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https://www.readbyqxmd.com/read/28760210/a-randomized-trial-of-a-1-hour-troponin-t-protocol-in-suspected-acute-coronary-syndromes-design-of-the-rapid-assessment-of-possible-acs-in-the-emergency-department-with-high-sensitivity-troponin-t-rapid-tnt-study
#1
RANDOMIZED CONTROLLED TRIAL
Cynthia Papendick, Andrew Blyth, Anil Seshadri, Michael J R Edmonds, Tom Briffa, Louise Cullen, Stephen Quinn, Jon Karnon, Anthony Chuang, Adam J Nelson, Matthew Horsfall, Erin Morton, Derek P Chew
BACKGROUND: Protocols incorporating high-sensitivity troponin to guide decision making in the disposition of patients with suspected acute coronary syndromes (ACS) in the emergency department have received a lot of attention. Traditionally, patients with chest pain have required long periods of observation in emergency department before being deemed safe for discharge. In an era of limited health service resources, a protocol that could discharge patients safely within an hour of presentation is extremely attractive...
August 2017: American Heart Journal
https://www.readbyqxmd.com/read/28741696/age-dependent-regulation-of-erf-vii-transcription-factor-activity-in-arabidopsis-thaliana
#2
Beatrice Giuntoli, Vinay Shukla, Federica Maggiorelli, Federico M Giorgi, Lara Lombardi, Pierdomenico Perata, Francesco Licausi
The group VII Ethylene Responsive Factors (ERF-VII) RAP2.2 and RAP2.12 have been mainly characterized with regards to their contribution as activators of fermentation in plants. However, transcriptional changes measured in conditions that stabilise these transcription factors exceed the mere activation of this biochemical pathway, implying additional roles performed by the ERF-VIIs in other processes. We evaluated gene expression in transgenic Arabidopsis lines expressing a stabilised form of RAP2.12, or hampered in ERF-VII activity, and identified genes affected by this transcriptional regulator and its homologs, including some involved in oxidative stress response, which are not universally induced under anaerobic conditions...
July 25, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28740232/p62-sqstm1-sequestosome-1-is-an-n-recognin-of-the-n-end-rule-pathway-which-modulates-autophagosome-biogenesis
#3
Hyunjoo Cha-Molstad, Ji Eun Yu, Zhiwei Feng, Su Hyun Lee, Jung Gi Kim, Peng Yang, Bitnara Han, Ki Woon Sung, Young Dong Yoo, Joonsung Hwang, Terry McGuire, Sang Mi Shim, Hyun Dong Song, Srinivasrao Ganipisetti, Nuozhou Wang, Jun Min Jang, Min Jae Lee, Seung Jun Kim, Kyung Ho Lee, Jin Tae Hong, Aaron Ciechanover, Inhee Mook-Jung, Kwang Pyo Kim, Xiang-Qun Xie, Yong Tae Kwon, Bo Yeon Kim
Macroautophagy mediates the selective degradation of proteins and non-proteinaceous cellular constituents. Here, we show that the N-end rule pathway modulates macroautophagy. In this mechanism, the autophagic adapter p62/SQSTM1/Sequestosome-1 is an N-recognin that binds type-1 and type-2 N-terminal degrons (N-degrons), including arginine (Nt-Arg). Both types of N-degrons bind its ZZ domain. By employing three-dimensional modeling, we developed synthetic ligands to p62 ZZ domain. The binding of Nt-Arg and synthetic ligands to ZZ domain facilitates disulfide bond-linked aggregation of p62 and p62 interaction with LC3, leading to the delivery of p62 and its cargoes to the autophagosome...
July 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28735697/disentangling-heterogeneity-of-childhood-disruptive-behavior-problems-into-dimensions-and-subgroups
#4
Koen Bolhuis, Gitta H Lubke, Jan van der Ende, Meike Bartels, Catharina E M van Beijsterveldt, Paul Lichtenstein, Henrik Larsson, Vincent W V Jaddoe, Steven A Kushner, Frank C Verhulst, Dorret I Boomsma, Henning Tiemeier
OBJECTIVE: Irritable and oppositional behaviors are increasingly considered as distinct dimensions of oppositional defiant disorder. However, few studies have explored this multidimensionality across the broader spectrum of disruptive behavior problems (DBPs). This study examined the presence of dimensions and distinct subgroups of childhood DBPs, and the cross-sectional and longitudinal associations between these dimensions. METHOD: Using factor mixture models (FMMs), the presence of dimensions and subgroups of DBPs was assessed in the Generation R Study at ages 6 (n = 6,209) and 10 (n = 4,724) years...
