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https://www.readbyqxmd.com/read/28445846/a-sharp-robust-and-quantitative-method-by-liquid-chromatography-tandem-mass-spectrometry-for-the-measurement-of-ead-for-acute-radiation-syndrome-and-its-application
#1
Yiwei Zhang, Jian Li, Zhiyun Meng, Xiaoxia Zhu, Hui Gan, Ruolan Gu, Zhuona Wu, Ying Zheng, Jinbin Wei, Guifang Dou
17-Ethinyl-3,17-dihydroxyandrost-5-ene (EAD) is an agent designed for the treatment of acute radiation syndrome (ARS). Given its vital role played in the prevention and mitigation of ARS, the development of a sharp, sensitive and robust liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor the metabolism of EAD in vivo was crucial. A new method was constructed and validated for the determination of EAD with the internal standard of androst-5-ene-3β,17β-diol (5-AED). The blood samples were precipitated with methanol, centrifuged, from which the supernatant was separated on UPLC with C18 column and eluted in gradient with acetonitrile and Milli-Q water both containing 0...
April 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28445409/quantification-of-a-novel-photosensitizer-of-chlorin-e6-c15-monomethyl-ester-in-beagle-dog-plasma-using-hplc-application-to-pharmacokinetic-studies
#2
Xiuxiu Li, Jun Wen, Jingjing Jiang, Xin Zhao, Tingting Zhou, Guorong Fan
Chlorin e6-C15-monomethyl ester (CMME) is a novel photosensitizer, which is synthetized from the degradation products of silkworm excrement. Preclinical studies on the promising photosensitizer CMME are necessary to determine its therapeutic efficacy and druglikeness. A high-performance liquid chromatography with UV detection (HPLC-UV) method was established for the determination of CMME in beagle dog plasma. The sample preparation involved a protein-precipitation method with acetonitrile after the addition of tanshinone IIA as an internal standard (IS)...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28445001/inhibition-properties-of-some-flavonoids-on-carbonic-anhydrase-i-and-ii-isoenzymes-purified-from-human-erythrocytes
#3
Zübeyir Huyut, Şükrü Beydemir, İlhami Gülçin
Carbonic anhydrases (CAs, E.C.4.2.1.1) play a critical role in many important physiological events and treatment of some diseases. Flavonoids or phenolic compounds have been discovered as novel CAs inhibitors instead of the traditional sulfonamides, with different binding to CAs, pro-drug activities, and new inhibition mechanisms. Here, we investigated the inhibition effects of some flavonoids including malvin, callistephin, oenin, pelargonin, silychristin, and 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol (ID-8) against hCA I and II, which purified from human erythrocytes by affinity column chromatography...
April 26, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28444931/study-of-structure-dependent-chromatographic-behavior-of-glycopeptides-using-reversed-phase-nanolc
#4
Petr Kozlik, Radoslav Goldman, Miloslav Sanda
Analysis of glycosylation is challenging due to micro- and macro-heterogeneity of the protein attachment. A combination of liquid chromatography with tandem mass spectrometry is one of the most powerful tools for glycopeptide analysis. In this work, we show the effect of various monosaccharide units on the retention time of glycopeptides. Retention behavior of several glycoforms of six peptides obtained from tryptic digest of haptoglobin, hemopexin and sex hormone binding globulin was studied on a reversed phase chromatographic column...
April 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28444877/identification-and-measurement-of-staphylococcal-enterotoxin-m-sem-from-staphylococcus-aureus-isolate-associated-with-staphylococcal-food-poisoning
#5
Yanying Zhao, Anni Zhu, Junni Tang, Cheng Tang, Juan Chen
Staphylococcus aureus (S. aureus) produces a wide variety of staphylococcal enterotoxins (SEs, SEA to SEX), which are responsible for staphylococcal food poisoning. This study is aimed to establish a system to detect SEM protein in food matrixes. In the present study, sem gene was characterized in a S. aureus isolate H4 associated with food poisoning. The amino acid sequence of the deduced SEM protein was same as that of previously identified SEM from S. aureus 04-02981. Subsequently, mature SEM protein was expressed in E...
April 26, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28444594/bio-transformation-and-stabilization-of-arsenic-as-in-contaminated-soil-using-arsenic-oxidizing-bacteria-and-fecl3-amendment
#6
Santosh Kumar Karn, Xiangliang Pan, Ian R Jenkinson
A combination of biological and chemical methods was applied in the present study to evaluate the removal of arsenic (As) from contaminated soil. The treatment involved As-oxidizing microbes aimed of transforming the more toxic As (III) to less toxic As (V) in the soil. FeCl3 was added at three different concentrations (1, 2, and 3%) to stabilize the As (V). Leaching of the treated soil was investigated by making a soil column and passing tap water through it to determine solubility. Experimental results indicated that the bacterial activity had a pronounced positive effect on the transformation of As, and decreased the soluble exchangeable fraction from 50 to 0...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444459/some-of-the-earliest-depictions-of-the-human-spine-a-glimpse-into-european-history
#7
EDITORIAL
Peter C Oakes, Christina Kirkpatrick, Jens R Chapman, Rod J Oskouian, R Shane Tubbs
INTRODUCTION: Drawings of the human form have a history almost as old as mankind itself. However, illustrations of the human spine as seen with the vertebral column were not seen until much later. This paper reviews some of the early European depictions of the human vertebral column from the twelfth (e.g., Fünfbilderserie "Bone-Man": 1152 A.D.) and thirteenth (e.g., Ashmole 1292) centuries. Man's understanding of his body has evolved over hundreds of years. CONCLUSIONS: This glimpse into our past and early drawings of the human spine illustrate how this particular anatomical structure was perceived almost a millennium ago and would not be structurally correct renditions until Leonardo da Vinci in the fifteenth century...
