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https://www.readbyqxmd.com/read/28926588/the-effects-of-storage-temperature-and-duration-of-blood-samples-on-dna-and-rna-qualities
#1
Lien-Hung Huang, Pei-Hsien Lin, Kuo-Wang Tsai, Liang-Jen Wang, Ying-Hsien Huang, Ho-Chang Kuo, Sung-Chou Li
DNA and RNA samples from blood are the common examination target for non-invasive physical tests and/or biomedical studies. Since high-quality DNA and RNA samples guarantee the correctness of these tests and/or studies, we investigated the effects of storage temperature and storage duration of whole blood on DNA and RNA qualities. Subjects were enrolled to donate blood samples which were stored for different durations and at different temperatures, followed by the examinations on RNA quality, qPCR, DNA quality and DNA methylation...
2017: PloS One
https://www.readbyqxmd.com/read/28926390/misconceptions-misuses-and-misinterpretations-of-p-values-and-significance-testing
#2
Joel J Gagnier, Hal Morgenstern
The interpretation and reporting of p values and significance testing in biomedical research are fraught with misconceptions and inaccuracies. Publications of peer-reviewed research in orthopaedics are not immune to such problems. The American Statistical Association (ASA) recently published an official statement on the use, misuse, and misinterpretation of statistical testing and p values in applied research. The ASA statement discussed 6 principles: (1) "P-values can indicate how incompatible the data are with a specified statistical model...
September 20, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28923420/high-doses-of-lipid-core-nanocapsules-do-not-affect-bovine-embryonic-development-in-vitro
#3
Caroline Lucas, Mariana Remião, Franciele Bruinsmann, Isadora Lopes, Morgana Borges, Ana Laura Feijó, Andrea Cristina Basso, Adriana Pohlmann, Silvia Guterres, Vinicius Campos, Fabiana Seixas, Tiago Collares
The improvement of in vitro embryo production by culture media supplementation has been a potential tool to increase blastocyst quality and development. Recently, lipid-core nanocapsules (LNC), which were developed for biomedical applications as a drug-delivery system, have demonstrated beneficial effects on in vitro embryo production studies. LNCs have a core composed of sorbitan monostearate dispersed in capric/caprylic triglyceride. Based on that, we firstly investigated if LNCs supplemented during in vitro oocyte maturation had affinity to the mineral oil placed over the top of the IVM media...
September 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28923006/repeat-a-framework-to-assess-empirical-reproducibility-in-biomedical-research
#4
Leslie D McIntosh, Anthony Juehne, Cynthia R H Vitale, Xiaoyan Liu, Rosalia Alcoser, J Christian Lukas, Bradley Evanoff
BACKGROUND: The reproducibility of research is essential to rigorous science, yet significant concerns of the reliability and verifiability of biomedical research have been recently highlighted. Ongoing efforts across several domains of science and policy are working to clarify the fundamental characteristics of reproducibility and to enhance the transparency and accessibility of research. METHODS: The aim of the proceeding work is to develop an assessment tool operationalizing key concepts of research transparency in the biomedical domain, specifically for secondary biomedical data research using electronic health record data...
September 18, 2017: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/28918110/emergent-behaviors-in-rbcs-flows-in-micro-channels-using-digital-particle-image-velocimetry
#5
F Cairone, D Ortiz, P J Cabrales, M Intaglietta, M Bucolo
The key points in the design of microfluidic Lab-On-a-Chips for blood tests are the simplicity of the microfluidic chip geometry, the portability of the monitoring system and the ease on-chip integration of the data analysis procedure. The majority of those, recently designed, have been used for blood separation, however their introduction, also, for pathological conditions diagnosis would be important in different biomedical contexts. To overcome this lack is necessary to establish the relation between the RBCs flow and blood viscosity changes in micro-vessels...
