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Adel M A Assiri, Hala F M Kamel, Abeer A ALrefai
The interaction of advanced glycation end products (AGE) and their receptors promote vascular complications of diabetes in hemodialysis (HD) patients. The soluble form of the receptor for the advanced glycation end-products (sRAGE) has been studied as a vascular biomarker in various diseases with controversial results. Our aim was to evaluate the association of the serum levels of the AGEs and their receptor sRAGE with cardiovascular disease (CVD) and the cardiovascular risk factors among HD patients. There were 130 HD patients and 80 age and gender matched control subjects were involved; 31...
May 22, 2018: Medical Sciences: Open Access Journal
Julia Magdalena Kubiak, Per Medbøe Thorsby, Elena Kamycheva, Rolf Jorde
OBJECTIVE: Low serum 25(OH)D levels are associated with cardiovascular disease (CVD) and some of its risk factors. However, in interventional studies the effects of vitamin D supplementation have been uncertain, possibly due to inclusion of vitamin D sufficient subjects. Our aim was therefore to examine effects of vitamin D supplementation on CVD risk factors in vitamin D insufficient subjects. DESIGN: Double-blinded randomized controlled trial. METHODS: A four months interventional study with high dose vitamin D (100 000 IU loading dose, followed by 20 000 IU/week) or placebo with measurements of blood pressure, lipids (total-, LDL- and HDL-cholesterol, triglycerides, apolipoproteins A1 and B), and glucose metabolism parameters (blood glucose, HbA1c, sRAGE, insulin, C-peptide, and HOMA-IR)...
May 15, 2018: Endocrine Connections
Bashar S Staitieh, Eduardo E Egea, Xian Fan, Adaugo Amah, David M Guidot
BACKGROUND: Alcohol significantly impairs antioxidant defenses and innate immune function in the lung and increases matrix metalloproteinase 9 (MMP-9) activity. The receptor for advanced glycation end products (RAGE) is a well-characterized marker of lung injury that is cleaved by MMP-9 into soluble RAGE and has not yet been examined in the alcoholic lung. We hypothesized that chronic alcohol ingestion would impair RAGE signaling via MMP-9 in the alveolar macrophage and thereby impair innate immune function...
May 2018: American Journal of the Medical Sciences
C J Boos, C M Lamb, M Midwinter, A Mellor, D R Woods, M Howley, T Stansfield, M Foster, J P O'hara
The binding of high-mobility group box-1 (HMGB-1) to the membrane receptor for advanced glycation end-products (mRAGE) is a key early mediator of non-infectious inflammation and its triggers include ischaemia/hypoxia. The effects of acute hypoxia on soluble RAGE (sRAGE) are unknown. Fourteen healthy adults (50% women; 26.6+/-3.8 years) were assessed at baseline normoxia (T0), followed by four time-points (T90, 95, 100 and 180 minutes) over three hours of continuous normobaric hypoxia (NH, 4450m equivalent) and again 60 minutes after return to normoxia (T240)...
May 10, 2018: Physiological Research
Alberto García-Salido, Gustavo Melen, Vanesa Gómez-Piña, Gonzalo Oñoro-Otero, Ana Serrano-González, Juan Casado-Flores, Manuel Ramírez
BACKGROUND: The receptor for advanced glycation end products (RAGE) has a critical role in the pathogenesis of inflammation. In healthy children, its basal expression on the peripheral blood mononuclear cell (PBMC) and the basal circulating soluble RAGE (sRAGE) levels are unknown. The aim of this study was to describe both. METHODS: This is a monocentric, observational and descriptive study of samples obtained from healthy children. The RAGE expression on PBMC was analyzed using flow cytometry...
