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Pancreatitis AND microRNA

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https://www.readbyqxmd.com/read/28813430/reprogramming-human-gallbladder-cells-into-insulin-producing-%C3%AE-like-cells
#1
Feorillo Galivo, Eric Benedetti, Yuhan Wang, Carl Pelz, Jonathan Schug, Klaus H Kaestner, Markus Grompe
The gallbladder and cystic duct (GBCs) are parts of the extrahepatic biliary tree and share a common developmental origin with the ventral pancreas. Here, we report on the very first genetic reprogramming of patient-derived human GBCs to β-like cells for potential autologous cell replacement therapy for type 1 diabetes. We developed a robust method for large-scale expansion of human GBCs ex vivo. GBCs were reprogrammed into insulin-producing pancreatic β-like cells by a combined adenoviral-mediated expression of hallmark pancreatic endocrine transcription factors PDX1, MAFA, NEUROG3, and PAX6 and differentiation culture in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28782557/yb-1-expression-promotes-pancreatic-cancer-metastasis-that-is-inhibited-by-microrna-216a
#2
Jingjing Lu, Xiaohong Li, Fei Wang, Yibing Guo, Yan Huang, Hui Zhu, Yao Wang, Yuhua Lu, Zhiwei Wang
Pancreatic cancer is one of the most aggressive cancers. The vast majority of patients are diagnosed with advanced, unresectable disease because of early invasive growth and metastatic spread. The aim of this study was to examine YB-1 expression in pancreatic cancer and determine its effects on cell invasion. YB-1 is overexpressed in pancreatic cancer cell lines and patient tissue samples. In patient tissues, high YB-1 levels correlated with perineural invasion. Silencing of YB-1 significantly reduced cell invasion with decreased expression of MMPs in vitro...
August 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28761107/regulatory-t-cells-from-pancreatic-lymphnodes-of-patients-with-type-1-diabetes-express-increased-levels-of-microrna-mir-125a-5p-that-limits-ccr2-expression
#3
Guido Sebastiani, Giuliana Ventriglia, Angela Stabilini, Carlo Socci, Cristina Morsiani, Andrea Laurenzi, Laura Nigi, Caterina Formichi, Bechara Mfarrej, Alessandra Petrelli, Georgia Fousteri, Todd M Brusko, Francesco Dotta, Manuela Battaglia
Autoimmune type 1 diabetes (T1D) is thought to be caused by a defective immune regulation with regulatory T (Treg) cells playing a fundamental role in this process. Tolerance mechanisms depend on tunable responses that are sensitive to minor perturbations in the expression of molecules that can be carried out by multiple epigenetic mechanisms, including regulation by microRNAs. In this study, microRNA expression profile was investigated in Treg cells isolated from peripheral blood (PB) and from pancreatic draining lymph nodes (PLN) of T1D patients and non-diabetic subjects...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759959/low-serum-mir-373-predicts-poor-prognosis-in-patients-with-pancreatic-cancer
#4
Yongqiang Hua, Hao Chen, Libing Wang, Feng Wang, Peng Wang, Zhouyu Ning, Ye Li, Luming Liu, Zhen Chen, Zhiqiang Meng
BACKGROUND: Circulating microRNAs (miRNAs) are emerging as novel biomarkers for various types of cancer including pancreatic cancer (PC). OBJECTIVE: We aimed to explore the diagnostic and prognostic significance of serum miR-373 in PC. METHODS: In the current study, we recruited a total of 103 PC patients, 30 patients with benign pancreatic tumor, 20 patients with chronic pancreatitis and 50 healthy volunteers. Total RNA was isolated from all the blood samples, and relative miR-373 expression levels were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR)...
July 19, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28753564/exportin-1-xpo1-inhibition-leads-to-restoration-of-tumor-suppressor-mir-145-and-consequent-suppression-of-pancreatic-cancer-cell-proliferation-and-migration
#5
Asfar S Azmi, Yiwei Li, Irfana Muqbil, Amro Aboukameel, William Senapedis, Erkan Baloglu, Yosef Landesman, Sharon Shacham, Michael G Kauffman, Philip A Philip, Ramzi M Mohammad
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer related deaths in the United States with a majority of these patients dying from aggressively invasive and metastatic disease. There is growing evidence that suggests an important role for microRNAs (miRNAs) in the pathobiology of aggressive PDAC. In this study, we found that the expression of miR-145 was significantly lower in PDAC cells when compared to normal pancreatic duct epithelial cells. Here we show that inhibition of the nuclear exporter protein exportin 1 (XPO1; also known as chromosome maintenance region 1 [CRM1]) by siRNA knockdown or by the Selective Inhibitor of Nuclear Export (SINE) compound (KPT-330; selinexor) increases miR-145 expression in PDAC cells resulting in the decreased cell proliferation and migration capacities...