April 25, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28444354/easy-assessment-of-levofloxacin-and-minocycline-in-relevant-biomimetic-media-by-hplc-uv-analysis
#8
Ana C Matos, Rosana V Pinto, Ana F Bettencourt
Simple, economic and environmental friendly high-performance liquid chromatography methods for levofloxacin and minocycline quantification in biomimetic media were developed and validate including their stability at body temperature, an often neglected evaluation parameter. Both methods are similar only differing in the wavelength setting, i.e., for levofloxacin and minocycline quantification the UV detection was set at 284 and at 273 nm, respectively. The separation of both antibiotics was achieved using a reversed-phase column and a mobile phase consisting of acetonitrile and water (15:85) with 0...
April 24, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28444185/layer-dependent-short-term-synaptic-plasticity-between-excitatory-neurons-in-the-c2-barrel-column-of-mouse-primary-somatosensory-cortex
#9
Sandrine Lefort, Carl C H Petersen
Neurons process information through spatiotemporal integration of synaptic input. Synaptic transmission between any given pair of neurons is typically a dynamic process with presynaptic action potentials (APs) evoking depressing or facilitating postsynaptic potentials when presynaptic APs occur within hundreds of milliseconds of each other. In order to understand neocortical function, it is therefore important to investigate such short-term synaptic plasticity at synapses between different types of neocortical neurons...
April 21, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28443959/ankle-brachial-index-as-a-predictor-of-mortality-in-hemodialysis-a-5-year-cohort-study
#10
Jair Baptista Miguel, Jorge Paulo Strogoff de Matos, Jocemir Ronaldo Lugon
BACKGROUND: Abnormal ankle-brachial index (ABI) has been found to be a strong predictor of mortality in some hemodialysis populations in studies with relatively short periods of follow-up, lower than 2 years. OBJECTIVE: This study aimed to assess the predictive value of abnormal ABI as a risk factor for death among patients on maintenance hemodialysis after a 5-year follow-up. METHODS: A total of 478 patients on hemodialysis for at least 12 months were included in the study...
March 2017: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28443843/atmospheric-pressure-soft-ionization-for-gas-chromatography-with-dielectric-barrier-discharge-ionization-mass-spectrometry-gc-dbdi-ms
#11
Mario F Mirabelli, Jan-Christoph Wolf, Renato Zenobi
In this study, a gas chromatography (GC) system was interfaced to a high-resolution Orbitrap mass spectrometer by means of an active capillary plasma ionization source, based on dielectric barrier discharge ionization (DBDI). This allowed highly efficient soft ionization of gas-phase, chromatographically resolved compounds at ambient pressure. Several pesticides and illicit drugs were analyzed, and the limits of detections (LODs) were as low as 30 pg mL(-1) for the GC-DBDI-MS coupling (corresponding to 60 fg on-column sensitivity) and 30 fg mL(-1) for SPME-GC-DBDI-MS...
April 26, 2017: Analyst
https://www.readbyqxmd.com/read/28443722/reflections-on-o2-as-a-biosignature-in-exoplanetary-atmospheres
#12
Victoria S Meadows
Oxygenic photosynthesis is Earth's dominant metabolism, having evolved to harvest the largest expected energy source at the surface of most terrestrial habitable zone planets. Using CO2 and H2O-molecules that are expected to be abundant and widespread on habitable terrestrial planets-oxygenic photosynthesis is plausible as a significant planetary process with a global impact. Photosynthetic O2 has long been considered particularly robust as a sign of life on a habitable exoplanet, due to the lack of known "false positives"-geological or photochemical processes that could also produce large quantities of stable O2...
April 26, 2017: Astrobiology
https://www.readbyqxmd.com/read/28443365/enhanced-phosphorus-reduction-in-simulated-eutrophic-water-a-comparative-study-of-submerged-macrophytes-sediment-microbial-fuel-cells-and-their-combination
#13
Peng Xu, Enrong Xiao, Dan Xu, Juan Li, Yi Zhang, Zhigang Dai, Qiaohong Zhou, Zhenbin Wu
The phosphorus content reduction in overlying and sediment pore water was attempted by integrating sediment microbial fuel cells (SMFCs) with the submerged macrophyte Vallisneria spiralis. A comparative study was conducted to treat simulated water rich in phosphate with a control and three treatments: SMFC alone (SMFC), submerged macrophytes alone (macophyte), and combined macrophytes and fuel cells (M-SMFC). All treatments increased sediment Eh and pH of the overlying water, thus promoting phosphorus flux from water column to sediments...
