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https://www.readbyqxmd.com/read/29920456/expression-and-properties-of-the-mitochondrial-and-cytosolic-forms-of-fumarase-in-sunflower-cotyledons
#1
Alexander T Eprintsev, Dmitry N Fedorin, Oksana V Sazonova, Abir U Igamberdiev
Fumarase (EC 4.2.1.2) is encoded in sunflower (Helianthus annuus L.) by two genes (FUM1 and FUM2) expressing correspondingly the mitochondrial and the cytosolic form. Both forms have been purified from sunflower cotyledons and characterized. Three quarters of fumarase activity is located in the mitochondrial and one quarter in the cytosolic fraction. The cytosolic form has lower pH optimum than the mitochondrial form, it possesses higher affinity to malate, activated by Mn2+ and less efficiently by Mg2+ while the mitochondrial form is activated only by Mg2+ ...
June 12, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29892132/prediction-of-fatty-acid-composition-of-sunflower-seeds-by-near-infrared-reflectance-spectroscopy
#2
Murat Reis Akkaya
This study was performed in order to evaluate efficiency of near-infrared reflectance spectroscopy (NIRS) for the determination of fatty acid composition ratio of sunflower seeds and to compare performance of calibration methods. Calibration equations were developed using modified partial least squares (MPLS) and partial least squares (PLS) regression methods. Ninety-three sunflower seed varieties were from test field of East Mediterranean Agricultural Research Institute. In order to determine the reference fatty acid values needed to construct calibration in NIRS analysis, sunflower seed samples were analyzed by gas chromatography method...
June 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29892116/rapid-detection-of-adulterated-peony-seed-oil-by-electronic-nose
#3
Xiaobao Wei, Xingfeng Shao, Yingying Wei, Lingzhi Cheong, Leiqing Pan, Kang Tu
Peony seed oil has recently been introduced as a high-quality food oil. Because the high price of peony seed oil may tempt unscrupulous merchants to dilute it with cheaper substitutes, a rapid detection method for likely adulterants is required. In this study, the fatty acid composition of peony seed oil and four less expensive edible oils (soybean oil, corn oil, sunflower oil, and rapeseed oil) were measured by gas chromatography mass spectrometry. Peony oil adulterated by other edible oils was assessed using iodine values to estimate the extent of adulteration...
June 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29891718/genetics-of-alternative-splicing-evolution-during-sunflower-domestication
#4
Chris C R Smith, Silas Tittes, J Paul Mendieta, Erin Collier-Zans, Heather C Rowe, Loren H Rieseberg, Nolan C Kane
Alternative splicing enables organisms to produce the diversity of proteins necessary for multicellular life by using relatively few protein-coding genes. Although differences in splicing have been identified among divergent taxa, the shorter-term evolution of splicing is understudied. The origins of novel splice forms, and the contributions of alternative splicing to major evolutionary transitions, are largely unknown. This study used transcriptomes of wild and domesticated sunflowers to examine splice differentiation and regulation during domestication...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29873538/phosphorus-mining-efficiency-declines-with-decreasing-soil-p-concentration-and-varies-across-crop-species
#5
Stephanie Schelfhout, An De Schrijver, Kris Verheyen, Robbe De Beelde, Geert Haesaert, Jan Mertens
High soil P concentrations hinder ecological restoration of biological communities typical for nutrient-poor soils. Phosphorus mining, i.e., growing crops with fertilization other than P, might reduce soil P concentrations. However, crop species have different P-uptake rates and can affect subsequent P removal in crop rotation, both of which may also vary with soil P concentration. In a pot experiment with three soil-P-levels (High-P: 125-155 mg POlsen /kg; Mid-P: 51-70 mg POlsen /kg; Low-P: 6-21 mg POlsen /kg), we measured how much P was removed by five crop species (buckwheat, maize, sunflower, flax, and triticale)...
