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https://www.readbyqxmd.com/read/29777679/development-of-dnazyme-based-pcr-signal-cascade-amplification-for-visual-detection-of-listeria-monocytogenes-in-food
#1
Zhanmin Liu, Chenhui Yao, Cuiyun Yang, Yanming Wang, Sibao Wan, Junyi Huang
Listeria monocytogenes is an important food-borne pathogen, and it can cause severe diseases. Rapid detection of L. monocytogenes is crucial to control this pathogen. A simple and robust strategy based on the cascade of PCR and G-quadruplex DNAzyme catalyzed reaction was used to detect L. monocytogenes. In the presence of hemin and the aptamer formed during PCR, the catalytic horseradish peroxidase-mimicking G-quadruplex DNAzymes allow the colorimetric responses of target DNA from L. monocytogenes. This assay can detect genomic DNA of L...
May 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29770048/synopsis-lyme-disease-in-canada-a-federal-framework
#2
(no author information available yet)
The Federal Framework on Lyme Disease Act required the federal government to develop a Federal Framework on Lyme Disease. To do this, the Public Health Agency of Canada held a conference with a wide variety of stakeholders who shared their experiences with Lyme disease, and discussed current knowledge and research. A draft version of the Framework was publicly posted on Canada.ca for a 30-day public consultation period. The final report, Lyme Disease in Canada: A Federal Framework was released in May 2017. The Framework includes the three pillars of surveillance; education and awareness; and guidelines and best practices...
October 5, 2017: Canada Communicable Disease Report, Relevé des Maladies Transmissibles Au Canada
https://www.readbyqxmd.com/read/29753341/human-parasitology-and-parasitic-diseases-heading-towards-2050
#3
Peter J Hotez
By 2050 our civilized planet may be comprised predominantly of networked megacities embedded in warm subtropical and tropical climates, and under stress from climate change and catastrophic weather events. Urban slum areas in these cities, including those found in wealthier middle- and high-income nations (blue marble health), will be especially vulnerable to disease. Moreover, regional conflicts fought over shifting and limited resources, including water, will collapse health systems infrastructures to further promote disease emergence and reemergence...
2018: Advances in Parasitology
https://www.readbyqxmd.com/read/29748713/occurrence-of-selected-zoonotic-food-borne-parasites-and-first-molecular-identification-of-alaria-alata-in-wild-boars-sus-scrofa-in-italy
#4
Alessia Libera Gazzonis, Luca Villa, Katharina Riehn, Ahmad Hamedy, Stefano Minazzi, Emanuela Olivieri, Sergio Aurelio Zanzani, Maria Teresa Manfredi
Wild boar is a source of human infections with zoonotic pathogens, including food-borne parasites. With the aim of a characterization of the human exposure risk, a survey on wild boars intended for human consumption was planned, selecting three pathogens, Toxoplasma gondii, Alaria alata, and Trichinella spp., as markers of meat infection. Diaphragm muscle samples from 100 wild boars hunted in Piedmont region (Northern Italy) in two hunting seasons (2015-2016) were collected. Concerning T. gondii, a combined approach of antibody detection and molecular techniques with genotyping was performed...
May 11, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29743464/-quantitative-analysis-of-unicapsula-seriolae-in-greater-amberjack-associated-with-unidentified-food-borne-disease
#5
Takahiro Ohnishi, Tetuya Obara, Sakura Arai, Tomoya Yoshinari, Yoshiko Sugita-Konishi
It has been suggested that a myxosporean parasite, Unicapsula seriolae, is responsible for food-borne disease associated with the ingestion of raw greater amberjack. In this study, we quantified U. seriolae in greater amberjack meats involved in food-poisoning episodes. U. seriolae DNA was detected in 26 samples out of 29 samples by means of quantitative real-time PCR(qRT-PCR). The major symptoms were diarrhea and vomiting within 12 hours after consumption. No seasonal trend in the outbreaks was apparent. The number of spores in samples with qRT-PCR-detected U...
