keyword
MENU ▼
Read by QxMD icon Read
search

Laryngotracheal

keyword
https://www.readbyqxmd.com/read/28434824/multiple-pathogen-biomarker-detection-using-an-encoded-bead-array-in-droplet-pcr
#1
Prem Kumar Periyannan Rajeswari, Lovisa M Soderberg, Alia Yacoub, Mikael Leijon, Helene Andersson Svahn, Haakan N Joensson
We present a droplet PCR workflow for detection of multiple pathogen DNA biomarkers using fluorescent color-coded Luminex® beads. This strategy enables encoding of multiple singleplex droplet PCRs using a commercially available bead set of several hundred distinguishable fluorescence codes. This workflow provides scalability beyond the limited number offered by fluorescent detection probes such as TaqMan probes, commonly used in current multiplex droplet PCRs. The workflow was validated for three different Luminex bead sets coupled to target specific capture oligos to detect hybridization of three microorganisms infecting poultry: avian influenza, infectious laryngotracheitis virus and Campylobacter jejuni...
April 20, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28424763/airway-problems-in-neonates-a-review-of-the-current-investigation-and-management-strategies
#2
REVIEW
Quen Mok
Airway problems in the neonatal population are often life threatening and raise challenging issues in diagnosis and management. The airway problems can result from congenital or acquired lesions and can be broadly classified into those causing obstruction or those due to an abnormal "communication" in the airway. Many different investigations are now available to identify the diagnosis and quantify the severity of the problem, and these tests can be simple or invasive. Bronchography and bronchoscopy are essential to determine the extent and severity of the airway problem and to plan treatment strategy...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/28397558/measurement-of-cough-aerodynamics-in-healthy-adults
#3
Aaron J Feinstein, Zhaoyan Zhang, Dinesh K Chhetri, Jennifer Long
IMPORTANCE: Cough is a critical human reflex and also among the most frequent symptoms in medicine. Despite the prevalence of disordered cough in laryngeal pathologies, comprehensive and quantitative evaluation of cough in these patients is lacking. OBJECTIVE: Herein we seek to establish normative values for cough aerodynamics to provide a population standard for reference in future studies. DESIGN, SETTING, AND PARTICIPANTS: Healthy subjects were recruited from an outpatient clinic to perform voluntary cough...
May 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28384898/primary-management-and-outcome-open-laryngotracheal-trauma
#4
Santosh Uttarkar Pandurangarao, Prashanth Basavaraj, Supriya Baswaraj Yempalle, Lavanya Karnam
Acute external injury to the larynx is both life threatening and a potential long term management challenge. As Otorhinolaryngologist we must be prepared and well versed to manage these patients. In our study seven patients of open laryngeal traumas were managed by primary closure. In five patients nature of injury was known in other two patients exact nature of injury was not known. After primary closure five patients with known injury survived and two patients with unknown injury died. As per our experience, we recommend primary closure, if the exact nature of injury is known as the outcome is definitely favourable...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28350819/development-and-application-of-a-taqman-single-nucleotide-polymorphism-genotyping-assay-to-study-infectious-laryngotracheitis-virus-recombination-in-the-natural-host
#5
Carlos A Loncoman, Carol A Hartley, Mauricio J C Coppo, Paola K Vaz, Andrés Diaz-Méndez, Glenn F Browning, Sang-Won Lee, Joanne M Devlin
To date, recombination between different strains of the avian alphaherpesvirus infectious laryngotracheitis virus (ILTV) has only been detected in field samples using full genome sequencing and sequence analysis. These previous studies have revealed that natural recombination is widespread in ILTV and have demonstrated that recombination between two attenuated ILTV vaccine strains generated highly virulent viruses that produced widespread disease within poultry flocks in Australia. In order to better understand ILTV recombination, this study developed a TaqMan single nucleotide polymorphism (SNP) genotyping assay to detect recombination between two field strains of ILTV (CSW-1 and V1-99 ILTV) under experimental conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28349784/fibroblasts-in-hypoxic-conditions-mimic-laryngotracheal-stenosis
#6
Linda X Yin, Kevin M Motz, Idris Samad, Madhavi Duvvuri, Michael Murphy, Dacheng Ding, Alexander T Hillel
Objective To elucidate the role of hypoxia and inflammatory pathways in the pathogenesis of iatrogenic laryngotracheal stenosis (iLTS). Study Design (1) Examination of mucosal surface gene expression in human iLTS. (2) In vitro comparison of normal and scar laryngotracheal fibroblasts under normoxic and hypoxic conditions. Setting Tertiary care hospital in a research university (2012-2016). Subjects and Methods Brush biopsies were obtained from normal laryngotracheal tissue and scar in iLTS patients; gene expression was compared...