May 3, 2018: Journal of Pediatric Endocrinology & Metabolism: JPEM
Sierra A Patterson, Gagan Deep, Tina E Brinkley
Exosomes are nanovesicles that participate in cell-to-cell communication and are secreted by a variety of cells including neurons. Recent studies suggest that neuronally-derived exosomes are detectable in plasma and that their contents likely reflect expression of various biomarkers in brain tissues. The receptor for advanced glycation endproducts (RAGE) has been implicated in the pathophysiology of Alzheimer's disease (AD) and is increased in brain regions affected by AD. The goal of our project was to determine whether RAGE is present in plasma exosomes, and specifically exosomes derived from neurons...
April 24, 2018: Biochemical and Biophysical Research Communications
Sook Young Kim, Myoungsun Son, Sang Eun Lee, In Ho Park, Man Sup Kwak, Myeonggil Han, Hyun Sook Lee, Eun Sook Kim, Jae-Young Kim, Jong Eun Lee, Ji Eun Choi, Betty Diamond, Jeon-Soo Shin
High-mobility group box 1 (HMGB1), a well-known danger-associated molecular pattern molecule, acts as a pro-inflammatory molecule when secreted by activated immune cells or released after necrotic cell damage. HMGB1 binds to immunogenic bacterial components and augments septic inflammation. In this study, we show how HMGB1 mediates complement activation, promoting sterile inflammation. We show that HMGB1 activates the classical pathway of complement system in an antibody-independent manner after binding to C1q...
2018: Frontiers in Immunology
Kelly N Z Fuller, Edwin R Miranda, John P Thyfault, Jill K Morris, Jacob M Haus
Although there is evidence for metabolic dysfunction and chronic inflammation in Alzheimer's disease (AD), circulating levels of soluble receptor for advanced glycation end products (sRAGE) and the receptor for advanced glycation end products (RAGE) ligand S100B have not been characterized. sRAGE is an important mediator in disease as it can act as a ligand decoy for RAGE and attenuate downstream inflammatory signaling. Cognitively healthy elderly and AD participants with and without type 2 diabetes ( n = 135) were stratified according to the clinical dementia rating (CDR; 0 = normal cognition (NC); ≥0...
2018: Mediators of Inflammation
Mahnoosh Rahimi, Sarah Saadat Aghabozorg Afjeh, Mir Davood Omrani, Shahram Arsang-Jang, Maziar Ganji, Rezvan Noroozi, Mohammad Taheri, Soudeh Ghafouri-Fard
BACKGROUND/AIMS: Multiple sclerosis (MS) is a chronic inflammatory disorder of the central nervous system. Considering the role of immune system in its pathogenesis, researchers have focused on evaluation of the expression of immune-related genes or proteins in MS patients. Among proteins whose participation in inflammatory process has been documented is the receptor for advanced glycation end products (RAGE). METHODS: In the present study, we compared RAGE transcript levels by means of quantitative real-time PCR as well as the serum level of soluble RAGE (sRAGE) by means of enzyme- linked immunosorbent assay (ELISA) in 50 IFNβ-1a responsive relapsing-remitting MS patients when compared with age and sex-matched healthy subjects...
March 28, 2018: Cellular Physiology and Biochemistry
Foo Nian Wong, Kek Heng Chua, Jin Ai Mary Anne Tan, Chew Ming Wong, Umah Rani Kuppusamy
Background: Chronic kidney disease (CKD) is characterised by long-term kidney damage and renal function decline. Diabetic CKD is the principal subtype of kidney disease in Malaysia and is associated with oxidative stress which plays an important role in development and progression of the disease. Glycaemic control slows down the progression of diabetic complications, including diabetic CKD. However, the implication of glycaemic control on enzymatic antioxidants and soluble RAGE (sRAGE) in CKD patients remains elusive...
2018: PeerJ
Ines Knani, Hassan Bouzidi, Saoussen Zrour, Naceur Bergaoui, Mohamed Hammami, Mohsen Kerkeni
Background: The contribution of methylglyoxal (MGO) and soluble receptor for advanced glycation end products (sRAGE) in the presence of rheumatoid arthritis (RA) is still unknown. We investigated whether serum MGO and sRAGE were related to the presence of disease activity in RA. Methods: 80 patients with RA and 30 control subjects were included in a cross-sectional study. The severity of RA was assessed using the disease activity score for 28 joints (DAS28). Serum MGO and sRAGE were measured by ELISA...
