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https://www.readbyqxmd.com/read/28514689/identification-of-a-human-clonogenic-progenitor-with-strict-monocyte-differentiation-potential-a-counterpart-of-mouse-cmops
#1
Shunsuke Kawamura, Nobuyuki Onai, Fuyuki Miya, Taku Sato, Tatsuhiko Tsunoda, Kazutaka Kurabayashi, Satoshi Yotsumoto, Shoko Kuroda, Katsuto Takenaka, Koichi Akashi, Toshiaki Ohteki
Monocytes give rise to macrophages and dendritic cells (DCs) under steady-state and inflammatory conditions, thereby contributing to host defense and tissue pathology. A common monocyte progenitor (cMoP) that is strictly committed to the monocyte lineage has been recently identified in mice. Here, we identified human cMoPs as a CLEC12A(hi)CD64(hi) subpopulation of conventional granulocyte-monocyte progenitors (cGMPs) in umbilical cord blood and in bone marrow. Human cMoPs gave rise to monocyte subsets without showing any potential for differentiating into myeloid or lymphoid cells...
May 16, 2017: Immunity
https://www.readbyqxmd.com/read/28502306/-detection-and-analysis-of-immune-molecules-in-leukemia-cell-surfaces-of-acute-promyelocytic-leukemia
#2
Ting Dong, Yaxian Jiang, Zhengfa Li, Hongmei Ouyang, Qin Zhang
Objective To investigate the immunophenotypic characteristics of acute promyelocytic leukemia (APL) and its clinical significance. Methods Immunophenotyping was performed by four-color flow cytometry with CD45/SSC-lin gating for neoplastic cells and was divided into five levels according to the intensity of antigen expression. Results The expression intensity and percentage of typical APL phenotypes were: myeloperoxidase (MPO) and CD33 were consistently expressed (100%); CD38 (82.35%), CD13 (64.71%), CD64 (50%), CD123 (47...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28501938/zoledronic-acid-renders-human-m1-and-m2-macrophages-susceptible-to-v%C3%AE-2-%C3%AE-%C3%AE-t-cell-cytotoxicity-in-a-perforin-dependent-manner
#3
Daniel W Fowler, John Copier, Angus G Dalgleish, Mark D Bodman-Smith
Vδ2(+) T cells are a subpopulation of γδ T cells in humans that are cytotoxic towards cells which accumulate isopentenyl pyrophosphate. The nitrogen-containing bisphosphonate, zoledronic acid (ZA), can induce tumour cell lines to accumulate isopentenyl pyrophosphate, thus rendering them more susceptible to Vδ2(+) T cell cytotoxicity. However, little is known about whether ZA renders other, non-malignant cell types susceptible. In this study we focussed on macrophages (Mϕs), as these cells have been shown to take up ZA...
May 13, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28446314/-changes-of-neutrophil-cd64-in-patients-with-hematological-mali-gnancies-combined-with-bacterial-infections
#4
Xin Dai, Jian-Ping Li, Wen-Qian Li, Jian-Ming Feng
OBJECTIVE: To explore the changes of CD64 on surface of neutrophils in patients with hematological malignancies combined with bacterial infections. METHODS: Ninety-seven patients with hematological tumor admitted in our hospital from August 2014 to February 2016 were selected and divided into 2 groups: infection group(50 cases) and noninfection group(47 cases) according to their symptoms, physical sings and blood culture results for microbiologic detection. The CD46 index on surface of neutrophils, serum C- reactive protein (CRP), neutrophil count (NC) and procalcitonin (PCT) level were detected by flow cytometry...
April 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28442521/application-of-flow-cytometry-in-the-early-diagnosis-of-neonatal-sepsis
#5
Mustafa Aydin, Sumeyye Barut, H Handan Akbulut, Sit Ucar, Aysen Orman
GOALS: To raise awareness of the utility of flow cytometric detection of inflammatory markers in the early diagnosis of neonatal sepsis. PROCEDURES: In accordance with the Töllner scoring system, cases with ≥10 points are accepted as having "clinical sepsis" and cases with 0-4 points as having "no sepsis". The study group consisted of 50 newborns with clinical sepsis as well as a control group of 50 newborns without sepsis. In all cases, blood counts, C-reactive protein (CRP) levels, and procalcitonin (PCT) levels were recorded...