March 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28344898/the-non-recurrent-laryngeal-nerve-a-meta-analysis-and-clinical-considerations
#7
Brandon Michael Henry, Silvia Sanna, Matthew J Graves, Jens Vikse, Beatrice Sanna, Iwona M Tomaszewska, R Shane Tubbs, Jerzy A Walocha, Krzysztof A Tomaszewski
BACKGROUND: The Non-Recurrent Laryngeal Nerve (NRLN) is a rare embryologically-derived variant of the Recurrent Laryngeal Nerve (RLN). The presence of an NRLN significantly increases the risk of iatrogenic injury and operative complications. Our aim was to provide a comprehensive meta-analysis of the overall prevalence of the NRLN, its origin, and its association with an aberrant subclavian artery. METHODS: Through March 2016, a database search was performed of PubMed, CNKI, ScienceDirect, EMBASE, BIOSIS, SciELO, and Web of Science...
2017: PeerJ
https://www.readbyqxmd.com/read/28287060/end-to-end-anastomosis-in-the-management-of-laryngotracheal-defects
#8
E D Gozen, M Yener, Z B Erdur, E Karaman
OBJECTIVE: To present clinical experience and surgical outcomes of end-to-end anastomosis in the management of laryngotracheal stenosis and tracheal defects following invasive thyroid malignancy resection. METHODS: A retrospective analysis was performed of 14 patients with laryngotracheal stenosis and tracheal invasive thyroid malignancy. All patients underwent tracheal or cricotracheal resection and primary end-to-end anastomosis. RESULTS: Length of stenosis was 1...
May 2017: Journal of Laryngology and Otology
https://www.readbyqxmd.com/read/28277335/voice-and-swallowing-outcomes-for-adults-undergoing-reconstructive-surgery-for-laryngotracheal-stenosis
#9
Gemma M Clunie, Andrew J Kinshuck, Gurpreet S Sandhu, Justin W G Roe
PURPOSE OF REVIEW: Adult laryngotracheal stenosis is a rare, multifactorial condition which carries a significant physical and psychosocial burden. Surgical approaches have developed in recent years, however, voice and swallowing function can be affected prior to treatment, in the immediate postoperative phase, and as an ongoing consequence of the condition and surgical intervention. In this study we discuss: the nature of the problem; surgical interventions to address airway disorders; optimal patterns of care to maximize voice and swallowing outcomes...
March 8, 2017: Current Opinion in Otolaryngology & Head and Neck Surgery
https://www.readbyqxmd.com/read/28239579/neck-trauma-ent-prospects
#10
Kavita Sachdeva, Aparaajita Upadhyay
Neck trauma is a very important surgical emergency faced by ENT surgeons in day to day practice. They are potentially life threatening conditions due to the presence of many vital structures in this area. Timely presentation to the referral centre and proper multidisciplinary approach towards management plays a pivotal role in the healing pattern of the wound and prevention of serious complication like shock, sepsis, laryngeal stenosis or fistula formation. A retrospective study was done in ENT Department, NSCB Medical College, Jabalpur, Madhya Pradesh, India during the period of 2014-2016...
