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Hemolysis

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https://www.readbyqxmd.com/read/28727478/alloimmune-hemolysis-due-to-major-rhe-incompatibility-after-unrelated-cord-blood-transplantation
#1
Masamichi Isobe, Takaaki Konuma, Yuka Abe-Wada, Kyoko Hirata, Kazuo Ogami, Seiko Kato, Maki Oiwa-Monna, Susumu Tanoue, Tokiko Nagamura-Inoue, Satoshi Takahashi, Arinobu Tojo
No abstract text is available yet for this article.
July 20, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28722142/lactic-dehydrogenase-in-the-in-vitro-evaluation-of-hemolytic-properties-of-ventricular-assist-device
#2
Donghai Li, Qingyu Wu, Shuhong Liu, Yuanyuan Chen, Haosheng Chen, Yingmao Ruan, Yu Zhang
Ventricular assist devices (VADs) can effectively improve the survival rate of patients with end-stage heart failure, but the hemolytic complications induced by long-time VAD support have received wide attention recently. The conventional evaluation method of the hemolytic properties of VADs by the indicator of plasma free hemoglobin (PFH) concentration is used but not sensitive enough to meet the needs of the actual examinations. In this study, an experimental method was applied for the evaluation of the injuries and damages caused by VADs to erythrocytes by both indicators of PFH and lactic dehydrogenase (LDH) in the in vitro hemolysis assay of VADs...
July 19, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28722138/a-red-blood-cell-model-to-estimate-the-hemolysis-fingerprint-of-cardiovascular-devices
#3
Riccardo Toninato, Giuseppe Fadda, Francesca Maria Susin
One of the most relevant and open issues within cardiovascular prosthetic hemodynamic performance is a realistic quantification of the damage sustained by red blood cells (RBCs). Specifically, the optimal design of bileaflet mechanical heart valves (BMHVs) requires both low shear stresses along the leaflets and short particle resident times. This study approaches RBC damage estimation by developing a numerical model of RBCs and computing the damage sustained by a set of passive RBCs immersed within in vitro flows...
July 19, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28721816/plga-nanoparticles-and-polysorbate-80-coated-plga-nanoparticles-increase-in-the-vitro-antioxiant-activity-of-melatonin
#4
Leiziani Gnatkowski Martins, Najeh Maissar Khalil, Rubiana Mara Mainardes
Melatonin is an endogen substance with several physiological functions, acting as an important antioxidant. Our objective was to prepare nanoparticles composed of poly(lactic-co-glycolic acid) (PLGA) coated with polysorbate 80 (PLGA-PS80) or uncoated (PLGA) nanoparticles containing melatonin (MLT) and evaluate their in vitro cytotoxicity over erythrocytes and in vitro antioxidant activity. Nanoparticles were obtained by an emulsion-solvent evaporation method and characterized by size, morphology, polydispersity index, zeta potential, encapsulation efficiency, thermal properties and in vitro drug release profile...
July 19, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/28721813/mechanisms-involved-in-the-increased-hemolysis-in-the-fetus-and-newborn
#5
Giuseppe Buonocore, Serafina Perrone, Rodolfo Bracci
BACKGROUND: The suicidal death of erythrocytes should be considered a possible cause of hemolysis and plasma bilirubin overload when there is no evidence of an immune-mediated hemolytic anemia, no consumptive red blood cell disorder, no morphologic or laboratory data to suggest a problem of the red cell membrane, and no evidence of a quantitative or qualitative defect in hemoglobin synthesis. OBJECTIVE: In neonatal period, xenobiotics, cytokines, osmotic shock, energy depletion, oxidative stress, and variation of temperature may induce an alteration of balance between damaging and protecting factors which can be followed by red cell death...
