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hpv cervical smear

Juliana Lasmar Ayres do Amaral, Marizeli Viana de Aragão Araújo, George Alberto da Silva Dias, Esther Iris Christina Freifrau von Ledebur, Juarez Antonio Simões Quaresma, Hellen Thais Fuzii
Cervical cancer, the second most common cancer affecting women in Northern Brazil, is strongly associated with human papillomavirus (HPV) infection. Diseases affecting the immune state of a patient, including autoimmune diseases like systemic lupus erythematosus (SLE), can lead to persistent HPV infection and cancer. We evaluated cervical HPV prevalence and the associated risk factors in 70 women with SLE in the city of Belém, located in Brazilian Amazon. HPV DNA was detected by PCR using primers MY9 and MY11...
August 17, 2016: Acta Reumatológica Portuguesa
Mahira Jahic, Elmir Jahic
BACKGROUND: Atypical squamous cells of undetermined significance (ASCUS) is a term that refers to inflammatory, reactive and reparative processes which are atypical and of higher level and insufficient to be classified as cervical intraepithelial lesions (CIN). AIMS: Examine of frequency of HPV infection in ASCUS lesions and regression, stagnation and progression during six-month period. SUBJECTS AND METHODS: Prospective study was conducted over a period of 3 years...
July 27, 2016: Medical Archives
Ana Raposo, Chiara Tani, José Costa, Marta Mosca
An association between immune-mediated diseases and cervical pre-malignant and malignant lesions is described, having the human papillomavirus (HPV) infection a causal role. Related studies have been generally focused on systemic lupus erythematosus (SLE) patients, but relatively to other diseases, such as rheumatoid arthritis (RA), Sjögren's syndrome (SS) and systemic sclerosis (SSc), data has not been systematically evaluated. We conducted a systematic review analysis of the literature in PubMed, including articles published until March of 2015, in patients with RA, SS, SLE and SSc, to evaluate the frequency of HPV infection, cervical dysplasia and cervical cancer, and associated factors, with particular interest on the role of glucocorticoids and immunosuppressive treatment...
April 16, 2016: Acta Reumatológica Portuguesa
Jane Shen-Gunther, Chiou-Miin Wang, Graham M Poage, Chun-Lin Lin, Luis Perez, Nancy A Banks, Tim Hui-Ming Huang
BACKGROUND: The Pap smear has remained the foundation for cervical cancer screening for over 70 years. With advancements in molecular diagnostics, primary high-risk human papillomavirus (hrHPV) screening has recently become an accepted stand-alone or co-test with conventional cytology. However, both diagnostic tests have distinct limitations. The aim of this study was to determine the association between HPV genotypes and cellular epigenetic modifications in three grades of cervical cytology for screening biomarker discovery...
2016: Clinical Epigenetics
A Wilson
National cervical screening programmes are considering a move to HPV primary screening. HPV testing requires the cervical sample to be placed in liquid based cytology (LBC) media and the triage cytology test will be carried out using LBC preparations. The resources required for conversion training from conventional smears to LBC should not be underestimated as errors can be made without the use of a structured training plan and monitoring of performance. In the UK, many labs have converted between the two main LBC technologies, Thinprep and Surepath...
October 2016: Cytopathology: Official Journal of the British Society for Clinical Cytology
KeWei Chen, YiQin Ouyang, Peter Hillemanns, Matthias Jentschke
AIM: To evaluate the analytical and clinical effectiveness of cervicovaginal self-sampling with a dry sampling device (Evalyn Brush) for high-risk human papillomavirus (hr-HPV) testing and detection of cervical disease. METHODS: The study population consisted of 101 patients from a large gynecological outpatient clinic in Shanghai referred for abnormal cervical screening results and 101 women without cervical lesions. Self-samples obtained in the clinic and physician-collected cervical specimens (reference) were stored at -20 °C for 16-18 weeks and then transferred to 20 ml of ThinPrep medium and tested for hr-HPV using a multiplex real time polymerase chain reaction assay...
September 20, 2016: Journal of Obstetrics and Gynaecology Research
Suchitra Thunga, Anusmitha Andrews, John Ramapuram, Kapaettu Satyamoorthy, Hema Kini, B Unnikrishnan, Prabha Adhikari, Prakhar Singh, Shama Prasada Kabekkodu, Samatha Bhat, Anagha Kadam, Avinash K Shetty
AIM: The aim of this study was to examine the association between CD4 count, human papilloma virus (HPV) infection, and the risk of cervical intraepithelial neoplasia among HIV-infected women. METHODS: A cross-sectional study was conducted among 104 HIV-infected women attending an antiretroviral therapy clinic. They underwent Pap smear and cervical HPV DNA testing. RESULTS: The overall prevalence of HPV infection was 57.7%. HPV 16 was the commonest genotype found (38...
