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Homocysteine and gene expression

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https://www.readbyqxmd.com/read/28189056/metabolic-responses-to-intestine-regeneration-in-sea-cucumbers-apostichopus-japonicus
#1
Lina Sun, Jingchun Sun, Qinzeng Xu, Xiaoni Li, Libin Zhang, Hongsheng Yang
Sea cucumbers are excellent models for studying organ regeneration due to their striking capacity to regenerate most of their viscera after evisceration. In this study, we applied NMR-based metabolomics to determine the metabolite changes that occur during the process of intestine regeneration in sea cucumbers. Partial least-squares discriminant analysis showed that there was significant differences in metabolism between regenerative intestines at 3, 7, and 14days post evisceration (dpe) and normal intestines...
February 4, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28179424/altered-methylation-of-specific-dna-loci-in-the-liver-of-bhmt-null-mice-results-in-repression-of-iqgap2-and-f2rl2-and-is-associated-with-development-of-preneoplastic-foci
#2
Daniel S Lupu, Luz D Orozco, Ying Wang, John M Cullen, Matteo Pellegrini, Steven H Zeisel
Folate B12-dependent remethylation of homocysteine is important, but less is understood about the importance of the alternative betaine-dependent methylation pathway-catalyzed by betaine-homocysteine methyltransferase (BHMT)-for establishing and maintaining adequate DNA methylation across the genome. We studied C57Bl/6J Bhmt (betaine-homocysteine methyltransferase)-null mice at age 4, 12, 24, and 52 wk (N = 8) and observed elevation of S-adenosylhomocysteine concentrations and development of preneoplastic foci in the liver (increased placental glutathione S-transferase and cytokeratin 8-18 activity; starting at 12 wk)...
February 8, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28165157/atf6%C3%AE-based-fine-tuning-of-the-unfolded-protein-response-enhances-therapeutic-antibody-productivity-of-chinese-hamster-ovary-cells
#3
Lisa A Pieper, Michaela Strotbek, Till Wenger, Monilola A Olayioye, Angelika Hausser
The dynamics of protein folding and secretion are key issues in improving the productivity and robustness of Chinese Hamster Ovary (CHO) producer cells. High recombinant protein secretion in CHO producer clones triggers the activation of the unfolded protein response (UPR), an intracellular response to the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum (ER). We previously reported that the human microRNA (miRNA) miR-1287 enhances productivity in IgG expressing CHO cells (CHO-IgG)...
February 6, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28161165/supplemental-methionine-choline-or-taurine-alter-in-vitro-gene-network-expression-of-polymorphonuclear-leukocytes-from-neonatal-holstein-calves
#4
M K Abdelmegeid, M Vailati-Riboni, A Alharti, F Batistel, J J Loor
Isolated PMNL from neonatal calves were used to evaluate the effect of Met, choline, and taurine supplementation on mRNA expression of genes related to the Met cycle and innate immunity. Five neonatal Holstein calves (3 wk old) were used for PMNL isolation and in vitro culture. The selected genes were related to the 1-carbon and Met cycles, cell signaling and cytokine mediators, inflammation, antimicrobial and killing mechanism associated genes, immune mediators, adhesion, and pathogen recognition. The results indicated that supplementation of Met, choline, and taurine increased homocysteine synthesis through upregulation of SAHH...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28152052/choline-and-methionine-differentially-alter-methyl-carbon-metabolism-in-bovine-neonatal-hepatocytes
#5
Tawny L Chandler, Heather M White
Intersections in hepatic methyl group metabolism pathways highlights potential competition or compensation of methyl donors. The objective of this experiment was to examine the expression of genes related to methyl group transfer and lipid metabolism in response to increasing concentrations of choline chloride (CC) and DL-methionine (DLM) in primary neonatal hepatocytes that were or were not exposed to fatty acids (FA). Primary hepatocytes isolated from 4 neonatal Holstein calves were maintained as monolayer cultures for 24 h before treatment with CC (61, 128, 2028, and 4528 μmol/L) and DLM (16, 30, 100, 300 μmol/L), with or without a 1 mmol/L FA cocktail in a factorial arrangement...
