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https://www.readbyqxmd.com/read/28638389/recognition-of-orobanche-cumana-below-ground-parasitism-through-physiological-and-hyper-spectral-measurements-in-sunflower-helianthus-annuus-l
#1
Amnon Cochavi, Tal Rapaport, Tania Gendler, Arnon Karnieli, Hanan Eizenberg, Shimon Rachmilevitch, Jhonathan E Ephrath
Broomrape (Orobanche and Phelipanche spp.) parasitism is a severe problem in many crops worldwide, including in the Mediterranean basin. Most of the damage occurs during the sub-soil developmental stage of the parasite, by the time the parasite emerges from the ground, damage to the crop has already been done. One feasible method for sensing early, below-ground parasitism is through physiological measurements, which provide preliminary indications of slight changes in plant vitality and productivity. However, a complete physiological field survey is slow, costly and requires skilled manpower...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28608227/bimodality-of-stable-and-plastic-traits-in-plants
#2
Josef Fisher, Elad Bensal, Dani Zamir
We discovered an unexpected mode of bimodal distribution of stable and plastic traits, which was consistent for homologous traits of 32 varieties of seven species both in well-irrigated fields and dry conditions. We challenged archived genetic mapping data for 36 fruit, seed, flower and yield traits in tomato and found an unexpected bimodal distribution in one measure of trait variability, the mean coefficient of variation, with some traits being consistently more variable than others. To determine the degree of conservation of this distribution among higher plants, we compared 18 homologous phenotypes, including yield and seed production, across different crop species grown in a common 'crop garden' experiment...
June 12, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28587895/a-novel-operant-task-to-assess-social-reward-and-motivation-in-rodents
#3
Johnathan M Borland, Kyle J Frantz, Lauren M Aiani, Kymberly N Grantham, Zhimin Song, H Elliott Albers
BACKGROUND: Social reward plays a critical role in the development of beneficial social relationships, and disorders of the mechanisms controlling social reward are involved in the etiology of many psychiatric diseases. NEW METHOD: We present a novel operant social preference task to quantify social reward in rodents using an apparatus with three chambers separated by one-way vertical-swing doors. The experimental animal is placed in the larger chamber while the two smaller chambers either remain empty or contain a stimulus animal or other potential reward stimulus...
June 3, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28580502/in-vitro-bioavailability-of-calcium-magnesium-iron-zinc-and-copper-from-gluten-free-breads-supplemented-with-natural-additives
#4
J Regula, A Cerba, J Suliburska, A A Tinkov
The aim of this study was to measure the content of calcium, magnesium, iron, zinc, and copper and determine the bioavailability of these ingredients in gluten-free breads fortified with milk and selected seeds. Due to the increasing prevalence of celiac disease and mineral deficiencies, it has become necessary to produce food with higher nutritional values which maintains the appropriate product characteristics. This study was designed for gluten-free breads fortified with milk and seeds such as flax, poppy, sunflower seeds, pumpkin seeds or nuts, and flour with amaranth...
June 3, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28573560/phyto-management-of-cr-contaminated-soils-by-sunflower-hybrids-physiological-and-biochemical-response-and-metal-extractability-under-cr-stress
#5
Mujahid Farid, Shafaqat Ali, Nudrat Aisha Akram, Muhammad Rizwan, Farhat Abbas, Syed Asad Hussain Bukhari, Rashid Saeed
Chromium (Cr) is a biologically non-essential, carcinogenic and toxic heavy metal. The cultivation of Cr-tolerant genotypes seems the most favorable and environment friendly strategy for rehabilitation and remediation of Cr-contaminated soils. To prove this hypothesis and identify the Cr tolerance, the present study was performed to assess the physiological and biochemical response of sunflower genotypes to Cr stress. The seeds of six sunflower hybrids, namely FH-425, FH-600, FH-612, FH-614, FH-619, and FH-620, were grown in spiked soil for 12 weeks under increasing concentrations of Cr (0, 5, 10, and 20 mg kg(-1))...
