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https://www.readbyqxmd.com/read/29146974/human-cyclophilin-b-forms-part-of-a-multi-protein-complex-during-erythrocyte-invasion-by-plasmodium-falciparum
#1
Prem Prakash, Mohammad Zeeshan, Ekta Saini, Azhar Muneer, Sachin Khurana, Bishwanath Kumar Chourasia, Arunaditya Deshmukh, Inderjeet Kaur, Surabhi Dabral, Niharika Singh, Zille Anam, Ayushi Chaurasiya, Shikha Kaushik, Pradeep Dahiya, Md Kalamuddin, Jitendra Kumar Thakur, Asif Mohmmed, Anand Ranganathan, Pawan Malhotra
Invasion of human erythrocytes by Plasmodium falciparum merozoites involves multiple interactions between host receptors and their merozoite ligands. Here we report human Cyclophilin B as a receptor for PfRhopH3 during merozoite invasion. Localization and binding studies show that Cyclophilin B is present on the erythrocytes and binds strongly to merozoites. We demonstrate that PfRhopH3 binds to the RBCs and their treatment with Cyclosporin A prevents merozoite invasion. We also show a multi-protein complex involving Cyclophilin B and Basigin, as well as PfRhopH3 and PfRh5 that aids the invasion...
November 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29144123/the-sign-of-nmr-chemical-shift-difference-as-a-determinant-of-the-origin-of-binding-selectivity-elucidation-of-the-position-dependence-of-phosphorylation-in-ligands-binding-to-scribble-pdz1
#2
Gustav Sundell, Beat Vögeli, Ylva Ivarsson, Celestine Chi
The use of NMR chemical shift perturbation to monitor changes taking place around the binding site of a ligand-protein interaction is a routine and widely applied methodology in the field of protein biochemistry. Shifts are often acquired by titrating various concentrations of ligand to a fixed concentration of the receptor and may serve the purposes, amongst others, to determine affinity constants, locate binding surfaces, or differentiate between binding mechanisms. Shifts are quantified by the so-called combined chemical shift difference...
November 16, 2017: Biochemistry
https://www.readbyqxmd.com/read/29128880/the-immunophilin-repertoire-of-plasmodiophora-brassicae-and-functional-analysis-of-pbcyp3-cyclophilin
#3
Khushwant Singh, Georgios Tzelepis, Miloslav Zouhar, Pavel Ryšánek, Christina Dixelius
Plasmodiophora brassicae is a soil-borne pathogen that belongs to Rhizaria, an almost unexplored eukaryotic organism group. This pathogen requires a living host for growth and multiplication, which makes molecular analysis further complicated. To broaden our understanding of a plasmodiophorid such as P. brassicae, we here chose to study immunophilins, a group of proteins known to have various cellular functions, including involvement in plant defense and pathogen virulence. Searches in the P. brassicae genome resulted in 20 putative immunophilins comprising of 11 cyclophilins (CYPs), 7 FK506-binding proteins (FKBPs) and 2 parvulin-like proteins...
November 11, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29127190/vesicle-mediated-secretion-of-misfolded-prion-protein-molecules-from-cyclosporin-a-treated-cells
#4
Ieshita Pan, Noa Roitenberg, Ehud Cohen
Loss of protein homeostasis is a hazardous situation that jeopardizes cellular functionality and viability. Cells have developed mechanisms that supervise protein integrity and direct misfolded molecules for degradation. Nevertheless, subsets of aggregation-prone proteins escape degradation and form aggregates that can underlie the development of neurodegenerative disorders. In some cases, cells deposit hazardous protein aggregates in designated sites, like aggresomes, or secrete them with vesicles. The prion protein (PrP) is an aggregation-prone, membrane-anchored glycoprotein, whose aggregation causes familial and sporadic, fatal, neurodegenerative diseases...
November 10, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29114388/epistatic-interactions-between-mutations-of-taci-tnfrsf13b-and-tcf3-result-in-a-severe-primary-immunodeficiency-disorder-and-systemic-lupus-erythematosus
#5
Rohan Ameratunga, Wikke Koopmans, See-Tarn Woon, Euphemia Leung, Klaus Lehnert, Charlotte A Slade, Jessica C Tempany, Anselm Enders, Richard Steele, Peter Browett, Philip D Hodgkin, Vanessa L Bryant
Common variable immunodeficiency disorders (CVID) are a group of primary immunodeficiencies where monogenetic causes account for only a fraction of cases. On this evidence, CVID is potentially polygenic and epistatic although there are, as yet, no examples to support this hypothesis. We have identified a non-consanguineous family, who carry the C104R (c.310T>C) mutation of the Transmembrane Activator Calcium-modulator and cyclophilin ligand Interactor (TACI, TNFRSF13B) gene. Variants in TNFRSF13B/TACI are identified in up to 10% of CVID patients, and are associated with, but not solely causative of CVID...