March 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28415754/gene-modified-nk-92mi-cells-expressing-a-chimeric-cd16-bb-%C3%AE-or-cd64-bb-%C3%AE-receptor-exhibit-enhanced-cancer-killing-ability-in-combination-with-therapeutic-antibody
#6
Ying Chen, Fengtao You, Licui Jiang, Jialu Li, Xuejun Zhu, Yangyi Bao, Xiang Sun, Xiaowen Tang, Huimin Meng, Gangli An, Bozhen Zhang, Lin Yang
Natural killer (NK) cells play a pivotal role in monoclonal antibody-mediated immunotherapy through the antibody-dependent cell-mediated cytotoxicity (ADCC) mechanism. NK-92MI is an interleukin-2 (IL-2)-independent cell line, which was derived from NK-92 cells with superior cytotoxicity toward a wide range of tumor cells in vitro and in vivo. Nonetheless, the Fc-receptor (CD16) that usually mediates ADCC is absent in NK-92 and NK-92MI cells. To apply NK-92MI cell-based immunotherapy to cancer treatment, we designed and generated two chimeric receptors in NK-92MI cells that can bind the Fc portion of human immunoglobulins...
March 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28400539/mass-cytometry-deep-phenotyping-of-human-mononuclear-phagocytes-and-myeloid-derived-suppressor-cells-from-human-blood-and-bone-marrow
#7
Mikael Roussel, P Brent Ferrell, Allison R Greenplate, Faustine Lhomme, Simon Le Gallou, Kirsten E Diggins, Douglas B Johnson, Jonathan M Irish
The monocyte phagocyte system (MPS) includes numerous monocyte, macrophage, and dendritic cell (DC) populations that are heterogeneous, both phenotypically and functionally. In this study, we sought to characterize those diverse MPS phenotypes with mass cytometry (CyTOF). To identify a deep phenotype of monocytes, macrophages, and DCs, a panel was designed to measure 38 identity, activation, and polarization markers, including CD14, CD16, HLA-DR, CD163, CD206, CD33, CD36, CD32, CD64, CD13, CD11b, CD11c, CD86, and CD274...
April 11, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28396236/skin-inflammation-induced-by-lupus-serum-was-inhibited-in-il-1r-deficient-mice
#8
Xiaoyan Li, Xuanxuan Guo, Huicheng Liu, Gongming Gao, Guangqiong Xu, Xibin Fei, Xiang Fang, Wei Qiao, Guo-Min Deng
Skin inflammation induced by lupus serum is a useful tool to investigate the pathogenesis of lupus skin injury. IL-1 is a proinflammatory cytokine, and its role in lupus skin lesion is still unclear. We determined the role of IL-1 in lupus skin injury by using gene deficient mice. We found that skin inflammation induced by lupus serum was significantly reduced in IL-1R deficient mice and caspase-1 deficient mice. IL-1R deficiency did not affect the expression of FcγRI (CD64), FcγRII (CD32) and MHC class II (CD74) induced by lupus serum...
April 7, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28389358/microrna-125b-inhibits-aml-cells-differentiation-by-directly-targeting-fes
#9
Jinhang Hu, Lufeng Zheng, Xiao Shen, Yan Zhang, Cheng Li, Tao Xi
MicroRNA-125b (miR-125b) has been reported to be upregulated in several kinds of leukemia, suggesting that miR-125b plays a role in Leukemia development. In this study, it was shown that miR-125b expression level decreased in response to 1α, 25-dihydroxy-vitamin D3 (1,25D3) in a dose- and time-dependent manner and miR-125b blocked 1,25D3-induced monocytic differentiation of U937 cells. In addition, miR-125b decreased mRNA expression of myelomonocytic differentiation markers, including CD11c, CD18 and CD64 and arrested the cell cycle at the S phase in U937 and HL60 cells...
April 4, 2017: Gene
https://www.readbyqxmd.com/read/28378496/c-reactive-protein-and-ageing
#10
Ying Tang, Erik Fung, Anping Xu, Hui-Yao Lan
Increasing evidence shows that C-reactive protein (CRP) is not only an inflammatory biomarker but also an important risk factor associated with ageing-related diseases including cardiovascular disease, hypertension, diabetes mellitus, and kidney disease. Recent studies have demonstrated that CRP is pathogenic in a number of diseases including hypertensive cardiovascular and kidney complications, diabetic nephropathy, and acute and chronic kidney diseases. It is well known that CRP binds its receptor, CD32/CD64, to induce the process of inflammation by activating the NF-κB signalling pathway...
