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mycobacterium abscessus

Cecilia Brambilla, Marta Llorens-Fons, Esther Julián, Estela Noguera-Ortega, Cristina Tomàs-Martínez, Miriam Pérez-Trujillo, Thomas F Byrd, Fernando Alcaide, Marina Luquin
The rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. This idea is consistent with the fact that Mycobacterium tuberculosis presents a stable rough morphotype. Unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). Currently, the initial interaction of macrophages with clumps remains unclear. Thus, we infected J774 macrophages with bacterial suspensions of rough morphotypes of M...
2016: Frontiers in Microbiology
Kenneth N Olivier, David E Griffith, Gina Eagle, John P McGinnis Ii, Liza Micioni, Keith Liu, Charles L Daley, Kevin L Winthrop, Stephen Ruoss, Doreen J Addrizzo-Harris, Patrick A Flume, Daniel Dorgan, Matthias Salathe, Barbara A Brown-Elliott, Renu Gupta, Richard J Wallace
Rationale Lengthy multi-drug, toxic, and low efficacy regimens limit management of pulmonary nontuberculous mycobacterial (PNTM) disease. Objective This phase 2 study investigated efficacy and safety of liposomal amikacin for inhalation (LAI) in treatment-refractory PNTM (Mycobacterium avium complex [MAC] or Mycobacterium abscessus) disease. Methods During the double-blind phase, patients were randomly assigned to LAI (590 mg) or placebo once daily added to their multi-drug regimen for 84 days. Both groups could receive open-label LAI for 84 additional days...
October 17, 2016: American Journal of Respiratory and Critical Care Medicine
Eva Polverino, Graham H Bothamley, Delia Goletti, Jan Heyckendorf, Giovanni Sotgiu, Stefano Aliberti
The breadth and quality of scientific presentations on clinical and translational research into respiratory infections at the 2015 European Respiratory Society (ERS) International Congress in Amsterdam, the Netherlands, establishes this area as one of the leadings fields in pulmonology. The host-pathogen relationship in chronic obstructive pulmonary disease, and the impact of comorbidities and chronic treatment on clinical outcomes in patients with pneumonia were studied. Various communications were dedicated to bronchiectasis and, in particular, to different prognostic and clinical aspects of this disease, including chronic infection with Pseudomonas and inhaled antibiotic therapy...
July 2016: ERJ Open Research
Faiza Mougari, Lorenzo Guglielmetti, Laurent Raskine, Isabelle Sermet-Gaudelus, Nicolas Veziris, Emmanuelle Cambau
Mycobacterium abscessus is an emerging mycobacteria that is responsible for lung diseases and healthcare-associated extrapulmonary infections. Recent findings support its taxonomic status as a single species comprising 3 subspecies designated abscessus, bolletii and massiliense. We performed a review of English-language publications investigating all three of these subspecies. Areas covered: Worldwide, human infections are often attributable to environmental contamination, although the isolation of M. abscessus in this reservoir is very rare...
October 3, 2016: Expert Review of Anti-infective Therapy
Liulin Luo, Weijia Liu, Bing Li, Mengling Li, Dongdong Huang, Linjie Jing, Huiping Chen, Jinghui Yang, Jun Yue, Fang Wang, Haiqing Chu, Zhemin Zhang
This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole genome sequencing analysis: 139 M. abscessus subsp. abscessus and 36 M. abscessus subsp. massiliense The research-use-only (RUO) Saramis Knowledge Base database V.4.12 was modified accordingly by adding 40 M...
September 28, 2016: Journal of Clinical Microbiology
Oren Mayer, Paras Jain, Torin Weisbrod, Daniel Biro, Libby Ho, Deborah Jacobs-Sera, Graham F Hatfull, William R Jacobs
: Mycobacteriophage DS6A is unique amongst the more than 8000 isolated mycobacteriophages due to its ability to form plaques exclusively on mycobacteria belonging to the Mycobacterium tuberculosis Complex (MTBC). Speculation surrounding this specificity has led to unsupported assertions in published studies and patents that non-tuberculous mycobacteria (NTM) are wholly resistant to DS6A infection. In this study, we identified two independent, nonessential regions in the DS6A genome and replaced them with an mVenus-expressing plasmid to generate fluorescent reporter phages Φ(2)GFP12 and Φ(2)GFP13...
September 26, 2016: Journal of Bacteriology
K Eustace, V Jolliffe, A Sahota, K Gholam
A 28-year-old man presented with a 10-day history of scalp nodules. He had undergone hair transplantation 2 months previously. Incision and drainage of one of the nodules yielded gelatinous material, which was sent for microscopy and culture, including low-temperature culture. After 2 weeks of incubation, culture of the nodule yielded acid- and alcohol-fast bacilli, which were identified as Mycobacterium abscessus, a rapidly growing, nontuberculous mycobacterium, which has been reported to cause cutaneous, soft tissue and respiratory infections following trauma, surgery or injection with nonsterile needles...