August 2017: Journal of the American Academy of Child and Adolescent Psychiatry
https://www.readbyqxmd.com/read/28652337/mine-conformational-dynamics-regulate-membrane-binding-mind-interaction-and-min-oscillation
#5
Kyung-Tae Park, Maria T Villar, Antonio Artigues, Joe Lutkenhaus
In Escherichia coli MinE induces MinC/MinD to oscillate between the ends of the cell, contributing to the precise placement of the Z ring at midcell. To do this, MinE undergoes a remarkable conformational change from a latent 6β-stranded form that diffuses in the cytoplasm to an active 4β-stranded form bound to the membrane and MinD. How this conformational switch occurs is not known. Here, using hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) we rule out a model in which the two forms are in rapid equilibrium...
July 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28633696/cross-border-collaboration-for-improved-tuberculosis-prevention-and-care-policies-tools-and-experiences
#6
M Dara, G Sulis, R Centis, L D'Ambrosio, G de Vries, P Douglas, D Garcia, N Jansen, E Zuroweste, G B Migliori
As tuberculosis (TB) spreads beyond borders with people movements, several interventions ensuring the continuity of care are essential, although difficult to put in place in the absence of well-defined agreements allowing data sharing and easy referral of patients to appropriate health facilities. This article first sets out general principles for cross-border collaboration and continuity of care. It then presents a series of case studies. Policies and practices on cross-border collaboration in selected low-incidence countries (Australia, Italy, Norway, The Netherlands, the United Kingdom and the United States) are described and critically appraised...
July 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28616536/nt-probnp-and-diastolic-left-ventricular-function-in-patients-with-marfan-syndrome
#7
Petra Gehle, Peter N Robinson, Frank Heinzel, Frank Edelmann, Mustafa Yigitbasi, Felix Berger, Volkmar Falk, Burkert Pieske, Ernst Wellnhofer
AIMS: Subclinical diastolic dysfuntion in patients with preclinical heart failure with preserved ejection fraction (HFpEF) has been demonstrated in patients with Marfan syndrome (MFS). We investigated the relationship between diastolic dysfunction and NT-proBNP levels in patients with MFS. METHODS AND RESULTS: NT-proBNP, C-reactive protein (CRP) and diastolic function were assessed in 217 patients with MFS (31 ± 16 y, 110 f. and in 339 patients referred for suspected MFS in whom the diagnosis was ruled out according to the Ghent nosology (30 ± 15 y, 154 f)...
September 2016: IJC Heart & Vasculature
https://www.readbyqxmd.com/read/28602583/coupled-caspase-and-n-end-rule-ligase-activities-allow-recognition-and-degradation-of-pluripotency-factor-lin-28-during-non-apoptotic-development
#8
Benjamin P Weaver, Yi M Weaver, Shohei Mitani, Min Han
Recent findings suggest that components of the classical cell death machinery also have important non-cell-death (non-apoptotic) functions in flies, nematodes, and mammals. However, the mechanisms for non-canonical caspase substrate recognition and proteolysis, and the direct roles for caspases in gene expression regulation, remain largely unclear. Here we report that CED-3 caspase and the Arg/N-end rule pathway cooperate to inactivate the LIN-28 pluripotency factor in seam cells, a stem-like cell type in Caenorhabditis elegans, thereby ensuring proper temporal cell fate patterning...
June 19, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28601634/synthesis-and-assembly-of-hepatitis-b-virus-envelope-protein-derived-particles-in-escherichia-coli
#9
Hao Li, Keisuke Onbe, Qiushi Liu, Masumi Iijima, Kenji Tatematsu, Masaharu Seno, Hiroko Tada, Shun' Ichi Kuroda
Hepatitis B virus (HBV) envelope particles have been synthesized in eukaryotic cells (e.g., mammalian cells, insect cells, and yeast cells) as an HB vaccine immunogen and drug delivery system (DDS) nanocarrier. Many researchers had made attempts to synthesize the particles in Escherichia coli for minimize the cost and time for producing HBV envelope particles, but the protein was too deleterious to be synthesized in E. coli. In this study, we generated deletion mutants of HBV envelope L protein (389 amino acid residues (aa)) containing three transmembrane domains (TM1, TM2, TM3)...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28581033/life-and-death-of-proteins-after-protease-cleavage-protein-degradation-by-the-n-end-rule-pathway
#10
REVIEW
Nico Dissmeyer, Susana Rivas, Emmanuelle Graciet
The N-end rule relates the stability of a protein to the identity of its N-terminal residue and some of its modifications. Since its discovery in the 1980s, the repertoire of N-terminal degradation signals has expanded, leading to a diversity of N-end rule pathways. Although some of these newly discovered N-end rule pathways remain largely unexplored in plants, recent discoveries have highlighted roles of N-end rule-mediated protein degradation in plant defense against pathogens and in cell proliferation during organ growth...
