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https://www.readbyqxmd.com/read/27906100/an-isothermal-dna-amplification-method-for-detection-of-onchocerca-volvulus-infection-in-skin-biopsies
#1
Ole Lagatie, Michelle Merino, Linda Batsa Debrah, Alexander Y Debrah, Lieven J Stuyver
BACKGROUND: Diagnostic procedures for the diagnosis of infection with the nematode parasite Onchocerca volvulus are currently based on the microscopic detection of microfilariae in skin biopsies. Alternative approaches based on amplification of parasitic DNA in these skin biopsies are currently being explored. Mostly this is based on the detection of the O-150 repeat sequence using PCR based techniques. METHODS: An isothermal, loop-mediated amplification method has been designed using the mitochondrial O...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27842567/still-mesoendemic-onchocerciasis-in-two-cameroonian-community-directed-treatment-with-ivermectin-projects-despite-more-than-15-years-of-mass-treatment
#2
Guy-Roger Kamga, Fanny N Dissak-Delon, Hugues C Nana-Djeunga, Benjamin D Biholong, Stephen Mbigha-Ghogomu, Jacob Souopgui, Honorat G M Zoure, Michel Boussinesq, Joseph Kamgno, Annie Robert
BACKGROUND: After more than a decade of community-directed treatment with ivermectin (CDTI) in Centre and Littoral Regions of Cameroon, onchocerciasis endemicity was still high in some communities according to the 2011 epidemiological evaluations. Some corrective measures were undertaken to improve the CDTI process and therefore reduce the burden of the disease. The objective of the present study was to assess the progress made towards the elimination of onchocerciasis in the Centre 1 and Littoral 2 CDTI projects where the worst performances were found in 2011...
November 14, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27814962/neural-complexity-as-a-potential-translational-biomarker-for-psychosis
#3
Brandon Hager, Albert C Yang, Roscoe Brady, Shashwath Meda, Brett Clementz, Godfrey D Pearlson, John A Sweeney, Carol Tamminga, Matcheri Keshavan
BACKGROUND: The adaptability of the human brain to the constantly changing environment is reduced in patients with psychotic disorders, leading to impaired cognitive functions. Brain signal complexity, which may reflect adaptability, can be readily quantified via resting-state functional magnetic resonance imaging (fMRI) signals. We hypothesized that resting-state brain signal complexity is altered in psychotic disorders, and is correlated with cognitive impairment. METHODS: We assessed 156 healthy controls (HC) and 330 probands, including 125 patients with psychotic bipolar disorder (BP), 107 patients with schizophrenia (SZ), 98 patients with schizoaffective disorder (SAD) and 230 of their unaffected first-degree relatives (76 BPR, 79 SADR, and 75 SZR) from four sites of the Bipolar-Schizophrenia Network on Intermediate Phenotypes (B-SNIP) consortium...
October 26, 2016: Journal of Affective Disorders
https://www.readbyqxmd.com/read/27789186/novel-gene-brain-structure-relationships-in-psychotic-disorder-revealed-using-parallel-independent-component-analyses
#4
Neeraj Tandon, Pranav Nanda, Jaya L Padmanabhan, Ian T Mathew, Shaun M Eack, Balaji Narayanan, Shashwath A Meda, Sarah E Bergen, Gualbert Ruaño, Andreas Windemuth, Mohan Kocherla, Tracey L Petryshen, Brett Clementz, John Sweeney, Carol Tamminga, Godfrey Pearlson, Matcheri S Keshavan
BACKGROUND: Schizophrenia, schizoaffective disorder, and psychotic bipolar disorder overlap with regard to symptoms, structural and functional brain abnormalities, and genetic risk factors. Neurobiological pathways connecting genes to clinical phenotypes across the spectrum from schizophrenia to psychotic bipolar disorder remain largely unknown. METHODS: We examined the relationship between structural brain changes and risk alleles across the psychosis spectrum in the multi-site Bipolar-Schizophrenia Network for Intermediate Phenotypes (B-SNIP) cohort...
