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https://www.readbyqxmd.com/read/29146531/specific-oxygenation-of-plasma-membrane-phospholipids-by-pseudomonas-aeruginosa-lipoxygenase-induces-structural-and-functional-alterations-in-mammalian-cells
#1
Maceler Aldrovandi, Swathi Banthiya, Sven Meckelmann, You Zhou, Dagmar Heydeck, Valerie B O'Donnell, Hartmut Kuhn
Pseudomonas aeruginosa is a gram-negative pathogen, which causes life-threatening infections in immunocompromized patients. These bacteria express a secreted lipoxygenase (PA-LOX), which oxygenates free arachidonic acid to 15S-hydro(pero)xyeicosatetraenoic acid. It binds phospholipids at its active site and physically interacts with lipid vesicles. When incubated with red blood cells membrane lipids are oxidized and hemolysis is induced but the structures of the oxygenated membrane lipids have not been determined...
November 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29145790/innate-immunity-in-cardiac-myxomas-and-its-pathological-and-clinical-correlations
#2
Anna Di Vito, Gianluca Santise, Chiara Mignogna, Eusebio Chiefari, Gabriella Cardillo, Ivan Presta, Franco Arturi, Natalia Malara, Francesco Brunetti, Annalidia Donato, Domenico Maisano, Stefania Leonetti, Daniele Maselli, Tullio Barni, Giuseppe Donato
Cardiac myxomas are the most common benign cardiac tumor. We investigated the immunohistochemical properties of 11 surgically excised cardiac myxomas, in order to analyze the correlation between macrophages and mast cell populations and clinical parameters. CD68(+)/CD163(-)/iNOS(-) (M0) cells represent the most abundant macrophage phenotype; however, CD68(+)/CD163(+) cells (M2) were also frequent. CD68(+)/iNOS(+) (M1) elements were rare. Mast cells, defined as a population of c-kit (CD117)(+) and/or tryptase(+) cells were also detected...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/29145233/prospective-evaluation-of-the-feasibility-safety-and-efficacy-of-cocoon-duct-occluder-for-transcatheter-closure-of-large-patent-ductus-arteriosus-a-single-center-study-with-short-and-medium-term-follow-up-results
#3
Santosh Kumar Sinha, Mahmadula Razi, Rama Niwas Pandey, Prakash Kumar, Vinay Krishna, Mukesh Jitendra Jha, Vikas Mishra, Mohammad Asif, Nasar Abdali, Pradyot Tewari, Ramesh Thakur, Umeshwar Pandey, Chandra Mohan Varma
OBJECTIVE: To evaluate the feasibility, safety, and efficacy of a novel Cocoon Duct Occluder device for the transcatheter closure (TCC) of large patent ductus arteriosus (PDA). METHODS: In this prospective, non-randomized study, consecutive patients with large PDA (narrowest diameter: ≥3.5/4.0 mm in symptomatic/asymptomatic patients, respectively), who underwent TCC with Cocoon Duct Occluder at our institute between November, 2012 and June, 2016 were examined...
November 2017: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/29145096/sulfaguanidine-cocrystals-synthesis-structural-characterization-and-their-antibacterial-and-hemolytic-analysis
#4
Syed Sibte Asghar Abidi, Yasser Azim, Shahper Nazeer Khan, Asad U Khan
Sulfaguanidine (SG), belongs to the class of sulfonamide drug used as an effective antibiotic. In the present work, using crystal engineering approach two novel cocrystals of SG were synthesized (SG-TBA and SG-PT) with thiobarbutaric acid (TBA) and 1,10-phenanthroline (PT), characterized by solid state techniques viz., powder X-ray diffraction (PXRD), fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and the crystal structures were determined by single crystal X-ray diffraction studies...
November 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29141719/chloroquine-primaquine-versus-chloroquine-alone-to-treat-vivax-malaria-in-afghanistan-an-open-randomized-superiority-trial
#5
Ghulam Rahim Awab, Mallika Imwong, Germana Bancone, Atthanee Jeeyapant, Nicholas P J Day, Nicholas J White, Charles J Woodrow
Afghanistan's national guidelines recommend primaquine (PQ) for radical treatment of Plasmodium vivax malaria, but this is rarely implemented because of concerns over potential hemolysis in patients who have G6PD deficiency. Between August 2009 and February 2014, we conducted an open-label, randomized controlled trial of chloroquine (CQ) alone versus chloroquine plus primaquine (0.25 mg base/kg/day for 14 days) (CQ+PQ) in patients aged 6 months and older with microscopy confirmed P. vivax infection. In the CQ+PQ group, G6PD deficiency was excluded by fluorescent spot testing...
