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https://www.readbyqxmd.com/read/28793031/airborne-fungi-in-an-intensive-care-unit
#1
C L Gonçalves, F V Mota, G F Ferreira, J F Mendes, E C Pereira, C H Freitas, J N Vieira, J P Villarreal, P S Nascente
The presence of airborne fungi in Intensive Care Unit (ICUs) is associated with increased nosocomial infections. The aim of this study was the isolation and identification of airborne fungi presented in an ICU from the University Hospital of Pelotas - RS, with the attempt to know the place's environmental microbiota. 40 Petri plates with Sabouraud Dextrose Agar were exposed to an environment of an ICU, where samples were collected in strategic places during morning and afternoon periods for ten days. Seven fungi genera were identified: Penicillium spp...
July 27, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28771547/evaluation-of-multifarious-plant-growth-promoting-traits-antagonistic-potential-and-phylogenetic-affiliation-of-rhizobacteria-associated-with-commercial-tea-plants-grown-in-darjeeling-india
#2
Jintu Dutta, Debajit Thakur
Plant growth promoting rhizobacteria (PGPR) are studied in different agricultural crops but the interaction of PGPR of tea crop is not yet studied well. In the present study, the indigenous tea rhizobacteria were isolated from seven tea estates of Darjeeling located in West Bengal, India. A total of 150 rhizobacterial isolates were screened for antagonistic activity against six different fungal pathogens i.e. Nigrospora sphaerica (KJ767520), Pestalotiopsis theae (ITCC 6599), Curvularia eragostidis (ITCC 6429), Glomerella cingulata (MTCC 2033), Rhizoctonia Solani (MTCC 4633) and Fusarium oxysporum (MTCC 284), out of which 48 isolates were antagonist to at least one fungal pathogen used...
2017: PloS One
https://www.readbyqxmd.com/read/28761108/the-advantages-of-endophyte-infected-over-uninfected-tall-fescue-in-the-growth-and-pathogen-resistance-are-counteracted-by-elevated-co2
#3
Wei Chen, Hui Liu, Wurihan, Yubao Gao, Stuart D Card, Anzhi Ren
Atmospheric CO2 concentrations are predicted to double within the next century. Despite this trend, the extent and mechanisms through which elevated CO2 affects grass-endophyte symbionts remain uncertain. In the present study, the growth, chemical composition and pathogen resistance of endophyte-infected (E+) and uninfected (E-) tall fescue were compared under elevated CO2 conditions. The results showed that the effect of endophyte infection on the growth of tall fescue was significantly affected by elevated CO2...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28757872/production-characterization-and-antimicrobial-activity-of-mycocin-produced-by-debaryomyces-hansenii-dsmz70238
#4
Safaa A S Al-Qaysi, Halah Al-Haideri, Zaid Akram Thabit, Wijdan Hameed Abd Al-Razzaq Al-Kubaisy, Jamal Abd Al-Rahman Ibrahim
The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, and Candida neoformans, respectively...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28732400/solar-photocatalytic-disinfection-of-agricultural-pathogenic-fungi-curvularia-sp-in-real-urban-wastewater
#5
Yelitza Aguas, Margarita Hincapie, Pilar Fernández-Ibáñez, María Inmaculada Polo-López
The interest in developing alternative water disinfection methods that increase the access to irrigation water free of pathogens for agricultural purposes is increasing in the last decades. Advanced Oxidation Processes (AOPs) have been demonstrated to be very efficient for the abatement of several kind of pathogens in contaminated water. The purpose of the current study was to evaluate and compare the capability of several solar AOPs for the inactivation of resistant spores of agricultural fungi. Solar photoassisted H2O2, solar photo-Fenton at acid and near-neutral pH, and solar heterogeneous photocatalysis using TiO2, with and without H2O2, have been studied for the inactivation of spores of Curvularia sp...
July 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28722254/determination-of-irritant-threshold-concentrations-of-multiple-tree-grass-weed-and-mould-allergens-for-intradermal-testing-of-horses-residing-in-the-southern-usa
#6
Martha J Lane, Cherie M Pucheu-Haston, Michael T Kearney, Michelle Woodward
BACKGROUND: Appropriate allergen threshold concentrations (TCs) for intradermal testing (IDT) have not been established in horses for many pollen and mould allergens. OBJECTIVES: To determine the TCs in non-allergic horses and describe the frequency of late phase reactions for 26 allergens, including trees, grasses, weeds and moulds in horses residing in the southern Unites States. ANIMALS: Twenty four clinically normal horses in the southern United States...
