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https://www.readbyqxmd.com/read/27685868/the-cell-division-control-protein-42-src-family-kinase-neural-wiskott-aldrich-syndrome-protein-pathway-regulates-human-proplatelet-formation
#1
Alberta Palazzo, Olivier Bluteau, Kahia Messaoudi, Francesco Marangoni, Yunhua Chang, Sylvie Souquere, Gérard Pierron, Valérie Lapierre, Yi Zheng, William Vainchenker, Hana Raslova, Najet Debili
BACKGROUND: Cytoskeleton rearrangements are essential in platelet release. The RHO small GTPase family, as regulators of the actin cytoskeleton, plays an important function in proplatelet formation. In the neuronal system, CDC42 is involved in the axone formation, a process that combined elongation and branching as proplatelet formation. OBJECTIVE: To analyze the role of CDC42 and its effectors of the WASP family on proplatelet formation METHODS: Human MKs were obtained from CD34(+) cells...
September 29, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27029899/association-between-tensin-1-and-p130cas-at-focal-adhesions-links-actin-inward-flux-to-cell-migration
#2
Zhihai Zhao, Song Hui Tan, Hiroaki Machiyama, Keiko Kawauchi, Keigo Araki, Hiroaki Hirata, Yasuhiro Sawada
Cell migration is a highly dynamic process that plays pivotal roles in both physiological and pathological processes. We have previously reported that p130Cas supports cell migration through the binding to Src as well as phosphorylation-dependent association with actin retrograde flow at focal adhesions. However, it remains elusive how phosphorylated Cas interacts with actin cytoskeletons. We observe that the actin-binding protein, tensin 1, co-localizes with Cas, but not with its phosphorylation-defective mutant, at focal adhesions in leading regions of migrating cells...
2016: Biology Open
https://www.readbyqxmd.com/read/26877532/p-222-effect-of-chick-peas-protein-hyrolysat-on-lipid-composition-and-peroxidation-of-hdl
#3
S Yahia, S Benomar, F Dehiba, A Allaoui, M J Rodriguez-Yoldi, J Osada, A Boualga
OBJECTIFS: Le but de ce travail est d'étudier l'effet des protéines de pois chiche hydrolysées (HPC) sur la composition lipidique et la peroxydation lipidique des HDL chez les rats hypercholestérolémiques. MéTHODES: Des rats mâles Wistar (220±10g) consommant un régime à 20 % de caséine avec 1 % de cholestérol et 0,5 % d'acide cholique sont divisés en trois lot de 6 rats reçoivent pendant 30 jours par gavage 1g/kg une solution de HPC préparées à DH = 8 % (HPC8) ou DH = 17 % (HPC17), le troisième reçoit de l'eau et constitue le groupe control (GC)...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877529/p-220-sardine-protein-compared-to-casein-combined-with-sardine-oil-or-milk-lipids-modulate-differently-dyslipidemia-and-lipid-peroxidation-of-tissues-and-lipoproteins-in-rats-fed-an-atherogenic-diet
#4
A Benyahia Mostefaoui, M Y Lamri Senhadji
OBJECTIFS: Voir si les protéines de sardine comparées à la caséine, combinées aux lipides du lait ou à l'huile de sardine peuvent moduler différemment la dyslipidémie et atténuer le stress oxydatif induit par un régime athérogène. MéTHODES: Des rats mâles (n=24) pesant 200±10g sont divisés en 4 groupes et reçoivent pendant 28 jours, 20 % de protéines de sardine ou de caséine combinées à 5 % d'huile de sardine (PsHS et casHS) ou de lipides du lait (PsLL et casLL). Les 4 régimes sont supplémentés avec 1 % de cholestérol et 0,5 % d'acide cholique...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877528/p-221-sardine-and-bogue-protein-hydrolysates-protect-aorta-by-improving-antitherogenic-propertie-of-hdl-in-hypercholesterolemic-rat
#5
S Benomar, S Yahya, F Dehiba, A Allaoui, M J Rodriguez-Yoldi, J Osada, A Boualga
OBJECTIFS: Il est bien établi que les lipoprotéines jouent un rôle important dans le processus de l'athérosclérose et particulièrement les HDL qui agissent contre cette pathologie vasculaire. Le but de ce travail est d'étudier si les hydrolysats de protéines de sardine (HPS) ou de bogue (HPB) protègent l'aorte en améliorant les propriétés antioxydantes des HDL chez le rat hypercholesterolemique. MéTHODES: 18 rats mâles Wistar (210±10 g), répartis en trois groupes de six rats chacun, consommant durant 28 jours un régime à 20 % de caséine supplémenté avec 1 % de cholestérol alimentaire et 0,5 % d'acide cholique...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26877498/p-190-effect-of-olive-cake-on-antioxidant-enzymes-activities-of-tissues-in-rats-fed-a-cholesterol-enriched-diet
#6
S Bouderbala, M Al-Hiti Kn, M Bouchenak
OBJECTIFS: L'effet des grignons d'olives (GO) après extraction d'huile est étudié sur l'activité antioxydante tissulaire chez des rats consommant un régime enrichi en cholestérol. MéTHODES: Des rats mâles de souche Wistar pesant 80±5g (n=24) sont soumis pendant 28 jours à un régime contenant 20 % de caséine + 1 % de cholestérol (HC) supplémenté ou non avec les grignons d'olives à 2,5 %, 5 % et 7,5 % (HC-GO2,5, HC-GO5 et HC-GO7,5, respectivement). RéSULTATS: Les rats hypercholestérolémiques soumis aux régimes supplémentés en GO sont comparés au groupe HC...
