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Keywords Homocysteine and gene hypometh...

Homocysteine and gene hypomethylation

https://read.qxmd.com/read/32238192/vitamin-b-12-is-neuroprotective-in-experimental-pneumococcal-meningitis-through-modulation-of-hippocampal-dna-methylation
#21
JOURNAL ARTICLE
Karina Barbosa de Queiroz, Vanessa Cavalcante-Silva, Flávia Lombardi Lopes, Gifone Aguiar Rocha, Vânia D'Almeida, Roney Santos Coimbra
BACKGROUND: Bacterial meningitis (BM) causes apoptotic damage to the hippocampus and homocysteine (Hcy) accumulation to neurotoxic levels in the cerebrospinal fluid of children. The Hcy pathway controls bioavailability of methyl, and its homeostasis can be modulated by vitamin B12 , a cofactor of the methionine synthase enzyme. Herein, the neuroprotective potential and the underlying mode of action of vitamin B12 adjuvant therapy were assessed in an infant rat model of BM. METHODS: Eleven-day old rats were intracysternally infected with Streptococcus pneumoniae serotype 3, or saline, treated with B12 or placebo, and, 24 h after infection, their hippocampi were analyzed for apoptosis in the dentate gyrus, sulfur amino acids content, global DNA methylation, transcription, and proximal promoter methylation of candidate genes...
April 1, 2020: Journal of Neuroinflammation
https://read.qxmd.com/read/32193457/induced-dna-hypomethylation-by-folic-acid-deprivation-in-bovine-fibroblast-donor-cells-improves-reprogramming-of-somatic-cell-nuclear-transfer-embryos
#22
JOURNAL ARTICLE
Mina Jozi, Farnoosh Jafarpour, Reza Moradi, Faezeh Ghazvini Zadegan, Khadijeh Karbalaie, Mohammad Hossein Nasr-Esfahani
Aberrant patterns of DNA methylation are consistent events in SCNT derived embryos and mechanistically are believed to be related to abnormal development. While some epigenetic drugs have been used in attempts to improve SCNT efficiency but some concerns remained toward the safety of these drugs on the health of future offspring. Folate is an essential cofactor in one-carbon cycle for conversion of homocysteine to methionine, thereby ensuring supply of SAM, the universal methyl donor for many biological methylation reactions including DNA methylation...
March 19, 2020: Scientific Reports
https://read.qxmd.com/read/32022472/transgenerational-inheritance-of-betaine-induced-epigenetic-alterations-in-estrogen-responsive-igf-2-igfbp2-genes-in-rat-hippocampus
#23
JOURNAL ARTICLE
Yang Yang, Wenduo Jiang, Shu Yang, Fulei Qi, Ruqian Zhao
SCOPE: Betaine serves as a methyl donor for DNA methylation. Here, we sought to investigate the effects of betaine on hippocampal expression of neurogenesis genes and their DNA methylation status across three generations. METHODS AND RESULTS: Pregnant rats (F0) were fed control and betaine-supplemented diets throughout gestation and lactation. Female F1 and F2 offspring at weaning, together with the F0 dams, were used in the study. Hippocampal expression of aromatase, estrogen receptor α and estrogen related receptor β was down-regulated in F1, together with the estrogen-responsive IGF-2/IGFBP2 genes...
February 5, 2020: Molecular Nutrition & Food Research
https://read.qxmd.com/read/31950130/aberrant-dna-methylation-and-mirnas-in-coronary-artery-diseases-and-stroke-a-systematic-review
#24
JOURNAL ARTICLE
Anu Radha Sharma, Umakanth Shashikiran, Abdul Razak Uk, Ranjan Shetty, Kapaettu Satyamoorthy, Padmalatha S Rai
Coronary artery disease (CAD) and ischemic stroke are the two most predominant forms of cardiovascular diseases (CVDs) caused by genetic, epigenetic and environmental risk factors. Although studies on the impact of 'epigenetics' in CVDs is not new, its effects are increasingly being realized as a key regulatory determinant that may drive predisposition, pathophysiology and therapeutic outcome. The most widely studied epigenetic risk factors are regulated by DNA methylation and miRNA expression. To keep pace with growing developments and discoveries, a comprehensive review was performed using Pubmed, Science Direct and Scopus databases to highlight the role of DNA methylation and miRNAs in CAD and stroke subjects...
