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Insect Bioassay

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https://www.readbyqxmd.com/read/28207862/breeding-matters-natal-experience-influences-population-state-dependent-host-acceptance-by-an-eruptive-insect-herbivore
#1
Jordan Lewis Burke, Allan L Carroll
Eruptive forest insects are highly influential agents of change in forest ecosystems, and their effects have increased with recent climate change. State-dependent life histories contribute significantly to the population dynamics of eruptive forest insect herbivores; however, the proximate mechanisms by which these species shift between states is poorly understood. Laboratory bioassays were conducted using the mountain pine beetle (Dendroctonus ponderosae) to determine the effect of maternal host selection on offspring host preferences, as they apply to population state-dependent behaviors...
2017: PloS One
https://www.readbyqxmd.com/read/28206856/effect-of-photoperiod-on-permethrin-resistance-in-aedes-aegypti
#2
O Karina Villanueva, Gustavo Ponce, Beatriz Lopez, Selene M Gutierrez, Iram P Rodriguez, Guadalupe Reyes, Karla J Saavedra, William C Black, Julian Garcia, Barry Beaty, Lars Eisen, Adriana E Flores
Living organisms have been exposed to light-dark cycles that allowed them to adapt to different ecological niches. Circadian cycles affect hormone release, metabolism, and response to xenobiotic compounds. Current studies have shown that insect susceptibility to toxic agents depends on circadian cycles, mainly because the biochemical processes involved in detoxification and responses to oxidative stress are modulated by this process. The goal of this study was to determine the effect of photoperiod on resistance to permethrin in Aedes aegypti ...
December 2016: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28193253/artemisia-spp-essential-oils-against-the-disease-carrying-blowfly-calliphora-vomitoria
#3
Stefano Bedini, Guido Flamini, Francesca Cosci, Roberta Ascrizzi, Maria Cristina Echeverria, Lucia Guidi, Marco Landi, Andrea Lucchi, Barbara Conti
BACKGROUND: Synanthropic flies play a considerable role in the transmission of pathogenic and non-pathogenic microorganisms. In this work, the essential oil (EO) of two aromatic plants, Artemisia annua and Artemisia dracunculus, were evaluated for their abilities to control the blowfly Calliphora vomitoria. Artemisia annua and A. dracunculus EOs were extracted, analysed and tested in laboratory bioassays. Besides, the physiology of EOs toxicity and the EOs antibacterial and antifungal properties were evaluated...
February 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28187835/lethal-and-sublethal-effects-of-the-chitin-synthesis-inhibitor-chlorfluazuron-on-bradysia-odoriphaga-yang-and-zhang-diptera-sciaridae
#4
Peng Zhang, Yun-He Zhao, Qiu-Hong Wang, Wei Mu, Feng Liu
Bradysia odoriphaga Yang and Zhang is the primary insect pest that affects Chinese chive in northern China. Nevertheless, very few studies have been conducted on the use of chitin synthesis inhibitors (CSIs) for the control of B. odoriphaga. Here, lethal and sublethal effects of the CSI chlorfluazuron on B. odoriphaga were studied to explore the use for integrated pest management (IPM) of B. odoriphaga. A contact and ingestion toxicity bioassay showed that chlorfluazuron was more active against B. odoriphaga than three other CSIs, with a 72h LC50 of 0...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28187825/characterization-of-acetylcholinesterase-from-elm-left-beetle-xanthogaleruca-luteola-and-qsar-of-temephos-derivatives-against-its-activity
#5
Mahboobeh Sharifi, Mohammad Ghadamyari, Khodayar Gholivand, Ali Asghar Ebrahimi Valmoozi, Reza H Sajedi
Insect acetylcholinesterase (AChE) is the principal target for organophosphate (OP) and carbamate (CB) insecticides. In this research, an AChE from third instar larvae of elm left beetle, Xanthogaleruca luteola was purified by affinity chromatography. The enzyme was purified 75.29-fold with a total yield of 8.51%. As shown on denaturing SDS-PAGE, the molecular mass of purified AChE was 70kDa. The enzyme demonstrated maximum activity at pH7 and 35°C. Furthermore, a series of temephos (Tem) derivatives with the general structure of P(O)XP(O) (1-44) were prepared, synthesized and characterized by (31)P, (13)C, (1)H NMR and FT-IR spectral techniques...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28187824/expression-of-bacillus-thuringiensis-cytolytic-toxin-cyt2ca1-in-citrus-roots-to-control-diaprepes-abbreviatus-larvae
#6
