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https://www.readbyqxmd.com/read/28340420/extended-anaerobic-conditions-in-the-biological-wastewater-treatment-higher-reduction-of-toxicity-compared-to-target-organic-micropollutants
#1
Johannes Völker, Tobias Vogt, Sandro Castronovo, Arne Wick, Thomas A Ternes, Adriano Joss, Jörg Oehlmann, Martin Wagner
Extended anaerobic conditions during biological wastewater treatment may enhance the biodegradation of micropollutants. To explore this, we combined iron-reducing or substrate-limited anaerobic conditions and aerobic pilot-scale reactors directly at a wastewater treatment plant. To investigate the detoxification by these processes, we applied two in vitro bioassays for baseline toxicity (Microtox) and reactive toxicity (AREc32) as well as in vivo bioassays with aquatic model species in two laboratory experiments (Desmodesmus subspicatus, Daphnia magna) and two on-site, flow-through experiments (Potamopyrgus antipodarum, Lumbriculus variegatus)...
March 14, 2017: Water Research
https://www.readbyqxmd.com/read/28340307/genetic-diversity-analysis-of-asian-clam-corbicula-fluminea-in-the-hongze-lake-based-on-mitochondrial-cytochrome-b-gene
#2
Chuankun Zhu, Jin Li, Songguang Xie, Huaiyu Ding, Zhengjun Pan, Hui Wang, Guoliang Chang
The Asian clam Corbicula fluminea is a small bivalve with high nutritional and medical values. However, natural resources of C. fluminea have declined in many areas of China including the Hongze Lake. In this study, 119 individuals from 10 sites of this lake and 2 outgroups were analyzed using a 456 bp mitochondrial cytochrome b (cytb) gene segment. Totally, 19 polymorphic sites were detected, which defined 16 haplotypes. Polymorphism varied among the 10 populations with those at the water inlet being more polymorphic...
March 24, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28339192/a-novel-exposure-system-termed-navetta-for-in-vitro-laminar-flow-electrodeposition-of-nanoaerosol-and-evaluation-of-immune-effects-in-human-lung-reporter-cells
#3
Evelien Frijns, Sandra Verstraelen, Linda Corinna Stoehr, Jo van Laer, An Jacobs, Jan Peters, Kristof Tirez, Matthew Boyles, Mark Geppert, Pierre Madl, Inge Nelissen, Albert Duschl, Martin Himly
A new prototype air-liquid interface (ALI) exposure system, a flatbed aerosol exposure chamber termed NAVETTA, was developed to investigate deposition of engineered nanoparticles (NPs) on cultured human lung A549 cells directly from the gas phase. This device mimics human lung cell exposure to NPs due to a low horizontal gas flow combined with cells exposed at the ALI. Electrostatic field assistance is applied to improve NP deposition efficiency. As proof-of-principle, cell viability and immune responses after short-term exposure to nano-copper oxide (CuO)-aerosol were determined...
March 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28339016/paeonol-ameliorates-imiquimod-induced-psoriasis-like-skin-lesions-in-balb-c-mice-by-inhibiting-the-maturation-and-activation-of-dendritic-cells
#4
Yujiao Meng, Mingxing Wang, Xiangjiang Xie, Tingting Di, Jingxia Zhao, Yan Lin, Xiaolong Xu, Ningfei Li, Yating Zhai, Yan Wang, Ping Li
Paeonol, an active component derived from the traditional Chinese medicine Cortex Moutan, possesses anti-inflammatory, analgesic, antioxidant and anti-allergic properties. Psoriasis is a chronic, recurrent, inflammatory dermatosis accompanied by excessive activation of Toll‑like receptors (TLRs) in dendritic cells (DCs), which are primarily responsible for initiating an immune response. We investigated the effect of paeonol on inflammation in an imiquimod (IMQ)-induced psoriasis-like mouse model and murine bone marrow-derived dendritic cells (BMDCs) stimulated by R848...
