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https://www.readbyqxmd.com/read/27911496/gram-scale-chemical-synthesis-of-base-modified-ribonucleoside-5-o-triphosphates
#1
Muthian Shanmugasundaram, Annamalai Senthilvelan, Anilkumar R Kore
This unit delineates a simple, reliable, straight-forward, general, and efficient chemical method for the synthesis of modified nucleoside-5'-O-triphosphates such as 5-methylcytidine-5'-O-triphosphate (5-Me-CTP), pseudouridine-5'-O-triphosphate (pseudo-UTP), and N(1) -methylpseudouridine-5'-O-triphosphate (N(1) -methylpseudo-UTP), starting from the corresponding nucleoside. The reaction utilizes an improved protection-free "one-pot, three-step" Ludwig synthetic strategy that involves the monophosphorylation of the nucleoside with phosphorous oxychloride followed by reaction with tributylammonium pyrophosphate and subsequent hydrolysis of the resulting cyclic intermediate to furnish the corresponding ribonucleoside triphosphate (NTP) in moderate yields...
December 1, 2016: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/27911458/spondylodiscitis-a-rare-complication-following-percutaneous-nephrostomy
#2
Francesco Chiancone, Maurizio Fedelini, Clemente Meccariello, Luigi Pucci, Marco Fabiano, Paolo Fedelini
Spondylodiscitis is an inflammation of the intervertebral disc and the adjacent vertebral bodies. The spondylodiscitis can not only be a complication of medical interventions such as an operation near spinal column but also urogenital and vascular interventions and intravenous catheter use. A 71-year-old man was admitted to our emergency department with fever and severe abdominal pain. Antibiotic therapy had been performed with intravenous administration of 2 g of ceftriaxone and the patient underwent the placement of a percutaneous nephrostomy according to Seldinger technique...
November 28, 2016: Urologia
https://www.readbyqxmd.com/read/27910831/high-z-structures-of-organic-molecules-their-diversity-and-organizing-principles
#3
Carolyn Pratt Brock
A list has been compiled of 284 well determined organic structures having more than four crystallographically independent molecules or formula units (i.e. Z' > 4). Another 22 structures were rejected because the space group or unit cell was probably misassigned; the rate for that type of error is then only 7%. The space-group frequencies are unusual; half the structures are in Sohncke groups, partly because the fraction of enantiopure structures of resolvable enantiomers is higher than for lower Z' structures...
December 1, 2016: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/27910757/free-radical-scavenging-activities-of-cultured-mycelia-of-paecilomyces-hepiali-ascomycetes-extracts-and-structural-characterization-of-bioactive-components-by-nuclear-magnetic-resonance-spectroscopy
#4
Lucia Ungvarska Mal'ucka, Jarmila Harvanova, Martin Pavlik, Martin Rajtar, Lukas Jarosciak
Current research is focused on testing the cultivation of Paecilomyces hepiali mycelia on various plant substrates and producing fungus or mycelial biomass with qualitatively interesting substances. P. hepiali mycelia was cultivated using solid-state fermentation of different substrates. Mycelial biomass was then analyzed, and antioxidant activity was evaluated using the DPPH radical scavenging method for different ethanolic extracts based on a millet substrate (extract 1) or a chickpea substrate (extract 2)...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910696/a-new-setup-for-localized-implantation-and-live-characterization-of-kev-energy-multiply-charged-ions-at-the-nanoscale
#5
S Guillous, C Bourin, B Ban D'Etat, A Benyagoub, A Cassimi, C Feierstein, E Gardés, E Giglio, S Girard, C Grygiel, A Houel, H Lebius, A Méry, I Monnet, J-M Ramillon, J Rangama, F Ropars, E Verzeroli, M Viteau, A Delobbe
An innovative experimental setup, PELIICAEN, allowing the modification of materials and the study of the effects induced by multiply charged ion beams at the nanoscale is presented. This ultra-high vacuum (below 5 × 10(-10) mbar) apparatus is equipped with a focused ion beam column using multiply charged ions and a scanning electron microscope developed by Orsay Physics, as well as a scanning probe microscope. The dual beam approach coupled to the scanning probe microscope achieves nanometer scale in situ topological analysis of the surface modifications induced by the ion beams...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910671/a-high-speed-compact-microwave-interferometer-for-density-fluctuation-measurements-in-sino-united-spherical-tokamak
#6
H Zhong, Y Tan, Y Q Liu, H Q Xie, Z Gao
