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https://www.readbyqxmd.com/read/29148924/influence-of-gestational-diabetes-on-the-activity-of-%C3%AE-aminolevulinate-dehydratase-and-oxidative-stress-biomarkers
#1
Fabiane Rodrigues, Leidiane de Lucca, Walter S Neme, Thissiane de Lima Gonçalves
OBJECTIVES: This study aimed to evaluate the activity of delta-aminolevulinate dehydratase (δ-ALA-D) and oxidative stress biomarkers in pregnant women with gestational diabetes mellitus (GDM), in order to demonstrate the involvement of oxidative stress in this condition, which presents pathophysiology still undetermined. METHODS: δ-ALA-D activity, lipid peroxidation estimated as the levels of thiobarbituric acid reactive substances (TBARS), protein (P-SH) and non-protein thiol (NP-SH) content, catalase (CAT) activity and concentration of vitamin C (VIT C) in samples of pregnant women with GDM (n = 48) and in healthy pregnant women (n = 30), who constituted the control group...
November 17, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/29075077/bone-histo-morphology-in-chronic-kidney-disease-mineral-bone-disorder
#2
Khuraijam Bembem, Tejinder Singh, Narinder Pal Singh, Alpana Saxena, Shyama Lata Jain
Chronic Kidney Disease-Mineral Bone Disorder(CKD-MBD) is a systemic disorder of the mineral and bone metabolism seen in patients with Chronic Kidney Disease(CKD). It is manifested by either one or a combination of the following: (a) Abnormalities of calcium, phosphorus, PTH, or vitamin D metabolism. (b) Abnormalities in bone turnover, mineralization, volume, linear growth, or strength. (c) Vascular or other soft- tissue calcification. Renal osteodystrophy measures the skeletal component of CKD-MBD. To study the histomorphology of bone marrow biopsy in patients with CKD-MBD and correlate the histological features of bone biopsy with the clinicobiochemical parameters...
December 2017: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29068430/systemic-inflammation-oxidative-damage-to-nucleic-acids-and-metabolic-syndrome-in-the-pathogenesis-of-psoriasis
#3
Lenka Borska, Jan Kremlacek, Ctirad Andrys, Jan Krejsek, Kvetoslava Hamakova, Pavel Borsky, Vladimir Palicka, Vit Rehacek, Andrea Malkova, Zdenek Fiala
In the pathogenesis of psoriasis, systemic inflammation and oxidative stress play mutual roles interrelated with metabolic syndrome (MetS). This study aims to map the selected markers of inflammation (C-reactive protein (CRP)), oxidative damage to nucleic acids (DNA/RNA damage; 8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), and the parameters of MetS (waist circumference, fasting glucose, triglycerides, high-density lipoprotein (HDL) cholesterol, diastolic and systolic blood pressure) in a group of 37 patients with psoriasis (62% of MetS) and in 43 healthy controls (42% of MetS)...
October 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29063748/new-data-on-chronic-venous-disease-a-new-place-for-cyclo-3%C3%A2-fort
#4
Stravos K Kakkos, Eliete Bouskela, Arkadiusz Jawien, Andrew N Nicolaides
With our increasing knowledge of the epidemiology, pathophysiology, investigation and clinical aspects of chronic venous disease (CVD) and new data on the various therapies available, an update of the recommendations on CVD and its management appears to be necessary. The symposium "New Data on Chronic Venous Disease, New Place for Cyclo 3® Fort", held at the annual meeting of the European Venous Forum (EVF) on June 30th, 2017 in Porto, Portugal, reported the recent developments on Ruscus+HMC+Vit C (Cyclo 3® Fort), including the results of a series of in vivo pharmacological experiments and a recent meta-analysis...
October 23, 2017: International Angiology: a Journal of the International Union of Angiology
https://www.readbyqxmd.com/read/29054518/a-statistical-approach-to-optimize-the-production-of-polyhydroxyalkanoates-from-wickerhamomyces-anomalus-vit-nn01-using-response-surface-methodology
#5
Nupur Ojha, Nilanjana Das
Polyhydroxyalkanoates (PHAs) are three-level group of biodegradable polymers and attractive substitutes over conventional plastics to avoid the pollution problems. The yeast strain isolated from sugarcane juice, identified as Wickerhamomyces anomalus VIT-NN01, was used for the production of polyhydroxyalkanoates (PHA). Response surface methodology (RSM), three-level six variables Box-Behnken design (BBD), was employed to optimize the factors such as pH 8.0, temperature 37°C, sugarcane molasses (35g/L) supplemented with co-substrate palm oil (0...
