keyword
MENU ▼
Read by QxMD icon Read
search

Transplant animal needs

keyword
https://www.readbyqxmd.com/read/29149983/a-novel-model-of-mouse-to-rat-kidney-xenotransplantation
#1
N Özçay, H Özdemir
Transplantation animal models require 2 animals for each experiment, 1 as a donor and 1 as a recipient. At the present time, developing microsurgical instruments and refining surgical techniques should allow us to reduce the number of animal used for transplantation research. In this study, we aimed to harvest 2 kidneys from 1 donor to be able to minimize the number of animals needed for transplantation studies. For this purpose, we developed a kidney xenotransplantation model from mouse to rat, in which only 1 animal was used as the donor for 2 kidney recipients...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29141010/multiple-effects-of-toxins-isolated-from-crotalus-durissus-terrificus-on-the-hepatitis-c-virus-life-cycle
#2
Jacqueline Farinha Shimizu, Carina Machado Pereira, Cintia Bittar, Mariana Nogueira Batista, Guilherme Rodrigues Fernandes Campos, Suely da Silva, Adélia Cristina Oliveira Cintra, Carsten Zothner, Mark Harris, Suely Vilela Sampaio, Victor Hugo Aquino, Paula Rahal, Ana Carolina Gomes Jardim
Hepatitis C virus (HCV) is one of the main causes of liver disease and transplantation worldwide. Current therapy is expensive, presents additional side effects and viral resistance has been described. Therefore, studies for developing more efficient antivirals against HCV are needed. Compounds isolated from animal venoms have shown antiviral activity against some viruses such as Dengue virus, Yellow fever virus and Measles virus. In this study, we evaluated the effect of the complex crotoxin (CX) and its subunits crotapotin (CP) and phospholipase A2 (PLA2-CB) isolated from the venom of Crotalus durissus terrificus on HCV life cycle...
2017: PloS One
https://www.readbyqxmd.com/read/29138636/stem-cell-tracking-technologies-for-neurological-regenerative-medicine-purposes
#3
REVIEW
Yongtao Zheng, Jiongwei Huang, Tongming Zhu, Ronggang Li, Zhifu Wang, Fukai Ma, Jianhong Zhu
The growing field of stem cell therapy is moving toward clinical trials in a variety of applications, particularly for neurological diseases. However, this translation of cell therapies into humans has prompted a need to create innovative and breakthrough methods for stem cell tracing, to explore the migration routes and its reciprocity with microenvironment targets in the body, to monitor and track the outcome after stem cell transplantation therapy, and to track the distribution and cell viability of transplanted cells noninvasively and longitudinally...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29130076/crispr-correction-of-a-homozygous-low-density-lipoprotein-receptor-mutation-in-familial-hypercholesterolemia-induced-pluripotent-stem-cells
#4
Linda Omer, Elizabeth A Hudson, Shirong Zheng, James B Hoying, Yuan Shan, Nolan L Boyd
Familial hypercholesterolemia (FH) is a hereditary disease primarily due to mutations in the low-density lipoprotein receptor (LDLR) that lead to elevated cholesterol and premature development of cardiovascular disease. Homozygous FH patients (HoFH) with two dysfunctional LDLR alleles are not as successfully treated with standard hypercholesterol therapies, and more aggressive therapeutic approaches to control cholesterol levels must be considered. Liver transplant can resolve HoFH, and hepatocyte transplantation has shown promising results in animals and humans...