2018: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/29738078/tick-borne-disease-risk-in-a-forest-food-web
#6
Richard S Ostfeld, Taal Levi, Felicia Keesing, Kelly Oggenfuss, Charles D Canham
Changes to the community ecology of hosts for zoonotic pathogens, particularly rodents, are likely to influence the emergence and prevalence of zoonotic diseases worldwide. However, the complex interactions between abiotic factors, pathogens, vectors, hosts, and both food resources and predators of hosts are difficult to disentangle. Here we (1) use 19 years of data from six large field plots in southeastern New York to compare the effects of hypothesized drivers of interannual variation in Lyme disease risk, including the abundance of acorns, rodents, and deer, as well as a series of climate variables; and (2) employ landscape epidemiology to explore how variation in predator community structure and forest cover influences spatial variation in the infection prevalence of ticks for the Lyme disease bacterium, Borrelia burgdorferi, and two other important tick-borne pathogens, Anaplasma phagocytophilum and Babesia microti...
May 8, 2018: Ecology
https://www.readbyqxmd.com/read/29735848/septicemic-listeriosis-an-emerging-food-borne-illness-in-india
#7
Angel T Miraclin, Susmitha K Perumalla, John Davis Prasad, Thambu David Sudarsanam
Listeriosis is a food borne illness of significant public health concern, caused by consumption of food contaminated by gram negative bacilli, Listeria monocytogenes. Clinical listeriosis is relatively rare and it has varying spectrum of presentation, ranging from severe sepsis in immune-compromised individuals, febrile gastroenteritis and meningo-encephalitis in infants and adults. This disease is under reported in developing nations due to the lack of awareness and inadequate laboratory facilities to promptly isolate and identify the organism...
January 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29714160/increased-human-incidence-of-west-nile-virus-disease-near-rice-fields-in-california-but-not-in-southern-united-states
#8
Tony J Kovach, A Marm Kilpatrick
Anthropogenic land use change, including agriculture, can alter mosquito larval habitat quality, increase mosquito abundance, and increase incidence of vector-borne disease. Rice is a staple food crop for more than half of the world's population, with ∼1% of global production occurring within the United States (US). Flooded rice fields provide enormous areas of larval habitat for mosquito species and may be hotspots for mosquito-borne pathogens, including West Nile virus (WNV). West Nile virus was introduced into the Americas in 1999 and causes yearly epidemics in the US with an average of approximately 1,400 neuroinvasive cases and 130 deaths per year...
April 30, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29709546/purification-of-native-sigma-class-glutathione-transferase-from-fasciola-hepatica
#9
Joshua Duncan, David Cutress, Russell M Morphew, Peter M Brophy
Fascioliasis is a parasitic disease of grazing livestock and a threat to global food security by significantly reducing the production value of sheep, goats and cattle. Moreover, the zoonotic parasite is also a re-emerging food borne threat to human populations. Driven by climate change, the prevalence of fascioliasis is set to increase. Efforts to control the main causative agent, Fasciola hepatica, are hampered by short lived chemotherapy approaches that are becoming increasingly obsolete due to therapeutic failure and resistance...
April 27, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29707545/a-review-of-bunyamwera-batai-and-ngari-viruses-understudied-orthobunyaviruses-with-potential-one-health-implications
#10
REVIEW
M Fausta Dutuze, Manassé Nzayirambaho, Christopher N Mores, Rebecca C Christofferson
Bunyamwera (BUNV), Batai (BATV), and Ngari (NRIV) are mosquito-borne viruses of the Bunyamwera serogroup in the Orthobunyavirus genus of the Bunyaviridae family. These three viruses have been found to cause disease in both livestock animals, avian species, and humans. Thus, these viruses pose a potential threat to human public health, animal health, and food security. This is especially the case in the developing nations, where BUNV and NRIV are found, mainly in Africa. BUNV and BATV are fairly well characterized, while NRIV is not well characterized owing to only sporadic detection in human and animal populations in Africa...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29706576/antifungal-activity-of-gallesia-integrifolia-fruit-essential-oil
#11
Keila Fernanda Raimundo, Wanessa de Campos Bortolucci, Jasmina Glamočlija, Marina Soković, José Eduardo Gonçalves, Giani Andrea Linde, Nelson Barros Colauto, Zilda Cristiani Gazim
Gallesia integrifolia (Phytolaccaceae) is native to Brazil and has a strong alliaceous odor. The objective of this study was to identify the chemical composition of G. integrifolia fruit essential oil and evaluate fungicidal activity against the main food-borne diseases and food spoilage fungi. The essential oil was extracted by hydrodistillation and identified by GC-MS. From 35 identified compounds, 68% belonged to the organosulfur class. The major compounds were dimethyl trisulfide (15.49%), 2,8-dithianonane (52...
