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https://www.readbyqxmd.com/read/28938751/new-permeable-cryoprotectant-free-vitrification-method-for-native-human-sperm
#1
J Aizpurua, L Medrano, M Enciso, J Sarasa, A Romero, M A Fernández, M J Gómez-Torres
STUDY QUESTION: Is permeable cryoprotectant-free vitrification of native sperm samples a good alternative to conventional slow freezing? SUMMARY ANSWER: The permeable cryoprotectant-free sperm vitrification protocol tested in this study renders considerably better recovery rates of good quality sperm compared to slow freezing. WHAT IS KNOWN ALREADY: Slow freezing is currently the most commonly used technique for sperm cryopreservation, though this method has been repeatedly shown to have negative effects on both structural and functional sperm features...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938744/an-improved-ivm-method-for-cumulus-oocyte-complexes-from-small-follicles-in-polycystic-ovary-syndrome-patients-enhances-oocyte-competence-and-embryo-yield
#2
F Sánchez, F Lolicato, S Romero, M De Vos, H Van Ranst, G Verheyen, E Anckaert, J E J Smitz
STUDY QUESTION: Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed by a maturation step in presence of FSH and Amphiregulin (AREG)? SUMMARY ANSWER: A strategy involving prematuration culture (PMC) in the presence of CNP followed by IVM using FSH + AREG increases oocyte maturation potential leading to a higher availability of Day 3 embryos and good-quality blastocysts for single embryo transfer...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938393/ilizarov-treatment-protocols-in-the-management-of-infected-nonunion-of-the-tibia
#3
Martin McNally, Jamie Ferguson, Raj Kugan, David Stubbs
OBJECTIVES: We present a treatment algorithm comprising 4 Ilizarov methods in managing infected tibial nonunion, using nonunion mobility and segmental defect size to govern treatment choice. DESIGN: Decision protocol analysis study. SETTING: A university-affiliated teaching hospital. PATIENTS/PARTICIPANTS: Seventy-nine patients were treated with 1 of 4 Ilizarov protocols. All patients had undergone at least one previous operation, 38 had associated limb deformity, and 49 had nonviable nonunions...
October 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28938234/is-early-total-care-a-safe-and-effective-alternative-to-staged-protocol-for-the-treatment-of-schatzker-iv-vi-tibial-plateau-fractures-in-patients-older-than-50-years
#4
Amrut Borade, Harish Kempegowda, Raveesh Richard, Jove Graham, Michael Suk, Daniel S Horwitz
OBJECTIVES: To compare "Early Total Care" (ETC) with "Staged Protocol" (SP) for the treatment of Schatzker IV-VI tibial plateau fractures in patients older than 50 years regarding safety and effectiveness. DESIGN: Retrospective cohort study. SETTING: An academic level 1 US trauma center. PATIENTS/PARTICIPANTS: Eighty-one patients older than 50 years with Schatzker grade IV-VI tibial plateau fractures were included. INTERVENTION: Fifty-three patients were treated under SP with immediate external fixation followed by definitive internal fixation...
September 19, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28938202/effect-of-different-diode-laser-wavelengths-on-root-dentin-decontamination-infected-with-enterococcus-faecalis
#5
Caroline Cristina Borges, Carlos Estrela, Fabiane Carneiro Lopes, Regina Guenka Palma-Dibb, Jesus Djalma Pecora, Cyntia Rodrigues De Araújo Estrela, Manoel Damião de Sousa-Neto
The objective of this study was to evaluate the antibacterial effect and the ultrastructural alterations of diode laser with different wavelengths (808nm and 970nm) and its association with irrigating solutions (2.5% sodium hypochlorite and 2% chlorhexidine) in root dentin contaminated by a five days biofilm. Thirteen uniradicular teeth were sectioned into 100 dentin intraradicular blocks. Initially, the blocks were immersed for 5min in 17% EDTA and washed with distilled water for 5min, then samples were sterilized for 30min at 120°C...