October 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29138283/frequency-of-and-reasons-for-paroxysmal-nocturnal-haemoglobinuria-screening-in-patients-with-unexplained-anaemia
#6
James T England, Bakul Dalal, Heather A Leitch
Referral to hematology for anemia is common. In paroxysmal nocturnal hemoglobinuria (PNH), cells deficient in the glycosylphosphatidyl inositol (GPI) anchor are lysed by complement. Eculizumab improves overall survival and quality of life while reducing hemolysis, transfusion requirements, and thrombosis. We evaluated the frequency of screening for PNH in patients with unexplained anemia. Key clinical features, laboratory data, and investigations were recorded for patients referred for anemia since 2010, without a specific cause found...
November 14, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29136469/novel-linear-lipopeptide-paenipeptins-with-potential-for-eradicating-biofilms-and-sensitizing-gram-negative-bacteria-to-rifampicin-and-clarithromycin
#7
Sun Hee Moon, Xuan Zhang, Guangrong Zheng, Daniel G Meeker, Mark S Smeltzer, En Huang
We report the structure-activity relationship (SAR) analyses of 17 linear lipopeptide paenipeptin analogues. Analogues 7, 12 and 17 were more potent than the lead compound. Analogue 17 was active against carbapenem-resistant and polymyxin-resistant pathogens. This compound at 40 μg/mL resulted in 3 log and 2.6 log reductions of methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa, respectively, in catheter-associated biofilms in vitro. Analogue 17 showed little hemolysis at 32 µg/ml and lysed 11% of red blood cells at 64 µg/ml...
November 14, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29133160/the-changes-of-red-blood-cell-viscoelasticity-and-sports-anemia-in-male-24-hr-ultra-marathoners
#8
Che-Hung Liu, Yen-Fang Tseng, Jiun-I Lai, Yin-Quan Chen, Shih-Hao Wang, Wei-Fong Kao, Li-Hua Li, Yu-Hui Chiu, Chorng-Kuang How, Wen-Han Chang
BACKGROUND: In endurance sports, stress, dehydration and release of chemical factors have been associated with red blood cell (RBC) alterations of structure and function, which may contribute to sports anemia, a well-observed phenomenon during long-distance running. Until now, the investigation of the changes of viscoelastic properties of RBC membrane, a decisive factor of RBC deformability to avoid hemolysis, is lacking, especially in an Oriental population. METHODS: nineteen runners were prospectively recruited into our study...
November 10, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29132874/combining-serum-microrna-and-ca-125-as-prognostic-indicators-of-preoperative-surgical-outcome-in-women-with-high-grade-serous-ovarian-cancer
#9
Jaynish S Shah, Gregory B Gard, Jean Yang, Jayne Maidens, Susan Valmadre, Patsy S Soon, Deborah J Marsh
OBJECTIVES: The most widely used approach for the clinical management of women with high-grade serous ovarian cancer (HGSOC) is surgery, followed by platinum and taxane based chemotherapy. The degree of macroscopic disease remaining at the conclusion of surgery is a key prognostic factor determining progression free and overall survival. We sought to develop a non-invasive test to assist surgeons to determine the likelihood of achieving complete surgical resection. This knowledge could be used to plan surgical approaches for optimal clinical management...
November 10, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/29132419/success-of-anti-cd20-monoclonal-antibody-treatment-for-severe-autoimmune-hemolytic-anemia-caused-by-warm-reactive-immunoglobulin-a-immunoglobulin-g-and-immunoglobulin-m-autoantibodies-in-a-child-a-case-report
#10
Houda Ajmi, Sameh Mabrouk, Saida Hassayoun, Haifa Regaieg, Minyar Tfifha, Chemli Jalel, Hadef Skouri, Noura Zouari, Saoussan Abroug
BACKGROUND: Autoimmune hemolytic anemia is rare in children. First-line therapies for this disease consist of corticosteroids and intravenously administered immunoglobulin that are effective in most patients. However, a small proportion of cases (5 to 10%) is refractory to these therapies and may represent a medical emergency, especially when hemolysis is due to warm immunoglobulin M. Recently, reports of the use of rituximab in adult autoimmune diseases have shown promising results. In children, there are few studies on the use of rituximab in the treatment for autoimmune hemolytic anemia, especially on its long-term efficacy and adverse effects...