October 2017: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/29110442/cyclophilin-a-promotes-non-small-cell-lung-cancer-metastasis-via-p38-mapk
#6
Yinan Guo, Mei Jiang, Xiaoting Zhao, Meng Gu, Ziyu Wang, Shaofa Xu, Wentao Yue
BACKGROUND: Cyclophilin A (CypA) is associated with metastasis in diverse cancers; however, its role in lung cancer metastasis and the underlying mechanisms remain poorly understood. Our study investigated the effect of CypA on non-small cell lung cancer (NSCLC) metastasis in vitro and in vivo to determine its mechanisms. METHODS: In this study, A549 and H1299 cell lines with downregulated and overexpressed CypA, respectively, were constructed by lentivirus transfection of NSCLC cells...
November 7, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/29101324/cyclophilin-d-regulates-the-dynamic-assembly-of-mitochondrial-atp-synthase-into-synthasomes
#7
Gisela Beutner, Ryan E Alanzalon, George A Porter
Mitochondrial electron transport is essential for oxidative phosphorylation (OXPHOS). Electron transport chain (ETC) activity generates an electrochemical gradient that is used by the ATP synthase to make ATP. ATP synthase is organized into supramolecular units called synthasomes that increase the efficiency of ATP production, while within ATP synthase is the cyclophilin D (CypD) regulated mitochondrial permeability transition pore (PTP). We investigated whether synthasomes are dynamic structures that respond to metabolic demands and whether CypD regulates this dynamic...
November 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29097323/molecular-identification-and-expression-analysis-of-a-novel-cyclophilin-a-gene-in-the-red-swamp-crayfish-procambarus-clarkii
#8
Junjie Zhu, Feng Lin, Fei Li, Yan Wang
Cyclophilin A (Cyp A) is the main intracellular receptor of cyclosporin A (CsA) belonging to the immunophilin family, which is known as an effective immunosuppressive drug. This study aimed to gain insights into the structure and biological function of cyclophilin A in the red swamp crayfish, Procambarus clarkii (PcCypA). We cloned PcCypA by homology cloning and anchored polymerase chain reaction (PCR), and assessed its mRNA and protein expression levels in different tissues using quantitative real-time PCR and western blot analysis, respectively...
October 31, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29091948/selection-and-validation-of-appropriate-reference-genes-for-quantitative-real-time-pcr-analysis-in-salvia-hispanica
#9
Rahul Gopalam, Sunny D Rupwate, Ajay W Tumaney
Quantitative real-time polymerase chain reaction (qRT-PCR) has become the most popular choice for gene expression studies. For accurate expression analysis, it is pertinent to select a stable reference gene to normalize the data. It is now known that the expression of internal reference genes varies considerably during developmental stages and under different experimental conditions. For Salvia hispanica, an economically important oilseed crop, there are no reports of stable reference genes till date. In this study, we chose 13 candidate reference genes viz...
2017: PloS One
https://www.readbyqxmd.com/read/29084417/proteomics-analysis-of-medicago-truncatula-response-to-infection-by-the-phytopathogenic-bacterium-ralstonia-solanacearum-points-to-jasmonate-and-salicylate-defence-pathways
#10
Ahad Yamchi
The infection of the model legume Medicago truncatula with Ralstonia solanacearum GMI1000 gives rise to bacterial wilt disease via colonization of roots. The root and leaf responses to early infection (one and three days post infection) were characterized to investigate the molecular mechanisms of plant resistance or susceptibility. A proteomics approach based on pools of susceptible and resistant recombinant inbred lines was used to specifically target the mechanisms for tolerance. Differential abundances were evidenced for proteins involved in defense (e...
October 30, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/29081463/the-expression-of-cyclophilin-a-in-ovarian-endometrioma-its-correlation-with-recurrence-and-vascularity
#11
Akin Usta, Gulay Turan, Ertan Adali
Endometriosis is defined as the presence of functional endometrial tissues outside of the uterine cavity. Ovarian endometrioma is the most common type of endometriosis. It is an estrogen-dependent inflammatory disease that frequently causes infertility and chronic pelvic pain. Cyclophilin A (CyPA) is secreted from various types of cells in response to inflammatory stimuli. Many previous studies have shown that the increased expression and/or heightened plasma levels of CyPA exacerbates inflammation. The aim of this study is to evaluate CyPA immunoreactivity in ovarian endometrioma cyst wall...