Sulley Ben Mahmoud, John E Ramos, Robert G Shatters, David G Hall, Stephen L Lapointe, Randall P Niedz, Pierre Rougé, Ronald D Cave, Dov Borovsky
Diaprepes abbreviatus (L.) is an important pest of citrus in the USA. Currently, no effective management strategies of D. abbreviatus exist in citriculture, and new methods of control are desperately sought. To protect citrus against D. abbreviatus a transgenic citrus rootstock expressing Bacillus thuringiensis Cyt2Ca1, an insect toxin protein, was developed using Agrobacterium-mediated transformation of 'Carrizo' citrange [Citrus sinensis (L) Osbeck Poncirus trifoliate (L) Raf]. The transgenic citrus root stock expressed the cytolytic toxin Cyt2Ca1 constitutively under the control of a 35S promoter in the transgenic Carrizo citrange trifoliate hybrid including the roots that are the food source of larval D...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28174591/development-of-a-triple-gene-cry1ac-cry2ab-epsps-construct-and-its-expression-in-nicotiana-benthamiana-for-insect-resistance-and-herbicide-tolerance-in-plants
#7
Rubab Z Naqvi, Muhammad Asif, Muhammad Saeed, Shaheen Asad, Asia Khatoon, Imran Amin, Zahid Mukhtar, Aftab Bashir, Shahid Mansoor
Insect pest complex, cotton leaf curl disease and weeds pose major threat to crop production worldwide, including Pakistan. To address these problems, in the present study a triple gene construct harboring Cry1Ac, Cry2Ab, and EPSPS cassettes has been developed for plant specifically in cotton transformation against lepidopteron insect-pests and weeds. Nicotiana benthamiana (tobacco) was used as a model system for characterization of this triple gene construct. The construct has been assembled in plant expression vector and transformed in N...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28167952/interactions-between-nitrogen-and-silicon-in-rice-and-their-effects-on-resistance-toward-the-brown-planthopper-nilaparvata-lugens
#8
Xiaoying Wu, Yaoguang Yu, Scott R Baerson, Yuanyuan Song, Guohua Liang, Chaohui Ding, Jinbo Niu, Zhiqiang Pan, Rensen Zeng
Nitrogen (N) and silicon (Si) are two important nutritional elements required for plant growth, and both impact host plant resistance toward insect herbivores. The interaction between the two elements may therefore play a significant role in determining host plant resistance. We investigated this interaction in rice (Oryza sativa L.) and its effect on resistance to the herbivore brown planthopper Nilaparvata lugens (BPH). Our results indicate that high-level (5.76 mM) N fertilization reduced Si accumulation in rice leaves, and furthermore, this decrease was likely due to decreased expression of Si transporters OsLsi1 and OsLsi2...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28132659/long-chain-alkanes-and-fatty-acids-from-ludwigia-octovalvis-weed-leaf-surface-waxes-as-short-range-attractant-and-ovipositional-stimulant-to-altica-cyanea-weber-coleoptera-chrysomelidae
#9
S Mitra, N Sarkar, A Barik
The importance of leaf surface wax compounds from the rice-field weed Ludwigia octovalvis (Jacq.) Raven (Onagraceae) was determined in the flea beetle Altica cyanea (Weber) (Coleoptera: Chrysomelidae). Extraction, thin layer chromatography and GC-MS and GC-FID analyses of surface waxes of young, mature and senescent leaves revealed 20, 19 and 19 n-alkanes between n-C15 and n-C35, respectively; whereas 14, 14 and 12 free fatty acids between C12:0 and C22:0 fatty acids were identified in young, mature and senescent leaves, respectively...
January 30, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28081289/transgenic-cotton-expressing-cry10aa-toxin-confers-high-resistance-to-the-cotton-boll-weevil
#10
Thuanne Pires Ribeiro, Fabricio Barbosa Monteiro Arraes, Isabela Tristan Lourenço-Tessutti, Marilia Santos Silva, Maria Eugênia Lisei-de-Sá, Wagner Alexandre Lucena, Leonardo Lima Pepino Macedo, Janaina Nascimento Lima, Regina Maria Santos Amorim, Sinara Artico, Márcio Alves-Ferreira, Maria Cristina Mattar Silva, Maria Fatima Grossi-de-Sa
Genetically modified (GM) cotton events that effectively control cotton boll weevil (CBW), which is the most destructive cotton insect pest in South America, are reported here for the first time. This work presents the successful development of new GM cotton with high resistance to CBW conferred by Cry10Aa toxin, a protein encoded by entomopathogenic Bacillus thuringiensis (Bt) gene. The plant transformation vector harbouring cry10Aa gene driven by the cotton ubiquitination-related promoter uceA1.7 was introduced into a Brazilian cotton cultivar by biolistic transformation...