2017: PloS One
https://www.readbyqxmd.com/read/28126648/maternal-consumption-of-low-isoflavone-soy-protein-isolate-alters-hepatic-gene-expression-and-liver-development-in-rat-offspring
#6
Sae Bom Won, Anna Han, Young Hye Kwon
In utero environment is known to affect fetal development. Especially, the distinct fetal programming of carcinogenesis was reported in offspring exposed to maternal diets containing soy protein isolate (SPI) or genistein. Therefore, we investigated whether maternal consumption of low-isoflavone SPI or genistein alters hepatic gene expression and liver development in rat offspring. Female Sprague-Dawley rats were fed a casein diet, a low-isoflavone SPI diet or a casein diet supplemented with genistein (250 mg/kg diet) for 2 weeks before mating and throughout pregnancy and lactation...
January 12, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28118529/slc25a26-overexpression-impairs-cell-function-via-mtdna-hypermethylation-and-rewiring-of-methyl-metabolism
#7
Alessio Menga, Erika M Palmieri, Antonia Cianciulli, Vittoria Infantino, Massimiliano Mazzone, Antonio Scilimati, Ferdinando Palmieri, Alessandra Castegna, Vito Iacobazzi
Cancer cells down-regulate different genes to take a selective advantage towards invasiveness and/or metastasis formation. The SLC25A26 gene, encoding for the mitochondrial carrier that catalyzes the import of S-adenosylmethionine (SAM) into the mitochondrial matrix required for mitochondrial methylation processes, is down-regulated in cervical cancer cells. In this study we show that SLC25A26 is down-regulated due to gene promoter hypermethylation, as a mechanism to promote cell survival and proliferation...
January 24, 2017: FEBS Journal
https://www.readbyqxmd.com/read/27965408/prenatal-betaine-exposure-modulates-hypothalamic-expression-of-cholesterol-metabolic-genes-in-cockerels-through-modifications-of-dna-methylation
#8
Abdulrahman A Idriss, Yun Hu, Qinwei Sun, Longfei Jia, Yimin Jia, Nagmeldin A Omer, Halima Abobaker, Ruqian Zhao
Cholesterol is essential for neuronal development and brain function. Previously we reported that in ovo administration of betaine modulates hepatic cholesterol metabolism in the chicken, yet it remains unknown whether maternal betaine affects the cholesterol content and the expression of cholesterol metabolic genes in chicken hypothalamus. In this study, eggs were injected with saline or betaine at 2.5 mg/egg, and the hatchlings were raised under the same condition until 64 d of age. Maternal betaine significantly (P = 0...
December 13, 2016: Poultry Science
https://www.readbyqxmd.com/read/27890035/epigenetics-in-type-1-diabetes-tnfa-gene-promoter-methylation-status-in-chilean-patients-with-type-1-diabetes-mellitus
#9
Viviana Arroyo-Jousse, Diego F Garcia-Diaz, Ethel Codner, Francisco Pérez-Bravo
TNF-α is a pro-inflammatory cytokine that is involved in type 1 diabetes (T1D) pathogenesis. The TNFa gene is subject of epigenetic regulation in which folate and homocysteine are important molecules because they participate in the methionine cycle where the most important methyl group donor (S-adenosylmethionine) is formed. We investigated whether TNFa gene promoter methylation status in T1D patients was related to blood folate, homocysteine and TNF-α in a transversal case-control study. We studied T1D patients (n 25, mean=13·7 years) and healthy control subjects (n 25, mean=31·1 years), without T1D and/or other autoimmune diseases or direct family history of these diseases...
December 2016: British Journal of Nutrition
https://www.readbyqxmd.com/read/27848249/cystathionine-%C3%AE-lyase-deficiency-exacerbates-ccl4-induced-acute-hepatitis-and-fibrosis-in-the-mouse-liver
#10
Lei Ci, Xing Yu Yang, Xiao Wen Gu, Qing Li, Yang Guo, Zi Ping Zhou, Meng Jie Zhang, Jia Hao Shi, Hua Yang, Zhu Gang Wang, Jian Fei
AIMS: The present study examined the role of cystathionine γ-lyase (CSE) in CCl4- induced liver damage. RESULTS: A CSE gene knock-out and luciferase gene knock-in mouse model was constructed to study the function of CSE and trace its expression in living status. CCl4 or LPS markedly downregulated CSE expression in the liver of mice. CSE-deficient mice showed increased serum alanine aminotransferase and aspartate aminotransferase levels, and liver damage after CCl4 challenge, whereas albumin and endogenous H2S levels decreased significantly...
