keyword
MENU ▼
Read by QxMD icon Read
search

wilde aa

keyword
https://www.readbyqxmd.com/read/28549117/engineering-of-recombinant-wisteria-floribunda-agglutinin-specifically-binding-to-galnac%C3%AE-1-4glcnac-lacdinac
#1
Takashi Sato, Hiroaki Tateno, Hiroyuki Kaji, Yasunori Chiba, Tomomi Kubota, Jun Hirabayashi, Hisashi Narimatsu
Wisteria floribunda agglutinin (WFA) is a useful probe for distinguishing glycan structural alterations in diseases such as intrahepatic bile duct carcinoma and hepatic fibrosis; however, the gene encoding WFA has not been identified. Here, we identified the gene encoding WFA, and recombinant WFA was expressed in Escherichia coli and purified. The natural cDNA sequence obtained from wisteria seeds contained an open reading frame of 861 nucleotides encoding a WFA precursor, which included a hydrophobic signal peptide at the N-terminus, a propeptide at the C-terminus and a single cysteine (Cys) residue for dimer formation...
May 26, 2017: Glycobiology
https://www.readbyqxmd.com/read/28548828/screening-of-pro-asp-sequences-exposed-on-bacteriophage-m13-as-an-ideal-anchor-for-gold-nanocubes
#2
Hwa Kyoung Lee, Yujean Lee, Hyori Kim, Hye-Eun Lee, Hyejin Chang, Ki Tae Nam, Dae Hong Jeong, Junho Chung
Bacteriophages are thought to be ideal vehicles for linking antibodies to nanoparticles. Here, we define the sequence of peptides exposed as a fusion protein on M13 bacteriophages to yield optimal binding of gold nanocubes and efficient bacteriophage amplification. We generated five helper bacteriophage libraries using AE(X)2DP, AE(X)3DP, AE(X)4DP, AE(X)5DP, and AE(X)6DP as the exposed portion of pVIII, in which X was a randomized amino acid residue encoded by the nucleotide sequence NNK. Efficient phage amplification was achievable only in the AE(X)2DP, AE(X)3DP, and AE(X)4DP libraries...
May 26, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28546921/a-novel-frameshift-mutation-in-cx46-associated-with-hereditary-dominant-cataracts-in-a-chinese-family
#3
Xiu-Kun Cui, Ke-Ke Zhu, Zheng Zhou, Si-Min Wan, Yi Dong, Xuan-Ce Wang, Jing Li, Jing Zhang, Hong-Mei Mu, Lei Qin, Yan-Zhong Hu
AIM: To investigate the genetic mutations that are associated the hereditary autosomal dominant cataract in a Chinese family. METHODS: A Chinese family consisting of 20 cataract patients (including 9 male and 11 female) and 2 unaffected individuals from 5 generations were diagnosed to be a typical autosomal dominant cataract pedigree. Genomic DNA samples were extracted from the peripheral blood cells of the participants in this pedigree. Exon sequence was used for genetic mutation screening...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28545467/expression-of-deleted-atoxic-atypical-recombinant-beta2-toxin-in-a-baculovirus-system-and-production-of-polyclonal-and-monoclonal-antibodies
#4
Anna Serroni, Chiara Francesca Magistrali, Giovanni Pezzotti, Luca Bano, Martina Pellegrini, Giulio Severi, Chiara Di Pancrazio, Mirella Luciani, Manuela Tittarelli, Silvia Tofani, Antonio De Giuseppe
BACKGROUND: Clostridium perfringens is an important animal and human pathogen that can produce more than 16 different major and minor toxins. The beta-2 minor toxin (CPB2), comprising atypical and consensus variants, appears to be involved in both human and animal enterotoxaemia syndrome. The exact role of CPB2 in pathogenesis is poorly investigated, and its mechanism of action at the molecular level is still unknown because of the lack of specific reagents such as monoclonal antibodies against the CPB2 protein and/or the availability of a highly purified antigen...
May 25, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28545349/exploring-different-mutations-at-a-single-amino-acid-position-of-cucumber-green-mottle-mosaic-virus-replicase-to-attain-stable-symptom-attenuation
#5
Liming Liu, Bin Peng, Zhenwei Zhang, Yang Wu, Manuel Miras, Miguel A Aranda, Qinsheng Gu
Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus (family Virgaviridae) which causes serious economic losses in cucurbit crops. A possibility for CGMMV control is the use of cross-protection, for which stable attenuated isolates are required. In this study, an infectious clone was constructed for the hn isolate of CGMMV. Unexpectedly, this clone carried a non-conserved mutation involving a single nucleotide change resulting in the replacement of Arg to Cys at residue 284 of the replicase protein; this mutation correlated with delayed symptom induction and RNA accumulation, as shown in time course experiments...