January 12, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28062794/larvicidal-and-growth-inhibitory-activity-of-entomopathogenic-bacteria-culture-fluids-against-aedes-aegypti-diptera-culicidae
#11
João Luiz Rosa da Silva, Fernanda Undurraga Schwalm, Carlos Eugênio Silva, Marisa da Costa, Ralf Heermann, Onilda Santos da Silva
Dengue, Chikungunya, and Zika are important vector-borne diseases, and Aedes aegypti L. is their main transmitter. As the disease management is mainly based on mosquito control strategies, the search for alternative and cost-effective approaches is ongoing. The Gram-negative bacteria Xenorhabdus nematophila and Photorhabdus luminescens are symbiotically associated with entomopathogenic nematodes and are highly pathogenic for insect larvae. After we have recently confirmed the toxicity of these bacteria in Ae...
January 6, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28060922/cry3bb1-resistant-western-corn-rootworm-diabrotica-virgifera-virgifera-leconte-does-not-exhibit-cross-resistance-to-dvsnf7-dsrna
#12
William Moar, Chitvan Khajuria, Michael Pleau, Oliver Ilagan, Mao Chen, Changjian Jiang, Paula Price, Brian McNulty, Thomas Clark, Graham Head
BACKGROUND AND METHODOLOGY: There is a continuing need to express new insect control compounds in transgenic maize against western corn rootworm, Diabrotica virgifera virgifera (LeConte) (WCR). In this study three experiments were conducted to determine cross-resistance between the new insecticidal DvSnf7 dsRNA, and Bacillus thuringiensis (Bt) Cry3Bb1; used to control WCR since 2003, with field-evolved resistance being reported. Laboratory susceptible and Cry3Bb1-resistant WCR were evaluated against DvSnf7 dsRNA in larval diet-incorporation bioassays...
2017: PloS One
https://www.readbyqxmd.com/read/28050633/biopolymer-microencapsulations-of-bacillus-thuringiensis-crystal-preparations-for-increased-stability-and-resistance-to-environmental-stress
#13
Xiaolin He, Zhongqin Sun, Kanglai He, Shuyuan Guo
Parasporal crystals synthesized by Bacillus thuringiensis (Bt) have been widely used as microbial pesticides because of their toxicity to the larval stages of specific insects. However, parasporal crystals can be damaged by environmental stresses, such as high temperature, ultraviolet radiation, and desiccation. To reduce environmental susceptibility of parasporal crystals and extend the duration of their activity, we developed a new type of protection by making microcapsules of crystals (MCs). The microcapsules were self-assembled by alternate deposition (layer by layer) of low-cost chitosan and sodium alginate (or sodium carboxymethyl cellulose) on the crystal surface...
January 3, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28045942/knock-down-of-whitefly-gut-gene-expression-and-mortality-by-orally-delivered-gut-gene-specific-dsrnas
#14
Meenal Vyas, Amir Raza, Muhammad Yousaf Ali, Muhammad Aleem Ashraf, Shahid Mansoor, Ahmad Ali Shahid, Judith K Brown
Control of the whitefly Bemisia tabaci (Genn.) agricultural pest and plant virus vector relies on the use of chemical insecticides. RNA-interference (RNAi) is a homology-dependent innate immune response in eukaryotes, including insects, which results in degradation of the corresponding transcript following its recognition by a double-stranded RNA (dsRNA) that shares 100% sequence homology. In this study, six whitefly 'gut' genes were selected from an in silico-annotated transcriptome library constructed from the whitefly alimentary canal or 'gut' of the B biotype of B...
