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fungi bombyx mori bioassay

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https://www.readbyqxmd.com/read/17085713/increased-insect-virulence-in-beauveria-bassiana-strains-overexpressing-an-engineered-chitinase
#1
Yanhua Fan, Weiguo Fang, Shujuan Guo, Xiaoqiong Pei, Yongjun Zhang, Yuehua Xiao, Demou Li, Kai Jin, Michael J Bidochka, Yan Pei
Entomopathogenic fungi are currently being used for the control of several insect pests as alternatives or supplements to chemical insecticides. Improvements in virulence and speed of kill can be achieved by understanding the mechanisms of fungal pathogenesis and genetically modifying targeted genes, thus improving the commercial efficacy of these biocontrol agents. Entomopathogenic fungi, such as Beauveria bassiana, penetrate the insect cuticle utilizing a plethora of hydrolytic enzymes, including chitinases, which are important virulence factors...
January 2007: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/8463709/toxicity-and-pathology-of-crude-broth-filtrate-produced-by-hirsutella-thompsonii-var-thompsonii-in-shake-culture
#2
A Vey, J M Quiot, I Mazet, C W McCoy
Metabolic products secreted by the fungal mycelia of Hirsutella thompsonii var. thompsonii (CBS 556.77D) in a defined culture broth in shake culture were tested for toxicity to Galleria mellonella larvae and Drosophila melanogaster adults via injection and per os application, respectively. In addition, the toxic effect of broth filtrate was observed in vitro in a cell line of Bombyx mori. Czapek-Dox broth fortified with 1% yeast extract stimulated more rapid mycelial growth and correspondingly more toxin production in time...
March 1993: Journal of Invertebrate Pathology
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