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https://www.readbyqxmd.com/read/29349579/malaria-infectivity-of-xanthurenic-acid-deficient-anopheline-mosquitoes-produced-by-talen-mediated-targeted-mutagenesis
#1
Daisuke S Yamamoto, Megumi Sumitani, Masatsugu Hatakeyama, Hiroyuki Matsuoka
Anopheline mosquitoes are major vectors of malaria parasites. When the gametocytes of the malaria parasite are transferred from a vertebrate to mosquitoes, they differentiate into gametes, and are fertilized in the midguts of mosquitoes. Xanthurenic acid (XA), a waste product of the ommochrome synthesis pathway, has been shown to induce exflagellation during microgametogenesis in vitro; however, it currently remains unclear whether endogenous XA affects the infectivity of anopheline mosquitoes to malaria parasites in vivo due to the lack of appropriate experimental systems such as a XA-deficient line...
January 18, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29348555/novel-immunoinformatics-approaches-to-design-multi-epitope-subunit-vaccine-for-malaria-by-investigating-anopheles-salivary-protein
#2
Rajan Kumar Pandey, Tarun Kumar Bhatt, Vijay Kumar Prajapati
Malaria fever has been pervasive for quite a while in tropical developing regions causing high morbidity and mortality. The causal organism is a protozoan parasite of genus Plasmodium which spreads to the human host by the bite of hitherto infected female Anopheles mosquito. In the course of biting, a salivary protein of Anopheles helps in blood feeding behavior and having the ability to elicit the host immune response. This study represents a series of immunoinformatics approaches to design multi-epitope subunit vaccine using Anopheles mosquito salivary proteins...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29346647/resistance-to-selected-pyrethroid-insecticides-in-the-malaria-mosquito-anopheles-stephensi-diptera-muscidae-from-punjab-pakistan
#3
Hafiz Azhar Ali Khan, Waseem Akram, Sumi Lee
Pyrethroids are commonly used insecticides in malaria control programs; however, insecticide resistance limits the benefits gained by using these insecticides. In the present study, we assessed the resistance status for different pyrethroids of the malaria mosquito vector, Anopheles stephensi Liston (Diptera: Muscidae), in the Punjab province, Pakistan. Bioassays were conducted using diagnostic doses following standard World Health Organization protocols: 0.05% λ-cyhalothrin, 0.75% permethrin, 0.15% cyfluthrin, 0...
January 16, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29344968/a-novel-nested-polymerase-chain-reaction-assay-targeting-plasmodium-mitochondrial-dna-in-field-collected-anopheles-mosquitoes
#4
M Calzetta, E Perugini, G Seixas, C A Sousa, W M Guelbeogo, N Sagnon, A Della Torre, J Pinto, M Pombi, E Mancini
Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium circumsporozoite protein (CSP-ELISA). However, because of the relatively high costs associated with equipment and reagents, RT-PCRs are mostly used to assess the outcomes of experimental infections in the frame of research experiments, rather than in routine monitoring of mosquito infection in the field...
January 18, 2018: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/29343279/spatial-distribution-and-habitat-characterization-of-mosquito-species-during-the-dry-season-along-the-mara-river-and-its-tributaries-in-kenya-and-tanzania
#5
Gabriel O Dida, Douglas N Anyona, Paul O Abuom, Daniel Akoko, Samson O Adoka, Ally-Said Matano, Philip O Owuor, Collins Ouma
BACKGROUND: Vector-borne diseases are increasingly becoming a major health problem among communities living along the major rivers of Africa. Although larger water bodies such as lakes and dams have been extensively researched, rivers and their tributaries have largely been ignored. This study sought to establish the spatial distribution of mosquito species during the dry season and further characterize their habitats along the Mara River and its tributaries. METHODS: In this cross-sectional survey, mosquito larvae were sampled along the Mara River, its two perennial tributaries (Amala and Nyangores), drying streams, and adjacent aquatic habitats (e...
January 18, 2018: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29338719/preventing-malaria-in-the-peruvian-amazon-a-qualitative-study-in-iquitos-peru
#6
Ian Newell, Connie Wiskin, James Anthoney, Graciela Meza, Gilles de Wildt
BACKGROUND: In Peru, despite decades of concerted control efforts, malaria remains a significant public health burden. Peru has recently exhibited a dramatic rise in malaria incidence, impeding South America's progress towards malaria elimination. The Amazon basin, in particular the Loreto region of Peru, has been identified as a target for the implementation of intensified control strategies, aiming for elimination. No research has addressed why vector control strategies in Loreto have had limited impact in the past, despite vector control elsewhere being highly effective in reducing malaria transmission...