November 14, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/29129572/biocompatible-fluorinated-poly-%C3%AE-amino-ester-s-for-safe-and-efficient-gene-therapy
#11
Jia-Hui Gong, Yi Wang, Lei Xing, Peng-Fei Cui, Jian-Bin Qiao, Yu-Jing He, Hu-Lin Jiang
Cationic polymers have been widely used as one of the most promising non-viral vehicles for gene delivery due to their potential safety and ease of large-scale production. Here, we report the design and synthesis of a series of novel biodegradable fluorinated poly(β-amino ester)s (FPBAEs) by simple Michael-addition reaction as safe and efficient gene carrier. The results of transfection efficacy assay demonstrated the optimal FPBAE could mediated much higher GFP expression than the commercial transfection agents, polyethyleneimine (PEI, Mw = 25K) and Lipo 2000, as well as the non-fluorinated poly(β-amino ester)s (PBAE) on both HeLa and HEK-293T cell lines (higher than 70 and 90%, respectively), which was largely attributed to fluorination...
November 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29128832/delivery-of-paclitaxel-using-nanoparticles-composed-of-poly-ethylene-oxide-b-poly-butylene-oxide-peo-pbo
#12
Lijiang Wang, Ju Yao, Xiaomin Zhang, Yingxin Zhang, Chang Xu, Robert J Lee, Gary Yu, Bo Yu, Lesheng Teng
An amphiphilic block copolymer poly(ethylene oxide)-b-poly(butylene oxide) (PEO-PBO) was evaluated as a carrier for therapeutic delivery of paclitaxel (PTX). PEO-PBO and PTX form nanoparticles (NPs) by self-assembly upon hydration. The size of these NPs was about 92.71nm and the zeta potential was -5.06mV, which met the requirements for passive tumor targeting through the enhanced permeability and retention effect. Compared with a commonly used block copolymer poly(ethylene glycol)-b-poly-D,L-(lactic acid) (PEG-PDLLA), PEO-PBO forms nanoparticles with superior pharmacokinetic, biodistribution, and tumor inhibitory properties...
November 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29128605/erythrocytes-as-a-biological-model-for-screening-of-xenobiotics-toxicity
#13
REVIEW
Mayada Ragab Farag, Mahmoud Alagawany
Erythrocytes are the main cells in circulation. They are devoid of internal membrane structures and easy to be isolated and handled providing a good model for different assays. Red blood cells (RBCs) plasma membrane is a multi-component structure that keeps the cell morphology, elasticity, flexibility and deformability. Alteration of membrane structure upon exposure to xenobiotics could induce various cellular abnormalities and releasing of intracellular components. Therefore the morphological changes and extracellular release of haemoglobin [hemolysis] and increased content of extracellular adenosine triphosphate (ATP) [as signs of membrane stability] could be used to evaluate the cytotoxic effects of various molecules...
November 8, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29128593/nanochitosan-enriched-poly-%C3%AE%C2%B5-caprolactone-electrospun-wound-dressing-membranes-a-fine-tuning-of-physicochemical-properties-hemocompatibility-and-curcumin-release-profile
#14
Reshmi Cr, Suja Ps, Manaf O, Sanu Pp, Sujith A
Nanochitosan (NC) enriched Poly (ε-caprolactone) (PCL) nanofibrous membranes were fabricated for controlled curcumin delivery and wound dressing. A significant increase in hydrophilicity, PBS sorption, and vapour transmission were observed by incorporation of NC in PCL. Hemolysis assay, blood clot kinetics and platelet adhesion studies on PCL/NC membrane confirmed the hemocompatibility of the scaffold. NC also imparts appropriate antibacterial activity and biocompatibility promising for a wound dressing material...
November 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29128072/paroxysmal-nocturnal-hemoglobinuria-assessment-by-flow-cytometric-analysis
#15
REVIEW
Mike Keeney, Andrea Illingworth, D Robert Sutherland
Paroxysmal nocturnal hemoglobinuria (PNH) is an uncommon but frequently debilitating disease that, if untreated, may lead to death in up to 35% of patients within 5 years. Assessment of PNH clone size by flow cytometric analysis has increased in importance with the availability of therapeutic treatments, which prevent the hemolysis of red blood cells and, hence, the myriad symptoms that accompany the disease. This article addresses flow cytometric methodologies and highlights areas of importance in implementing testing, not only for classic PNH but also for other related bone marrow failure disorders, such as aplastic anemia and low-grade myelodysplastic syndrome...
