Jianfei Yan, Bo Gao, Chenyu Wang, Weicheng Lu, Wenpin Qin, Xiaoxiao Han, Yingying Liu, Tao Li, Zhenxing Guo, Tao Ye, Qianqian Wan, Haoqing Xu, Junjun Kang, Naining Lu, Changhe Gao, Zixuan Qin, Chi Yang, Jisi Zheng, Pei Shen, Lina Niu, Weiguo Zou, Kai Jiao
Heterotopic ossification (HO) comprises the abnormal formation of ectopic bone in extraskeletal soft tissue. The factors that initiate HO remain elusive. Herein, we found that calcified apoptotic vesicles (apoVs) led to increased calcification and stiffness of tendon extracellular matrix (ECM), which initiated M2 macrophage polarization and HO progression. Specifically, single-cell transcriptome analyses of different stages of HO revealed that calcified apoVs were primarily secreted by a PROCR+ fibroblast population...
April 2024: Journal of Extracellular Vesicles