November 2017: Hepatol Commun
https://www.readbyqxmd.com/read/29109530/a-clinical-scale-bioartificial-liver-developed-for-gmp-improved-clinical-parameters-of-liver-function-in-porcine-liver-failure
#5
Clare Selden, James Bundy, Eloy Erro, Eva Puschmann, Malcolm Miller, Delawir Kahn, Humphrey Hodgson, Barry Fuller, Jordi Gonzalez-Molina, Aurelie Le Lay, Stephanie Gibbons, Sherri Chalmers, Sunil Modi, Amy Thomas, Peter Kilbride, Agnes Isaacs, Richard Ginsburg, Helen Ilsley, David Thomson, Galya Chinnery, Ncedile Mankahla, Lizel Loo, C Wendy Spearman
Liver failure, whether arising directly from acute liver failure or from decompensated chronic liver disease is an increasing problem worldwide and results in many deaths. In the UK only 10% of individuals requiring a liver transplant receive one. Thus the need for alternative treatments is paramount. A BioArtificial Liver machine could temporarily replace the functions of the liver, buying time for the patient's liver to repair and regenerate. We have designed, implemented and tested a clinical-scale BioArtificial Liver machine containing a biomass derived from a hepatoblastoma cell-line cultured as three dimensional organoids, using a fluidised bed bioreactor, together with single-use bioprocessing equipment, with complete control of nutrient provision with feedback BioXpert recipe processes, and yielding good phenotypic liver functions...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29105567/in-vivo-survival-of-human-endometrial-mesenchymal-stem-cells-transplanted-under-the-kidney-capsule-of-immunocompromised-mice
#6
Shanti Gurung, James A Deane, Saeedeh Darzi, Jerome A Werkmeister, Caroline E Gargett
Human endometrial mesenchymal stem cells (eMSCs) are a well-characterised adult stem cell type with potential for use in regenerative medicine or cell-therapy. As a proof of principle, we demonstrated that eMSCs promoted wound healing by reducing the inflammatory response through a paracrine action in a subcutaneous rat model of wound repair. However, an efficient protocol for culturing eMSCs in the undifferentiated state and a reliable method of labelling them for cell tracking were lacking. Here, we investigated the use of a lentiviral vector containing the mCherry fluorescent reporter gene to transduce and label eMSCs following in vitro culturing in A83-01-containing medium, and different methods of tracing the labelled cells following transplantation under the kidney capsule of immunocompromised NSG mice...
November 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29033833/cell-based-therapies-for-tissue-fibrosis
#7
REVIEW
Rebecca Lim, Sharon D Ricardo, William Sievert
The development of tissue fibrosis in the context of a wound-healing response to injury is common to many chronic diseases. Unregulated or persistent fibrogenesis may lead to structural and functional changes in organs that increase the risk of significant morbidity and mortality. We will explore the natural history, epidemiology, and pathogenesis of fibrotic disease affecting the lungs, kidneys, and liver as dysfunction of these organs is responsible for a substantial proportion of global mortality. For many patients with end-stage disease, organ transplantation is the only effective therapy to prolong life...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29030945/adenine-induced-chronic-kidney-disease-in-rats
#8
REVIEW
Vishal Diwan, Lindsay Brown, Glenda C Gobe
Many animal models have been developed to study the causes and treatments of chronic kidney disease (CKD) in humans, an insidious disease resulting from kidney injury and characterised by persistent functional decline for more than 3 months, with or without evidence of structural deficit. The eventual outcome of CKD may be end-stage kidney disease (ESKD), where patients need dialysis or transplantation to survive. Cardiovascular disease is accelerated in patients with CKD and contributes to increased mortality, with the relationship between CKD and cardiovascular disease being bi-directional...
October 14, 2017: Nephrology
https://www.readbyqxmd.com/read/29017955/triggering-receptor-expressed-on-myeloid-cells-2-expression-tracks-with-m2-like-macrophage-activity-and-disease-severity-in%C3%A2-copd
#9
Derek E Byers, Kangyun Wu, Geoffrey Dang-Vu, Xiaohua Jin, Eugene Agapov, Xiaofeng Zhang, John T Battaile, Kenneth Schechtman, Roger Yusen, Richard A Pierce, Michael J Holtzman
BACKGROUND: Cell and animal models show a key role for Triggering Receptor Expressed on Myeloid Cells (TREM)-2 in chronic airway disease after viral infection, but comparable evidence in humans still needs to be established. METHODS: Lung tissue samples were obtained from lung transplant recipients with Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage IV COPD (n = 16), nontransplantable donor lung tissues (n = 10), and resected lung tissues from patients at risk or with GOLD stage I through IV (n = 72) and were assessed for TREM-2 and TREM-1 messenger RNA (mRNA), protein expression, and other markers of a type 2 immune response...
