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https://www.readbyqxmd.com/read/27929413/exosome-proteome-of-u-87mg-glioblastoma-cells
#1
Sohyun Chun, Seunghyun Ahn, Chang-Hwan Yeom, Seyeon Park
Exosomes are small membrane vesicles between 30 and 100 nm in diameter secreted by many cell types, and are associated with a wide range of physiological and/or pathological processes. Exosomes containing proteins, lipids, mRNA, and microRNA contribute to cell-to-cell communication and cell-to-environment regulation, however, their biological functions are not yet fully understood. In this report, exosomes in the glioblastoma cell line, U-87MG, were isolated and the proteome was investigated. In addition, exosome proteome changes in U-87MG cells exposed to a low temperature were investigated to elucidate whether the exosome proteome could respond to an external stimulus...
December 6, 2016: Biology
https://www.readbyqxmd.com/read/27927999/elevated-temperature-drives-a-shift-from-selfing-to-outcrossing-in-the-insect-pollinated-legume-faba-bean-vicia-faba
#2
Jacob Bishop, Hannah E Jones, Donal M O'Sullivan, Simon G Potts
Climate change can threaten the reproductive success of plants, both directly, through physiological damage during increasingly extreme weather events, and indirectly, through disruption of plant-pollinator interactions. To explore how plant-pollinator interactions are modified by extreme weather, we exposed faba bean (Vicia faba) plants to elevated temperature for 5 d during flowering, simulating a heatwave. We then moved the plants to flight cages with either bumblebees or no pollinators, or to two field sites, where plants were enclosed in mesh bags or pollinated by wild insect communities...
December 7, 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27903627/the-role-of-the-antioxidant-system-during-intense-endurance-exercise-lessons-from-migrating-birds
#3
REVIEW
Clara Cooper-Mullin, Scott R McWilliams
During migration, birds substantially increase their metabolic rate and burn fats as fuel and yet somehow avoid succumbing to overwhelming oxidative damage. The physiological means by which vertebrates such as migrating birds can counteract an increased production of reactive species (RS) are rather limited: they can upregulate their endogenous antioxidant system and/or consume dietary antioxidants (prophylactically or therapeutically). Thus, birds can alter different components of their antioxidant system to respond to the demands of long-duration flights, but much remains to be discovered about the complexities of RS production and antioxidant protection throughout migration...
December 1, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27900518/the-reconstruction-algorithm-used-for-68-ga-psma-hbed-cc-pet-ct-reconstruction-significantly-influences-the-number-of-detected-lymph-node-metastases-and-coeliac-ganglia
#4
Thomas Krohn, Anita Birmes, Oliver H Winz, Natascha I Drude, Felix M Mottaghy, Florian F Behrendt, Frederik A Verburg
PURPOSE: To investigate whether the numbers of lymph node metastases and coeliac ganglia delineated on [(68)Ga]PSMA-HBED-CC PET/CT scans differ among datasets generated using different reconstruction algorithms. METHODS: Data were constructed using the BLOB-OS-TF, BLOB-OS and 3D-RAMLA algorithms. All reconstructions were assessed by two nuclear medicine physicians for the number of pelvic/paraaortal lymph node metastases as well the number of coeliac ganglia. Standardized uptake values (SUV) were also calculated in different regions...
November 29, 2016: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/27862686/effect-of-castration-at-10-months-of-age-on-growth-physiology-and-behavior-of-male-feral-beef-cattle
#5
Javier Álvarez-Rodríguez, Pere Albertí, Guillermo Ripoll, Isabel Blasco, Albina Sanz
This study compared the growth performance, plasma testosterone and cortisol levels around castration at 10 months of age, and plasma insulin-like growth factor (IGF)-I concentration and flight speed, in intact bulls and steers from 10 to 21 months of age in a feral Spanish breed. Fourteen bulls (366.5 ± 48.5 kg live weight) were assigned at random to one of two treatments: surgically castrated (steers) or intact (bulls), and submitted to an identical fattening period. Steers reared until heavy live weights (21 months of age) grew slowly and had lower plasma IGF-I concentrations than intact bulls...