October 2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29079163/fingolimod-induces-baff-and-expands-circulating-transitional-b-cells-without-activating-memory-b-cells-and-plasma-cells-in-multiple-sclerosis
#12
Yusei Miyazaki, Masaaki Niino, Eri Takahashi, Masako Suzuki, Masanori Mizuno, Shin Hisahara, Toshiyuki Fukazawa, Itaru Amino, Fumihito Nakano, Masakazu Nakamura, Sachiko Akimoto, Naoya Minami, Naoto Fujiki, Shizuki Doi, Shun Shimohama, Yasuo Terayama, Seiji Kikuchi
Patients with multiple sclerosis (MS) who are treated with fingolimod have an increased proportion of transitional B cells in the circulation, but the underlying mechanism is not known. We hypothesized that B cell-activating factor of the tumor necrosis factor family (BAFF) is involved in the process. Compared with healthy controls and untreated MS patients, fingolimod-treated MS patients had significantly higher serum concentrations of BAFF, which positively correlated with the proportions and the absolute numbers of transitional B cells in blood...
October 25, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29074451/mitochondrial-dysfunction-through-impaired-autophagy-leads-to-endoplasmic-reticulum-stress-deregulated-lipid-metabolism-and-pancreatitis-in-animal-models
#13
Gyorgy Biczo, Eszter T Vegh, Natalia Shalbueva, Olga A Mareninova, Jason Elperin, Ethan Lotshaw, Sophie Gretler, Aurelia Lugea, Sudarshan R Malla, David Dawson, Piotr Ruchala, Julian Whitelegge, Samuel W French, Li Wen, Sohail Z Husain, Fred S Gorelick, Peter Hegyi, Zoltan Rakonczay, Ilya Gukovsky, Anna S Gukovskaya
BACKGROUND & AIMS: Little is known about the signaling pathways that initiate and promote acute pancreatitis (AP). The pathogenesis of AP has been associated with abnormal increases in cytosolic Ca(2+), mitochondrial dysfunction, impaired autophagy, and endoplasmic reticulum (ER) stress. We analyzed the mechanisms of these dysfunctions and their relationships, and how these contribute to development of AP in mice and rats. METHODS: Pancreatitis was induced in C57BL/6J mice (control) and mice deficient in peptidylprolyl isomerase D (cyclophilin D, encoded by Ppid) by administration of L-arginine (also in rats), caerulein, bile acid, or an AP-inducing diet...
October 23, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29073139/composition-of-the-schistosoma-mansoni-worm-secretome-identification-of-immune-modulatory-cyclophilin-a
#14
Achilleas Floudas, Christopher D Cluxton, Julia Fahel, Adnan R Khan, Sean P Saunders, Sylvie Amu, Antonio Alcami, Padraic G Fallon
The helminth Schistosoma mansoni modulates the infected host's immune system to facilitate its own survival, by producing excretory/secretory molecules that interact with a variety of the host's cell types including those of the immune system. Herein, we characterise the S. mansoni adult male worm secretome and identify 111 proteins, including 7 vaccine candidates and several molecules with potential immunomodulatory activity. Amongst the molecules present in the secretome, a 17-19kDa protein analogous to human cyclophilin A was identified...
October 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29057571/cypa-and-emmprin-play-a-role-in-peri-implantitis
#15
Lei Yang, Qing Zhu, Jingjue Gong, Ming Xie, Ting Jiao
BACKGROUND: Cyclophilin A (CyPA) and Emmprin play important roles in peri-implantitis. However, their roles in the diagnosis and treatment of the disease are still unclear. PURPOSE: The aim of this split-mouth animal study was to describe variable trends of biomarkers during the progression of peri-implantitis and to define relationships among CyPA, Emmprin, and peri-implantitis. MATERIALS AND METHODS: Six male adult Labradors were used. The mandibular premolars and the first molar were extracted, and sixteen implants were placed after 3 months...