November 16, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/27808252/functional-variants-of-the-5-methyltetrahydrofolate-homocysteine-methyltransferase-gene-significantly-increase-susceptibility-to-prostate-cancer-results-from-an-ethnic-han-chinese-population
#11
Yuan-Yuan Qu, Shu-Xian Zhou, Xuan Zhang, Rui Zhao, Cheng-Yuan Gu, Kun Chang, Xiao-Qun Yang, Hua-Lei Gan, Bo Dai, Hai-Liang Zhang, Guo-Hai Shi, Yao Zhu, Ding-Wei Ye, Jian-Yuan Zhao
Aberrant DNA methylation has been implicated in prostate carcinogenesis. The one-carbon metabolism pathway and related metabolites determine cellular DNA methylation and thus is thought to play a pivotal role in PCa occurrence. This study aimed to investigate the contribution of genetic variants in one-carbon metabolism genes to prostate cancer (PCa) risk and the underlying biological mechanisms. In this hospital-based case-control study of 1817 PCa cases and 2026 cancer-free controls, we genotyped six polymorphisms in three one-carbon metabolism genes and assessed their association with the risk of PCa...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27771368/paraoxonase-and-atherosclerosis-related-cardiovascular-diseases
#12
REVIEW
Dimitry A Chistiakov, Alexandra A Melnichenko, Alexander N Orekhov, Yuri V Bobryshev
In humans, three paraoxonase (PON1, PON2, and PON3) genes are clustered on chromosome 7 at a locus that spans a distance around 170 kb. These genes are highly homologous to each other and have a similar protein structural organization. PON2 is the intracellular enzyme, which is expressed in many tissues and organs, while two other members of PON gene family are produced by liver and associate with high density lipoprotein (HDL). The lactonase activity is the ancestral. Besides lactones and organic phosphates, PONs can hydrolyze and therefore detoxify oxidized low density lipoprotein and homocysteine thiolactone, i...
January 2017: Biochimie
https://www.readbyqxmd.com/read/27766206/transcript-profiling-in-the-liver-of-early-lactating-dairy-cows-fed-conjugated-linoleic-acid
#13
Robert Ringseis, Wilhelm Windisch, Klaus Eder
In the present study, transcript profiling was carried out in liver biopsies from high-yielding dairy cows at week 5 of lactation in order to identify genes and pathways regulated by feeding rumen-protected conjugated linoleic acid (CLA) during the transition period. Analysis of a bovine whole genome microarray revealed a total number of 130 annotated differentially expressed genes (DEGs) in the liver between cows of the CLA group and the control group (filter: P < 0.05 and fold change (FC) ≥ 1.3 or ≤- 1...
December 2016: Genomics Data
https://www.readbyqxmd.com/read/27713121/microrna-130a-alleviates-human-coronary-artery-endothelial-cell-injury-and-inflammatory-responses-by-targeting-pten-via-activating-pi3k-akt-enos-signaling-pathway
#14
Chun-Li Song, Bin Liu, Yong-Feng Shi, Ning Liu, You-You Yan, Ji-Chang Zhang, Xin Xue, Jin-Peng Wang, Zhuo Zhao, Jian-Gen Liu, Yang-Xue Li, Xiao-Hao Zhang, Jun-Duo Wu
Our study aims to investigate the roles of microRNA-130a (miR-130a) in human coronary artery endothelial cells (HCAECs) injury and inflammatory responses by targeting PTEN through the PI3K/Akt/eNOS signaling pathway. HCAECs were treated with 1.0 mmol/L homocysteine (HCY) and assigned into eight groups: the blank group, the negative control (NC) group, the miR-130a mimics group, the miR-130a inhibitors group, the si-PTEN group, the Wortmannin group, the miR-130a inhibitors + si-PTEN group and the miR-130a mimics + Wortmannin group...
October 4, 2016: Oncotarget
https://www.readbyqxmd.com/read/27693409/serum-paraoxonase-activity-in-relation-to-lipid-profile-in-age-related-macular-degeneration-patients
#15
Kannadasan AnandBabu, S R Bharathidevi, Sarangapani Sripriya, Parveen Sen, Vadivelu Jaya Prakash, Appukuttan Bindu, Natarajan Viswanathan, Narayanasamy Angayarkanni
Age-related Macular Degeneration (AMD) is a multifactorial disease causing visual impairment in old age. Oxidative stress is one of the main contributors for the disease progression. Paraoxonase (PON), a HDL-resident antioxidant enzyme which removes oxidized low density lipoprotein (oxLDL), which is not studied much in AMD. This study assesses the PON activities in relation to the lipid status and genetic variants in AMD patients. In this prospective case-control study, a total of 48 AMD patients and 30 unrelated healthy controls were recruited...
