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Hanna Leins, Medhanie Mulaw, Karina Eiwen, Vadim Sakk, Ying Liang, Michael Denkinger, Hartmut Geiger, Reinhold Schirmbeck
Aging-associated remodeling of the immune system impairs its functional integrity and contributes to increased morbidity and mortality in the elderly. Aging of hematopoietic stem cells (HSCs), from which all cells of the adaptive immune system ultimately originate, might play a crucial role in the remodeling of the aged immune system. We recently reported that aging of HSCs is, in part, driven by elevated activity of the small RhoGTPase Cdc42 and that aged HSCs can be rejuvenated in vitro by inhibition of the elevated Cdc42 activity in aged HSCs with the pharmacological compound CASIN...
June 11, 2018: Blood
Bauyrzhan Umbayev, Abdul-Razak Masoud, Andrey Tsoy, Dauren Alimbetov, Farkhad Olzhayev, Alla Shramko, Aiym Kaiyrlykyzy, Yuliya Safarova, Terence Davis, Sholpan Askarova
Mesenchymal stem cells (MSCs) represent a promising cell source for cellular therapy and tissue engineering and are currently being tested in a number of clinical trials for various diseases. However, like other somatic cells, MSCs age, and this senescence is accompanied by a progressive decline in stem cell function. Several lines of evidence suggest a role for the Rho family GTPase Cdc42 activity in cellular senescence processes. In the present study, we have examined aging-associated Cdc42 activity in rat adipose-derived mesenchymal stem cells (ADMSCs) and the consequences of pharmacological inhibition of Cdc42 in ADMSCs from aged rats...
May 26, 2018: Biogerontology
Qin Shao, Kevin M Casin, Nathan Mackowski, Elizabeth Murphy, Charles Steenbergen, Mark J Kohr
Nitric oxide (NO) plays an important role in cardioprotection, and recent work from our group and others has implicated protein S-nitrosylation (SNO) as a critical component of NO-mediated protection in different models, including ischemic pre- and post-conditioning and sex-dependent cardioprotection. However, studies have yet to examine whether protein SNO levels are similarly increased with pharmacologic preconditioning in male and female hearts, and whether an increase in protein SNO levels, which is protective in male hearts, is sufficient to increase baseline protection in female hearts...
2017: PloS One
Joy Seanehia, Carole Treibich, Christine Holmberg, Jacqueline Müller-Nordhorn, Valerie Casin, Jocelyn Raude, Judith E Mueller
BACKGROUND: Several concepts are available to explain vaccine decision making by individual and inter-individual factors, including risk perception, social conformism and altruism. However, only a few studies have quantified the weight of these determinants in vaccine acceptance. Using a conjoint analysis tool, we aimed at eliciting preferences in a student population regarding vaccination against a rare, severe and rapidly evolving hypothetical disease, similar to meningococcal serogroup C meningitis or measles...
May 9, 2017: Vaccine
Yongru Xu, Yingzi Qi, Jing Luo, Jing Yang, Qi Xie, Chen Deng, Na Su, Wei Wei, Deshun Shi, Feng Xu, Xiangping Li, Ping Xu
Chronic hepatitis B virus (HBV) infection has been considered as the major cause of hepatocellular carcinoma (HCC). Hepatitis B virus X protein (HBx) has been reported to be oncogenic. The underlying mechanisms of HBV-related HCC are not fully understood, and the role played by the HBx protein in HBV induced carcinogenesis remains controversial. CDC42, a member of the Rho GTPase family, has been reported to be overexpressed in several different cancers, including HBV-related HCC. However, the specific role of CDC42 in HCC development remains unclear...
March 8, 2017: International Journal of Molecular Sciences
A Palazzo, O Bluteau, K Messaoudi, F Marangoni, Y Chang, S Souquere, G Pierron, V Lapierre, Y Zheng, W Vainchenker, H Raslova, N Debili
Essentials The role of the cytoskeleton during megakaryocyte differentiation was examined. Human megakaryocytes are derived from in vitro cultured CD34+ cells. Cell division control protein 42 (CDC42) positively regulates proplatelet formation (PPF). Neural Wiskott-Aldrich syndrome protein, the main effector of CDC42 with Src positively regulates PPF. SUMMARY: Background Cytoskeletal rearrangements are essential for platelet release. The RHO small GTPase family, as regulators of the actin cytoskeleton, play an important role in proplatelet formation (PPF)...
December 2016: Journal of Thrombosis and Haemostasis: JTH
Zhihai Zhao, Song Hui Tan, Hiroaki Machiyama, Keiko Kawauchi, Keigo Araki, Hiroaki Hirata, Yasuhiro Sawada
Cell migration is a highly dynamic process that plays pivotal roles in both physiological and pathological processes. We have previously reported that p130Cas supports cell migration through the binding to Src as well as phosphorylation-dependent association with actin retrograde flow at focal adhesions. However, it remains elusive how phosphorylated Cas interacts with actin cytoskeletons. We observe that the actin-binding protein, tensin 1, co-localizes with Cas, but not with its phosphorylation-defective mutant, at focal adhesions in leading regions of migrating cells...
