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group b streptococcus screening pregnancy

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https://www.readbyqxmd.com/read/28920845/modified-use-of-real-time-pcr-detection-of-group-b-streptococcus-in-pregnancy
#1
Ali A Rabaan, Justin V Saunar, Ali M Bazzi, Joan L Soriano
The CDC recommends antenatal screening of vaginal/rectal samples for Streptococcus agalactiae at 35-37 weeks' gestation, with intra-partum antibiotic prophylaxis for positive cases. We tested a modified use of the Cepheid Xpert GBS real-time PCR kit on enrichment cultures from 554 vaginal/rectal swabs compared to the current subculturing gold standard method. Swabs were inoculated on polymyxin nalidixic acid agar plates, and Todd-Hewitt enrichment broth cultures were examined daily for growth. Todd-Hewitt broth culture was also used for Xpert GBS...
September 18, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28763075/-group-b-streptococcal-perinatal-infection-a-global-latin-american-and-mexican-overview
#2
Gerardo C Palacios-Saucedo, Talyha Itzel Hernández-Hernández, Lydia Guadalupe Rivera-Morales, Evangelina Briones-Lara, Amílcar Caballero-Trejo, José M Vázquez-Guillén, Gustavo I Amador-Patiño, Ricardo García-Cabello, Fortino Solórzano-Santos, Cristina Rodríguez-Padillacs
Group B streptococci (Streptococcus agalactiae) cause a number of infections in women during pregnancy and postpartum, such as urinary tract infection, chorioamnionitis and endometritis, consequently may affect the newborn. Group B streptococci is the most common cause of severe infections in newborns in developed countries. Studies on the epidemiology of group B streptococci infections in Latin America are still limited. This information is also unknown in Mexico, but studies carried out in the center of the country have found high rates of vaginal colonization in pregnant women and there are case series and case reports of newborns...
May 2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/28762570/streptococcal-toxic-shock-syndrome-occurring-in-the-third-trimester-of-pregnancy-a-case-report
#3
Mohamad Irani, Rodney McLaren, Richard H Savel, Oksana Bogatyryova, Fady Khoury-Collado
Group A streptococcal (GAS) toxic shock syndrome (TSS) is a rare life-threatening illness. Most reported cases have occurred in the post-partum period. Here, we report a rare case of a primigravid who developed GAS TSS in the third trimester. We also review the potential preventive measures and treatment modalities for this syndrome. A 29-year-old primigravid presented at 36 weeks' gestation with diarrhea, abdominal pain, fever, and fetal bradycardia. She underwent an emergency cesarean section and was subsequently diagnosed with GAS TSS...
August 1, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28659318/reliable-detection-of-group-b-streptococcus-in-the-clinical-laboratory
#4
REVIEW
Manuel Rosa-Fraile, Barbara Spellerberg
Group B streptococcus (GBS) is a leading cause of invasive neonatal infections and a significant pathogen in immunocompromised adults. Screening to detect GBS colonization in pregnant women determines the need for antibiotic prophylaxis in that pregnancy. Efficient determination of the GBS colonization status of pregnant women is crucial. Methods that maximize the probability of GBS recovery are needed. The availability of technologies such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular techniques, and chromogenic culture media, including Granada-type media, have changed the scenario for GBS detection and identification...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28633864/perinatal-group-b-streptococcal-infections-virulence-factors-immunity-and-prevention-strategies
#5
REVIEW
Jay Vornhagen, Kristina M Adams Waldorf, Lakshmi Rajagopal
Group B streptococcus (GBS) or Streptococcus agalactiae is a β-hemolytic, Gram-positive bacterium that is a leading cause of neonatal infections. GBS commonly colonizes the lower gastrointestinal and genital tracts and, during pregnancy, neonates are at risk of infection. Although intrapartum antibiotic prophylaxis during labor and delivery has decreased the incidence of early-onset neonatal infection, these measures do not prevent ascending infection that can occur earlier in pregnancy leading to preterm births, stillbirths, or late-onset neonatal infections...