July 29, 2018: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29862215/identification-of-candida-albicans-and-candida-dubliniensis-species-isolated-from-bronchoalveolar-lavage-samples-using-genotypic-and-phenotypic-methods
#6
Sahar Kianipour, Mohammad Emami Ardestani, Parvin Dehghan
Background: Candida dubliniensis is a newly diagnosed species very similar to Candida albicans phenotypically and first discovered in the mouth of people with AIDS in 1995. Among the different phenotypic and genotypic methods, a cost-effective method should be selected which makes it possible to differentiate these similar species. Materials and Methods: Polymerase chain reaction (PCR)-restriction fragment length polymorphism with MspI enzyme and the Duplex-PCR method were done by DNA extraction using boiling...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29807582/molecular-and-biochemical-characterization-of-the-sunflower-helianthus-annuus-l-cytosolic-and-plastidial-enolases-in-relation-to-seed-development
#7
M A Troncoso-Ponce, J Rivoal, S Dorion, R Sánchez, M Venegas-Calerón, A J Moreno-Pérez, S Baud, R Garcés, E Martínez-Force
In the present study, we describe the molecular and biochemical characterization of sunflower (Helianthus annuus L.) enolase (ENO, EC 4.2.1.11) proteins, which catalyze the formation of phosphoenolpyruvate, the penultimate intermediate in the glycolytic pathway. We cloned and characterized three cDNAs encoding different ENO isoforms from developing sunflower seeds. Studies using fluorescently tagged ENOs confirmed the predicted subcellular localization of ENO isoforms: HaENO1 in the plastid while HaENO2 and HaENO3 were found in the cytosol...
July 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29807075/characterization-and-immobilization-of-protease-and-lipase-on-chitin-starch-material-as-a-novel-matrix
#8
Mahmut Özacar, Atheer Awad Mehde, Wesen Adel Mehdi, Zeynep Özacar
Protease and lipase were purified from sunflower seeds by frequent purification steps with molecular weights of 72.90 kDa and 27.50 kDa, respectively. The purified lipase and protease were immobilized on various carriers by different methods of immobilization including physical adsorption, ionic binding and covalent binding. The enzymes prepared by covalent binding on a new support materials were made via the combination of chitin and starch had the highest activates. The immobilization was carried out in a simpler way compared with the other immobilization methods which require various chemicals and complicated procedures which is difficult and expensive...
May 25, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29766221/tracking-emerging-mycotoxins-in-food-development-of-an-lc-ms-ms-method-for-free-and-modified-alternaria-toxins
#9
Hannes Puntscher, Mary-Liis Kütt, Philipp Skrinjar, Hannes Mikula, Joachim Podlech, Johannes Fröhlich, Doris Marko, Benedikt Warth
Mycotoxins produced by Alternaria fungi are ubiquitous food contaminants, but analytical methods for generating comprehensive exposure data are rare. We describe the development of an LC-MS/MS method covering 17 toxins for investigating the natural occurrence of free and modified Alternaria toxins in tomato sauce, sunflower seed oil, and wheat flour. Target analytes included alternariol (AOH), AOH-3-glucoside, AOH-9-glucoside, AOH-3-sulfate, alternariol monomethyl ether (AME), AME-3-glucoside, AME-3-sulfate, altenuene, isoaltenuene, tenuazonic acid (TeA), tentoxin (TEN), altertoxin I and II, alterperylenol, stemphyltoxin III, altenusin, and altenuic acid III...
May 16, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29748980/coordinated-plasticity-maintains-hydraulic-safety-in-sunflower-leaves
#10
Amanda A Cardoso, Timothy J Brodribb, Christopher J Lucani, Fábio M DaMatta, Scott A M McAdam
The air-seeding threshold water potential establishes a hydraulic limit on the ability of woody species to survive in water-limiting environments, but herbs may be more plastic in terms of their ability to adapt to drying conditions. Here we examined the capacity of sunflower (Helianthus annuus L.) leaves to adapt to reduced water availability by modifying the sensitivity of xylem and stomata to soil water deficit. We found that sunflower plants grown under water-limited conditions significantly adjusted leaf osmotic potential, which was linked to a prolongation of stomatal opening as soil dried and a reduced sensitivity of photosynthesis to water-stress induced damage...
