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https://www.readbyqxmd.com/read/29230245/adaptation-and-molecular-characterization-of-two-malaysian-very-virulent-infectious-bursal-disease-virus-isolates-adapted-in-bgm-70-cell-line
#1
Nafi'u Lawal, Mohd Hair-Bejo, Siti Suri Arshad, Abdul Rahman Omar, Aini Ideris
Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 and UPM190 (also known as UPMB00/81 and UPM04/190, respectively) isolated from local IBD outbreaks were serially passaged 12 times (EP12) in specific pathogen free (SPF) chicken embryonated eggs (CEE) by chorioallantoic membrane (CAM) route. The EP12 isolate was further adapted and serially propagated in BGM-70 cell line up to 20 passages (P20). Characteristic cytopathic effects (CPEs) were subtly observed at P1 in both isolates 72 hours postinoculation (pi)...
2017: Advances in Virology
https://www.readbyqxmd.com/read/29208259/the-role-of-a-novel-long-noncoding-rna-tuc40-in-cardiomyocyte-induction-and-maturation-in-p19-cells
#2
Huijuan Li, Li Jiang, Zhangbin Yu, Shuping Han, Xuehua Liu, Mengmeng Li, Chun Zhu, Lixing Qiao, Li Huang
BACKGROUND: In previous studies, TUC40-, a new long noncoding RNA, was found to be overexpressed in human ventricular septal defect (VSD) embryonic heart samples. In this article, we carried out experiments on the P19 cell line to elucidate the effects of TUC40- overexpression on cardiomyocyte development relevant to VSD pathogenesis. METHODS: We established the overexpression cell model by plasmid transfection, and explored the expression profile of Pbx1, the sense gene of TUC40-, and the marker genes of cardiomyocyte linage commitment (Nkx2...
December 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/29207191/novel-evidence-that-pituitary-sex-hormones-regulate-migration-adhesion-and-proliferation-of-embryonic-stem-cells-and-teratocarcinoma-cells
#3
Zachariah Payne Sellers, Kamila Bujko, Gabriela Schneider, Magdalena Kucia, Mariusz Z Ratajczak
The pituitary sex hormones (SexHs): follicle‑stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) regulate several functions crucial for reproduction, including oogenesis, spermatogenesis, and lactation. An important source of prolactin-like hormones, known as lactogens, is the placenta, and lactogens bind to the PRL receptor (PRLR) with high affinity and thereby mimic the actions of PRL. Recently, it has been demonstrated that pituitary SexHs were involved in metastatic lung cancer, certain sarcomas, and leukemia...
November 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29168048/homozygous-expression-of-mutant-elovl4-leads-to-seizures-and-death-in-a-novel-animal-model-of-very-long-chain-fatty-acid-deficiency
#4
Blake R Hopiavuori, Ferenc Deák, Joseph L Wilkerson, Richard S Brush, Nicole A Rocha-Hopiavuori, Austin R Hopiavuori, Kathryn G Ozan, Michael T Sullivan, Jonathan D Wren, Constantin Georgescu, Luke Szweda, Vibhudutta Awasthi, Rheal Towner, David M Sherry, Robert E Anderson, Martin-Paul Agbaga
Lipids are essential components of the nervous system. However, the functions of very long-chain fatty acids (VLC-FA; ≥ 28 carbons) in the brain are unknown. The enzyme ELOngation of Very Long-chain fatty acids-4 (ELOVL4) catalyzes the rate-limiting step in the biosynthesis of VLC-FA (Agbaga et al., Proc Natl Acad Sci USA 105(35): 12843-12848, 2008; Logan et al., J Lipid Res 55(4): 698-708, 2014), which we identified in the brain as saturated fatty acids (VLC-SFA). Homozygous mutations in ELOVL4 cause severe neuropathology in humans (Ozaki et al...
November 22, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/29121352/arsenic-induces-members-of-the-mmu-mir-466-669-cluster-which-reduces-neurod1-expression
#5
Jui-Tung Liu, Lisa J Bain
Chronic arsenic exposure can result in adverse development effects including decreased intellectual function, reduced birth weight, and altered locomotor activity. Previous in vitro studies have shown that arsenic inhibits stem cell differentiation. MicroRNAs (miRNAs) are small non-coding RNAs that regulate multiple cellular processes including embryonic development and cell differentiation. The purpose of this study was to examine whether altered miRNA expression was a mechanism by which arsenic inhibited cellular differentiation...
