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https://www.readbyqxmd.com/read/28165420/behavior-of-gingival-fibroblasts-on-titanium-implant-surfaces-in-combination-with-either-injectable-prf-or-prp
#1
Xuzhu Wang, Yufeng Zhang, Joseph Choukroun, Shahram Ghanaati, Richard J Miron
Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient's own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti-coagulants (injectable-platelet-rich fibrin, i-PRF) was compared to standard platelet-rich plasma (PRP) with gingival fibroblasts cultured on smooth and roughened titanium implant surfaces. Standard PRP and i-PRF (centrifuged at 700 rpm (60× g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), collagen1 (COL1) and fibronectin (FN)...
February 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28154995/injectable-platelet-rich-fibrin-i-prf-opportunities-in-regenerative-dentistry
#2
Richard J Miron, Masako Fujioka-Kobayashi, Maria Hernandez, Umadevi Kandalam, Yufeng Zhang, Shahram Ghanaati, Joseph Choukroun
OBJECTIVES: Platelet rich plasma (PRP) has been utilized in regenerative dentistry as a supra-physiological concentrate of autologous growth factors capable of stimulating tissue regeneration. Despite this, concerns have been expressed regarding the use of anti-coagulants, agents known to inhibit wound healing. In this study, a liquid formulation of platelet rich fibrin (PRF) termed injectable-PRF (i-PRF) without the use of anti-coagulants was investigated. MATERIALS AND METHODS: Standard PRP and i-PRF (centrifuged at 700 rpm (60G) for 3 min) were compared for growth factor release up to 10 days (8 donor samples)...
February 2, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27095909/socket-preservation-by-beta-tri-calcium-phosphate-with-collagen-compared-to-platelet-rich-fibrin-a-clinico-radiographic-study
#3
Swati Das, Rajesh Jhingran, Vivek Kumar Bains, Rohit Madan, Ruchi Srivastava, Iram Rizvi
OBJECTIVES: This study was primarily designed to determine the clinico-radiographic efficacy of platelet-rich fibrin (PRF) and beta-tri-calcium phosphate with collagen (β-TCP-Cl) in preserving extraction sockets. MATERIALS AND METHODS: For Group I (PRF), residual sockets (n = 15) were filled with autologous PRF obtained from patients' blood; and for Group II (β-TCP-Cl), residual sockets (n = 15) were filled with β-TCP-Cl. For the sockets randomly selected for Group II (β-TCP-Cl), the reshaped Resorbable Tissue Replacement cone was inserted into the socket...
April 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/26814997/obtention-of-injectable-platelets-rich-fibrin-i-prf-and-its-polymerization-with-bone-graft-technical-note
#4
Carlos Fernando de Almeida Barros Mourão, Helder Valiense, Elias Rodrigues Melo, Natália Belmock Mascarenhas Freitas Mourão, Mônica Diuana-Calasans Maia
The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP) is the main alternative for use in liquid form (injectable). These injectable form of platelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable) and its use with particulated bone graft materials in the polymerized form...
November 2015: Revista do Colégio Brasileiro de Cirurgiões
https://www.readbyqxmd.com/read/25810590/in-vitro-evaluation-of-mechanical-properties-of-platelet-rich-fibrin-membrane-and-scanning-electron-microscopic-examination-of-its-surface-characteristics
#5
George Sam, Rosamma Joseph Vadakkekuttical, Nagrale Vijay Amol
BACKGROUND: The aim of this study was to evaluate the mechanical properties of the platelet-rich fibrin (PRF) membrane and to compare these properties with that of commercially available collagen membranes used for guided tissue regeneration (GTR) procedures. Scanning electron microscopic (SEM) examination of PRF membrane was also performed to determine the cell distribution pattern within the different regions of the membrane. MATERIALS AND METHODS: Modulus of elasticity and hardness of (i) PRF membrane (ii) bovine collagen membrane and (iii) fish collagen membrane were assessed by performing surface indentation test using T1 950 Triboindenter...
January 2015: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/1333317/tissue-specific-expression-of-the-skeletal-alpha-actin-gene-involves-sequences-that-can-function-independently-of-myod-and-id
#6
COMPARATIVE STUDY
G E Muscat, J Emery, E S Collie
The skeletal alpha-actin gene is a member of the sarcomeric contractile protein gene family and is specifically expressed in differentiated muscle. The skeletal alpha-actin gene is regulated efficiently by enhancer and regulatory sequences between nucleotide positions -1282 and -87. In the present study we have shown that the sequences 3' of nucleotide position -87 can functionally interact with the SV40 enhancer in a tissue-specific manner and can restrict the ubiquitous function of the SV40 enhancer to myogenic cells...
1992: Gene Expression
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