September 19, 2016: Journal of Obstetrics and Gynaecology Research
Xiaotong He, Innocent O Maranga, Anthony W Oliver, Peter Gichangi, Lynne Hampson, Ian N Hampson
The oncogenic retrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in some countries although its prevalence and relationship with other sexually transmitted infections in Sub-Saharan Africa is largely unknown. A novel endpoint PCR method was used to analyse the prevalence of HTLV-1 proviral DNA in genomic DNA extracted from liquid based cytology (LBC) cervical smears and invasive cervical carcinomas (ICCs) obtained from human immunodeficiency virus-positive (HIV+ve) and HIV-negative (HIV-ve) Kenyan women...
2016: Viruses
B Laabidi, S Ben Rejeb, A Bani, N Mansouri, O Lamine, A Bouzaini, I Msakni
BACKGROUND AND OBJECTIVE: The human papillomavirus (HPV) is the main causative agent of cancerous and precancerous lesions in cervical cancers. The infection detection is performed by cytological and histological examination. However, molecular testing for HPV infection remains necessary to distinguish nonspecific inflammatory lesions from HPV-related lesions. We aimed to compare cytological and histological diagnosis of HPV lesions and to confront histological findings with molecular detection of HPV by in situ hybridization (ISH)...
October 2016: Médecine et Maladies Infectieuses
O M Kolawole, K T Olatunji, K A Durowade
BACKGROUND: Human papillomavirus (HPV) has been implicated as one of the principal causes of cervical cancer, which is the second highest cause of cancer deaths among Nigerian women. OBJECTIVE: This study was aimed at determining the presence of HPV DNA in abnormal cervical cytology of a group of women who were screened using Papanicolaou staining technique. METHODOLOGY: A total of 200 women attending the Obstetrics and Gynaecology clinic of the Federal Teaching Hospital, Ido-Ekiti, were screened by means of conventional Pap smear screening, while positive samples underwent molecular analyses by means of DNA isolation techniques and polymerase chain reaction (PCR)...
2016: Journal of Preventive Medicine and Hygiene
J Wilm, S Schüler-Toprak, O Ortmann
Screening programs for cervical cancer and breast cancer lead to a clear reduction of mortality. Starting in 2018 screening for cervical cancer will be structured as an organized program as already exists for breast cancer. In future screening for cervical cancer will be primarily performed by human papillomavirus (HPV) testing at intervals of 5 years while cytological examination (Pap smear) will also be available as an additional or alternative procedure. For breast cancer screening in Germany an annual clinical examination with palpation and mammography screening at 2‑year intervals is provided for women aged between 50 and 69 years...
September 2016: Der Pathologe
Naureen Ehsan Ilahi, Shoaib Naiyar Hashmi, Sobia Anwar, Sheeba Murad
PURPOSE: Estimation of HPV-related disease burden lies at the core of effective disease management. HPV testing is heavily reliant on its retrospective detection in archival clinical cancer samples, especially in parts of the world where HPV screening is not routinely practiced. During the last decade, valuable insights were gained through regional reports based on occasional screening of cervical smears or biopsy sections for the presence of high-risk HPV. HPV 16 and 18 were found to be predominant high-risk HPV subtypes with some regional differences and incidences of co-infections, detected mostly through PCR-based methods...
November 2016: Journal of Cancer Research and Clinical Oncology
Marta Castillo, Aurora Astudillo, Omar Clavero, Julio Velasco, Raquel Ibáñez, Silvia de Sanjosé
OBJECTIVE: The objective of this study was to describe prior negative screening history and symptoms around the time of diagnosis of incident cervical cancer (CC) cases diagnosed between 2000 and 2010 within the Asturias public health system. METHODS: Records from 374 women diagnosed with CC between 2000 and 2010 from all public hospitals in Asturias were retrieved. Clinical information, FIGO stage and all previous cytological data were extracted from clinical and histopathological records...
2016: PloS One
Callinice D Capo-Chichi, Blanche Aguida, Nicodème W Chabi, Jocelyn Acapko-Ezin, Jonas Sossah-Hiffo, Vidéhouénou K Agossou, Toussain Anagbla, Marcel Zannou, Fabien Houngbé, Ambaliou Sanni
BACKGROUND: High risk oncologic Human Papilloma Virus (HPV) is one of the leading causes of cervical cancer worldwide. We investigated HPV genotypes among women living or not with Human Immuno-deficiency Virus (HIV) in two major hospitals in the south of the republic of BENIN in the city of Cotonou. Our objective is to investigate the association of high risk-HPV to cervical dysplasia among women under stringent anti-retroviral (ARV) treatment and in controls without HIV. METHODS: The investigation was carried out within 1 year period in two groups of adult women: one group with HIV1 infection and under ARV therapy in the National University Hospital (CNHU-HKM) designated as CH group (n = 86); and one control group without HIV infection and attending the hospital Mènontin for routine gynecologic checkup and designated as ME group (n = 86)...