March 2017: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/28194527/impairment-of-infectious-laryngotracheitis-virus-replication-by-deletion-of-the-ul-1-gene
#11
M Nadimpalli, S W Lee, J M Devlin, J R Gilkerson, C A Hartley
Infectious laryngotracheitis virus (ILTV) encodes several unique genes, including a pair of unique nuclear proteins UL0 and UL[-1] that are expressed during replication in cell culture. Although the UL0 gene has been shown to be dispensable for replication, the role of UL[-1] has not been elucidated. In this study a deletion mutant of ILTV lacking the UL[-1] gene was constructed using homologous recombination. The coding sequences of the gene were replaced with the gene for enhanced green fluorescent protein and the cytomegalovirus major immediate early promoter element...
February 13, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28167015/external-airway-splint-to-treat-tracheomalacia-following-laryngotracheal-reconstruction
#12
Wayne D Hsueh, Lee P Smith
This observation reports the use of an external airway splint to treat tracheomalacia in a pediatric patient. The patient underwent a double stage laryngotracheal reconstruction however was unable to be decannulated due to severe tracheomalacia. Our purpose is to further support the use of external splinting in the treatment of tracheomalacia in a unique case involving isolated nighttime airway obstruction following laryngotracheal reconstruction.
March 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28163937/a-narrowing-diagnosis-a-rare-cause-of-adult-croup-and-literature-review
#13
Jayshil J Patel, Emily Kitchin, Kurt Pfeifer
Croup or laryngotracheitis is rare in adults. We present a case of an otherwise healthy young woman that presented in the winter with 3 days of increasing dyspnea, cough, and fever. She was hemodynamically stable but was found to have a barking cough, paradoxical abdominal breathing, and stridor. Chest radiograph revealed subglottic narrowing. Respiratory viral nucleic acid amplification testing was positive for respiratory syncytial virus. The patient was treated with nebulized epinephrine, dexamethasone, and a helium-oxygen mixture...
2017: Case Reports in Critical Care
https://www.readbyqxmd.com/read/28151004/evaluation-of-factors-influencing-the-development-of-late-marek-s-disease-virus-induced-immunosuppression-virus-pathotype-and-host-sex
#14
Nik M Faiz, Aneg L Cortes, James S Guy, Oscar J Fletcher, Thomas Cimino, Isabel M Gimeno
Marek's disease virus (MDV) is a herpesvirus that induces lymphoma and immunosuppression in chickens. MDV-induced immunosuppression (MDV-IS) is complex and can be divided into two phases: early-MDV-IS associated with cytolytic infection in the lymphoid organs in chickens lacking maternal antibodies against MDV (MAbs) and late-MDV-IS that appears later in the pathogenesis and occurs even in chickens bearing MAbs. We have recently developed a model to reproduce late-MDV-IS under laboratory conditions. This model evaluates late-MDV-IS indirectly by assessing the effect of MDV infection on the efficacy of infectious laryngotracheitis (ILT) vaccines against challenge with ILT virus...
April 24, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28132084/pediatric-airway-surgery
#15
REVIEW
Kosaku Maeda
Pediatric airway surgery is a challenging field in pediatric surgery. Laryngotracheal stenosis has a variety of congenital and acquired conditions that require precise assessment and tailored treatment for each individual patient. About 90% of acquired conditions are represented by subglottic stenosis (SGS) resulting as a complication of tracheal intubation. Congenital tracheal stenosis (CTS) is a rare and life-threatening malformation, usually associated with complete tracheal rings along a variable length of the trachea...