December 2015: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/26702143/characterization-of-the-sex-dependent-myocardial-s-nitrosothiol-proteome
#7
Qin Shao, Jonathan Fallica, Kevin M Casin, Elizabeth Murphy, Charles Steenbergen, Mark J Kohr
Premenopausal women exhibit endogenous cardioprotective signaling mechanisms that are thought to result from the beneficial effects of estrogen, which we have shown to increase protein S-nitrosylation in the heart. S-nitrosylation is a labile protein modification that increases with a number of different forms of cardioprotection, including ischemic preconditioning. Herein, we sought to identify a potential role for protein S-nitrosylation in sex-dependent cardioprotection. We utilized a Langendorff-perfused mouse heart model of ischemia-reperfusion injury with male and female hearts, and S-nitrosylation-resin-assisted capture with liquid chromatography tandem mass spectrometry to identify S-nitrosylated proteins and modification sites...
February 15, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/25990721/ap-endonuclease-1-prevents-trinucleotide-repeat-expansion-via-a-novel-mechanism-during-base-excision-repair
#8
Jill M Beaver, Yanhao Lai, Meng Xu, Astrid H Casin, Eduardo E Laverde, Yuan Liu
Base excision repair (BER) of an oxidized base within a trinucleotide repeat (TNR) tract can lead to TNR expansions that are associated with over 40 human neurodegenerative diseases. This occurs as a result of DNA secondary structures such as hairpins formed during repair. We have previously shown that BER in a TNR hairpin loop can lead to removal of the hairpin, attenuating or preventing TNR expansions. Here, we further provide the first evidence that AP endonuclease 1 (APE1) prevented TNR expansions via its 3'-5' exonuclease activity and stimulatory effect on DNA ligation during BER in a hairpin loop...
July 13, 2015: Nucleic Acids Research
https://www.readbyqxmd.com/read/23906703/is-radiographic-measurement-of-distal-femoral-torsion-reliable
#9
COMPARATIVE STUDY
T Viel, C Casin, F Ducellier, V Steiger, N Bigorre, P Bizot
BACKGROUND: Distal femur torsion (DFT) is a crucial parameter in knee replacement surgery. The reference standard for measuring DFT is posterior condylar angle (PCA) measurement using computed tomography (CT). The objective of this study was to assess the feasibility and reliability of a radiographic PCA measurement method. MATERIALS AND METHODS: We studied 125 osteoarthritic knees in 79 patients (42 women and 37 men) with a mean age of 71.6 ± 8.8 years (range 47 to 86 years); 32 knees were aligned, 85 in varus, and eight in valgus...
September 2013: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/23623316/clinical-and-technical-factors-influencing-outcomes-of-unicompartmental-knee-arthroplasty-retrospective-multicentre-study-of-944-knees
#10
A Sébilo, C Casin, B Lebel, J-L Rouvillain, S Chapuis, P Bonnevialle
INTRODUCTION: Unicompartmental knee arthroplasty (UKA) is reserved for osteoarthritis confined to a single femoro-tibial compartment with an intact anterior cruciate ligament. UKA remains controversial. The objective of this retrospective multicentre study in a large sample was to assess the influence of age, sex, body mass index (BMI), patellofemoral involvement, and implant design on functional outcomes and prosthesis survival rates. MATERIAL AND METHODS: Nine hundred and forty-four patients who underwent UKA at centres located in western France between 1988 and 2008 were re-evaluated...
