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https://www.readbyqxmd.com/read/29668016/genetic-and-ecological-niche-modeling-of-calydorea-crocoides-iridaceae-an-endemic-species-of-subtropical-highland-grasslands
#1
Juliana Lustosa Matos de Alencar, Eliane Kaltchuk-Santos, Juliana Fachinetto, Luana Olinda Tacuatiá, Eliana Regina Forni-Martins, Eudes Maria Stiehl-Alves, Tatiana Teixeira de Souza-Chies
Evolutionary analyses have been widely used for evaluation of genetic diversity of natural populations and correlate these data to the fitness of the species, especially in the case of threatened species. Calydorea crocoides occurs in a restricted area at altitudes from 800 to 1500 m in southern Brazil and is considered endangered. A study assessing genetic diversity, cytogenetic features and ecological niche was performed aiming to characterize C. crocoides by multidisciplinary approaches. Molecular data highlighted that most of the total variation (76%; p < 0...
2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29667882/new-doppler-imaging-technique-for-assessing-angiogenesis-in-breast-tumors-correlation-with-immunohistochemically-analyzed-microvessels-density
#2
Sewha Kim, Hye Jin Lee, Kyung Hee Ko, Ah Young Park, Jieun Koh, Hae Kyoung Jung
Background Microvessel density (MVD) is associated with grade and prognosis in breast tumors. However, conventional color Doppler flow (CDF) imaging has been limited to represent MVD of breast tumors. Purpose To evaluate whether a new Doppler imaging technique (AngioPLUS) can represent MVD of breast tumors. Material and Methods The institutional review board approved this retrospective study, and patients' informed consent was waived. CDF and AngioPLUS were available in pathologically confirmed 55 breast tumors of 53 women...
January 1, 2018: Acta Radiologica
https://www.readbyqxmd.com/read/29667809/strand-displacement-probes-combined-with-isothermal-nucleic-acid-amplification-for-instrument-free-detection-from-complex-samples
#3
Elizabeth A Phillips, Taylor J Moehling, Sanchita Bhadra, Andrew D Ellington, Jacqueline C Linnes
Sensitive and specific detection of pathogens via nucleic acid amplification is currently constrained to laboratory settings and portable equipment with costly fluorescent detectors. Nucleic acid-detecting lateral flow immunoassay strips (LFIAs) offer a low-cost visual transduction strategy at points of need. Unfortunately, these LFIAs frequently detect amplification byproducts that can yield spurious results which can only be deciphered through statistical analysis. We integrated customizable strand displacement probes into standard loop mediated isothermal amplification (LAMP) assays to prevent byproduct capture on commercial LFIAs...
April 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29667515/preparation-and-characterisation-of-activated-carbon-from-waste-tea-by-physical-activation-using-steam
#4
Jiazhen Zhou, Anran Luo, Youcai Zhao
In this study, the feasibility of preparing activated carbon from waste tea by physical activation using steam was investigated. The effects of activation temperature on yield and pore properties of the prepared activated carbon were studied. The yield decreased with increased activation temperature owing to the decomposition of cellulose and hemicellulose. The specific surface area and pore volume of the activated carbon were estimated using the Brunauer-Emmett-Teller method, Langmuir equation, and t-plot method...
April 18, 2018: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/29667269/effects-of-bmp-2-on-the-osteogenic-differentiation-of-bone-marrow-stem-cells-in-fibrous-dysplasia
#5
Soyoun Um, Hui Young Kim, Byoung-Moo Seo
OBJECTIVES: The purpose of this study was to evaluate the effects of BMP-2 on bone-grafting procedures for treatment of fibrous dysplasia based on in vitro and in vivo experiments. SUBJECTS AND METHODS: Proliferation of stem cells was determined by colony-forming assay, CCK-8 assay, and BrdU staining. OCT4 and NANOG expression was analyzed by flow cytometry and immunocytochemistry. Osteogenic differentiation was assessed by measuring ALP activity, alizarin red S staining, and in vivo transplantation...
