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In-vitro fertilization

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https://www.readbyqxmd.com/read/28719824/supplementation-of-l-carnitine-during-in%C3%A2-vitro-maturation-improves-embryo-development-from-less-competent-bovine-oocytes
#1
Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, Bartosz Kempisty, Marie Machatkova
The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine, fertilized and cultured to the blastocyst stage. The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development...
July 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28719345/h2o2-induced-mild-stress-in-relation-with-in-vitro-ovine-oocyte-developmental-competence-implications-for-blastocyst-apoptosis-and-related-genes-expression
#2
K Nikdel, M Aminafshar, A Mohammadi-Sangcheshmeh, N EmamJomeh-Kashan, E Seyedjafari
In this study, in vitro maturation was performed in presence of various concentrations (0, 10, 100, or 1000 µM) of H2O2. The intracellular glutathione (GSH) level, fertilization, cleavage, and blastocyst rates, total cell number, and apoptotic cell number and expression of Bax, Bcl-2, and p53 genes in blastocyst-stage embryos were studied. At 10 μM H2O2 concentration, a higher GSH level was detected in comparison to the other groups while oocytes exposed to 1000 μM H2O2 had the lowest GSH level. Treatment of oocytes with 1000 μM H2O2 decreased the rate of two pronuclei formation as compared with other groups...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28718894/sperm-cryopreservation-and-assisted-reproductive-technology-outcome-in-patients-with-spinal-cord-injury
#3
A Reignier, J Lammers, C Splingart, D Redhead, J J Labat, S Mirallié, P Barrière, T Fréour
This study aimed to describe spinal cord injured patients' semen characteristics before and after cryopreservation, and assisted reproductive technology cycles outcome compared to the infertile population. Data about sperm analysis and assisted reproductive technology (ART) cycles outcomes for 78 men with spinal cord injury referred for sperm cryopreservation between 1998 and 2013 were retrospectively analysed and compared with a reference group consisting of every Intra Cytoplasmic Sperm Injection (ICSI) cycle performed in our in vitro fertilization unit over the 2009-2014 period...
July 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28718159/the-role-of-varicocele-in-male-factor-subfertility
#4
REVIEW
Dillon Sedaghatpour, Boback M Berookhim
PURPOSE OF REVIEW: Varicocele may play a significant role in a subset of patients presenting with male factor infertility. Despite its relatively high prevalence amongst subfertile men, there has been controversy over the effectiveness of surgical treatments, patient selection, and when to administer treatment, particularly in the era of assisted reproductive technology. RECENT FINDINGS: In line with earlier finding, recent evidence strongly suggests that varicocelectomy improves pregnancy rates and semen parameters...
September 2017: Current Urology Reports
https://www.readbyqxmd.com/read/28718081/blastocyst-utilization-rates-after-continuous-culture-in-two-commercial-single-step-media-a-prospective-randomized-study-with-sibling-oocytes
#5
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas
PURPOSE: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. METHODS: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28718080/a-comparison-of-live-birth-rates-and-perinatal-outcomes-between-cryopreserved-oocytes-and-cryopreserved-embryos
#6
Jacqueline R Ho, Irene Woo, Kristin Louie, Wael Salem, Sami I Jabara, Kristin A Bendikson, Richard J Paulson, Karine Chung
PURPOSE: Prior studies suggest that pregnancy outcomes after autologous oocyte cryopreservation are similar to fresh in vitro fertilization (IVF) cycles. It is unknown whether there are differences in pregnancy and perinatal outcomes between cryopreserved oocytes and cryopreserved embryos. METHODS: This is a retrospective cohort study comparing pregnancy and perinatal outcomes between oocyte and embryo cryopreservation at a university-based fertility center. We included 42 patients and 68 embryo transfers in patients who underwent embryo transfer after elective oocyte preservation (frozen oocyte-derived embryo transfer (FOET)) from 2005 to 2015...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28715087/association-between-endometrial-subendometrial-vasculature-and-embryo-transfer-outcome-a-meta-analysis-and-subgroup-analysis
#7
Jianing Wang, Fei Xia, Ying Zhou, Xuedong Wei, Yanyan Zhuang, Yingxue Huang
OBJECTIVES: To examine the association between endometrial/subendometrial vasculature and in vitro fertilization-embryo transfer (IVF-ET) and frozen embryo transfer (FET) outcomes. METHODS: A meta-analysis of studies using endometrial/subendometrial 3-dimensional ultrasound and power Doppler angiography was performed to examine the vascularization index (VI), flow index (FI), and vascularization-flow index (VFI) in pregnant and nonpregnant women. Ten articles were analyzed, including 895 pregnant women and 882 nonpregnant women...