June 2013: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/23403647/a-promising-yellow-phosphor-of-ce3-li-doped-casin-2-2%C3%AE-3-o%C3%AE-for-pc-leds
#11
Xiao-Ming Wang, Xue Zhang, Shi Ye, Xi-Ping Jing
Single-phased orthorhombic nitridosilicate CaSiN2 powders were prepared at 1550 °C in a N2/H2 (6%) atmosphere by solid-state reaction using Ca3N2 and Si3N4 as the starting materials. The crystal structure refinements were carried out by the Rietveld method based on the X-ray and neutron powder diffraction data collected at 297 K, respectively. The CaSiN2 phase crystallizes in an orthorhombic unit cell with the space group Pbca (No. 61) and cell parameters a = 5.1334(3) Å, b = 10.3090(5) Å, c = 14.5756(5) Å, Z = 16 (XRD data)...
April 14, 2013: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/23157279/synthesis-and-crystal-structure-of-cubic-ca16si17n34
#12
Sandra M Hick, Mattheu I Miller, Richard B Kaner, Richard G Blair
Since the late 1960s, the exact structure of cubic calcium silicon nitride has been a source of debate. This paper offers evidence that the cubic phase CaSiN(2) described in the literature is actually Ca(16)Si(17)N(34). Presented here is a method for synthesizing single crystals of cubic-calcium silicon nitride from calcium nitride and elemental silicon under flowing nitrogen at 1500 °C. The colorless millimeter-sized crystals of Ca(16)Si(17)N(34) with a refractive index (n(25)) = 1.590 were found to be cubic (a = 14...
December 3, 2012: Inorganic Chemistry
https://www.readbyqxmd.com/read/22779760/first-evidence-of-bacterial-biofilms-in-the-anaerobe-part-of-scalp-hair-follicles-a-pilot-comparative-study-in-folliculitis-decalvans
#13
COMPARATIVE STUDY
B Matard, T Meylheuc, R Briandet, I Casin, P Assouly, B Cavelier-balloy, P Reygagne
BACKGROUND: The cause of folliculitis decalvans (FD) remains unknown. We hypothesized that a bacterial biofilm could be involved in its pathogenesis. OBJECTIVE: To assess the presence or not of a bacterial biofilm in the hair roots of the scalp in FD. PATIENTS AND METHODS: Hairs plucked from four patients and three controls were examined by field emission scanning electron microscopy (FESEM) and confocal laser scanning microscopy (CLSM). RESULTS: Bacterial communities organized as biofilms were observed both by FESEM and CLSM in the under infundibular part of hair follicles in all patients and in two of the three controls...
July 2013: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/22652981/absence-of-community-acquired-methicillin-resistant-staphylococcus-aureus-carriage-or-infection-in-a-cohort-of-300-men-who-have-sex-with-men-attending-an-inner-city-sexually-transmitted-disease-clinic-in-paris-france
#14
Sébastien Fouéré, Pierre-Luc Dion, Isabelle Casin, Myriem Zouakh-Agsous, Philippe Bonhomme, Pascal Del-Giudice, Michel Janier, Olivier Chosidow
BACKGROUND: Community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) skin and soft-tissue infections are a major cause for concern. Communities of men who have sex with men (MSM) in the USA being prone to caMRSA epidemics, we investigated whether MSM attending our sexually transmitted disease clinic were carriers of or infected by caMRSA. METHODS: 300 MSM were prospectively recruited, interrogated and examined. Swabs of their nares and of their pubic and perineal skin were taken and cultured...
2012: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/22387347/clostridium-difficile-infection-after-allogeneic-hematopoietic-stem-cell-transplantation-incidence-risk-factors-and-outcome
#15
Lise Willems, Raphaël Porcher, Matthieu Lafaurie, Isabelle Casin, Marie Robin, Aliénor Xhaard, Anna Lisa Andreoli, Paula Rodriguez-Otero, Nathalie Dhedin, Gérard Socié, Patricia Ribaud, Régis Peffault de Latour
Clostridium difficile (C. difficile) infection was observed in 13% of recipients after hematopoietic stem cell transplantation (HSCT), mainly in the first month posttransplantation. Risk factors were cord blood as the source of stem cells, acute graft-versus-host disease (GVHD), and total body irradiation (TBI). No association was found with an increased risk of mortality. The purpose of this study was to evaluate the incidence, risk factors, and outcome of C. difficile infection (CDI) after HSCT. We conducted a single-center, retrospective, cohort study on all patients who received an allogeneic HSCT from January 2004 to December 2007...
