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https://www.readbyqxmd.com/read/27933735/danazol-has-potential-to-cause-pkc-translocation-cell-cycle-dysregulation-and-apoptosis-in-breast-cancer-cells
#1
Suman Jyoti Deka, Ashalata Roy, Vibin Ramakrishnan, Debasis Manna, Vishal Trivedi
Danazol, the established clinical drug has given promising therapeutic results in a series of clinical trials with breast cancer patients. Danazol shares structural similarities with several known PKC agonists and fits well into the C1 domain. Danazol binds to the C1b domain of PKC with Kd of 5.64±1.27 μM. MD simulation studies further support that the PKC-Danazol molecular model is stable and showing minimum distortion to the structure during the simulation period. Immunofluorescence and western-blotting studies indicate that MDAMB-231 cells stimulated with danazol exhibit translocation of PKCα to the plasma membrane...
December 9, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27933295/sperm-chromatin-dispersion-test-before-sperm-preparation-is-predictive-of-clinical-pregnancy-in-cases-of-unexplained-infertility-treated-with-intrauterine-insemination-and-induction-with-clomiphene-citrate
#2
Frank W R C Vandekerckhove, Ilse De Croo, Jan Gerris, Etienne Vanden Abbeel, Petra De Sutter
BACKGROUND/AIMS: A large proportion of men with normal sperm results as analyzed using conventional techniques have fragmented DNA in their spermatozoa. We performed a prospective study to examine the incidence of DNA fragmentation in sperm in cases of couples with previously unexplained infertility and treated with intrauterine insemination. We evaluated whether there was any predictive value of DNA fragmentation for pregnancy outcome in such couples. METHODS: The percentage of DNA fragmentation and all classical variables to evaluate sperm before and after sperm treatment were determined...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27928873/lycopene-inhibits-regulator-of-calcineurin-1-mediated-apoptosis-by-reducing-oxidative-stress-and-down-regulating-nucling-in-neuronal-cells
#3
Seiyoung Lim, Sinwoo Hwang, Ji Hoon Yu, Joo Weon Lim, Hyeyoung Kim
SCOPE: Regulator of calcineurin 1 (RCAN1) is located on the Down syndrome critical region (DSCR) locus in human chromosome 21. Oxidative stress and overexpression of RCAN1 is implicated in neuronal impairment in Down's syndrome (DS) and Alzheimer's disease (AD). Serum level of lycopene, an antioxidant pigment, is low in DS and AD patients, which may be related to neuronal damage. The present study is to investigate whether lycopene inhibits apoptosis by reducing ROS levels, NF-κB activation, expression of the apoptosis regulator Nucling, cell viability, and indices of apoptosis (cytochrome c release, caspase-3 activation) in RCAN1-overexpressing neuronal cells...
December 8, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27928708/withania-somnifera-reverses-transactive-response-dna-binding-protein-43-proteinopathy-in-a-mouse-model-of-amyotrophic-lateral-sclerosis-frontotemporal-lobar-degeneration
#4
Kallol Dutta, Priyanka Patel, Reza Rahimian, Daniel Phaneuf, Jean-Pierre Julien
Abnormal cytoplasmic mislocalization of transactive response DNA binding protein 43 (TARDBP or TDP-43) in degenerating neurons is a hallmark of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). Our previous work suggested that nuclear factor kappa B (NF-κB) may constitute a therapeutic target for TDP-43-mediated disease. Here, we investigated the effects of root extract of Withania somnifera (Ashwagandha), an herbal medicine with anti-inflammatory properties, in transgenic mice expressing a genomic fragment encoding human TDP-43(A315T) mutant...