January 17, 2020: Briefings in Functional Genomics
https://read.qxmd.com/read/31605963/homocysteine-methionine-cycle-is-a-metabolic-sensor-system-controlling-methylation-regulated-pathological-signaling
#25
JOURNAL ARTICLE
Wen Shen, Chao Gao, Ramon Cueto, Lu Liu, Hangfei Fu, Ying Shao, William Y Yang, Pu Fang, Eric T Choi, Qinghua Wu, Xiaofeng Yang, Hong Wang
Homocysteine-Methionine (HM) cycle produces universal methyl group donor S-adenosylmethione (SAM), methyltransferase inhibitor S-adenosylhomocysteine (SAH) and homocysteine (Hcy). Hyperhomocysteinemia (HHcy) is established as an independent risk factor for cardiovascular disease (CVD) and other degenerative disease. We selected 115 genes in the extended HM cycle (31 metabolic enzymes and 84 methyltransferases), examined their protein subcellular location/partner protein, investigated their mRNA levels and mapped their corresponding histone methylation status in 35 disease conditions via mining a set of public databases and intensive literature research...
September 12, 2019: Redox Biology
https://read.qxmd.com/read/30814086/significant-association-of-methylenetetrahydrofolate-dehydrogenase-1-promoter-hypomethylation-with-stroke-in-a-chinese-population-with-primary-hypertension
#26
JOURNAL ARTICLE
Changyi Wang, Guodong Xu, Qi Wen, Xiaolin Peng, Hongen Chen, Jingwen Zhang, Shan Xu, Chunhui Zhang, Min Zhang, Jianping Ma, Zhaohui Hui, Jianhang Du, Guifu Wu, Min Ma
OBJECTIVE: Hypertension and hyperhomocysteinemia are both stroke risk factors. Methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) plays a vital role in one-carbon metabolism via encoding a protein with three distinct enzymatic activities (methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase, and formate-tetrahydrofolate ligase). We aimed to elucidate the association between MTHFD1 promoter methylation and stroke in a Chinese population with primary hypertension...
January 2019: Annals of Clinical and Laboratory Science
https://read.qxmd.com/read/30773021/inhibition-of-s-adenosylhomocysteine-hydrolase-induces-endothelial-dysfunction-via-epigenetic-regulation-of-p66shc-mediated-oxidative-stress-pathway
#27
JOURNAL ARTICLE
Yunjun Xiao, Junjie Xia, Jinquan Cheng, Haiyan Huang, Yani Zhou, Xifei Yang, Xuefen Su, Yuebin Ke, Wenhua Ling
BACKGROUND: Elevated levels of S-adenosylhomocysteine (SAH), the precursor of homocysteine, are positively associated with the risk of cardiovascular disease and with the development and progression of atherosclerosis. However, the role of SAH in endothelial dysfunction is unclear. METHODS: Apolipoprotein E-deficient ( apoE-/ - ) mice received dietary supplementation with the SAH hydrolase (SAHH) inhibitor adenosine dialdehyde or were intravenously injected with a retrovirus expressing SAHH shRNA...
May 7, 2019: Circulation
https://read.qxmd.com/read/30178251/alcohol-induced-epigenetic-changes-in-cancer
#28
JOURNAL ARTICLE
Ramona G Dumitrescu
Chronic, heavy alcohol consumption is associated with serious negative health effects, including the development of several cancer types. One of the pathways affected by alcohol toxicity is the one-carbon metabolism. The alcohol-induced impairment of this metabolic pathway results in epigenetic changes associated with cancer development. These epigenetic changes are induced by folate deficiency and by products of the ethanol metabolism. The changes induced by long-term heavy ethanol consumption result in elevations of homocysteine and S-adenosyl-homocysteine (SAH) and reductions in S-adenosylmethionine (SAM) and antioxidant glutathione (GSH) levels, leading to abnormal promoter gene hypermethylation, global hypomethylation, and metabolic insufficiency of antioxidant defense mechanisms...