August 2012: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/21828492/high-pressure-phases-and-transitions-of-the-layered-alkaline-earth-nitridosilicates-srsin-2-and-basin-2
#16
S Rebecca Römer, Peter Kroll, Wolfgang Schnick
We investigate the high-pressure phase diagram of SrSiN(2) and BaSiN(2) with density-functional calculation. Searching a manifold of possible candidate structures, we propose new structural modifications of SrSiN(2) and BaSiN(2) attainable in high-pressure experiments. The monoclinic ground state of SrSiN(2) transforms at 3 GPa into an orthorhombic BaSiN(2) type. At 14 GPa a CaSiN(2)-type structure becomes the most stable configuration of SrSiN(2). A hitherto unknown Pbcm modification is adopted at 85 GPa and, finally, at 131 GPa a LiFeO(2)-type structure...
July 8, 2009: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/21828491/a-density-functional-study-of-the-high-pressure-chemistry-of-msin-2-m-be-mg-ca-prediction-of-high-pressure-phases-and-examination-of-pressure-induced-decomposition
#17
S Rebecca Römer, Peter Kroll, Wolfgang Schnick
Normal pressure modifications and tentative high-pressure phases of the nitridosilicates MSiN(2) with M =  Be, Mg, or Ca have been thoroughly studied by density functional methods. At ambient pressure, BeSiN(2) and MgSiN(2) exhibit an ordered wurtzite variant derived from idealized filled β-cristobalite by a C1-type distortion. At ambient pressure, the structure of CaSiN(2) can also be derived from idealized filled β-cristobalite by a different type of distortion (D1-type). Energy-volume calculations for all three compounds reveal transition into an NaCl superstructure under pressure, affording sixfold coordination for Si...
July 8, 2009: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/21783081/a-novel-pyrolytic-carbon-implant-for-hallux-rigidus-a-cadaveric-study
#18
Thomas Apard, Charles Casin, Elie Moubarak, Pascal Bizot
BACKGROUND: The aim of this cadaveric study was to assess the technical feasibility of inserting a novel interpositional pyrolytic carbon coated implant in the first MTP joint, determine the best surgical procedure for the implantation, and evaluate the dynamic behavior of the joint after surgery. METHODS: The marble implant was inserted in the first metatarsophalangeal joint of five pairs of cadaveric feet using two different surgical approaches, dorsal and medial, for each pair...
September 2011: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/19946668/valgus-and-flexion-deformity-after-reconstruction-of-the-anterior-cruciate-ligament-in-a-skeletally-immature-patient
#19
Henri Emile Robert, Charles Casin
Anterior cruciate ligament reconstruction in children with open physes is still a topic of debate. We report a unique case of growth disturbance in valgus and flexion of the distal femoral epiphysis, after an ACL reconstruction in a 14.5-year-old boy. The Clocheville technique using the patellar tendon was performed. The femoral tunnel and tibial groove were both positioned above the growth plates. Eighteen months after ACL reconstruction, the patient had to be re-operated on for a valgus and flexion deformity of the femoral epiphysis...
October 2010: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/19910862/etiology-of-genital-ulcer-disease-a-prospective-study-of-278-cases-seen-in-an-std-clinic-in-paris
#20
Emilie Hope-Rapp, Vassili Anyfantakis, Sebastien Fouéré, Philippe Bonhomme, Jean B Louison, Thibault Tandeau de Marsac, Benedicte Chaine, Pascale Vallee, Isabelle Casin, Catherine Scieux, François Lassau, Michel Janier
OBJECTIVES: The goal of this study was to identify the causes and factors associated with genital ulcer disease (GUD) among patients attending a sexually transmitted disease (STD) clinic in Paris. METHODS: This study was a prospective investigation of GUD cases. Data were collected from 1995 to 2005. In each case, a Dark Field Examination (DFE), Gram stain, inoculation onto Thayer Martin agar, Columbia agar and chocolate agar with 1% isovitalex and 20% fetal calf serum, PCR Chlamydia trachomatis (Amplicor Roche), culture for herpes simplex virus (HSV) on MRC 5 cells and PCR HSV (Argene Biosoft) were obtained from the ulceration...
March 2010: Sexually Transmitted Diseases
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