October 7, 2017: Chest
https://www.readbyqxmd.com/read/28987031/using-minimalist-self-assembling-peptides-as-hierarchical-scaffolds-to-stabilize-growth-factors-and-promote-stem-cell-integration-in-the-injured-brain
#10
A L Rodriguez, K F Bruggeman, Y Wang, T Y Wang, R J Williams, C L Parish, D R Nisbet
Neurotrophic growth factors are effective in slowing progressive degeneration and/or promoting neural repair through the support of residual host and/or transplanted neurons. However, limitations including short half-life and enzyme susceptibility of growth factors highlight the need for alternative strategies to prolong localised delivery at a site of injury. Here, we establish the utility of minimalist N-fluorenylmethyloxycarbonyl (Fmoc) self-assembling peptides (SAPs) as growth factor delivery vehicle, targeted at supporting neural transplants in an animal model of Parkinson's disease...
October 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28985317/manipulation-of-gut-microbiota-reveals-shifting-community-structure-shaped-by-host-developmental-windows-in-amphibian-larvae
#11
Robin W Warne, Lucas Kirschman, Lydia Zeglin
Exploration of the importance of developmental windows for microbial colonization in diverse animal taxa, and tests of how these shape both animal microbiomes as well as host phenotypes promise to shed needed light on host-microbe interactions. The aims of this study were to explore how gut microbiota diversity of larval amphibians varies among species and across ontogeny, and to test if manipulation of gut colonization can reveal how microbiomes develop. We found that gut microbiomes differ among species and change across larval ontogeny, with distinctive differences between larvae, metamorphic animals, and juvenile frogs...
July 26, 2017: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/28984641/prospects-for-adoptive-t-cell-therapy-for-invasive-fungal-disease
#12
Gloria Castellano-Gonzalez, Leighton E Clancy, David Gottlieb
PURPOSE OF REVIEW: Invasive fungal disease (IFD) is a cause of morbidity and mortality in allogeneic hematopoietic stem cell transplant (HSCT) recipients. As more potent broad-spectrum antifungal agents are used in prophylaxis, drug resistance and less common fungal species have increased in frequency. Here we review current treatments available for IFD and examine the potential for adoptive T-cell treatment to enhance current therapeutic choices in IFD. RECENT FINDINGS: There is growing evidence supporting the role of T cells as well as phagocytes in antifungal immunity...
October 3, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28962587/comparative-characteristics-of-laryngeal-resident-mesenchymal-stromal-cell-populations-isolated-from-distinct-sites-in-the-rat-larynx
#13
Songyi Lee, Yeseulmi Kim, Hyun-Soo Shin, Jae-Yol Lim
BACKGROUND: Although tissue-resident mesenchymal stromal cells (MSCs) in the larynx have been described, their distinct characteristics and roles have not been thoroughly explored. Therefore, we investigated stem cell characteristics and regenerative potentials of single clonal populations isolated from rat epiglottic mucosa (EM), lamina propria (LP), and macula flava (MF) to determine whether they comprised laryngeal tissue-resident stem cells. METHODS: Single clonal laryngeal cells were isolated following microdissection of the EM, LP, and MF from the rat larynx...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28936741/exosomes-derived-from-embryonic-stem-cells-as-potential-treatment-for-cardiovascular-diseases
#14
Yao-Hua Song, Lianbo Shao, Yu Zhang, Jin Zhou, Bin Liu, Xiangbin Pan, Yong-Jian Geng, Xi-Yong Yu, Yangxin Li
Cardiovascular diseases resulting from ischemic heart diseases remain to be the main causes of heart failure and death despite significant advances in medical treatment. The development of new therapies for heart failure is thus required to improve the outcome in these patients, and this has led to the development of cell-based therapies. Animal studies showed interesting results using various cell types. Some stem cell based therapies have been tested in clinical trials. Although the results were encouraging, challenges remain...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28931067/in-vivo-imaging-system-for-explants-analysis-a-new-approach-for-assessment-of-cell-transplantation-effects-in-large-animal-models
#15
Weronika Zarychta-Wiśniewska, Anna Burdzinska, Radosław Zagozdzon, Bartosz Dybowski, Marta Butrym, Zdzisław Gajewski, Leszek Paczek
INTRODUCTION: Despite spectacular progress in cellular transplantology, there are still many concerns about the fate of transplanted cells. More preclinical studies are needed, especially on large animal models, to bridge the translational gap between basic research and the clinic. Herein, we propose a novel approach in analysis of cell transplantation effects in large animals explants using in vivo imaging system (IVIS®) or similar equipment. MATERIAL AND METHODS: In the in vitro experiment cells labeled with fluorescent membrane dyes: DID (far red) or PKH26 (orange) were visualized with IVIS®...