January 16, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29335015/cryptic-diversity-in-an-atlantic-forest-malaria-vector-from-the-mountains-of-south-east-brazil
#7
Guilherme de Rezende Dias, Thais Tenorio Soares Fujii, Bernardo Fernandes Fogel, Ricardo Lourenço-de-Oliveira, Teresa Fernandes Silva-do-Nascimento, André Nóbrega Pitaluga, Carlos José Carvalho-Pinto, Antonio Bernardo Carvalho, Alexandre Afrânio Peixoto, Luísa Damazio Pitaluga Rona
BACKGROUND: Anopheles (Kerteszia) cruzii is the primary vector of human and simian malarias in Brazilian regions covered by the Atlantic Rainforest. Previous studies found that An. cruzii presents high levels of behavioural, chromosomal and molecular polymorphisms, which led to the hypothesis that it may be a complex of cryptic species. Here, An. cruzii specimens were collected in five sites in South-East Brazil located at different altitudes on the inner and coastal slopes of two mountain ranges covered by Atlantic Rainforest, known as Serra do Mar and Serra da Mantiqueria...
January 15, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29334982/genome-wide-identification-characterization-and-classification-of-ionotropic-glutamate-receptor-genes-iglurs-in-the-malaria-vector-anopheles-sinensis-diptera-culicidae
#8
Ting-Ting Wang, Feng-Ling Si, Zheng-Bo He, Bin Chen
BACKGROUND: Ionotropic glutamate receptors (iGluRs) are conserved ligand-gated ion channel receptors, and ionotropic receptors (IRs) were revealed as a new family of iGluRs. Their subdivision was unsettled, and their characteristics are little known. Anopheles sinensis is a major malaria vector in eastern Asia, and its genome was recently well sequenced and annotated. METHODS: We identified iGluR genes in the An. sinensis genome, analyzed their characteristics including gene structure, genome distribution, domains and specific sites by bioinformatic methods, and deduced phylogenetic relationships of all iGluRs in An...
January 15, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29330677/evaluating-efficacy-of-landsat-derived-environmental-covariates-for-predicting-malaria-distribution-in-rural-villages-of-vhembe-district-south-africa
#9
Oupa E Malahlela, Jane M Olwoch, Clement Adjorlolo
Malaria in South Africa is still a problem despite existing efforts to eradicate the disease. In the Vhembe District Municipality, malaria prevalence is still high, with a mean incidence rate of 328.2 per 100,0000 persons/year. This study aimed at evaluating environmental covariates, such as vegetation moisture and vegetation greenness, associated with malaria vector distribution for better predictability towards rapid and efficient disease management and control. The 2005 malaria incidence data combined with Landsat 5 ETM were used in this study...
January 12, 2018: EcoHealth
https://www.readbyqxmd.com/read/29329539/state-dependent-domicile-leaving-rates-in-anopheles-gambiae
#10
Simon P W Zappia, Alex M Chubaty, Bernard D Roitberg
BACKGROUND: Transmission of Plasmodium greatly depends on the foraging behaviour of its mosquito vector (Anopheles spp.). The accessibility of blood hosts and availability of plant sugar (i.e., nectar) sources, together with mosquito energy state, have been shown to modulate blood feeding (and thus biting rates) of anopheline mosquitoes. In this study, the influence of mosquito starvation status and availability of nectar on the decision of female Anopheles gambiae mosquitoes to leave a bed net-protected blood host was examined...