2016: Infectious Agents and Cancer
Mustafa Ozturk, Haldun Umudum, Ibrahim Alanbay, Mustafa Ulubay, Ulas Fidan, Murat Dede, Müfit Cemal Yenen
BACKGROUND: Current cervical cancer screening guidelines recommend a 1-year follow-up period for patients with a postmenopausal low-grade squamous intraepithelial lesion (LSIL) who are test negative for high-risk human papillomavirus (HrHPV). The aim of this study was to assess whether such patients had an increased immediate risk of high-grade squamous intraepithelial lesion. METHODS: We assessed 54 HrHPV-negative women with postmenopausal LSIL in the Department of Obstetrics and Gynecology of our hospital between 2012 and 2013...
August 16, 2016: Diagnostic Cytopathology
Toni Ricardo Martins, Cristina Mendes de Oliveira, Luciana Reis Rosa, Cristiane de Campos Centrone, Célia Luiza Regina Rodrigues, Luisa Lina Villa, José Eduardo Levi
BACKGROUND: Human Papillomavirus (HPV) genotype distribution varies according to the method of assessment and population groups. This study analyzed type-specific HPV infections among women ranging from 14-95 years old, displaying normal and abnormal cytology, from São Paulo and Barretos cities, Brazil. METHODS: Women found positive for High Risk-HPVs DNA by either the Hybrid Capture 2 (HC2) or Cobas HPV Test (n = 431) plus a random sample of 223 negative by both assays and 11 samples with indeterminate results, totalizing 665 samples, were submitted to HPV detection by the PapilloCheck test...
2016: Virology Journal
Sveinung Wergeland Sørbye, Silje Fismen, Tore Jarl Gutteberg, Elin Synnøve Mortensen, Finn Egil Skjeldestad
OBJECTIVES: To assess the performance of a 5-type human papillomavirus (HPV) messenger RNA (mRNA) test in primary screening within the framework of the Norwegian population-based screening programme. DESIGN: Nationwide register-based cohort study. SETTING: In 2003-2004, general practitioners and gynaecologists recruited 18 852 women for participation in a primary screening study with a 5-type HPV mRNA test. PARTICIPANTS: After excluding women with a history of abnormal smears and with cervical intraepithelial neoplasia grade 2 (CIN2+) before or until 3 months after screening, 11 220 women aged 25-69 years were eligible for study participation...
2016: BMJ Open
M P Singh, M Kaur, N Gupta, A Kumar, K Goyal, A Sharma, M Majumdar, M Gupta, R K Ratho
PURPOSE: Cervical cancer is the most common cancer among women in developing nations. Nearly 90% of the cases have been linked to the presence of high-risk human papillomavirus (hrHPV) types 16 and 18. The risk of cervical cancer may be high in female sex workers (FSWs) due to multiple sexual partners. This study aimed to determine the prevalence of cytological abnormalities and hrHPV types 16 and 18 in FSWs in Chandigarh, North India using the liquid-based cytology (LBC) approach. MATERIALS AND METHODS: The cervical brush samples were collected from 120 FSW and 98 age-matched healthy controls (HCs)...
July 2016: Indian Journal of Medical Microbiology
Selda Yörük, Ayla Açıkgöz, Gül Ergör
BACKGROUND: The purpose of the study is to investigate knowledge, attitudes and behaviours concerning cervical cancer, HPV and HPV vaccine of female students studying at a university in a health related department and explore variables affecting taking the vaccine. METHODS: The research group consists of female students attending a health related department in Balıkesir University. The data of this cross-sectional research was collected via surveys. RESULTS: The average total knowledge score of the students concerning risks, symptoms and screening methods of cervical cancer and HPV vaccines was 14...
2016: BMC Women's Health
Cheng-Chieh Chen, Lee-Wen Huang, Chyi-Huey Bai, Chin-Cheng Lee
BACKGROUND: The Papanicolaou (Pap) test is one screening strategy used to prevent cervical cancer in developed countries. The p16/Ki-67 immunocytochemistry is a triage test performed on Pap smears in women with atypical squamous cells of undetermined significance (ASCUS) or low grade squamous intraepithelial lesion. OBJECTIVE: Our objective was to review studies investigating the diagnostic performance of p16/Ki-67 dual stain for triage of women with abnormal Pap tests...
July 2016: Annals of Saudi Medicine
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