March 21, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28338198/the-expression-of-sirt3-in-primary-hepatocellular-carcinoma-and-the-mechanism-of-its-tumor-suppressing-effects
#5
Y Liu, Y-L Liu, W Cheng, X-M Yin, B Jiang
OBJECTIVE: To observe the SIRT 3 expression in primary hepatocellular carcinoma (HCC), and then establish the eukaryotic expression vector of SIRT3 to observe the proliferation and apoptosis of pZsGreen-c1-SIRT3 HepG2 cells. Furthermore, we explored the mechanism of SIRT3 in inhibiting HCC. PATIENTS AND METHODS: Immunohistochemistry was used to detect the expression of SIRT3 in the tumor tissue and para-tumor tissue in 32 patients with HCC and the normal liver tissue in 10 patients...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28338196/study-on-the-proliferation-of-human-gastric-cancer-cell-ags-by-activation-of-egfr-in-h2o2
#6
Q Wang, W Shen, G-Q Tao, J Sun, L-P Shi
OBJECTIVE: This study is to investigate the effect of low concentration hydrogen peroxide (H2O2) on the proliferation of gastric cancer AGS cell line in vitro and the mechanism. MATERIALS AND METHODS: AGS cells were treated with different low concentrations of H2O2 (1, 0.1, 0.01, and 0.001 μm) for 48 hours. The effect of H2O2 concentration gradient on the activity of AGS cell activities was detected by methyl thiazolyl tetrazolium (MTT) method. The expression of the epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2 was detected by Western blot method; moreover, the effect of H2O2 on intracellular reactive oxygen species (ROS) in AGS cells was observed under the fluorescence microscope and quantitative analysis by flow cytometry...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28337914/electrically-actuated-valves-for-woven-fabric-lateral-flow-devices
#7
Tanya Narahari, Dhananjaya Dendukuri, Shashi K Murthy
The integration of flow control elements into low-cost biosensors, presents a significant engineering challenge. This article describes the development and integration of active, chemical valves into lateral flow devices, using a scalable, single-step, weaving-based manufacturing approach. The valve was constructed from an electrically conductive polymer, polypyrrole. The polymer switches between wetting and non-wetting states when it is reduced and oxidized via the application of an electrochemical potential...
March 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28337628/study-of-plasma-off-gas-treatment-from-spent-ion-exchange-resin-pyrolysis
#8
Hernán Ariel Castro, Vittorio Luca, Hugo Luis Banchi
Polystyrene divinylbenzene-based ion exchange resins are employed extensively within nuclear power plants (NPPs) and research reactors for purification and chemical control of the cooling water system. To maintain the highest possible water quality, the resins are regularly replaced as they become contaminated with a range of isotopes derived from compromised fuel elements as well as corrosion and activation products including (14)C, (60)Co, (90)Sr, (129)I, and (137)Cs. Such spent resins constitute a major proportion (in volume terms) of the solid radioactive waste generated by the nuclear industry...
March 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28337541/tyrosine-kinase-inhibitor-therapy-induced-changes-in-humoral-immunity-in-patients-with-chronic-myeloid-leukemia
#9
Hanna L M Rajala, Mohamed El Missiry, Anniina Ruusila, Perttu Koskenvesa, Tim H Brümmendorf, Bjorn T Gjertsen, Jeroen Janssen, Kourosh Lotfi, Berit Markevärn, Ulla Olsson-Strömberg, Leif Stenke, Jesper Stentoft, Johan Richter, Henrik Hjorth-Hansen, Anna Kreutzman, Satu Mustjoki
PURPOSE: Tyrosine kinase inhibitors (TKIs) have well-characterized immunomodulatory effects on T and NK cells, but the effects on the humoral immunity are less well known. In this project, we studied TKI-induced changes in B cell-mediated immunity. METHODS: We collected peripheral blood (PB) and bone marrow (BM) samples from chronic myeloid leukemia (CML) patients before and during first-line imatinib (n = 20), dasatinib (n = 16), nilotinib (n = 8), and bosutinib (n = 12) treatment...
March 23, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28337415/a-modification-of-the-constant-head-permeameter-to-measure-saturated-hydraulic-conductivity-of-highly-permeable-media
#10
Jelmer J Nijp, Klaas Metselaar, Juul Limpens, Harm P A Gooren, Sjoerd E A T M van der Zee
The saturated hydraulic conductivity (Ks ) is a key characteristic of porous media, describing the rate of water flow through saturated porous media. It is an indispensable parameter in a broad range of simulation models that quantify saturated and/or unsaturated water flow. The constant-head permeameter test is a common laboratory method to determine Ks on undisturbed soil samples collected from the field. In this paper we show that the application of this conventional method may result in a biased Ks in the case of highly permeable media, such as the top layer of Sphagnum peat and gravel...
