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https://www.readbyqxmd.com/read/28534049/bio-inspired-metal-coordinate-hydrogels-with-programmable-viscoelastic-material-functions-controlled-by-longwave-uv-light
#1
Scott C Grindy, Niels Holten-Andersen
Control over the viscoelastic mechanical properties of hydrogels intended for use as biomedical materials has long been a goal of soft matter scientists. Recent research has shown that materials made from polymers with reversibly associating transient crosslinks are a promising strategy for controlling viscoelasticity in hydrogels, for example leading to systems with precisely tunable mechanical energy-dissipation. We and others have shown that bio-inspired histidine:transition metal ion complexes allow highly precise and tunable control over the viscoelastic properties of transient network hydrogels...
May 23, 2017: Soft Matter
https://www.readbyqxmd.com/read/28532149/proteomic-analysis-of-pigeonpea-cajanus-cajan-seeds-reveals-the-accumulation-of-numerous-stress-related-proteins
#2
Hari B Krishnan, Savithry S Natarajan, Nathan W Oehrle, Wesley M Garrett, Omar Darwish
Pigeonpea is one of the major sources of dietary protein for more than a billion people living in South Asia. This hardy legume is often grown in low-input and risk-prone marginal environments. Considerable research effort has been devoted by a global research consortium to develop genomic resources for the improvement of this legume crop. These efforts have resulted in the elucidation of the complete genome sequence of pigeonpea. Despite these developments, little is known about seed proteome of this important crop...
May 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28526259/the-impact-of-daytime-light-exposures-on-sleep-and-mood-in-office-workers
#3
Mariana G Figueiro, Bryan Steverson, Judith Heerwagen, Kevin Kampschroer, Claudia M Hunter, Kassandra Gonzales, Barbara Plitnick, Mark S Rea
BACKGROUND: By affecting the internal timing mechanisms of the brain, light regulates human physiology and behavior, perhaps most notably the sleep-wake cycle. Humans spend over 90% of their waking hours indoors, yet light in the built environment is not designed to affect circadian rhythms. OBJECTIVE: Using a device calibrated to measure light that is effective for the circadian system (circadian-effective light), collect personal light exposures in office workers and relate them to their sleep and mood...
June 2017: Sleep Health
https://www.readbyqxmd.com/read/28524871/in-situ-light-responses-of-the-proteorhodopsin-bearing-antarctic-sea-ice-bacterium-psychroflexus-torques
#4
David J Burr, Andrew Martin, Elizabeth W Maas, Ken G Ryan
Proteorhodopsin (PR) is a wide-spread protein found in many marine prokaryotes. PR allows for the potential conversion of solar energy to ATP, possibly assisting in cellular growth and survival during periods of high environmental stress. PR utilises either blue or green light through a single amino acid substitution. We incubated the PR-bearing bacterium Psychroflexus torquis 50 cm deep within Antarctic sea ice for 13 days, exposing cultures to diurnal fluctuations in light and temperature. Enhanced growth occurred most prominently in cultures incubated under irradiance levels of ∼50 μmol photons m(-2) s(-1), suggesting PR provides a strong selective advantage...