April 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28124628/comparison-of-swallowing-outcomes-of-laryngotracheal-separation-versus-total-laryngectomy-in-a-validated-ovine-model-of-profound-oropharyngeal-dysphagia
#16
COMPARATIVE STUDY
N N Venkatesan, C M Johnson, M T Siddiqui, D J Cates, M A Kuhn, G N Postma, P C Belafsky
OBJECTIVES: To validate the ovine model of profound oropharyngeal dysphagia and compare swallowing outcomes of laryngotracheal separation with those of total laryngectomy. METHODS: Under real-time fluoroscopy, swallowing trials were conducted using the head and neck of two Dorper cross ewes and one human cadaver, secured in lateral fluoroscopic orientation. Barium trials were administered at baseline, pre- and post-laryngohyoid suspension, following laryngotracheal separation, and following laryngectomy in the ovine model...
April 2017: Journal of Laryngology and Otology
https://www.readbyqxmd.com/read/28122110/invasive-fungal-laryngopharyngitis-resulting-in-laryngeal-destruction-with-complete-laryngotracheal-separation-report-of-a-case
#17
Tyler Swiss, Sergio Santino Cervantes, Michael Hinni, David G Lott
As the treatment of hematopoietic cancers evolves, otolaryngologists will see a higher incidence of opportunistic infections. We discuss a case of invasive fungal disease that invaded the larynx, pharynx, trachea, and pulmonary parenchyma after chemotherapy. The patient, a 46-year-old woman, presented 1 week after undergoing induction chemotherapy. Her initial symptoms were odynophagia and dysphagia. Despite encouraging findings on physical examination, her health rapidly declined and she required an urgent tracheotomy and multiple operations to address spreading necrosis...
January 2017: Ear, Nose, & Throat Journal
https://www.readbyqxmd.com/read/28112014/laryngotracheal-stenosis
#18
Shekhar K Gadkaree, Vinciya Pandian, Simon Best, Kevin M Motz, Clint Allen, Young Kim, Lee Akst, Alexander T Hillel
Objective Laryngotracheal stenosis (LTS) is a fibrotic process that narrows the upper airway and has a significant impact on breathing and phonation. Iatrogenic injury from endotracheal and/or tracheostomy tubes is the most common etiology. This study investigates differences in LTS etiologies as they relate to tracheostomy dependence and dilation interval. Study Design Case series with chart review. Setting Single-center tertiary care facility. Subjects and Methods Review of adult patients with LTS was performed between 2004 and 2015...
February 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28109571/in%C3%A2-vivo-tissue-engineering-of-human%C3%A2-airways
#19
Emmanuel Martinod, Joseph Paquet, Hervé Dutau, Dana M Radu, Morad Bensidhoum, Sébastien Abad, Yurdagül Uzunhan, Eric Vicaut, Hervé Petite
BACKGROUND: Airway transplantation remains a major challenge in thoracic surgery. Based on our previous laboratory work, we developed the techniques required to bioengineer a tracheal substitute in vivo using cryopreserved aortic allografts as biological matrices (Replacement of the Airways and/or the Pulmonary Vessels Using a Cryopreserved Arterial Allograft [TRACHEOBRONCART] Study, NCT01331863). We present here 2 patients who had a definitive tracheostomy for complex laryngotracheal stenoses refractory to conventional therapy...
January 18, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28109507/surgery-of-the-head-and-neck-in-patient-with-kniest-dysplasia-is-wound-healing-an-issue
#20
Qasim Husain, Jungsuk Cho, Jay Neugarten, Vikash K Modi
Kniest dysplasia is a type II collagen disorder that arises from a genetic mutation of the COL2A1 gene that results in short stature, midface anomalies, tracheomalacia, and hearing loss. Disruption of the normal collagen pathway can lead to many changes given its critical role in the body, and can cause complications with respect to wound healing. We present a case in which a patient with Kniest dysplasia successfully underwent multiple procedures in the head and neck region including cochlear implantation, mandibular distraction, palatoplasty, and laryngotracheal reconstruction...
February 2017: International Journal of Pediatric Otorhinolaryngology
keyword
keyword
98764
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"