October 24, 2016: Schizophrenia Research
https://www.readbyqxmd.com/read/27751623/phrenic-nerve-stimulation-is-more-sensitive-than-ultrasound-measurement-of-diaphragm-thickness-in-assessing-early-als-progression
#5
Susana Pinto, Pedro Alves, Michael Swash, Mamede de Carvalho
AIMS OF THE STUDY: To compare the assessment of respiratory decline with conventional tests, measurement of diaphragm compound muscle action potential (CMAP) to phrenic nerve stimulation and diaphragm thickness to ultrasound (US) investigation in amyotrophic lateral sclerosis (ALS) patients followed for a short period of time. PATIENTS AND METHODS: We evaluated in 40 consecutive ALS patients, the clinical functional scale (ALSFRS-R), forced vital capacity (FVC), maximal voluntary ventilation (MVV), maximal inspiratory (MIP) and expiratory (MEP) pressures, sniff nasal inspiratory pressure (SNIP), Diaphragm-CMAP (latency and amplitude) and diaphragm US (maximal and minimal diaphragm thickness during full inspiration and expiration, respectively)...
October 14, 2016: Neurophysiologie Clinique, Clinical Neurophysiology
https://www.readbyqxmd.com/read/27698270/onchocerciasis-elimination-from-africa-one-step-in-northern-sudan
#6
James W Kazura
Onchocerciasis is one of the two filarial helminth "neglected tropical diseases" (the other being lymphatic filariasis) that has been targeted for geographically local elimination followed by global eradication. The last known areas of Onchocerca volvulus transmission in the Americas have recently been reported to be eliminated. In contrast, achieving metrics for interruption of O. volvulus transmission in Africa, thus removing the requirement for continued monitoring and mass drug administration (MDA) with ivermectin, has been more challenging...
October 3, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27695592/the-western-snip-stitch-and-tug-hydrocelectomy-how-i-do-it
#7
Neal E Rowe, Paul Martin, Patrick P Luke
INTRODUCTION: Adult idiopathic hydrocele is a common benign disorder that merits surgical correction when symptomatic. The most popular techniques for repair are plication (Lord's procedure) or excision and eversion of the tunica vaginalis (Jaboulay procedure). Established complications from these traditional repairs include hematoma, recurrence, and infection. These procedures are performed through a scrotal incision. We describe a novel technique of hydrocele repair with gubernaculum preservation through a subinguinal incision...
September 2016: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/27648069/epidemiological-mapping-of-human-onchocerciasis-in-transmission-suspected-districts-of-bale-borena-and-west-arsi-zones-of-eastern-ethiopia
#8
Sindew Mekasha Feleke, Gemechu Tadesse, Kalkidan Mekete, Afework Hailemariam Tekle, Amha Kebede
Onchocerciasis is mainly found in western part of Ethiopia and there is no evidence of transmission in the east ward. However, some zones (Bale, Borena, and West Arsi) are suspected for transmission given the area has fast flowing rivers and is covered with vegetation. Therefore, this study was conducted to map onchocerciasis transmission in those zones. About 19 villages were selected based on proximity to the rivers, representation of districts, zones, and vegetation covers, whereas the study participants, all village residents of age > 5 years with good health condition, were skin sniped and examined using microscopy...
2016: Interdisciplinary Perspectives on Infectious Diseases
https://www.readbyqxmd.com/read/27624093/inorganic-double-helices-in-semiconducting-snip
#9
Daniela Pfister, Konrad Schäfer, Claudia Ott, Birgit Gerke, Rainer Pöttgen, Oliver Janka, Maximilian Baumgartner, Anastasia Efimova, Andrea Hohmann, Peer Schmidt, Sabarinathan Venkatachalam, Leo van Wüllen, Ulrich Schürmann, Lorenz Kienle, Viola Duppel, Eric Parzinger, Bastian Miller, Jonathan Becker, Alexander Holleitner, Richard Weihrich, Tom Nilges
SnIP is the first atomic-scale double helical semiconductor featuring a 1.86 eV band gap, high structural and mechanical flexibility, and reasonable thermal stability up to 600 K. It is accessible on a gram scale and consists of a racemic mixture of right- and left-handed double helices composed by [SnI] and [P] helices. SnIP nanorods <20 nm in diameter can be accessed mechanically and chemically within minutes.
September 14, 2016: Advanced Materials
https://www.readbyqxmd.com/read/27616192/a-randomized-clinical-trial-on-the-comparison-between-hair-shaving-and-snipping-prior-to-laser-hair-removal-sessions-in-women-suffering-from-hirsutism
#10
Robabeh Alijanpour, Fatemeh Aliakbarpour
BACKGROUND AND OBJECTIVES: Hirsutism or excess hair growth is a common dermatological problem in females, affecting up to 10% of females worldwide. We aimed this study to compare the two hair-shortening methods shaving vs. snipping among hirsute women regarding the outcome of the therapy and also the paradoxical hypertrichosis phenomenon (terminal hair regrowth) 6 months after the final therapy session. MATERIALS AND METHODS: In this study, 129 premenopausal women affected by hirsutism were randomly divided into two study groups according to hair-shortening method: shaving as group A (66 patients) and hair shortening by scissor as group B (63 patients)...