July 18, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28663602/genera-of-phytopathogenic-fungi-gophy-1
#7
Y Marin-Felix, J Z Groenewald, L Cai, Q Chen, S Marincowitz, I Barnes, K Bensch, U Braun, E Camporesi, U Damm, Z W de Beer, A Dissanayake, J Edwards, A Giraldo, M Hernández-Restrepo, K D Hyde, R S Jayawardena, L Lombard, J Luangsa-Ard, A R McTaggart, A Y Rossman, M Sandoval-Denis, M Shen, R G Shivas, Y P Tan, E J van der Linde, M J Wingfield, A R Wood, J Q Zhang, Y Zhang, P W Crous
Genera of Phytopathogenic Fungi (GOPHY) is introduced as a new series of publications in order to provide a stable platform for the taxonomy of phytopathogenic fungi. This first paper focuses on 21 genera of phytopathogenic fungi: Bipolaris, Boeremia, Calonectria, Ceratocystis, Cladosporium, Colletotrichum, Coniella, Curvularia, Monilinia, Neofabraea, Neofusicoccum, Pilidium, Pleiochaeta, Plenodomus, Protostegia, Pseudopyricularia, Puccinia, Saccharata, Thyrostroma, Venturia and Wilsonomyces. For each genus, a morphological description and information about its pathology, distribution, hosts and disease symptoms are provided...
March 2017: Studies in Mycology
https://www.readbyqxmd.com/read/28578573/antifungal-and-antiviral-cyclic-peptides-from-the-deep-sea-derived-fungus-simplicillium-obclavatum-eiodsf-020
#8
Xiao Liang, Xu-Hua Nong, Zhong-Hui Huang, Shu-Hua Qi
A new linear peptide simplicilliumtide I (1) and four new cyclic peptides simplicilliumtides J-M (2-5) together with known analogues verlamelins A and B (6 and 7) were isolated from the deep-sea-derived fungal strain Simplicillium obclavatum EIODSF 020. Their structures were elucidated by spectroscopic analysis, and their absolute configurations were further confirmed by chemical structural modification, Marfey's and Mosher's methods. Compounds 2, 6, and 7 showed significant antifungal activity toward Aspergillus versicolor and Curvularia australiensis and also had obvious antiviral activity toward HSV-1 with IC50 values of 14...
June 28, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28573998/corneal-ulcer-due-to-a-rare-pleosporalean-member-of-the-genus-bipolaris-following-cow-tail-injury-to-the-eye-a-case-report-and-review-of-literature
#9
H Vijaya Pai, Esha Jamal, Prakash Peralam Yegneswaran
Aspergillus and Fusarium are the most common fungi causing mycotic keratitis. Injury to the eye with vegetable matter, cow tail injury, long-term use of topical steroids are some of the risk factors for mycotic keratitis. There are few case reports of keratitis caused by Bipolaris. The human pathogenic species in the genus are Bipolaris spicifera, Bipolaris hawaiiensis, Bipolaris papendorfii, and Bipolaris australiensis. Most commonly reported keratitis is caused by B. hawaiiensis, followed by B. spicifera...
May 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28548142/halofunctionalization-of-alkenes-by-vanadium-chloroperoxidase-from-curvularia-inaequalis
#10
Jia Jia Dong, Elena Fernández-Fueyo, Jingbo Li, Zheng Guo, Rokus Renirie, Ron Wever, Frank Hollmann
The vanadium-dependent chloroperoxidase from Curvularia inaequalis is a stable and efficient biocatalyst for the hydroxyhalogenation of a broad range of alkenes into halohydrins. Up to 1 200 000 TON with 69 s(-1) TOF were observed for the biocatalyst. A bienzymatic cascade to yield epoxides as reaction products is presented.