April 18, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29667051/performance-assessment-of-laboratory-and-field-scale-multi-step-passive-treatment-of-iron-rich-acid-mine-drainage-for-design-improvement
#6
Tsiverihasina V Rakotonimaro, Carmen Mihaela Neculita, Bruno Bussière, Thomas Genty, Gérald J Zagury
Multi-step passive systems for the treatment of iron-rich acid mine drainage (Fe-rich AMD) perform satisfactorily at the laboratory scale. However, their field-scale application has revealed dissimilarities in performance, particularly with respect to hydraulic parameters. In this study, the assessment of factors potentially responsible for the variations in performance of laboratory and field-scale multi-step systems was undertaken. Three laboratory multi-step treatment scenarios, involving a combination of dispersed alkaline substrate (DAS) units, anoxic dolomitic drains, and passive biochemical reactors (PBRs), were set up in 10...
April 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29667025/biosorption-of-metal-ions-on-lignocellulosic-materials-batch-and-continuous-flow-process-studies
#7
Muhammad Mahmood-Ul-Hassan, Vishandas Suthar, Rizwan Ahmad, Munazza Yousra
Metal adsorption capacities of bioadsorbents, derived from low-cost agricultural waste, were assessed. Batch and column experiments were conducted for evaluation of lead (Pb), cadmium (Cd), and chromium (Cr) sorption kinetics on to modified (by treating with base and acid) and unmodified sugarcane bagasse and corn cob. Langmuir, Freundlich, and Redlich-Peterson equations were used to understand metal adsorption behavior and Elovich and Lagergren's pseudo-first-order and pseudo-second-order kinetics equations were used for estimation of adsorption kinetics parameter...
April 17, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29666882/river-flow-impacts-bacterial-and-archaeal-community-structure-in-surface-sediments-in-the-northern-gulf-of-mexico
#8
Alice C Ortmann, Pamela M Brannock, Lei Wang, Kenneth M Halanych
Meiobenthic community structure in the northern Gulf of Mexico has been shown to be driven by geographical differences due to inshore-offshore gradients and location relative to river discharge. Samples collected along three transects spanning Mobile Bay, Alabama, showed significant differences in meiobenthic communities east of the bay compared to those sampled from the west. In contrast, analysis of bacterial and archaeal communities from the same sediment samples shows that the inshore-offshore gradient has minimal impact on their community structure...
April 17, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29666881/pathological-factors-contributing-to-crossed-cerebellar-diaschisis-in-cerebral-gliomas-a-study-combining-perfusion-diffusion-and-structural-mr-imaging
#9
Xiaoxue Liu, Jianrui Li, Qiang Xu, Dante Mantini, Peng Wang, Yuan Xie, Yifei Weng, Chiyuan Ma, Kangjian Sun, Zhiqiang Zhang, Guangming Lu
PURPOSE: To investigate imaging features of crossed cerebellar diaschisis (CCD) in cerebral gliomas, and its underlying pathophysiological mechanisms. METHODS: Thirty-three pre-surgical patients with cerebral gliomas and 33 healthy controls underwent arterial spin-labeling, diffusion tensor imaging, and high-resolution T1-weighted imaging using MRI, in order to estimate cerebral blood flow (CBF), white matter integrity, and lesion volume, respectively. Asymmetry indices of CBF in the cerebellum were used for evaluating the level of CCD in the patients...
April 17, 2018: Neuroradiology
https://www.readbyqxmd.com/read/29666301/fratricide-of-nk-cells-in-daratumumab-therapy-for-multiple-myeloma-overcome-by-ex-vivo-expanded-autologous-nk-cells
#10
Yufeng Wang, Yibo Zhang, Tiffany Hughes, Jianying Zhang, Michael A Caligiuri, Don M Benson, Jianhua Yu
PURPOSE: Daratumumab and its use in combination with other agents is becoming a new standard of care for treatment of multiple myeloma (MM). We mechanistically studied how daratumumab acts on NK cells. EXPERIMENTAL DESIGN: Quantities of NK cells in peripheral blood (PB) and/or bone marrow (BM) of MM patients or healthy donors were examined by flow cytometry. NK cell apoptosis and the associated mechanism were assessed by flow cytometry and immunoblotting. Patients' NK cells were expanded in vitro using feeder cells...