November 7, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29112861/long-fiber-carbon-nanotubes-replicate-asbestos-induced-mesothelioma-with-disruption-of-the-tumor-suppressor-gene-cdkn2a-ink4a-arf
#6
Tatyana Chernova, Fiona A Murphy, Sara Galavotti, Xiao-Ming Sun, Ian R Powley, Stefano Grosso, Anja Schinwald, Joaquin Zacarias-Cabeza, Kate M Dudek, David Dinsdale, John Le Quesne, Jonathan Bennett, Apostolos Nakas, Peter Greaves, Craig A Poland, Ken Donaldson, Martin Bushell, Anne E Willis, Marion MacFarlane
Mesothelioma is a fatal tumor of the pleura and is strongly associated with asbestos exposure. The molecular mechanisms underlying the long latency period of mesothelioma and driving carcinogenesis are unknown. Moreover, late diagnosis means that mesothelioma research is commonly focused on end-stage disease. Although disruption of the CDKN2A (INK4A/ARF) locus has been reported in end-stage disease, information is lacking on the status of this key tumor suppressor gene in pleural lesions preceding mesothelioma...
November 6, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29109239/graded-elevation-of-c-jun-in-schwann-cells-in-vivo-gene-dosage-determines-effects-on-development-re-myelination-tumorigenesis-and-hypomyelination
#7
Shaline V Fazal, Jose A Gomez-Sanchez, Laura J Wagstaff, Nicolo Musner, Georg Otto, Martin Janz, Rhona Mirsky, Kristjan R Jessen
Schwann cell c-Jun is implicated in adaptive and maladaptive functions in peripheral nerves. In injured nerves, this transcription factor promotes the repair Schwann cell phenotype and regeneration, and it promotes Schwann cell mediated neurotrophic support in models of peripheral neuropathies. However, c-Jun is associated with tumour formation in some systems, it potentially supresses myelin genes, and has been implicated in demyelinating neuropathies. To clarify these issues, and determine how c-Jun levels determine its function, we have generated, c-Jun OE/+ and c-Jun OE/OE mice, with graded expression of c-Jun in Schwann cells, and examined these lines during development, in adulthood and after injury using RNA sequencing analysis, quantitative electron microscopic morphometry, Western blotting and functional tests...
November 6, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29104531/the-repression-of-atoh1-by-neurogenin1-during-inner-ear-development
#8
Héctor Gálvez, Juan J Tena, Fernando Giraldez, Gina Abelló
Atonal homolog 1 (Atoh1) and Neurogenin1 (Neurog1) are basic Helix-Loop-Helix (bHLH) transcription factors crucial for the generation of hair cells (HCs) and neurons in the inner ear. Both genes are induced early in development, but the expression of Atoh1 is counteracted by Neurog1. As a result, HC development is prevented during neurogenesis. This work aimed at understanding the molecular basis of this interaction. Atoh1 regulation depends on a 3'Atoh1-enhancer that is the site for Atoh1 autoregulation. Reporter assays on chick embryos and P19 cells show that Neurog1 hampers the autoactivation of Atoh1, the effect being cell autonomous and independent on Notch activity...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29066476/role-of-cdkn2c-fluorescence-in-situ-hybridization-in-the-management-of-medullary-thyroid-carcinoma
#9
Maha El Naofal, Adriel Kim, Hui Yi Yon, Mohamed Baity, Zhao Ming, Jacquelin Bui-Griffith, Zhenya Tang, Melissa Robinson, Elizabeth G Grubbs, Gilbert J Cote, Peter Hu
Medullary thyroid carcinoma (MTC), an aggressive form of thyroid cancer, occurs sporadically in approximately 75% of MTCs. RET and RAS mutations play a role in about 40% and 15%, respectively, of sporadic MTCs and are predominant drivers in MTC pathways. These mutations are some of the most comprehensively described and screened for in MTC patients; however, in recent studies, other mutations in the CDKN2C gene (p18) have been implicated in the tumorigenesis of MTC. Comparative genomic hybridization analysis revealed that approximately 40% of sporadic MTC samples have loss of CDKN2C at chromosome 1p32 in addition to frequent losses of CDKN2D (p19) at chromosome 19p13...
September 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29055088/the-transcriptome-extracellular-proteome-and-active-secretome-of-agroinfiltrated-n-benthamiana-uncover-a-large-diverse-protease-repertoire
#10
Friederike Grosse-Holz, Steven Kelly, Svenja Blaskowski, Farnusch Kaschani, Markus Kaiser, Renier A L van der Hoorn
Infiltration of disarmed Agrobacterium tumefaciens into leaves of Nicotiana benthamiana (agroinfiltration) facilitates quick and safe production of antibodies, vaccines, enzymes and metabolites for industrial use (Molecular Farming). However, yield and purity of proteins produced by agroinfiltration are hampered by unintended proteolysis, restricting industrial viability of the agroinfiltration platform. Proteolysis may be linked to an immune response to agroinfiltration, but understanding of the response to agroinfiltration is limited...