May 10, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29703595/go-with-your-gut-digestibility-and-digestive-function-of-two-arid-zone-australian-murids-pseudomys-australis-and-notomys-alexis
#11
Hayley J Stannard, Melissa L Tulk, Melissa J Bortolazzo, Julie M Old
Spinifex hopping-mice (Notomys alexis) and plains mice (Pseudomys australis) are able to successfully occupy arid zones of Australia. We studied the digestive parameters and energy assimilation of captive spinifex hopping-mice and plains mice. The experiment consisted of six diets fed to the animals for periods of 12days per food type. On a dry matter basis, the plains mice consumed between 2.5 and 7.2% and the hopping-mice between 5.8 and 9.3% of their body mass in food per day. The body mass of the spinifex hopping-mice increased significantly on the sunflower seed diet, while body mass did not change significantly for the plains mice on any diet...
June 2018: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/29666513/bioactive-compounds-and-in-vitro-antioxidant-activity-of-some-traditional-and-non-traditional-cold-pressed-edible-oils-from-macedonia
#12
Sanja Kostadinović Veličkovska, Augustin Catalin Moţ, Saša Mitrev, Rubin Gulaboski, Ludger Brühl, Hamed Mirhosseini, Radu Silaghi-Dumitrescu, Bertrand Matthäus
The bioactive compounds and "in vitro" antioxidant activity measured by three antioxidant assays of some traditional and non-traditional cold-pressed edible oils from Macedonia were object of this study. The fatty acid composition showed dominance of monounsaturated oleic acid in "sweet" and "bitter" apricot kernel oils with percentages of 66.7 ± 0.5 and 57.8 ± 0.3%, respectively. The most dominant fatty acid in paprika seed oil was polyunsaturated linoleic acid with abundance of 69...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29606208/integrating-proteomics-and-enzymatic-profiling-to-decipher-seed-metabolism-affected-by-temperature-in-seed-dormancy-and-germination
#13
Qiong Xia, Maharajah Ponnaiah, Gwendal Cueff, Loïc Rajjou, Duyen Prodhomme, Yves Gibon, Christophe Bailly, Françoise Corbineau, Patrice Meimoun, Hayat El-Maarouf-Bouteau
Temperature is an important environmental factor affecting seed dormancy and germination. The mechanism by which temperature induces germination in dormant seeds is however still unclear. Proteomic study has been performed in dormant sunflower seeds during imbibition at permissive and non-permissive temperatures for germination, 20 and 10 °C, respectively. Proteome analysis showed an increase of proteins belonging to metabolism and energy from the first hours of imbibition followed by a decrease of proteins involved in protein metabolism and seed storage in germinating compared to non-germinating seeds...
April 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29603433/supplementation-with-sunflower-seeds-in-beef-cattle-did-not-impact-on-oocyte-and-in-vitro-embryo-production
#14
A L Baltazar, G M de Mattos, B M Ropelli, Smg Firetti, C Castilho, G Pugliesi, Mbc Maldonado, M Binelli, Jof Silva, G C Lupatini, B S Lafuente, Cmb Membrive
Supplementation with compounds rich in linoleic acid, including sunflower seed supplementation, promotes increase in conception rates in cows. We aimed to evaluate whether the sunflower seed (linoleic acid source) supplementation in beef donor females alters the plasma concentrations of cholesterol, triglycerides, HDL and LDL, increases the number and quality of oocytes, increases the cleavage rates and determines an improvement in number and quality of in vitro produced blastocysts. Thus, Nelore females were divided into two groups of 15 animals to receive supplementation with or without sunflower seed for 57 days...
June 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29597178/viability-of-honeybee-colonies-exposed-to-sunflowers-grown-from-seeds-treated-with-the-neonicotinoids-thiamethoxam-and-clothianidin
#15
M Dolores Hernando, Victoria Gámiz, Sergio Gil-Lebrero, Inmaculada Rodríguez, Ana I García-Valcárcel, V Cutillas, Amadeo R Fernández-Alba, José M Flores
In this study, honeybee colonies were monitored in a field study conducted on sunflowers grown from seeds treated with the systemic neonicotinoids thiamethoxam or clothianidin. This field trial was carried out in different representative growing areas in Spain over a beekeeping season. The health and development of the colonies was assessed by measuring factors that have a significant influence on their strength and overwintering ability. The parameters assessed were: colony strength (adult bees), brood development, amount of pollen and honey stores and presence and status of the queen...