September 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28937898/selective-reduction-of-talon-cusps-a-case-series
#6
Rona Leith, Anne C O'Connell
Talon cusps associated with permanent incisors are a rare occurrence but can cause numerous complications. When treatment is indicated options include radical or selective reduction of the accessory cusp, but there is no standard protocol for management. STUDY DESIGN: Patients referred to the Dublin Dental University Hospital for treatment of talon cusps were identified, contacted and consent obtained. Clinical notes, photographs and radiographs were retrospectively reviewed. RESULTS: Eleven patients with 14 talon cusps were identified...
September 22, 2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28937783/validation-of-the-missouri-osteochondral-allograft-preservation-system-for-the-maintenance-of-osteochondral-allograft-quality-during-prolonged-storage
#7
Aaron M Stoker, James P Stannard, Keiichi Kuroki, Chantelle C Bozynski, Ferris M Pfeiffer, James L Cook
BACKGROUND: Fresh osteochondral allografts (OCAs) are limited in availability. The Missouri Osteochondral Allograft Preservation System (MOPS) has been reported to effectively preserve OCAs twice as long as current tissue bank protocols in preclinical studies. HYPOTHESIS: The viable chondrocyte density (VCD) in OCAs preserved for up to 70 days using the MOPS will not be significantly different from day 0, and the VCD in MOPS-preserved OCAs will be significantly higher than for standard tissue bank preservation...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28937695/trinuclear-complexes-of-palladium-ii-with-chalcogenated-n-heterocyclic-carbenes-catalysis-of-selective-nitrile-primary-amide-interconversion-and-sonogashira-coupling
#8
Pooja Dubey, Sonu Gupta, Ajai K Singh
3-Methyl-1-(2-(phenylthio/seleno)ethyl)-1H-benzo[d]imidazol-3-ium iodide (L1/L2), a precursor of sulfated/selenated N-heterocyclic carbene, was synthesized by the reaction of benzimidazole with 1,2-dichloroethane followed by treatment with PhS/SeNa and MeI. The reaction of L1/L2 with Ag2O followed by treatment with [Pd(CH3CN)2Cl2] (metal to ligand ratio 3 : 2), i.e. transmetallation, resulted in trinuclear palladium(ii) complexes [Pd3(L1/L2-HI)2(CH3CN)Cl6] (1-2). The complexes were characterized with (1)H, (13)C{(1)H} and (77)Se{(1)H} NMR (2 only), elemental analyses, HR-MS and single-crystal X-ray diffraction...
September 22, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28937573/committee-opinion-no-721-smoking-cessation-during-pregnancy
#9
(no author information available yet)
Smoking is the one of the most important modifiable causes of poor pregnancy outcomes in the United States, and is associated with maternal, fetal, and infant morbidity and mortality. The physical and psychologic addiction to cigarettes is powerful; however, the compassionate intervention of the obstetrician-gynecologist can be the critical element in prenatal smoking cessation. An office-based protocol that systematically identifies pregnant women who smoke and offers treatment or referral has been proved to increase quit rates...
October 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28937568/committee-opinion-no-721-summary-smoking-cessation-during-pregnancy
#10
(no author information available yet)
Smoking is the one of the most important modifiable causes of poor pregnancy outcomes in the United States, and is associated with maternal, fetal, and infant morbidity and mortality. The physical and psychologic addiction to cigarettes is powerful; however, the compassionate intervention of the obstetrician-gynecologist can be the critical element in prenatal smoking cessation. An office-based protocol that systematically identifies pregnant women who smoke and offers treatment or referral has been proved to increase quit rates...