2018: Methods in Molecular Biology
https://read.qxmd.com/read/29758262/s-adenosylhomocysteine-hydrolase-participates-in-dna-methylation-inheritance
#29
JOURNAL ARTICLE
V K Chaithanya Ponnaluri, Pierre-Olivier Estève, Cristian I Ruse, Sriharsa Pradhan
DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for mammalian development and maintenance of DNA methylation following DNA replication in cells. The DNA methylation process generates S-adenosyl-l-homocysteine, a strong inhibitor of DNMT1. Here we report that S-adenosylhomocysteine hydrolase (SAHH/AHCY), the only mammalian enzyme capable of hydrolyzing S-adenosyl-l-homocysteine binds to DNMT1 during DNA replication. SAHH enhances DNMT1 activity in vitro, and its overexpression in mammalian cells led to hypermethylation of the genome, whereas its inhibition by adenosine periodate or siRNA-mediated knockdown resulted in hypomethylation of the genome...
July 6, 2018: Journal of Molecular Biology
https://read.qxmd.com/read/28980068/a-unique-methylation-pattern-co-segregates-with-neural-tube-defect-statuses-in-han-chinese-pedigrees
#30
JOURNAL ARTICLE
Ruiping Zhang, Lirong Cao, Yizheng Wang, Yulian Fang, Linsheng Zhao, Weidong Li, Ou-Yan Shi, Chun-Quan Cai
Neural tube defects (NTDs) are a complex trait associated with gene-environment interactions. Folic acid deficiency and planar cell polarity gene mutations account for some NTD cases; however, the etiology of NTDs is still little understood. In this study, in three Han Chinese NTD pedigrees (two with multiple affected children), with no information on folic acid deficiency or supplement, we examined genome-wide methylation profiles of each individual in these families. We further compared methylation status among cases and normal individuals within the pedigrees...
December 2017: Neurological Sciences
https://read.qxmd.com/read/28944836/aberrant-promoter-methylation-of-multiple-genes-in-vsmc-proliferation-induced-by-hcy
#31
JOURNAL ARTICLE
Sheng-Chao Ma, Jian-Cheng Cao, Hui-Ping Zhang, Yun Jiao, Hui Zhang, Yang-Yang He, Yan-Hua Wang, Xiao-Ling Yang, An-Ning Yang, Jue Tian, Ming-Hao Zhang, Xiao-Ming Yang, Guan-Jun Lu, Shao-Ju Jin, Yue-Xia Jia, Yi-Deng Jiang
Vascular smooth muscle cell (VSMC) proliferation is a primary pathological event in atherosclerosis (AS), and homocysteine (Hcy) is an independent risk factor for AS. However, the underlying mechanisms are still lagging. Studies have used the combination of methylation of promoters of multiple genes to diagnose tumors, thus the aim of the current study was to investigate the role of methylation status of several genes in VSMCs treated with Hcy. CpG islands were identified in the promoters of platelet‑derived growth factor (PDGF), p53, phosphatase and tensin homologue on chromosome 10 (PTEN) and mitofusin 2 (MFN2)...
November 2017: Molecular Medicine Reports
https://read.qxmd.com/read/28397458/mapping-of-dna-hypermethylation-and-hypomethylation-induced-by-folate-deficiency-in-sporadic-colorectal-cancer-and-clinical-implication-analysis-of-hypermethylation-pattern-in-cbs-promoter
#32
JOURNAL ARTICLE
Zaizhong Zhang, Yang He, Xiaohuang Tu, Sheng Huang, Zhuo Chen, Lie Wang, Jingxiang Song
BACKGROUND: Aberrant DNA methylation patterns play a major role in tumorigenesis and the effects of nutrients, especially folate in the diet, on methylation changes is of great importance in colorectal cancer (CRC). Folate deficiency would disrupt methylation patterns; however, its exact effects on DNA methylation patterns in CRC are unclear. This study was performed to gain insight into the methylation changes induced by folate deficiency and the putative role of methylation pattern diversities of related genes in the clinical outcome of CRC...