July 2018: Chemosphere
https://www.readbyqxmd.com/read/29530255/voltammetric-fingerprinting-of-oils-and-its-combination-with-chemometrics-for-the-detection-of-extra-virgin-olive-oil-adulteration
#16
Fotios Tsopelas, Dimitris Konstantopoulos, Anna Tsantili Kakoulidou
In the present work, two approaches for the voltammetric fingerprinting of oils and their combination with chemometrics were investigated in order to detect the adulteration of extra virgin olive oil with olive pomace oil as well as the most common seed oils, namely sunflower, soybean and corn oil. In particular, cyclic voltammograms of diluted extra virgin olive oils, regular (pure) olive oils (blends of refined olive oils with virgin olive oils), olive pomace oils and seed oils in presence of dichloromethane and 0...
July 26, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29518657/magnesium-and-organic-biostimulant-integrative-application-induces-physiological-and-biochemical-changes-in-sunflower-plants-and-its-harvested-progeny-on-sandy-soil
#17
Hafeez Ur Rehman, Hesham F Alharby, Yahya Alzahrani, Mostafa M Rady
Magnesium (Mg) often leaches down in sandy soils due to high mobility and its foliar application proves to be beneficial. Organic biostimulants also prove to be helpful to affect plant physio-biochemistry and antioxidative defense system. The present study evaluated the beneficial effects of seed soaking in maize grain extract (MGE; 3%) in integration with or without foliar Mg (1 mM) in comparison to control (no treatment) on growth, yield performance, seed oil and fatty acid profile including physiological and biochemical basis of Hysun-336 sunflower hybrid grown on a sandy soil under greenhouse conditions...
May 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29513105/survival-of-salmonella-during-production-of-partially-sprouted-pumpkin-sunflower-and-chia-seeds-dried-for-direct-consumption
#18
Susanne E Keller, Nathan M Anderson, Can Wang, Stephen J Burbick, Ian M Hildebrandt, Lauren J Gonsalves, Quincy J Suehr, Sofia M Santillana Farakos
Ready-to-eat foods based on dried partially sprouted seeds have been associated with foodborne salmonellosis. Whereas research has focused on the potential for Salmonella initially present in or on seeds to grow and survive during fresh sprout production, little is known about the potential for growth and survival of Salmonella associated with seeds that have been partially sprouted and dried. The goal of this study was to determine the growth of Salmonella during soaking for partial germination of pumpkin, sunflower, and chia seeds and subsequent survival during drying and storage...
April 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29508721/anaphylaxis-due-to-roasted-sunflower-seeds-with-tolerance-to-raw-sunflower-seeds
#19
Marina Labella Álvarez, Teresa De Aramburu Mera, Krasimira Baynova, Borja Bartolomé Zavala, Manuel Prados Castaño
No abstract text is available yet for this article.
March 2018: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/29501147/high-throughput-quantification-of-sodium-saccharin-in-foods-by-ambient-flame-ionization-mass-spectrometry
#20
Zhongquan Li, Fang Zhang, Junbo Zhao, Xiaopan Liu, Xiuping Chen, Yue Su, Yinlong Guo
Ambient flame ionization (AFI) coupled with triple quadrupole tandem mass spectrometry was developed for quantitative analysis of sodium saccharin (SAC) in various food samples. Typically, the micro-flame by the combustion of n-butane provided a heating zone for fast desorption and ionization of analytes in milliseconds. Then high ion abundance of analyte could be produced in a short time, which made AFI-MS possess a very high sensitivity for SAC detection and was particularly appropriate for the quantification in multiple reaction monitor (MRM) mode...
May 15, 2018: Talanta
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