2018: Disease Markers
Donna L White, Ron C Hoogeveen, Liang Chen, Peter Richardson, Milan Ravishankar, Preksha Shah, Lesley Tinker, Thomas Rohan, Eric A Whitsel, Hashem B El-Serag, Li Jiao
Advanced glycation end products (AGEs) dysregulate adipokines and induce inflammation by binding to their adipocyte receptor (RAGE). Soluble RAGE (sRAGE) prevents AGEs/RAGE signaling. We performed a nested case-control study of the association between sRAGE, adipokines, and incident pancreatic cancer risk in the prospective Women's Health Initiative Study. We individually matched controls (n = 802) to cases (n = 472) on age, race, and blood draw date. We evaluated serum concentrations of sRAGE, adiponectin, leptin, monocyte chemotactic protein 1 (MCP1), and plasminogen activator inhibitor-1 (PAI1) using immunoassay...
March 23, 2018: Cancer Medicine
Z Merhi, E Buyuk, M J Cipolla
STUDY QUESTION: Does vitamin D attenuate the adverse effects of advanced glycation end products (AGEs) on steroidogenesis by human granulosa cells (GCs)? SUMMARY ANSWER: AGEs alter the expression of genes important in steroidogenesis while 1,25-dihydroxyvitamin D3 (vit D3) in vitro attenuates some of the actions of AGEs on steroidogenic gene expression, possibly by downregulating the expression of the pro-inflammatory cell membrane receptor for AGEs (RAGE). WHAT IS KNOWN ALREADY: Vitamin D attenuates the pro-inflammatory effects of AGEs in non-ovarian tissues...
June 1, 2018: Molecular Human Reproduction
John Rene Labib, Sally Kamal Ibrahem, Hala Mohamed Sleem, Mohamed M Ismail, Shaimaa A M Abd El Fatah, Marwa Rashad Salem, Amaal A Abdelaal, Hadeel Al-Hanafi
Early identification of acute lung injury (ALI) in pediatric patients at risk of mortality is important for improving outcome.Assessment of soluble form of receptor for advanced glycation end products (sRAGE) as a valid biomarker for diagnosis of ALI among critically ill, pediatric patients in addition to correlating levels of sRAGE and different outcomes of those patients.A Hospital-based case-control study was conducted in pediatric intensive care units (PICUs) at Cairo University Hospital, along a period of 6 months...
March 2018: Medicine (Baltimore)
F Zhou, Y Tan, X-H Chen, F-L Wu, D-J Yang, X-W Zhang, X-M Wu, Y-Q Deng
OBJECTIVE: To study the improving effect of atorvastatin on plaque stability in diabetes mellitus (DM) mice complicated with atherosclerosis. MATERIALS AND METHODS: Apolipoprotein E (ApoE)-/- mice were used to establish the DM mouse model. Half of the mice received atorvastatin after successful modeling. ApoE-/- and C57BL/6J mice were used as controls. Oil red O staining and Masson staining were performed to detect the lipid and collagen components in mice. Immunohistochemical assay was used to observe the expressions of smooth muscle cell (SMC) and Ly-6c...
February 2018: European Review for Medical and Pharmacological Sciences
Felix C F Schmitt, Eduardo Salgado, Janina Friebe, Thomas Schmoch, Florian Uhle, Thomas Fleming, Johanna Zemva, Lars Kihm, Christian Nusshag, Christian Morath, Martin Zeier, Thomas Bruckner, Arianeb Mehrabi, Peter P Nawroth, Markus A Weigand, Stefan Hofer, Thorsten Brenner
A prolonged cold ischaemia time (CIT) is suspected to be associated with an increased ischaemia and reperfusion injury (IRI) resulting in an increased damage to the graft. In total, 91 patients were evaluated for a delayed graft function within 7 days after kidney transplantation (48 deceased, 43 living donors). Blood and urine samples were collected before, immediately after the operation, and 1, 3, 5, 7 and 10 days later. Plasma and/or urine levels of total keratin 18 (total K18), caspase-cleaved keratin 18 (cc K18), the soluble receptor for advanced glycation end products (sRAGE), tissue inhibitor of metalloproteinase-2 (TIMP-2) and insulin-like growth factor-binding protein-7 (IGFBP7) were measured...