2017: PloS One
https://www.readbyqxmd.com/read/28045087/nutrition-affects-insect-susceptibility-to-bt-toxins
#15
Carrie A Deans, Spencer T Behmer, Ashley E Tessnow, Patricia Tamez-Guerra, Marianne Pusztai-Carey, Gregory A Sword
Pesticide resistance represents a major challenge to global food production. The spread of resistance alleles is the primary explanation for observations of reduced pesticide efficacy over time, but the potential for gene-by-environment interactions (plasticity) to mediate susceptibility has largely been overlooked. Here we show that nutrition is an environmental factor that affects susceptibility to Bt toxins. Protein and carbohydrates are two key macronutrients for insect herbivores, and the polyphagous pest Helicoverpa zea self-selects and performs best on diets that are protein-biased relative to carbohydrates...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28042104/laboratory-bioassays-with-three-different-substrates-to-test-the-efficacy-of-insecticides-against-various-stages-of-drosophila-suzukii-diptera-drosophilidae
#16
Aneliya Koleva Pavlova, Melanie Dahlmann, Mirjam Hauck, Annette Reineke
Rapid worldwide spread and polyphagous nature of the spotted wing Drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae) calls for efficient and selective control strategies to prevent severe economic losses in various fruit crops. The use of insecticides is one option for management of this invasive pest insect. Efficacy of insecticides is usually assessed first in laboratory bioassays, which are compounded by the cryptic nature of D. suzukii larvae and the fact that fruits used in bioassays often start to rot and dissolve before larvae have reached the adult stage...
January 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28032105/evaluation-of-some-plant-essential-oils-against-the-brown-banded-cockroach-supella-longipalpa-blattaria-ectobiidae-a-mechanical-vector-of-human-pathogens
#17
Mona Sharififard, Farhad Safdari, Amir Siahpoush, Hamid Kassiri
BACKGROUND: Essential oils, as secondary plant compounds, present a safer alternative to conventional insecticides in insect control programs. So five essential oils including eucalyptus, mint, yarrow, oregano and rosemary oils were evaluated against the brown-banded cockroach Supella longipalpa. METHODS: Evaluation was done against the 3(rd) and 4(th) instar nymphs using three bioassay methods; continuous contact toxicity, fumigant toxicity and repellent activity...
December 2016: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/28019074/identification-and-detection-of-indoxacarb-resistance-mutations-in-the-para-sodium-channel-of-the-tomato-leafminer-tuta-absoluta
#18
Emmanouil Roditakis, Konstantinos Mavridis, Maria Riga, Emmanouil Vasakis, Evangelia Morou, Jean Luc Rison, John Vontas
BACKGROUND: Indoxacarb is an important active ingredient extensively used for the control of Tuta absoluta, a major tomato pest, holding a particular role in insecticide resistance management schemes. RESULTS: Reduced susceptibility to indoxacarb was identified (1794-fold resistance), through toxicological bioassays in a field population from Greece and evolved rapidly to resistance after short laboratory selection. Combined bioassays with synergists and biochemical analysis suggested only a partial involvement of detoxification enzymes in the resistant phenotype...
December 26, 2016: Pest Management Science
https://www.readbyqxmd.com/read/28011736/toxicological-enzymatic-and-molecular-assessment-of-the-insecticide-susceptibility-profile-of-triatoma-infestans-hemiptera-reduviidae-triatominae-populations-from-rural-communities-of-santa-cruz-bolivia
#19
Pablo L Santo-Orihuela, Claudia V Vassena, Guillermo Carvajal, Eva Clark, Silvio Menacho, Ricardo Bozo, Robert H Gilman, Caryn Bern, Paula L Marcet
A wide range of insecticide resistance profiles has been reported across Bolivian domestic and sylvatic populations of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae), including some with levels proven to be a threat for vector control. In this work, the insecticide profile of domestic T. infestans was studied with standardized toxicological bioassays, in an area that has not undergone consistent vector control. F1 first-instar nymphs hatched in laboratory from bugs captured in three communities from the Santa Cruz Department were evaluated with different insecticides...
December 22, 2016: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28011730/arrestant-effect-of-human-scalp-components-on-head-louse-phthiraptera-pediculidae-behavior
#20
Isabel Ortega-Insaurralde, Ariel Ceferino Toloza, Paola Gonzalez-Audino, María Inés Picollo
Relevant evidence has shown that parasites process host-related information using chemical, visual, tactile, or auditory cues. However, the cues that are involved in the host-parasite interaction between Pediculus humanus capitis (De Geer 1767) and humans have not been identified yet. In this work, we studied the effect of human scalp components on the behavior of adult head lice. Filter paper segments were rubbed on volunteers' scalps and then placed in the experimental arena, where adult head lice were individually tested...
December 23, 2016: Journal of Medical Entomology
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