September 13, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28915042/mode-of-peg-coverage-on-carbon-nanotubes-affects-binding-of-innate-immune-protein-c1q
#6
Agathe Belime, Edmond Gravel, Sophie Brenet, Sarah Ancelet, Charlotte Caneiro, Yanxia Hou, Nicole Thielens, Eric Doris, Wai Li Ling
Surface modification of nanoparticles with poly(ethylene glycol) PEG is used in biomedicines to increase the circulation time of the particles after intravenous injection. Here, we study the interaction of PEG-covered carbon nanotubes (CNTs) with the serum complement protein C1q. Besides being the target-recognizing unit of the initiating complex for the classical pathway of complement in our innate immune system, C1q is involved in a range of important physiological processes. We modified the surface of multi-walled CNTs with covalently grafted PEG and physically adsorbed PEG...
September 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28915005/a-novel-type-of-aqueous-dispersible-ultrathin-layered-double-hydroxide-nanosheets-for-in-vivo-bioimaging-and-drug-delivery
#7
Li Yan, Mengjiao Zhou, Xiujuan Zhang, Longbiao Huang, Wei Chen, Vellaisamy A L Roy, WenJun Zhang, Xianfeng Chen
Layered double hydroxide (LDH) nanoparticles have been widely used for various biomedical applications. However, because of the difficulty of surface functionalization of LDH nanoparticles, the systemic administration of these nanomaterials for in vivo therapy remains a bottleneck. In this work, we develop a novel type of aqueous dispersible two dimensional ultrathin LDH nanosheets with a size of about 50 nm and a thickness of about 1.4 to 4 nm. We are able to covalently attach positively charged Rhodamine B fluorescent molecules to the nanosheets and the nanohybrid retains strong fluorescence in liquid and even dry powder form...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28914459/testing-a-biopsychosocial-model-of-the-basic-birth-beliefs
#8
Heidi Preis, Rony Chen, Michal Eisner, Joseph Pardo, Yoav Peled, Arnon Wiznitzer, Yael Benyamini
BACKGROUND: Women perceive what birth is even before they are pregnant for the first time. Part of this conceptualization is the basic belief about birth as a medical and natural process. These two separate beliefs are pivotal in the decision-making process about labor and birth. Adapting Engel's biopsychosocial framework, we explored the importance of a wide range of factors which may contribute to these beliefs among first-time mothers. METHOD: This observational study included 413 primiparae ≥24 weeks' gestation, recruited in medical centers and in natural birth communities in Israel...
September 15, 2017: Birth
https://www.readbyqxmd.com/read/28913652/differences-in-bone-structure-and-unloading-induced-bone-loss-between-c57bl-6n-and-c57bl-6j-mice
#9
Jeyantt S Sankaran, Manasvi Varshney, Stefan Judex
The C57BL/6 mouse, the most frequently utilized animal model in biomedical research, is in use as several substrains, all of which differ by a small array of genomic differences. Two of these substrains, C57BL/6J (B6J) and C57BL/6N (B6N), are commonly used but it is unclear how phenotypically similar or different they are. Here, we tested whether adolescent B6N mice have a bone phenotype and respond to the loss of weightbearing differently than B6J. At 9 weeks of age, normally ambulating B6N had lower trabecular bone volume fraction but greater bone formation rates and osteoblast surfaces than corresponding B6J...
September 14, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28912836/methods-for-enhancing-the-reproducibility-of-biomedical-research-findings-using-electronic-health-records
#10
REVIEW
Spiros Denaxas, Kenan Direk, Arturo Gonzalez-Izquierdo, Maria Pikoula, Aylin Cakiroglu, Jason Moore, Harry Hemingway, Liam Smeeth
BACKGROUND: The ability of external investigators to reproduce published scientific findings is critical for the evaluation and validation of biomedical research by the wider community. However, a substantial proportion of health research using electronic health records (EHR), data collected and generated during clinical care, is potentially not reproducible mainly due to the fact that the implementation details of most data preprocessing, cleaning, phenotyping and analysis approaches are not systematically made available or shared...
