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Eric Dumonteil, Maria-Jesus Ramirez-Sierra, Silvia Pérez-Carrillo, Christian Teh-Poot, Claudia Herrera, Sébastien Gourbière, Etienne Waleckx
Trypanosoma cruzi is the agent of Chagas disease, transmitted by hematophagous triatomine vectors. Establishing transmission cycles is key to understand the epidemiology of the disease, but integrative assessments of ecological interactions shaping parasite transmission are still limited. Current approaches also lack sensitivity to assess the full extent of this ecological diversity. Here we developed a metabarcoding approach based on next-generation sequencing to identify triatomine gut microbiome, vertebrate feeding hosts, and parasite diversity and their potential interactions...
March 7, 2018: Scientific Reports
Leandro José Ramos, Janis Lunier de Souza, Cláudio Rodrigues de Souza, Jader de Oliveira, João Aristeu da Rosa, Luis Marcelo Aranha Camargo, Renildo Moura da Cunha, Dionatas Ulises de Oliveira Meneguetti
INTRODUCTION: The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time. METHODS: Triatoma sordida specimens were collected from a Gallus gallus nest in a peridomiciliary area of Senador Guiomard municipality in the state of Acre. RESULTS: The number of triatomine species in Acre increased from six to seven with this first report of T. sordida in the Brazilian Western Amazon. CONCLUSIONS: The occurrence of T...
January 2018: Revista da Sociedade Brasileira de Medicina Tropical
M Constanza Mannino, M Patricia Juárez, Nicolás Pedrini
The chemical control of Triatoma infestans, the major Chagas disease vector in southern South America, has been threatened in the last years by the emergence of pyrethroid-resistant bug populations. As an alternative approach, the efficacy of the entomopathogenic fungus Beauveria bassiana to control T. infestans populations (regardless their pyrethroid susceptibility) has been demonstrated. Growing research efforts on the interaction between T. infestans and B. bassiana by molecular, ecological, biochemical and behavioral traits has allowed framing such interaction as an evolutionary arms race...
February 26, 2018: Infection, Genetics and Evolution
Mónica D Germano, María I Picollo
Triatoma infestans Klug (Hemiptera: Reduviidae) is the main vector of Chagas disease in Latin America. This insect has been controlled with pyrethroids since the 1980s, although the emergence of resistance to deltamethrin has decreased control success in some areas of the Gran Chaco ecoregion. The response of T. infestans to deltamethrin was evaluated per developmental stage. In addition, we evaluated the possible stage-dependent expression of deltamethrin resistance. The bioassays were conducted by topical application of the insecticide in acetone...
February 20, 2018: Journal of Medical Entomology
Jessica C Nevoa, Maria T Mendes, Marcos V da Silva, Siomar C Soares, Carlo J F Oliveira, José M C Ribeiro
Triatomines are hematophagous arthropod vectors of Trypanosoma cruzi, the causative agent of Chagas Disease. Panstrongylus lignarius, also known as Panstrongylus herreri, is considered one of the most versatile triatomines because it can parasitize different hosts, it is found in different habitats and countries, it has sylvatic, peridomestic and domestic behavior and it is a very important vector of Chagas disease, especially in Peru. Molecules produced and secreted by salivary glands and fat body are considered of important adaptational value for triatomines because, among other functions, they subvert the host haemostatic, inflammatory and immune systems and detoxify or protect them against environmental aggressors...
February 20, 2018: PLoS Neglected Tropical Diseases
Jorge Espinoza Echeverria, Marinely B Bustamante Gomez, Grasielle Caldas D Ávila Pessoa, Mirko Rojas Cortez, Antonio Nogales Rodriguez, Liléia Gonçalves Diotaiuti
BACKGROUND: Chemical control with pyrethroid insecticides has been effective in reducing endemic areas of distribution of Triatoma infestans in the Southern Cone, as well as Bolivia; this had considerably reduced the infestation of households in a large part of the territory. Nowadays, areas such as the Chaco and the Inter-Andean Valleys are regions where the reach of vector control strategies is becoming limited, and infestations of insecticide-treated households are reported more often...
February 17, 2018: Parasites & Vectors
Irving J May-Concha, Leopoldo C Cruz-López, Julio C Rojas, Janine M Ramsey
BACKGROUND: The Triatoma phyllosoma complex of Trypanosoma cruzi vectors (Triatominae: Reduviidae) is distributed in both Neotropical and Nearctic bioregions of Mexico. METHODS: Volatile organic compounds emitted by disturbed Triatoma longipennis, Triatoma pallidipennis and Triatoma phyllosoma, and from their Brindley's and metasternal glands, were identified using solid-phase microextraction coupled with gas chromatography-mass spectrometry. RESULTS: Disturbed bugs and the metasternal glands from T...