October 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29052324/effect-of-heat-stress-during-in-vitro-maturation-on-developmental-competence-of-vitrified-bovine-oocytes
#6
M Vendrell-Flotats, N Arcarons, E Barau, M López-Béjar, T Mogas
Heat-shock protein 70 (HSP70) plays a crucial role as intracellular cytoprotectant and molecular chaperone. A phenomenon of heat stress (HS) leads to production of these proteins that could be beneficial to cells during cryopreservation, which is also a stressful process for the cell. This study aimed to evaluate the protective effect of exposure of bovine oocytes to moderate HS during in vitro maturation (IVM) prior vitrification. First, oocytes were subjected to HS (41.5°C for 1 hr) at 0, 4, 8, 12 or 16 of IVM...
October 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29040674/evaluation-of-apoptotic-and-autophagic-related-protein-expressions-before-and-after-ivm-of-fresh-slow-frozen-and-vitrified-pre-pubertal-mouse-testicular-tissue
#7
L Dumont, F Chalmel, A Oblette, B Berby, A Rives, V Duchesne, C Rondanino, N Rives
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i...
November 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29020506/potential-application-of-immobilized-streptokinase-extracted-from-streptococcus-equinus-vit_vb2
#8
B Vaishnavi, C Subathra Devi
Streptokinase purified from Streptococcus equinus VIT_VB2 isolated from bovine milk sample was immobilized in various solid supports namely entrapment in agarose gel, calcium alginate beads and gelatin gel by crosslinking with formaldehyde. Immobilization of streptokinase in calcium alginate beads showed maximum efficiency (81.8 ± 1.06%) when compared to entrapment with agarose gel (55.6 ± 2.17%) and cross-linked gelatin formaldehyde gel (71.0 ± 1.54%). The purified SK activity was expressed maximum in calcium alginate (1%) and gelatin gel (0...
October 11, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28976847/effect-of-light-activation-of-pulp-capping-materials-and-resin-composite-on-dentin-deformation-and-the-pulp-temperature-change
#9
C J Soares, M S Ferreira, A A Bicalho, M de Paula Rodrigues, Ssl Braga, A Versluis
OBJECTIVES: To analyze the effect of pulp-capping materials and resin composite light activation on strain and temperature development in the pulp and on the interfacial integrity at the pulpal floor/pulp-capping materials in large molar class II cavities. METHODS: Forty extracted molars received large mesio-occlusal-distal (MOD) cavity bur preparation with 1.0 mm of dentin remaining at the pulp floor. Four pulp-capping materials (self-etching adhesive system, Clearfil SE Bond [CLE], Kuraray), two light-curing calcium hydroxide cements (BioCal [BIO], Biodinâmica, and Ultra-Blend Plus [ULT], Ultradent), and a resin-modified glass ionomer cement- (Vitrebond [VIT], 3M ESPE) were applied on the pulpal floor...
October 4, 2017: Operative Dentistry
https://www.readbyqxmd.com/read/28954710/mechanisms-of-anti-cancer-effects-of-ascorbate-cytotoxic-activity-and-epigenetic-modulation
#10
REVIEW
Domenico Mastrangelo, Elvira Pelosi, Germana Castelli, Francesco Lo-Coco, Ugo Testa
Vitamin C (Vit C or Ascorbate) is essential for many fundamental biochemical processes. Vit C is an essential nutrient with redox functions at normal physiologic concentrations. The main physiologic function of this vitamin is related to its capacity to act as a co-factor for a large family of enzymes, collectively known as Fe and 2-oxoglutarate-dependent dioxygenases. It also modulates epigenetic gene expression through the control of TET enzymes activity. Vit C also has several biological properties allowing to restore the deregulated epigenetic response observed in many tumors...