June 5, 2017: New Phytologist
https://www.readbyqxmd.com/read/28578682/secondary-distress-in-violence-researchers-a-randomised-trial-of-the-effectiveness-of-group-debriefings
#11
Heidi Grundlingh, Louise Knight, Dipak Naker, Karen Devries
BACKGROUND: Secondary distress including emotional distress, vicarious trauma (VT) and secondary traumatic stress (STS) due to exposure to primary trauma victims have been described in helping professionals and in violence researchers. To our knowledge, there are few prevalence studies, and no tailored interventions have been tested to reduce secondary distress in violence researchers. The study aims to (1) describe the epidemiology of secondary distress experienced by violence researchers; to (2) assess the effectiveness of group debriefings in mitigating secondary distress; to (3) assess risk and protective factors...
June 2, 2017: BMC Psychiatry
https://www.readbyqxmd.com/read/28545574/initiating-change-locally-in-bullying-and-aggression-through-the-school-environment-inclusive-trial-update-to-cluster-randomised-controlled-trial-protocol
#12
Chris Bonell, Anne Mathiot, Elizabeth Allen, Leonardo Bevilacqua, Deborah Christie, Diana Elbourne, Adam Fletcher, Richard Grieve, Rosa Legood, Stephen Scott, Emily Warren, Meg Wiggins, Russell M Viner
BACKGROUND: Systematic reviews suggest that multi-component interventions are effective in reducing bullying victimisation and perpetration. We are undertaking a phase III randomised trial of the INCLUSIVE multi-component intervention. This trial aims to assess the effectiveness and cost-effectiveness of the INCLUSIVE intervention in reducing aggression and bullying victimisation in English secondary schools. This paper updates the original trial protocol published in 2014 (Trials 15:381, 2014) and presents the changes in the process evaluation protocol and the secondary outcome data collection...
May 25, 2017: Trials
https://www.readbyqxmd.com/read/28534225/biomarker-monitoring-of-controlled-dietary-acrylamide-exposure-indicates-consistent-human-endogenous-background
#13
Katharina Goempel, Laura Tedsen, Meike Ruenz, Tamara Bakuradze, Dorothea Schipp, Jens Galan, Gerhard Eisenbrand, Elke Richling
The aim of the present study was to explore the relation of controlled dietary acrylamide (AA) intake with the excretion of AA-related urinary mercapturic acids (MA), N-acetyl-S-(carbamoylethyl)-L-cysteine (AAMA) and N-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-L-cysteine (GAMA). Excretion kinetics of these short-term exposure biomarkers were monitored under strictly controlled conditions within a duplicate diet human intervention study. One study arm (group A, n = 6) ingested AA via coffee (0.15-0.17 µg/kg bw) on day 6 and in a meal containing an upper exposure level of AA (14...
May 22, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28528096/analysis-of-domestic-refrigerator-temperatures-and-home-storage-time-distributions-for-shelf-life-studies-and-food-safety-risk-assessment
#14
Anna Roccato, Mieke Uyttendaele, Jeanne-Marie Membré
In the framework of food safety, when mimicking the consumer phase, the storage time and temperature used are mainly considered as single point estimates instead of probability distributions. This singlepoint approach does not take into account the variability within a population and could lead to an overestimation of the parameters. Therefore, the aim of this study was to analyse data on domestic refrigerator temperatures and storage times of chilled food in European countries in order to draw general rules which could be used either in shelf-life testing or risk assessment...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28526524/visual-snow-in-a-paediatric-patient
#15
E Santos-Bueso, A M Muñoz-Hernández, N Avalos-Franco, S García-Sáenz, F Sáenz-Francés, J Porta-Etessam
CASE REPORT: The case is presented of an 11 year-old girl referring to a one year history of photophobia and continuously seeing white spots in both eyes. The patient had a visual acuity of unity in both eyes, and a normal eye examination, and was referred to the Neuro-ophthalmology Unit. Once complete laboratory and imaging tests ruled out the possibility of any neurological pathology, she was diagnosed with visual snow (VS). DISCUSSION: VS is an isolated symptom, possibly part of the migraine aura, that is referred by patients reporting numerous and constant white dots moving in the visual field of BE...