June 17, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28562753/group-b-streptococcus-detection-in-pregnant-women-via-culture-and-pcr-methods
#6
Cláudia Wollheim, Rosa Dea Sperhacke, Sabrina Kahler Ribeiro Fontana, Andréa Cristina Vanni, Sérgio Kakuta Kato, Patricia Regina de Araújo, Afonso Luis Barth, José Mauro Madi
INTRODUCTION: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population...
March 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28395627/advice-against-gbs-screening-approval
#7
(no author information available yet)
The government has been recommended not to approve screening for Group B Streptococcus (GBS) at 35 to 37 weeks of pregnancy so that the bacterium can be treated with antibiotics.
April 11, 2017: Nursing Children and Young People
https://www.readbyqxmd.com/read/28388469/higher-prevalence-of-colonization-with-gardnerella-vaginalis-and-gram-negative-anaerobes-in-patients-with-recurrent-miscarriage-and-elevated-peripheral-natural-killer-cells
#8
R J Kuon, R Togawa, K Vomstein, M Weber, T Goeggl, T Strowitzki, U R Markert, S Zimmermann, V Daniel, A H Dalpke, B Toth
The role of vaginal infections in recurrent miscarriage (RM) is discussed controversially and screening is not recommended in international guidelines. Peripheral and uterine NK cells (pNK, uNK) play an important role in the establishment of a healthy pregnancy and are targets of immune diagnostics in RM patients. The aim of this study was to analyze the composition of the vaginal microbiota in RM patients and to correlate the findings to clinical characteristics as well as NK cell parameters. In total, n=243 RM patients with ≥3 consecutive miscarriages were recruited between 11/2011 and 03/2016...
March 30, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/27911391/a-murine-model-of-group-b-streptococcus-vaginal-colonization
#9
Kathryn A Patras, Kelly S Doran
Streptococcus agalactiae (group B Streptococcus, GBS), is a Gram-positive, asymptomatic colonizer of the human gastrointestinal tract and vaginal tract of 10 - 30% of adults. In immune-compromised individuals, including neonates, pregnant women, and the elderly, GBS may switch to an invasive pathogen causing sepsis, arthritis, pneumonia, and meningitis. Because GBS is a leading bacterial pathogen of neonates, current prophylaxis is comprised of late gestation screening for GBS vaginal colonization and subsequent peripartum antibiotic treatment of GBS-positive mothers...
November 16, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27879969/factors-associated-with-choice-of-approach-for-group-b-streptococcus-screening
#10
H Sefty, A Klivitsky, M Bromberg, R Dichtiar, M Ben Ami, T Shohat, A Glatman-Freedman
BACKGROUND: The crude rate of early-onset Group B streptococcus disease (EOGBS) in Israel has been consistently under 0.5 for 1000 live births for the past 8 years. The Israeli Ministry of Health has adapted the risk factor based approach for preventing EOGBS and universal bacteriological screening for GBS is not recommended. In spite of this policy, there are indications that many pregnant women in Israel undergo bacteriological screening for GBS. The objective of this study is to assess the rate and characteristics of pregnant women who undergo screening for group B streptococcus (GBS) colonization in Israel...
2016: Israel Journal of Health Policy Research
https://www.readbyqxmd.com/read/27558911/group-b-streptococcus-antimicrobial-resistance-in-neonates-born-to-group-b-streptococcus-colonized-mothers-single-center-survey
#11
Yi-Ping Li, Chi-Man Kuok, Shin-Yu Lin, Wu-Shiun Hsieh, Ming-Kwang Shyu
AIM: In this study, we collected group B streptococcus (GBS) screening data and analyzed screening rate, antimicrobial resistance rate, and neonatal observation room (NOR) admission rate due to inadequate chemoprophylaxis. METHODS: The GBS screening data for January 2006-December 2013 were retrospectively collected and analyzed. We also collected data for neonates admitted to NOR due to inadequate chemoprophylaxis during the period 1 April 2010-31 December 2013...