July 18, 2017: Current Pediatric Reviews
https://www.readbyqxmd.com/read/28721663/influence-of-direct-or-indirect-contact-for-the-cytotoxicity-and-blood-compatibility-of-spider-silk
#6
J W Kuhbier, V Coger, J Mueller, C Liebsch, F Schlottmann, V Bucan, P M Vogt, S Strauss
Spider silk became one of the most-researched biomaterials in the last years due to its unique mechanical strength and most favourable chemical composition for tissue engineering purposes. However, standardized analysis of cytocompatibility is missing. Therefore, the aim of this study was to investigate hemolysis, cytotoxicity of native spider silk as well as influences on the cell culture medium. Changes of cell culture medium composition, osmolarity as well as glucose and lactate content were determined via ELISA measurement...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28717776/a-retrospective-study-of-emergency-department-potassium-disturbances-severity-treatment-and-outcomes
#7
Adam J Singer, Henry C Thode, W Frank Peacock
OBJECTIVE: Disturbances in potassium (K) levels are relatively common and may be associated with significant morbidity and mortality; however, treatments vary. Our purpose was to determine the incidence, treatments, and outcomes associated with hyperkalemia and hypokalemia in emergency department (ED) patients. METHODS: We performed a structured, retrospective review of electronic medical records of consecutive adult ED patients with K measured while in the ED. Demographic, clinical, and laboratory data as well as treatments, disposition, and in-hospital complications were collected...
June 2017: Clinical and Experimental Emergency Medicine
https://www.readbyqxmd.com/read/28716864/alpha1-antitrypsin-binds-hemin-and-prevents-oxidative-activation-of-human-neutrophils-putative-pathophysiological-significance
#8
Sabina Janciauskiene, Srinu Tumpara, Malgorzata Wiese, Sabine Wrenger, Vijith Vijayan, Faikah Gueler, Rongjun Chen, Kukuh Madyaningrana, Ravi Mahadeva, Tobias Welte, Stephan Immenschuh, Joanna Chorostowska-Wynimko
Heme is a ubiquitous compound of human tissues, and it is involved in cellular physiology and metabolism. Once released from the cell, free heme oxidizes to the ferric state (hemin). High levels of hemin can cause oxidative stress and inflammation if not neutralized immediately by specialized scavenger proteins. Human alpha1-antitrypsin (A1AT), an acute-phase glycoprotein and important inhibitor of neutrophil proteases, is also a hemin-binding protein. A short-term exposure of freshly isolated human blood neutrophils to 4 µM hemin results in cell spreading, surface expression of filament protein, vimentin, free radical production, expression of heme oxygenase-1 (HO-1), release of IL-8, and enhanced neutrophil adhesion to human endothelial cells...
July 17, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28714216/contributions-of-the-oligopeptide-permeases-in-multistep-of-vibrio-alginolyticus-pathogenesis
#9
Wenjia Liu, Lixing Huang, Yongquan Su, Yingxue Qin, Lingmin Zhao, Qingpi Yan
Vibrio alginolyticus has been associated with several diseases of cultivated marine animals, and has led to considerable economic losses. The oligopeptide permease (Opp) has been proven to play a variety of important roles in nutrition and virulence in several bacteria. In our previous research, the opp gene cluster was identified in Vibrio alginolyticus with transcriptome sequence, which also indicated that the Opp system might play roles in the regulation of adhesion. In this study, the relationship between V...
July 17, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28712866/cell-free-methemoglobin-drives-platelets-to-apoptosis-via-mitochondrial-ros-mediated-activation-of-jnk-and-p38-map-kinase
#10
Somanathapura K NaveenKumar, Mahadevappa Hemshekhar, Mahalingam S Sundaram, Kempaiah Kemparaju, Kesturu S Girish
Cell-free hemoglobin (Hb), a well-known marker of intravascular hemolysis, is eventually oxidized to methemoglobin (MtHb). Elevated levels of MtHb have been noted, alongside depleted levels of platelets, in several hemolytic diseases. The current study aims to probe the possible role of MtHb in platelet death, based on the facts that it is a pro-inflammatory and pro-apoptotic agent, as well as the sensitive nature of platelets and their tendency to undergo apoptosis under oxidative stress. Consequently an attempt is made to establish the link between hemolysis and thrombocytopenia, by deciphering the underlying molecular signaling pathways...