November 11, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27852783/an-array-of-descending-visual-interneurons-encoding-self-motion-in-drosophila
#6
Marie P Suver, Ainul Huda, Nicole Iwasaki, Steve Safarik, Michael H Dickinson
: The means by which brains transform sensory information into coherent motor actions is poorly understood. In flies, a relatively small set of descending interneurons are responsible for conveying sensory information and higher-order commands from the brain to motor circuits in the ventral nerve cord. Here, we describe three pairs of genetically identified descending interneurons that integrate information from wide-field visual interneurons and project directly to motor centers controlling flight behavior...
November 16, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27852453/current-use-and-outcomes-of-helicopter-transport-in-pediatric-trauma-a-review-of-18-291-transports
#7
Brian R Englum, Kristy L Rialon, Jina Kim, Mark L Shapiro, John E Scarborough, Henry E Rice, Obinna O Adibe, Elisabeth T Tracy
PURPOSE: The role of helicopter emergency medical services (HEMS) in pediatric trauma remains controversial. We examined its use in pediatric trauma and its effectiveness in children with moderate/severe injuries. METHODS: All blunt/penetrating trauma patients ≤18years old in the National Trauma Data Bank were evaluated for use of HEMS and in-hospital mortality. In a comparative effectiveness study, only patients treated at level I/II pediatric centers with injury severity score (ISS)≥9 were included...
October 27, 2016: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27849510/g-induced-visual-symptoms-in-a-military-helicopter-pilot
#8
Terry W McMahon, David G Newman
INTRODUCTION: Military helicopters are increasingly agile and capable of producing significant G forces experienced in the longitudinal (z) axis of the body in a head-to-foot direction (+Gz). Dehydration and fatigue can adversely affect a pilot's +Gz tolerance, leading to +Gz-induced symptomatology occurring at lower +Gz levels than expected. The potential for adverse consequences of +Gz exposure to affect flight safety in military helicopter operations needs to be recognized. This case report describes a helicopter pilot who experienced +Gz-induced visual impairment during low-level flight...
November 2016: Military Medicine
https://www.readbyqxmd.com/read/27846990/characterization-of-conformers-and-dimers-of-antithrombin-by-capillary-electrophoresis-quadrupole-time-of-flight-mass-spectrometry
#9
Anne-Lise Marie, Elena Dominguez-Vega, François Saller, Jean-Luc Plantier, Rémi Urbain, Delphine Borgel, N Thuy Tran, Govert W Somsen, Myriam Taverna
Antithrombin (AT) is a plasma glycoprotein which possesses anticoagulant and anti-inflammatory properties. AT exhibits various forms, among which are native, latent and heterodimeric ones. We studied the potential of capillary electrophoresis-mass spectrometry (CE-MS) using a sheath liquid interface, electrospray ionization (ESI), and a quadrupole-time-of-flight (Q-TOF) mass spectrometer to separate and quantify the different AT forms. For CE separation, a neutral polyvinyl alcohol (PVA) coated capillary was employed...
December 1, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27844333/cardiac-autonomic-innervation
#10
Hina K Jamali, Fahad Waqar, Myron C Gerson
The autonomic nervous system plays a key role in regulating changes in the cardiovascular system and its adaptation to various human body functions. The sympathetic arm of the autonomic nervous system is associated with the fight and flight response, while the parasympathetic division is responsible for the restorative effects on heart rate, blood pressure, and contractility. Disorders involving these two divisions can lead to, and are seen as, a manifestation of most common cardiovascular disorders. Over the last few decades, extensive research has been performed establishing imaging techniques to quantify the autonomic dysfunction associated with various cardiovascular disorders...
