Pedro Martín, Melisa Moncada, Karen Castillo, Federico Orsi, Gerónimo Ducca, José Manuel Fernández-Fernández, Carlos Gonzalez, Verónica Milesi
Arachidonic acid (AA) is a fatty acid involved in the modulation of several ion channels. Previously, we reported that AA activates the high conductance Ca2+ - and voltage-dependent K+ channel (BK) in vascular smooth muscle depending on the expression of the auxiliary β1 subunit. Here, using the patch-clamp technique on BK channel co-expressed with β1 subunit in a heterologous cell expression system, we analyzed whether AA modifies the three functional modules involved in the channel gating: the voltage sensor domain (VSD), the pore domain (PD), and the intracellular calcium sensor domain (CSD)...
January 5, 2021: Biochimica et Biophysica Acta. Biomembranes