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https://www.readbyqxmd.com/read/29053700/come-to-the-light-side-in-vivo-monitoring-of-pseudomonas-aeruginosa-biofilm-infections-in-chronic-wounds-in-a-diabetic-hairless-murine-model
#1
Alessandra M Agostinho Hunt, Jacob A Gibson, Casandra L Larrivee, Sandra O'Reilly, Svetlana Navitskaya, Julia V Busik, Christopher M Waters
The presence of bacteria as structured biofilms in chronic wounds, especially in diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used to understand the underlying interactions between the microorganisms and the host. The models developed to date rely on the use of haired animals and terminal collection of wound tissue for determination of viable bacteria. While significant insight has been gained with these models, this experimental procedure requires a large number of animals and sampling is time consuming...
October 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053365/hollow-internodes-permit-a-neotropical-understory-plant-to-shelter-multiple-mutualistic-ant-species-obtaining-protection-and-nutrient-provisioning-myrmecotrophy
#2
Alain Dejean, Frédéric Petitclerc, Arthur Compin, Frédéric Azémar, Bruno Corbara, Jacques H C Delabie, Céline Leroy
The Neotropical understory plant Tachia guianensis (Gentianaceae)-known to shelter the colonies of several ant species in its hollow trunks and branches-does not provide them with food rewards (e.g., extrafloral nectar). We tested whether these ants are opportunistic nesters or whether mutualistic relationships exist as for myrmecophytes or plants sheltering ant colonies in specialized hollow structures in exchange for protection from enemies and/or nutrient provisioning (myrmecotrophy). We noted 37 ant species sheltering inside T...
November 2017: American Naturalist
https://www.readbyqxmd.com/read/29053085/rifampicin-resistance-and-multidrug-resistant-tuberculosis-detection-using-xpert-mtb-rif-in-wuhan-china-a-retrospective-study
#3
Hai Huang, Yanlin Zhang, Sheng Li, Jun Wang, Jun Chen, Zhiyun Pan, Hui Gan
BACKGROUND: The Xpert MTB/RIF test (Cepheid, Sunnyvale, CA) can simultaneously detect the Mycobacterium tuberculosis (MTB) complex DNA and rifampicin (RFP) resistance and can rapidly determine RFP resistance and predict multidrug-resistant tuberculosis (MDR-TB). In this study, we analyzed clinical examination results of a hospital specializing in TB treatment in Wuhan, Hubei, China, and examined the use of traditional culture and drug-sensitive test (DST) results as a gold standard to assess the diagnosis value of the Xpert MTB/RIF test in RFP resistance and MDR-TB...
October 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29053003/detoxification-of-cancerogenic-compounds-by-lactic-acid-bacteria-strains
#4
Zhao Lili, Wei Junyan, Zhao Hongfei, Zhu Baoqing, Zhang Bolin
Carcinogens in food are an important issue that threat people's health right now. Lactic acid bacteria (LAB) strains as well-known probiotics have shown numerous perspectives in being used as a good food additive to confront cancerogenic compounds in recent years. Some LAB strains can remove cancerogenic compounds from medium environment via direct physical binding and avoid re-pollution of poisonous secondary metabolites which are generated from degradation of cancerogenic compounds. This article presents a whole overview of the physical-binding of LAB strains to such common cancerogenic compounds existed in food and feed environments as mycotoxins, polycyclic aromatic hydrocarbons (PAHs), heterocyclic amines (HAs) and pthalic acid esters (PAEs)...
October 20, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29052944/differential-expression-of-the-three-alcanivorax-borkumensis-sk2-genes-coding-for-the-p450-cytochromes-involved-in-the-assimilation-of-hydrocarbons
#5
Emma Sevilla, Luis Yuste, Renata Moreno, Fernando Rojo
Alcanivorax borkumensis, a marine bacterium highly specialized in degrading linear and branched alkanes, plays a key ecological role in the removal of marine oil spills. It contains several alternative enzyme systems for terminal hydroxylation of alkanes, including three P450 cytochromes (P450-1, P450-2 and P450-3). The present work shows cytochrome P450-1 to be expressed from the promoter of the upstream gene fdx. Promoter Pfdx was more active when C8 -C18 n-alkanes or pristane were assimilated than when pyruvate was available...