October 2016: Clinical and Experimental Dermatology
Lindsay J Caverly, Theodore Spilker, John J LiPuma
We report the complete genome sequences of 17 rapidly growing nontuberculous mycobacterial (NTM) strains, including 16 Mycobacterium abscessus complex strains and one M. immunogenum strain. These sequences add value to studies of the genetic diversity of rapidly growing NTM strains recovered from human specimens.
2016: Genome Announcements
I Porvaznik, I Solovič, J Mokrý
Non-tuberculous mycobacteria (NTM) are species other than those belonging to the Mycobacterium tuberculosis complex and do not cause leprosy. NTM are generally free-living organisms that are ubiquitous in the environment. There have been more than 140 NTM species identified to-date. They can cause a wide range of infections, with pulmonary infections being the most frequent (65-90 %). There is growing evidence that the incidence of NTM lung diseases and associated hospitalizations are on the rise, mainly in regions with a low prevalence of tuberculosis...
September 20, 2016: Advances in Experimental Medicine and Biology
Yi Sak Kim, Chul-Su Yang, Loi T Nguyen, Jin Kyung Kim, Hyo Sun Jin, Jin Ho Choe, Soo Yeon Kim, Hye-Mi Lee, Mingyu Jung, Jin-Man Kim, Myung Hee Kim, Eun-Kyeong Jo, Ji-Chan Jang
Mycobacterial ESX systems are often related to pathogenesis during infection. However, little is known about the function of ESX systems of Mycobacterium abscessus (Mab). This study focuses on the Mab ESX-3 cluster, which contains major genes such as esxH (Rv0288, low molecular weight protein antigen 7; CFP-7) and esxG (Rv0287, ESAT-6 like protein). An esx-3 (MAB 2224c-2234c)-deletional mutant of Mab (Δesx) was constructed and used to infect murine and human macrophages. We then investigated whether Mab Δesx modulated innate host immune responses in macrophages...
September 13, 2016: Microbes and Infection
Madhumita Talukdar, Dhrubajyoti Das, Chiranjeeta Bora, Tarun Chandra Bora, Hari Prasanna Deka Boruah, Anil Kumar Singh
Micromonospora genus produces >700 bioactive compounds of medical relevance. In spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing Micromonospora strains. Thus, here we contribute the full genome sequence of Micromonospora sp. HK10 strain, which has high antibacterial activity against several important human pathogens like, Mycobacterium abscessus, Mycobacterium smegmatis, Bacillus subtillis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella and Escherichia coli...
December 5, 2016: Gene
Vanessa Albertina Agertt, Pauline Cordenonsi Bonez, Grazielle Guidolin Rossi, Vanessa da Costa Flores, Fallon Dos Santos Siqueira, Caren Rigon Mizdal, Lenice Lorenço Marques, Gelson Noe Manzoni de Oliveira, Marli Matiko Anraku de Campos
Mycobacteriosis is a type of infection caused by rapidly growing mycobacteria (RGM), which can vary from localized illness, such as skin disease, to disseminated disease. Amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem and sulfamethoxazole are antimicrobial drugs chosen to treat such illnesses; however, not all patients obtain the cure. The reason why the treatment does not work for those patients is related to the fact that some clinical strains present resistance to the existing antimicrobial drugs; thereby, the research of new therapeutic approaches is extremely relevant...
October 2016: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Vanessa da Costa Flores, Fallon Dos Santos Siqueira, Caren Rigon Mizdal, Pauline Cordenonsi Bonez, Vanessa Albertina Agertt, Sílvio Terra Stefanello, Grazielle Guidolin Rossi, Marli Matiko Anraku de Campos
Rapidly growing mycobacteria (RGM) are opportunistic pathogens found in the environment. When in biofilms, mycobacteria is highly resistant to antibacterial treatments. The purpose of this study is to evaluate the antibiofilm activity of antimicrobials commonly used in therapy against mycobacteria. The antimicrobial susceptibility of Mycobacterium abscessus, Mycobacterium fortuitum and Mycobacterium massiliense was determined in planktonic and sessile populations. The antimicrobials amikacin, ciprofloxacin, clarithromycin, doxycycline, imipenem and sulfamethoxazole were tested...
October 2016: Microbial Pathogenesis
Asuman Şamlı, Arzu İlki
Mycobacteria are an important cause of morbidity in humans. Rapid and accurate mycobacterial identification is important for improving patient outcomes. However, identification of Mycobacterium species is not easy, due to the slow and fastidious growth of mycobacteria. Recently, biochemical, sequencing, and probing methods have come to be used for identification. This study compared the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of M...