A single-channel 3 mm interferometer has been developed for plasma density diagnostics in the Sino-UNIted Spherical Tokamak (SUNIST). The extremely compact microwave interferometer utilizes one corrugated feed horn antenna for both emitting and receiving the microwave. The beam path lies on the equatorial plane so the system would not suffer from beam path deflection problems due to the symmetry of the cross section. A focusing lens group and an oblique vacuum window are carefully designed to boost the signal to noise ratio, which allows this system to show good performance even with the small-diameter central column itself as a reflector, without a concave mirror...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910312/a-composite-reactor-with-wetted-wall-column-for-mineral-carbonation-study-in-three-phase-systems
#7
Chen Zhu, Xizhi Yao, Liang Zhao, H Henry Teng
Despite the availability of various reactors designed to study gas-liquid reactions, no appropriate devices are available to accurately investigate triple-phased mineral carbonation reactions involving CO2 gas, aqueous solutions (containing divalent cations), and carbonate minerals. This report presents a composite reactor that combines a modified conventional wetted-wall column, a pH control module, and an attachment to monitor precipitation reactions. Our test and calibration experiments show that the absorption column behaved largely in agreement with theoretical predictions and previous observations...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910224/separating-and-identifying-the-four-stereoisomers-of-methyl-jasmonate-by-rp-hplc-and-using-cyclodextrins-in-a-novel-way
#8
Adrián Matencio, Mario J Bermejo-Gimeno, Francisco García-Carmona, José Manuel López-Nicolás
INTRODUCTION: Several authors have reported on the different bioactivities of methyl jasmonate (MeJA) stereoisomers. However, no simple, precise and cheap method for separating and identifying them using reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. OBJECTIVE: (1) To create a simple, precise and cheap method for separating and identifying the four stereoisomers present in commercial racemic mixtures of MeJA and (2) to identify the four stereoisomers using molecular docking techniques and coinjection...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910172/imaging-diagnosis-imaging-and-histopathologic-characteristics-of-a-vertebral-hamartoma-in-a-cat
#9
Frances E Taylor-Brown, Christopher R Lamb, Henny Martineau, Clare Muir, Elsa Beltran
A 9-month-old domestic shorthair cat had progressive ambulatory paraparesis, proprioceptive ataxia, and thoracolumbar hyperesthesia. An extradural mass affecting the left pedicle and lamina of the second lumbar vertebra (L2) causing marked spinal cord impingement was identified in magnetic resonance (MR) images. The mass was predominantly calcified in computed tomographic (CT) images. A hemilaminectomy was performed to resect the mass. Clinical signs were greatly improved at 12-month follow-up. The histopathologic diagnosis was vascular hamartoma...
December 1, 2016: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/27910158/hptlc-bioautography-guided-isolation-of-%C3%AE-glucosidase-inhibiting-compounds-from-justicia-secunda-vahl-acanthaceae
#10
Barbara A Theiler, Stefanie Istvanits, Martin Zehl, Laurence Marcourt, Ernst Urban, Lugardo O Espinoza Caisa, Sabine Glasl
INTRODUCTION: α-Glucosidase inhibitors form an essential basis for the development of novel drugs in diabetes type 2 treatment. Searching for α-glucosidase inhibitors in plants, TLC bioautographic assays have been established and improved within the last years. In traditional medicine, extracts from the leaves of Justicia secunda Vahl are used to treat diabetes mellitus symptoms. OBJECTIVE: To screen for α-glucosidase inhibitors in J. secunda via HPTLC bioautography...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910132/a-simple-method-for-preparation-of-pure-68-ga-acetate-precursor-for-formulation-of-radiopharmaceuticals-physicochemical-characteristics-of-the-68-ga-eluate-of-the-sno2-based-68-ge-68-ga-column-generator
#11
Sankha Chattopadhyay, Md Neyar Alam, Madhu Smita, Umesh Kumar, Sujata Saha Das, Luna Barua
Gallium-68 radioisotope is an excellent source in clinical positron emission tomography application due to its ease of availability from germanium-68 ((68) Ge)/gallium-68 ((68) Ga) generator having a shelf life of 1 year. In this paper, a modified method for purification of the primary eluate of (68) Ge-(68) Ga generator by using a small cation exchange resin (Dowex-50) column has been described. The breakthrough of (68) Ge before and after purification of (68) Ga eluate was 0.014% and 0.00027%, respectively...