July 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28749040/retraction-microrna-4656-is-a-prognostic-factor-and-tumor-suppressor-in-human-pancreatic-cancer-through-a-downstream-target-of-trka-by-xianglong-tan-jinyong-lv-guodong-zhao-zhiming-zhao-chenggang-li-yong-xu-and-minggen-hu
#6
https://www.readbyqxmd.com/read/28747214/culturing-and-transcriptome-profiling-of-progenitor-like-colonies-derived-from-adult-mouse-pancreas
#7
Dongshen Ma, Shanshan Tang, Jing Song, Qiong Wu, Fangfang Zhang, Yun Xing, Yi Pan, Yanfeng Zhang, Jingwei Jiang, Yubin Zhang, Liang Jin
BACKGROUND: Transplantation of insulin-producing cells is considered an important diabetes therapy. Many research studies have shown that insulin-producing cells can be derived from the in-vitro cultured pancreatic colonies with self-renewal ability and multilineage potential. Even though these progenitor-like colonies have been prepared from adult pancreas cells, the efficient culture method is hardly established and regulation of the colonies is rarely known. We confirmed previously that single cells acquired from adult mouse pancreas could form cyst-like colonies in a 3D semi-solid system containing Matrigel and methylcellulose...
July 26, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28721656/circrna-a-novel-type-of-biomarker-for-cancer
#8
REVIEW
He-da Zhang, Lin-Hong Jiang, Da-Wei Sun, Jun-Chen Hou, Zhen-Ling Ji
Circular RNAs (circRNAs) are a class of long, non-coding RNAs molecules that shape a covalently closed continuous loop which have no 5'-3' polarity and contain no polyA tail. CircRNAs also possess relatively jarless framework and are highly tissue-specific expressed in the eukaryotic transcriptome. Emerging evidences have discovered that thousands of endogenous circRNAs are present in mammalian cells and they mediate gene expression at the transcriptional or post-transcriptional level by binding to microRNAs or other molecules and then inhibit their function...
July 18, 2017: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/28721157/micrornas-and-metabolic-disorders-where-are-we-heading
#9
Agnieszka Sliwinska, Marta A Kasinska, Jozef Drzewoski
MicroRNAs (miRNAs, miRs) are short, non-coding molecules engaged in normal functioning of eukaryotic cells, as negative regulators of gene expression. Since the first discovery of miRNA in the early 1990s, hundreds of different miRNAs and their targets have been identified. A growing number of studies have aimed to search for microRNAs which have a key role in the regulation of insulin signaling and metabolic homeostasis. Recent evidence indicates that dysregulation of miRNA expression is involved in the development of various diseases, including type 2 diabetes mellitus (T2DM), obesity and cardiovascular diseases...
