Chuncui Huang, Hui Wang, Jinyu Zhou, Yikang Huang, Yihui Ren, Keli Zhao, Yaojun Wang, Meijie Hou, Jingwei Zhang, Yaming Liu, Xinyue Ma, Jingyu Yan, Dongbo Bu, Wengang Chai, Shiwei Sun, Yan Li
Biological functions of glycans are intimately linked to fine details in branches and linkages, which make structural identification extremely challenging. Here, we present a protocol for automated N-glycan sequencing using multi-stage mass spectrometry (MSn ). We describe steps for release/purification and derivation of glycans and procedures for MSn scanning. We then detail "glycan intelligent precursor selection" to computationally guide MSn experiments. The protocol can be used for both discrete individual glycans and isomeric glycan mixtures...
April 16, 2024: STAR protocols