2017: BioData Mining
https://www.readbyqxmd.com/read/28902169/whispering-gallery-mode-resonators-for-detecting-cancer
#11
REVIEW
Weeratouch Pongruengkiat, Suejit Pechprasarn
Optical resonators are sensors well known for their high sensitivity and fast response time. These sensors have a wide range of applications, including in the biomedical fields, and cancer detection is one such promising application. Sensor diagnosis currently has many limitations, such as being expensive, highly invasive, and time-consuming. New developments are welcomed to overcome these limitations. Optical resonators have high sensitivity, which enable medical testing to detect disease in the early stage...
September 13, 2017: Sensors
https://www.readbyqxmd.com/read/28900615/exploring-genetic-numeracy-skills-in-a-sample-of-u-s-university-students
#12
Margo W Bergman, Patricia Goodson, Heather Honoré Goltz
Misconceptions concerning numerical genetic risk exist even within educated populations. To more fully characterize and understand the extent of these risk misunderstandings, which have large potential impact on clinical care, we analyzed the responses from 2,576 students enrolled at 2 Southwestern universities using the PGRID tool, a 138-item web-based survey comprising measures of understanding of genetics, genetic disease, and genetic risk. The primary purpose of this study was to characterize the intersection of risk perception and knowledge, termed genetic numeracy (GN)...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28900075/-circulating-micrornas-in-the-identification-of-biological-fluids-a-new-approach-to-standardization-of-expression-based-diagnostics
#13
A S Bavykin
Molecular profiling of normal tissues is a regular and necessary step when developing systems for expression analyses in biological samples, including diagnostic panels for various diseases and conditions. Yet there are still no rigorous criteria to allow precise typing of normal tissues. A main problem is that the methods employed in diagnostic expression testing are difficult to standardize. While various technologies, instruments, and reagents are available, universal protocols of handling biological material are lacking, thus impairing the reproducibility of data from independent studies...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28898765/development-of-quantitative-immunochromatographic-assay-for-rapid-and-sensitive-detection-of-carbohydrate-antigen-19-9-ca-19-9-in-human-plasma
#14
Kwaku Baryeh, Sunitha Takalkar, Michelle Lund, Guodong Liu
A quantitative immunochromatographic assay (QIA) was developed by using gold nanoparticle (GNP)-based lateral flow strip biosensor (LFSB) and a portable strip reader for rapid and sensitive quantitation of Carbohydrate Antigen 19-9 (CA 19-9) in human plasma. CA 19-9 is a biomarker that has been associated with cancers (such as pancreatic and colorectal cancers) and various non-cancerous diseases. The principle is based on sandwich-type immunoreactions between gold nanoparticle (GNP)-labelled detection antibody, anti-CA 19-9 capture antibody and CA 19-9to capture the GNPs on the test zone of LFSB...
September 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28893854/will-big-data-close-the-missing-heritability-gap
#15
Hwasoon Kim, Alexander Grueneberg, Ana I Vazquez, Stephen Hsu, Gustavo de Los Campos
Despite the important discoveries reported by Genome-Wide Association studies, for most traits and diseases the prediction R-squared (R-sq.) achieved with genetic scores remains considerably lower than the trait heritability. Modern biobanks will soon deliver unprecedentedly large biomedical data sets: Will the advent of Big Data close the gap between the trait heritability and the proportion of variance that can be explained by a genomic predictor? We addressed this question using Bayesian methods and a data analysis approach that produces a surface response relating prediction R-sq...