September 11, 2016: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/27604904/paramyosin-of-canine-onchocerca-lupi-usefulness-for-the-diagnosis-of-a-neglected-zoonotic-disease
#11
Bronwyn Campbell, Helder Cortes, Giada Annoscia, Alessio Giannelli, Antonio Parisi, Maria Stefania Latrofa, Filipe Dantas-Torres, Luís Cardoso, Domenico Otranto
BACKGROUND: Of increasing importance to the medical and veterinary communities is the zoonotic filarioid nematode Onchocerca lupi. Onchocercosis, thus far found in wolves, dogs, cats and humans, is diagnosed via skin snips to detect microfilariae and surgical removal of adults from the eye of the host. These methods are time-consuming, laborious and invasive, highlighting the need for new tools for the diagnosis of O. lupi in susceptible hosts. Symptoms related to the presence of the adults in the eye can range from none apparent to severe, including blindness...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27561958/functional-outcome-of-total-knee-arthroplasty-in-patients-with-haemophilia
#12
S M J Mortazavi, B Haghpanah, M M Ebrahiminasab, T Baghdadi, G Toogeh
INTRODUCTION: Haemophilic arthropathy (HA) is a debilitating complication of haemophilia which leads to TKA in severe cases. AIM: We conducted a prospective study of the outcome of TKA in our haemophilia cohort to define the outcomes in this population and increase the cost effectiveness of the procedure in our developing country. METHODS: We reviewed patients with haemophilia who underwent TKA between April 2010 and April 2014. Patients with at least 6 months of follow-up were included...
November 2016: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/27557563/prediction-of-the-originating-site-of-sinonasal-inverted-papilloma-by-preoperative-magnetic-resonance-imaging-and-computed-tomography
#13
Gaoli Fang, Hongfei Lou, Wenling Yu, Xiangdong Wang, Bentao Yang, Junfang Xian, Xiaohong Song, Erzhong Fan, Ying Li, Chengshuo Wang, Luo Zhang
BACKGROUND: Precise localization and excision of the originating site of a sinonasal inverted papilloma (SNIP) is essential for decreasing tumor recurrence. In this study we evaluated the use of preoperative computed tomography (CT) and magnetic resonance imaging (MRI) to pinpoint the attachment/originating sites of SNIPs in 143 patients. METHODS: Osteitis signs in CTs and convoluted cerebriform pattern (CCP)-based reverse tracings from MRIs of 143 SNIP patients were analyzed preoperatively to predict the originating site of SNIPs...
August 25, 2016: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/27514878/filarioids-infecting-dogs-in-northeastern-brazil
#14
Rafael Antonio Nascimento Ramos, Ana Gabriela de Oliveira do Rêgo, Everton Diogo de Farias Firmino, Carlos Alberto do Nascimento Ramos, Gílcia Aparecida de Carvalho, Filipe Dantas-Torres, Domenico Otranto, Leucio Câmara Alves
Species of filarial nematodes belonging to the genera Dirofilaria and Acanthocheilonema are recognised as common parasites of dogs throughout the world. Recently, other filarioids featured by the presence of dermal microfilariae (e.g., Onchocerca lupi and Cercopithifilaria spp.) have been recognised in Europe. In Brazil, reports of filarioids in dogs are limited to Dirofilaria immitis, Acanthocheilonema reconditum and Cercopithifilaria bainae. To investigate the distribution of filarial infections in dogs living in an endemic region from northeastern Brazil, blood and skin samples (n=104) were microscopically (modified Knott's test and skin snip sediment examination) and molecularly evaluated...
August 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27477140/challenges-facing-the-identification-of-neurobiologically-distinct-psychosis-subtypes-response-to-neuhaus
#15
Brett A Clementz
No abstract text is available yet for this article.