February 17, 2018: Parasites & Vectors
Ana E Gutiérrez-Cabrera, Wesley F Zandberg, Edgar Zenteno, Mario H Rodríguez, Bertha Espinoza, Carl Lowenberger
Trypanosoma cruzi, the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage. Many studies suggest that the parasite binds to midgut-specific glycans. We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions. In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes...
February 15, 2018: Insect Science
P Lobbia, J Calcagno, G Mougabure-Cueto
Pyrethroid resistance has been detected in Triatoma infestans (Klug) (Hemiptera: Reduviidae) specimens from different areas of Argentina and Bolivia. Genes conferring resistance can have a pleiotropic effect with epidemiological and evolutionary consequences. This research studied excretion/defecation patterns in deltamethrin-resistant T. infestans in order to elucidate its biological performance, adaptive consequences and role in the transmission of Chagas' disease. One deltamethrin-susceptible strain and two deltamethrin-resistant strains were used...
February 12, 2018: Medical and Veterinary Entomology
Alexander Vargas, Juliane Maria Alves Siqueira Malta, Veruska Maia da Costa, Leandro Del Grande Cláudio, Renato Vieira Alves, Gilmar da Silva Cordeiro, Lúcia Maria Abrantes Aguiar, Jadher Percio
The aim of this article was to confirm and describe an outbreak of acute Chagas disease involving oral transmission in the western region of Rio Grande do Norte State, Brazil. This was a descriptive case series study in which the data sources were medical records and interviews with suspected cases from September 16 to November 19, 2015. An entomological investigation was conducted in the probable sites of infection for acute Chagas disease cases. Eighteen cases of acute Chagas disease were confirmed in residents of four municipalities (counties) in Rio Grande do Norte State...
February 5, 2018: Cadernos de Saúde Pública
Romina V Piccinali, Michael W Gaunt, Ricardo E Gürtler
Prevention of vector-borne transmission of Chagas disease mainly relies on residual insecticide spraying. Despite significant success at a regional scale, house infestation with Triatoma infestans (Klug) (Hemiptera: Reduviidae) still persists in the Gran Chaco ecoregion. One key aspect is the identification of the sources of reinfestant triatomines. After detecting fine-scale genetic structure in two rural villages of Pampa del Indio, Argentine Chaco, we tested hypotheses on the putative origins of the triatomines collected at 4, 8, and 12 mo after insecticide house spraying...
January 27, 2018: Journal of Medical Entomology
Carla G Grosso, María M Stroppa, Gonzalo M Varela, Beatriz A García
Pyrethroid resistance has been detected in Triatoma infestans (Hemiptera: Reduviidae), which was atributed to target site insensitivity and increased oxidative metabolism of the insecticide by cytochrome P450s. Nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) plays an essential role in transferring electrons from NADPH to the P450-substrate complex. In this study, the full length CPR cDNA of T. infestans was isolated and gene expression was determined by quantitative polymerase chain reaction...
January 22, 2018: American Journal of Tropical Medicine and Hygiene
Federico Alonso Zumaya-Estrada, Jesús Martínez-Barnetche, Andrés Lavore, Rolando Rivera-Pomar, Mario Henry Rodríguez
BACKGROUND: Insects operate complex humoral and cellular immune strategies to fend against invading microorganisms. The majority of these have been characterized in Drosophila and other dipterans. Information on hemipterans, including Triatominae vectors of Chagas disease remains incomplete and fractionated. RESULTS: We identified putative immune-related homologs of three Triatominae vectors of Chagas disease, Triatoma pallidipennis, T. dimidiata and T. infestans (TTTs), using comparative transcriptomics based on established immune response gene references, in conjunction with the predicted proteomes of Rhodnius prolixus, Cimex lecticularis and Acyrthosiphon pisum hemimetabolous...
January 22, 2018: Parasites & Vectors
Natalia Martín-González, Sofía Guérin, Aritz Durana, Gerardo Martí, Diego M Guérin, Pedro Jose J De Pablo Gomez
Even though viruses evolve mainly in liquid milieu, their horizontal transmission routes often include episodes of dry environment. Along their life cycle, some insect viruses, such as viruses from Dicistroviridae family, withstand dehydrated conditions with yet unknown consequences in their structural stability. Here we use Atomic Force Microscopy to monitor the structural changes of viral particles of Triatoma virus (TrV) after desiccation. Our results demonstrate that TrV capsids preserve their genome inside, conserving their height after exposure to dehydrating conditions, that is in stark contrast with other viruses that expel their genome when desiccated...