October 20, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/29052754/development-of-a-method-for-the-detection-of-polystyrene-microplastics-in-paraffin-embedded-histological-sections
#6
Cátia Gonçalves, Marta Martins, Maria H Costa, Pedro M Costa
The concerns about the presence of microplastics (MPs) in marine ecosystems have widely increased in the past years. This is reflected in a growing number of studies addressing the effects of exposure to these materials in indigenous, farmed and even laboratory marine animals subjected to toxicity-oriented bioassays. There have been, however, many constraints in the detection of MPs in biological tissues, as routine histological techniques tend to degrade these materials, which are especially sensitive to organic solvents...
October 20, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29052096/life-paths-of-patients-with-transthyretin-related-familial-amyloid-polyneuropathy-val30met-a-descriptive-study
#7
Alice Lopes, Alexandra Sousa, Isabel Fonseca, Margarida Branco, Carla Rodrigues, Teresa Coelho, Jorge Sequeiros, Paula Freitas
Transthyretin-related familial amyloid polyneuropathy Val30Met is a fatal progressive disease. It is a rare hereditary amyloidosis, manifesting as a sensorimotor neuropathy and autonomic dysfunction. It begins during adulthood and is a disabling disease, posing a great psychological burden to patients and their families. Our aim was to describe and characterize life events related to the disease and discuss its psychosocial implications. Social and demographic data and a questionnaire on history of family and personal disease, and biographic events, were applied to 209 subjects attending an outpatient specialized clinic...
October 19, 2017: Journal of Community Genetics
https://www.readbyqxmd.com/read/29051780/sensitivity-of-postnatal-fasting-plasma-glucose-in-identifying-impaired-glucose-tolerance-in-women-with-gestational-diabetes-25-years-data
#8
Habiba Kapaya, Lucy May, Richard Jacques, Priya Madhuvrata
OBJECTIVE: To assess the uptake of postnatal oral glucose tolerance test and to determine the sensitivity of fasting postnatal blood sugar in predicting 2-h impaired glucose tolerance. METHODS: Retrospective study of 1961 women diagnosed with gestational diabetes mellitus. All women were offered oral glucose tolerance test six weeks post-delivery. RESULTS: Of 1961 women, 1090 (56%) returned for postpartum oral glucose tolerance test. A fasting plasma glucose of ≥6...
September 2017: Obstetric Medicine
https://www.readbyqxmd.com/read/29051208/redefining-the-pulvinar-sign-in-fabry-disease
#9
S Cocozza, C Russo, A Pisani, G Olivo, E Riccio, A Cervo, G Pontillo, S Feriozzi, M Veroux, Y Battaglia, D Concolino, F Pieruzzi, R Mignani, P Borrelli, M Imbriaco, A Brunetti, E Tedeschi, G Palma
BACKGROUND AND PURPOSE: The pulvinar sign refers to exclusive T1WI hyperintensity of the lateral pulvinar. Long considered a common sign of Fabry disease, the pulvinar sign has been reported in many pathologic conditions. The exact incidence of the pulvinar sign has never been tested in representative cohorts of patients with Fabry disease. The aim of this study was to assess the prevalence of the pulvinar sign in Fabry disease by analyzing T1WI in a large Fabry disease cohort, determining whether relaxometry changes could be detected in this region independent of the pulvinar sign positivity...