July 17, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28710641/a-ligand-binding-assay-to-measure-the-affinity-and-specificity-of-sterol-binding-proteins-in-vitro
#8
Rabih Darwiche, Roger Schneiter
Sterols are major constituents of the plasma membrane of eukaryotic cells and serve as a precursor for several classes of signaling molecules, including steroids and hydroxy sterols. They maintain the functionality and permeability barrier of the plasma membrane through lipid-lipid and lipid-protein interactions. The S. cerevisiae pathogen-related yeast proteins 1, 2, and 3 (Pry) belong to a large protein superfamily known as CAP/SCP/TAPS. Members of this superfamily have been implicated in a wide variety of processes, including immune defense in mammals and plants, pathogen virulence, sperm maturation and fertilization, venom toxicity, and prostate and brain cancer...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28708875/predictive-value-of-serum-progesterone-level-on-%C3%AE-hcg-check-day-in-women-with-previous-repeated-miscarriages-after-in-vitro-fertilization
#9
Yong Jin Kim, Jung Ho Shin, Jun Yong Hur, Hoon Kim, Seung-Yup Ku, Chang Suk Suh
OBJECTIVE: To evaluate the predictive value of the progesterone level at the beta-human chorionic gonadotropin (β-hCG) check day for ongoing pregnancy maintenance in in vitro fertilization (IVF) cycles in women with previous unexplained repeated miscarriages. MATERIALS AND METHODS: One hundred and forty-eight women, with visible gestational sac after IVF, were recruited in this observational study. All subjects had unexplained recurrent miscarriages in more than two previous IVF cycles...
2017: PloS One
https://www.readbyqxmd.com/read/28708512/insulin-during-in%C3%A2-vitro-oocyte-maturation-has-an-impact-on-development-mitochondria-and-cytoskeleton-in-bovine-day-8-blastocysts
#10
Denise Laskowski, Renée Båge, Patrice Humblot, Göran Andersson, Marc-André Sirard, Ylva Sjunnesson
Insulin is a key metabolic hormone that controls energy homeostasis in the body, including playing a specific role in regulating reproductive functions. Conditions associated with hyperinsulinemia can lower developmental rates in bovine in vitro embryo production and are linked to decreased fertility in humans, as in cases of obesity or type 2 diabetes. Embryo quality is important for fertility outcome and it can be assessed by choosing scoring standards for various characteristics, such as developmental stage, quality grade, cell number, mitochondrial pattern or actin cytoskeleton structure...
June 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708509/gdf8-activates-p38-mapk-signaling-during-porcine-oocyte-maturation-in%C3%A2-vitro
#11
Junchul David Yoon, Seon-Ung Hwang, Eunhye Kim, Minghui Jin, Soochong Kim, Sang-Hwan Hyun
Growth Differentiation Factor 8 (GDF8) is a member of the transforming growth factor-β (TGF-β) family and has been identified as a strong physiological regulator. This factor is expressed as a paracrine factor in mural granulosa cells. To investigate the effects of GDF8 on the in vitro maturation (IVM) of porcine oocytes, we assessed the quality of matured oocytes as well as the specific gene transcription and protein activation levels in oocytes and cumulus cells (CCs) after IVM and subsequent embryonic development after in vitro fertilization (IVF) and parthenogenetic activation (PA)...
June 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708489/2-methoxystypandrone-improves-in%C3%A2-vitro-produced-bovine-embryo-quality-through-inhibition-of-ikbkb
#12
Ayman Mesalam, Imran Khan, Kyeong-Lim Lee, Seok-Hwan Song, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
2-Methoxystypandrone (2-MS), a naphthoquinone from Polygonum cuspidatum, has been reported to exhibit antioxidant activities. However, its pharmacological properties have not been fully studied in vitro culture system of oocytes and embryos development. In this study, we examined the effects of 2-MS on bovine blastocyst developmental competency during preimplantation using TUNEL assay, qRT-PCR and immunocytochemistry. Oocytes were treated with 1 μM 2-MS during maturation, followed by in vitro fertilized and the zygotes were cultured...
May 19, 2017: Theriogenology
https://www.readbyqxmd.com/read/28707438/long-term-impact-of-the-endocrine-disruptor-tributyltin-on-male-fertility-following-a-single-acute-exposure
#13
Sumonto Mitra, Ankit Srivastava, Shashi Khandelwal
Declining rate of human fertility is a growing concern, where lifestyle and environmental factors play an important role. We recently demonstrated that tributyltin (TBT), an omnipresent endocrine disruptor, affects testicular cells in vitro. In this study, male Wistar rats were gavaged a single dose of 10, 20, and 30 mg/kg TBT-chloride (TBTC) (to mimic accidental exposure in vivo) and sacrificed on day 3 and day 7, respectively. TBT bioavailability was evaluated by estimating total tin content, and essential metal levels were analyzed along with redox molecules (ROS and GSH/GSSG) to understand the effect on physiological conditions...