January 19, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
Thaís Tâmara Castro Minuzzi-Souza, Nadjar Nitz, César Augusto Cuba Cuba, Marcelo Santalucia, Monique Knox, Luciana Hagström, Camilla Bernardes Furtado, Tamires Emanuele Vital, Marcos Takashi Obara, Mariana Machado Hecht, Rodrigo Gurgel-Gonçalves
INTRODUCTION: Chagas disease surveillance requires current knowledge on synanthropic triatomines. We analyzed the occurrence and Trypanosoma cruzi infection rates of triatomine bugs in central Brazil, during 2012-2014. METHODS: Triatomines were collected inside or around houses, and T. cruzi infection was determined by optical microscopy and conventional/quantitative polymerase chain reaction. RESULTS: Of the 2706 triatomines collected, Triatoma sordida was the most frequent species in Goiás State, whereas Panstrongylus megistus predominated in the Federal District...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
Edson Santos Dantas, Rodrigo Gurgel-Gonçalves, Daniel Antunes Maciel Villela, Fernando Araújo Monteiro, Rafael Maciel-de-Freitas
BACKGROUND: Chagas disease, or American trypanosomiasis, is an important neglected tropical illness caused by the flagellate protozoan Trypanosoma cruzi, which is primarily transmitted to humans by hematophagous insects of the subfamily Triatominae. Although knowledge on triatomine movement capabilities at the micro-geographical scale is of fundamental importance concerning the development of effective vector control strategies, it remains a poorly understood subject. Furthermore, survival rates and size estimates of natural populations are important topics to consider when evaluating transmission intensity...
January 5, 2018: Parasites & Vectors
Paula Beatriz Santiago, Carla Nunes de Araújo, Sébastien Charneau, Izabela Marques Dourado Bastos, Teresa Cristina F Assumpção, Rayner Myr Lauterjung Queiroz, Yanna Reis Praça, Thuany de Moura Cordeiro, Carlos Henrique Saraiva Garcia, Ionizete Garcia da Silva, Tainá Raiol, Flávia Nader Motta, João Victor de Araújo Oliveira, Marcelo Valle de Sousa, José Marcos C Ribeiro, Jaime Martins de Santana
Triatoma dimidiata, a Chagas disease vector widely distributed along Central America, has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas fed human blood. Exploring the salivary compounds that overcome host haemostatic and immune responses is of great scientific interest. Here, we provide a deeper insight into its salivary gland molecules. We used high-throughput RNA sequencing to examine in depth the T. dimidiata salivary gland transcriptome...
December 27, 2017: Journal of Proteomics
Kaio Cesar Chaboli Alevi, Heloisa Pinotti, Renato Freitas de Araújo, Maria Tercília Vilela de Azeredo Oliveira, João Aristeu da Rosa, Vagner José Mendonça
Six to seven million people worldwide are estimated to be infected with Trypanosoma cruzi, the etiologic agent of Chagas disease transmitted mainly by triatomines. Triatoma bahiensis was recently collected in the Brazilian state of Bahia and revalidated using different approaches. The main criterion used to define a "good" biological species is reproductive isolation, so we evaluated the cytogenetics of first-generation (F1) hybrids resulting from the experimental cross between T. bahiensis females and Triatoma lenti males to possibly characterize the postzygotic isolation associated with the hybrid breakdown...
December 18, 2017: American Journal of Tropical Medicine and Hygiene
María M Stroppa, Ignacio Gimenez, Beatriz A García
To contribute to a better understanding of the molecular bases of the circadian biological rhythms in Chagas disease vectors, in this work we identified functional domains in the sequences of the clock protein PERIOD (PER) in Rhodnius prolixus and Triatoma infestans and analyzed the expression of the PER gene at mRNA level in T. infestans. The PER protein sequences comparison among these species and those from other insects revealed that the most similar regions are the PAS domains and the most variable is the COOH-terminal...
December 18, 2017: American Journal of Tropical Medicine and Hygiene
Silvia A Justi, Sara Cahan, Lori Stevens, Carlota Monroy, Raquel Lima, Patricia L Dorn
To date, the phylogeny of Triatoma dimidiata sensu lato (s. l.) (Hemiptera: Reduviidae: Triatominae), the epidemiologically most important Chagas disease vector in Central America and a secondary vector in Mexico and northern South America, has only been investigated by one multi-copy nuclear gene (Internal Transcribed Spacer - 2) and a few mitochondrial genes. We examined 450 specimens sampled across most of its native range from Mexico to Ecuador using reduced representation next-generation sequencing encompassing over 16,000 single nucleotide polymorphisms (SNPs)...
December 14, 2017: Molecular Phylogenetics and Evolution
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