October 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28937374/malignant-phyllodes-tumor-with-heterologous-differentiation-clinicopathological-spectrum-of-nine-cases-in-a-tertiary-care-institute-in-eastern-india
#11
Mamita Nayak, Susama Patra, Pritinanda Mishra, Nibedita Sahoo, Prakash Kumar Sasmal, Tushar Subhadarshan Mishra
Phyllodes tumors are uncommon fibroepithelial neoplasms of breast. Heterologous sarcomatous differentiation of malignant phyllodes tumor (MPT) is a rare phenomenon as shown in the literature. Herein we report a series of nine cases from a tertiary care centre in Eastern India. Patients demographic data and clinical details were obtained from the medical records. Histopathology and immunohistochemical studies were analysed and diagnosed accordingly. Out of 38 cases of PT, 13 were found to be MPT, of which 9 cases showed heterologous differentiation in the form of angiosarcoma, fibrosarcoma, undifferentiated sarcoma, extensive squamous differentiation and with lipomatous metaplasia...
July 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28937310/remotely-supervised-transcranial-direct-current-stimulation-for-the-treatment-of-fatigue-in-multiple-sclerosis-results-from-a-randomized-sham-controlled-trial
#12
Leigh E Charvet, Bryan Dobbs, Michael T Shaw, Marom Bikson, Abhishek Datta, Lauren B Krupp
BACKGROUND: Fatigue is a common and debilitating feature of multiple sclerosis (MS) that remains without reliably effective treatment. Transcranial direct current stimulation (tDCS) is a promising option for fatigue reduction. We developed a telerehabilitation protocol that delivers tDCS to participants at home using specially designed equipment and real-time supervision (remotely supervised transcranial direct current stimulation (RS-tDCS)). OBJECTIVE: To evaluate whether tDCS can reduce fatigue in individuals with MS...
September 1, 2017: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/28937147/comparison-of-effectiveness-and-safety-between-conbercept-and-ranibizumab-for-treatment-of-neovascular-age-related-macular-degeneration-a-retrospective-case-controlled-non-inferiority-multiple-center-study
#13
J Cui, D Sun, H Lu, R Dai, L Xing, H Dong, L Wang, D Wei, B Jiang, Y Jiao, M M Jablonski, S Charles, W Gu, H Chen
PurposeTo compare the efficacy and safety of conbercept and ranibizumab when administered according to a treat-and-extend (TREX) protocol for the treatment of neovascular age-related macular degeneration (AMD) in China.Patients and methodsBetween May 2014 and May 2015, 180 patients were treated in a 1 : 1 ratio using conbercept or ranibizumab from four hospitals. Patients received either conbercept 0.5 mg or ranibizumab 0.5 mg intravitreal injections. Follow-up time was 1 year and treated based on a TREX approach...
September 22, 2017: Eye
https://www.readbyqxmd.com/read/28937044/priming-of-the-cells-hypoxic-preconditioning-for-stem-cell-therapy
#14
REVIEW
Zheng Z Wei, Yan-Bing Zhu, James Y Zhang, Myles R McCrary, Song Wang, Yong-Bo Zhang, Shan-Ping Yu, Ling Wei
OBJECTIVE: Stem cell-based therapies are promising in regenerative medicine for protecting and repairing damaged brain tissues after injury or in the context of chronic diseases. Hypoxia can induce physiological and pathological responses. A hypoxic insult might act as a double-edged sword, it induces cell death and brain damage, but on the other hand, sublethal hypoxia can trigger an adaptation response called hypoxic preconditioning or hypoxic tolerance that is of immense importance for the survival of cells and tissues...
October 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28937021/optimized-high-dose-amoxicillin-proton-pump-inhibitor-dual-therapies-fail-to-achieve-high-cure-rates-in-china
#15
Jia-Li Hu, Jun Yang, Yin-Bin Zhou, Ping Li, Ran Han, Dian-Chun Fang
Background\Aim: Quadruple daily administration of proton-pump inhibitor (PPI) therapy achieves potent acid inhibition, and combined with amoxicillin, with its pharmacodynamic and pharmacokinetic characteristics, may be efficient for Helicobacter pylori eradication. We compared the efficacy of two optimized high-dose dual therapies with a bismuth-containing quadruple regimen for treating H. pylori infection. Rabeprazole dosages for H. pylori eradication were also evaluated. PATIENTS AND METHODS: Treatment-naive and H...
