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https://www.readbyqxmd.com/read/27905280/diagnosis-and-species-discrimination-of-positive-malaria-samples-by-real-time-polymerase-chain-reaction
#1
Esra Atalay Şahar, Muhammet Karakavuk, Hüseyin Can, Şebnem Nergiz, Kadri Gül, Aysu Değirmenci Döşkaya, Yüksel Gürüz, Mert Döşkaya
OBJECTIVE: Malaria is an important tropical disease that is detected in 198 million people and causes 367-755 thousand deaths annually. Recently, the real-time Polymerase Chain Reaction (PCR) technique has enabled quick determination of Plasmodium spp. and species identification in the same assay with a low contamination risk. In the present study, we aimed to use real-time PCR targeting the 18S rRNA gene to diagnose Plasmodium spp. and perform species identification. METHODS: DNA samples of 15 patients with malaria (14 caused by P...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27899111/plasmodium-malariae-in-the-colombian-amazon-region-you-don-t-diagnose-what-you-don-t-suspect
#2
Carlos Hernando Niño, Juan Ricardo Cubides, Paola Andrea Camargo-Ayala, Carlos Arturo Rodríguez-Celis, Teódulo Quiñones, Moisés Tomás Cortés-Castillo, Lizeth Sánchez-Suárez, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
BACKGROUND: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department...
November 29, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27895268/credit-with-health-education-in-benin-a-cluster-randomized-trial-examining-impacts-on-knowledge-and-behavior
#3
Dean Karlan, Bram Thuysbaert, Bobbi Gray
We evaluate whether health education integrated into microcredit lending groups reduces health risks by improving health knowledge and self-reported behaviors among urban and rural borrowers in eastern Benin. In 2007, we randomly assigned 138 villages in the Plateau region of Benin to one of four variations of a group liability credit product, varying lending groups' gender composition and/or inclusion of health education using a 2 × 2 design. Women in villages receiving health education, regardless of gender composition of the groups, showed improved knowledge of malaria and of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS), but not of childhood illness danger signs...
November 28, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27893838/performance-evaluation-of-malaria-microscopists-at-defense-health-facilities-in-addis-ababa-and-its-surrounding-areas-ethiopia
#4
Tigist Yitbarek, Desalegn Nega, Geremew Tasew, Bineyam Taye, Kassu Desta
BACKGROUND: Blood film microscopy is the gold standard approach for malaria diagnosis, and preferred method for routine patient diagnosis in health facilities. However, the inability of laboratory professionals to correctly detect and identify malaria parasites microscopically leads to an inappropriate administration of anti-malarial drugs to the patients and incorrect findings in research areas. This study was carried out to evaluate the performance of laboratory professionals in malaria diagnosis in health facilities under the Defense Health Main Department in Addis Ababa and its surroundings, Ethiopia...
2016: PloS One
https://www.readbyqxmd.com/read/27885872/improvement-of-solubility-dissolution-and-stability-profile-of-artemether-solid-dispersions-and-self-emulsified-solid-dispersions-by-solvent-evaporation-method
#5
Muhammad Tayyab Ansari, Muhammad Sohail Arshad, Altaf Hussain, Zeeshan Ahmad
The purpose of this study was to investigate changes in the water solubility of artemether; a poorly soluble drug used for the treatment of malaria. Different solid dispersions (SD) of artemether were prepared using; artemether and polyethyleneglycol 6000 at ratio 12:88 (Group 1), self-emulsified solid dispersions (SESD) containing artemether, polyethylene glycol 6000, cremophor-A-25, olive oil, hydroxypropylmethylcellulose and transcutol in the ratio 12:75:5:4:2:2, respectively (Group 2). SESD's were also prepared by substituting cremophor-A-25 in Group 2 with poloxamer 188 (noted as Group 3)...