April 12, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29706332/public-health-significance-of-campylobacter-spp-colonisation-of-wild-game-pheasants-phasianus-colchicus-in-scotland
#12
Alessandro Seguino, Cosmin Chintoan-Uta, Sionagh H Smith, Darren J Shaw
Campylobacter is the most common cause of bacterial food-borne diarrhoeal disease worldwide. Chicken meat is considered the main source of human infection; however, C. jejuni and C. coli have also been reported in a range of livestock and wildlife species, including pheasants. Wild pheasant meat reaches the consumer's table because of hunting but there is a lack of information concerning the risk of Campylobacter infection in humans. This study aimed to determine the prevalence of Campylobacter in wild game pheasants in Scotland, to identify the main sequence types (STs) present and to evaluate their impact on public health...
September 2018: Food Microbiology
https://www.readbyqxmd.com/read/29702315/enteroaggregative-escherichia-coli-is-the-predominant-diarrheagenic-e-coli-pathotype-among-irrigation-water-and-food-sources-in-south-africa
#13
Matthew Aijuka, Araceli E Santiago, Jorge A Girón, James P Nataro, Elna M Buys
Diarrheagenic E. coli (DEC) has been implicated in foodborne outbreaks worldwide and have been associated with childhood stunting in the absence of diarrhoea. Infection is extraordinarily common, but the routes of transmission have not been determined. Therefore, determining the most prevalent pathotypes in food and environmental sources may help provide better guidance to various stakeholders in ensuring food safety and public health and advancing understanding of the epidemiology of enteric disease. We characterized 205 E...
April 20, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29678409/phototherapy-for-neonatal-hyperbilirubinemia-and-childhood-eczema-rhinitis-and-wheeze
#14
Elizabeth Huiwen Tham, Evelyn Xiu Ling Loo, Anne Goh, Oon Hoe Teoh, Fabian Yap, Kok Hian Tan, Keith M Godfrey, Hugo Van Bever, Bee Wah Lee, Yap Seng Chong, Lynette Pei-Chi Shek
BACKGROUND: The pathogenesis of allergic diseases in childhood may be attributed to influences of early environmental stimuli on fetal and neonatal immune regulation. Neonatal hyperbilirubinemia is common in the Asian population and up to 20% of infants require phototherapy. We examined the hypothesis that phototherapy for neonatal hyperbilirubinemia modulates the infant's risk of developing eczema, rhinitis and wheeze in the Growing Up in Singapore Towards healthy Outcomes (GUSTO) birth cohort...
March 24, 2018: Pediatrics and Neonatology
https://www.readbyqxmd.com/read/29666286/viable-but-nonculturable-listeria-monocytogenes-and-salmonella-enterica-serovar-thompson-induced-by-chlorine-stress-remain-infectious
#15
Callum J Highmore, Jennifer C Warner, Steve D Rothwell, Sandra A Wilks, C William Keevil
The microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-tagged Listeria monocytogenes and Salmonella enterica serovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29663680/knowledge-gaps-in-control-of-campylobacter-for-prevention-of-campylobacteriosis
#16
I Hansson, M Sandberg, I Habib, R Lowman, E O Engvall
Campylobacteriosis is an important, worldwide public health problem with numerous socio-economic impacts. Since 2015, approximately 230,000 cases have been reported annually in Europe. In the United States, Australia and New Zealand, campylobacteriosis is the most commonly reported disease. Poultry and poultry products are considered important sources of human infections. Poultry meat can become contaminated with Campylobacter during slaughter if live chickens are intestinal carriers. Campylobacter spp. can be transferred from animals to humans through consumption and handling of contaminated food products, with fresh chicken meat being the most commonly implicated food type...