June 1, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28555756/design-and-chemical-syntheses-of-potent-matriptase-2-inhibitors-based-on-trypsin-inhibitor-sfti-1-isolated-from-sunflower-seeds
#6
Agata Gitlin-Domagalska, Dawid Dębowski, Anna Łęgowska, Marit Stirnberg, Joanna Okońska, Michael Gütschow, Krzysztof Rolka
Matriptase-2 plays a pivotal role in keeping iron concentrations within a narrow physiological range in humans. The opportunity to reduce matriptase-2 proteolytic activity may open a novel possibility to treat iron overload diseases, such as hereditary hemochromatosis and thalassemia. Here, we present 23 new analogues of trypsin inhibitor SFTI-1 designed to inhibit human matriptase-2. Influence of the modifications Gly1Lys, Ile10Arg and Phe12His, as well as the introduction of Narg in P1 or P1 and P4 positions were examined...
May 27, 2017: Biopolymers
https://www.readbyqxmd.com/read/28536266/two-proteins-for-the-price-of-one-structural-studies-of-the-dual-destiny-preproalbumin-with-sunflower-trypsin-inhibitor-1
#7
Bastian Franke, Amy M James, Mehdi Mobli, Michelle L Colgrave, Joshua S Mylne, K Johan Rosengren
Seed storage proteins are both an important source of nutrition for humans and essential for seedling establishment. Interestingly, unusual napin-type 2S seed storage albumin precursors in sunflower contain a sequence that is released as a macrocyclic peptide during post-translational processing. The mechanism by which such peptides emerge from linear precursors proteins has received increased attention, however the structural characterization of intact precursor proteins has been limited. Here we report the 3D NMR structure of the Helianthus annuus PreproAlbumin With Sunflower trypsin inhibitor-1 (PawS1), and provide new insights into the processing of this remarkable dual-destiny protein...
May 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28530614/effect-of-traditional-chinese-cooking-methods-on-fatty-acid-profiles-of-vegetable-oils
#8
Yamin Cui, Pengfei Hao, Bingjie Liu, Xianghong Meng
The effect of four frying processes (vegetable salad, stir frying, pan frying, and deep frying) on fatty acid composition of ten vegetable oils (peanut oil, soybean oil, rapeseed oil, corn oil, sunflower seed oil, rice bran oil, olive oil, sesame oil, linseed oil, and peony seed oil) was investigated using GC-MS. The result showed that trans-fatty acid (TFA) was produced during all processes. Rapeseed oil had the highest TFA content in vegetable salad oil with 2.88% of total fatty acid. The TFA content of sunflower seed oil was 0...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28514707/influence-of-fumigants-on-sunflower-seeds-characteristics-of-fumigant-desorption-and-changes-in-volatile-profiles
#9
Nadine Austel, Jens Schubert, Sabrina Gadau, Harald Jungnickel, Lygia T Budnik, Andreas Luch
Fumigation of transport containers is common practice to protect stored products from pests. Yet little is known about the desorption times and effects of the highly toxic gases used in this process. To shed light on the behavior of fumigants in real food, we treated sunflower seeds (Helianthus annuus L.) with 100ppm phosphine (PH3), methyl bromide (MeBr) or 1,2-dichloroethane (DCE) for 72h. The compound concentrations in the air were then analyzed by thermal desorption/2D gas chromatography coupled to mass spectrometry and flame photometric detection (TD-2D-GC-MS/FPD)...
April 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28433057/low-cost-humic-acid-bonded-silica-as-an-effective-solid-phase-extraction-sorbent-for-convenient-determination-of-aflatoxins-in-edible-oils
#10
Neng-Zhi Zhou, Ping Liu, Xiao-Chuan Su, Yan-Hua Liao, Ning-Sheng Lei, Yong-Hong Liang, Shao-Huan Zhou, Wen-Si Lin, Jie Chen, Yu-Qi Feng, Yang Tang
Aflatoxins (AFs) are highly toxic, mutagenic, carcinogenic, and teratogenic secondary metabolites produced by the toxigenic fungi Aspergillus flavus and Aspergillus parasiticus. AFs tend to contaminate a wide range of foods which is a serious and recurring food safety problem worldwide. Currently, immunoaffinity chromatography (IAC) has become the most conventional sample clean-up method for determining AFs in foodstuffs. However, IAC method is limited in the large-scale food analysis because it requires the use of expensive disposable cartridges and the IA procedure is time-consuming...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28419131/aflatoxin-levels-in-sunflower-seeds-and-cakes-collected-from-micro-and-small-scale-sunflower-oil-processors-in-tanzania
#11
Juma A Mmongoyo, Felicia Wu, John E Linz, Muraleedharan G Nair, Jovin K Mugula, Robert J Tempelman, Gale M Strasburg
Aflatoxin, a mycotoxin found commonly in maize and peanuts worldwide, is associated with liver cancer, acute toxicosis, and growth impairment in humans and animals. In Tanzania, sunflower seeds are a source of snacks, cooking oil, and animal feed. These seeds are a potential source of aflatoxin contamination. However, reports on aflatoxin contamination in sunflower seeds and cakes are scarce. The objective of the current study was to determine total aflatoxin concentrations in sunflower seeds and cakes from small-scale oil processors across Tanzania...