September 28, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27686255/altered-regulation-of-the-spry2-dyrk1a-pp2a-triad-by-homocysteine-impairs-neural-progenitor-cell-proliferation
#16
Luis G Rabaneda, Noelia Geribaldi-Doldán, Maribel Murillo-Carretero, Manuel Carrasco, José M Martínez-Salas, Cristina Verástegui, Carmen Castro
Hyperhomocysteinemia reduces neurogenesis in the adult mouse brain. Homocysteine (Hcy) inhibits postnatal neural progenitor cell (NPC) proliferation by specifically impairing the fibroblast growth factor receptor (FGFR)-Erk1/2-cyclin E signaling pathway. We demonstrate herein that the inhibition of FGFR-dependent NPC proliferation induced by Hcy is mediated by its capacity to alter the cellular methylation potential. Our results show that this alteration modified the expression pattern and activity of Sprouty2 (Spry2), a negative regulator of the above mentioned pathway...
December 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27677203/antioxidant-mechanism-of-betaine-without-free-radical-scavenging-ability
#17
Mengmeng Zhang, Hong Zhang, Huixian Li, Furao Lai, Xiaofeng Li, Yuqian Tang, Tian Min, Hui Wu
Betaine (BET) is a native compound widely studied as an antioxidant in agriculture and human health. However, the antioxidant mechanism of BET remains unclear. In this research, radical scavenging assays showed that BET had little free radical scavenging activity. However, the antioxidant activity of BET was confirmed by cellular antioxidant activity (CAA) and erythrocyte hemolysis assays. The results of quantitative PCR (qPCR) and enzyme activity determination kits showed that the antioxidant activity of BET was not due to the gene expression and activity of antioxidases...
October 12, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27587023/activation-of-the-eif2%C3%AE-atf4-and-atf6-pathways-in-du-145-cells-by-boric-acid-at-the-concentration-reported-in-men-at-the-us-mean-boron-intake
#18
Sarah E Kobylewski, Kimberly A Henderson, Kristin E Yamada, Curtis D Eckhert
Fruits, nuts, legumes, and vegetables are rich sources of boron (B), an essential plant nutrient with chemopreventive properties. Blood boric acid (BA) levels reflect recent B intake, and men at the US mean intake have a reported non-fasting level of 10 μM. Treatment of DU-145 prostate cancer cells with physiological concentrations of BA inhibits cell proliferation without causing apoptosis and activates eukaryotic initiation factor 2 (eIF2α). EIF2α induces cell differentiation and protects cells by redirecting gene expression to manage endoplasmic reticulum stress...
September 1, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27509954/methyl-donor-status-influences-dnmt-expression-and-global-dna-methylation-in-cervical-cancer-cells
#19
Natwadee Poomipark, Janet E Flatley, Marilyn H Hill, Barbara Mangnall, Elnaz Azar, Peter Grabowski, Hilary J Powers
BACKGROUND: Methyl donor status influences DNA stability and DNA methylation although little is known about effects on DNA methyltransferases. The aim of this study was to determine whether methyldonor status influences DNA methyltransferase (Dnmt) gene expression in cervical cancer cells, and if so, whether there are associated effects on global DNA methylation. MATERIALS AND METHODS: The human cervical cancer cell line, C4 II, was grown in complete medium and medium depleted of folate (FM+) and folate and methionine (FM)...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27474977/the-effect-of-increasing-concentrations-of-dl-methionine-and-2-hydroxy-4-methylthio-butanoic-acid-on-hepatic-genes-controlling-methionine-regeneration-and-gluconeogenesis
#20
Qian Zhang, Sandra J Bertics, N Daniel Luchini, Heather M White
Metabolizable methionine (Met) concentrations can be increased by feeding rumen-protected dl-Met or the isopropyl ester of 2-hydroxy-4-(methylthio) butanoic acid (HMBi). Hepatic responses to increasing concentrations of metabolizable Met as a result of supplementation of different Met sources have not been comparatively examined. The objective of this experiment was to examine the regulation of key genes for Met metabolism, gluconeogenesis, and fatty acid oxidation in response to increasing concentrations of dl-Met or 2-hydroxy-4-(methylthio) butanoic acid (HMB) in bovine primary hepatocytes...
October 2016: Journal of Dairy Science
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