October 22, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/29054923/activity-of-the-calcineurin-pathway-in-patients-on-the-liver-transplantation-waiting-list-factors-of-variability-and-response-to-tacrolimus-inhibition
#16
Ofelia Noceti, Lucie Pouché, Patricia Esperón, Daniela Lens, Marcelo Vital, Cristina Touriño, Solange Gerona, Jean Baptiste Woillard, Pierre Marquet
BACKGROUND: We sought to evaluate, in patients on a liver transplantation waiting list, potential biomarkers of the base calcineurin pathway activity with use of a new model of nonstimulated peripheral blood mononuclear cells (PBMC) and ex vivo response to tacrolimus (TAC). METHODS: The calcineurin pathway activity was explored ex vivo in stimulated and nonstimulated PBMC from 19 patients. The inhibition of NFATC1(+) translocation to PBMC, expression of intracellular IL-2, and membrane CD25 in different T-cell subsets were measured by multiparametric flow cytometry before and after exposure to TAC...
October 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/29039975/maternal-and-cord-blood-cyclophilin-a-in-severe-preeclampsia-and-normal-pregnancy-and-its-correlation-with-vitamin-d-and-zinc
#17
Noroyono Wibowo, Riyan Hari Kurniawan, Rima Irwinda, Natasya Prameswari
OBJECTIVE: To investigate maternal and cord blood cyclophilin A level in severe preeclampsia compared with normal pregnancy and its correlation with vitamin D and zinc level. METHODS: We conducted a cross-sectional observational study of pregnant women who gave birth in Cipto Mangunkusumo Hospital between January and April 2014. RESULTS: Thirty-nine subjects were included. Subjects with severe preeclampsia have higher serum cyclophilin A levels compared with normotensive pregnancy (1299...
November 2017: Hypertension in Pregnancy
https://www.readbyqxmd.com/read/29029404/quercetin-induced-ros-production-triggers-mitochondrial-cell-death-of-human-embryonic-stem-cells
#18
So-Yeon Kim, Ho-Chang Jeong, Soon-Ki Hong, Mi-Ok Lee, Seung-Ju Cho, Hyuk-Jin Cha
Small molecules to selectively induce cell death of undifferentiated human pluripotent stem cells (hPSCs) have been developed with the aim of lowering the risk of teratoma formation during hPSC-based cell therapy. In this context, we have reported that Quercetin (QC) induces cell death selectively in hESCs via p53 mitochondrial localization. However, the detailed molecular mechanism by which hESCs undergo selective cell death induced by QC remains unclear. Herein, we demonstrate that mitochondrial reactive oxygen species (ROS), strongly induced by QC in human embryonic stem cells (hESCs) but not in human dermal fibroblasts (hDFs), were responsible for QC-mediated hESC's cell death...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29027312/cyclophilin-a-expression-and-its-prognostic-significance-in-lung-adenocarcinoma
#19
Noriyuki Nakano, Shingo Sakashita, Ryota Matsuoka, Yoshihiko Murata, Aya Shiba-Ishii, Naohiro Kobayashi, Yukio Sato, Masayuki Noguchi
Cyclophilin A (CypA) has been reported to be upregulated in malignant tumors. CypA expression is thought to be associated with acquisition of tumor growth and anti-apoptotic function. Although upregulation of CypA has been reported in lung adenocarcinoma, its clinicopathological significance and roles in malignant progression remain unclear. Here we investigated the implications of CypA expression for outcome in patients with lung adenocarcinoma. Lung adenocarcinoma specimens from 198 cases were selected and reclassified according to the World Health Organization classification (4th edition) and the Noguchi classification...
October 12, 2017: Pathology International
https://www.readbyqxmd.com/read/29019670/design-synthesis-and-biological-activities-of-vibsanin-b-derivatives-a-new-class-of-hsp90-c-terminal-inhibitors
#20
Li-Dong Shao, Jia Su, Baixin Ye, Jiang-Xin Liu, Zhi-Li Zuo, Yan Li, Yue-Ying Wang, Chengfeng Xia, Qin-Shi Zhao
Previously, vibsanin B (ViB) was found to preferentially target HSP90β compared to HSP90α. In this study, multiple experiments, including pull-down assays of biotin-ViB with recombinant HSP90β-NTD, MD, CTD, and full-length HSP90β, molecular docking of ViB and its derivatives to the HSP90 CTD, and a inhibition assay of interaction of the HSP90β CTD with GST-tagged cyclophilin 40 (Cyp40) by ViB derivatives, suggest that ViB can directly bind to the HSP90 C-terminus. On the basis of the docking predictions and primary structure-activity relationships (SARs), a series of ViB analogues devised with focus on the C18 position, along with compounds derivatized at the C4, C7, and C8 positions, were designed and chemically synthesized...
October 24, 2017: Journal of Medicinal Chemistry
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