April 4, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28332085/low-level-arsenic-causes-chronic-inflammation-and-suppresses-expression-of-phagocytic-receptors
#11
Priyanka Prasad, Dona Sinha
The impact of chronic low-level groundwater arsenic (As) exposure [in the range above the WHO-recommended limit of 10 μg/L but ≤50 μg/L (permissible limit of As for many Asian countries)] was investigated for cross talk of inflammatory changes and expression of phagocytic receptors of exposed rural women (N, 45) from districts of 24 Parganas (south) and in matched control groups (N, 43) [As ≤10 μg/L] from the same district. Systemic inflammation was evident from the upregulated levels of pro-inflammatory mediators like tumor necrosis factor-α (TNF-α); interleukins (ILs) like IL-6, IL-8, and IL-12; and C-reactive protein (CRP) in the sera and upregulated expression of protein kinase B phosphorylated at ser473 (pAKTser473)/nuclear factor-κB (NF-κB)/TNF-α axis in the leukocytes of exposed women with respect to control...
March 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28288151/pentoxifylline-modulates-lps-induced-hyperinflammation-in-monocytes-of-preterm-infants-in-vitro
#12
Simone S Schüller, Lukas Wisgrill, Elisabeth Herndl, Andreas Spittler, Elisabeth Förster-Waldl, Kambis Sadeghi, Boris W Kramer, Angelika Berger
BACKGROUND: Pentoxifylline (PTX), a methylxanthine derivate with immunomodulating properties, has been used as adjunctive treatment in severe neonatal sepsis. The aim of the study was to investigate PTX's anti-inflammatory effects on LPS stimulated monocytes of preterm neonates in vitro compared to monocytes from term infants and adult controls. METHODS: Whole cord blood and control adult blood samples were incubated with LPS and PTX. The expression of surface markers, phagocytosis, cytokine secretion and Toll-like-receptor (TLR)4 signaling of monocytes were assessed by flow cytometry...
March 13, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28264969/cooperation-of-gastric-mononuclear-phagocytes-with-helicobacter-pylori-during-colonization
#13
Monica Viladomiu, Josep Bassaganya-Riera, Nuria Tubau-Juni, Barbara Kronsteiner, Andrew Leber, Casandra W Philipson, Victoria Zoccoli-Rodriguez, Raquel Hontecillas
Helicobacter pylori, the dominant member of the human gastric microbiota, elicits immunoregulatory responses implicated in protective versus pathological outcomes. To evaluate the role of macrophages during infection, we employed a system with a shifted proinflammatory macrophage phenotype by deleting PPARγ in myeloid cells and found a 5- to 10-fold decrease in gastric bacterial loads. Higher levels of colonization in wild-type mice were associated with increased presence of mononuclear phagocytes and in particular with the accumulation of CD11b(+)F4/80(hi)CD64(+)CX3CR1(+) macrophages in the gastric lamina propria...
April 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28257233/three-unique-interstitial-macrophages-in-the-murine-lung-at-steady-state
#14
Sophie L Gibbings, Stacey M Thomas, Shaikh M Atif, Alexandra L McCubbrey, A Nicole Desch, Thomas Danhorn, Sonia M Leach, Donna L Bratton, Peter M Henson, William J Janssen, Claudia V Jakubzick
RATIONALE: The current paradigm in macrophage biology is that some tissues mainly contain macrophages from embryonic origin such as microglia in the brain, while other tissues contain postnatal-derived macrophages, such as the gut. However, in the lung and in other organs such as the skin, there are both embryonic and postnatal-derived macrophages. OBJECTIVES: In this study, we demonstrate in the steady-state lung that the mononuclear phagocyte system is comprised of three newly identified interstitial macrophages (IMs), alveolar macrophages (AMs), dendritic cells (DCs) and few extravascular monocytes...
March 3, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28250006/cebpa-double-mutated-acute-myeloid-leukemia-displays-a-unique-phenotypic-profile-a-reliable-screening-method-and-insight-into-biological-features
#15
Francesco Mannelli, Vanessa Ponziani, Sara Bencini, Maria Ida Bonetti, Matteo Benelli, Ilaria Cutini, Giacomo Gianfaldoni, Barbara Scappini, Fabiana Pancani, Matteo Piccini, Tommaso Rondelli, Roberto Caporale, Anna Maria Grazia Gelli, Benedetta Peruzzi, Marco Chiarini, Erika Borlenghi, Orietta Spinelli, Damiano Giupponi, Pamela Zanghì, Renato Bassan, Alessandro Rambaldi, Giuseppe Rossi, Alberto Bosi
Mutations in CCAAT/enhancer binding protein α (CEBPA) occur in 5-10% of cases of acute myeloid leukemia. CEBPA-double-mutated cases usually bear biallelic N- and C-terminal mutations and are associated with a favorable clinical outcome. Identification of CEBPA mutants is challenging because of the variety of mutations, intrinsic characteristics of the gene and technical issues. Several screening methods (fragment-length analysis, gene expression array) have been proposed especially for large-scale clinical use; although efficient, they are limited by specific concerns...