October 21, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29043884/guselkumab-for-psoriasis-a-critical-appraisal-of-phase-iii-studies
#11
Zenas Zn Yiu, Richard B Warren
Biologic therapies have raised the frontiers of accepted treatment efficacy for severe psoriasis. Guselkumab is an IgG1 monoclonal antibody that binds to the p19 subunit and inhibits IL-23. In three Phase III randomized, active comparator and placebo controlled trials, guselkumab demonstrated superior efficacy and a comparable safety profile when assessed against adalimumab and ustekinumab. Critical appraisal highlighted uncertainties over risk of bias from missing details in the trial publications that would be overcome with the provision of accompanying trial protocols, as well as the need for a head-to-head trial against an IL-17 inhibitor...
October 18, 2017: Immunotherapy
https://www.readbyqxmd.com/read/29042507/selection-of-functional-2a-sequences-within-foot-and-mouth-disease-virus-requirements-for-the-npgp-motif-with-a-distinct-codon-bias
#12
Jonas Kjær, Graham J Belsham
Foot-and-mouth disease virus (FMDV) has a positive-sense ssRNA genome including a single, large, open reading frame. Splitting of the encoded polyprotein at the 2A/2B junction is mediated by the 2A peptide (18 residues long) which induces a non-proteolytic, co-translational, "cleavage" at its own C-terminus. A conserved feature among variants of 2A is the C-terminal motif N16P17G18/P19 where P19 is the first residue of 2B. It has been shown previously that certain amino acid substitutions can be tolerated at residues E14, S15 and N16 within the 2A sequence of infectious FMDVs but no variants at residues P17, G18 or P19 have been identified...
October 17, 2017: RNA
https://www.readbyqxmd.com/read/29040919/functional-analyses-of-the-interaction-of-chicken-interleukin-23-subunit-p19-with-il-12-subunit-p40-to-form-the-il-23-complex
#13
Anh Duc Truong, Cong Thanh Hoang, Yeojin Hong, Janggeun Lee, Kyungbaek Lee, Hyun S Lillehoj, Yeong Ho Hong
This study represents the first description of the cloning of chicken IL-23p19 (ChIL-23α) and the function of the IL-23 complex in birds. Multiple alignment of ChIL-23α with other known IL-23α amino acid sequences revealed regions of amino acid conservation. The homologies of ChIL-23α, IL-12p35, and similar mammalian subunits ranged between 26% and 42%. ChIL-23α consisted of four exons and three introns; similar to those in humans and mice, and limited conservation of synteny between the human and chicken genomes was observed...
October 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29024769/identification-of-a-single-p19-gene-and-three-p40-paralogues-in-grass-carp-ctenopharyngodon-idellus-their-potential-for-the-formation-of-interleukin-23-and-inducible-expression-in-vitro-and-in-vivo
#14
Xinyan Wang, Lei Qin, Linyong Du, Di Chen, Anying Zhang, Kun Yang, Hong Zhou
Interleukin (IL-) 23, a member of IL-12 family, is a composite cytokine with the subunits of p19 and p40. Although IL-12 and IL-23 share the p40 subunit, they play vastly different roles in immune regulation. In teleost, much emphasis has been placed on the identification of IL-12, but evidence for the existence of IL-23 is still lacking. In the present study, a p19 gene and three p40 paralogues were isolated and identified from grass carp, suggesting multiple assembly of IL-23 molecules in fish species. To address this issue, the existence of different p19/p40 heterodimers were examined by Co-Immunoprecipitation (Co-IP) assay, showing that only co-expression of p19 and each p40 subunit could produce the soluble proteins corresponding to three IL-23 isoforms...
October 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28994166/newer-treatments-of-psoriasis-regarding-il-23-inhibitors-phosphodiesterase-4-inhibitors-and-janus-kinase-inhibitors
#15
REVIEW
Dominika Wcisło-Dziadecka, Martyna Zbiciak-Nylec, Ligia Brzezińska-Wcisło, Katarzyna Bebenek, Agata Kaźmierczak
The rapid progress of genetic engineering furthermore opens up new prospects in the therapy of this difficult-to-treat disease. IL-23 inhibitors, phosphodiesterase 4 (PDE4) inhibitors, and Janus kinase (JAK) inhibitors are currently encouraging further research. Two drugs which are IL-23 inhibitors are now in phase III of clinical trials. The aim of the action of both drugs is selective IL-23 inhibition by targeting the p19 subunit. Guselkumab is a fully human monoclonal antibody. Tildrakizumab is a humanized monoclonal antibody, which also belongs to IgG class and is targeted to subunit p19 of interleukin 23 (IL-23)...