December 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/29127281/structural-and-functional-insight-of-sphingosine-1-phosphate-mediated-pathogenic-metabolic-reprogramming-in-sickle-cell-disease
#16
Kaiqi Sun, Angelo D'Alessandro, Mostafa H Ahmed, Yujin Zhang, Anren Song, Tzu-Ping Ko, Travis Nemkov, Julie A Reisz, Hongyu Wu, Morayo Adebiyi, Zhangzhe Peng, Jing Gong, Hong Liu, Aji Huang, Yuan Edward Wen, Alexander Q Wen, Vladimir Berka, Mikhail V Bogdanov, Osheiza Abdulmalik, Leng Han, Ah-Lim Tsai, Modupe Idowu, Harinder S Juneja, Rodney E Kellems, William Dowhan, Kirk C Hansen, Martin K Safo, Yang Xia
Elevated sphingosine 1-phosphate (S1P) is detrimental in Sickle Cell Disease (SCD), but the mechanistic basis remains obscure. Here, we report that increased erythrocyte S1P binds to deoxygenated sickle Hb (deoxyHbS), facilitates deoxyHbS anchoring to the membrane, induces release of membrane-bound glycolytic enzymes and in turn switches glucose flux towards glycolysis relative to the pentose phosphate pathway (PPP). Suppressed PPP causes compromised glutathione homeostasis and increased oxidative stress, while enhanced glycolysis induces production of 2,3-bisphosphoglycerate (2,3-BPG) and thus increases deoxyHbS polymerization, sickling, hemolysis and disease progression...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29125279/molecular-weight-and-charge-density-effects-of-guanidinylated-biodegradable-polycarbonates-on-antimicrobial-activity-and-selectivity
#17
Chloe A Hae Cho, Chao Liang, Janesha Perera, Jie Liu, Kyriakos G Varnava, Vijayalekshmi Sarojini, Ralph P Cooney, Duncan J McGillivray, Margaret A Brimble, Simon Swift, Jianyong Jin
Six guanidine functionalized aliphatic biodegradable polycarbonates with varying molecular weights and charge densities were synthesized via post-synthesis modification of alkyne containing polycarbonates using Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. The concept of passive diluting group was to modify the cationic charge density of the polycarbonate without changing its hydrophilicity. Within the molecular weight range from 8,000 g mol-1 to 30,000 g mol-1, these guanidine polycarbonates exhibited broad-spectrum biocidal activity with low toxicity to red blood cells (RBCs)...
November 10, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29124560/maintenance-treatment-using-the-purine-synthesis-inhibitor-mizoribine-in-a-patient-with-relapsing-thrombotic-thrombocytopenic-purpura
#18
Tetsuya Kawamura, Joichi Usui, Kei Nagai, Kentaro Sakai, Itaru Ebihara, Kunihiro Yamagata
Thrombotic thrombocytopenic purpura (TTP) is a rare but life-threatening disease. Although plasma exchange (PE) therapy and corticosteroids are standard remission induction and maintenance therapies, some patients are easily refractory and frequently relapse under treatment with this therapy, and require additional treatment. However, there are limited data about additional treatment interventions. We report a case of 56-year-old man who was hospitalized for fever, general fatigue and hemoglobinuria. Owing to the symptoms and the laboratory findings of hemolysis, he was diagnosed with TTP...
November 9, 2017: CEN Case Reports
https://www.readbyqxmd.com/read/29124521/successful-peritoneal-dialysis-using-a-percutaneous-tube-for-peritoneal-drainage-in-an-extremely-low-birth-weight-infant-a-case-report
#19
Satoshi Yokoyama, Takayuki Nukada, Yuka Ikeda, Shigeto Hara, Akira Yoshida
BACKGROUND: Peritoneal dialysis (PD) for acute kidney injury (AKI) of newborns has been performed safely. AKI occurs in 8 to 24% of extremely low birth weight (ELBW) infants. Although PD has only been used occasionally in ELBW infants, prognosis is poor for ELBW infants with AKI. Several reports have described successful PD in these infants, but no guideline-based evidence concerning indications for renal replacement therapy in ELBW infants are currently available. Here, we report on our experience with PD in an ELBW infant with AKI resulting from septic shock...
November 9, 2017: Surgical Case Reports
https://www.readbyqxmd.com/read/29122736/enhanced-antimalalarial-activity-of-a-prolonged-release-in-situ-gel-of-arteether-lumefantrine-in-a-murine-model
#20
Shilpa Dawre, Sulabha Pathak, Shobhona Sharma, Padma V Devarajan
The World Health Organization (WHO) recommends artemisinin-based combination therapy (ACT) for treatment of falciparum malaria. Arteether (ART), an artemisinin derivative, is effective against Plasmodium falciparum, but it is available only as painful oily intramuscular (i.m.) injections. We formulated lyotropic liquid crystalline preconcentrates of ART and Lumefantrine (LUM) ACT with and without biodegradable polymer for antimalarial therapy. Following i.m. injection, both formed intact gels in situ due to rapid transition into liquid crystalline phase (LCP) which was confirmed by small angle neutron scattering (SANS), x-ray diffraction (XRD), polarization optical microscopy (POM) and rheological changes...
November 6, 2017: European Journal of Pharmaceutics and Biopharmaceutics
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