November 25, 2016: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/27884146/demographic-and-clinical-profiles-of-plasmodium-falciparum-and-plasmodium-vivax-patients-at-a-tertiary-care-centre-in-southwestern-india
#6
Laura Chery, Jennifer N Maki, Anjali Mascarenhas, Jayashri T Walke, Pooja Gawas, Anvily Almeida, Mezia Fernandes, Marina Vaz, Rakesh Ramanan, Diksha Shirodkar, Maria Bernabeu, Suresh Kumar Manoharan, Ligia Pereira, Rashmi Dash, Ambika Sharma, Riaz Basha Shaik, Rimi Chakrabarti, Prasad Babar, John White, Devaraja G Mudeppa, Shiva Kumar, Wenyun Zuo, Kristen M Skillman, Usheer Kanjee, Caeul Lim, Kathryn Shaw-Saliba, Ashwani Kumar, Neena Valecha, V N Jindal, Anar Khandeparkar, Pradeep Naik, Sunanda Amonkar, Manoj T Duraisingh, Shripad Tuljapurkar, Joseph D Smith, Nagesh Dubhashi, Roque G W Pinto, Maria Silveria, Edwin Gomes, Pradipsinh K Rathod
BACKGROUND: Malaria remains an important cause of morbidity and mortality in India. Though many comprehensive studies have been carried out in Africa and Southeast Asia to characterize and examine determinants of Plasmodium falciparum and Plasmodium vivax malaria pathogenesis, fewer have been conducted in India. METHODS: A prospective study of malaria-positive individuals was conducted at Goa Medical College and Hospital (GMC) from 2012 to 2015 to identify demographic, diagnostic and clinical indicators associated with P...
November 25, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27882866/the-performance-of-community-health-workers-in-the-management-of-multiple-childhood-infectious-diseases-in-lira-northern-uganda-a-mixed-methods-cross-sectional-study
#7
Phillip Wanduru, Moses Tetui, Doreen Tuhebwe, Michael Ediau, Monica Okuga, Christine Nalwadda, Elizabeth Ekirapa-Kiracho, Peter Waiswa, Elizeus Rutebemberwa
BACKGROUND: Community health workers (CHWs) have the potential to reduce child mortality by improving access to care, especially in remote areas. Uganda has one of the highest child mortality rates globally. Moreover, rural areas bear the highest proportion of this burden. The optimal performance of CHWs is critical. In this study, we assess the performance of CHWs in managing malaria, pneumonia, and diarrhea in the rural district of Lira, in northern Uganda. DESIGNS: A cross-sectional mixed methods study was undertaken to investigate the performance of 393 eligible CHWs in the Lira district of Uganda...
2016: Global Health Action
https://www.readbyqxmd.com/read/27875317/cyp2d6-variability-in-populations-from-venezuela
#8
Nancy Moreno, Carlos Flores-Angulo, Cecilia Villegas, Yuselin Mora
CYP2D6 is an important cytochrome P450 enzyme that plays an important role in the metabolism of about 25% of currently prescribed drugs. The presence of polymorphisms in the CYP2D6 gene may modulate enzyme level and activity, thereby affecting individual responses to pharmacological treatments. The most prevalent diseases in the admixed population from Venezuela are cardiovascular and cancer, whereas viral, bacterial and parasitic diseases, particularly malaria, are prevalent in Amerindian populations; in the treatment of these diseases, several drugs that are metabolized by CYP2D6 are used...
December 1, 2016: Drug Metabolism and Personalized Therapy
https://www.readbyqxmd.com/read/27860006/on-the-ecology-and-range-of-anopheles-beklemishevi-diptera-culicidae-with-reference-to-the-taxonomy-of-an-lewisi
#9
Yuri M Novikov
The ecological features and geographic distribution of Anopheles beklemishevi have not been studied extensively. These studies are important in connection with the validity of the 'Anopheles lewisi' taxon. The materials were collected in Russia and Kazakhstan from 1973 to 2012, and species identity was defined by cytogenetic analysis of polytene chromosomes of larvae and adult females. A total of 7,896 specimens from 34 geographic locations was included in the analysis. It was established that An. beklemishevi is distributed from the east coast of the Baltic Sea to the basin of the Lena River, and from the forest-tundra zone to the Altai and Sayan Mountain systems...