April 26, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28443175/the-versatile-approach-a-novel-single-incision-combined-with-anterior-and-posterior-approaches-for-decompression-and-instrumented-fusion-to-treat-tuberculosis-of-the-thoracic-spine
#14
Sudhir Kumar Srivastava, Rishi Anil Aggarwal, Sunil Krishna Bhosale, Kunal Roy, Pradip Sharad Nemade
STUDY DESIGN: Retrospective case series. PURPOSE: To describe a novel single incision that combines anterior and posterior approaches for decompression and instrumented fusion to treat tuberculosis of the thoracic spine and study the neurological and radiological outcomes. OVERVIEW OF LITERATURE: Tuberculosis of the spine remains a major health issue in many developing countries. The options for treating tuberculosis of the thoracic spine include the anterior, posterior, and combined approaches, each with its advantages and disadvantages...
April 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/28442657/structural-characterization-and-biological-activities-of-a-novel-polysaccharide-from-phyllanthus-emblica
#15
Zihan Zeng, Wenjie Lv, Yongshuai Jing, Zhiyan Chen, Liyan Song, Ting Liu, Rongmin Yu
A novel water-soluble polysaccharide named PEPW80-1, with a molecular mass of 4.7 kDa, was isolated from the pulp tissues of Phyllanthus emblica, and purified by sephadex G-100 column and sephacryl S-300 HR chromatography. The structural features of PEPW80-1 were investigated by a combination of acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, gas chromatography-mass spectrometry, scanning electron microscope, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy...
April 25, 2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28442412/hsccc-separation-and-enantiomeric-distribution-of-key-volatile-constituents-of-piper-claussenianum-miq-c-dc-piperaceae
#16
André M Marques, Catharina E Fingolo, Maria Auxiliadora C Kaplan
High Speed Countercurrent Chromatography (HSCCC) technique was used for the preparative isolation of the major leishmanicidal compounds from the essential oils of Piper claussenianum species in Brazil. The essential oils from inflorescences of P. claussenianum were analyzed by GC-FID and GC-MS. The enantiomeric ratio of the major constituents of the P. claussenianum essential oils were determined using a Rt-DEXsm chiral capillary column by GC-FID analysis. It was found an enantiomeric excess of (+)-(E)-nerolidol in the leaves, and (+)-linalool and (+)-(E)-nerolidol in the inflorescences essential oil...
April 22, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28442173/analysis-of-tar-compounds-and-quantification-of-naphthalene-from-thermal-treatment-of-household-biowaste
#17
S Vakalis, F Patuzzi, K Moustakas, A Sotiropoulos, D Malamis, M Baratieri
Household biowaste represent the organic fraction of municipal solid waste and are an underutilized resource. Although previous studies have performed pyrolysis of organic waste, the vast majority has been on specific presorted feedstock or conventional lignocellulosic streams. Therefore, there is a lack of pyrolysis applications on representative food waste as retrieved from households and this can be attributed primarily to their high water content and their degradability. But via the intermediate step of drying, long-term storage and thermal treatment have become possible...
April 22, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28442132/gas-chromatography-with-diode-array-detection-in-series-with-flame-ionisation-detection
#18
Ronda Gras, Jim Luong, Robert A Shellie
We introduce a gas chromatography detection approach that uses diode array detection operated in series with flame ionisation detection and demonstrate the utility of the detection approach for determination of volatile organic compounds. Diode array detection brings ultraviolet - visible spectroscopy (over a range of 190-640nm) onto the capillary gas chromatography time-scale, where average peak widths of analytes are on the order of 3-5s. The non-destructive nature of the diode array detector affords serially-coupled flame ionisation detection...
April 12, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28442131/multipoint-recognition-of-domoic-acid-from-seawater-by-dummy-template-molecularly-imprinted-solid-phase-extraction-coupled-with-high-performance-liquid-chromatography
#19
Xiuping He, Jianlei Chen, Jiangtao Wang, Liju Tan
Due to the high cost of domoic acid (DA), different carboxylic acid compounds including indole-3-acetic acid (IAA), pyrrole-2-formic acid (PFA), pyridine-2,3-dicarboxylic anhydride (PDA), trimesitinic acid (TA) and citric acid (CA) were investigated as dummy templates for the molecularly imprinted solid-phase extraction (MISPE) for selective isolation and pre-concentration of an amnesic shellfish poison (ASP), DA. The highest binding amount of the polymers towards DA was obtained when CA was used as dummy template owing to its high hydrophilicity...
April 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28442005/implications-of-using-different-water-sources-when-hydrologically-compacting-bioretention-columns
#20
Clayton A Stahnke, Cara J Poor
  Many investigators have conducted research on bioretention systems both in the laboratory and field. There is little consensus on which sources of water are best suited to hydrologically compact bioretention columns. Water with low ionic strength can leach ions from soil media, resulting in a different soil chemistry environment than would be found in typical bioretention applications. Soil columns were hydrologically compacted with three different water sources often used in column studies: deionized water, tap water, and rain water...
May 1, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
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