June 6, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28480570/in-vitro-antifungal-probiotic-and-antioxidant-functional-properties-of-a-novel-lactobacillus-paraplantarum-isolated-from-fermented-dates-in-saudi-arabia
#11
Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi
BACKGROUND: Fermented foods produced using dates are used in Gulf countries as beneficial and healthful foods. The beneficial microbial flora in fermented dates contributes to maintaining the nutritional properties of dates by preventing the growth of spoilage fungi. Here, we examined the antifungal, probiotic, and antioxidant properties of the novel Lactobacillus strain D-3 isolated from fermented dates. RESULTS: Analyzing the morphological, physiological, and biochemical characteristics of this strain demonstrated that it was similar to Lactobacillus species, and molecular level amplification of the 16S rRNA gene showed that it belonged to Lactobacillus paraplantarum...
May 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28417063/melanized-fungus-as-an-epidural-abscess-a-diagnostic-and-therapeutic-challenge
#12
Adesh Shrivastava, Karuna Tadepalli, Garima Goel, Kajal Gupta, Pradeep Kumar Gupta
Curvularia a dematiaceous fungus is ubiquitously found in soil around the world. We report an epidural abscess due to Curvularia lunata in a 48 years male farmer who underwent decompressive laminectomy as primary modality of treatment followed by isolation, identification and confirmation of the isolate from tissue by ITS sequencing. Antifungal therapy with voriconazole and amphotericin B for 3 and 2 weeks respectively improved patient's condition and is presently on regular follow up with no sequelae since last 7 months...
June 2017: Medical Mycology Case Reports
https://www.readbyqxmd.com/read/28393907/involvement-of-a-velvet-protein-clvelb-in-the-regulation-of-vegetative-differentiation-oxidative-stress-response-secondary-metabolism-and-virulence-in-curvularia-lunata
#13
Jin-Xin Gao, Chuan-Jin Yu, Meng Wang, Jia-Nan Sun, Ya-Qian Li, Jie Chen
The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28389086/developing-eco-friendly-biofungicide-for-the-management-of-major-seed-borne-diseases-of-rice-and-assessing-their-physical-stability-and-storage-life
#14
Ramasamy Naveenkumar, Arjunan Muthukumar, Ganesan Sangeetha, Ramanathan Mohanapriya
Three plant oils (Cymbopogon citratus, Cymbopogon martini, and Pelargonium graveolens) were developed as EC formulations and tested for their physical stabilities. EC formulations (10EC, 20EC and 30EC) of C. citratus, C. martini and P. graveolens had emulsion stability, spontaneity property, heat and cold stability. EC formulated plant oils were screened against the major seed borne fungi of rice such as Curvularia lunata, Fusarium moniliforme, Bipolaris oryzae, and Sarocladium oryzae. The level of inhibition varied among the concentrations of EC formulations...
April 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/28383602/fungal-contamination-and-disinfection-of-dental-chairs-teresina-piaui-brazil
#15
Antônio Iv de Almondes, Jhonatta Op de Araújo, Larissa Mendes de Sirqueira Amaral, Renandro C Reis, Jhonatas Cs Porto, João Batista Teles, Thiago L Monte, Iraci de Ms Noleto, Tanit C Santos, Ivonizete P Ribeiro, Mitra Mobin
The aim of this study was to analyze fungal contamination on dental chairs at the clinic of a Higher Education Institution in TeresinaPI, Brazil, and to evaluate the effectiveness of different disinfectants: 70% alcohol and 1% sodium hypochlorite. We selected the five sites with most contact between patient and chair: headrest, backrest, armrests, seat and foot rest. Samples were collected from these sites on 14 chairs and inoculated in agar Sabouraud culture medium containing chloramphenicol. Pathogenic fungi were isolated from all sampling sites on the chairs...
December 2016: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/28361537/integration-of-chemical-genetic-and-bioinformatic-approaches-delineates-fungal-polyketide-peptide-hybrid-biosynthesis
#16
Mamoru Yokoyama, Yuichiro Hirayama, Tsuyoshi Yamamoto, Shinji Kishimoto, Yuta Tsunematsu, Kenji Watanabe
To identify natural products and their associated biosynthetic genes from underutilized, difficult-to-manipulate microbes, chemical screening and bioinformatic analysis were employed to identify secondary metabolites and a potentially associated biosynthetic gene cluster. Subsequently, a heterologous expression system was used to confirm the identity of the gene cluster and the proposed biosynthetic mechanism. This approach successfully identified the curvupallide and spirostaphylotrichin biosynthetic pathways in endophytic fungus Curvularia pallescens and the short-chain pyranonigrin biosynthetic pathway in Aspergillus niger...