December 2016: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/27846879/plasmodium-malariae-and-plasmodium-ovale-infections-in-the-china-myanmar-border-area
#10
Peipei Li, Zhenjun Zhao, Hua Xing, Wenli Li, Xiaotong Zhu, Yaming Cao, Zhaoqing Yang, Jetsumon Sattabongkot, Guiyun Yan, Qi Fan, Liwang Cui
BACKGROUND: The Greater Mekong Subregion is aiming to achieve regional malaria elimination by 2030. Though a shift in malaria parasite species predominance by Plasmodium vivax has been recently documented, the transmission of the two minor Plasmodium species, Plasmodium malariae and Plasmodium ovale spp., is poorly characterized in the region. This study aims to determine the prevalence of these minor species in the China-Myanmar border area and their genetic diversity. METHODS: Epidemiology study was conducted during passive case detection in hospitals and clinics in Myanmar and four counties in China along the China-Myanmar border...
November 15, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27836016/reduction-in-malaria-prevalence-and-increase-in-malaria-awareness-in-endemic-districts-of-bangladesh
#11
Mohammad Shafiul Alam, Mohammad Moktadir Kabir, Mohammad Sharif Hossain, Shamsun Naher, Nur E Naznin Ferdous, Wasif Ali Khan, Dinesh Mondal, Jahirul Karim, A K M Shamsuzzaman, Be-Nazir Ahmed, Akramul Islam, Rashidul Haque
BACKGROUND: Malaria is endemic in 13 districts of Bangladesh. A baseline malaria prevalence survey across the endemic districts of Bangladesh was conducted in 2007, when the prevalence was reported around 39.7 per 1000 population. After two rounds of Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM)-funded intervention by the National Malaria Control Programme (NMCP) and a BRAC-led NGO consortium, a follow-up survey was conducted across the malaria-endemic districts of Bangladesh to measure the change in prevalence rate and in people's knowledge of malaria...
November 11, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27826886/rarity-of-mixed-species-malaria-with-plasmodium-falciparum-and-plasmodium-malariae-in-travelers-to-saarland-in-germany
#12
Josef Yayan, Kurt Rasche
Malaria is an acute, life-threatening infectious disease that spreads in tropical and subtropical regions. Malaria is mainly brought over to Germany by travelers, so the disease can be overlooked due to its nonspecific symptoms and a lack of experience of attending physicians. The aim of this study was to analyze, retrospectively, epidemiological and clinical data from patients examined for malaria. Patient data were collected from hospital charts at the Department of Internal Medicine, Saarland University Medical Center, Germany, for the period of 2004-2012...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27821127/treatment-seeking-behaviour-and-associated-costs-for-malaria-in-papua-indonesia
#13
Muhammad Karyana, Angela Devine, Enny Kenangalem, Lenny Burdarm, Jeanne Rini Poespoprodjo, Ram Vemuri, Nicholas M Anstey, Emiliana Tjitra, Ric N Price, Shunmay Yeung
BACKGROUND: Malaria remains a significant public health issue in Eastern Indonesia, where multidrug resistant Plasmodium falciparum and Plasmodium vivax are highly prevalent. The objective of this study was to describe treatment-seeking behaviour and household costs prior to a change to a unified treatment policy of dihydroartemisinin-piperaquine in Mimika district, Papua province in 2006. METHODS: In 2005 a randomized cross-sectional household survey was conducted to collect data on demographics, socio-economic status (SES), treatment-seeking, case management, and household costs...
November 8, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27762109/a-comparative-study-of-concurrent-dengue-and-malaria-infection-with-their-monoinfection-in-a-teaching-hospital-in-mumbai
#14
Arundhati Barua, Niharika Gill
OBJECTIVE: This comparative cross sectional study was conducted in a teaching hospital in Mumbai during the monsoon of 2014.A significant number of dengue and malaria co-infection cases, along with dengue, malaria and other infective causes of acute febrile illnesses were noted. The objective of the present study was to understand the interplay of both infections. Since the first such reported case in 2005, studies and data on such cases are scarce, hence this study. AIM: To compare the clinical course, laboratory features, severity and outcome of coinfection with monoinfection of malaria and dengue...
August 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27762108/clinical-and-laboratory-profile-of-hospitalized-malarial-patients-an-agra-based-study
#15
Rajesh Deshwal
OBJECTIVE: The clinical presentations and laboratory profile of malaria has been changing over the years. Therefore this study was undertaken to study the clinical profile and laboratory parameters of malarial patients. METHODS: This prospective observational study was undertaken in military hospital with high prevalence of malaria. A total of 320 patients were studied. All patients tested positive by peripheral blood smear or rapid diagnostic test were included...