April 16, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29657395/prevalence-of-liver-flukes-infections-and-hydatidosis-in-slaughtered-sheep-and-goats-in-nishapour-khorasan-razavi-iran
#17
Majid Aminzare, Mohammad Hashemi, Samaneh Yaghoobi Faz, Mojtaba Raeisi, Hassan Hassanzadazar
Background: Food-borne trematode infections and hydatidosis are endemic diseases caused by helminths in Iran that are responsible for great economic loss and getting public health at risk. Aim: Aim of this study was to determine the prevalence of fasciolosis, dicrocoeliasis, and hydatidosis infections in slaughtered sheep and goats in Nishapour, Khorasan Razavi province of Iran. Materials and Methods: A survey was implemented on 130,107 sheep and goats slaughtered at an abattoir in Nishapour (Neyshbur) city, north central Khorasan Razavi Province, Iran, to determine the prevalence of fascioliasis, dicrocoeliosis and presence of hydatidosis...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29652951/-characterization-of-campylobacter-jejuni-samples-coming-form-poultry-meat-and-feces
#18
Sindy Gutiérrez, Daniel Orellana, Claudio Martínez, Verónica García Mena
Background Campylobacter jejuni is one of the main causal agents of food borne diseases. Infections with this pathogen are mainly caused by chicken meat consumption. Aim To characterize antibiotic resistance and virulence factors in C. jejuni strains obtained from chicken meat and poultry feces in Central Chile. Material and Methods The presence of C. jejuni in 30 meat and 40 feces samples from poultry was studied. From these samples, we obtained 40 strains which were characterized at the molecular level for the presence of 16 genes involved in virulence using PCR...
December 2017: Revista Médica de Chile
https://www.readbyqxmd.com/read/29649735/a-review-of-analytical-procedures-for-the-simultaneous-determination-of-medically-important-veterinary-antibiotics-in-environmental-water-sample-preparation-liquid-chromatography-and-mass-spectrometry
#19
REVIEW
Chansik Kim, Hong-Duck Ryu, Eu Gene Chung, Yongseok Kim, Jae-Kwan Lee
Medically important (MI) antibiotics are defined by the United States Food and Drug Administration as drugs containing certain active antimicrobial ingredients that are used for the treatment of human diseases or enteric pathogens causing food-borne diseases. The presence of MI antibiotic residues in environmental water is a major concern for both aquatic ecosystems and public health, particularly because of their potential to contribute to the development of antimicrobial-resistant microorganisms. In this article, we present a review of global trends in the sales of veterinary MI antibiotics and the analytical methodologies used for the simultaneous determination of antibiotic residues in environmental water...
April 9, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29643197/whole-genome-sequencing-of-recent-listeria-monocytogenes-isolates-from-germany-reveals-population-structure-and-disease-clusters
#20
Sven Halbedel, Rita Prager, Stephan Fuchs, Eva Trost, Guido Werner, Antje Flieger
Listeria monocytogenes causes food-borne outbreaks with high mortality. For improvement of outbreak cluster detection, the German consiliary laboratory for listeriosis implemented whole genome sequencing (WGS) in 2015. 424 human L. monocytogenes isolates collected in 2007-2017 were subjected to WGS and core genome multi locus sequence typing (cgMLST). cgMLST grouped the isolates into 38 complexes, reflecting four known and 34 unknown disease clusters. Most of these complexes were confirmed by single nucleotide polymorphism (SNP) calling but some further differentiated...
April 11, 2018: Journal of Clinical Microbiology
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