July 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28711436/a-simple-method-for-rapid-microbial-identification-from-positive-monomicrobial-blood-culture-bottles-through-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Jung-Fu Lin, Mao-Cheng Ge, Tsui-Ping Liu, Shih-Cheng Chang, Jang-Jih Lu
BACKGROUND AND PURPOSE: Rapid identification of microbes in the bloodstream is crucial in managing septicemia because of its high disease severity, and direct identification from positive blood culture bottles through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can shorten the turnaround time. Therefore, we developed a simple method for rapid microbiological identification from positive blood cultures by using MALDI-TOF MS. METHODS: We modified previously developed methods to propose a faster, simpler and more economical method, which includes centrifugation and hemolysis...
June 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28711333/b12-deficiency-leading-to-marked-poikilocytosis-versus-true-schistocytosis-a-pernicious-problem
#12
James A Hall, James Mason, Julia Choi, Mark Holguin
Severe vitamin B12 deficiency is caused most commonly by autoimmune atrophic gastritis leading to loss of intrinsic factor. Vitamin B12 deficiency leading to megaloblastic anemia and demyelinating central nervous system disease is well known; however, a rare presentation of B12 deficiency described as pseudothrombotic microangiopathy is not well known. This complication presents with signs of mechanical hemolysis, elevated lactate dehydrogenase (LDH), thrombocytopenia, and a low reticulocyte count, which can be incorrectly diagnosed as thrombotic thrombocytopenic purpura and managed incorrectly...
June 20, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28711077/acute-kidney-injury-in-pregnancy
#13
Belinda Jim, Vesna D Garovic
Pregnancy-related acute kidney injury (AKI) has declined in incidence in the last three decades, although it remains an important cause of maternal and fetal morbidity and mortality. Pregnancy-related causes of AKI such as preeclampsia, acute fatty liver of pregnancy, HELLP (Hemolysis, Elevated Liver function tests, Low Platelets) syndrome, and the thrombotic microangiopathies (thrombotic thrombocytopenic purpura, atypical hemolytic-uremic syndrome [HUS]) exhibit overlapping features and often present as diagnostic dilemmas...
July 2017: Seminars in Nephrology
https://www.readbyqxmd.com/read/28711073/lupus-nephritis-and-pregnancy-concerns-and-management
#14
Liz Lightstone, Michelle A Hladunewich
Pregnancy associated with lupus, especially lupus nephritis, is often fraught with concern for both the mother and fetus. Thus, it is paramount that care begins preconception so that proper planning in terms of optimizing the medical regimen, discontinuation of fetotoxic agents, and treatment of active disease can occur. It is well known that active nephritis at the time of conception is associated with poor outcomes. Even with quiescent disease, recent data indicate that being lupus anticoagulant-positive, nonwhite or Hispanic, and using antihypertensive medications were all predictors of worse pregnancy outcomes...
July 2017: Seminars in Nephrology
https://www.readbyqxmd.com/read/28705502/synthesis-and-characterization-of-hydrogel-films-of-carboxymethyl-tamarind-gum-using-citric-acid
#15
Kailas K Mali, Shashikant C Dhawale, Remeth J Dias
The objective of this study was to synthesize and characterize citric acid crosslinked carboxymethyl tamarind gum (CMTG) hydrogels films. The hydrogel films were characterized by Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, solid state (13)C-nuclear magnetic resonance ((13)C NMR) spectroscopy and differential scanning calorimeter (DSC). The prepared hydrogel films were evaluated for the carboxyl content and swelling ratio. The model drug moxifloxacin hydrochloride was loaded into hydrogels films and drug release was studied at pH 7...