January 12, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29327416/comparative-proteomics-studies-of-insect-cuticle-by-tandem-mass-spectrometry-application-of-a-novel-proteomics-approach-to-the-pea-aphid-cuticular-proteins
#11
Masson Victor, Arafah Karim, Voisin Sébastien, Bulet Philippe
The exoskeleton of arthropods, called cuticle, is a biological composite material consisting principally of N-acetylglucosamine polymer embedded in cuticular proteins (CPs). The CPs have been studied and characterized by mass spectrometry in several cuticular structures and in many arthropods. Such analyses were usually carried out by protein extraction using SDS detergent followed by electrophoresis, allowing detection and identification of a large number of CPs. To build a repertoire of cuticular structures from Bombyx mori, Apis mellifera and Anopheles gambiae we avoided the use of SDS and electrophoresis...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29325101/fauna-ecological-characteristics-and-checklist-of-the-mosquitoes-in-mazandaran-province-northern-iran
#12
Seyed Hassan Nikookar, Mahmoud Fazeli-Dinan, Shahyad Azari-Hamidian, Seyed Nouraddin Mousavi Nasab, Mohsen Aarabi, Seyyed Payman Ziapour, Ahmadali Enayati, Janet Hemingway
Mosquitoes are important vectors of human and animal diseases. This study updates current knowledge on fauna, dominance, and distribution of mosquitoes in Mazandaran Province, Northern Iran, to inform disease control effort. Larval collections, using standard dippers or droppers, and adult collections, using total catches, shelter pits, CDC light traps, and human landing catches, were performed monthly in 30 villages across 16 counties, from May to December 2014. Ovitraps, baited with hay infusion as oviposition attractants or stimulants for Aedes (Stegomyia) mosquitoes, were installed in each village and inspected weekly for eggs...
January 6, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29324038/the-evolution-and-metamorphosis-of-arthropod-proteomics-and-genomics
#13
Judith H Willis
This article presents an overview of the development of techniques for analyzing cuticular proteins (CPs), their transcripts, and their genes over the past 50 years based primarily on experience in the laboratory of J.H. Willis. It emphasizes changes in the kind of data that can be gathered and how such data provided insights into the molecular underpinnings of insect metamorphosis and cuticle structure. It describes the techniques that allowed visualization of the location of CPs at both the anatomical and intracuticular levels and measurement of the appearance and deployment of transcripts from CP genes as well as what was learned from genomic and transcriptomic data...
January 7, 2018: Annual Review of Entomology
https://www.readbyqxmd.com/read/29322852/anion-inhibition-studies-of-a-beta-carbonic-anhydrase-from-the-malaria-mosquito-anopheles-gambiae
#14
Daniela Vullo, Leo Syrjänen, Marianne Kuuslahti, Seppo Parkkila, Claudiu T Supuran
An anion inhibition study of the β-class carbonic anhydrase, AgaCA, from the malaria mosquito Anopheles gambiae is reported. A series of simple as well as complex inorganic anions, and small molecules known to interact with CAs were included in the study. Bromide, iodide, bisulphite, perchlorate, perrhenate, perruthenate, and peroxydisulphate were ineffective AgaCA inhibitors, with KIs > 200 mM. Fluoride, chloride, cyanate, thiocyanate, cyanide, bicarbonate, carbonate, nitrite, nitrate, sulphate, stannate, selenate, tellurate, diphosphate, divanadate, tetraborate, selenocyanide, and trithiocarbonate showed KIs in the range of 1...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/29322058/gas-chromatography-gc-mass-analysis-and-bioactivity-of-essential-oil-from-aerial-parts-of-ferulago-trifida-antimicrobial-antioxidant-ache-inhibitory-general-toxicity-mtt-assay-and-larvicidal-activities
#15
Saeed Tavakoli, Hassan Vatandoost, Reza Zeidabadinezhad, Reza Hajiaghaee, Abbas Hadjiakhoondi, Mohammad Reza Abai, Narguess Yassa
Background: We aimed to investigate different biological properties of aerial parts essential oil of Ferulago trifida Boiss and larvicidal activity of its volatile oils from all parts of plant. Methods: Essential oil was prepared by steam distillation and analyzed by Gas chromatography and GC/Mass. Antioxidant, antimicrobial, cytotoxic effects and AChE inhibitory of the oil were investigated using DPPH, disk diffusion method, MTT assay and Ellman methods. Larvicidal activity of F...