July 14, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28706605/embryonic-stem-cell-conditioned-medium-supports-in-vitro-maturation-of-mouse-oocytes
#14
Saber Miraki, Aram Mokarizadeh, Omid Banafshi, Vahideh Assadollahi, Mahdad Abdi, Daem Roshani, Fardin Fathi
BACKGROUND: This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). METHODS: Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized in vitro...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28705424/post-implantation-development-reveals-that-biopsy-procedure-can-segregate-healthy-from-unhealthy-bovine-embryos-and-prevent-miscarriages
#15
Clara Slade Oliveira, Carolina Capobiango Romano Quintão, Celio de Freitas, Agostinho Jorge Dos Reis Camargo, Raquel Varella Serapião, Luiz Sérgio de Almeida Camargo
Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73...
June 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28701212/is-the-oocyte-quality-affected-by-endometriosis-a-review-of-the-literature
#16
REVIEW
Ana Maria Sanchez, Valeria Stella Vanni, Ludovica Bartiromo, Enrico Papaleo, Eran Zilberberg, Massimo Candiani, Raoul Orvieto, Paola Viganò
Endometriosis is an estrogen-dependent chronic inflammatory condition that affects women in their reproductive period causing infertility and pelvic pain. The disease, especially at the ovarian site has been shown to have a detrimental impact on ovarian physiology. Indeed, sonographic and histologic data tend to support the idea that ovarian follicles of endometriosis patients are decreased in number and more atretic. Moreover, the local intrafollicular environment of patients affected is characterized by alterations of the granulosa cell compartment including reduced P450 aromatase expression and increased intracellular reactive oxygen species generation...
July 12, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28698476/acute-supplementation-with-high-dose-vitamin-d3-increases-serum-anti-m%C3%A3-llerian-hormone-in-young-women
#17
Nicola A Dennis, Lisa A Houghton, Michael W Pankhurst, Michelle J Harper, Ian S McLennan
Anti-Müllerian hormone (AMH) is a paracrine regulator of ovarian follicles. Vitamin D (Vit D) regulates AMH production in vitro, but its role as a regulator of ovarian AMH production is contentious. If Vit D influences ovarian AMH production, then an acute rise in Vit D level should lead to an acute rise in circulating AMH levels. This hypothesis was tested with a randomized double-blind design, with 18-25-year-old women recruited from the community. The study was conducted in early spring, when the marker of Vit D level (25-hydroxyvitamin D, 25(OH)D) tends to be at its nadir...
July 8, 2017: Nutrients
https://www.readbyqxmd.com/read/28698074/fertiloscopy-improves-in-vitro-fertilization-for-women-with-repeated-implantation-failure
#18
Ruopeng Zhang, Anli Xu, Qingyi Wang, Lirong Zhang, Li Zhu, Shuhua Zhao, Wei Xiong
OBJECTIVE: To assess the effects of fertiloscopy procedure on the outcomes of subsequent in vitro fertilization cycles in patients with repeated implantation failure (RIF). METHODS: A retrospective study was performed on 50 patients underwent fertiloscopy and 41 controls with RIF. Pelvic and intrauterine pathologies related to infertility, clinical pregnancy rate (CPR) and ongoing pregnancy rate (OPR) of subsequent IVF cycles were assessed. RESULTS: Twenty-six out of 50 patients (52%) had abnormal fertiloscopic findings...
July 8, 2017: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/28697910/duphaston-and-human-menopausal-gonadotropin-protocol-in-normally-ovulatory-women-undergoing-controlled-ovarian-hyperstimulation-during-in%C3%A2-vitro-fertilization-intracytoplasmic-sperm-injection-treatments-in-combination-with-embryo-cryopreservation
#19
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To evaluate endocrine characteristics and clinical outcomes in normal ovulatory patients undergoing controlled ovarian hyperstimulation (COH) with the use of a Duphaston and hMG protocol during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatments in combination with frozen-thawed embryo transfer (FET) compared with the characteristics and outcomes of patients undergoing an Utrogestan and hMG protocol. DESIGN: Prospective controlled study...
July 8, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28697393/same-medicine-different-reasons-comparing-women-s-bodily-experiences-of-producing-eggs-for-pregnancy-or-for-profit
#20
Rene Almeling, Iris L Willey
Women doing in vitro fertilization (IVF) to have a child describe it as painful and emotionally draining. Egg donors undergo the same medical regimen for a different reason - to produce eggs for another woman in exchange for thousands of dollars - and describe it as quick and relatively painless. Medical researchers typically compare bodily responses by variables such as gender, age, and health status. We use the case of "egg production" to propose a new factor that may be an important source of variation in bodily experience: an individual's reason for undergoing the medical intervention in the first place...
June 22, 2017: Social Science & Medicine
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