July 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28704937/attenuation-of-red-blood-cell-storage-lesions-with-vitamin-c
#16
Kimberly Sanford, Bernard J Fisher, Evan Fowler, Alpha A Fowler, Ramesh Natarajan
Stored red blood cells (RBCs) undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as "storage lesions". We hypothesized that vitamin C (VitC, reduced or oxidized) would reduce red cell storage lesions, thus prolonging their storage duration. Whole-blood-derived, leuko-reduced, SAGM (saline-adenine-glucose-mannitol)-preserved RBC concentrates were equally divided into four pediatric storage bags and the following additions made: (1) saline (saline); (2) 0...
July 12, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28702563/remarkable-differences-in-the-biochemical-fate-of-cd-2-hg-2-ch3hg-and-thimerosal-in-red-blood-cell-lysate
#17
Matthew A Gibson, Sophia Sarpong-Kumankomah, Susan Nehzati, Graham N George, Jürgen Gailer
Humans are environmentally exposed to potentially toxic Cd and Hg species and to the Hg compound thimerosal (THI), an antibactericidal vaccine additive. Previous studies have revealed that Cd(2+), Hg(2+) and CH3Hg(+) are taken up by red blood cells (RBCs) and bind to cytosolic glutathione (GSH) and/or hemoglobin (Hb). Since interactions in the cytosol of RBCs may be linked to their hemolysis, a more comprehensive characterization of these interactions was sought. After the addition of each Cd and Hg species to RBC lysate, the mixtures were analyzed after 5 min, 2 h and 6 h by size-exclusion chromatography (SEC) coupled on-line to an inductively coupled plasma atomic emission spectrometer (ICP-AES)...
July 12, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28701845/fatal-postoperative-hemolysis-due-to-severe-falciparum-malaria
#18
Amol T Kothekar, Vijaya Patil, Nambiraj Konar, Jigeeshu Divatia
A 60-year-old apparently healthy female patient underwent mastectomy for breast cancer. She had sinus tachycardia and no other abnormal finding in the preoperative period. However, the immediate postoperative course was stormy with the development of anemia, thrombocytopenia, hemolysis, and renal failure with severe metabolic acidosis. Peripheral blood smear revealed the presence of ring forms of Plasmodium falciparum. Multiorgan failure and death occurred within 36 h of surgery in spite of initiation of antimalarial agents...
June 2017: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28701754/clostridium-perfringens-%C3%AE-toxin-impairs-erythropoiesis-by-inhibition-of-erythroid-differentiation
#19
Teruhisa Takagishi, Masaya Takehara, Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi, Masahiro Nagahama
Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119(+) erythroblasts were greatly decreased by the treatment. A variant α-toxin defective in enzymatic activities, phospholipase C and sphingomyelinase, had no effect on the population of erythroblasts, demonstrating that the decrease in erythroblasts was dependent of its enzymatic activities...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28700523/dried-blood-spots-combined-with-ultra-high-performance-liquid-chromatography-mass-spectrometry-for-the-quantification-of-the-antipsychotics-risperidone-aripiprazole-pipamperone-and-their-major-metabolites
#20
Camille Tron, Sanne M Kloosterboer, Bart C H van der Nagel, Rixt A Wijma, Bram Dierckx, Gwen C Dieleman, Teun van Gelder, Birgit C P Koch
BACKGROUND: Risperidone, aripiprazole, and pipamperone are antipsychotic drugs frequently prescribed for the treatment of comorbid behavioral problems in children with autism spectrum disorders. Therapeutic drug monitoring (TDM) could be useful to decrease side effects and to improve patient outcome. Dried blood spot (DBS) sample collection seems to be an attractive technique to develop TDM of these drugs in a pediatric population. The aim of this work was to develop and validate a DBS assay suitable for TDM and home sampling...
August 2017: Therapeutic Drug Monitoring
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