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Phosphorylated insulin like growth factor

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https://www.readbyqxmd.com/read/29138637/regulation-of-osteogenic-differentiation-of-placental-derived-mesenchymal-stem-cells-by-insulin-like-growth-factors-and-low-oxygen-tension
#1
Amer Youssef, Victor K M Han
Placental mesenchymal stem cells (PMSCs) are multipotent cells that can differentiate in vitro to multiple lineages, including bone. Insulin-like growth factors (IGFs, IGF-1 and IGF-2) participate in maintaining growth, survival, and differentiation of many stem cells, including osteoprogenitors. Low oxygen tension (PO2) can maintain stem cell multipotency and impede osteogenic differentiation. In this study, we investigated whether PMSC osteogenic differentiation is influenced by low PO2 and by IGFs. Our results indicated that low PO2 decreased osteogenic markers RUNX2 and OPN; however, re-exposure to higher oxygen tension (room air) restored differentiation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29137928/camkii%C3%AE-expression-in-a-mouse-model-of-nmdar-hypofunction-schizophrenia-putative-roles-for-igf-1r-and-tlr4
#2
O M Ogundele, C C Lee
Schizophrenia (SCZ) is a neuropsychiatric disorder that is linked to social behavioral deficits and other negative symptoms associated with hippocampal synaptic dysfunction. Synaptic mechanism of schizophrenia is characterized by loss of hippocampal N-Methyl-d-Aspartate Receptor (NMDAR) activity (NMDAR hypofunction) and dendritic spines. Previous studies show that genetic deletion of hippocampal synaptic regulatory calcium-calmodulin dependent kinase II alpha (CaMKIIα) cause synaptic and behavioral defects associated with schizophrenia in mice...
November 11, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/29118927/topical-insulin-accelerates-cutaneous-wound-healing-in-insulin-resistant-diabetic-rats
#3
Tianyi Yu, Min Gao, Peilang Yang, Qing Pei, Dan Liu, Di Wang, Xiong Zhang, Yan Liu
Insulin signaling defects could lead to insulin resistance in insulin target organs: typically, in the muscler, liver, and adipose tissue. We have observed that insulin accelerated diabetic wound healing in our previous works; to further elucidate the mechanism, we investigated the expression and activation of insulin and insulin-like growth factor (IGF)-1 signaling, compared insulin sensitivity in skin tissue with that in liver tissue, and also observed the regulation of insulin on inflammatory response of wounds during the healing process...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29110181/c-myb-plays-an-essential-role-in-the-protective-function-of-igf-1-on-cytotoxicity-induced-by-a%C3%AE-25-35-via-the-pi3k-akt-pathway
#4
Jingyu Zhang, Yongwei Shu, Yang Qu, Lina Zhang, Tingting Chu, Yonghui Zheng, Hong Zhao
There have been numerous reports about neurodegenerative diseases, including Alzheimer's disease. Nevertheless, the molecules responsible for the neurodegeneration in Alzheimer's disease are basically unknown. Recent findings indicate that the cellular myeloblastosis (c-myb) regulates neural progenitor cell proliferation. In the current study, the function of insulin-like growth factor-1 (IGF-1) against cell toxicity in SH-SY5Y cells induced by β-amyloid 25-35 (Aβ25-35) and its molecular mechanism were investigated...
November 6, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29104486/tanshinone-iia-inhibits-%C3%AE-catenin-nuclear-translocation-and-igf-2r-activation-via-estrogen-receptors-to-suppress-angiotensin-ii-induced-h9c2-cardiomyoblast-cell-apoptosis
#5
Ya-Fang Chen, Cecilia Hsuan Day, Nien-Hung Lee, Yu-Feng Chen, Jaw-Ji Yang, Chih-Hsueh Lin, Ray-Jade Chen, Peramaiyan Rajendran, Vijaya Padma Viswanadha, Chih-Yang Huang
Cardiomyopathy involves changes in the myocardial ultra-structure, hypertrophy, apoptosis, fibrosis and inflammation. Angiotensin II (AngII) stimulates the expression of insulin like-growth factors (IGF-2) and IGF-2 receptor (IGF-2R) in H9c2 cardiomyoblasts and subsequently leads to apoptosis. Estrogen receptors protect cardiomyocytes from apoptosis and fibrosis. Tanshinone IIA (TSN), a main active ingredient from Danshen, has been shown to protect cardiomyocytes from death caused by different stress signals...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29100507/inhibition-of-pi3k-akt-mtor-overcomes-cisplatin-resistance-in-the-triple-negative-breast-cancer-cell-line-hcc38
#6
Katharina Gohr, Alexandra Hamacher, Laura H Engelke, Matthias U Kassack
BACKGROUND: Widely established targeted therapies directed at triple negative breast cancer (TNBC) are missing. Classical chemotherapy remains the systemic treatment option. Cisplatin has been tested in TNBC but bears the disadvantage of resistance development. The purpose of this study was to identify resistance mechanisms in cisplatin-resistant TNBC cell lines and select targeted therapies based on these findings. METHODS: The TNBC cell lines HCC38 and MDA-MB231 were subjected to intermittent cisplatin treatment resulting in the 3...