September 21, 2017: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/28949229/first-measurement-of-transverse-spin-dependent-azimuthal-asymmetries-in-the-drell-yan-process
#11
M Aghasyan, R Akhunzyanov, G D Alexeev, M G Alexeev, A Amoroso, V Andrieux, N V Anfimov, V Anosov, A Antoshkin, K Augsten, W Augustyniak, A Austregesilo, C D R Azevedo, B Badełek, F Balestra, M Ball, J Barth, R Beck, Y Bedfer, J Bernhard, K Bicker, E R Bielert, R Birsa, M Bodlak, P Bordalo, F Bradamante, A Bressan, M Büchele, W-C Chang, C Chatterjee, M Chiosso, I Choi, S-U Chung, A Cicuttin, M L Crespo, S Dalla Torre, S S Dasgupta, S Dasgupta, O Yu Denisov, L Dhara, S V Donskov, N Doshita, Ch Dreisbach, W Dünnweber, M Dziewiecki, A Efremov, P D Eversheim, M Faessler, A Ferrero, M Finger, M Finger, H Fischer, C Franco, N du Fresne von Hohenesche, J M Friedrich, V Frolov, E Fuchey, F Gautheron, O P Gavrichtchouk, S Gerassimov, J Giarra, F Giordano, I Gnesi, M Gorzellik, A Grasso, M Grosse Perdekamp, B Grube, T Grussenmeyer, A Guskov, D Hahne, G Hamar, D von Harrach, F H Heinsius, R Heitz, F Herrmann, N Horikawa, N d'Hose, C-Y Hsieh, S Huber, S Ishimoto, A Ivanov, Yu Ivanshin, T Iwata, V Jary, R Joosten, P Jörg, E Kabuß, A Kerbizi, B Ketzer, G V Khaustov, Yu A Khokhlov, Yu Kisselev, F Klein, J H Koivuniemi, V N Kolosov, K Kondo, K Königsmann, I Konorov, V F Konstantinov, A M Kotzinian, O M Kouznetsov, Z Kral, M Krämer, P Kremser, F Krinner, Z V Kroumchtein, Y Kulinich, F Kunne, K Kurek, R P Kurjata, A Kveton, A A Lednev, M Levillain, S Levorato, Y-S Lian, J Lichtenstadt, R Longo, A Maggiora, A Magnon, N Makins, N Makke, G K Mallot, B Marianski, A Martin, J Marzec, J Matoušek, H Matsuda, T Matsuda, G V Meshcheryakov, M Meyer, W Meyer, Yu V Mikhailov, M Mikhasenko, E Mitrofanov, N Mitrofanov, Y Miyachi, A Nagaytsev, F Nerling, D Neyret, J Nový, W-D Nowak, G Nukazuka, A S Nunes, A G Olshevsky, I Orlov, M Ostrick, D Panzieri, B Parsamyan, S Paul, J-C Peng, F Pereira, M Pešek, D V Peshekhonov, N Pierre, S Platchkov, J Pochodzalla, V A Polyakov, J Pretz, M Quaresma, C Quintans, S Ramos, C Regali, G Reicherz, C Riedl, N S Rogacheva, M Roskot, D I Ryabchikov, A Rybnikov, A Rychter, R Salac, V D Samoylenko, A Sandacz, C Santos, S Sarkar, I A Savin, T Sawada, G Sbrizzai, P Schiavon, K Schmidt, H Schmieden, K Schönning, E Seder, A Selyunin, O Yu Shevchenko, L Silva, L Sinha, S Sirtl, M Slunecka, J Smolik, A Srnka, D Steffen, M Stolarski, O Subrt, M Sulc, H Suzuki, A Szabelski, T Szameitat, P Sznajder, S Takewaka, M Tasevsky, S Tessaro, G Terça, F Tessarotto, A Thiel, J Tomsa, F Tosello, V Tskhay, S Uhl, A Vauth, J Veloso, M Virius, M Vit, J Vondra, S Wallner, T Weisrock, M Wilfert, J Ter Wolbeek, K Zaremba, P Zavada, M Zavertyaev, E Zemlyanichkina, N Zhuravlev, M Ziembicki
The first measurement of transverse-spin-dependent azimuthal asymmetries in the pion-induced Drell-Yan (DY) process is reported. We use the CERN SPS 190  GeV/c π^{-} beam and a transversely polarized ammonia target. Three azimuthal asymmetries giving access to different transverse-momentum-dependent (TMD) parton distribution functions (PDFs) are extracted using dimuon events with invariant mass between 4.3  GeV/c^{2} and 8.5  GeV/c^{2}. Within the experimental uncertainties, the observed sign of the Sivers asymmetry is found to be consistent with the fundamental prediction of quantum chromodynamics (QCD) that the Sivers TMD PDFs extracted from DY have a sign opposite to the one extracted from semi-inclusive deep-inelastic scattering (SIDIS) data...
