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https://www.readbyqxmd.com/read/27888111/neutralizing-human-recombinant-antibodies-against-herpes-simplex-virus-type-1-glycoproteins-b-from-a-phage-displayed-scfv-antibody-library
#1
Vahid Bagheri, Foroogh Nejatollahi, Seyed Alireza Esmaeili, Amir Abbas Momtazi, Mohamad Motamedifar, Amirhossein Sahebkar
The HSV-1 envelope glycoprotein B (gB) plays a critical role in virus entry into host cells. Neutralizing antibodies can therefore potentially prevent virus entry into target cells and cell-to-cell spread of infection. Our present study focused on the selection of neutralizing single-chain Fv (scFv) antibodies of a phage-displayed nonimmune human scFv antibody library against gB of HSV-1. To enrich specific scFvs, two phage antibodies were isolated against amino acid residues 31-43 derived from the N-terminal part of gB using panning technique...
November 22, 2016: Life Sciences
https://www.readbyqxmd.com/read/27879646/integrating-scfv-into-xmap-assays-for-the-detection-of-marine-toxins
#2
Lisa C Shriver-Lake, Jinny L Liu, P Audrey Brozozog Lee, Ellen R Goldman, Richard Dietrich, Erwin Märtlbauer, George P Anderson
Marine toxins, such as saxitoxin and domoic acid are associated with algae blooms and can bioaccumulate in shell fish which present both health and economic concerns. The ability to detect the presence of toxin is paramount for the administration of the correct supportive care in case of intoxication; environmental monitoring to detect the presence of toxin is also important for prevention of intoxication. Immunoassays are one tool that has successfully been applied to the detection of marine toxins. Herein, we had the variable regions of two saxitoxin binding monoclonal antibodies sequenced and used the information to produce recombinant constructs that consist of linked heavy and light variable domains that make up the binding domains of the antibodies (scFv)...
November 21, 2016: Toxins
https://www.readbyqxmd.com/read/27849134/developing-and-characterization-of-single-chain-variable-fragment-scfv-antibody-against-frizzled-7-fzd7-receptor
#3
Hamid Nickho, Vahid Younesi, Leili Aghebati-Maleki, Morteza Motallebnezhad, Jafar Majidi Zolbanin, Aliakbar Movassagh Pour, Mehdi Yousefi
Wnt/β-catenin signaling pathway through Frizzled receptors has been shown to play a key role in both normal development and tumorigenesis. Overexpression of Wnt pathway genes, such as Fzd7 in several malignancies is well-documented. Therefore, targeting of Fzd7 and its ligand inhibits cancer cells proliferation metastasis. In the present study we isolated single chain variable fragments (scFvs) against Fzd7 receptor using phage display method. Semi-synthetic human naive antibody libraries (Tomlinson I + J) was employed in panning procedure to isolate specific scFv against specific peptide from extracellular domain of Fzd7 receptor...
November 16, 2016: Bioengineered
https://www.readbyqxmd.com/read/27829339/improved-brain-expression-of-anti-amyloid-%C3%AE-scfv-by-complexation-of-mrna-including-a-secretion-sequence-with-peg-based-block-catiomer
#4
Federico Perche, Satoshi Uchida, Hiroki Akiba, Chin-Yu Lin, Masaru Ikegami, Anjaneyulu Dirisala, Toshihiro Nakashima, Keiji Itaka, Kohei Tsumoto, Kazunori Kataoka
Amyloid β aggregates have been proposed as central effectors in Alzheimer's disease, as evidenced by several passive immunotherapies in clinical trials. We used mRNA encoding anti-amyloid β (Aβ) scFv for passive immunotherapy. These scFvs could recognize amyloid oligomers with significant affinity, delay the aggregation of amyloid β in vitro, and dissociate Aβ aggregates in vitro. Intra-cerebroventricular injection of pDNA and mRNA encoding the scFv resulted in detectable scFv levels in the brain, with higher in vivo expression from mRNA...
