recombinant antigen (HSP70212-600), previously shown as immunogenic in formulation with classic adjuvants, was used to immunize BALB/c mice in combination with SBa-15 or SBa-16 MSNs, and the effects obtained with these formulations were compared to those obtained with alum, the adjuvant traditionally used in anti-PEP bacterins...
January 2017: Heliyon
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