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https://www.readbyqxmd.com/read/29156691/neutrophils-in-chronic-lymphocytic-leukemia-are-permanently-activated-and-have-functional-defects
#1
Gayane Manukyan, Tomas Papajik, Petr Gajdos, Zuzana Mikulkova, Renata Urbanova, Gabriela Gabcova, Milos Kudelka, Peter Turcsányi, Pavlina Ryznerova, Vit Prochazka, Eva Kriegova
A growing body of studies highlights involvement of neutrophils in cancer development and progression. Our aim was to assess the phenotypic and functional properties of circulating neutrophils from patients with chronic lymphocytic leukemia (CLL). The percentage of CD54+ and CD64+ neutrophils as well as CD54 expression on these cells were higher in CLL patients than in age-matched healthy controls. Neutrophils from CLL produced more reactive oxygen species (ROS) compared to controls in both resting and activated conditions...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29155023/therapy-related-myeloid-neoplasm-in-an-18-year-old-boy-with-b-lymphoblastic-leukemia
#2
Xin Qing, Eduard Panosyan, ChangjunYue, Ping Ji, Moran Gotesman, Samuel French, Junchao Cai
BACKGROUND: Acute lymphoblastic leukemia (ALL) is the most common pediatric malignancy. Acute myeloid leukemia or myelodysplastic syndrome during the course of ALL is a rare entity. Some of these cases are therapy-related while the others occur due to lineage switch. The correct diagnosis relies on comparing the immunophenotypes and cytogenetic/molecular alterations of the myeloid neoplasm and the ALL. We present the clinical, pathologic and cytogenetic features of a case of an 18-year-old male with prior treatment for B-lymphoblastic leukemia (B-ALL) who developed therapy-related myeloid neoplasm (t-MN) 4-5years after his initial diagnosis of B-ALL...
November 16, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29144515/detection-of-sepsis-in-patient-blood-samples-using-cd64-expression-in-a-microfluidic-cell-separation-device
#3
Ye Zhang, Wenjie Li, Yun Zhou, Amanda Johnson, Amanda Venable, Ahmed Hassan, John Griswold, Dimitri Pappas
A microfluidic affinity separation device was developed for the detection of sepsis in critical care patients. An affinity capture method was developed to capture cells based on changes in CD64 expression in a single, simple microfluidic chip for sepsis detection. Both sepsis patient samples and a laboratory CD64+ expression model were used to validate the microfluidic assay. Flow cytometry analysis showed that the chip cell capture had a linear relationship with CD64 expression in laboratory models. The Sepsis Chip detected an increase in upregulated neutrophil-like cells when the upregulated cell population is as low as 10% of total cells spiked into commercially available aseptic blood samples...
November 16, 2017: Analyst
https://www.readbyqxmd.com/read/29143175/vectors-for-expression-of-signal-peptide-dependent-proteins-in-baculovirus-insect-cell-systems-and-their-application-to-expression-and-purification-of-the-high-affinity-immunoglobulin-gamma-fc-receptor-i-in-complex-with-its-gamma-chain
#4
Le T M Le, Jens R Nyengaard, Monika M Golas, Bjoern Sander
Integral membrane proteins play a central role in various cellular functions and are important therapeutic targets. However, technical challenges in the overexpression and purification of membrane proteins often represent a limiting factor for biochemical and structural studies. Here, we constructed a set of vectors, derivatives of MultiBac vectors that can be used to express proteins with a cleavable N-terminal signal peptide in insect cells. We propose these vectors for expression of type I membrane proteins and other secretory pathway proteins that require the signal recognition particle for translocation to the endoplasmic reticulum (ER)...
November 15, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29138816/protective-effects-of-fucoidan-against-hyperoxic-lung-injury-via-the-erk-signaling-pathway
#5
Minghao Nie, Yan Wang, Yanhong Lu, Ying Yuan, Yingying Liu, Xiurong Li
High oxygen mechanical ventilation is widely used to treat various lung diseases; however, it may result in hyperoxia, which induces inflammation and lung injury. Fucoidan is an extract of the seaweed Fucus vesiculosus, which has previously been reported to exert effects against diabetic nephropathy. The present study is the first, to the best of our knowledge, to investigate the protective effects of fucoidan against hyperoxic lung injury. Balb/c mice were ventilated with 100% oxygen, with or without the atomization inhalation of fucoidan, for 36 h...