November 3, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29099264/co-targeting-pi3k-mtor-and-igf1r-with-small-molecule-inhibitors-for-treating-undifferentiated-pleomorphic-sarcoma
#7
Caitlin D May, Sharon M Landers, Svetlana Bolshakov, XiaoYan Ma, Davis R Ingram, Christine M Kivlin, Kelsey L Watson, Ghadah A Al Sannaa, Angela D Bhalla, Wei-Lien Wang, Alexander J Lazar, Keila E Torres
Undifferentiated pleomorphic sarcomas (UPSs) are aggressive mesenchymal malignancies with no definitive cell of origin or specific recurrent genetic hallmarks. These tumors are largely chemoresistant; thus, identification of potential therapeutic targets is necessary to improve patient outcome. Previous studies demonstrated that high expression of activated protein kinase B (AKT) in patients with UPS corresponds to poor disease-specific survival. Here, we demonstrate that inhibiting phosphatidylinositol-3-kinase/mammalian target of rapamycin (PI3K/mTOR) signaling using a small molecule inhibitor reduced UPS cell proliferation and motility and xenograft growth; however, increased phosphorylation of insulin-like growth factor 1 receptor (IGF1R) indicated the potential for adaptive resistance following treatment through compensatory receptor activation...
October 3, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29080043/pi3k-akt-mtor-pathway-involvement-in-regulating-growth-hormone-secretion-in-a-rat-pituitary-adenoma-cell-line
#8
Carmelina Di Pasquale, Erica Gentilin, Simona Falletta, Mariaenrica Bellio, Mattia Buratto, Ettore Degli Uberti, Maria Chiara Zatelli
PURPOSE: Insulin-like growth factor 1 (IGF1) controls growth hormone (GH) secretion via a negative feed-back loop that may disclose novel mechanisms possibly useful to control GH hyper-secretion. Our aim was to understand whether PI3K/Akt/mTOR pathway is involved in IGF1 negative feedback on GH secretion. METHODS: Cell viability, GH secretion, Akt, and Erk 1/2 phosphorylation levels in the rat GH3 cell line were assessed under treatment with IGF1 and/or everolimus, an mTOR inhitior...
October 27, 2017: Endocrine
https://www.readbyqxmd.com/read/29079753/norrin-protects-optic-nerve-axons-from-degeneration-in-a-mouse-model-of-glaucoma
#9
Stephanie A Leopold, Ludwig F Zeilbeck, Gregor Weber, Roswitha Seitz, Michael R Bösl, Herbert Jägle, Rudolf Fuchshofer, Ernst R Tamm, Andreas Ohlmann
Norrin is a secreted signaling molecule activating the Wnt/β-catenin pathway. Since Norrin protects retinal neurons from experimental acute injury, we were interested to learn if Norrin attenuates chronic damage of retinal ganglion cells (RGC) and their axons in a mouse model of glaucoma. Transgenic mice overexpressing Norrin in the retina (Pax6-Norrin) were generated and crossed with DBA/2J mice with hereditary glaucoma and optic nerve axonal degeneration. One-year old DBA/2J/Pax6-Norrin animals had significantly more surviving optic nerve axons than their DBA/2J littermates...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29073599/combination-of-solamargine-and-metformin-strengthens-igfbp1-gene-expression-through-inactivation-of-stat3-and-reciprocal-interaction-between-foxo3a-and-sp1
#10
Qing Tang, Fang Zheng, JingJing Wu, Qian Xiao, Liuning Li, Swei Sunny Hann
BACKGROUND/AIMS: Solamargine, one natural photochemical component from traditional plants, has been shown to have anti-cancers properties. We previously showed that solamargine inhibited the growth of non-small-cell lung cancer (NSCLC) cells through suppression of prostaglandin E2 (PGE2) receptor EP4 gene and regulation of downstream signaling pathways. However, the detailed mechanism underlying this, especially in combination of metformin, a known AMPK activator, still remained to be determined...