April 17, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29666293/fast-or-slow-rescue-ventilations-a-predictive-model-of-gastric-inflation
#11
John R Fitz-Clarke
BACKGROUND: Rescue ventilations are given during respiratory and cardiac arrest. Tidal volume must assure oxygen delivery; however, excessive pressure applied to an unprotected airway can cause gastric inflation, regurgitation, and pulmonary aspiration. The optimal technique provides mouth pressure and breath duration that minimize gastric inflation. It remains unclear if breath delivery should be fast or slow, and how inflation time affects the division of gas flow between the lungs and esophagus...
April 17, 2018: Respiratory Care
https://www.readbyqxmd.com/read/29666009/in-vitro-validation-of-a-self-driving-aortic-turbine-venous-assist-device-for-fontan-patients
#12
Kerem Pekkan, Ibrahim Basar Aka, Ece Tutsak, Erhan Ermek, Haldun Balim, Ismail Lazoglu, Riza Turkoz
BACKGROUND: Palliative repair of single ventricle defects involve a series of open-heart surgeries where a single-ventricle (Fontan) circulation is established. As the patient ages, this paradoxical circulation gradually fails, because of its high venous pressure levels. Reversal of the Fontan paradox requires an extra subpulmonic energy that can be provided through mechanical assist devices. The objective of this study was to evaluate the hemodynamic performance of a totally implantable integrated aortic-turbine venous-assist (iATVA) system, which does not need an external drive power and maintains low venous pressure chronically, for the Fontan circulation...
March 11, 2018: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29665897/-effects-of-triptolide-combined-with-homoharringtonine-on-proliferation-and-apoptosis-of-kg-1%C3%AE-cells
#13
Xin Li, Jia-Yan Liu, Xiao-Hong Yuan, Zhen-Xing Lin, Yong Wu
OBJECTIVE: To investigate the effect and possible mechanism of low concentration of triptolide (TPL) combined with homoharringtonine (HHT) on the proliferation and apoptosis of KG-1α cells. METHODS: CCK-8 method was used to detect the antiproliferating effects of different concentrations of TPL and HHT single-use and combined use on KG-1α cells, and the combined index (CI) was calculated. The colony formation ability was also determined by methylcellulose colony formation assay, cell surface molecules, apoptosis rate and cell cycle changes were detected by flow cytometry...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665852/a-novel-method-for-demonstrating-cold-agglutinin-disease-a-case-report
#14
Thomas A Vo, Zack Oakey, Yasir A Khan, Donald S Minckler
BACKGROUND: Cold agglutinin disease is a rare disorder characterized by an autoimmune hemolytic anemia occurring at low temperatures. Physical examination findings, often limited to acrocyanosis, are combined with a thermal amplitude test to help establish the diagnosis. Thermal amplitude testing determines the highest temperature at which the cold agglutination will occur and is an important parameter in diagnosing cold agglutinin disease. CASE PRESENTATION: Here we describe a 57-year-old white man of German and Nicaraguan descent with known chronic cold agglutinin disease who presented to our ophthalmology clinic for evaluation of a cataract...
April 18, 2018: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/29665787/valproic-acid-sensitizes-metformin-resistant-human-renal-cell-carcinoma-cells-by-upregulating-h3-acetylation-and-emt-reversal
#15
Muyun Wei, Shaowei Mao, Guoliang Lu, Liang Li, Xiaopeng Lan, Zhongxian Huang, Yougen Chen, Miaoqing Zhao, Yueran Zhao, Qinghua Xia
BACKGROUND: Metformin (Met) is a widely available diabetic drug and shows suppressed effects on renal cell carcinoma (RCC) metabolism and proliferation. Laboratory studies in RCC suggested that metformin has remarkable antitumor activities and seems to be a potential antitumor drug. But the facts that metformin may be not effective in reducing the risk of RCC in cancer clinical trials made it difficult to determine the benefits of metformin in RCC prevention and treatment. The mechanisms underlying the different conclusions between laboratory experiments and clinical analysis remains unclear...