November 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29120579/expression-of-cd64-on-surface-of-circulating-monocytes-in-systemic-lupus-erythematosus-patients-relation-to-disease-activity-and-lupus-nephritis
#6
Yasser A Abd-Elhamid, Refaat M Eltanawy, Rasha M Fawzy, Nehad A Fouad, Ann M Atlm
CD64 is a type of integral membrane glycoprotein known as FC receptor that binds monomeric IgG-type antibodies with high affinity. It is more commonly known as FC gamma receptor 1 (FC?R1) and it is expressed on monocytes surface. The goal of this study was to investigate the association of CD64 expression on the surface of peripheral blood monocytes of systemic lupus erythematosus patients with disease activity, and lupus nephritis. 30 SLE patients were enrolled into this study. They were subdivided into: 15 SLE patients with lupus nephritis and 15 SLE patients without lupus nephritis...
January 2017: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/29120575/neutrophil-cd11b-cd64-and-lipocalin-2-early-diagnostic-markers-of-neonatal-sepsis
#7
Abeer Sheneef, Tamer Mohamed, Naglaa F Boraey, Mostafa A Mohammed
Neonatal sepsis remains a global health problem particularly in the developing countries. Its diagnosis remains one of the most difficult issues in clinical medicine. Many immunologic markers including neutrophils CD11b and CD 64 and Lipocalin-2 have been tested as biomarkers of neonatal sepsis. The aim of the present study was to assess the value of these markers for early diagnosis of neonatal sepsis. The study included 60 neonates with suspected neonatal sepsis and 20 apparently healthy controls. Lipocalin-2 serum level was assessed by ELISA while neutrophils CD11b and CD64 expressions were evaluated by flow cytometry...
January 2017: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/29113330/flow-cytometric-analysis-of-cd64-expression-pattern-and-density-in-the-diagnosis-of-acute-promyelocytic-leukemia-a-multi-center-study-in-shanghai-china
#8
Min Liu, Xiangqin Weng, Shenglan Gong, Hui Chen, Jing Ding, Mengqiao Guo, Xiaoxia Hu, Jianmin Wang, Jianmin Yang, Gusheng Tang
No unified immunophenotypic profiles and corresponding analytic strategies have been established for the rapid diagnosis of acute promyelocytic leukemia (APL) using flow cytometry (FCM). Here we describe a characteristic immunophenotypic panel that can rapidly and accurately distinguish APL from other types of adult acute myeloid leukemia (AML) using only FCM. By comparing APL cells and non-APL AML cells that share APL common immunophenotypes (CD34(-)CD117(+)HLA(-)DR(-)) we found that CD64 was a significant factor that differentiated APL from other AMLs...
October 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/29101312/gingival-tissue-inflammation-promotes-increased-matrix-metalloproteinase-12-production-by-cd200r-low-monocyte-derived-cells-in-periodontitis
#9
Sofia Björnfot Holmström, Reuben Clark, Stephanie Zwicker, Daniela Bureik, Egle Kvedaraite, Eric Bernasconi, Anh Thu Nguyen Hoang, Gunnar Johannsen, Benjamin J Marsland, Elisabeth A Boström, Mattias Svensson
Irreversible tissue recession in chronic inflammatory diseases is associated with dysregulated immune activation and production of tissue degradative enzymes. In this study, we identified elevated levels of matrix metalloproteinase (MMP)-12 in gingival tissue of patients with the chronic inflammatory disease periodontitis (PD). The source of MMP12 was cells of monocyte origin as determined by the expression of CD14, CD68, and CD64. These MMP12-producing cells showed reduced surface levels of the coinhibitory molecule CD200R...
November 3, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29084248/tti-621-sirp%C3%AE-fc-a-cd47-blocking-cancer-immunotherapeutic-triggers-phagocytosis-of-lymphoma-cells-by-multiple-polarized-macrophage-subsets
#10
Gloria H Y Lin, Vien Chai, Vivian Lee, Karen Dodge, Tran Truong, Mark Wong, Lisa D Johnson, Emma Linderoth, Xinli Pang, Jeff Winston, Penka S Petrova, Robert A Uger, Natasja N Viller
Tumor-associated macrophages (TAMs) are heterogeneous and can adopt a spectrum of activation states between pro-inflammatory and pro-tumorigenic in response to the microenvironment. We have previously shown that TTI-621, a soluble SIRPαFc fusion protein that blocks the CD47 "do-not-eat" signal, promotes tumor cell phagocytosis by IFN-γ-primed macrophages. To assess the impact of CD47 blockade on diverse types of macrophages that are found within the tumor microenvironment, six different polarized human macrophage subsets (M(-), M(IFN-γ), M(IFN-γ+LPS), M(IL-4), M(HAGG+IL-1β), M(IL-10 + TGFβ)) with distinct cell surface markers and cytokine profiles were generated...