May 19, 2017: ISME Journal
https://www.readbyqxmd.com/read/28518150/cycloheximide-promotes-paraptosis-induced-by-inhibition-of-cyclophilins-in-glioblastoma-multiforme
#5
Lin Wang, Justin H Gundelach, Richard J Bram
Cancer is the second leading cause of death worldwide. Current treatment strategies based on multi-agent chemotherapy and/or radiation regimens have improved overall survival in some cases. However, resistance to apoptosis often develops in cancer cells, and its occurrence is thought to contribute to treatment failure. Non-apoptotic cell death mechanisms have become of great interest, therefore, in hopes that they would bypass tumor cell resistance. Glioblastoma multiforme (GBM), a grade IV astrocytic tumor is the most frequent brain tumor in adults, and has a high rate of mortality...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28515734/plant-growth-under-natural-light-conditions-provides-highly-flexible-short-term-acclimation-properties-toward-high-light-stress
#6
Tobias Schumann, Suman Paul, Michael Melzer, Peter Dörmann, Peter Jahns
Efficient acclimation to different growth light intensities is essential for plant fitness. So far, most studies on light acclimation have been conducted with plants grown under different constant light regimes, but more recent work indicated that acclimation to fluctuating light or field conditions may result in different physiological properties of plants. Thale cress (Arabidopsis thaliana) was grown under three different constant light intensities (LL: 25 μmol photons m(-2) s(-1); NL: 100 μmol photons m(-2) s(-)(1); HL: 500 μmol photons m(-2) s(-1)) and under natural fluctuating light (NatL) conditions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28510089/porphyrin-based-cationic-amphiphilic-photosensitisers-as-potential-anticancer-antimicrobial-and-immunosuppressive-agents
#7
REVIEW
Nela Malatesti, Ivana Munitic, Igor Jurak
Photodynamic therapy (PDT) combines a photosensitiser, light and molecular oxygen to induce oxidative stress that can be used to kill pathogens, cancer cells and other highly proliferative cells. There is a growing number of clinically approved photosensitisers and applications of PDT, whose main advantages include the possibility of selective targeting, localised action and stimulation of the immune responses. Further improvements and broader use of PDT could be accomplished by designing new photosensitisers with increased selectivity and bioavailability...
April 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28505496/inactivation-of-gram-bacteria-salmonella-enterica-by-chlorophyllin-based-photosensitization-mechanism-of-action-and-new-strategies-to-enhance-the-inactivation-efficiency
#8
Irina Buchovec, Viktorija Lukseviciūtė, Rita Kokstaite, Danute Labeikyte, Laura Kaziukonyte, Zivile Luksiene
This study is focused on the enhancement of susceptibility of Gram (-) bacteria S. enterica to chlorophyllin-based (Chl) photosensitization combining it with other antimicrobial tools. In order to find best combinations, the mechanism by which Chl-based photosensitization inactivates bacteria must be identified. Data confirmed that photosensitization (Chl 1.5×10(-5)M, for 1-120min, 405nm, 0-46.1J/cm(2)) reduced S. enterica population, just by 2.05 log (CFU/ml). Fluorimetric measurements indicated that just minor part of Chl was bound to Salmonella in suspension...
May 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28504210/arachidonic-acid-is-important-for-efficient-use-of-light-by-the-microalga-lobosphaera-incisa-under-chilling-stress
#9
Boris Zorin, Dipasmita Pal-Nath, Alexander Lukyanov, Sviatlana Smolskaya, Sofiya Kolusheva, Shoshana Didi-Cohen, Sammy Boussiba, Zvi Cohen, Inna Khozin-Goldberg, Alexei Solovchenko
The oleaginous microalga Lobosphaera incisa (Trebouxiophyceae, Chlorophyta) contains arachidonic acid (ARA, 20:4 n-6) in all membrane glycerolipids and in the storage lipid triacylglycerol. The optimal growth temperature of the wild-type (WT) strain is 25°C; chilling temperatures (≤15°C) slow its growth. This effect is more pronounced in the delta-5-desaturase ARA-deficient mutant P127, in which ARA is replaced with dihomo-γ-linolenic acid (DGLA, 20:3 n-6). In nutrient-replete cells grown at 25°C, the major chloroplast lipid monogalactosylglycerol (MGDG) were dominated by C18/C16 species in both strains...