2017: MethodsX
https://www.readbyqxmd.com/read/28336885/preparation-and-characterization-of-cellulose-nanocrystals-from-the-bio-ethanol-residuals
#11
Lanxing Du, Jinwu Wang, Yang Zhang, Chusheng Qi, Michael P Wolcott, Zhiming Yu
This study was to explore the conversion of low-cost bio-residuals into high value-added cellulose nanocrystals. Two enzymatic hydrolyzed residuals (i.e., HRMMW and HRSPW) were collected from two different bio-ethanol producing processes-hydrolyzing medium-milled wood (MMW) and hydrolyzing acid sulfite pretreated wood (SPW), respectively. The results showed that both residuals contained over 20 wt % glucan with a crystallinity of about 30%, confirming the existence of cellulose in a well-organized structure in two bio-residuals...
February 23, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28336765/mir-330-5p-suppresses-glioblastoma-cell-proliferation-and-invasiveness-through-targeting-itga5
#12
Linsen Feng, Jianhua Ma, Jing Ji, Haiming Ji, Yichun Liu, Weixing Hu
The present study intended to investigate the biological effects of miR-330-5p on glioblastoma (GBM) cell proliferation and invasiveness by targeting ITGA5. The expressions of miR-330-5p and ITGA5 mRNA in GBM cell lines (U87, U251 and U373) and normal brain glial cell line (HEB) were detected using RT-qPCR. Protein expression of ITGA5 was examined using Western blot.  This study used MTT assay, colony formation assay, Transwell assay, wound healing assay and flow cytometry analysis in order to determine the biological functions of GBM cells (including cell proliferation, invasion, migration, apoptosis and cell cycle)...
March 23, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28336114/paradoxical-aortic-stenosis-a-systematic-review
#13
Rita Cavaca, Rogério Teixeira, Maria João Vieira, Lino Gonçalves
Aortic stenosis (AS) is a complex systemic valvular and vascular disease with a high prevalence in developed countries. The new entity "paradoxical low-flow, low-gradient aortic stenosis" refers to cases in which patients have severe AS based on assessment of aortic valve area (AVA) (≤1 cm(2)) or indexed AVA (≤0.6 cm(2)/m(2)), but paradoxically have a low mean transvalvular gradient (<40 mmHg) and a low stroke volume index (≤35 ml/m(2)), despite preserved left ventricular ejection fraction (≥50%)...
March 20, 2017: Portuguese Journal of Cardiology: An Official Journal of the Portuguese Society of Cardiology
https://www.readbyqxmd.com/read/28335895/coronary-collaterals-function-and-clinical-outcome-between-patients-with-acute-and-chronic-total-occlusion
#14
Jang Hoon Lee, Chang-Yeon Kim, Namkyun Kim, Se Yong Jang, Myung Hwan Bae, Dong Heon Yang, Yongkeun Cho, Shung Chull Chae, Hun Sik Park
OBJECTIVES: This study sought to demonstrate how changes in the collateral function and its clinical significance before and after percutaneous coronary interventions (PCIs) are compared between patients with acute coronary syndrome and total or nearly total occlusions (ATOs) and chronic total occlusions (CTOs). BACKGROUND: The functional relevance of the collateral circulation in patients with ATOs and CTOs has not been fully investigated. METHODS: The pressure-derived collateral pressure index (CPI), myocardial fractional flow reserve (FFRmyo), and coronary fractional flow reserve (FFRcor) at maximum hyperemia induced by intravenous adenosine were evaluated in occluded vessels at baseline, after the PCI, and at 1 year in 23 ATO and 74 CTO patients...
March 27, 2017: JACC. Cardiovascular Interventions
https://www.readbyqxmd.com/read/28335769/cost-effectiveness-of-crag-lfa-screening-for-cryptococcal-meningitis-among-people-living-with-hiv-in-uganda
#15
Anu Ramachandran, Yukari Manabe, Radha Rajasingham, Maunank Shah
BACKGROUND: Cryptococcal meningitis (CM) constitutes a significant source of mortality in resource-limited regions. Cryptococcal antigen (CRAG) can be detected in the blood before onset of meningitis. We sought to determine the cost-effectiveness of implementing CRAG screening using the recently developed CRAG lateral flow assay in Uganda compared to current practice without screening. METHODS: A decision-analytic model was constructed to compare two strategies for cryptococcal prevention among people living with HIV with CD4 < 100 in Uganda: No cryptococcal screening vs...