2017: PloS One
https://www.readbyqxmd.com/read/29075958/a-rare-case-of-b-lymphoproliferative-disorder-with-villous-lymphocytes-harboring-t-8-14-q24-q32-translocation
#11
Xiaofeng Shi, Rong Ba, Haiyan You, Qian Jiang, Jiansong Huang, Jianhua Mao, Lanxiu Han, Shuo Zhang, Qin Zhuang, Xianqiu Yu, Lixia Wang, Yun Wang, Dongya Li, Wei Zhu, Yong Zhang, Yan Zhu, Xiaodong Xi
Splenic lymphoma with villous lymphocytes (SLVL) or splenic marginal zone lymphoma with circulating villous lymphocytes is rare, and prolymphocytic transformation of SLVL is rarer. At present, only one case of SLVL with t(8;14)(q24;q32) translocation has been reported. In this study, we report a case of Blymphoproliferative disorder with villous lymphocytes harboring t(8;14)(q24;q32) chromosome translocation that we inclined to SLVL with a prolymphocytic transformation. A 73-year-old female showed marked hepatosplenomegaly and high lymphocytosis (lymphocytes > 200 × 10(9)/L)...
October 27, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/29070144/-evaluation-of-ncd64-and-cd14-index-as-diagnostic-markers-for-hematologic-tumors-combined-with-bacterial-infection
#12
Yuan-Yuan Ma, Qimuge Saiyin
OBJECTIVE: To explore the value of both CD64(nCD64 IND) and CD14 (CD14 IND) Index of peripheral blood neutrophils as the diagnostic markers of hematologic tumors combined with bacterial infection. METHODS: Sixty-four patients with hematological tumors were selected and divided into the group complicated with bacterial infection (infection group, 33 cases) and the non-infection group (31 cases), the flow cytometry was be used to detect the related peripheral blood markers CD64 and CD14, the nCD64 IND and CD14 IND were calculated, and the peripheral blood WBC count, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were determined at the same time...
October 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29064053/correlation-of-circulating-cd64-cd163-monocyte-ratio-and-stroma-peri-tumoral-cd163-monocyte-density-with-human-papillomavirus-infected-cervical-lesion-severity
#13
Piyawut Swangphon, Chamsai Pientong, Nuchsupha Sunthamala, Sureewan Bumrungthai, Miyuki Azuma, Pilaiwan Kleebkaow, Thumwadee Tangsiriwatthana, Ussanee Sangkomkamhang, Bunkerd Kongyingyoes, Tipaya Ekalaksananan
HPV infected cervical cells secrete mediators that are gradually changed and have influence on infiltrating M2 phenotypic monocytes in cervical lesions. However, profiles of circulating immune cells in women with cervical lesions and M2 phenotypic monocyte activity in HPV infected cervical lesions are limited. This study aimed to investigate circulating monocyte populations correlated with M2 phenotype density and its activity in HPV infected cervical lesions. HPV DNA was investigated in cervical tissues using PCR...
October 24, 2017: Cancer Microenvironment: Official Journal of the International Cancer Microenvironment Society
https://www.readbyqxmd.com/read/29051759/ifn%C3%AE-enhances-cd64-potentiated-phagocytosis-of-treponema-pallidum-opsonized-with-human-syphilitic-serum-by-human-macrophages
#14
Kelly L Hawley, Adriana R Cruz, Sarah J Benjamin, Carson J La Vake, Jorge L Cervantes, Morgan LeDoyt, Lady G Ramirez, Daniza Mandich, Mary Fiel-Gan, Melissa J Caimano, Justin D Radolf, Juan C Salazar
Syphilis is a multi-stage, sexually transmitted disease caused by the spirochete Treponema pallidum (Tp). Considered broadly, syphilis can be conceptualized as a dualistic process in which spirochete-driven inflammation, the cause of clinical manifestations, coexists to varying extents with bacterial persistence. Inflammation is elicited in the tissues, along with the persistence of spirochetes to keep driving a robust immune response while evading host defenses; this duality is best exemplified during the florid, disseminated stage called secondary syphilis (SS)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28984423/the-triple-negative-cd34-hla-dr-cd11b-profile-rapidly-and-specifically-identifies-an-acute-promyelocytic-leukemia
#15
K Rahman, R Gupta, M K Singh, M K Sarkar, A Gupta, S Nityanand
INTRODUCTION: The genetic testing to confirm or rule out an acute promyelocytic leukemia (APL) typically takes a minimum of 24-72 hours. Flow cytometric immunophenotyping (FCI) on the other hand provides rapid and objective information to differentiate APL from non-APL. METHODS: FCI features, with single-tube 8-color combination using CD45, CD34, HAL-DR, CD11b, CD13, CD33, and CD117 and CD64, were compared for the 30 consecutive APL and 30 non-APL acute myeloid leukemia (AML) cases which morphologically mimicked an APL...