April 1, 2017: Clinical Laboratory
https://read.qxmd.com/read/28383037/five-lipoxygenase-hypomethylation-mediates-the-homocysteine-effect-on-alzheimer-s-phenotype
#33
JOURNAL ARTICLE
Jian-Guo Li, Carlos Barrero, Salim Merali, Domenico Praticò
Environmental and genetic risk factors are implicated in the pathogenesis of Alzheimer's disease (AD). However, how they interact and influence its pathogenesis remains to be investigated. High level of homocysteine (Hcy) is an AD risk factor and associates with an up-regulation of the ALOX5 gene. In the current paper we investigated whether this activation is responsible for the Hcy effect on the AD phenotype and the mechanisms involved. Triple transgenic mice were randomized to receive regular chow diet, a diet deficient in folate and B vitamins (Diet), which results in high Hcy, or the Diet plus zileuton, a specific ALOX5 inhibitor, for 7 months...
April 6, 2017: Scientific Reports
https://read.qxmd.com/read/28369960/chronic-alcohol-exposure-differentially-alters-one-carbon-metabolism-in-rat-liver-and-brain
#34
JOURNAL ARTICLE
James Auta, Huaibo Zhang, Subhash C Pandey, Alessandro Guidotti
BACKGROUND: Epigenetic mechanisms such as DNA methylation play an important role in regulating the pathophysiology of alcoholism. Chronic alcohol exposure leads to behavioral changes as well as decreased expression of genes associated with synaptic plasticity. In the liver, it has been documented that chronic alcohol exposure impairs methionine synthase (Ms) activity leading to a decrease in S-adenosyl methionine/S-adenosyl homocysteine (SAM/SAH) ratio which results in DNA hypomethylation; however, it is not known whether similar alterations of SAM and SAH levels are also produced in brain...
June 2017: Alcoholism, Clinical and Experimental Research
https://read.qxmd.com/read/28239993/maternal-betaine-supplementation-in-rats-induces-intergenerational-changes-in-hepatic-igf-1-expression-and-dna-methylation
#35
JOURNAL ARTICLE
Nannan Zhao, Shu Yang, Yun Hu, Haibo Dong, Ruqian Zhao
SCOPE: Betaine is widely used in animal nutrition to promote growth. Here, we aimed to investigate whether maternal betaine supplementation during pregnancy can exert multigenerational effects on growth across two generations and the possible epigenetic modifications associated to such effects. METHODS AND RESULTS: In this study, 3-month-old female Sprague-Dawley rats were fed diet supplemented with 1% betaine throughout the pregnancy and lactation. Betaine-supplemented dams produced bigger litter but smaller F1 pups at birth and weaning...
August 2017: Molecular Nutrition & Food Research
https://read.qxmd.com/read/28179424/altered-methylation-of-specific-dna-loci-in-the-liver-of-bhmt-null-mice-results-in-repression-of-iqgap2-and-f2rl2-and-is-associated-with-development-of-preneoplastic-foci
#36
JOURNAL ARTICLE
Daniel S Lupu, Luz D Orozco, Ying Wang, John M Cullen, Matteo Pellegrini, Steven H Zeisel
Folate B12 -dependent remethylation of homocysteine is important, but less is understood about the importance of the alternative betaine-dependent methylation pathway-catalyzed by betaine-homocysteine methyltransferase (BHMT)-for establishing and maintaining adequate DNA methylation across the genome. We studied C57Bl/6J Bhmt (betaine-homocysteine methyltransferase)-null mice at age 4, 12, 24, and 52 wk ( N = 8) and observed elevation of S -adenosylhomocysteine concentrations and development of preneoplastic foci in the liver (increased placental glutathione S -transferase and cytokeratin 8-18 activity; starting at 12 wk)...