September 2017: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/28936647/3d-imaging-of-whole-mount-ovules-at-cellular-resolution-to-study-female-germline-development-in-rice
#16
Ethel Mendocilla-Sato, Wenjing She, Célia Baroux
Recent advances in fluorescence-based staining of cellular compartments coupled with confocal microscopy imaging have allowed the visualization of three-dimensional (3D) structures with cellular resolution in various intact plant tissues and species. Such approaches are of particular interest for the analysis of the reproductive lineage in plants including the meiotic precursor cells deeply embedded within the ovary of the gynoecium enclosed in the flower. Yet, their relative inaccessibility and the lack of optical clarity of plant tissues prevent robust staining and imaging across several cell layers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936297/dental-management-in-patients-with-antiplatelet-therapy-a-systematic-review
#17
REVIEW
Luis-Miguel Sáez-Alcaide, Cristina Sola-Martín, Pedro Molinero-Mourelle, Victor Paredes-Rodríguez, Carmen Zarrias-Caballero, Gonzalo Hernández-Vallejo
INTRODUCTION: Cardiovascular diseases are the most frequent cause of death in the Western world. Its treatment frequently needs therapy with antiplatelet agents, which increases the haemorrhage risk after oral surgical procedures. The aim of this study is to present a review on the dental management of the patients under antiplatelet treatment. MATERIAL AND METHODS: A systematic review was carried out following PRISMA recommendations including studies searched in Pubmed-Medline, Embase and Cochrane databases...
August 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28936282/effect-of-thermocycling-and-surface-treatment-on-repair-bond-strength-of-composite
#18
Nazanin Kiomarsi, Pardis Saburian, Nasim Chiniforush, Mohammad-Javd Karazifard, Sedighe-Sadat Hashemikamangar
BACKGROUND: Repair of composite restorations is a conservative method that can increase the longevity and durability of restorations while preserving the tooth structure. Achieving a suitable bond between the old and new composite is difficult. To overcome this problem, some methods have been recommended to increase the repair bond strength of composite.This study aimed to assess the effect of aging by thermocycling (5,000 and 10,000 cycles) and mechanical surface treatments (Er,Cr:YSGG laser and bur) on repair shear bond strength of composite resin...
August 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28936070/traumatic-cerrebral-fungus-experience-from-an-institution-in-north-east-india
#19
Binoy Kumar Singh, Abinash Dutta, Shameem Ahmed, Zakir Hussain, Rajib Hazarika, Bineta Singh
BACKGROUND: Traumatic brain fungus is manifestation of neglected head injury. Although rare it is not uncommon. The patients are usually intact with good Glasgow coma (GCS) score inspite of complex injuries and exposed brain parenchyma but morbidity and mortality is very high with time if no proper and timely management is offered. There is very less study on traumatic brain fungus with no defined management protocols. So an attempt was made to explain in details the surgical strategies and other management techniques in patients with traumatic brain fungus...
August 2017: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/28935932/cannabidiol-attenuates-alcohol-induced-liver-steatosis-metabolic-dysregulation-inflammation-and-neutrophil-mediated-injury
#20
Yuping Wang, Partha Mukhopadhyay, Zongxian Cao, Hua Wang, Dechun Feng, György Haskó, Raphael Mechoulam, Bin Gao, Pal Pacher
Cannabidiol (CBD) is a non-psychoactive component of marijuana, which has anti-inflammatory effects. It has also been approved by FDA for various orphan diseases for exploratory trials. Herein, we investigated the effects of CBD on liver injury induced by chronic plus binge alcohol feeding in mice. CBD or vehicle was administered daily throughout the alcohol feeding study. At the conclusion of the feeding protocol, serums samples, livers or isolated neutrophils were utilized for molecular biology, biochemistry and pathology analysis...
September 21, 2017: Scientific Reports
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