September 15, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28946183/cardioprotective-effect-of-phyllanthus-amarus-against-high-fructose-diet-induced-myocardial-and-aortic-stress-in-rat-model
#12
Mallaiah Putakala, Sudhakara Gujjala, Srinivasulu Nukala, Sasi Bhusana Rao Bongu, Nagaraju Chintakunta, Saralakumari Desireddy
Increased number of population with heart stroke/attack is attributed to sedentary lifestyle and consumption of high-sugar diets, especially fructose. The objective of this study is to investigate the cardio-protective activity of aqueous extract of Phyllanthus amarus (PAAE) against high-fructose (HF) diet induced cardiac damage in Wistar rats. Male Wistar rats were randomly assigned into five groups of six animals each: Control (C), Control treated with PAAE (C+PAAE), High fructose diet fed (F), High fructose diet fed treated with PAAE (F+PAAE) and High fructose diet fed treated with Pioglitazone (F+Pio)...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28943597/changes-of-klotho-protein-and-klotho-mrna-expression-in-a-hydroxy-l-proline-induced-hyperoxaluric-rat-model
#13
Orapun Jaturakan, Chollada Buranakarl, Thasinas Dissayabutra, Narongsak Chaiyabutr, Anusak Kijtawornrat, Anudep Rungsipipat
Klotho protein is recognized as having a renoprotective effect and is used as a biomarker for kidney injury. We investigated the level of Klotho protein in hyperoxaluria-induced kidney injury and the effects of vitamin E (Vit E) and vitamin C (Vit C) supplementation. Hyperoxaluria was induced by feeding 2% (w/v) Hydroxy-L-proline (HLP) in the drinking water for 21 days. Rats were divided into 5 groups; control (Group 1, n=7), HLP treated rats that received nothing else (Group 2, n=7), Vit E (Group 3, n=6), Vit C (Group 4, n=6) and both Vit E and Vit C (Group 5, n=7)...
September 23, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28912655/chronic-eccentric-exercise-and-antioxidant-supplementation-effects-on-lipid-profile-and-insulin-sensitivity
#14
Christina Yfanti, Athanasios Tsiokanos, Ioannis G Fatouros, Anastasios A Theodorou, Chariklia K Deli, Yiannis Koutedakis, Athanasios Z Jamurtas
Eccentric exercise has been shown to exert beneficial effects in both lipid profile and insulin sensitivity. Antioxidant supplementation during chronic exercise is controversial as it may prevent the physiological training-induced adaptations. The aim of this study was to investigate: 1) the minimum duration of the eccentric exercise training required before changes on metabolic parameters are observed and 2) whether antioxidant supplementation during training would interfere with these adaptations. Sixteen young healthy men were randomized into the Vit group (1 g of vitamin C and 400 IU vitamin E daily) and the placebo (PL) group...
September 2017: Journal of Sports Science & Medicine
https://www.readbyqxmd.com/read/28879460/vitamin-b6-reduces-neurochemical-and-long-term-cognitive-alterations-after-polymicrobial-sepsis-involvement-of-the-kynurenine-pathway-modulation
#15
Lucinéia Gainski Danielski, Amanda Della Giustina, Mariana Pereira Goldim, Drielly Florentino, Khiany Mathias, Leandro Garbossa, Rosiane de Bona Schraiber, Ana Olívia Martins Laurentino, Marina Goulart, Monique Michels, Karina Barbosa de Queiroz, Markus Kohlhof, Gislaine Tezza Rezin, Jucélia Jeremias Fortunato, Joao Quevedo, Tatiana Barichello, Felipe Dal-Pizzol, Roney S Coimbra, Fabricia Petronilho
Neurological dysfunction as a result of neuroinflammation has been reported in sepsis and cause high mortality. High levels of cytokines stimulate the formation of neurotoxic metabolites by kynurenine (KYN) pathway. Vitamin B6 (vit B6) has anti-inflammatory and antioxidant properties and also acts as a cofactor for enzymes of the KYN pathway. Thus, by using a relevant animal model of polymicrobial sepsis, we studied the effect of vit B6 on the KYN pathway, acute neurochemical and neuroinflammatory parameters, and cognitive dysfunction in rats...