2017: PloS One
https://www.readbyqxmd.com/read/28928775/assessment-of-safety-and-functional-efficacy-of-stem-cell-based-therapeutic-approaches-using-retinal-degenerative-animal-models
#16
REVIEW
Tai-Chi Lin, Magdalene J Seiler, Danhong Zhu, Paulo Falabella, David R Hinton, Dennis O Clegg, Mark S Humayun, Biju B Thomas
Dysfunction and death of retinal pigment epithelium (RPE) and or photoreceptors can lead to irreversible vision loss. The eye represents an ideal microenvironment for stem cell-based therapy. It is considered an "immune privileged" site, and the number of cells needed for therapy is relatively low for the area of focused vision (macula). Further, surgical placement of stem cell-derived grafts (RPE, retinal progenitors, and photoreceptor precursors) into the vitreous cavity or subretinal space has been well established...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28905528/humidifier-disinfectant-associated-lung-injury-six-years-after-the-tragic-event
#17
REVIEW
Won Young Kim, Sang Bum Hong
In 2011, a cluster of peripartum patients were admitted to the intensive care unit of a tertiary hospital in Seoul with signs and symptoms of severe respiratory distress of unknown etiology. Subsequent epidemiological and animal studies suggested that humidifier disinfectant (HD) might represent the source of this pathology. Epidemiological studies, animal studies, and dose-response analysis demonstrated a strong association between HD use and lung injuries. The diagnostic criteria for HD-associated lung injury (HDALI) was defined on the basis of the clinical, pathological, and radiological attributes of the patients...
October 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28901972/normothermic-machine-perfusion-of-the-kidney
#18
Annemarie Weissenbacher, James Hunter
PURPOSE OF REVIEW: Normothermic machine perfusion (NMP) is a preservation method that is generating increasing interest. The aim of this review is to summarise the current status of NMP in regards of kidney viability assessment, reducing organ damage and improving transplant logistics. RECENT FINDINGS: The results of recent large animal experiments and clinical trials show that continuous prolonged normothermic ex-vivo kidney perfusion appears better than a brief period of NMP after static cold storage in terms of renal injury and function...
December 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28892571/regenerative-immunology-the-immunological-reaction-to-biomaterials
#19
REVIEW
Paolo Cravedi, Samira Farouk, Andrea Angeletti, Lauren Edgar, Riccardo Tamburrini, Jerome Duisit, Laura Perin, Giuseppe Orlando
Regenerative medicine promises to meet two of the most urgent needs of modern organ transplantation, namely immunosuppression-free transplantation and an inexhaustible source of organs. Ideally, bioengineered organs would be manufactured from a patient's own biomaterials-both cells and the supporting scaffolding materials in which cells would be embedded and allowed to mature to eventually regenerate the organ in question. While some groups are focusing on the feasibility of this approach, few are focusing on the immunogenicity of the scaffolds that are being developed for organ bioengineering purposes...
September 11, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28884412/mesenchymal-stem-cells-mscs-therapy-for-recovery-of-fertility-a-systematic-review
#20
REVIEW
Zahra Fazeli, Atieh Abedindo, Mir Davood Omrani, Sayyed Mohammad Hossein Ghaderian
In recent years, the mesenchymal stem cells (MSCs) have provided the new opportunities to treat different disorders including infertility. Different studies have suggested that the MSCs have ability to differentiate into germ-like cells under specific induction conditions as well as transplantation to gonadal tissues. The aim of this systematic review was to evaluate the results obtained from different studies on MSCs therapy for promoting fertility. This search was done in PubMed and Science Direct databases using key words MSCs, infertility, therapy, germ cell, azoospermia, ovarian failure and mesenchymal stem cell...
September 7, 2017: Stem Cell Reviews
keyword
keyword
98255
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"