October 19, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/29049942/bioaugmentation-of-thiabendazole-contaminated-soils-from-a-wastewater-disposal-site-factors-driving-the-efficacy-of-this-strategy-and-the-diversity-of-the-indigenous-soil-bacterial-community
#10
Evangelia S Papadopoulou, Savvas Genitsaris, Michalis Omirou, Chiara Perruchon, Anastasia Stamatopoulou, Ioannis Ioannides, Dimitrios G Karpouzas
The application of the fungicide thiabendazole (TBZ) in fruit packaging plants (FPP) results in the production of effluents which are often disposed in adjacent field sites. These require remediation to prevent further environmental dispersal of TBZ. We assessed the bioaugmentation potential of a newly isolated TBZ-degrading bacterial consortium in a naturally contaminated soil (NCS) exhibiting a natural gradient of TBZ levels (12000, 400, 250 and 12 mg kg(-1)). The effect of aging on bioaugmentation efficacy was comparatively tested in a soil with similar physicochemical properties and soil microbiota, which was artificially, contaminated with the same TBZ levels (ACS)...
October 16, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29048701/medical-devices-clinical-chemistry-and-clinical-toxicology-devices-classification-of-the-organophosphate-test-system-final-order
#11
(no author information available yet)
The Food and Drug Administration (FDA or we) is classifying the organophosphate test system into class II (special controls). The special controls that apply to the device type are identified in this order and will be part of the codified language for the organophosphate test system's classification. We are taking this action because we have determined that classifying the device into class II (special controls) will provide a reasonable assurance of safety and effectiveness of the device. We believe this action will also enhance patients' access to beneficial innovative devices, in part by reducing regulatory burdens...
October 18, 2017: Federal Register
https://www.readbyqxmd.com/read/29047803/light-efficient-augmented-reality-3d-display-using-highly-transparent-retro-reflective-screen
#12
Shoaib R Soomro, Hakan Urey
We propose and demonstrate a light-efficient 3D display using a highly transparent desktop-sized augmented reality screen. The display consists of a specially designed transparent retro-reflective screen and a pair of low-power pico-projectors positioned close to the viewer's eyes to provide stereo views. The transfer screen is an optically clear sheet partially patterned with retro-reflective microspheres for high optical gain. The retro-reflective material buried in the screen reflects incident light back towards the projectors with a narrow scattering angle and facilitates the viewer to perceive a very bright content...
August 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047664/dynamic-wavefront-measurement-with-a-pinhole-linear-polarizer-point-diffraction-interferometer
#13
Xiang Zhou, Renhui Guo, Wenhua Zhu, Donghui Zheng, Lei Chen
We propose a pinhole linear polarizer point-diffraction interferometer (PLP-PDI) for dynamic wavefront measurements. The proposed interferometer uses a metallic wire grid linear polarizer that acts as a point-diffraction plate to generate an ideal spherical wave, from which we can obtain orthogonally polarized reference and test beams. The special polarization phase-shifting configuration allows four phase-shifted interferograms to be captured in a single shot with high precision and stability. The wavefront can then be reconstructed using a phase-unwrapping algorithm...
October 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047661/highly-concentrated-ring-shaped-phase-conversion-laser-induced-breakdown-spectroscopy-technology-for-liquid-sample-analysis
#14
Qingyu Lin, Zhimei Wei, Hongli Guo, Shuai Wang, Guangmeng Guo, Zhi Zhang, Yixiang Duan
A highly concentrated, ring-shaped phase conversion (RSPC) method was developed for liquid sample analysis using the laser-induced breakdown spectroscopy (LIBS) technique. In this work, test samples were prepared by mixing the metal particles with polyvinyl alcohol (PVA) supporter in liquid phase. With heat, the PVA solution solidified inside a modified glass petri dish, forming a metal-enriched polymer ring film. Distinguished from other traditional liquid-to-solid conversing methods, the proposed new method takes advantage of enhanced homogeneity for the target elements inside the ring film...
