Yujia Zhao, Jianlin Yuan, Dai Xiao, Luwen Zhang, Cheng Li, Jingfei Hu, Rui Chen, Daili Song, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Qigui Yan, Xinfeng Han, Xintian Wen, Sanjie Cao, Xiaobo Huang
Porcine deltacoronavirus (PDCoV) is an emerging enteropathogenic coronavirus. It causes mortality in neonatal piglets and is of growing concern because of its broad host range, including humans. To date, the mechanism of PDCoV infection remains poorly understood. Here, based on a genome-wide CRISPR screen of PDCoV-infected cells, we found that HSP90AB1 promotes PDCoV infection. Knockdown or knockout of HSP90AB1 in LLC-PK cells resulted in a significantly suppressed PDCoV infection. Infected cells treated with HSP90 inhibitors 17-AAG and VER-82576 also showed a significantly suppressed PDCoV infection, although KW-2478, which does not affect the ATPase activity of HSP90AB1, had no effect on PDCoV infection...
December 11, 2023: Journal of Biological Chemistry