October 10, 2017: Dermatologic Therapy
https://www.readbyqxmd.com/read/28967672/link-between-embryonic-stem-cell-pluripotency-and-homologous-allelic-pairing-of-oct4-loci
#16
Masamitsu N Asaka, Kousuke Uranishi, Ayumu Suzuki, Masataka Hirasaki, Masazumi Nishimoto, Akihiko Okuda
The Oct4 gene is a master regulator of the pluripotent properties of embryonic stem cells (ESCs). Recently, Oct4 loci were shown to frequently localize in close proximity to one another during the early stage of cellular differentiation, implicating this event as an important prerequisite step for ESCs to exert their full differentiation potential. Although the differentiation capacity of embryonal carcinoma cells (ECCs), such as F9 and P19 ECC lines, is severely restricted compared with ESCs, ECCs bear a highly similar expression profile to that of ESCs including expression of Oct4 and other pluripotency marker genes...
October 2, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28962207/whole-brain-ct-perfusion-imaging-using-increased-sampling-intervals-a-pilot-study
#17
Guoquan Cao, Weijian Chen, Houzhang Sun, Xianzhong Guo, Yunjun Yang, Kun Tang, Jinjin Liu
The aim of the present study was to investigate the feasibility of whole-brain perfusion imaging using the increased sampling interval protocol for 320-detector row dynamic-volume computed tomography (CT). A total of 12 volunteers were recruited. The novel protocols with 11 volumes (defined as protocol P11) and 15 volumes (defined as protocol P15) were performed on the volunteers to evaluate whether P11 and P15 are able to acquire comparable results to the standard protocol with 19 volumes (defined as protocol P19) according to the as-low-as-reasonably-achievable principle...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28949402/gain-of-function-in-the-mouse-model-of-a-recurrent-mutation-p53-n236s-promotes-the-formation-of-double-minute-chromosomes-and-the-oncogenic-potential-of-p19-arf
#18
Lanjun Zhao, Boyuan Wang, Xilong Zhao, Xiaoming Wu, Qiushi Zhang, Chuanyu Wei, Minling Shi, Yunlong Li, Wenru Tang, Jihong Zhang, Julun Yang, Sanjay Kumar Singh, Shuting Jia, Ying Luo
The mutation p53(N236S) (p53S) has been identified as one of the recurrent mutations in human cancers by TCGA database. Our in vitro data revealed the oncogenic gain of function of p53S. To understand the function of p53S in vivo, we generated the p53S knock-in mouse. The p53(S/S) mice manifested highly invasive lymphomas and metastatic sarcomas with dramatically increased double minute chromosomes. The survival curve, the incidence of tumors and the tumor spectrum of p53(S/S) mice is very similar to the p53(R172H) mouse model...
September 26, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28940259/efficacy-of-guselkumab-in-subpopulations-of-patients-with-moderate-to-severe-plaque-psoriasis-a-pooled-analysis-of-the-phase-3-voyage-1-and-voyage-2-studies
#19
K B Gordon, A Blauvelt, P Foley, M Song, Y Wasfi, B Randazzo, Y K Shen, Y You, C E M Griffiths
OBJECTIVES: To evaluate the efficacy of guselkumab in patient subgroups with moderate-to-severe psoriasis from the pooled guselkumab VOYAGE 1 and VOYAGE 2 Phase 3 studies. METHODS: Using data from the pooled VOYAGE 1 and VOYAGE 2 psoriasis studies, analyses were performed to evaluate the consistency of efficacy (Investigator's Global Assessment [IGA] 0/1 [cleared or minimal psoriasis] and IGA 0 [cleared]) across subpopulations defined by demographics, baseline disease characteristics, and previous psoriasis treatment...
September 22, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28933666/regulation-of-proliferation-in-developing-human-tooth-germs-by-msx-homeodomain-proteins-and-cyclin-dependent-kinase-inhibitor-p19ink4d
#20
Darko Kero, Katarina Vukojevic, Petra Stazic, Danijela Sundov, Snjezana Mardesic Brakus, Mirna Saraga-Babic
Before the secretion of hard dental tissues, tooth germs undergo several distinctive stages of development (dental lamina, bud, cap and bell). Every stage is characterized by specific proliferation patterns, which is regulated by various morphogens, growth factors and homeodomain proteins. The role of MSX homeodomain proteins in odontogenesis is rather complex. Expression domains of genes encoding for murine Msx1/2 during development are observed in tissues containing highly proliferative progenitor cells. Arrest of tooth development in Msx knockout mice can be attributed to impaired proliferation of progenitor cells...
October 2, 2017: Organogenesis
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