2016: Biology Open
S Yahia, S Benomar, F Dehiba, A Allaoui, M J Rodriguez-Yoldi, J Osada, A Boualga
OBJECTIFS: Le but de ce travail est d'étudier l'effet des protéines de pois chiche hydrolysées (HPC) sur la composition lipidique et la peroxydation lipidique des HDL chez les rats hypercholestérolémiques. MéTHODES: Des rats mâles Wistar (220±10g) consommant un régime à 20 % de caséine avec 1 % de cholestérol et 0,5 % d'acide cholique sont divisés en trois lot de 6 rats reçoivent pendant 30 jours par gavage 1g/kg une solution de HPC préparées à DH = 8 % (HPC8) ou DH = 17 % (HPC17), le troisième reçoit de l'eau et constitue le groupe control (GC)...
December 2015: Annales de Cardiologie et D'angéiologie
A Benyahia Mostefaoui, M Y Lamri Senhadji
OBJECTIFS: Voir si les protéines de sardine comparées à la caséine, combinées aux lipides du lait ou à l'huile de sardine peuvent moduler différemment la dyslipidémie et atténuer le stress oxydatif induit par un régime athérogène. MéTHODES: Des rats mâles (n=24) pesant 200±10g sont divisés en 4 groupes et reçoivent pendant 28 jours, 20 % de protéines de sardine ou de caséine combinées à 5 % d'huile de sardine (PsHS et casHS) ou de lipides du lait (PsLL et casLL). Les 4 régimes sont supplémentés avec 1 % de cholestérol et 0,5 % d'acide cholique...
December 2015: Annales de Cardiologie et D'angéiologie
S Benomar, S Yahya, F Dehiba, A Allaoui, M J Rodriguez-Yoldi, J Osada, A Boualga
OBJECTIFS: Il est bien établi que les lipoprotéines jouent un rôle important dans le processus de l'athérosclérose et particulièrement les HDL qui agissent contre cette pathologie vasculaire. Le but de ce travail est d'étudier si les hydrolysats de protéines de sardine (HPS) ou de bogue (HPB) protègent l'aorte en améliorant les propriétés antioxydantes des HDL chez le rat hypercholesterolemique. MéTHODES: 18 rats mâles Wistar (210±10 g), répartis en trois groupes de six rats chacun, consommant durant 28 jours un régime à 20 % de caséine supplémenté avec 1 % de cholestérol alimentaire et 0,5 % d'acide cholique...
December 2015: Annales de Cardiologie et D'angéiologie
S Bouderbala, M Al-Hiti Kn, M Bouchenak
OBJECTIFS: L'effet des grignons d'olives (GO) après extraction d'huile est étudié sur l'activité antioxydante tissulaire chez des rats consommant un régime enrichi en cholestérol. MéTHODES: Des rats mâles de souche Wistar pesant 80±5g (n=24) sont soumis pendant 28 jours à un régime contenant 20 % de caséine + 1 % de cholestérol (HC) supplémenté ou non avec les grignons d'olives à 2,5 %, 5 % et 7,5 % (HC-GO2,5, HC-GO5 et HC-GO7,5, respectivement). RéSULTATS: Les rats hypercholestérolémiques soumis aux régimes supplémentés en GO sont comparés au groupe HC...
December 2015: Annales de Cardiologie et D'angéiologie
Qin Shao, Jonathan Fallica, Kevin M Casin, Elizabeth Murphy, Charles Steenbergen, Mark J Kohr
Premenopausal women exhibit endogenous cardioprotective signaling mechanisms that are thought to result from the beneficial effects of estrogen, which we have shown to increase protein S-nitrosylation in the heart. S-nitrosylation is a labile protein modification that increases with a number of different forms of cardioprotection, including ischemic preconditioning. Herein, we sought to identify a potential role for protein S-nitrosylation in sex-dependent cardioprotection. We utilized a Langendorff-perfused mouse heart model of ischemia-reperfusion injury with male and female hearts, and S-nitrosylation-resin-assisted capture with liquid chromatography tandem mass spectrometry to identify S-nitrosylated proteins and modification sites...
February 15, 2016: American Journal of Physiology. Heart and Circulatory Physiology
Jill M Beaver, Yanhao Lai, Meng Xu, Astrid H Casin, Eduardo E Laverde, Yuan Liu
Base excision repair (BER) of an oxidized base within a trinucleotide repeat (TNR) tract can lead to TNR expansions that are associated with over 40 human neurodegenerative diseases. This occurs as a result of DNA secondary structures such as hairpins formed during repair. We have previously shown that BER in a TNR hairpin loop can lead to removal of the hairpin, attenuating or preventing TNR expansions. Here, we further provide the first evidence that AP endonuclease 1 (APE1) prevented TNR expansions via its 3'-5' exonuclease activity and stimulatory effect on DNA ligation during BER in a hairpin loop...