October 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28972490/phagocytosis-and-expression-of-fcg-receptors-and-cd180-on-monocytes-in-chronic-lymphocytic-leukemia
#16
T Tsertsvadze, N Mitskevich, A Bilanishvili, D Girdaladze, N Porakishvili
Chronic lymphocytic leukaemia (CLL) is a lymphoproliferative disease characterised by accumulation of monoclonal CD19+CD5+CD23+ lymphocytes in the peripheral blood and bone marrow. CLL is the most common type of the adult leukemia in the Western world. The disease is incurable, albeit there are new molecular and immunotherapy methods currently available in conjunction with chemotherapy, leading to the "precision therapy". The majority of immunotherapeutic approaches are based on the ability of therapeutic antibodies (Rituximab, Alemtuzumab) to mobilize anti-tumour potential of the Natural Killer cells and macrophages/monocytes through their Fcg-receptors (FcγR)...
September 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28965741/novel-biomarkers-for-sepsis-a-narrative-review
#17
Frederik Fruergaard Larsen, J Asger Petersen
BACKGROUND: Sepsis is a prevalent condition among hospitalized patients that carries a high risk of morbidity and mortality. Rapid recognition of sepsis as the cause of deterioration is desirable, so effective treatment can be initiated rapidly. Traditionally, diagnosis was based on presence of two or more positive SIRS criteria due to infection. However, recently published sepsis-3 criteria put more emphasis on organ dysfunction caused by infection in the definition of sepsis. Regardless of this, no gold standard for diagnosis exist, and clinicians still rely on a number of traditional and novel biomarkers to discriminate between patients with and without infection, as the cause of deterioration...
September 28, 2017: European Journal of Internal Medicine
https://www.readbyqxmd.com/read/28898742/neutrophil-cd64-c-reactive-protein-and-procalcitonin-in-the-identification-of-sepsis-in-the-icu-post-test-probabilities
#18
Joel Jämsä, Tero Ala-Kokko, Virva Huotari, Pasi Ohtonen, Eeva-Riitta Savolainen, Hannu Syrjälä
PURPOSE: We were interested in whether C-reactive protein (CRP) and procalcitonin (PCT) distinguish sepsis from non-septic controls and whether a combination of CRP, PCT, and neutrophil CD64 improves identification of sepsis in the intensive care unit (ICU). MATERIALS AND METHODS: We analyzed the CRP and PCT concentrations from 27 patients with sepsis and 15 ICU controls. In addition, CD64 on neutrophils was measured using quantitative flow cytometry. We present a multiple marker analysis for sepsis diagnostics combining neutrophil CD64, CRP, and PCT using post-test analysis...
August 31, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28895912/cd64-an-attractive-immunotherapeutic-target-for-m1-type-macrophage-mediated-chronic-inflammatory-diseases
#19
REVIEW
Olusiji A Akinrinmade, Shivan Chetty, Adebukola K Daramola, Mukit-Ul Islam, Theo Thepen, Stefan Barth
To date, no curative therapy is available for the treatment of most chronic inflammatory diseases such as atopic dermatitis, rheumatoid arthritis, or autoimmune disorders. Current treatments require a lifetime supply for patients to alleviate clinical symptoms and are unable to stop the course of disease. In contrast, a new series of immunotherapeutic agents targeting the Fc γ receptor I (CD64) have emerged and demonstrated significant clinical potential to actually resolving chronic inflammation driven by M1-type dysregulated macrophages...
September 12, 2017: Biomedicines
https://www.readbyqxmd.com/read/28791853/neutrophil-cd64-as-a-diagnostic-marker-for-neonatal-sepsis-meta-analysis
#20
Ji Dai, Wenjie Jiang, Zhigang Min, Jian Yang, Yongfei Tan, Tieliang Ma, Zhijun Ge
BACKGROUND: Neutrophil CD64 (nCD64) is a promising marker for diagnosing bacterial infections. Several studies have investigated the performance of nCD64 for diagnosing neonatal sepsis and the results are variable. Interest in nCD64 for detecting serious bacterial infections is increasing rapidly. OBJECTIVES: The aim of the present study was to carry out a meta-analysis to systematically evaluate the diagnostic accuracy of nCD64 in neonatal sepsis. As far as the authors know, no previous studies have undertaken this...
March 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
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