March 5, 2018: Transplant International: Official Journal of the European Society for Organ Transplantation
Frank Klont, Simon D Pouwels, Jos Hermans, Nico C van de Merbel, Péter Horvatovich, Nick H T Ten Hacken, Rainer Bischoff
The study of proteins is central to unraveling (patho)physiological processes and has contributed greatly to our understanding of biological systems. Corresponding studies often employ procedures to enrich proteins from their biological matrix using antibodies or other affinity binders coupled to beads with a large surface area and a correspondingly high binding capacity. Striving for maximal binding capacity may, however, not always be required or desirable, for example for proteins of low abundance. Here we describe a simplified immunoprecipitation in 96-well ELISA format (IPE) approach for fast and easy enrichment of proteins...
May 15, 2018: Talanta
Kailash Prasad, Manish Mishra
Adverse effects of advanced glycation end-products (AGEs) on the tissues are through nonreceptor- and receptor-mediated mechanisms. In the receptor-mediated mechanism, interaction of AGEs with its cell-bound receptor of AGE (RAGE) increases generation of oxygen radicals, activates nuclear factor-kappa B, and increases expression and release of pro-inflammatory cytokines resulting in the cellular damage. The deleterious effects of AGE and AGE-RAGE interaction are coined as "AGE-RAGE stress." The body is equipped with defense mechanisms to counteract the adverse effects of AGE and RAGE through endogenous enzymatic (glyoxalase 1, glyoxalase 2) and AGE receptor-mediated (AGER1, AGER2) degradation of AGE, and through elevation of soluble receptor of AGE (sRAGE)...
March 2018: International Journal of Angiology: Official Publication of the International College of Angiology, Inc
Matthieu Jabaudon, Pauline Berthelin, Thibaut Pranal, Laurence Roszyk, Thomas Godet, Jean-Sébastien Faure, Russell Chabanne, Nathanael Eisenmann, Alexandre Lautrette, Corinne Belville, Raiko Blondonnet, Sophie Cayot, Thierry Gillart, Julien Pascal, Yvan Skrzypczak, Bertrand Souweine, Loic Blanchon, Vincent Sapin, Bruno Pereira, Jean-Michel Constantin
Acute respiratory distress syndrome (ARDS) prediction remains challenging despite available clinical scores. To assess soluble receptor for advanced glycation end-products (sRAGE), a marker of lung epithelial injury, as a predictor of ARDS in a high-risk population, adult patients with at least one ARDS risk factor upon admission to participating intensive care units (ICUs) were enrolled in a multicentre, prospective study between June 2014 and January 2015. Plasma sRAGE and endogenous secretory RAGE (esRAGE) were measured at baseline (ICU admission) and 24 hours later (day one)...
February 8, 2018: Scientific Reports
Shiva Houjeghani, Sorayya Kheirouri, Esmaeil Faraji, Mohammad Asghari Jafarabadi
Considering the pathologic importance of metabolic disturbances, advanced glycation end products (AGEs), and chronic inflammation in diabetes mellitus and ameliorating potentials of l-carnosine in hampering these detritions and because these effects have not been investigated in patients with type 2 diabetes (T2D) so far, we conducted the current study. We hypothesized that l-carnosine would improve glycemic control, lipid profile, AGE, soluble receptor of AGEs (sRAGE), and inflammatory markers. In a randomized, double-blind, placebo-controlled clinical trial, 54 patients with T2D were recruited and assigned into either intervention group (n=27, receiving 2 capsules of l-carnosine 500 mg each) or control group (n=27)...
January 2018: Nutrition Research
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