May 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28503569/postneonatal-mortality-and-liver-changes-in-cloned-pigs-associated-with-human-tumor-necrosis-factor-receptor-i-fc-and-human-heme-oxygenase-1-overexpression
#10
Geon A Kim, Jun-Xue Jin, Sanghoon Lee, Anukul Taweechaipaisankul, Hyun Ju Oh, Joing-Ik Hwang, Curie Ahn, Islam M Saadeldin, Byeong Chun Lee
Soluble human tumor necrosis factor (shTNFRI-Fc) and human heme oxygenase 1 (hHO-1) are key regulators for protection against oxidative and inflammatory injury for xenotransplantation. Somatic cells with more than 10 copy numbers of shTNFRI-Fc and hHO-1 were employed in somatic cell nuclear transfer to generate cloned pigs, thereby resulting in seven cloned piglets. However, produced piglets were all dead within 24 hours after birth. Obviously, postnatal death with liver apoptosis was reported in the higher copy number of shTNFRI-Fc and hHO-1 piglets...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28503370/cave-features-seasonality-and-subterranean-distribution-of-non-obligate-cave-dwellers
#11
Enrico Lunghi, Raoul Manenti, Gentile Francesco Ficetola
Seasonality impacts species distributions through changes of the environmental conditions that affect the presence of individuals at a given place. Although the dynamics of cave microclimates are well known, only a few studies have evaluated the effects of such dynamics on non-strictly cave species. Here we assessed if species exploiting subterranean environments show changes in habitat occupation related to seasonal variation of cave microclimates. We surveyed 16 caves in central Italy every month for one year...
2017: PeerJ
https://www.readbyqxmd.com/read/28500267/impaired-mitochondrial-transcription-termination-disrupts-the-stromal-redox-poise-in-chlamydomonas
#12
Andreas Uhmeyer, Matteo Ballottari, Michela Cecchin, Lutz Wobbe
In photosynthetic eukaryotes, the metabolite exchange between chloroplast and mitochondria ensures efficient photosynthesis under saturating light conditions. The C. reinhardtii mutant stm6 is devoid of the mitochondrial transcription termination factor (mTERF) MOC1 and aberrantly expresses the mitochondrial genome, resulting in enhanced photosynthetic hydrogen production and diminished light tolerance. We analyzed the modulation of mitochondrial and chlororespiration during the acclimation of stm6 and the MOC1-complemented strain to excess light...
May 12, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28500030/translating-divergent-environmental-stresses-into-a-common-proteome-response-through-hik33-in-a-model-cyanobacterium
#13
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, Yingchun Wang
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly similar to that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival...
May 12, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28499880/photoprotection-strategies-of-the-alga-nannochloropsis-gaditana
#14
Volha U Chukhutsina, Rikard Fristedt, Tomas Morosinotto, Roberta Croce
Nannochloropsis spp. are algae with high potential for biotechnological applications due to their capacity to accumulate lipids. However, little is known about their photosynthetic apparatus and acclimation/photoprotective strategies. In this work, we studied the mechanisms of non-photochemical quenching (NPQ), the fast response to high light stress, in Nannochloropsis gaditana by "locking" the cells in six different states during quenching activation and relaxation. Combining biochemical analysis with time-resolved fluorescence spectroscopy, we correlated each NPQ state with the presence of two well-known NPQ components: de-epoxidized xanthophylls and stress-related antenna proteins (LHCXs)...
May 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28496465/effect-of-nonionic-surfactants-dodecyl-maltoside-and-polysorbate-20-on-prevention-of-aggregation-and-conformational-changes-of-recombinant-human-ifn%C3%AE-_1b-induced-by-light
#15
Najmeh Mahjoubi, Ahmad Fazeli, Rassoul Dinarvand, Mohammad Reza Khoshayand, Maryam Shekarchi, Mohammad Reza Fazeli
Liquid protein formulations are prone to form aggregates. The effect of nonionic surfactants such as Polysorbate 20 (PS 20) and n-Dodecyl β-D-maltoside (DDM) on the prevention of aggregation and conformational changes of recombinant human IFNβ-1b (rhIFN β_1b) was explored. Polysorbate has been used in formulations of protein pharmaceuticals. There have been concerns about using PS 20 due to its residual peroxide content which may negatively affect protein efficacy. n-Dodecyl β-D-maltoside has been of interest and shown to be highly effective in prevention of aggregation...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28492186/recent-research-trends-in-dentistry
#16
REVIEW
Shobha Tandon, Annapurny Venkiteswaran, Sudhindra M Baliga, Ullal Anand Nayak
Research being an investigative process is employed to increase or revise the current knowledge. Scientific research involves the conduct of a methodical study to prove a hypothesis or give an answer to a specific question with the main aim of finding definitive answer. This paper aims to advance knowledge of research and develop interest in the postgraduate students. It also throws light on the existing and emerging research strengths within a "high-performance culture." The trends in dental research worldwide are looked at, in particular, a comparison between the publication status in two countries, namely India and Australia...