October 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29072778/regulation-of-kisspeptin-synthesis-and-release-in-the-preoptic-anterior-hypothalamic-region-of-prepubertal-female-rats-actions-of-igf-1-and-alcohol
#11
Jill K Hiney, Vinod K Srivastava, Danielle N Vaden Anderson, Nicole L Hartzoge, William L Dees
Alcohol (ALC) causes suppressed secretion of prepubertal luteinizing hormone-releasing hormone (LHRH). Insulin-like growth factor-1 (IGF-1) and kisspeptin (Kp) are major regulators of LHRH and are critical for puberty. IGF-1 may be an upstream mediator of Kp in the preoptic area and rostral hypothalamic area (POA/RHA) of the rat brain, a region containing both Kp and LHRH neurons. We investigated the ability of IGF-1 to stimulate prepubertal Kp synthesis and release in POA/RHA, and the potential inhibitory effects of ALC METHODS: Immature female rats were administered either ALC (3g/Kg) or water via gastric gavage at 0730 h...
October 26, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29057462/igf-1-activates-pi3k-akt-signaling-to-protect-human-retinal-pigment-epithelial-cells-from-amiodarone-induced-oxidative-injury
#12
Rifang Liao, Fengxia Yan, Zhuanping Zeng, Haitao Wang, Kaifeng Qiu, Jinying Xu, Wenhua Zheng
BACKGROUND AND PURPOSE: Amiodarone (AM) is one of the most effective anti-arrhythmic drugs available. However, clinical applications of AM are limited by its toxic side effects including optic toxicity. The purpose of this study was to investigate the toxic effect of AM on D407 cells (a human Retinal Pigmented Epithelial cell line) and the mechanisms of the protective effect of insulin-like growth factor-1 (IGF-1). EXPERIMENTAL APPROACH: The involvement of the kinases, Akt and Erk, was analyzed by Western blot...
October 22, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29051069/procyanidins-from-cinnamomi-cortex-promote-proteasome-independent-degradation-of-nuclear-nrf2-through-phosphorylation-of-insulin-like-growth-factor-1-receptor-in-a549%C3%A2-cells
#13
Tomokazu Ohnuma, Kazuya Sakamoto, Asumi Shinoda, Chiaki Takagi, Shoko Ohno, Takahito Nishiyama, Kenichiro Ogura, Akira Hiratsuka
Many lines of evidence demonstrate that transcription factor nuclear factor-E2-related factor 2 (Nrf2) plays essential roles in cancer cell proliferation and resistance to chemotherapy, thereby indicating that suppression of abnormal Nrf2 activation is needed for a new therapeutic approach. Our previous studies reported that procyanidins prepared from Cinnamomi Cortex extract (CCE) have an ability to suppress cytoprotective enzymes and cell proliferation in human cancer cells with activated Nrf2. In the present study, we investigated the mechanism of CCE procyanidin-mediated antagonization of Nrf2...
December 1, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29042931/delivery-of-biotinylated-igf-1-with-biotinylated-self-assembling-peptides-combined-with-bone-marrow-stem-cell-transplantation-promotes-cell-therapy-for-myocardial-infarction
#14
Ming Zhang, Wen-Wei Ai, Zhi-Liang Mei, Yun-Hua Hu, Zhi-Ling Zhang
Cell therapy is a promising approach for cardiac repair. The aim of the present study was to determine the feasibility of using biotinylated insulin-like growth factor 1 (IGF-1) with biotinylated self-assembling peptides (tethered IGF-1) combined with bone marrow stem cells (BMSCs) transplantation for the treatment of heart failure. Tethered IGF-1 was synthesized and its effect on H9c2 cells was analyzed. Reverse transcription-quantitative polymerase chain reaction and western blot assays demonstrated that tethered IGF-1 did not significantly affect the expression and phosphorylation of AKT, whereas it significantly increased the expression of cardiac troponin T (P<0...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29039467/insulin-like-growth-factor-binding-protein-6-released-from-human-mesenchymal-stem-cells-confers-neuronal-protection-through-igf-1r-mediated-signaling
#15
Hyo-Jin Jeon, Jihye Park, Joo-Hyun Shin, Mi-Sook Chang
Human bone marrow‑derived mesenchymal stem cells (hMSCs) are a desirable cell source for cell‑based therapy to treat nervous system injuries due to their ability to differentiate into specific cell types. In addition to their multipotency, hMSCs render the tissue microenvironment more favorable for tissue repair by secreting various growth factors. Our previous study demonstrated that hMSCs secrete several growth factors, including several insulin‑like growth factor binding proteins (IGFBPs). Among these, IGFBP‑6 binds with high affinity and inhibits insulin growth factor‑2 (IGF‑2) to inhibit the growth of IGF‑2‑dependent tumors...