June 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28720759/depleted-tumor-suppressor-mir-107-in-plasma-relates-to-tumor-progression-and-is-a-novel-therapeutic-target-in-pancreatic-cancer
#10
Taisuke Imamura, Shuhei Komatsu, Daisuke Ichikawa, Mahito Miyamae, Wataru Okajima, Takuma Ohashi, Jun Kiuchi, Keiji Nishibeppu, Hirotaka Konishi, Atsushi Shiozaki, Ryo Morimura, Hisashi Ikoma, Toshiya Ochiai, Kazuma Okamoto, Hiroki Taniguchi, Eigo Otsuji
This study explored decreased tumor suppressor microRNA (miRNA) plasma levels in pancreatic cancer (PCa) patients to clarify their potential as novel biomarkers and therapeutic targets. We used the microRNA array-based approach to select candidates by comparing plasma levels between PCa patients and healthy volunteers. Six down-regulated miRNAs (miR-107, miR-126, miR-451, miR-145, miR-491-5p, and miR-146b-5p) were selected. Small- and large-scale analyses using samples from 100 PCa patients and 80 healthy volunteers revealed that miR-107 was the most down-regulated miRNA in PCa patients compared with healthy volunteers (P < 0...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28713945/mir%C3%A2-433-protects-pancreatic-%C3%AE-cell-growth-in-high%C3%A2-glucose-conditions
#11
Min Wang
Pancreatic β cell dysfunction is a key characteristic in the pathogenesis of diabetes mellitus (DM). MicroRNAs (miRNAs) have been identified to serve a role in DM pathogenesis, but how specific miRNAs regulate glucose‑stimulated β cell functions remain unclear. The present study aimed to explore the effects of miR‑433 on cell growth under high‑glucose culture conditions and to determine the possible mechanisms involved. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis was performed to detect the expression levels of miRNAs in Min‑6 pancreatic β cells cultured in high‑glucose medium, which revealed that miR‑433 was significantly downregulated...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28710412/exosomes-derived-from-pancreatic-cancer-cells-induce-insulin-resistance-in-c2c12-myotube-cells-through-the-pi3k-akt-foxo1-pathway
#12
Lantian Wang, Bo Zhang, Wen Zheng, Muxing Kang, Qing Chen, Wenjie Qin, Chao Li, Yuefeng Zhang, Yingkuan Shao, Yulian Wu
Prospective epidemiological studies have consistently suggested that pancreatic cancer-associated new-onset diabetes mellitus (PC-DM) represents a potential platform for early diagnose of pancreatic cancer (PC). Despite the studies performed, the mechanism behind this phenomenon remains ambiguous. In this study, we explored the effects of two types of exosomes released by murine pancreatic cancer and ductal epithelial cells on murine skeletal muscle cells. The results show that PC-derived exosomes can readily enter C2C12 myotubes, triggering lipidosis and glucose intake inhibition...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28693279/microrna-215-acts-as-a-tumor-suppressor-in-breast-cancer-by-targeting-akt-serine-threonine-kinase-1
#13
Jian Yao, Ping Zhang, Jin Li, Wei Xu
There are accumulating reports that microRNAs are dysregulated in a number of human cancer types, and that they may function as tumor suppressors or oncogenes in tumorigenesis and tumor development. microRNA-215 (miR-215) has been identified as a tumor suppressor in epithelial ovarian, pancreatic, non-small cell lung and colon cancer, whereas it may act as an oncogene in gastric and cervical cancer. The role of miR-215 in breast cancer carcinogenesis and progression has yet to be elucidated. In the present study, the expression level of miR-215 was determined in breast cancer tissues and cell lines using the reverse transcription-quantitative polymerase chain reaction...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28688106/human-milk-exosomes-and-their-micrornas-survive-digestion-in-vitro-and-are-taken-up-by-human-intestinal-cells
#14
Yalin Liao, Xiaogu Du, Jie Li, Bo Lönnerdal
SCOPE: Human milk exosomes provide a natural means of genetic material transfer to infants; however, the effect of gastric/pancreatic digestion milk exosomes stability and their microRNA content is largely unknown. METHODS AND RESULTS: We took a simulated gastric/pancreatic digestion protocol to perform in vitro digestion of milk exosomes, explore intestinal epithelial uptake, and further elucidate microRNA responses to digestion at early-, mid-, -late lactation by massive parallel sequencing...
July 7, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28686518/connexins-and-micrornas-interlinked-players-in-regulating-islet-function
#15
Malati R Umrani, Mugdha V Joglekar, Ella Somerville Glover, Wilson Wong, Anandwardhan A Hardikar
Pancreatic β-cells are connected to neighboring endocrine cells through the adherin proteins and gap junctions. Connexin 36 (Cx36) is one of the most well-studied and abundantly expressed gap-junction proteins within rodent islets, which is important in coordinated insulin secretion. The expression of connexins is regulated at various levels and by several mechanisms; one of which is via microRNAs. In past 2 decades, microRNAs (miRNAs) have emerged as key molecules in developmental, physiologic and pathological processes...