November 14, 2016: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/27833821/hypergravity-hinders-axonal-development-of-motor-neurons-in-caenorhabditis-elegans
#11
Saraswathi Subbammal Kalichamy, Tong Young Lee, Kyoung-Hye Yoon, Jin Il Lee
As space flight becomes more accessible in the future, humans will be exposed to gravity conditions other than our 1G environment on Earth. Our bodies and physiology, however, are adapted for life at 1G gravity. Altering gravity can have profound effects on the body, particularly the development of muscles, but the reasons and biology behind gravity's effect are not fully known. We asked whether increasing gravity had effects on the development of motor neurons that innervate and control muscle, a relatively unexplored area of gravity biology...
2016: PeerJ
https://www.readbyqxmd.com/read/27832595/psychosocial-stress-alters-the-strength-of-reticulospinal-input-to-the-human-upper-trapezius
#12
Ryan J Marker, Serge Campeau, Katrina Shaun Maluf
Psychosocial stress has been shown to influence several aspects of human motor control associated with the fight-or-flight response, including augmentation of upper trapezius muscle activity. Given the established role of the reticular formation in arousal, this study investigated the contribution of reticulospinal activation to trapezius muscle activity during exposure to an acute psychosocial stressor. Twenty-five healthy adults were exposed to startling acoustic stimuli (SAS) while performing a motor task during periods of low and high psychosocial stress...
November 2, 2016: Journal of Neurophysiology
https://www.readbyqxmd.com/read/27827404/flight-behaviour-of-honey-bee-apis-mellifera-workers-is-altered-by-initial-infections-of-the-fungal-parasite-nosema-apis
#13
Ryan Dosselli, Julia Grassl, Andrew Carson, Leigh W Simmons, Boris Baer
Honey bees (Apis mellifera) host a wide range of parasites, some being known contributors towards dramatic colony losses as reported over recent years. To counter parasitic threats, honey bees possess effective immune systems. Because immune responses are predicted to cause substantial physiological costs for infected individuals, they are expected to trade off with other life history traits that ultimately affect the performance and fitness of the entire colony. Here, we tested whether the initial onset of an infection negatively impacts the flight behaviour of honey bee workers, which is an energetically demanding behaviour and a key component of foraging activities...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27823631/pharmacokinetics-and-pharmacodynamics-study-of-rhein-treating-renal-fibrosis-based-on-metabonomics-approach
#14
Hao Sun, Guangwen Luo, Zheng Xiang, Xiaojun Cai, Dahui Chen
BACKGROUND: The selection of effect indicators in the pharmacokinetic/ pharmacodynamic study of complex diseases to describe the relationship between plasma concentration and effect indicators is difficult. PURPOSE: Three effect indicators of renal fibrosis were successfully determined. The relationship between pharmacokinetics and pharmacodynamics of rhein in rhubarb was elucidated. STUDY DESIGN: The study was a metabolomics analysis of rat plasma and pharmacokinetics/ pharmacodynamics of rhein...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27813687/the-effects-of-a-transcontinental-flight-on-markers-of-coagulation-and-fibrinolysis-in-healthy-men-after-vigorous-physical-activity
#15
Brian R Kupchak, William J Kraemer, David R Hooper, Cathy Saenz, Lexie L Dulkis, Paul J Secola, Lee E Brown, Andrew J Galpin, Jared W Coburn, William H DuPont, Lydia K Caldwell, Jeff S Volek, Carl M Maresh
PURPOSE: Athletes and military service members are known to undergo strenuous exercise and sometimes have to take long haul flights soon afterwards; however, its combined effect on many physiological functions is relatively unknown. Therefore, we examined the combined effects of a full-body muscle-damaging workout and transcontinental flight on coagulation and fibrinolysis in healthy, resistance trained men. We also determined the efficacy of a full-body compression garment in limiting their coagulation responses...