December 7, 2016: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
https://www.readbyqxmd.com/read/27928486/phylogenetic-background-of-enterotoxigenic-and-enteroinvasive-escherichia-coli-from-patients-with-diarrhea-in-sirjan-iran
#5
Taifeh Hoseinzadeh, Reza Ghanbarpour, Farokh Rokhbakhsh-Zamin
BACKGROUND AND OBJECTIVES: Diarrheagenic Escherichia coli (DEC) strains are a major cause of intestinal syndromes in the developing countries. The aim of this study was to determine the prevalence of enterotoxigenic E. coli (ETEC) and enteroinvasive E. coli (EIEC) in relation to phylogenetic background from patients with diarrhea. MATERIALS AND METHODS: A total of 110 E. coli isolates were obtained from diarrhea patients in Sirjan, southeast of Iran. The E. coli isolates were confirmed using biochemical and bacteriological tests...
June 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/27928484/the-clonal-relationship-among-the-citrobacter-freundii-isolated-from-the-main-hospital-in-kermanshah-west-of-iran
#6
Mansour Rezaei, Alisha Akya, Azam Elahi, Keyghobad Ghadiri, Somayeh Jafari
BACKGROUND AND OBJECTIVES: Citrobacter freundii is an opportunistic pathogen causing nosocomial infections and resistant to various antibiotics. We aimed to determine the clonal relationship of C. freundii isolates using Pulsed-Field Gel Electrophoresis (PFGE). MATERIALS AND METHODS: Fifty clinical isolates of C. freundii were collected from the main hospital in Kermanshah. After antibiotic susceptibility testing and screening for extended-spectrum beta lactamase (ESBL), all isolates were genotyped by PFGE...
June 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/27926480/calcium-dependent-binding-of-myc-to-calmodulin
#7
Philipp Raffeiner, Andrea Schraffl, Thomas Schwarz, Ruth Röck, Karin Ledolter, Markus Hartl, Robert Konrat, Eduard Stefan, Klaus Bister
The bHLH-LZ (basic region/helix-loop-helix/leucine zipper) oncoprotein Myc and the bHLH-LZ protein Max form a binary transcription factor complex controlling fundamental cellular processes. Deregulated Myc expression leads to neoplastic transformation and is a hallmark of most human cancers. The dynamics of Myc transcription factor activity are post-translationally coordinated by defined protein-protein interactions. Here, we present evidence for a second messenger controlled physical interaction between the Ca2+ sensor calmodulin (CaM) and all Myc variants (v-Myc, c-Myc, N-Myc, and L-Myc)...
December 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27925065/natural-canine-infection-by-leishmania-infantum-and-leishmania-amazonensis-and-their-implications-for-disease-control
#8
Letícia da Cruz Sanches, Cleber Costa de Martini, Alex Akira Nakamura, Maria Emília Bodini Santiago, Beatriz Dolabela de Lima, Valéria Marçal Felix de Lima
Leishmaniasis is a major public health problem worldwide. Because Leishmania can adapt to new hosts or vectors, knowledge concerning the current etiological agent in dogs is important in endemic areas. This study aimed to identify the Leishmania species detected in 103 samples of peripheral blood from dogs that were naturally infected with these protozoa. The diagnosis of leishmaniasis was determined through parasitological examination, the indirect enzyme-linked immunosorbent assay (ELISA) and the polymerase chain reaction (PCR)...
December 1, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27925060/hepatozoon-canis-and-leishmania-spp-coinfection-in-dogs-diagnosed-with-visceral-leishmaniasis
#9
Fernanda Nazaré Morgado, Amanda Dos Santos Cavalcanti, Luisa Helena de Miranda, Lúcia Helena O'Dwyer, Maria Regina Lucas da Silva, Rodrigo Caldas Menezes, Aurea Virgínia Andrade da Silva, Mariana Côrtes Boité, Elisa Cupolillo, Renato Porrozzi
This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1' PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR...
December 1, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27923691/growth-inhibitory-effect-of-scrophularia-oxysepala-extract-on-mouse-mammary-carcinoma-4t1-cells-in-vitro-and-in-vivo-systems
#10
Pooneh Chokhachi Baradaran, Ali Mohammadi, Behzad Mansoori, Sepideh Chokhachi Baradaran, Behzad Baradaran
INTRODUCTION: Medical plants have been intensively studied as a source of antitumor compounds. In the present study, we determine the effect of Scrophularia oxysepala on triggering apoptosis and diminishing growth, size and weight of the tumor in the allograft model of Balb/c mice. MATERIAL & METHODS: The cytotoxic effects of Scrophularia oxysepala extract on 4T1 cells were studied using MTT (3-[4,5-dimethyl-2-thiazolyl]-2, 5 diphenyl tetrazolium bromide) assay and Trypan blue staining...