March 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28335534/a-networked-sensor-system-for-the-analysis-of-plot-scale-hydrology
#16
German Villalba, Fernando Plaza, Xiaoyang Zhong, Tyler W Davis, Miguel Navarro, Yimei Li, Thomas A Slater, Yao Liang, Xu Liang
This study presents the latest updates to the Audubon Society of Western Pennsylvania (ASWP) testbed, a $50,000 USD, 104-node outdoor multi-hop wireless sensor network (WSN). The network collects environmental data from over 240 sensors, including the EC-5, MPS-1 and MPS-2 soil moisture and soil water potential sensors and self-made sap flow sensors, across a heterogeneous deployment comprised of MICAz, IRIS and TelosB wireless motes. A low-cost sensor board and software driver was developed for communicating with the analog and digital sensors...
March 20, 2017: Sensors
https://www.readbyqxmd.com/read/28335436/photonic-low-cost-micro-sensor-for-in-line-wear-particle-detection-in-flowing-lube-oils
#17
Jon Mabe, Joseba Zubia, Eneko Gorritxategi
The presence of microscopic particles in suspension in industrial fluids is often an early warning of latent or imminent failures in the equipment or processes where they are being used. This manuscript describes work undertaken to integrate different photonic principles with a micro- mechanical fluidic structure and an embedded processor to develop a fully autonomous wear debris sensor for in-line monitoring of industrial fluids. Lens-less microscopy, stroboscopic illumination, a CMOS imager and embedded machine vision technologies have been merged to develop a sensor solution that is able to detect and quantify the number and size of micrometric particles suspended in a continuous flow of a fluid...
March 14, 2017: Sensors
https://www.readbyqxmd.com/read/28335222/effects-of-atomization-injection-on-nanoparticle-processing-in-suspension-plasma-spray
#18
Hong-Bing Xiong, Cheng-Yu Zhang, Kai Zhang, Xue-Ming Shao
Liquid atomization is applied in nanostructure dense coating technology to inject suspended nano-size powder materials into a suspension plasma spray (SPS) torch. This paper presents the effects of the atomization parameters on the nanoparticle processing. A numerical model was developed to simulate the dynamic behaviors of the suspension droplets, the solid nanoparticles or agglomerates, as well as the interactions between them and the plasma gas. The plasma gas was calculated as compressible, multi-component, turbulent jet flow in Eulerian scheme...
May 20, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334286/differences-in-the-photosynthetic-plasticity-of-ferns-and-ginkgo-grown-in-experimentally-controlled-low-o2-co2-atmospheres-may-explain-their-contrasting-ecological-fate-across-the-triassic-jurassic-mass-extinction-boundary
#19
C Yiotis, C Evans-Fitz Gerald, J C McElwain
Background and Aims: Fluctuations in [CO 2 ] have been widely studied as a potential driver of plant evolution; however, the role of a fluctuating [O 2 ]:[CO 2 ] ratio is often overlooked. The present study aimed to investigate the inherent physiological plasticity of early diverging, extant species following acclimation to an atmosphere similar to that across the Triassic-Jurassic mass extinction interval (TJB, approx. 200 Mya), a time of major ecological change. Methods: Mature plants from two angiosperm ( Drimys winteri and Chloranthus oldhamii ), two monilophyte ( Osmunda claytoniana and Cyathea australis ) and one gymnosperm ( Ginkgo biloba ) species were grown for 2 months in replicated walk-in Conviron BDW40 chambers running at TJB treatment conditions of 16 % [O 2 ]-1900 ppm [CO 2 ] and ambient conditions of 21 % [O 2 ]-400 ppm [CO 2 ], and their physiological plasticity was assessed using gas exchange and chlorophyll fluorescence methods...
March 11, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28334280/origin-and-genetic-differentiation-of-pink-flowered-sorbus-hybrids-in-the-western-carpathians
#20
Veronika Uhrinová, Judita Zozomová-Lihová, Dana Bernátová, Juraj Paule, Ladislav Paule, Dušan Gömöry
Background and Aims: Diversity of the genus Sorbus has been affected by interspecific hybridizations. Pink-flowered hybrid species have been insufficiently studied so far. They comprise bigenomic hybrid species derived from crosses S. aria s.l. × S. chamaemespilus and trigenomic ones, where S. aucuparia was involved as well. The main objective of the present study was to reconstruct their hybrid origins as well as to assess genetic distinction among several morphologically recognized hybrid species...
March 13, 2017: Annals of Botany
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