May 25, 2017: Phytopathology
https://www.readbyqxmd.com/read/28545260/thermostabilization-of-the-uronate-dehydrogenase-from-agrobacterium-tumefaciens-by-semi-rational-design
#6
Teresa Roth, Barbara Beer, André Pick, Volker Sieber
Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natural resources such as seaweed or pectin as raw material. These contain large amounts of uronic acids as major constituents such as glucuronic acid and galacturonic acid which can be converted into the corresponding aldaric acids via an enzyme-based oxidation using uronate dehydrogenase (Udh)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28544674/direct-hydroxylation-of-benzene-to-phenol-by-cytochrome-p450bm3-triggered-by-amino-acid-derivatives
#7
Osami Shoji, Sota Yanagisawa, Joshua Kyle Stanfield, Kazuto Suzuki, Zhiqi Cong, Hiroshi Sugimoto, Yoshitsugu Shiro, Yoshihito Watanabe
Selective hydroxylation of benzene to phenol, without formation of side products resulting from overoxidation, was catalyzed by cytochrome P450BM3 (P450BM3) with the assistance of amino acid derivatives as decoy molecules. The catalytic turnover rate and the total turnover number reached 259 min-1P450BM3-1 and 40,200 P450BM3-1 when N-heptyl-L-proline modified with L-phenyl alanine (C7-L-Pro-L-Phe) was used as the decoy molecule. This work shows that amino acid derivatives that have a totally different structure from fatty acids can be used as decoy molecules for aromatic hydroxylation by wild-type P450BM3...
May 22, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28544059/objective-assessment-of-the-evolutionary-action-equation-for-the-fitness-effect-of-missense-mutations-across-cagi-blinded-contests
#8
Panagiotis Katsonis, Olivier Lichtarge
A major challenge in genome interpretation is to estimate the fitness effect of coding variants of unknown significance (VUS). Labor, limited understanding of protein functions, and lack of assays generally limit direct experimental assessment of VUS, and make robust and accurate computational approaches a necessity. Often, however, algorithms that predict mutational effect disagree amongst themselves and with experimental data, slowing their adoption for clinical diagnostics. To objectively assess such methods, the Critical Assessment of Genome Interpretation (CAGI) community organizes contests to predict unpublished experimental data, available only to CAGI assessors...
May 23, 2017: Human Mutation
https://www.readbyqxmd.com/read/28542638/a-single-amino-acid-mutation-affects-elicitor-and-expansins-like-activities-of-cerato-platanin-a-non-catalytic-fungal-protein
#9
Simone Luti, Federica Martellini, Francesco Bemporad, Lorenzo Mazzoli, Paolo Paoli, Luigia Pazzagli
Cerato-platanin (CP) is a non-catalytic, cysteine-rich protein, the first member of the cerato-platanin family. It is a single-domain protein with a double Ψ/β barrel domain resembling the D1 domain of plant and bacterial expansins. Similarly to expansins, CP shows a cell wall-loosening activity on cellulose and can be defined as an expanisin-like protein, in spite of the missing D2 domain, normally present in plant expansins. The weakening activity shown on cellulose may facilitate the CP-host interaction, corroborating the role of CP in eliciting plant defence response...
2017: PloS One
https://www.readbyqxmd.com/read/28539918/the-sapa-protein-is-involved-in-resistance-to-antimicrobial-peptide-pr-39-and-virulence-of-actinobacillus-pleuropneumoniae
#10
Fang Xie, Yalei Wang, Gang Li, Shuanghong Liu, Ning Cui, Siguo Liu, Paul R Langford, Chunlai Wang
Antimicrobial peptides are essential to the innate immune defense of the mammal against bacterial infection. However, pathogenic bacteria have evolved multiple strategies to resist and evade antimicrobial peptides, which is vital to bacterial survival and colonization in hosts. PR-39 is a linear porcine antimicrobial peptide containing 39 amino acid residues with a high proline content. Resistance to antimicrobial peptide PR-39 has been observed in Actinobacillus pleuropneumoniae. However, little is known about the factors required for this resistance...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28537245/-impact-of-mutations-in-nucleoprotein-on-replication-of-influenza-virus-a-hong-kong-1-68-162-35-reassortants-at-different-temperatures
#11
A A Pulkina, M V Sergeeva, S V Petrov, A V Fadeev, A B Komissarov, E A Romanovskaya-Romanko, M V Potapchuk, L M Tsybalova
The nucleoprotein (NP) of influenza virus is a multifunctional RNA binding protein. The role of NP in the adaptation of influenza viruses to a host has been experimentally proved. Ambiguous data are available on the role of nucleoprotein in the attenuation of influenza A viruses, which is characterized by ability to replicate at low temperature (26°C) and inability to replicate at high temperature (39°C). Influenza virus donor strain A/Hong Kong/1/68/162/35 (H3N2), adapted to growth at low temperature, differs from the wild type virus by 14 amino acid mutations in the internal and non-structural proteins...