2017: PloS One
https://www.readbyqxmd.com/read/28407930/large-scale-multi-class-herbicides-analysis-in-oilseeds-by-rapid-one-step-quechers-based-extraction-and-cleanup-method-using-liquid-chromatography-tandem-mass-spectrometry
#12
Piotr Kaczyński
A simple, sensitive and rapid method is proposed for simultaneous determination of 120 various classes of herbicides in oilseed samples. A one-step QuEChERS-based approach to extraction and cleanup has been developed. LC-MS/MS was used to evaluate the proposed sample treatment methodologies. The new one-step QuEChERS strategy was compared to other methodologies previously used for elimination of co-extracts from fatty matrices. For validation purposes, recovery studies were carried out at 5, 50 and 500µgkg(-1) levels for rapeseed, sunflower seeds and soybean...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28386909/remote-measurement-of-sunflower-seed-moisture-content-by-the-use-of-microwaves
#13
Vitali L Litvischenko, Igor Ya Nikiforov, Igor V Ershov
BACKGROUND: Modern agriculture demands new methods and equipment that allow operators to conduct the instant control of moisture content over a wide area of agricultural fields with the purpose of providing farmers with the optimal moment of harvesting mature seeds and crops. Here the authors propose a new method and experimentally investigate the possibility to accomplish remote sensing of the moisture content of sunflower seeds using microwave radiation in the millimeter range. RESULTS: An experimental device for measuring the coefficient of reflection of electromagnetic waves from sunflower inflorescences in the frequency range 25...
April 7, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28364646/the-influences-of-cr-tolerant-rhizobacteria-in-phytoremediation-and-attenuation-of-cr-vi-stress-in-agronomic-sunflower-helianthus-annuus-l
#14
Ali Bahadur, Rizwan Ahmad, Aftab Afzal, Huyuan Feng, Vishandas Suthar, Asfa Batool, Aman Khan, Muhammad Mahmood-Ul-Hassan
Chromium contamination of agronomic soil has to turn into a serious global problem. This research was pointed to assess the effects of three Cr-tolerant rhizobacteria (SS1, SS3, and SS6) on sunflower growth and heavy metal uptake under Cr smog i.e. 20, 30 and 40 ppm using K2Cr2O7. Root promotion assay and pot experiment were conducted to investigate and evaluate the effects of Cr tolerance rhizobacteria and Cr accumulation capacity of sunflower. From root promotion assay non-significant variation was observed in the root length between SS1 and SS3 compared with un-inoculated whereas SS6 enhanced the root length in the absence and presence of chromium...
July 2017: Chemosphere
https://www.readbyqxmd.com/read/28357795/the-effectiveness-of-dietary-sunflower-meal-and-exogenous-enzyme-on-growth-digestive-enzymes-carcass-traits-and-blood-chemistry-of-broilers
#15
Mahmoud Alagawany, Adel I Attia, Zenat A Ibrahim, Reda A Mahmoud, Sabry A El-Sayed
High costs of conventional protein feed sources including soybean meal (SBM) generated the need for finding other alternatives. Thus, the present study was designed to evaluate the impact of graded replacements of SBM by sunflower seed meal (SFM) with or without enzyme supplementation on growth performance, digestive enzymes, carcass traits, and blood profile of broiler chickens. A total of 240 unsexed 1-week-old broiler chicks (Hubbard) were randomly divided into eight treatment groups of 30 chicks each in five replicates each of six chicks in a factorial design (4 × 2) arrangement, including four levels of SFM (0, 25, 50, and 75% replacing SBM) and two levels of enzyme (0- or 0...