November 2016: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/27451382/-is-rapid-intrapartum-vaginal-screening-test-of-group-b-streptococci-gbs-during-partum-useful-in-identifying-infants-developing-early-onset-gbs-sepsis-in-postpartum-period
#12
J Raignoux, M Benard, S Huo Yung Kai, O Dicky, A Berrebi, L Bibet, A-S Chetouani, N Marty, L Cavalie, C Casper, C Assouline-Azogui
INTRODUCTION: Group B streptococcus (GBS) is the most common infectious agent responsible for early-onset bacterial sepsis (EOS) in term newborns. French prevention of perinatal GBS disease guidelines recommend screening for maternal vaginal GBS colonization at the 9th month of pregnancy, and use of intrapartum antibiotic prophylaxis (IAP) in case of detected GBS vaginal colonization. Peripheral bacterial sampling (gastric aspiration, ear, or meconium) and measurement of C-reactive protein (CRP) are performed in asymptomatic newborns in case of infectious risk factors and/or incomplete IAP...
September 2016: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/27378534/-usefulness-of-a-rapid-intrapartum-real-time-pcr-assay-in-comparison-with-the-group-b-streptococcus-culture-screening-at-the-end-of-pregnancy-in-pregnant-women
#13
M Defez, F Khizar, M Maurin, F Biot, J-C Pons, F Sergent
OBJECTIVES: The objectives were to evaluate and compare the diagnostic accuracy of a rapid real-time PCR assay at the onset of labor with those of the current antenatal culture-based test at 34-38 weeks gestation for group B Streptococcus (GBS) screening. MATERIALS AND METHODS: A prospective study including all pregnant women admitted for delivery after a 34-week gestation period was conducted in October 2012 at the Grenoble University Hospital Centre. A first culture-based GBS screening test was performed between 34 and 38 weeks of gestation followed by a second screening test at the onset of labor, using a real-time PCR Assay and a culture-based method (gold standard) in order to calculate the diagnostic accuracy...
November 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27289215/prediction-of-preterm-delivery-by-second-trimester-inflammatory-biomarkers-in-the-amniotic-fluid
#14
Assaad Kesrouani, Elie Chalhoub, Elie El Rassy, Mirna Germanos, Aline Khazzaka, Jamale Rizkallah, Elie Attieh, Norma Aouad
OBJECTIVE: To search for a correlation between mid-pregnancy altered levels of inflammatory markers and preterm delivery. METHODS: A prospective cohort series included 39 patients undergoing amniocentesis one additional milliliter of amniotic fluid (AF) was stored for later dosage of interleukin-6 (Il-6), matrix metalloproteinase-9 (MMP-9), glucose and C-reactive protein (CRP). Maternal serum CRP and glucose levels were also obtained. Exclusion criteria were multiple pregnancies, chorioamnionitis, group B streptococcus colonization, bacterial vaginosis and cases with proven aneuploidy...
September 2016: Cytokine
https://www.readbyqxmd.com/read/27139805/safety-and-immunogenicity-of-an-investigational-maternal-trivalent-group-b-streptococcus-vaccine-in-healthy-women-and-their-infants-a-randomised-phase-1b-2-trial
#15
RANDOMIZED CONTROLLED TRIAL
Shabir A Madhi, Clare L Cutland, Lisa Jose, Anthonet Koen, Niresha Govender, Frederick Wittke, Morounfolu Olugbosi, Ajoke Sobanjo-Ter Meulen, Sherryl Baker, Peter M Dull, Vas Narasimhan, Karen Slobod
BACKGROUND: Maternal group B streptococcus (GBS) serotype-specific capsular antibody concentrations are correlated with susceptibility to neonatal GBS invasive disease. Maternal immunisation against GBS during pregnancy might protect infants across the period of susceptibility to invasive disease, but no licensed vaccine exists. This study assessed the safety and immunogenicity of a CRM197-conjugated trivalent GBS vaccine in non-pregnant and pregnant women, and antibody transfer to their infants...