December 3, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27923682/chitosan-nanoparticles-plus-quercetin-suppress-the-oxidative-stress-modulate-dna-fragmentation-and-gene-expression-in-the-kidney-of-rats-fed-ochratoxin-a-contaminated-diet
#11
Mosaad A Abdel-Wahhab, Abdulhadi Aljawish, Aziza A El-Nekeety, Sekena H Abdel-Aiezm, Nabila S Hassan
This study aimed to evaluate the protective role of chitosan nanoparticles (COS-NPs) singly or plus quercetin (Q) against OTA-induced oxidative stress and renal genotoxicity. Twelve groups of male Sprague-Dawley rats were treated orally for 3 weeks included the control group, animals fed OTA-contaminated diet (3 mg/kg diet); COS-NPs-treated groups at low (140 mg/kg b.w.) or high (280 mg/kg b.w.) dose, Q-treated group (50 mg/kg b.w.), Q plus low or high dose of COS-NPs-treated groups and OTA plus Q and/or COS-NPs at the two tested doses-treated groups...
December 3, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27923681/evaluation-of-dna-damaging-potential-of-bisphenol-a-and-its-selected-analogs-in-human-peripheral-blood-mononuclear-cells-in-vitro-study
#12
Katarzyna Mokra, Agnieszka Kuźmińska-Surowaniec, Katarzyna Woźniak, Jaromir Michałowicz
In the present study, we have investigated DNA-damaging potential of BPA and its analogs, i.e. bisphenol S (BPS), bisphenol F (BPF) and bisphenol AF (BPAF) in human peripheral blood mononuclear cells (PBMCs) using the alkaline and neutral versions of the comet assay, which allowed to evaluate DNA single strand-breaks (SSBs) and double strandbreaks (DSBs). The use of the alkaline version of comet assay made also possible to analyze the kinetics of DNA repair in PBMCs after exposure of the cells to BPA or its analogs...
December 3, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27923366/3c-digital-pcr-for-quantification-of-chromatin-interactions
#13
Meijun Du, Liang Wang
BACKGROUND: Chromosome conformation capture (3C) is a powerful and widely used technique for detecting the physical interactions between chromatin regions in vivo. The principle of 3C is to convert physical chromatin interactions into specific DNA ligation products, which are then detected by quantitative polymerase chain reaction (qPCR). However, 3C-qPCR assays are often complicated by the necessity of normalization controls to correct for amplification biases. In addition, qPCR is often limited to a certain cycle number, making it difficult to detect fragment ligations with low frequency...
December 6, 2016: BMC Molecular Biology
https://www.readbyqxmd.com/read/27921370/not-just-for-coding-a-new-role-for-histone-tails-in-replication-enzyme-activation
#14
Anja-Katrin Bielinsky, Wendy Leung
The processing of Okazaki fragments when they are assembled into nucleosomes has received little attention. In this issue of The FEBS Journal, Seo and colleagues show that binding to histone tails stimulates the enzymatic activity of flap endonuclease 1 (Rad27). Histone tails are structurally similar to the C terminus of Rad27 and can thus mimic its autostimulatory function. This study highlights an active regulatory role for nucleosomes on DNA metabolism.