March 31, 2017: Organic Letters
https://www.readbyqxmd.com/read/28353091/diversity-of-culturable-fungi-inhabiting-petroleum-contaminated-soils-in-southern-iran
#17
Elham Mohammadian, Mahdi Arzanlou, Asadollah Babai-Ahari
The present study was aimed at characterising species diversity of fungi inhabiting petroleum-contaminated soils of oil fields in a southern region of Iran. Two different techniques were used for fungal isolation including enrichment on atmospheres of phenolic hydrocarbons and crude oil as substrate. Phylogenetic analysis of the internal transcribed spacer of ribosomal DNA was used for taxonomic identification with additional information from the β-tubulin gene for selected taxa. Overall, 183 strains from 14 genera and five orders were obtained: Pleosporales (Alternaria, Curvularia, Stemphylium, Ulocladium), Chaetothyriales (Exophiala), Eurotiales (Aspergillus), Hypocreales (Acremonium, Emericellopsis, Sarocladium, Stachybotrys, Fusarium, Trichoderma, Beauveria), and Capnodiales (Cladosporium)...
March 28, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28306602/cure-rate-of-fungal-keratitis-with-antibacterial-therapy
#18
Alice Y Matoba, Ryan Barrett, Anna E Lehmann
PURPOSE: To study the cure rate of fungal keratitis with moxifloxacin 0.3% monotherapy. METHODS: A retrospective review of patients with culture-proven fungal keratitis who initially received moxifloxacin 0.3% monotherapy was performed. RESULTS: Eleven patients with culture-proven fungal keratitis were initially treated with moxifloxacin. One case each of Curvularia and Alternaria keratitis resolved with moxifloxacin monotherapy (18%). CONCLUSIONS: Moxifloxacin may have a significant clinical therapeutic effect in a subset of patients with fungal keratitis...
May 2017: Cornea
https://www.readbyqxmd.com/read/28248430/reconstitution-of-vanadium-haloperoxidase-s-catalytic-activity-by-boric-acid-towards-a-potential-biocatalytic-role-of-boron
#19
Filipe Natalio, Stefanie Wiese, Wolfgang Brandt, Ludger Wessjohann
Boron's unusual properties inspired major advances in chemistry. In nature, the existence and importance of boron has been fairly explored (e.g. bacterial signaling, plant development) but its role as biological catalyst was never reported. Here, we show that boric acid [B(OH)3 ] can restore chloroperoxidase activity of Curvularia inaequalis recombinant apo-haloperoxidase's (HPO) in the presence of hydrogen peroxide and chloride ions. Molecular modeling and semi-empirical PM7 calculations support a thermodynamically highly favored (bio)catalytic mechanism similarly to vanadium haloperoxidases (V-HPO) in which [B(OH)3 ] is assumedly located in apo-HPO's active site and a monoperoxyborate [B(OH)3 (OOH)(-) ] intermediate is formed and stabilized by interaction with specific active site amino acids leading ultimately to the formation of HOCl...
March 1, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28204952/a-preliminary-report-of-indigenous-fungal-isolates-from-contaminated-municipal-solid-waste-site-in-india
#20
Abhishek Kumar Awasthi, Akhilesh Kumar Pandey, Jamaluddin Khan
Municipal solid waste (MSW) containing harmful substances is a major concern in waste management and can cause adverse effects on diversity of fungi in soil. The main objective was to evaluate the fungal diversity inhabiting in the soil nearby MSW disposal site. The fungal strains were isolated in potato dextrose agar (PDA), media at temperatures 28 ± 1 °C by using standard serial dilution pour plate method, and appeared fungal colonies identified based on morphological characteristics. The overall most fungal diversity was found in soil sample collected from S5, followed by S4, S3, S1, and least in S2 site...
March 2017: Environmental Science and Pollution Research International
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