August 1, 2016: American Journal of Psychiatry
https://www.readbyqxmd.com/read/27473357/comparison-of-a-new-visual-isothermal-nucleic-acid-amplification-test-with-pcr-and-skin-snip-analysis-for-diagnosis-of-onchocerciasis-in-humans
#16
Andy Alhassan, Mike Y Osei-Atweneboana, Kwadwo F Kyeremeh, Catherine B Poole, Zhiru Li, Edward Tettevi, Nathan A Tanner, Clotilde K S Carlow
Accurate, simple and affordable diagnostics are needed to detect Onchocerca volvulus infection in humans. A newly developed colorimetric loop-mediated isothermal amplification (LAMP) assay was compared to PCR and skin snip analysis for diagnosis of onchocerciasis. The robustness and simplicity of the assay indicates that it may be a useful field tool for surveillance in endemic countries.
July 26, 2016: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/27473047/the-impact-of-socioeconomic-factors-on-the-efficiency-of-voluntary-toxoplasmosis-screening-during-pregnancy-a-population-based-study
#17
A E Lange, J R Thyrian, S Wetzka, S Flessa, W Hoffmann, M Zygmunt, C Fusch, H N Lode, M Heckmann
BACKGROUND: Congenital toxoplasmosis is associated with severe complications. German state health insurance covers rubella, but not toxoplasmosis, immunity screening. We analysed the effect of socioeconomic factors on the efficiency of private toxoplasmosis screening during pregnancy. METHODS: Toxoplasmosis and rubella screening data (n = 5402 mothers) were collected within the population-based Survey of Neonates in Pomerania (SNiP). RESULTS: At the first-trimester screening, 34...
2016: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/27469307/diffusion-weighted-mri-abnormalities-in-an-outbreak-of-streptococcus-agalactiae-serotype-iii-multilocus-sequence-type-283-meningitis
#18
Kevin Tan, Limin Wijaya, Hui-Jin Chiew, Yih-Yian Sitoh, Humaira Shafi, Robert C Chen, Chin Kong Goh, C C Tchoyoson Lim
PURPOSE: In 2015, an outbreak of group B streptococcal (GBS) infection caused by Streptococcus agalactiae Serotype III, multilocus sequence type 283, related to consuming infected raw freshwater fish, affected more than 200 patients in Singapore. We describe the clinical, laboratory, and neuroimaging features of a subgroup of adults with central nervous system (CNS) infections caused by GBS. MATERIALS AND METHODS: The database of the Singapore Neurologic Infections Program (SNIP), a national multicenter study for surveillance of infectious neurologic disease, was reviewed to select patients with GBS CNS infection during the outbreak...
July 29, 2016: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/27449914/reference-range-of-respiratory-muscle-strength-and-its-clinical-application-in-amyotrophic-lateral-sclerosis-a-single-center-study
#19
Kee Hong Park, Rock Bum Kim, Jiwon Yang, Jung Hwan Oh, Su Yeon Park, Dong Gun Kim, Je Young Shin, Jung Joon Sung
BACKGROUND AND PURPOSE: Evaluating respiratory function is important in neuromuscular diseases. This study explored the reference ranges of the maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), and sniff nasal inspiratory pressure (SNIP) in healthy adults, and applied them to amyotrophic lateral sclerosis (ALS) patients. METHODS: MIP, MEP, and SNIP were measured in 67 healthy volunteers aged from 21 to 82 years. Reference ranges were evaluated by multivariate regression analysis using the generalized additive modeling of location, scale, and shape method...
July 2016: Journal of Clinical Neurology
https://www.readbyqxmd.com/read/27448085/evidence-for-suppression-of-onchocerciasis-transmission-in-bioko-island-equatorial-guinea
#20
Laura Moya, Zaida Herrador, Thuy Huong Ta-Tang, Jose Miguel Rubio, Maria Jesús Perteguer, Ana Hernandez-González, Belén García, Rufino Nguema, Justino Nguema, Policarpo Ncogo, Teresa Garate, Agustín Benito, Anacleto Sima, Pilar Aparicio
Onchocerciasis or "river blindness" is a chronic parasitic neglected tropical disease which is endemic both in mainland and insular Equatorial Guinea. We aim to estimate the current epidemiological situation of onchocerciasis in Bioko Island after vector elimination in 2005 and more than sixteen years of Community Directed Treatment with Ivermectin (CDTI) by using molecular and serological approaches for onchocerciasis diagnosis. A community-based cross-sectional study was carried out in Bioko Island from mid-January to mid-February 2014...
July 2016: PLoS Neglected Tropical Diseases
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