May 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28357621/improved-campesterol-production-in-engineered-yarrowia-lipolytica-strains
#16
Yu Zhang, Ying Wang, Mingdong Yao, Hong Liu, Xiao Zhou, Wenhai Xiao, Yingjin Yuan
OBJECTIVES: To engineer Yarrowia lipolytica for improving the heterologous production of campesterol (a key precursor to manufacture pharmaceutical steroids). RESULTS: By screening 7-dehydrocholesterol reductase (DHCR7) from diverse species, DHCR7 from Danio rerio was the best candidate for campesterol synthesis. Overexpression of ACL (ATP: citrate lyase) or POX2 (peroxisome acyl-CoA oxidase 2) were key to improving campesterol production. The highest yield of campesterol was 942 mg/l was with the strain overexpressing POX2 in a 5 l bioreactor via high cell density fermentation process with a restricted supply of carbon sourc, sunflower seed oil...
March 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28356106/protective-effects-of-various-ratios-of-dha-epa-supplementation-on-high-fat-diet-induced-liver-damage-in-mice
#17
Tingting Shang, Liang Liu, Jia Zhou, Mingzhen Zhang, Qinling Hu, Min Fang, Yongning Wu, Ping Yao, Zhiyong Gong
BACKGROUND: A sedentary lifestyle and poor diet are risk factors for the progression of non-alcoholic fatty liver disease. However, the pathogenesis of hepatic lipid accumulation is not completely understood. Therefore, the present study explored the effects of dietary supplementation of various ratios of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on a high-fat diet-induced lipid metabolism disorder and the concurrent liver damage. METHODS: Using high-fat diet-fed C57BL/6 J mice as the animal model, diets of various ratios of DHA/EPA (2:1, 1:1, and 1:2) with an n-6/n-3 ratio of 4:1 were prepared using fish and algae oils enriched in DHA and/or EPA and sunflower seed oils to a small extent instead of the high-fat diet...
March 29, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28349541/effect-of-the-type-and-level-of-hydration-of%C3%A2-alcoholic-solvents-on-the-simultaneous-extraction-of-oil-and-chlorogenic-acids-from-sunflower-seed-press-cake
#18
Nayara K Scharlack, Keila K Aracava, Christianne Ec Rodrigues
BACKGROUND: The present study aimed to evaluate the replacement of hexane by alcoholic solvents in oil extraction from sunflower seed press cake. The use of ethanol and isopropanol has important advantages, including low toxicity and good operational safety. Thus, in the present study, solid-liquid extractions were performed in a single stage from 60 to 90 °C and in consecutive extractions in three stages at 90 °C. RESULTS: Solvent hydration negatively affected the extraction of oil but favored the extraction of chlorogenic acids (CAs), especially when ethanol was used...
March 27, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28340727/a-quantitative-approach-for-cd-cu-fe-and-mn-through-laser-ablation-imaging-for-evaluating-the-translocation-and-accumulation-of-metals-in-sunflower-seeds
#19
Gustavo de S Pessôa, Cícero A Lopes Júnior, Katherine C Madrid, Marco A Z Arruda
The uptake and accumulation of Cd in sunflower seeds represents an important pathway for imputing potentially toxic metals into human and animal food. In this way, bioimaging of Cd and micronutrients (Cu, Fe and Mn) in the seeds of sunflower grown in soil contaminated with Cd are performed. For this task, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is used in quantitative approach, considering four groups: precursor, control, Cd-low (50mg) and Cd-high (700mg). For attaining our proposals, ICP-MS (nebulizer and auxiliary flow rates and radiofrequency power) and LA (laser intensity, frequency and spot size) parameters were optimized, and the analytical signal improved to 197%, 217%, 232%, and 283%, for (57)Fe, (112)Cd, (55)Mn and (63)Cu, respectively...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28333296/stepwise-evolution-of-a-buried-inhibitor-peptide-over-45-my
#20
Achala S Jayasena, Mark F Fisher, Jose L Panero, David Secco, Kalia Bernath-Levin, Oliver Berkowitz, Nicolas L Taylor, Edward E Schilling, James Whelan, Joshua S Mylne
The de novo evolution of genes and the novel proteins they encode has stimulated much interest in the contribution such innovations make to the diversity of life. Most research on this de novo evolution focuses on transcripts, so studies on the biochemical steps that can enable completely new proteins to evolve and the time required to do so have been lacking. Sunflower Preproalbumin with SFTI-1 (PawS1) is an unusual albumin precursor because in addition to producing albumin it also yields a potent, bicyclic protease-inhibitor called SunFlower Trypsin Inhibitor-1 (SFTI-1)...
June 1, 2017: Molecular Biology and Evolution
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