June 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/29046823/functionalization-of-ti-40nb-implant-material-with-strontium-by-reactive-sputtering
#15
Markus Göttlicher, Marcus Rohnke, Yannik Moryson, Jürgen Thomas, Joachim Sann, Anja Lode, Matthias Schumacher, Romy Schmidt, Stefan Pilz, Annett Gebert, Thomas Gemming, Jürgen Janek
BACKGROUND: Surface functionalization of orthopedic implants with pharmaceutically active agents is a modern approach to enhance osseointegration in systemically altered bone. A local release of strontium, a verified bone building therapeutic agent, at the fracture site would diminish side effects, which could occur otherwise by oral administration. Strontium surface functionalization of specially designed titanium-niobium (Ti-40Nb) implant alloy would provide an advanced implant system that is mechanically adapted to altered bone with the ability to stimulate bone formation...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/29046046/three-dimensional-evaluation-of-similarity-of-right-and-left-knee-joints
#16
Ki-Mo Jang, Jong-Hoon Park, Minho Chang, Youngjun Kim, Deukhee Lee, Sehyung Park, Joon Ho Wang
Purpose: The purpose of this study was to evaluate the anatomical similarity of three-dimensional (3D) morphometric parameters between right and left knees. Materials and Methods: Ten fresh-frozen paired cadaveric knees were tested. Following dissection, footprint areas of the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) were measured. Surface scanning was performed using a 3D scanner. Scanned data were reproduced and morphometric parameters were measured on specialized software...
October 19, 2017: Knee Surgery & related Research
https://www.readbyqxmd.com/read/29045194/testing-an-aac-system-that-transforms-pictograms-into-natural-language-with-persons-with-cerebral-palsy
#17
Joan Pahisa-Solé, Jordi Herrera-Joancomartí
In this paper, we describe a compansion system that transforms the telegraphic language that comes from the use of pictogram-based Augmentative and Alternative Communication (AAC) into natural language. The system was tested with 4 participants with severe cerebral palsy and ranging degrees of linguistic competence and intellectual disabilities. Participants had used pictogram-based AAC at least for the past 30 years each and presented a stable linguistic profile. During tests, which consisted in a total of 40 sessions, participants were able to learn new linguistic skills, such as the use of basic verb tenses, while using the compansion system, which proved a source of motivation...
October 18, 2017: Assistive Technology: the Official Journal of RESNA
https://www.readbyqxmd.com/read/29045063/evaluating-manufacturing-process-profile-comparability-with-multivariate-equivalence-testing-case-study-of-cell-culture-small-scale-model-transfer
#18
Yang Cao, Daniel Obeng, Guodong Hui, Luting Xue, Yukun Ren, Xianjie Yu, Fei Wang, Chad Atwell
This paper studies the Generalized Mahalanobis Distance (GMD) approach proposed by Hoffelder(1) which measures the dissimilarity of two multivariate Gaussian distributions with arbitrary covariance matrices and unequal sample sizes. This investigation demonstrated that, with appropriate adjustment, the GMD approach can achieve the targeted nominal Type I error and provide sufficient power for testing equivalence between two profile populations. The adjusted GMD approach was applied to examine the equivalence of harvest profiles between a 12L small scale model and 2000L manufacturing scale in a transfer study performed at Sanofi Speciality Care Framingham Biologics...
October 16, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29043034/sea-level-rise-may-increase-extinction-risk-of-a-saltmarsh-ontogenetic-habitat-specialist
#19
David Samuel Johnson, Bethany L Williams
Specialist species are more vulnerable to environmental change than generalist species. For species with ontogenetic niche shifts, specialization may occur at a particular life stage making those stages more susceptible to environmental change. In the salt marshes in the northeast U.S., accelerated sea level rise is shifting vegetation patterns from flood-intolerant species such as Spartina patens to the flood-tolerant Spartina alterniflora. We tested the potential impact of this change on the coffee bean snail, Melampus bidentatus, a numerically dominant benthic invertebrate with an ontogenetic niche shift...
October 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29039345/validation-of-transition-readiness-assessment-questionaire-in-turkish-adolescents-with-diabetes
#20
Evrim Kızıler, Dilek Yıldız, Berna Eren Fidancı
BACKGROUND: Today, more than 90% of adolescents with chronic conditions are surviving into adulthood and move pediatric to adult care with their chronic illness. It is important to gain autonomy and ensure readiness for adolescents/ young adults to use the adult health care system prior to the transfer of care. However, the lack of the transition readiness assessment tool which is validated, patient-centered and appropriate to developmental differences in pediatric care is seen a major obstacle for transition of care from pediatric to adult services...
October 17, 2017: Balkan Medical Journal
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