November 4, 2016: Chronobiology International
https://www.readbyqxmd.com/read/27811296/structure-of-the-thoracic-spiracular-valves-and-their-contribution-to-the-unidirectional-gas-exchange-in-flying-blowflies-calliphora-vicina
#16
Lutz T Wasserthal, Anja S Fröhlich
The operation of the thoracic spiracular valves was analysed using anatomical and physiological techniques. Dense spiracular filter trichomes impede a diffusive gas exchange. However, the hinged posterior filter flap of the metathoracic spiracle (Sp2) opens passively during upstroke of the wings and closes by the suction of the sub-atmospheric tracheal pressure during the down stroke, which supports a unidirectional respiratory airflow. The action of the interior spiracular valve lids was recorded by photocell-sensors oriented above the enlarged spiracles and projected onto the screen of a video camera...
November 3, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27810105/does-the-gravity-orientation-of-saccular-aneurysms-influence-hemodynamics-an-experimental-study-with-and-without-flow-diverter-stent
#17
Kamil J Chodzyński, Omer F Eker, Axel E Vanrossomme, Daniel Ribeiro de Sousa, Grégory Coussement, Luc Vanhamme, Frank Dubois, Alain Bonafé, Bastien Chopard, Guy Courbebaisse, Karim Zouaoui Boudjeltia
Most intracranial aneurysms morphologic studies focused on characterization of size, location, aspect ratio, relationship to the surrounding vasculature and hemodynamics. However, the spatial orientation with respect to the gravity direction has not been taken into account although it could trigger various hemodynamic conditions. The present work addresses this possibility. It was divided in two parts: 1) the orientations of 18, 3D time-of-flight MRI (3D TOF MRI), scans of saccular aneurysms were analyzed. This investigation suggested that there was no privileged orientation for cerebral aneurysms...
October 18, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/27807928/multiparameter-single-cell-profiling-of-airway-inflammatory-cells
#18
Yi Yao, Tobias Welp, Qing Liu, Naiqian Niu, Xiaomei Wang, Clemente J Britto, Smita Krishnaswamy, Geoff L Chupp, Ruth R Montgomery
Airway diseases affect over 7% of the U.S. population and millions of patients worldwide. Asthmatic patients have wide variation in clinical severity with different clinical and physiologic manifestations of disease that may be driven by distinct biologic mechanisms. Further, the immunologic underpinnings of this complex trait disease are heterogeneous and treatment success depends on defining subgroups of asthmatics. Due to the limited availability and number of cells from the lung, the active site, in-depth investigation has been challenging...
November 3, 2016: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27788581/environmental-tobacco-smoke-alters-metabolic-systems-in-adult-rats
#19
Dinesh Kumar Barupal, Kent E Pinkerton, Carol Hood, Tobias Kind, Oliver Fiehn
Human exposure to environmental tobacco smoke (ETS) is associated with an increased incidence of pulmonary and cardiovascular disease and possibly lung cancer. Metabolomics can reveal changes in metabolic networks in organisms under different physio-pathological conditions. Our objective was to identify spatial and temporal metabolic alterations with acute and repeated subchronic ETS exposure to understand mechanisms by which ETS exposure may cause adverse physiological and structural changes in the pulmonary and cardiovascular systems...
October 28, 2016: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/27785765/long-term-effects-of-good-handling-practices-during-the-pre-weaning-period-of-crossbred-dairy-heifer-calves
#20
Luciana Pontes Silva, Aline Cristina Sant'Anna, Lívia Carolina Magalhães Silva, Mateus José Rodrigues Paranhos da Costa
The aim of this study was to determine whether applying good practices of handling during the pre-weaning period have long-term effects on behavioral and physiological indicators, health status, and average daily gain (ADG) of crossbred Bos taurus × Bos indicus heifer calves. During the pre-weaning period, 98 crossbred of Holstein × Gir heifer calves were allotted into three treatments: (1) good practices of handling + brushing (GPB; n = 25), (2) good practices of handling (GP; n = 25), and (3) control (n = 48)...
October 26, 2016: Tropical Animal Health and Production
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