September 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29322052/composition-of-anopheles-species-collected-from-selected-malarious-areas-of-afghanistan-and-iran
#16
Helen Hoosh-Deghati, Navid Dinparast-Djadid, Vahideh Moin-Vaziri, Hoda Atta, Abbas Ali Raz, Seyyed Javad Seyyed-Tabaei, Naseh Maleki-Ravasan, Hamzeh Alipour, Sedigheh Zakeri, Eznollah Azar-Gashb
Background: Malarious areas in Iran are close to Afghanistan and Pakistan that urge the researchers to extend their knowledge on malaria epidemiology to the neighboring countries as well. Vectorial capacity differs at species or even at population level, the first essential step is accurate identification of vectors. This study aimed to identify Anopheles species composition in selected malarious areas of Afghanistan and Iran, providing further applied data for other research in two countries...
September 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29321052/asgdb-a-specialised-genomic-resource-for-interpreting-anopheles-sinensis-insecticide%C3%A2-resistance
#17
Dan Zhou, Yang Xu, Cheng Zhang, Meng-Xue Hu, Yun Huang, Yan Sun, Lei Ma, Bo Shen, Chang-Liang Zhu
BACKGROUND: Anopheles sinensis is an important malaria vector in Southeast Asia. The widespread emergence of insecticide resistance in this mosquito species poses a serious threat to the efficacy of malaria control measures, particularly in China. Recently, the whole-genome sequencing and de novo assembly of An. sinensis (China strain) has been finished. A series of insecticide-resistant studies in An. sinensis have also been reported. There is a growing need to integrate these valuable data to provide a comprehensive database for further studies on insecticide-resistant management of An...
January 10, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29316917/an-observational-analysis-of-the-impact-of-indoor-residual-spraying-with-non-pyrethroid-insecticides-on-the-incidence-of-malaria-in-s%C3%A3-gou-region-mali-2012-2015
#18
Joseph Wagman, Christelle Gogue, Kenzie Tynuv, Jules Mihigo, Elie Bankineza, Mamadou Bah, Diadier Diallo, Andrew Saibu, Jason H Richardson, Diakalkia Kone, Seydou Fomba, Jeff Bernson, Richard Steketee, Laurence Slutsker, Molly Robertson
BACKGROUND: Ségou Region in Central Mali is an area of high malaria burden with seasonal transmission, high access to and use of long-lasting insecticidal nets (LLINs), and resistance to pyrethroids and DDT well documented in Anopheles gambiae s.l. (the principal vector of malaria in Mali). Ségou has recently received indoor residual spraying (IRS) supported by Mali's collaboration with the US President's Malaria Initiative/Africa Indoor Residual Spraying programme. From 2012 to 2015, two different non-pyrethroid insecticides: bendiocarb in 2012 and 2013 and pirimiphos-methyl in 2014 and 2015, were used for IRS in two districts...
January 10, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29316140/a-male-gametocyte-osmiophilic-body-and-microgamete-surface-protein-of-the-rodent-malaria-parasite-plasmodium-yoelii-pymigs-plays-a-critical-role-in-male-osmiophilic-body-formation-and-exflagellation
#19
Mayumi Tachibana, Tomoko Ishino, Eizo Takashima, Takafumi Tsuboi, Motomi Torii
Anopheles mosquitoes transmit Plasmodium parasites of mammals, including the species that cause malaria in humans. Malaria pathology is caused by rapid multiplication of parasites in asexual intraerythrocytic cycles. Sexual stage parasites are also produced during the intraerythrocytic cycle, and are ingested by the mosquito, initiating gametogenesis and subsequent sporogonic stage development. Here, we present a Plasmodium protein, termed microgamete surface protein (MiGS), which has an important role in male gametocyte osmiophilic body (MOB) formation and microgamete function...
January 5, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29310704/mapping-insecticide-resistance-in-anopheles-gambiae-s-l-from-c%C3%A3-te-d-ivoire
#20
Soromane Camara, Alphonsine A Koffi, Ludovic P Ahoua Alou, Kouakou Koffi, Jean-Paul K Kabran, Aboubacar Koné, Mathieu F Koffi, Raphaël N'Guessan, Cédric Pennetier
BACKGROUND: Insecticide resistance in malaria vectors is an increasing threat to vector control tools currently deployed in endemic countries. Resistance management must be an integral part of National Malaria Control Programmes' (NMCPs) next strategic plans to alleviate the risk of control failure. This obviously will require a clear database on insecticide resistance to support the development of such a plan. The present work gathers original data on insecticide resistance between 2009 and 2015 across Côte d'Ivoire in West Africa...
January 8, 2018: Parasites & Vectors
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