May 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/27992360/chronic-kidney-disease-induces-inflammatory-cd40-monocyte-differentiation-via-homocysteine-elevation-and-dna-hypomethylation
#37
JOURNAL ARTICLE
Jiyeon Yang, Pu Fang, Daohai Yu, Lixiao Zhang, Daqing Zhang, Xiaohua Jiang, William Y Yang, Teodoro Bottiglieri, Satya P Kunapuli, Jun Yu, Eric T Choi, Yong Ji, Xiaofeng Yang, Hong Wang
RATIONALE: Patients with chronic kidney disease (CKD) develop hyperhomocysteinemia and have a higher cardiovascular mortality than those without hyperhomocysteinemia by 10-fold. OBJECTIVE: We investigated monocyte differentiation in human CKD and cardiovascular disease (CVD). METHODS AND RESULTS: We identified CD40 as a CKD-related monocyte activation gene using CKD-monocyte -mRNA array analysis and classified CD40 monocyte (CD40+ CD14+ ) as a stronger inflammatory subset than the intermediate monocyte (CD14++ CD16+ ) subset...
November 11, 2016: Circulation Research
https://read.qxmd.com/read/27659321/maternal-mthfr-polymorphism-677-c-t-and-risk-of-down-s-syndrome-child-meta-analysis
#38
JOURNAL ARTICLE
Amandeep Kaur, Anupam Kaur
Methylenetetrahydrofolate reductase (MTHFR) is the most important gene that participates in folate metabolism. Presence of valine instead of alanine at position 677 and elevated levels of homocystein causes DNA hypomethylation which in turn favours nondisjunction. In this study, we conducted a meta-analysis to establish link between maternal single-nucleotide polymorphism (SNP) and birth of Down's syndrome (DS) child. A total of 37 case-control studies were selected for analysis including our own, in which we investigated 110 cases and 111 control mothers...
September 2016: Journal of Genetics
https://read.qxmd.com/read/27509954/methyl-donor-status-influences-dnmt-expression-and-global-dna-methylation-in-cervical-cancer-cells
#39
JOURNAL ARTICLE
Natwadee Poomipark, Janet E Flatley, Marilyn H Hill, Barbara Mangnall, Elnaz Azar, Peter Grabowski, Hilary J Powers
BACKGROUND: Methyl donor status influences DNA stability and DNA methylation although little is known about effects on DNA methyltransferases. The aim of this study was to determine whether methyldonor status influences DNA methyltransferase (Dnmt) gene expression in cervical cancer cells, and if so, whether there are associated effects on global DNA methylation. MATERIALS AND METHODS: The human cervical cancer cell line, C4 II, was grown in complete medium and medium depleted of folate (FM+) and folate and methionine (FM)...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://read.qxmd.com/read/27221153/fabp4-mediated-homocysteine-induced-cholesterol-accumulation-in-thp-1-monocyte-derived-macrophages-and-the-potential-epigenetic-mechanism
#40
JOURNAL ARTICLE
Yideng Jiang, Shengchao Ma, Huiping Zhang, Xiaoling Yang, Guan Jun Lu, Hui Zhang, Yangyang He, Fanqi Kong, Anning Yang, Hua Xu, Minghao Zhang, Yun Jiao, Guizhong Li, Jun Cao, Yuexia Jia, Shaoju Jin, Jun Wei, Yingkang Shi
Hyperhomocysteinemia (HHcy) is an independent risk factor for the development of atherosclerosis (AS), according to overwhelming number of clinical and epidemiological studies. However, the underlying pathogenic molecular mechanisms by which HHcy promotes AS remain to be fully elucidated. Fatty acid binding protein 4 (FABP4) has been shown to be important in macrophage cholesterol trafficking. The objective of the present study was to determine whether homocysteine (Hcy) accelerates AS through regulating FABP4, and then mediates cholesterol accumulation in macrophages...
July 2016: Molecular Medicine Reports
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