May 16, 2017: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/28515311/control-of-hsp90-chaperone-and-its-clients-by-n-terminal-acetylation-and-the-n-end-rule-pathway
#16
Jang-Hyun Oh, Ju-Yeon Hyun, Alexander Varshavsky
We found that the heat shock protein 90 (Hsp90) chaperone system of the yeast Saccharomyces cerevisiae is greatly impaired in naa10Δ cells, which lack the NatA N(α)-terminal acetylase (Nt-acetylase) and therefore cannot N-terminally acetylate a majority of normally N-terminally acetylated proteins, including Hsp90 and most of its cochaperones. Chk1, a mitotic checkpoint kinase and a client of Hsp90, was degraded relatively slowly in wild-type cells but was rapidly destroyed in naa10Δ cells by the Arg/N-end rule pathway, which recognized a C terminus-proximal degron of Chk1...
May 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28510571/second-line-pazopanib-in-patients-with-relapsed-and-refractory-small-cell-lung-cancer-a-multicentre-phase-ii-study-of-the-hellenic-oncology-research-group
#17
F Koinis, S Agelaki, V Karavassilis, N Kentepozidis, E Samantas, S Peroukidis, P Katsaounis, E Hartabilas, I I Varthalitis, I Messaritakis, G Fountzilas, V Georgoulias, A Kotsakis
BACKGROUND: Pazopanib is a tyrosine kinase inhibitor with antiangiogenic activity. Vascular endothelial growth factor expression is increased in SCLC and is correlated with poor prognosis. The efficacy and tolerance of second-line pazopanib in SCLC was evaluated. PATIENTS AND METHODS: Patients with platinum-sensitive (cohort A; n=39) and -resistant/refractory (cohort B; n=19) SCLC were enrolled in a multicentre phase II study. The primary end point was the progression-free survival rate (PFS-R) at week 8 in each cohort...
June 27, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28506137/elevation-of-serum-park7-and-il-8-levels-is-associated-with-acute-lung-injury-in-patients-with-severe-sepsis-septic-shock
#18
Xiao-Wei Liu, Tao Ma, Quan Cai, Li Wang, Hong-Wei Song, Zhi Liu
OBJECTIVE: Methods containing only clinical information fail to meet the needs of prediction of acute lung injury (ALI) because of the relatively low positive predictive value. This study aimed to investigate the feasibility of using biomarkers as predictors of ALI in populations with severe sepsis/septic shock and to explore difference among biomarkers after adjustment for potential confounders. METHODS: Serum specimens were collected from patients with severe sepsis/septic shock (n = 172) presented to the emergency department...
January 1, 2017: Journal of Intensive Care Medicine
https://www.readbyqxmd.com/read/28445535/microrna-125a-3p-is-regulated-by-myd88-in-legionella-pneumophila-infection-and-targets-ntan1
#19
Elisa Jentho, Malena Bodden, Christine Schulz, Anna-Lena Jung, Kerstin Seidel, Bernd Schmeck, Wilhelm Bertrams
BACKGROUND: Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. It is highly adapted to intracellular replication and manipulates host cell functions like vesicle trafficking and mRNA translation to its own advantage. However, it is still unknown to what extent microRNAs (miRNAs) are involved in the Legionella-host cell interaction. METHODS: WT and MyD88-/- murine bone marrow-derived macrophages (BMM) were infected with L. pneumophila, the transcriptome was analyzed by high throughput qPCR array (microRNAs) and conventional qPCR (mRNAs), and mRNA-miRNA interaction was validated by luciferase assays with 3´-UTR mutations and western blot...
2017: PloS One
https://www.readbyqxmd.com/read/28443142/effects-of-androgen-deprivation-therapy-on-hypercoagulability-in-prostate-cancer-patients-a-prospective-longitudinal-study
#20
Harmanpreet Kaur, D Robert Siemens, Angela Black, Sylvia Robb, Spencer Barr, Charles H Graham, Maha Othman
INTRODUCTION: Androgen-deprivation therapy (ADT) is the mainstay of systemic therapy for advanced prostate cancer (PCa), but has significant adverse effects, including increasing concern for cardiovascular (CV) and thromboembolic (TE) complications. This study carefully investigates any relationship between ADT use and hypercoagulability as a possible mechanism of these adverse effects. METHODS: We performed a prospective, longitudinal study in a cohort of patients with advanced PCa initiating ADT (n=18)...
January 2017: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
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