August 2016: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/27117365/-rapid-group-b-streptococcus-screening-methods-in-late-pregnancy-and-the-maternal-neonatal-outcomes
#16
X Wang, L K Ma, Y N Song, J T Liu, Y C Xu, J Yi
OBJECTIVE: To study the sensitivity of the quantitative fluorescence polymerase chain reaction (QF-PCR) in detecting group B streptococcus (GBS) in late pregnant women and the influence of vaginal/rectal GBS colonization on the maternal-neonatal outcomes. METHODS: A total of 2020 swabs were taken from 505 women who received routine culture and QF-PCR screening at 35 and 37 weeks of gestation from September, 2013 to April, 2014 at Peking Union Medical College Hospital...
April 19, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/26811897/a-multi-country-cross-sectional-study-of-vaginal-carriage-of-group-b-streptococci-gbs-and-escherichia-coli-in-resource-poor-settings-prevalences-and-risk-factors
#17
Piet Cools, Vicky Jespers, Liselotte Hardy, Tania Crucitti, Sinead Delany-Moretlwe, Mary Mwaura, Gilles F Ndayisaba, Janneke H H M van de Wijgert, Mario Vaneechoutte
BACKGROUND: One million neonates die each year in low- and middle-income countries because of neonatal sepsis; group B Streptococcus (GBS) and Escherichia coli are the leading causes. In sub-Saharan Africa, epidemiological data on vaginal GBS and E. coli carriage, a prerequisite for GBS and E. coli neonatal sepsis, respectively, are scarce but necessary to design and implement prevention strategies. Therefore, we assessed vaginal GBS and E. coli carriage rates and risk factors and the GBS serotype distribution in three sub-Saharan countries...
2016: PloS One
https://www.readbyqxmd.com/read/26683604/efficiency-of-screening-for-the-recurrence-of-antenatal-group-b-streptococcus-colonization-in-a-subsequent-pregnancy-a-systematic-review-and-meta-analysis-with-independent-patient-data
#18
REVIEW
Mark A Turrentine, Laura C Colicchia, Emmet Hirsch, Po-Jen Cheng, Teresa Tam, Patrick S Ramsey, Sarah M Page-Ramsey
OBJECTIVE: The objective of this study was to evaluate the risk of recurrent group B streptococcus (GBS) colonization in a subsequent pregnancy and to assess clinical characteristics that influence this risk. STUDY DESIGN: A systematic review and meta-analysis was performed. Databases were searched from inception through June 2015 using PubMed, Embase, Scopus, Central, and ClinicalTrials.gov. Studies were eligible if they assessed antenatal GBS colonization in two successive pregnancies...
April 2016: American Journal of Perinatology
https://www.readbyqxmd.com/read/26669929/prevalence-of-intervillous-thrombi-is-increased-in-placentas-from-pregnancies-complicated-by-diabetes
#19
Kristen M Basnet, Rhonda Bentley-Lewis, Deborah J Wexler, Fusun Kilic, Drucilla J Roberts
Intervillous thrombus (IVT) is a placental pathology of unclear cause. One possible cause is that IVT protects against fetomaternal transfusion due to trophoblastic disruption. A role for hyperglycemia in trophoblast apoptosis has been suggested. We sought to determine whether placentas from pregnancies complicated by diabetes had an increased incidence of IVT. Medical records of 206 patients with type 1 diabetes (n = 39), type 2 diabetes (n = 37), and gestational diabetes (GDM, n = 130) at the Massachusetts General Hospital were identified...
November 2016: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/26638651/antenatal-gbs-screening-an-australian-perspective
#20
Danielle Clack
Women in Australia are offered a variety of options for screening and treatment of group B streptococcus (GBS), depending on which health service they are engaged with, including a risk-based approach or universal screening. The difficulty for midwives when addressing the evidence supporting these differing policies is that there is no unified national policy on GBS screening in Australia. Furthermore, the Australian population is a discerning one, questioning the implications of procedures and exploring all options...
October 2015: Practising Midwife
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