August 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27760546/spatial-heterogeneity-can-undermine-the-effectiveness-of-country-wide-test-and-treat-policy-for-malaria-a-case-study-from-burkina-faso
#16
Denis Valle, Justin Millar, Punam Amratia
BACKGROUND: Considerable debate has arisen regarding the appropriateness of the test and treat malaria policy broadly recommended by the World Health Organization. While presumptive treatment has important drawbacks, the effectiveness of the test and treat policy can vary considerably across regions, depending on several factors such as baseline malaria prevalence and rapid diagnostic test (RDT) performance. METHODS: To compare presumptive treatment with test and treat, generalized linear mixed effects models were fitted to data from 6510 children under five years of age from Burkina Faso's 2010 Demographic and Health Survey...
October 19, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27756391/household-clustering-of-asymptomatic-malaria-infections-in-xepon-district-savannakhet-province-lao-pdr
#17
Tiengkham Pongvongsa, Daisuke Nonaka, Moritoshi Iwagami, Masami Nakatsu, Panom Phongmany, Futoshi Nishimoto, Jun Kobayashi, Bouasy Hongvanthon, Paul T Brey, Kazuhiko Moji, Toshihiro Mita, Shigeyuki Kano
BACKGROUND: In the Lao PDR, malaria morbidity and mortality have remarkably decreased over the past decade. However, asymptomatic infections in rural villages contribute to the on-going local transmission. The primary objective of this study was to explore the characteristics of infections in a malaria-endemic district of the Lao PDR. The specific objectives were to investigate the prevalence and species of malaria parasites using molecular methods and to assess individual and household parasite levels and the characteristics associated with malaria infection...
October 18, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27743866/why-do-some-primate-malarias-relapse
#18
Nicholas J White
Relapse may have evolved in malaria as a mechanism to avoid suppression by more virulent species in mixed infections, thereby increasing transmission opportunities. Later evolution of long latency in Plasmodium vivax was a necessary adaptation as early hominins moved to colder areas with shorter mosquito breeding seasons. Genetic diversity was maintained through heterologous hypnozoite activation.
October 12, 2016: Trends in Parasitology
https://www.readbyqxmd.com/read/27733285/global-regional-national-and-selected-subnational-levels-of-stillbirths-neonatal-infant-and-under-5-mortality-1980-2015-a-systematic-analysis-for-the-global-burden-of-disease-study-2015
#19
COMMENT
(no author information available yet)
BACKGROUND: Established in 2000, Millennium Development Goal 4 (MDG4) catalysed extraordinary political, financial, and social commitments to reduce under-5 mortality by two-thirds between 1990 and 2015. At the country level, the pace of progress in improving child survival has varied markedly, highlighting a crucial need to further examine potential drivers of accelerated or slowed decreases in child mortality. The Global Burden of Disease 2015 Study (GBD 2015) provides an analytical framework to comprehensively assess these trends for under-5 mortality, age-specific and cause-specific mortality among children under 5 years, and stillbirths by geography over time...
October 8, 2016: Lancet
https://www.readbyqxmd.com/read/27682611/molecular-evidence-of-high-rates-of-asymptomatic-p-vivax-infection-and-very-low-p-falciparum-malaria-in-botswana
#20
Thato Motshoge, Grace K Ababio, Larysa Aleksenko, John Read, Elias Peloewetse, Mazhani Loeto, Tjantilili Mosweunyane, Kentse Moakofhi, Davies S Ntebele, Simon Chihanga, Mpho Motlaleng, Anderson Chinorumba, Moses Vurayai, Jeffrey M Pernica, Giacomo M Paganotti, Isaac K Quaye
BACKGROUND: Botswana is one of eight SADC countries targeting malaria elimination by 2018. Through spirited upscaling of control activities and passive surveillance, significant reductions in case incidence of Plasmodium falciparum (0.96 - 0.01) was achieved between 2008 and 2012. As part of the elimination campaign, active detection of asymptomatic Plasmodium species by a highly sensitive method was deemed necessary. This study was carried out to determine asymptomatic Plasmodium species carriage by nested PCR in the country, in 2012...
September 29, 2016: BMC Infectious Diseases
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