July 7, 2017: Islets
https://www.readbyqxmd.com/read/28685847/microrna-300-promotes-apoptosis-and-inhibits-proliferation-migration-invasion-and-epithelial-mesenchymal-transition-via-the-wnt-%C3%AE-catenin-signaling-pathway-by-targeting-cul4b-in-pancreatic-cancer-cells
#16
Jia-Qiang Zhang, Shi Chen, Jiang-Ning Gu, Yi Zhu, Qian Zhan, Dong-Feng Cheng, Hao Chen, Xia-Xing Deng, Bai-Yong Shen, Cheng-Hong Peng
The study aims to verify the hypothesis that up-regulation of microRNA-300 (miR-300) targeting CUL4B promotes apoptosis and suppresses proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of pancreatic cancer cells by regulating the Wnt/β-catenin signaling pathway. Pancreatic cancer tissues and adjacent tissues were collected from 110 pancreatic cancer patients. Expression of miR-300, CUL4B, Wnt, β-catenin, E-cadherin, N-cadherin, Snail, GSK-3β, and CyclinD1 were detected using qRT-PCR and Western blot...
July 7, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28685672/micrornas-mediated-mmp-regulation-current-diagnostic-and-therapeutic-strategies-for-metabolic-syndrome
#17
Sharad Saxena, Aditi Jain, Vibha Rani
Metabolic syndrome (MS) is a global socioeconomic problem rapidly progressing in accordance with increasing body mass index (BMI) and age. It is a consortium of risk factors, such as dyslipidaemia, insulin resistance, leptin resistance, reduced adiponectin, glucose intolerance, hyperglycemia, and hypertension. Collectively, these factors accelerate the onset of type 2 diabetes mellitus, cardiovascular disease, stroke, and certain cancers such as breast, liver pancreatic, and colon cancer. Extracellular matrix (ECM) and basement membrane remodeling play a central role during pathogenesis of MS as they regulate diverse cell functions including proliferation, differentiation, and migration...
July 7, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28677798/microrna-448-suppresses-metastasis-of-pancreatic-ductal-adenocarcinoma-through-targeting-jak1-stat3-pathway
#18
Dan-Li Yu, Tao Zhang, Kun Wu, Yan Li, Juan Wang, Jun Chen, Xiao-Quan Li, Xing-Guo Peng, Jia-Ning Wang, Li-Guo Tan
Pancreatic ductal adenocarcinoma (PDAC) is the most common type of malignant pancreatic tumor. MicroRNAs (miRNAs) are a group of small, non-protein coding, endogenous RNAs that play critical roles in tumorigenesis and progression of PDAC. In the present study, we demonstrated that miR-448 expression was downregulated in PDAC tissues and cell lines. Clinical association analysis indicated that low expression of miR-448 was associated with poor prognostic features and conferred a significant reduced survival of PDAC patients...
August 2017: Oncology Reports
https://www.readbyqxmd.com/read/28677209/down-regulation-of-ulk1-by-microrna-372-inhibits-the-survival-of-human-pancreatic-adenocarcinoma-cells
#19
Hongxi Chen, Zhipeng Zhang, Yebin Lu, Kun Song, Xiwu Liu, Fada Xia, Weijia Sun
Dysregulation of microRNA (miRNA) expression in various cancers and their role in cancer progression is well documented. The purpose of this study was to investigate the biological role of miR-372 in human pancreatic adenocarcinoma (HPAC). We collected 20 pairs of HPAC tissues and adjacent non-cancerous tissues to detect miR-372 expression levels. We transfected BXPC-3 and PANC-1 cells with miR-372 inhibitor/ mimics to study their effect on cell proliferation, apoptosis, invasion, migration and autophagy. In addition, miR-372 mimics and a tumor protein UNC51-like kinase 1 (ULK1) siRNA were co-transfected into BXPC-3 and PANC-1 cells to explore the mechanism of miR-372 and ULK1 on HPAC tumorigenesis...
July 5, 2017: Cancer Science
https://www.readbyqxmd.com/read/28674733/micrornas-modulate-core-clock-gene-expression-in-pancreatic-islets-during-early-postnatal-life-in-rats
#20
Cécile Jacovetti, Adriana Rodriguez-Trejo, Claudiane Guay, Jonathan Sobel, Sonia Gattesco, Volodymyr Petrenko, Camille Saini, Charna Dibner, Romano Regazzi
AIMS/HYPOTHESIS: Evidence continues to emerge detailing a fine-tuning of the regulation of metabolic processes and energy homeostasis by cell-autonomous circadian clocks. Pancreatic beta cell functional maturation occurs after birth and implies transcriptional changes triggered by a shift in the nutritional supply that occurs at weaning, enabling the adaptation of insulin secretion. So far, the developmental timing and exact mechanisms involved in the initiation of the circadian clock in the growing pancreatic islets have never been addressed...
July 4, 2017: Diabetologia
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