December 1, 2016: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/27909755/leachate-properties-and-cadmium-migration-through-freeze-thaw-treated-soil-columns
#12
Meng Xu, Yue Zheng, Weiwei Chen, Na Mao, Ping Guo
Soil column leaching experiments were conducted to study the effects of multiple freeze-thaw cycles on the vertical migration of cadmium (Cd). Three Cd-spiked leaching solutions of different properties were derived from snowmelt, sludge, and straw, designated as B, W and J, respectively. The leaching solutions varied in dissolved organic matter (DOM) concentrations in the order of J > W > B. Changes in leachate properties and Cd concentration were observed. The results showed that pH values of all the leachate solutions through freeze-thaw treated soil columns were higher than those of leachates through unfrozen soils...
December 1, 2016: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/27908928/development-and-validation-of-a-normal-phase-chiral-hplc-method-for-analysis-of-afoxolaner-using-a-chiralpak%C3%A2-ad-3-column
#13
Jinyou Zhuang, Satish Kumar, Abu Rustum
Afoxolaner is a new antiparasitic molecule from the isoxazoline family that acts on the insect acarine gamma-aminobutyric acid and glutamate receptors. Isoxazoline family of compounds has been employed as active pharmaceutical ingredient in drug products prescribed for control of fleas and ticks in dogs. Afoxolaner with a chiral center at isoxazoline ring exists as a racemic mixture. A normal phase chiral high performance liquid chromatography analytical method has been developed to verify that afoxolaner is a racemic mixture as demonstrated by specific rotation, as well as to determine enantiomeric purity of single enantiomer samples...
November 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27908498/hydrophilic-interaction-and-reversed-phase-mixed-mode-liquid-chromatography-coupled-to-high-resolution-tandem-mass-spectrometry-for-polar-lipids-analysis
#14
Sara Granafei, Pietro Azzone, Vito Alessandro Spinelli, Ilario Losito, Francesco Palmisano, Tommaso R I Cataldi
A hydrophilic interaction liquid chromatography (HILIC) fused-core column (150×2.1mm ID, 2.7μm particle size) and a short reversed-phase liquid chromatography (RPLC) column (20mm×2.1mm ID, 1.9μm) were serially coupled to perform mixed-mode chromatography (MMC) on complex mixtures of phospholipids (PL). Mobile phase composition and gradient elution program were, preliminarily, optimized using a mixture of phosphatidylcholines (PC), phosphatidylethanolamines (PE), their corresponding lyso-forms (LPC and LPE), and sphingomyelins (SM)...
November 25, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27908409/evaluation-of-sulfur-isotopic-enrichment-of-urine-metabolites-for-the-differentiation-of-healthy-and-prostate-cancer-mice-after-the-administration-of-34-s-labelled-yeast
#15
Oscar Galilea San Blas, Fernando Moreno Sanz, Pilar Herrero Espílez, Rosa María Sainz Menéndez, Juan Carlos Mayo Barallo, Juan Manuel Marchante-Gayón, José Ignacio García Alonso
Sulfur isotopic enrichment of urine metabolites in healthy and prostate cancer mice using (34)S enriched yeast and High Performance Liquid Chromatography coupled to Multicollector Inductively Coupled Plasma Mass Spectrometry (HPLC-MC-ICP-MS) has been evaluated. A 30 weeks experiment (since the eleventh to the fortieth week of life) was carried out collecting the urine of three healthy mice and three transgenic mice with prostate cancer during 24h after a single oral administration of a (34)S enriched yeast slurry...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27908170/high-resolution-digital-autoradiographic-and-dosimetric-analysis-of-heterogeneous-radioactivity-distribution-in-xenografted-prostate-tumors
#16
Oskar V Timmermand, Jenny Nilsson, Sven-Erik Strand, Jörgen Elgqvist
PURPOSE: The first main aim of this study was to illustrate the absorbed dose rate distribution from (177)Lu in sections of xenografted prostate cancer (PCa) tumors using high resolution digital autoradiography (DAR) and compare it with hypothetical identical radioactivity distributions of (90)Y or 7 MeV alpha-particles. Three dosimetry models based on either dose point kernels or Monte Carlo simulations were used and evaluated. The second and overlapping aim, was to perform DAR imaging and dosimetric analysis of the distribution of radioactivity, and hence the absorbed dose rate, in tumor sections at an early time point after injection during radioimmunotherapy using (177)Lu-h11B6, directed against the human kallikrein 2 antigen...