April 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28489898/comparative-genomic-study-of-aldh-gene-superfamily-in-gossypium-a-focus-on-gossypium-hirsutum-under-salt-stress
#17
Yating Dong, Hui Liu, Yi Zhang, Jiahui Hu, Jiyu Feng, Cong Li, Cheng Li, Jinhong Chen, Shuijin Zhu
Aldehyde dehydrogenases (ALDHs) are a superfamily of enzymes which play important role in the scavenging of active aldehydes molecules. In present work, a comprehensive whole-genomic study of ALDH gene superfamily was carried out for an allotetraploid cultivated cotton species, G. hirsutum, as well as in parallel relative to their diploid progenitors, G. arboreum and G. raimondii. Totally, 30 and 58 ALDH gene sequences belong to 10 families were identified from diploid and allotetraploid cotton species, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28488438/mechanically-robust-stretchable-solar-absorbers-with-submicron-thick-multilayer-sheets-for-wearable-and-energy-applications
#18
Hye Jin Lee, Dae-Han Jung, Tae-Hyeon Kil, Sang Hyeon Kim, Ki-Suk Lee, Seung-Hyub Baek, Won Jun Choi, Jeong Min Baik
A facile method to fabricate a mechanically robust, stretchable solar absorber for stretchable heat generation and an enhanced thermoelectric generator (TEG) is demonstrated. This strategy is very simple: it uses a multilayer film made of titanium and magnesium fluoride optimized by a two-dimensional finite element frequency-domain simulation, followed by the application of mechanical stresses such as bending and stretching to the film. This process produces many microsized sheets with submicron thickness (∼500 nm), showing great adhesion to any substrates such as fabrics and polydimethylsiloxane...
May 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28488174/characterization-of-apolipoprotein-c3-apo-c3-lna-dna-impurities-and-degradation-products-by-lc-ms-ms
#19
Olga V Friese, Justin B Sperry, Yan He, Liji Joseph, James A Carroll, Jason C Rouse
Apolipoprotein C3 (Apo C3) LNA/DNA gapmer was evaluated under various stress and formulation conditions for the purpose of its development as a potential biotherapeutic for low density lipoprotein (LDL) lowering. Using ion-pairing (IP) reversed-phase (RP) liquid chromatography ultra-high resolution (UHR) tandem mass spectrometry (IP-RPLC-MS/MS), a combination of accurate mass measurements and collision-induced dissociation enabled in-depth characterization of Apo C3 LNA/DNA oligonucleotide, in particular the inherent impurities following synthesis and degradation products after exposure to stress conditions...
May 9, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28486137/cloning-and-characterization-of-a-novel-gigantea-gene-in-sweet-potato
#20
Wei Tang, Hui Yan, Zai-Xing Su, Sung-Chul Park, Ya-Ju Liu, Yun-Gang Zhang, Xin Wang, Meng Kou, Dai-Fu Ma, Sang-Soo Kwak, Qiang Li
The transition from vegetative to reproductive growth, a key event in the lifecycle of a plant, is affected by environmental stresses. The flowering-time regulator GIGANTEA (GI) may be contributing to susceptibility of the regulation of photoperiodic flowering, circadian rhythm control, and abiotic stress resistance in Arabidopsis. However, the role of GI in sweet potato remains unknown. Here, we isolated and characterized a GI gene (IbGI) from sweet potato (Ipomoea batatas [L.] Lam). The IbGI cDNA sequence was isolated based on information from a sweet potato transcriptome database...
April 27, 2017: Plant Physiology and Biochemistry: PPB
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