July 13, 2015: Nucleic Acids Research
T Viel, C Casin, F Ducellier, V Steiger, N Bigorre, P Bizot
BACKGROUND: Distal femur torsion (DFT) is a crucial parameter in knee replacement surgery. The reference standard for measuring DFT is posterior condylar angle (PCA) measurement using computed tomography (CT). The objective of this study was to assess the feasibility and reliability of a radiographic PCA measurement method. MATERIALS AND METHODS: We studied 125 osteoarthritic knees in 79 patients (42 women and 37 men) with a mean age of 71.6 ± 8.8 years (range 47 to 86 years); 32 knees were aligned, 85 in varus, and eight in valgus...
September 2013: Orthopaedics & Traumatology, Surgery & Research: OTSR
A Sébilo, C Casin, B Lebel, J-L Rouvillain, S Chapuis, P Bonnevialle
INTRODUCTION: Unicompartmental knee arthroplasty (UKA) is reserved for osteoarthritis confined to a single femoro-tibial compartment with an intact anterior cruciate ligament. UKA remains controversial. The objective of this retrospective multicentre study in a large sample was to assess the influence of age, sex, body mass index (BMI), patellofemoral involvement, and implant design on functional outcomes and prosthesis survival rates. MATERIAL AND METHODS: Nine hundred and forty-four patients who underwent UKA at centres located in western France between 1988 and 2008 were re-evaluated...
June 2013: Orthopaedics & Traumatology, Surgery & Research: OTSR
Xiao-Ming Wang, Xue Zhang, Shi Ye, Xi-Ping Jing
Single-phased orthorhombic nitridosilicate CaSiN2 powders were prepared at 1550 °C in a N2/H2 (6%) atmosphere by solid-state reaction using Ca3N2 and Si3N4 as the starting materials. The crystal structure refinements were carried out by the Rietveld method based on the X-ray and neutron powder diffraction data collected at 297 K, respectively. The CaSiN2 phase crystallizes in an orthorhombic unit cell with the space group Pbca (No. 61) and cell parameters a = 5.1334(3) Å, b = 10.3090(5) Å, c = 14.5756(5) Å, Z = 16 (XRD data)...
April 14, 2013: Dalton Transactions: An International Journal of Inorganic Chemistry
Sandra M Hick, Mattheu I Miller, Richard B Kaner, Richard G Blair
Since the late 1960s, the exact structure of cubic calcium silicon nitride has been a source of debate. This paper offers evidence that the cubic phase CaSiN(2) described in the literature is actually Ca(16)Si(17)N(34). Presented here is a method for synthesizing single crystals of cubic-calcium silicon nitride from calcium nitride and elemental silicon under flowing nitrogen at 1500 °C. The colorless millimeter-sized crystals of Ca(16)Si(17)N(34) with a refractive index (n(25)) = 1.590 were found to be cubic (a = 14...
December 3, 2012: Inorganic Chemistry
B Matard, T Meylheuc, R Briandet, I Casin, P Assouly, B Cavelier-balloy, P Reygagne
BACKGROUND: The cause of folliculitis decalvans (FD) remains unknown. We hypothesized that a bacterial biofilm could be involved in its pathogenesis. OBJECTIVE: To assess the presence or not of a bacterial biofilm in the hair roots of the scalp in FD. PATIENTS AND METHODS: Hairs plucked from four patients and three controls were examined by field emission scanning electron microscopy (FESEM) and confocal laser scanning microscopy (CLSM). RESULTS: Bacterial communities organized as biofilms were observed both by FESEM and CLSM in the under infundibular part of hair follicles in all patients and in two of the three controls...
July 2013: Journal of the European Academy of Dermatology and Venereology: JEADV
Sébastien Fouéré, Pierre-Luc Dion, Isabelle Casin, Myriem Zouakh-Agsous, Philippe Bonhomme, Pascal Del-Giudice, Michel Janier, Olivier Chosidow
BACKGROUND: Community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) skin and soft-tissue infections are a major cause for concern. Communities of men who have sex with men (MSM) in the USA being prone to caMRSA epidemics, we investigated whether MSM attending our sexually transmitted disease clinic were carriers of or infected by caMRSA. METHODS: 300 MSM were prospectively recruited, interrogated and examined. Swabs of their nares and of their pubic and perineal skin were taken and cultured...
2012: Dermatology: International Journal for Clinical and Investigative Dermatology
Lise Willems, Raphaël Porcher, Matthieu Lafaurie, Isabelle Casin, Marie Robin, Aliénor Xhaard, Anna Lisa Andreoli, Paula Rodriguez-Otero, Nathalie Dhedin, Gérard Socié, Patricia Ribaud, Régis Peffault de Latour
Clostridium difficile (C. difficile) infection was observed in 13% of recipients after hematopoietic stem cell transplantation (HSCT), mainly in the first month posttransplantation. Risk factors were cord blood as the source of stem cells, acute graft-versus-host disease (GVHD), and total body irradiation (TBI). No association was found with an increased risk of mortality. The purpose of this study was to evaluate the incidence, risk factors, and outcome of C. difficile infection (CDI) after HSCT. We conducted a single-center, retrospective, cohort study on all patients who received an allogeneic HSCT from January 2004 to December 2007...
August 2012: Biology of Blood and Marrow Transplantation
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