December 2016: Medical Physics
https://www.readbyqxmd.com/read/27908045/source-depth-discrimination-with-a-vertical-line-array
#17
Ewen Conan, Julien Bonnel, Thierry Chonavel, Barbara Nicolas
Source depth estimation with a vertical line array generally involves mode filtering, then matched-mode processing. Because mode filtering is an ill-posed problem if the water column is not well-sampled, concerns for robustness motivate a simpler approach: source depth discrimination considered as a binary classification problem. It aims to evaluate whether the source is near the surface or submerged. These two hypotheses are formulated in terms of normal modes, using the concept of trapped and free modes. Decision metrics based on classic mode filters are proposed...
November 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27907868/purification-of-high-purity-glycyrrhizin-from-licorice-using-hydrophilic-interaction-solid-phase-extraction-coupled-with-preparative-reversed-phase-liquid-chromatography
#18
Wenyi Yu, Hongli Jin, Aijin Shen, Liang Deng, Jianlian Shi, Xingya Xue, Yadong Guo, Yanfang Liu, Xinmiao Liang
Glycyrrhizin (GA), a major bioactive compound in licorice, has been extensively used throughout the world as a medicine to treat chronic viral hepatitis and allergic dermatitis. In this study, a new method based on hydrophilic interaction solid phase extraction (HILIC-SPE) and preparative reversed-phase liquid chromatography (prep-RPLC) was developed to purify GA with high purity from the complex licorice extract. Via evaluation of retention behavior of GA and flavonoids in different commercially available columns, a hydrophilic column--Click XIon was finally chosen for the purification due to its excellent resolution toward GA and flavonoids under HILIC mode...
November 23, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27907867/adsorption-characteristics-of-adsorbent-resins-and-antioxidant-capacity-for-enrichment-of-phenolics-from-two-phase-olive-waste
#19
Zhihong Wang, Chengzhang Wang, Jiaojiao Yuan, Changwei Zhang
In this study, the adsorption properties of nine resins including polyamide resin (30-60), polyamide resin (60-100) AB-8, S-8, D-101, NKA-9, NKA-II, XDA-1 and XDA-4 for enrichment phenolics of the olive waste were investigated. XDA-1 and NKA-II were chosen for further study due to their outstanding adsorption and desorption capacity. XDA-1 and NKA-II had similar adsorption and desorption behaviors for phenolics of olive waste. The adsorption mechanism could be better explained by pseudo second-order kinetics model and Freundlich isotherm model, and the adsorption processes were spontaneously and exothermic...
November 24, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27907855/lc-ms-ms-assay-for-the-quantitation-of-the-tyrosine-kinase-inhibitor-neratinib-in-human-plasma
#20
Brian F Kiesel, Robert A Parise, Alvin Wong, Kiana Keyvanjah, Samuel Jacobs, Jan H Beumer
Neratinib is an orally available tyrosine kinase inhibitor targeting HER2 (ERBB2) and EGFR (ERBB). It is being clinically evaluated for the treatment of breast and other solid tumors types as a single agent or in combination with other chemotherapies. In support of several phase I/II clinical trials investigating neratinib combinations, we developed and validated a novel LC-MS/MS assay for the quantification of neratinib in 100μL of human plasma with a stable isotopic internal standard. Analytes were extracted from plasma using protein precipitation and evaporation of the resulting supernatant followed by resuspension...
November 22, 2016: Journal of Pharmaceutical and Biomedical Analysis
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