October 5, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29037858/co-localization-of-insulin-like-growth-factor-binding-protein-1-casein-kinase-2%C3%AE-and-mechanistic-target-of-rapamycin-in-human-hepatocellular-carcinoma-cells-as-demonstrated-by-dual-immunofluorescence-and-in-situ-proximity-ligation-assay
#16
Sahil S Singal, Karen Nygard, Manthan R Dhruv, Kyle Biggar, Majida Abu Shehab, Shawn Shun-Cheng Li, Thomas Jansson, Madhulika B Gupta
Insulin-like growth factor binding protein (IGFBP)-1 influences fetal growth by modifying insulin-like growth factor-1 bioavailability. IGFBP-1 phosphorylation is regulated by mechanistic target of rapamycin (mTOR) and casein kinase (CSNK)-2. However, the underlying molecular mechanisms remain unknown. We examined the cellular localization and potential interactions of IGFBP-1, CSNK-2β, and mTOR as a prerequisite for protein-protein interaction. Analysis of dual immunofluorescence images indicated a potential perinuclear co-localization between IGFBP-1 and CSNK-2β and a nuclear co-localization between CSNK-2β and mTOR...
October 14, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29029433/h2o2-attenuates-igf-1r-tyrosine-phosphorylation-and-its-survival-signaling-properties-in-neuronal-cells-via-nr2b-containing-nmda-receptor
#17
Zhiwen Zeng, Dejun Wang, Uma Gaur, Liao Rifang, Haitao Wang, Wenhua Zheng
Impairment of insulin-like growth factor I (IGF-I) signaling plays an important role in the development of neurodegeneration. In the present study, we investigated the effect of H2O2 on the survival signaling of IGF-1 and its underlying mechanisms in human neuronal cells SH-SY5Y. Our results showed that IGF-1 promoted cell survival and stimulated phosphorylation of IGF-1R as well as its downstream targets like AKT and ERK1/2 in these cells. Meanwhile, these effects of IGF-1 were abolished by H2O2 at 200μM concentration which did not cause any significant toxicity to cells itself in our experiments...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28987868/impairment-of-akt-activity-by-cyp2e1-mediated-oxidative-stress-is-involved-in-chronic-ethanol-induced-fatty-liver
#18
Tao Zeng, Cui-Li Zhang, Ning Zhao, Min-Jie Guan, Mo Xiao, Rui Yang, Xiu-Lan Zhao, Li-Hua Yu, Zhen-Ping Zhu, Ke-Qin Xie
Protein kinase B (PKB/Akt) plays important roles in the regulation of lipid homeostasis, and impairment of Akt activity has been demonstrated to be involved in the development of non-alcoholic fatty liver disease (NAFLD). Previous studies suggest that cytochrome P4502E1 (CYP2E1) plays causal roles in the pathogenesis of alcoholic fatty liver (AFL). We hypothesized that Akt activity might be impaired due to CYP2E1-induced oxidative stress in chronic ethanol-induced hepatic steatosis. In this study, we found that chronic ethanol-induced hepatic steatosis was accompanied with reduced phosphorylation of Akt at Thr308 in mice liver...
September 27, 2017: Redox Biology
https://www.readbyqxmd.com/read/28952285/hepatic-insulin-like-growth-factor-receptor-is-upregulated-by-activation-of-the-gsk3b-foxo3-pathway-after-partial-hepatectomy
#19
C Y E, Y Cai, B Z Sun, L Y Guan, T Jiang
Liver regeneration after partial hepatectomy (PH) is a complex and well-elaborated biological process whereby synchronized cell proliferation is induced in response to the loss of liver mass. Insulin-like growth factor 1 (IGF1) signaling, which plays a crucial role in normal growth and development, is involved in the process of liver regeneration. To assess the changes in the levels of serum IGF1 and hepatic IGF1 receptor (IGF1R), we established a mouse model for PH. This also allowed us to further explore the mechanisms that participate in the regulation of liver regeneration...
July 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28951744/igf1-and-nrg1-enhance-proliferation-metabolic-maturity-and-the-force-frequency-response-in-hesc-derived-engineered-cardiac-tissues
#20
Cassady E Rupert, Kareen L K Coulombe
Insulin-like growth factor 1 (IGF1) and neuregulin-1β (NRG1) play important roles during cardiac development both individually and synergistically. In this study, we analyze how 3D cardiac tissue engineered from human embryonic stem cell- (hESC-) derived cardiomyocytes and 2D-plated hESC-cardiomyocytes respond to developmentally relevant growth factors both to stimulate maturity and to characterize the therapeutic potential of IGF1 and NRG1. When administered to engineered cardiac tissues, a significant decrease in active force production of ~65% was measured in all treatment groups, likely due to changes in cellular physiology...
2017: Stem Cells International
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