December 2016: FEBS Journal
https://www.readbyqxmd.com/read/27920888/development-of-a-high-resolution-melting-analysis-method-for-cyp2c19-17-genotyping-in-healthy-volunteers
#15
Zahra Ghasemi, Mehrdad Hashemi, Mahsa Ejabati, Seyyed Meisam Ebrahimi, Hamidreza Kheiri Manjili, Ali Sharafi, Ali Ramazani
BACKGROUND: Genetic polymorphisms of drug metabolisms by cytochrome P450 (P450s) could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting (HRM) method compared to PCR-RFLP for genotyping healthy Iranian population. METHODS: Blood samples were collected from 100 healthy Iranian volunteers...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920275/hypermethylation-of-brain-natriuretic-peptide-bnp-gene-is-associated-with-the-risk-of-rheumatic-heart-disease
#16
Ni Li, Dawei Zheng, Lebo Sun, Huoshun Shi, Xiuying Zhu, Guodong Xu, Qinning Wang, Caimin Zhu, Guofeng Shao
To investigate the contribution of BNP promoter DNA methylation to the risk of rheumatic heart disease (RHD) and the influence of warfarin anticoagulant therapy on BNP methylation levels for RHD patients after surgery.BNP methylation levels were determined by bisulfite pyrosequencing from plasma samples of RHD patients compared with healthy controls. Several factors influencing the RHD patients were included age, smoking, and cholesterol levels. A fragment of 5 CG sites (CpG1-5) in the promoter region of BNP gene was measured...
December 5, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27919644/comparative-investigation-of-methionine-and-novel-formulation-metovitan-protective-effects-in-wistar-rats-with-testicular-and-epididymal-toxicity-induced-by-anti-tuberculosis-drugs-co-administration
#17
Ganna M Shayakhmetova, Larysa B Bondarenko, Alla K Voronina, Valentina M Kovalenko
Despite clear beneficial effects, studies on the efficacy of different formulations of micronutrients and vitamins in the treatment of male infertility are still very limited. The aim of the study was to compare the efficacy of methionine and novel formulation Metovitan based on the combination of methionine, thiamine, nicotinamide, α-tocopherol acetate and zinc salt as remedies for prevention of anti-tuberculosis drugs (ATD) anti-fertility effects in male rats. ATD co-administration resulted in testicular CYP2E1, CYP2C23 and CYP3A2 transcriptional activation...
December 2, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27919246/adjacent-sequences-disclose-potential-for-intra-genomic-dispersal-of-satellite-dna-repeats-and-suggest-a-complex-network-with-transposable-elements
#18
Eva Satović, Tanja Vojvoda Zeljko, Andrea Luchetti, Barbara Mantovani, Miroslav Plohl
BACKGROUND: Satellite DNA (satDNA) sequences are typically arranged as arrays of tandemly repeated monomers. Due to the similarity among monomers, their organizational pattern and abundance, satDNAs are hardly accessible to structural and functional studies and still represent the most obscure genome component. Although many satDNA arrays of diverse length and even single monomers exist in the genome, surprisingly little is known about transition from satDNAs to other sequences. Studying satDNA monomers at junctions and identifying DNA sequences adjacent to them can help to understand the processes that (re)distribute satDNAs and significance that evolution of these sequence elements might have in creating the genomic landscape...
December 6, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27917170/isolation-and-characterization-of-acinetobacter-baumannii-recovered-from-campylobacter-selective-medium
#19
Dinesh M Fernando, Izhar U H Khan, Rakesh Patidar, David R Lapen, Guylaine Talbot, Edward Topp, Ayush Kumar
Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates of A. baumannii growing under conditions selective for Campylobacter spp. (microaerophilic at 42°C and in the presence of antibiotics) from dairy cattle manure storage tank or surface water impacted by livestock effluents...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27916276/a-surveillance-mechanism-ensures-repair-of-dna-lesions-during-zygotic-reprogramming
#20
Sabrina Ladstätter, Kikuë Tachibana-Konwalski
Sexual reproduction culminates in a totipotent zygote with the potential to produce a whole organism. Sperm chromatin reorganization and epigenetic reprogramming that alter DNA and histone modifications generate a totipotent embryo. Active DNA demethylation of the paternal genome has been proposed to involve base excision and DNA repair-based mechanisms. The nature and consequence of DNA lesions generated during reprogramming are not known. Using mouse genetics and chemical biology, we discovered that Tet3-dependent zygotic reprogramming generates paternal DNA lesions that are monitored by a surveillance mechanism...
November 21, 2016: Cell
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