August 23, 2016: New Microbiologica
Yoshiya Tsunoda, Akimasa Sekine, Yuki Sumazaki, Toru Tanaka, Shih-Yuan Lin, Hiroyuki Takoi, Kenji Hayashihara, Ataru Moriya, Takefumi Saito
INTRODUCTION: Mycobacterium abscessus pulmonary disease is common in patients with bronchiectasis. However, the underlying disease that is more likely to be present in patients with M. abscessus pulmonary disease remains poorly understood. METHOD: From 2001 through 2010, all patients, whose sputum or bronchoscopic lavage cultures yielded M. abscessus, were included in the study. RESULTS: Among the 11 patients included (male/female: 4/7), 4 male patients had a history of smoking...
April 2016: Kekkaku: [Tuberculosis]
Thaís Guimarães, Erica Chimara, Gladys Villas Boas do Prado, Lucilaine Ferrazoli, Natalia Garcia Fernandes Carvalho, Fernanda Cristina Dos Santos Simeão, Andréia Rodrigues de Souza, Christiane A R Costa, Cristina Viana Niero, Urze Adomaitis Brianesi, Thais Romano di Gioia, Laura Maria Brasileiro Gomes, Fernanda de Souza Spadão, Maria das Graças Silva, Eduardo Guimarães Hourneaux de Moura, Anna S Levin
BACKGROUND: The nontuberculous mycobacteria (NTM) are widely spread. In Brazil, 2,520 cases of rapidly growing mycobacteria (RGM) infections after medical procedures were reported, with 5.4% of cases related to nonsurgical invasive procedures and with an occurrence of 1 clone (BRA100) of Mycobacterium abscessus subsp bolletii. OBJECTIVE: To describe a pseudooutbreak of M abscessus subsp bolletii in an endoscopy and bronchoscopy unit. METHODS: The alert for a pseudooutbreak was given when 3 patients, in the same week, had a positive bronchoalveolar lavage culture for M abscessus subsp bolletii...
August 11, 2016: American Journal of Infection Control
Nurul Yaqeen Mohd Esa, Adli Azam Mohd Radzi, Nor Salmah Bakar, Mohd Shukry Mohd Khalid, Ahmad Izuanuddin Ismail, Mohamed Fauzi Abdul Rani
Teratomas of anterior mediastinum are rare. They are often slow growing, asymptomatic, and detected incidentally on chest imaging. Mycobacterium abscessus (M. abscessus) is an acid-fast bacillus that is classified as a pathogenic "rapid growing" non-tuberculous mycobacteria. It is an uncommon cause of human pathology, which may cause skin and soft tissue infection after skin injury following inoculation, minor trauma, and surgery. Here, we present an unusual case of benign cystic teratoma mimicking recurrent pleural effusion, which was subsequently complicated by M...
May 2016: Respirology Case Reports
H Y Kim, S Y Lee, B J Kim, Y H Kook
On the basis of the structural differences of erm, we used a duplex polymerase chain reaction (PCR) to differentiate Mycobacterium abscessus subsp. abscessus and subsp. massiliense isolates and to detect the point mutations of 23S rRNA gene that confer a high level of resistance to clarithromycin. Subsp. massiliense strains occupying almost half of the clinical isolates can be simply identified, and their clarithromycin susceptibility can be rapidly determined.
July 2016: Indian Journal of Medical Microbiology
Albertus Viljoen, Mickael Blaise, Chantal de Chastellier, Laurent Kremer
Slow growing pathogenic mycobacteria utilize host-derived lipids and accumulate large amounts of triacylglycerol (TAG) in the form of intracytoplasmic lipid inclusions (ILI), serving as a source of carbon and energy during prolonged infection. Mycobacterium abscessus is an emerging and rapidly growing species capable to induce severe and chronic pulmonary infections. However, whether M. abscessus, like Mycobacterium tuberculosis, possesses the machinery to acquire and store host lipids, remains unaddressed...
August 11, 2016: Molecular Microbiology
Ho Namkoong, Kozo Morimoto, Tomoyasu Nishimura, Hiromu Tanaka, Hiroaki Sugiura, Yoshitake Yamada, Atsuko Kurosaki, Takanori Asakura, Shoji Suzuki, Hiroshi Fujiwara, Kazuma Yagi, Makoto Ishii, Sadatomo Tasaka, Tomoko Betsuyaku, Yoshihiko Hoshino, Atsuyuki Kurashima, Naoki Hasegawa
BACKGROUND: Mycobacterium abscessus (M. abscessus) pulmonary disease is a refractory chronic infectious disease. Options for treating M. abscessus pulmonary disease are limited, especially in outpatient settings. Among parenteral antibiotics against M. abscessus, intravenous amikacin (AMK) is expected to be an effective outpatient antimicrobial therapy. This study evaluated the clinical efficacy and safety of intravenous AMK therapy in outpatients with M. abscessus pulmonary disease. METHODS: This retrospective chart review of cases of M...
2016: BMC Infectious Diseases
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