November 8, 2016: Current Alzheimer Research
https://www.readbyqxmd.com/read/27805609/bacterial-inner-membrane-display-for-screening-a-library-of-antibody-fragments
#5
Parisa Moghaddam-Taaheri, Svetlana P Ikonomova, Zifan Gong, Janna Q Wisniewski, Amy J Karlsson
Antibodies engineered for intracellular function must not only have affinity for their target antigen, but must also be soluble and correctly folded in the cytoplasm. Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. Here, we describe a protocol to screen a recombinant library of single-chain variable fragment (scFv) antibodies for antigen-binding and proper cytoplasmic folding simultaneously...
October 15, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27786270/recombinant-antibodies-for-specific-detection-of-clostridial-fe-fe-hydrogenases
#6
Rahul Mangayil, Matti Karp, Urpo Lamminmäki, Ville Santala
Biological hydrogen production is based on activity of specific enzymes called hydrogenases. Hydrogenases are oxygen sensitive metalloenzymes containing Ni and/or Fe atoms at the active site, catalyzing reversible reduction of protons. Generally, [Fe-Fe] hydrogenases prefer proton reduction to molecular hydrogen, a potential energy carrier molecule that can be produced by bioprocesses in sustainable manner. Thus, monitoring tools have been developed to study the relationship between [Fe-Fe] hydrogenases and biohydrogen production in bioreactors at DNA and RNA levels...
October 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27762541/nanoyeast-and-other-cell-envelope-compositions-for-protein-studies-and-biosensor-applications
#7
Yadveer S Grewal, Muhammad J A Shiddiky, Stephen M Mahler, Gerard A Cangelosi, Matt Trau
Rapid progress in disease biomarker discovery has increased the need for robust detection technologies. In the past several years, the designs of many immunoaffinity reagents have focused on lowering costs and improving specificity while also promoting stability. Antibody fragments (scFvs) have long been displayed on the surface of yeast and phage libraries for selection; however, the stable production of such fragments presents challenges that hamper their widespread use in diagnostics. Membrane and cell wall proteins similarly suffer from stability problems when solubilized from their native environment...
November 4, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27712994/identification-of-high-affinity-anti-cd16a-allotype-independent-human-antibody-domains
#8
Wei Li, Hongjia Yang, Dimiter S Dimitrov
CD16A (FcγRIIIA) is an activating receptor mostly expressed on natural killer (NK) cells and monocytes/macrophages. It can mediate antibody-dependent cell-mediated cytotoxicity (ADCC) through low-affinity interaction with human immunoglobulin G (IgG) Fc. It can also mediate cell lysis if NK cells are guided by bispecific killer cells engagers (BiKEs). BiKEs showed some success in clinical trials of cancer and are promising candidate therapeutics. However, currently reported BiKEs are based on antibody fragments (scFvs) of relatively large size...
October 3, 2016: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/27692095/screening-scfv-antibodies-against-infectious-bursal-disease-virus-by-co-expression-of-antigen-and-antibody-in-the-bacteria-display-system
#9
Xiaochen Guo, Hongxue Cao, Yunxin Wang, Yang Liu, Yanmin Chen, Nan Wang, Shan Jiang, Shengqi Zhang, Qiang Wu, Tianhe Li, Yingjie Zhang, Bing Zhou, Jiechao Yin, Deshan Li, Guiping Ren
We have previously reported an antigen and antibody co-expression (AAC) technology to demonstrate the interaction between a known antigen and antibody. To validate the co-expression system for screening antibody libraries, a single chain fragment variable(scFv)antibody library was constructed from chickens immunized with the VP2 protein of infectious bursal disease virus (IBDV). The genes of both VP2 and scFv antibodies were inserted into the pBFD-Ab-Ag vector. The co-expression library was subjected to three rounds of screening by flow cytometry (FCM) using a polyclonal antibody against VP2 through a bacteria display system...
November 1, 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27650544/tetraspecific-scfv-construct-provides-nk-cell-mediated-adcc-and-self-sustaining-stimuli-via-insertion-of-il-15-as-a-cross-linker
#10
Joerg U Schmohl, Martin Felices, Deborah Todhunter, Elizabeth Taras, Jeffrey S Miller, Daniel A Vallera
BACKGROUND: The design of a highly effective anti-cancer immune-engager would include targeting of highly drug refractory cancer stem cells (CSC). The design would promote effective antibody-dependent cell-mediated cytotoxicity (ADCC) and simultaneously promote costimulation to expand and self-sustain the effector NK cell population. Based on our bispecific NK cell engager platform we constructed a tetraspecific killer engager (TetraKE) comprising single-chain variable fragments (scFvs) binding FcγRIII (CD16) on NK cells, EpCAM on carcinoma cells and CD133 on cancer stem cells in order to promote ADCC...