September 6, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28826225/low-oxidative-stress-mediated-proliferation-via-jnk-foxo3a-catalase-signaling-in-transplanted-adult-stem-cells-promotes-wound-tissue-regeneration
#16
Neha R Dhoke, Ramasatyaveni Geesala, Amitava Das
AIMS: Stem cells exposed to pathological levels of reactive oxygen species (ROS) at wound sites fail to regenerate tissue. The molecular mechanism underlying differential levels of ROS-mediated regulation of stem cells remains elusive. This study elucidates the mechanistic role of catalase at 10 μM H2O2-induced proliferation of mouse bone marrow stromal (BMSC) and hematopoietic (HSPC) stem/progenitor cells. RESULTS: BMSCs and HSPCs depicted an increased growth rate and colony formation, in the presence of 10 μM but not 100 μM concentration of H2O2, an effect that was perturbed by Vit...
October 4, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28815782/influence-of-conjugated-linoleic-acids-and-vitamin-e-on-milk-fatty-acid-composition-and-concentrations-of-vitamin-a-and-%C3%AE-tocopherol-in-blood-and-milk-of-dairy-cows
#17
S Schäfers, U Meyer, D von Soosten, L Hüther, C Drong, K Eder, E Most, A Tröscher, W Pelletier, A Zeyner, S Dänicke
The objective of this trial was to investigate the influences of conjugated linoleic acid (CLA) and vitamin E (Vit. E) and their interactions on fatty acid composition and vitamins in milk (α-tocopherol, retinol and β-carotene) as well as on α-tocopherol in blood of pluriparous cows from week 6 ante partum until week 10 post-partum (p.p.). We assigned 59 pluriparous German Holstein cows to four treatment groups with the treatment factors CLA and Vit. E at two levels in a 2 × 2 factorial design. Milk fatty acid composition and milk vitamins were analysed on lactation days 7 and 28...
August 16, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28805603/modern-human-sperm-freezing-effect-on-dna-chromatin-and-acrosome-integrity
#18
Tahereh Rahiminia, Akram Hosseini, Morteza Anvari, Saeed Ghasemi-Esmailabad, Ali Reza Talebi
OBJECTIVE: Presence of vitrification method in sperm freezing and the introduction of solid surface vitrification beside rapid freezing in vapour, opens an easy and safe way to help infertility centres. While the effects of cryopreservation on motility, morphology and viability of sperm are documented, the question of the probable alteration of sperm DNA, chromatin and acrosome integrity after freezing and thawing procedures in different methods is still controversial. MATERIALS AND METHODS: Normal sample were collected according to WHO strict criteria...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28759296/potential-role-of-l-arginine-and-vitamin-e-against-bone-loss-induced-by-nano-zinc-oxide-in-rats
#19
Hala M Abdelkarem, Laila H Fadda, Eman M El-Sayed, Omyma K Radwan
The purpose of this study was to illustrate the effects of zinc oxide nanoparticles (ZnO-NPs) administration on bone turnover and bone resorbing agents in rats and how L-arginine (L-arg) or vitamin E (vit E) co-administrations might affect them. Fasting rats were randomly divided into four groups (n = 10): G1-normal healthy animals; G2-ZnO-NPs-exposed rats (600 mg/kg(-)1/day(-1)); G3-ZnO-NPs-exposed rats co-administrated L-arg (200 mg/kg(-)1/day(-1)); G4-ZnO-NPs-exposed rats co-administrated vit E (200 mg/kg(-)1/day(-1))...
July 31, 2017: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/28737734/storage-of-fruits-and-vegetables-in-refrigerator-increases-their-phenolic-acids-but-decreases-the-total-phenolics-anthocyanins-and-vitamin-c-with-subsequent-loss-of-their-antioxidant-capacity
#20
Joseph H Y Galani, Jalpesh S Patel, Nilesh J Patel, Jayant G Talati
It is of paramount importance for consumers, scientists and industrialists to understand how low-temperature storage of food items affects their bioactive compounds and properties. This study evaluated the effects of cold storage on total phenolics (TP), phenolic acids profile (PA), total anthocyanins (TA), total ascorbic acid (Vit. C) and antioxidant activity (AA) of 19 fruits and vegetables, collected from local Indian markets and stored in refrigerator (4 °C) during 15 days. Content of TP was highest in dill and amaranth and decreased (up to 29...
July 24, 2017: Antioxidants (Basel, Switzerland)
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