April 17, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29665685/dynamic-asphaltene-stearic-acid-competition-at-the-oil-water-interface
#16
Bastian Sauerer, Mikhail Stukan, Jan Buiting, Wael Abdallah, Simon Ivar Andersen
Interfacial tension (IFT) is one of the major parameters which govern the fluid flow in oil production and recovery. This paper investigates the interfacial activity of different natural surfactants found in crude oil. The main objective was to better understand the competition between carboxylic acids and asphaltenes on toluene/water interfaces. Dynamic IFT was measured for water-in-oil pendant drops contrary to most studies using oil-in-water drops. Stearic acid (SA) was used as model compound for surface-active carboxylic acids in crude...
April 17, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29665242/comparative-analysis-of-marketed-factor-viii-products-recombinant-products-are-not-alike-vis-a-vis-soluble-protein-aggregates-and-subvisible-particles
#17
J Anzengruber, C Lubich, T Prenninger, A Gringeri, F Scheiflinger, B M Reipert, M Malisauskas
BACKGROUND: Recombinant protein technologies have facilitated the development of novel FVIII therapeutics with improved production efficiency, potency, half-live, and low risk of viral transmission. The increasing number of recombinant Factor VIII (rFVIII) products and information on their efficacy, safety, and cost allow patients and healthcare professionals to adjust treatment to individual needs. Nonetheless, 20-32% of previously untreated patients with severe hemophilia A develop inhibitory antibodies to rFVIII following treatment...
April 17, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29665013/planhab-hypoxia-does-not-worsen-the-impairment-of-skeletal-muscle-oxidative-function-induced-by-bed-rest-alone
#18
Desy Salvadego, Michail E Keramidas, Roger Kölegård, Lorenza Brocca, Stefano Lazzer, Irene Mavelli, Jörn Rittweger, Ola Eiken, Igor B Mekjavic, Bruno Grassi
KEY POINTS: Superposition of hypoxia on 21 day bed rest did not worsen the impairment of skeletal muscle oxidative function induced by bed rest alone. A significant impairment of maximal oxidative performance was identified downstream of cardiovascular O2 delivery, involving both the intramuscular matching between O2 supply and utilization and mitochondrial respiration. These chronic adaptations appear to be relevant in terms of exposure to spaceflights and reduced gravity habitats (Moon or Mars), as characterized by low gravity and hypoxia, in patients with chronic diseases characterized by hypomobility/immobility and hypoxia, as well as in ageing...
April 17, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29664669/primary-nonsecretory-plasma-cell-leukemia-with-multiple-chromosomal-abnormalities-a-case-report
#19
Satoshi Nakayama, Tomohiro Oda, Shinya Minabe, Yukiharu Bamba, Haruko Ukawa, Rika Sato, Masaki Kawabata, Toshikatsu Horiuchi, Junichi Watanabe, Fumihiko Kimura, Hideyuki Shimazaki, Sho Ogata, Kuniaki Nakanishi
Primary nonsecretory plasma cell leukemia (PCL) is an extremely rare type of multiple myeloma. Here, we report a case of nonsecretory PCL with no previous history of multiple myeloma. The case exhibited extremely low levels of serum immunoglobulin and light chain, no detectable serum M-protein or free light chain restriction, no urine BJP, and no cytoplasmic light chain expression in flow cytometry. In fluorescence in situ hybridization, tumor cells exhibited fusion genes for IgH/BCL1 and IgH/cMyc, disappearance of the p53 signal, and a split signal for IgK(2p11), but no split signal for IgL (22q11)...
January 1, 2018: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/29664421/n-type-doping-and-morphology-of-gaas-nanowires-in-aerotaxy
#20
Wondwosen Metaferia, Sudhakar Sivakumar, Axel R Persson, Irene Geijselaers, L Reine Wallenberg, Knut Deppert, Lars Samuelson, Martin Magnusson
Controlled doping in semiconductor nanowires modifies their electrical and optical properties, which are important for high efficiency optoelectronic devices. We have grown n-type (Sn) doped GaAs nanowires in Aerotaxy, a new continuous gas phase mass production technique. The morphology of Sn doped nanowires is found to be a strong function of dopant, tetraethyltin to trimethylgallium flow ratio, Au-Ga-Sn alloying, and nanowire growth temperatures. High temperature and high flow ratios result in low morphological quality nanowires and in parasitic growth on the wire base and surface...
April 17, 2018: Nanotechnology
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