September 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27639384/antiproliferative-and-apoptotic-effects-of-a-specific-anti-insulin-like-growth-factor-i-receptor-single-chain-antibody-on-breast-cancer-cells
#11
Morteza Motallebnezhad, Vahid Younesi, Leili Aghebati-Maleki, Hamid Nickho, Elham Safarzadeh, Majid Ahmadi, Ali Akbar Movassaghpour, Ahmad Hosseini, Mehdi Yousefi
Insulin-like growth factor I receptor (IGF-IR) is expressed on breast cancer cells and involves in metastasis, survival, and proliferation. Currently, application of IGF-IR-targeting monoclonal antibodies (mAbs), alone or in combination with other drugs, is a promising strategy for breast cancer therapy. Single-chain fragment variable (scFv) antibodies have been introduced as appropriate tools for tumor-targeting purposes because of their advantages over whole antibodies. In the present study, we employed a naïve phage library and isolated scFvs against a specific epitope from extracellular domain of IGF-IR by panning process...
September 17, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27638305/human-transbodies-to-vp40-inhibit-cellular-egress-of-ebola-virus-like-particles
#12
Salma Teimoori, Watee Seesuay, Surasak Jittavisutthikul, Urai Chaisri, Nitat Sookrung, Jaslan Densumite, Nawannaporn Saelim, Monrat Chulanetra, Santi Maneewatch, Wanpen Chaicumpa
A direct acting anti-Ebola agent is needed. VP40, a conserved protein across Ebolavirus (EBOV) species has several pivotal roles in the virus life cycle. Inhibition of VP40 functions would lessen the virion integrity and interfere with the viral assembly, budding, and spread. In this study, cell penetrable human scFvs (HuscFvs) that bound to EBOV VP40 were produced by phage display technology. Gene sequences coding for VP40-bound-HuscFvs were subcloned from phagemids into protein expression plasmids downstream to a gene of cell penetrating peptide, i...
October 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27618260/from-therapeutic-antibodies-to-chimeric-antigen-receptors-cars-making-better-cars-based-on-antigen-binding-domain
#13
Yanling Wu, Shibo Jiang, Tianlei Ying
A variety of approaches are being pursued to improve the safety and antitumor potency of chimeric antigen receptor (CAR) T-cell therapy. However, most engineering efforts have thus far been focused on its intracellular signaling domain, while its extracellular antigen-binding domain has received less attention. Areas covered: Herein, the authors summarize the current knowledge of CAR T-cell therapy. Accordingly, they focus on its antigen-binding domain, discuss key considerations for selecting an optimal single-chain variable fragment (scFv) when designing a CAR, and suggest potential directions aimed at developing the next-generation CARs...
December 2016: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/27617013/human-transbodies-to-hcv-ns3-4a-protease-inhibit-viral-replication-and-restore-host-innate-immunity
#14
Surasak Jittavisutthikul, Watee Seesuay, Jeeraphong Thanongsaksrikul, Kanyarat Thueng-In, Potjanee Srimanote, Rolf G Werner, Wanpen Chaicumpa
A safe and effective direct acting anti-hepatitis C virus (HCV) agent is still needed. In this study, human single chain variable fragments of antibody (scFvs) that bound to HCV NS3/4A protein were produced by phage display technology. The engineered scFvs were linked to nonaarginines (R9) for making them cell penetrable. HCV-RNA-transfected Huh7 cells treated with the transbodies produced from four different transformed E. coli clones had reduced HCV-RNA inside the cells and in the cell spent media, as well as fewer HCV foci in the cell monolayer compared to the transfected cells in culture medium alone...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27614209/retargeting-of-herpes-simplex-virus-hsv-vectors
#15
REVIEW
William F Goins, Bonnie Hall, Justus B Cohen, Joseph C Glorioso
Gene therapy applications depend on vector delivery and gene expression in the appropriate target cell. Vector infection relies on the distribution of natural virus receptors that may either not be present on the desired target cell or distributed in a manner to give off-target gene expression. Some viruses display a very limited host range, while others, including herpes simplex virus (HSV), can infect almost every cell within the human body. It is often an advantage to retarget virus infectivity to achieve selective target cell infection...