March 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28536785/significance-of-accumulation-of-the-alarmone-p-ppgpp-in-chloroplasts-for-controlling-photosynthesis-and-metabolite-balance-during-nitrogen-starvation-in-arabidopsis
#12
Rina Honoki, Sumire Ono, Akira Oikawa, Kazuki Saito, Shinji Masuda
The regulatory nucleotides, guanosine 5'-triphosphate 3'-diphosphate (pppGpp) and guanosine 5'-diphosphate 3'-diphosphate (ppGpp), were originally identified in Escherichia coli, and control a large set of gene expression and enzyme activities. The (p)ppGpp-dependent control of cell activities is referred to as the stringent response. A growing number of (p)ppGpp synthase/hydrolase homologs have been identified in plants, which are localized in plastids in Arabidopsis thaliana. We recently reported that the Arabidopsis mutant overproducing ppGpp in plastids showed dwarf chloroplasts, and transcript levels in the mutant plastids were significantly suppressed...
May 23, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28536523/positive-modulatory-interactions-of-nmda-receptor-glun1-2b-ligand-binding-domains-attenuate-antagonists-activity
#13
Douglas Bledsoe, Ceyhun Tamer, Ivana Mesic, Christian Madry, Bradley G Klein, Bodo Laube, Blaise M Costa
N-methyl D-aspartate receptors (NMDAR) play crucial role in normal brain function and pathogenesis of neurodegenerative and psychiatric disorders. Functional tetra-heteromeric NMDAR contains two obligatory GluN1 subunits and two identical or different non-GluN1 subunits that include six different gene products; four GluN2 (A-D) and two GluN3 (A-B) subunits. The heterogeneity of subunit combination facilities the distinct function of NMDARs. All GluN subunits contain an extracellular N-terminal Domain (NTD) and ligand binding domain (LBD), transmembrane domain (TMD) and an intracellular C-terminal domain (CTD)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28536198/understanding-the-essential-proton-pumping-kinetic-gates-and-decoupling-mutations-in-cytochrome-c-oxidase
#14
Ruibin Liang, Jessica M J Swanson, Mårten Wikström, Gregory A Voth
Cytochrome c oxidase (CcO) catalyzes the reduction of oxygen to water and uses the released free energy to pump protons against the transmembrane proton gradient. To better understand the proton-pumping mechanism of the wild-type (WT) CcO, much attention has been given to the mutation of amino acid residues along the proton translocating D-channel that impair, and sometimes decouple, proton pumping from the chemical catalysis. Although their influence has been clearly demonstrated experimentally, the underlying molecular mechanisms of these mutants remain unknown...
May 23, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28536081/structural-annotation-of-beta-1-4-n-acetyl-galactosaminyltransferase-1-b4galnt1-causing-hereditary-spastic-paraplegia-26
#15
Rubina Dad, Uzma Malik, Aneela Javed, Berge A Minassian, Muhammad Jawad Hassan
Beta-1,4-N-acetyl galactosaminyltransferase 1, B4GALNT1, is a GM2/GD2 synthase, involved in the expression of glycosphingolipids (GSLs) containing sialic acid. Mutations in the gene B4GALNT1 cause Hereditary Spastic Paraplegia 26 (HSP26). In present study we have made attempt to predict the potential structural of the human B4GALNT1 protein. The results illustrated that the amino acid sequences of B4GALNT1 are not 100% conserved among selected twenty species. One signal peptide and one transmembrane domain predicted in human wild type B4GALNT1 protein with aliphatic index of 92...