September 11, 2017: Genetics
https://www.readbyqxmd.com/read/28892622/gold-loaded-nanoporous-ferric-oxide-nanocubes-with-peroxidase-mimicking-activity-for-electrocatalytic-and-colorimetric-detection-of-autoantibody
#16
Mostafa Kamal Masud, Sharda Yadav, Md Nazmul Islam, Nam-Trung Nguyen, Carlos Salomon, Richard Kline, Hatem R Alamri, Zeid Abdullah Alothman, Yusuke Yamauchi, Md Shahriar A Hossain, Muhammad J A Shiddiky
Enzyme-mimicking activity of iron oxide based nanostructures have provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold loaded nanoporous ferric oxide nanocubes (Au-NPFe2O3NC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked-eye) detection of autoantibodies. The results showed that Au-NPFe2O3NC exhibits enhanced peroxidase-like activity towards the catalytic oxidation of 3,3',5,5'-tertamethylbenzidine (TMB) in the presence of H2O2 at room temperature (25°C) and follows the typical Michaelis-Menten kinetics...
September 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28891632/design-of-boron-nitride-gelatin-electrospun-nanofibers-for-bone-tissue-engineering
#17
Sakthivel Nagarajan, Habib Belaid, Celine Pochat-Bohatier, Catherine Teyssier, Igor Iatsunskyi, Emerson Coy, Sebastien Balme, David Cornu, Philippe Miele, Narayana S Kalkura, Vincent Cavailles, Mikhael Bechelany
Gelatin is a biodegradable biopolymer obtained by collagen denaturation, which shows poor mechanical properties. Hence, improving its mechanical properties is very essential towards the fabrication of efficient nontoxic material for biomedical applications. For this aim, various methods are employed using external fillers such as ceramics or bioglass. In this report, we introduce boron nitride (BN) reinforced gelatin as a new class of two dimensional biocompatible nanomaterials. The effect of the nanofiller on the mechanical behavior is analyzed...
September 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28891296/non-invasive-body-fat-burn-monitoring-from-exhaled-acetone-with-si-doped-wo3-sensing-nanoparticles
#18
Andreas T Güntner, Noriane A Sievi, S Jonathan Theodore, Tobias Gulich, Malcolm Kohler, Sotiris E Pratsinis
Obesity is a global health threat on the rise and its prevalence continues to grow. Yet suitable biomedical sensors to monitor the body fat burn rates in situ to guide physical activity or dietary interventions toward efficient weight loss are missing. Here, we introduce a compact and inexpensive breath acetone sensor based on Si-doped WO3 nanoparticles that can accurately follow body fat burn rates in real-time. We tested this sensor on 20 volunteers during exercise and rest and measured their individual breath acetone concentrations in good agreement to bench-top proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS)...
September 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28890270/solid-phase-extraction-of-the-alcohol-abuse-biomarker-phosphatidylethanol-using-newly-synthesized-polymeric-sorbent-materials-containing-quaternary-heterocyclic-groups
#19
Mariana Duarte, Kishore Kumar Jagadeesan, Johan Billing, Ecevit Yilmaz, Thomas Laurell, Simon Ekström
Phosphatidylethanol (PEth) is an interesting biomarker finding increased use for detecting long term alcohol abuse with high specificity and sensitivity. Prior to detection, sample preparation is an unavoidable step in the work-flow of PEth analysis and new protocols may facilitate it. Solid-phase extraction (SPE) is a versatile sample preparation method widely spread in biomedical laboratories due to its simplicity of use and the possibility of automation. In this work, SPE was used for the first time to directly extract PEth from spiked human plasma and spiked human blood...
August 25, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28888005/synthesis-mechanical-properties-and-corrosion-behavior-of-powder-metallurgy-processed-fe-mg2si-composites-for-biodegradable-implant-applications
#20
M Sikora-Jasinska, C Paternoster, E Mostaed, R Tolouei, R Casati, M Vedani, D Mantovani
Recently, Fe and Fe-based alloys have shown their potential as degradable materials for biomedical applications. Nevertheless, the slow corrosion rate limits their performance in certain situations. The shift to iron matrix composites represents a possible approach, not only to improve the mechanical properties, but also to accelerate and tune the corrosion rate in a physiological environment. In this work, Fe-based composites reinforced by Mg2Si particles were proposed. The initial powders were prepared by different combinations of mixing and milling processes, and finally consolidated by hot rolling...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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