March 2017: Haematologica
https://www.readbyqxmd.com/read/28245371/-expression-of-cd146-in-adult-and-children-s-acute-b-cell-lymphoblastic-leukemia-and-its-significance
#16
Xiao-Qing Xie, Wei-Min Wang, Si-Lin Gan, Sheng-Mei Chen, Qiu-Tang Zhang, Xin-Sheng Xie, Yan-Fang Liu, Yuan-Dong Cheng, Yu-Feng Liu, Xue-Ju Xu, Hui Sun
OBJECTIVE: To explore the differences of CD146 expression in adult and children's acute B cell lymphoblastic leukemia(B-ALL), and its relation with clinical features, molecular biological and cytogenctic claracteristics. METHODS: The expression of CD146 in bone marrow samples from adult and children's B-ALL patients were detected by flow cytometry (FCM) and the relation of CD146 abnormal high expression with the patients' clinical features, molecular biological and cytogenetical characteristics, as well as other antigens were analyzed...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28213502/monocytes-are-the-predominant-cell-type-associated-with-listeria-monocytogenes-in-the-gut-but-they-do-not-serve-as-an-intracellular-growth-niche
#17
Grant S Jones, Sarah E F D'Orazio
After foodborne transmission of the facultative intracellular bacterial pathogen Listeria monocytogenes, most of the bacterial burden in the gut is extracellular. However, we previously demonstrated that intracellular replication in an as yet unidentified cell type was essential for dissemination and systemic spread of L. monocytogenes In this article, we show that the vast majority of cell-associated L. monocytogenes in the gut were adhered to Ly6C(hi) monocytes, a cell type that inefficiently internalized L...
April 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28208160/quantitative-and-qualitative-profiles-of-circulating-monocytes-may-help-identifying-tuberculosis-infection-and-disease-stages
#18
Marco Pio La Manna, Valentina Orlando, Francesco Dieli, Paola Di Carlo, Antonio Cascio, Gilda Cuzzi, Fabrizio Palmieri, Delia Goletti, Nadia Caccamo
Tuberculosis (TB) is one of the most important cause of morbidity and death among infectious diseases, and continuous efforts are needed to improve diagnostic tools and therapy. Previous published studies showed that the absolute cells number of monocytes or lymphocytes in peripheral blood or yet the ratio of monocytes to lymphocytes displayed the ability to predict the risk of active TB. In the present study we evaluated the ratio of monocytes to lymphocytes variation and we also analyzed the ex-vivo expression of CD64 on monocytes as tools to identify biomarkers for discriminating TB stages...
2017: PloS One
https://www.readbyqxmd.com/read/28203119/higher-phagocytic-activity-of-thioglycollate-elicited-peritoneal-macrophages-is-related-to-metabolic-status-of-the-cells
#19
Sofia Pavlou, Luxi Wang, Heping Xu, Mei Chen
BACKGROUND: Peritoneal macrophages are widely used in immunological studies. The cells can be collected under non-elicited (resident) or elicited (e.g., with Brewer thioglycollate broth injection) conditions, and their phenotype and functions differ. Recent studies have shown that macrophage phenotype and function are related to their metabolic states, and metabolic reprogramming has been an emerging concept for controlling macrophage function. In this study, we examined the metabolic state of resident and elicited macrophages and investigated how their metabolic state may affect cell function, including phagocytosis...
2017: Journal of Inflammation
https://www.readbyqxmd.com/read/28185443/neutrophil-lymphocyte-ratio-better-addition-to-crp-than-cd64-index-as-marker-for-infection-in-copd
#20
Raymond Farah, Rabia Ibrahim, Majd Nassar, Dally Najib, Yifat Zivony, Elizabeth Eshel
OBJECTIVE: This study investigated the importance of neutrophil-lymphocyte ratio (NLR) and other new inflammatory markers including CD64 expression in patients with chronic obstructive pulmonary disease (COPD) for identifying the severity of inflammation and recognition of acute exacerbation and infection. METHODS: 72 patients with a diagnosis of COPD exacerbation who were admitted to Internal Medicine B department, 13 stable COPD, and control group of 15 healthy persons were enrolled in the study...
February 9, 2017: Panminerva Medica
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