May 20, 2017: Gene
https://www.readbyqxmd.com/read/28533942/hla-a24-ligandome-analysis-of-colon-and-lung-cancer-cells-identifies-a-novel-cancer-testis-antigen-and-a-neoantigen-that-elicits-specific-and-strong-ctl-responses
#16
Vitaly Kochin, Takayuki Kanaseki, Serina Tokita, Sho Miyamoto, Yosuke Shionoya, Yasuhiro Kikuchi, Daichi Morooka, Yoshihiko Hirohashi, Tomohide Tsukahara, Kazue Watanabe, Shingo Toji, Yasuo Kokai, Noriyuki Sato, Toshihiko Torigoe
This study focused on HLA-A24 and comprehensively analyzed the ligandome of colon and lung cancer cells without the use of MHC-binding in silico prediction algorithms. Affinity purification using the antibody specific to HLA-A24 followed by LC-MS/MS sequencing was used to detect peptides, which harbored the known characteristics of HLA-A24 peptides in terms of length and anchor motifs. Ligandome analysis demonstrated the natural presentation of two different types of novel tumor-associated antigens. The ligandome contained a peptide derived from SUV39H2, a gene found to be expressed in a variety of cancers but not in normal tissues (except for the testis)...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28533472/yersinia-pestis-yopk-inhibits-bacterial-adhesion-to-host-cells-by-binding-to-the-extracellular-matrix-adaptor-protein-matrilin-2
#17
Yafang Tan, Wanbing Liu, Qingwen Zhang, Shiyang Cao, Haihong Zhao, Tong Wang, Zhizhen Qi, Yanping Han, Yajun Song, Xiaoyi Wang, Ruifu Yang, Zongmin Du
Pathogenic yersiniae harbor a type III secretion system (T3SS) that injects Yersinia outer protein (Yop) into host cells. YopK has been shown to control Yop translocation and prevent inflammasome recognition of T3SS by the innate immune system. Here, we demonstrate that YopK inhibits bacterial adherence to host cells by binding to the extracellular matrix adaptor protein matrilin-2 (MATN2). YopK binds to MATN2 and deleting the amino acids 91--124 disrupts binding of YopK to MATN2. A yopK null mutant exhibits hyper-adhesive phenotype, which could be responsible for the established Yop hyper-translocation phenotype of yopK mutant...
May 22, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28531275/molecular-envelope-and-atomic-model-of-an-anti-terminated-glyqs-t-box-regulator-in-complex-with-trnagly
#18
Bhaskar Chetnani, Alfonso Mondragón
A T-box regulator or riboswitch actively monitors the levels of charged/uncharged tRNA and participates in amino acid homeostasis by regulating genes involved in their utilization or biosynthesis. It has an aptamer domain for cognate tRNA recognition and an expression platform to sense the charge state and modulate gene expression. These two conserved domains are connected by a variable linker that harbors additional secondary structural elements, such as Stem III. The structural basis for specific tRNA binding is known, but the structural basis for charge sensing and the role of other elements remains elusive...
May 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28531234/directed-evolution-to-improve-the-catalytic-efficiency-of-urate-oxidase-from-bacillus-subtilis
#19
Wenjie Li, Shouteng Xu, Biao Zhang, Yelin Zhu, Yan Hua, Xin Kong, Lianhong Sun, Jiong Hong
Urate oxidase is a key enzyme in purine metabolism and catalyzes the oxidation of uric acid to allantoin. It is used to treat hyperuricemia and gout, and also in a diagnostic kit. In this study, error-prone polymerase chain reaction and staggered extension process was used to generate a mutant urate oxidase with improved enzyme activity from Bacillus subtilis. After several rounds of mutagenesis and screening, two mutants 6E9 and 8E279 were obtained which exhibited 2.99 and 3.43 times higher catalytic efficiency, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28530581/metabolic-phenotyping-of-various-tea-camellia-sinensis-l-cultivars-and-understanding-of-their-intrinsic-metabolism
#20
Hyang-Gi Ji, Yeong-Ran Lee, Min-Seuk Lee, Kyeong Hwan Hwang, Eun-Hee Kim, Jun Seong Park, Young-Shick Hong
Recently, we selected three tea (Camellia sinensis) cultivars that are rich in taste, epigallocatechin-3-O-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG3″Me) and then cultivated them through asexual propagation by cutting in the same region. In the present study, proton nuclear magnetic resonance ((1)H NMR)-based metabolomics was applied to characterize the metabotype and to understand the metabolic mechanism of these tea cultivars including wild type tea. Of the tea leaf metabolite variations, reverse associations of amino acid metabolism with catechin compound metabolism were found in the rich-taste, and EGCG- and EGCG3″Me-rich tea cultivars...
October 15, 2017: Food Chemistry
keyword
keyword
98485
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"