December 2016: Current Opinion in Virology
https://www.readbyqxmd.com/read/27606342/%C3%AE-synuclein-conformational-antibodies-fused-to-penetratin-are-effective-in-models-of-lewy-body-disease
#16
Brian Spencer, Stephanie Williams, Edward Rockenstein, Elvira Valera, Wei Xin, Michael Mante, Jazmin Florio, Anthony Adame, Eliezer Masliah, Michael R Sierks
OBJECTIVE: Progressive accumulation of α-synuclein (α-syn) has been associated with Parkinson's disease (PD) and Dementia with Lewy body (DLB). The mechanisms through which α-syn leads to neurodegeneration are not completely clear; however, the formation of various oligomeric species have been proposed to play a role. Antibody therapy has shown effectiveness at reducing α-syn accumulation in the central nervous system (CNS); however, most of these studies have been conducted utilizing antibodies that recognize both monomeric and higher molecular weight α-syn...
August 2016: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/27571429/combining-yeast-display-and-competitive-facs-to-select-rare-hapten-specific-clones-from-recombinant-antibody-libraries
#17
Yue Sun, Bhupal Ban, Andrew Bradbury, G A Shakeel Ansari, Diane A Blake
The development of antibodies to low molecular weight haptens remains challenging due to both the low immunogenicity of many haptens and the cross-reactivity of the protein carriers used to generate the immune response. Recombinant antibodies and novel display technologies have greatly advanced antibody development; however, new techniques are still required to select rare hapten-specific antibodies from large recombinant libraries. In the present study, we used a combination of phage and yeast display to screen an immune antibody library (size, 4...
September 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27558409/novel-nanoscale-bacteriophage-based-single-domain-antibodies-for-the-therapy-of-systemic-infection-caused-by-candida-albicans
#18
Shuai Dong, Hongxi Shi, Donghui Cao, Yicun Wang, Xintong Zhang, Yan Li, Xiang Gao, Li Wang
Candida albicans (C. albicans) is an important human commensal and opportunistic fungal pathogen. Secreted aspartyl proteinases (Saps) are a major virulence trait of C. albicans, and among these proteases Sap2 has the highest expression levels. It is possible that antibodies against Sap2 could provide an antifungal effect. In this study, two phages displaying anti-rSap2 single chain variable fragments (scFvs) were screened from human single fold scFv libraries, and their potential therapeutic roles were evaluated using a murine model infected by C...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27520285/invert-biopanning-a-novel-method-for-efficient-and-rapid-isolation-of-scfvs-by-phage-display-technology
#19
Leila Rahbarnia, Safar Farajnia, Hossein Babaei, Jafar Majidi, Kamal Veisi, Asghar Tanomand, Bahman Akbari
Phage display is a prominent screening technique for development of novel high affinity antibodies against almost any antigen. However, removing false positive clones in screening process remains a challenge. The aim of this study was to develop an efficient and rapid method for isolation of high affinity scFvs by removing NSBs without losing rare specific clones. Therefore, a novel two rounds strategy called invert biopanning was developed for isolating high affinity scFvs against EGFRvIII antigen from human scFv library...
August 9, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27516548/enhancement-of-herg-channel-activity-by-scfv-antibody-fragments-targeted-to-the-pas-domain
#20
Carol A Harley, Greg Starek, David K Jones, Andreia S Fernandes, Gail A Robertson, João H Morais-Cabral
The human human ether-à-go-go-related gene (hERG) potassium channel plays a critical role in the repolarization of the cardiac action potential. Changes in hERG channel function underlie long QT syndrome (LQTS) and are associated with cardiac arrhythmias and sudden death. A striking feature of this channel and KCNH channels in general is the presence of an N-terminal Per-Arnt-Sim (PAS) domain. In other proteins, PAS domains bind ligands and modulate effector domains. However, the PAS domains of KCNH channels are orphan receptors...
August 30, 2016: Proceedings of the National Academy of Sciences of the United States of America
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